SLF1 cDNA ORF clone, Calidris pugnax(ruff)

The following SLF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa61262 XM_014936862.1
Latest version!
Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa61263 XM_014936863.1
Latest version!
Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa61264 XM_014936864.1
Latest version!
Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa61264 XM_014936865.1
Latest version!
Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa61262
    Clone ID Related Accession (Same CDS sequence) XM_014936862.1
    Accession Version XM_014936862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product SMC5-SMC6 complex localization factor protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTTGTC AGATGGCAAG TGACGGCCAG AAGCGGGTAG TCCAATTTAC CGGATTTAAA 
    AAGCGAGAGA AAGCGGCGCT GCTGAAATGG CTCTTCAGAC TGGACTGTGT TTTTTTACCT
    GATAAGAAAT ACAGGAACTG CACACATCTT ATAGCAAAGA AGCCGTGCAA GAGTGAAAAG
    TTCTTAGCTG CCTGTGCTGC TGGAAAGTGG ATACTAACTA AGGAGTACAT AATTAATAGT
    GCCGAAAGTG GCAGATGGCT TGATGAAACA ACGTACGAAT GGGGATATGA AATTGAAAAA
    GACACCCATT ATTCACCTCA AAGGCAATCT GCACCTAAAA GATGGCGTGA AGAACTGACA
    CGCTGGAGTG CTCCAGGGGC CTTCCACAGA TGGAAAGTTG TCCTCCCTGC TAAGGAAGGT
    GATAAACGAA TGGCGTCTAT TAGAAGAGTT CTTCAAGCTG GAAAGGCAAC CATATGTTCA
    TCTCAAAAGG CAGAGTGCGG TATAACTCAT GTATTCATCC TTAATAAAAT TTGTCCTGTG
    CAAAACAAAA AAGGTCTCTC GGAAGCTCGA TACTACCCTG TGCAATATCT GGGAGATTAC
    CTTTTTGAGA ATGAGATACA AAATACTGAA GATATCCTGG CTGCACAAGA AACCAACCAA
    ATCATGTCTA ATACACAGCT CTCAGCAATG AGAAGTGCTG TTATTAAACA TATGTATTTT
    GCTGTGACTG TTAAGCACAG GATTGTTCAA AAAGACCAGC TGAATAAATG CAGTCCAGAG
    GTTGAAAATA CTACCCTGTC CAGAGGCACA TGGTATATGC TTGAAGGATT AATGGAAGAG
    TATTTATTTC CCGATGTAAT CACAGAACTT GCTGCTGGTC AAAAGTACTC TACACCTCCT
    GTGCAACTTC TTCATTTTCT TCTAGAATAC GCCCTGTTGG GAAGTACTGA TGCAATATTT
    TATGCTAAAC TTCATCATGC TTTGTACCTT GTTCTCCATT GTGATCCTCC TTGGAAGTCC
    TCATCTATGT TAAAGTATTA TCTGGATCTT CTCCAGTGTC CTATCTGTAA GAAAGGTACA
    TGGTCCTTCA TAGAGATGCT GGTAAGGTCT TGCCTTTATT GCAAAACCAT TTGTCATGGA
    GTCTCGGTTT CAGGGAGAGG AGATGAACAG AGGAGAGTTC ACAAAACATT ATTGAAGTTT
    TTCTTTGATG TAGTTAAAGC TGAAGTAGAG TTTCTGACGA AAAGTTTGGT TGAAGGGACC
    GATTCGCAGC ATCAGCAAGT CATGCCACAG ACAGTCCTCC TGAAGACCTT TTGGCTGGGA
    GGCGAAACCT CGGTGCTTTT TACCAAACAC ATAAATATTC TAACAGACTG GGTCATACTT
    TCCCATCGAG AATTGAACAG AAGGAACGAT GCTTTCACAT GTGAAATAGC TGGTCTATTA
    AATGCAATTC TTGGAACTGT AGTGGAGTAT TGGATCATCT CAAGTTTGCT TATGAACAGA
    AATATGTTTC ATCAAATAGC TGATGATTTG GCTCACTACA TTGCCATTTC CTGTGATGAT
    TTTTCAATAA GTGAATTGAA AATGTTCATT TCTTCCATAC CATCCCGTTG GCTTCAAATG
    TTTGTTGCAG AAGCAGTTTT CAAAAAAATG TGTTTACAGA GGAATATAGC TATTTCTAAA
    GAACCTCTTT CTCTTCAGAA AATAGTCTGT TGCTATTTGC CTGCTTTGCG AGAGGTGGGG
    ATGCAGGAGA CCAGGAGGAT GCAGAAAGCA AAGAAAACGA AAATAGGGCA ACGGCCGTGC
    CCTGAGTCTC AGAGGGCATT ACAGATGCTA TATGGTGATA AGCAGAACCA AGTCAAAGTG
    CTGCCTGATC TACCTGACTT AATCTTTAAT AAGTGCCCTA CTTTGACAAA GAAGCTTAGA
    ACACAACCAG AAGTTGAGGC TTCATACACC AAAGAGAACT GCTACGTTTT GGCCGAAGAG
    GTACATTTTT ACAAGAGAAA TCTTAAAGGA GAGACAGCCC TGCATAGAGC TTGCATAAGA
    AACAAAGTGG AGAGATTGAT TCAGCTTCTC TCTTCCCCTG GAATAGACAT TAATGTTAAA
    GACTATGCTG GCTGGACGCC TTTGCATGAA GCCTGTAACC ATGGCAGCAC AGTGTGTGTC
    CGTGAAATTT TGCAGCGTTG TCCAGAGGTA GACCTGCTCA GTCAAGTGGA CGGGGTGACT
    CCTTTGCATG ATGCACTGTC AAACGGACAC GTAGAAATTG GCAAGCTGCT CCTTCAGCAC
    GGGGGACCAG TCCTTCTACA GCAGAGGGAC TCCAATGGAG AACTGCCACT GGATTATGTT
    GAGTCCGAAG CTGTAAAGCA AGAACTCCTG AATGTTGTTC ATCCAGAAGA GAACATTGAC
    AGCTTCTGTG AACGCACAGA ACAAGATTTT TGCAGTCAGC AGGGAGAACT GTGGTTGATT
    TTATTTAATA AGATGCTGCT GAATTTCTGT TCGGTTTATA ATCTGTCTTC ACCCTTTACT
    TTAACTCTCA GAGAGGTAGC TTGTTCAAAT GCACTTCCAG TAATGGCTTG TGATAACTGT
    AGGATGAAAA ATAGATTTTC TGCAGATTGG TTAGTAGATA CATATTTTAA AGAGCTAGAA
    ACTTTTCAAA AGCTACCAGA GTTTCTTGAA GAAATATCTG CAAACATGTT GTTTTTCCCC
    GGAGAACAGA TGAATGCTTT ATTAGCAACT CTGGAAACTA TGGTCGAGGC ATATCGGCTC
    TTTACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792348.1
    CDS56..2824
    Translation

    Target ORF information:

    RefSeq Version XM_014936862.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTTGTC AGATGGCAAG TGACGGCCAG AAGCGGGTAG TCCAATTTAC CGGATTTAAA 
    AAGCGAGAGA AAGCGGCGCT GCTGAAATGG CTCTTCAGAC TGGACTGTGT TTTTTTACCT
    GATAAGAAAT ACAGGAACTG CACACATCTT ATAGCAAAGA AGCCGTGCAA GAGTGAAAAG
    TTCTTAGCTG CCTGTGCTGC TGGAAAGTGG ATACTAACTA AGGAGTACAT AATTAATAGT
    GCCGAAAGTG GCAGATGGCT TGATGAAACA ACGTACGAAT GGGGATATGA AATTGAAAAA
    GACACCCATT ATTCACCTCA AAGGCAATCT GCACCTAAAA GATGGCGTGA AGAACTGACA
    CGCTGGAGTG CTCCAGGGGC CTTCCACAGA TGGAAAGTTG TCCTCCCTGC TAAGGAAGGT
    GATAAACGAA TGGCGTCTAT TAGAAGAGTT CTTCAAGCTG GAAAGGCAAC CATATGTTCA
    TCTCAAAAGG CAGAGTGCGG TATAACTCAT GTATTCATCC TTAATAAAAT TTGTCCTGTG
    CAAAACAAAA AAGGTCTCTC GGAAGCTCGA TACTACCCTG TGCAATATCT GGGAGATTAC
    CTTTTTGAGA ATGAGATACA AAATACTGAA GATATCCTGG CTGCACAAGA AACCAACCAA
    ATCATGTCTA ATACACAGCT CTCAGCAATG AGAAGTGCTG TTATTAAACA TATGTATTTT
    GCTGTGACTG TTAAGCACAG GATTGTTCAA AAAGACCAGC TGAATAAATG CAGTCCAGAG
    GTTGAAAATA CTACCCTGTC CAGAGGCACA TGGTATATGC TTGAAGGATT AATGGAAGAG
    TATTTATTTC CCGATGTAAT CACAGAACTT GCTGCTGGTC AAAAGTACTC TACACCTCCT
    GTGCAACTTC TTCATTTTCT TCTAGAATAC GCCCTGTTGG GAAGTACTGA TGCAATATTT
    TATGCTAAAC TTCATCATGC TTTGTACCTT GTTCTCCATT GTGATCCTCC TTGGAAGTCC
    TCATCTATGT TAAAGTATTA TCTGGATCTT CTCCAGTGTC CTATCTGTAA GAAAGGTACA
    TGGTCCTTCA TAGAGATGCT GGTAAGGTCT TGCCTTTATT GCAAAACCAT TTGTCATGGA
    GTCTCGGTTT CAGGGAGAGG AGATGAACAG AGGAGAGTTC ACAAAACATT ATTGAAGTTT
    TTCTTTGATG TAGTTAAAGC TGAAGTAGAG TTTCTGACGA AAAGTTTGGT TGAAGGGACC
    GATTCGCAGC ATCAGCAAGT CATGCCACAG ACAGTCCTCC TGAAGACCTT TTGGCTGGGA
    GGCGAAACCT CGGTGCTTTT TACCAAACAC ATAAATATTC TAACAGACTG GGTCATACTT
    TCCCATCGAG AATTGAACAG AAGGAACGAT GCTTTCACAT GTGAAATAGC TGGTCTATTA
    AATGCAATTC TTGGAACTGT AGTGGAGTAT TGGATCATCT CAAGTTTGCT TATGAACAGA
    AATATGTTTC ATCAAATAGC TGATGATTTG GCTCACTACA TTGCCATTTC CTGTGATGAT
    TTTTCAATAA GTGAATTGAA AATGTTCATT TCTTCCATAC CATCCCGTTG GCTTCAAATG
    TTTGTTGCAG AAGCAGTTTT CAAAAAAATG TGTTTACAGA GGAATATAGC TATTTCTAAA
    GAACCTCTTT CTCTTCAGAA AATAGTCTGT TGCTATTTGC CTGCTTTGCG AGAGGTGGGG
    ATGCAGGAGA CCAGGAGGAT GCAGAAAGCA AAGAAAACGA AAATAGGGCA ACGGCCGTGC
    CCTGAGTCTC AGAGGGCATT ACAGATGCTA TATGGTGATA AGCAGAACCA AGTCAAAGTG
    CTGCCTGATC TACCTGACTT AATCTTTAAT AAGTGCCCTA CTTTGACAAA GAAGCTTAGA
    ACACAACCAG AAGTTGAGGC TTCATACACC AAAGAGAACT GCTACGTTTT GGCCGAAGAG
    GTACATTTTT ACAAGAGAAA TCTTAAAGGA GAGACAGCCC TGCATAGAGC TTGCATAAGA
    AACAAAGTGG AGAGATTGAT TCAGCTTCTC TCTTCCCCTG GAATAGACAT TAATGTTAAA
    GACTATGCTG GCTGGACGCC TTTGCATGAA GCCTGTAACC ATGGCAGCAC AGTGTGTGTC
    CGTGAAATTT TGCAGCGTTG TCCAGAGGTA GACCTGCTCA GTCAAGTGGA CGGGGTGACT
    CCTTTGCATG ATGCACTGTC AAACGGACAC GTAGAAATTG GCAAGCTGCT CCTTCAGCAC
    GGGGGACCAG TCCTTCTACA GCAGAGGGAC TCCAATGGAG AACTGCCACT GGATTATGTT
    GAGTCCGAAG CTGTAAAGCA AGAACTCCTG AATGTTGTTC ATCCAGAAGA GAACATTGAC
    AGCTTCTGTG AACGCACAGA ACAAGATTTT TGCAGTCAGC AGGGAGAACT GTGGTTGATT
    TTATTTAATA AGATGCTGCT GAATTTCTGT TCGGTTTATA ATCTGTCTTC ACCCTTTACT
    TTAACTCTCA GAGAGGTAGC TTGTTCAAAT GCACTTCCAG TAATGGCTTG TGATAACTGT
    AGGATGAAAA ATAGATTTTC TGCAGATTGG TTAGTAGATA CATATTTTAA AGAGCTAGAA
    ACTTTTCAAA AGCTACCAGA GTTTCTTGAA GAAATATCTG CAAACATGTT GTTTTTCCCC
    GGAGAACAGA TGAATGCTTT ATTAGCAACT CTGGAAACTA TGGTCGAGGC ATATCGGCTC
    TTTACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa61263
    Clone ID Related Accession (Same CDS sequence) XM_014936863.1
    Accession Version XM_014936863.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product SMC5-SMC6 complex localization factor protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTTGTA TGGCAAGTGA CGGCCAGAAG CGGGTAGTCC AATTTACCGG ATTTAAAAAG 
    CGAGAGAAAG CGGCGCTGCT GAAATGGCTC TTCAGACTGG ACTGTGTTTT TTTACCTGAT
    AAGAAATACA GGAACTGCAC ACATCTTATA GCAAAGAAGC CGTGCAAGAG TGAAAAGTTC
    TTAGCTGCCT GTGCTGCTGG AAAGTGGATA CTAACTAAGG AGTACATAAT TAATAGTGCC
    GAAAGTGGCA GATGGCTTGA TGAAACAACG TACGAATGGG GATATGAAAT TGAAAAAGAC
    ACCCATTATT CACCTCAAAG GCAATCTGCA CCTAAAAGAT GGCGTGAAGA ACTGACACGC
    TGGAGTGCTC CAGGGGCCTT CCACAGATGG AAAGTTGTCC TCCCTGCTAA GGAAGGTGAT
    AAACGAATGG CGTCTATTAG AAGAGTTCTT CAAGCTGGAA AGGCAACCAT ATGTTCATCT
    CAAAAGGCAG AGTGCGGTAT AACTCATGTA TTCATCCTTA ATAAAATTTG TCCTGTGCAA
    AACAAAAAAG GTCTCTCGGA AGCTCGATAC TACCCTGTGC AATATCTGGG AGATTACCTT
    TTTGAGAATG AGATACAAAA TACTGAAGAT ATCCTGGCTG CACAAGAAAC CAACCAAATC
    ATGTCTAATA CACAGCTCTC AGCAATGAGA AGTGCTGTTA TTAAACATAT GTATTTTGCT
    GTGACTGTTA AGCACAGGAT TGTTCAAAAA GACCAGCTGA ATAAATGCAG TCCAGAGGTT
    GAAAATACTA CCCTGTCCAG AGGCACATGG TATATGCTTG AAGGATTAAT GGAAGAGTAT
    TTATTTCCCG ATGTAATCAC AGAACTTGCT GCTGGTCAAA AGTACTCTAC ACCTCCTGTG
    CAACTTCTTC ATTTTCTTCT AGAATACGCC CTGTTGGGAA GTACTGATGC AATATTTTAT
    GCTAAACTTC ATCATGCTTT GTACCTTGTT CTCCATTGTG ATCCTCCTTG GAAGTCCTCA
    TCTATGTTAA AGTATTATCT GGATCTTCTC CAGTGTCCTA TCTGTAAGAA AGGTACATGG
    TCCTTCATAG AGATGCTGGT AAGGTCTTGC CTTTATTGCA AAACCATTTG TCATGGAGTC
    TCGGTTTCAG GGAGAGGAGA TGAACAGAGG AGAGTTCACA AAACATTATT GAAGTTTTTC
    TTTGATGTAG TTAAAGCTGA AGTAGAGTTT CTGACGAAAA GTTTGGTTGA AGGGACCGAT
    TCGCAGCATC AGCAAGTCAT GCCACAGACA GTCCTCCTGA AGACCTTTTG GCTGGGAGGC
    GAAACCTCGG TGCTTTTTAC CAAACACATA AATATTCTAA CAGACTGGGT CATACTTTCC
    CATCGAGAAT TGAACAGAAG GAACGATGCT TTCACATGTG AAATAGCTGG TCTATTAAAT
    GCAATTCTTG GAACTGTAGT GGAGTATTGG ATCATCTCAA GTTTGCTTAT GAACAGAAAT
    ATGTTTCATC AAATAGCTGA TGATTTGGCT CACTACATTG CCATTTCCTG TGATGATTTT
    TCAATAAGTG AATTGAAAAT GTTCATTTCT TCCATACCAT CCCGTTGGCT TCAAATGTTT
    GTTGCAGAAG CAGTTTTCAA AAAAATGTGT TTACAGAGGA ATATAGCTAT TTCTAAAGAA
    CCTCTTTCTC TTCAGAAAAT AGTCTGTTGC TATTTGCCTG CTTTGCGAGA GGTGGGGATG
    CAGGAGACCA GGAGGATGCA GAAAGCAAAG AAAACGAAAA TAGGGCAACG GCCGTGCCCT
    GAGTCTCAGA GGGCATTACA GATGCTATAT GGTGATAAGC AGAACCAAGT CAAAGTGCTG
    CCTGATCTAC CTGACTTAAT CTTTAATAAG TGCCCTACTT TGACAAAGAA GCTTAGAACA
    CAACCAGAAG TTGAGGCTTC ATACACCAAA GAGAACTGCT ACGTTTTGGC CGAAGAGGTA
    CATTTTTACA AGAGAAATCT TAAAGGAGAG ACAGCCCTGC ATAGAGCTTG CATAAGAAAC
    AAAGTGGAGA GATTGATTCA GCTTCTCTCT TCCCCTGGAA TAGACATTAA TGTTAAAGAC
    TATGCTGGCT GGACGCCTTT GCATGAAGCC TGTAACCATG GCAGCACAGT GTGTGTCCGT
    GAAATTTTGC AGCGTTGTCC AGAGGTAGAC CTGCTCAGTC AAGTGGACGG GGTGACTCCT
    TTGCATGATG CACTGTCAAA CGGACACGTA GAAATTGGCA AGCTGCTCCT TCAGCACGGG
    GGACCAGTCC TTCTACAGCA GAGGGACTCC AATGGAGAAC TGCCACTGGA TTATGTTGAG
    TCCGAAGCTG TAAAGCAAGA ACTCCTGAAT GTTGTTCATC CAGAAGAGAA CATTGACAGC
    TTCTGTGAAC GCACAGAACA AGATTTTTGC AGTCAGCAGG GAGAACTGTG GTTGATTTTA
    TTTAATAAGA TGCTGCTGAA TTTCTGTTCG GTTTATAATC TGTCTTCACC CTTTACTTTA
    ACTCTCAGAG AGGTAGCTTG TTCAAATGCA CTTCCAGTAA TGGCTTGTGA TAACTGTAGG
    ATGAAAAATA GATTTTCTGC AGATTGGTTA GTAGATACAT ATTTTAAAGA GCTAGAAACT
    TTTCAAAAGC TACCAGAGTT TCTTGAAGAA ATATCTGCAA ACATGTTGTT TTTCCCCGGA
    GAACAGATGA ATGCTTTATT AGCAACTCTG GAAACTATGG TCGAGGCATA TCGGCTCTTT
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792349.1
    CDS63..2828
    Translation

    Target ORF information:

    RefSeq Version XM_014936863.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTTGTA TGGCAAGTGA CGGCCAGAAG CGGGTAGTCC AATTTACCGG ATTTAAAAAG 
    CGAGAGAAAG CGGCGCTGCT GAAATGGCTC TTCAGACTGG ACTGTGTTTT TTTACCTGAT
    AAGAAATACA GGAACTGCAC ACATCTTATA GCAAAGAAGC CGTGCAAGAG TGAAAAGTTC
    TTAGCTGCCT GTGCTGCTGG AAAGTGGATA CTAACTAAGG AGTACATAAT TAATAGTGCC
    GAAAGTGGCA GATGGCTTGA TGAAACAACG TACGAATGGG GATATGAAAT TGAAAAAGAC
    ACCCATTATT CACCTCAAAG GCAATCTGCA CCTAAAAGAT GGCGTGAAGA ACTGACACGC
    TGGAGTGCTC CAGGGGCCTT CCACAGATGG AAAGTTGTCC TCCCTGCTAA GGAAGGTGAT
    AAACGAATGG CGTCTATTAG AAGAGTTCTT CAAGCTGGAA AGGCAACCAT ATGTTCATCT
    CAAAAGGCAG AGTGCGGTAT AACTCATGTA TTCATCCTTA ATAAAATTTG TCCTGTGCAA
    AACAAAAAAG GTCTCTCGGA AGCTCGATAC TACCCTGTGC AATATCTGGG AGATTACCTT
    TTTGAGAATG AGATACAAAA TACTGAAGAT ATCCTGGCTG CACAAGAAAC CAACCAAATC
    ATGTCTAATA CACAGCTCTC AGCAATGAGA AGTGCTGTTA TTAAACATAT GTATTTTGCT
    GTGACTGTTA AGCACAGGAT TGTTCAAAAA GACCAGCTGA ATAAATGCAG TCCAGAGGTT
    GAAAATACTA CCCTGTCCAG AGGCACATGG TATATGCTTG AAGGATTAAT GGAAGAGTAT
    TTATTTCCCG ATGTAATCAC AGAACTTGCT GCTGGTCAAA AGTACTCTAC ACCTCCTGTG
    CAACTTCTTC ATTTTCTTCT AGAATACGCC CTGTTGGGAA GTACTGATGC AATATTTTAT
    GCTAAACTTC ATCATGCTTT GTACCTTGTT CTCCATTGTG ATCCTCCTTG GAAGTCCTCA
    TCTATGTTAA AGTATTATCT GGATCTTCTC CAGTGTCCTA TCTGTAAGAA AGGTACATGG
    TCCTTCATAG AGATGCTGGT AAGGTCTTGC CTTTATTGCA AAACCATTTG TCATGGAGTC
    TCGGTTTCAG GGAGAGGAGA TGAACAGAGG AGAGTTCACA AAACATTATT GAAGTTTTTC
    TTTGATGTAG TTAAAGCTGA AGTAGAGTTT CTGACGAAAA GTTTGGTTGA AGGGACCGAT
    TCGCAGCATC AGCAAGTCAT GCCACAGACA GTCCTCCTGA AGACCTTTTG GCTGGGAGGC
    GAAACCTCGG TGCTTTTTAC CAAACACATA AATATTCTAA CAGACTGGGT CATACTTTCC
    CATCGAGAAT TGAACAGAAG GAACGATGCT TTCACATGTG AAATAGCTGG TCTATTAAAT
    GCAATTCTTG GAACTGTAGT GGAGTATTGG ATCATCTCAA GTTTGCTTAT GAACAGAAAT
    ATGTTTCATC AAATAGCTGA TGATTTGGCT CACTACATTG CCATTTCCTG TGATGATTTT
    TCAATAAGTG AATTGAAAAT GTTCATTTCT TCCATACCAT CCCGTTGGCT TCAAATGTTT
    GTTGCAGAAG CAGTTTTCAA AAAAATGTGT TTACAGAGGA ATATAGCTAT TTCTAAAGAA
    CCTCTTTCTC TTCAGAAAAT AGTCTGTTGC TATTTGCCTG CTTTGCGAGA GGTGGGGATG
    CAGGAGACCA GGAGGATGCA GAAAGCAAAG AAAACGAAAA TAGGGCAACG GCCGTGCCCT
    GAGTCTCAGA GGGCATTACA GATGCTATAT GGTGATAAGC AGAACCAAGT CAAAGTGCTG
    CCTGATCTAC CTGACTTAAT CTTTAATAAG TGCCCTACTT TGACAAAGAA GCTTAGAACA
    CAACCAGAAG TTGAGGCTTC ATACACCAAA GAGAACTGCT ACGTTTTGGC CGAAGAGGTA
    CATTTTTACA AGAGAAATCT TAAAGGAGAG ACAGCCCTGC ATAGAGCTTG CATAAGAAAC
    AAAGTGGAGA GATTGATTCA GCTTCTCTCT TCCCCTGGAA TAGACATTAA TGTTAAAGAC
    TATGCTGGCT GGACGCCTTT GCATGAAGCC TGTAACCATG GCAGCACAGT GTGTGTCCGT
    GAAATTTTGC AGCGTTGTCC AGAGGTAGAC CTGCTCAGTC AAGTGGACGG GGTGACTCCT
    TTGCATGATG CACTGTCAAA CGGACACGTA GAAATTGGCA AGCTGCTCCT TCAGCACGGG
    GGACCAGTCC TTCTACAGCA GAGGGACTCC AATGGAGAAC TGCCACTGGA TTATGTTGAG
    TCCGAAGCTG TAAAGCAAGA ACTCCTGAAT GTTGTTCATC CAGAAGAGAA CATTGACAGC
    TTCTGTGAAC GCACAGAACA AGATTTTTGC AGTCAGCAGG GAGAACTGTG GTTGATTTTA
    TTTAATAAGA TGCTGCTGAA TTTCTGTTCG GTTTATAATC TGTCTTCACC CTTTACTTTA
    ACTCTCAGAG AGGTAGCTTG TTCAAATGCA CTTCCAGTAA TGGCTTGTGA TAACTGTAGG
    ATGAAAAATA GATTTTCTGC AGATTGGTTA GTAGATACAT ATTTTAAAGA GCTAGAAACT
    TTTCAAAAGC TACCAGAGTT TCTTGAAGAA ATATCTGCAA ACATGTTGTT TTTCCCCGGA
    GAACAGATGA ATGCTTTATT AGCAACTCTG GAAACTATGG TCGAGGCATA TCGGCTCTTT
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa61264
    Clone ID Related Accession (Same CDS sequence) XM_014936864.1 , XM_014936865.1
    Accession Version XM_014936864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product SMC5-SMC6 complex localization factor protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAGTG ACGGCCAGAA GCGGGTAGTC CAATTTACCG GATTTAAAAA GCGAGAGAAA 
    GCGGCGCTGC TGAAATGGCT CTTCAGACTG GACTGTGTTT TTTTACCTGA TAAGAAATAC
    AGGAACTGCA CACATCTTAT AGCAAAGAAG CCGTGCAAGA GTGAAAAGTT CTTAGCTGCC
    TGTGCTGCTG GAAAGTGGAT ACTAACTAAG GAGTACATAA TTAATAGTGC CGAAAGTGGC
    AGATGGCTTG ATGAAACAAC GTACGAATGG GGATATGAAA TTGAAAAAGA CACCCATTAT
    TCACCTCAAA GGCAATCTGC ACCTAAAAGA TGGCGTGAAG AACTGACACG CTGGAGTGCT
    CCAGGGGCCT TCCACAGATG GAAAGTTGTC CTCCCTGCTA AGGAAGGTGA TAAACGAATG
    GCGTCTATTA GAAGAGTTCT TCAAGCTGGA AAGGCAACCA TATGTTCATC TCAAAAGGCA
    GAGTGCGGTA TAACTCATGT ATTCATCCTT AATAAAATTT GTCCTGTGCA AAACAAAAAA
    GGTCTCTCGG AAGCTCGATA CTACCCTGTG CAATATCTGG GAGATTACCT TTTTGAGAAT
    GAGATACAAA ATACTGAAGA TATCCTGGCT GCACAAGAAA CCAACCAAAT CATGTCTAAT
    ACACAGCTCT CAGCAATGAG AAGTGCTGTT ATTAAACATA TGTATTTTGC TGTGACTGTT
    AAGCACAGGA TTGTTCAAAA AGACCAGCTG AATAAATGCA GTCCAGAGGT TGAAAATACT
    ACCCTGTCCA GAGGCACATG GTATATGCTT GAAGGATTAA TGGAAGAGTA TTTATTTCCC
    GATGTAATCA CAGAACTTGC TGCTGGTCAA AAGTACTCTA CACCTCCTGT GCAACTTCTT
    CATTTTCTTC TAGAATACGC CCTGTTGGGA AGTACTGATG CAATATTTTA TGCTAAACTT
    CATCATGCTT TGTACCTTGT TCTCCATTGT GATCCTCCTT GGAAGTCCTC ATCTATGTTA
    AAGTATTATC TGGATCTTCT CCAGTGTCCT ATCTGTAAGA AAGGTACATG GTCCTTCATA
    GAGATGCTGG TAAGGTCTTG CCTTTATTGC AAAACCATTT GTCATGGAGT CTCGGTTTCA
    GGGAGAGGAG ATGAACAGAG GAGAGTTCAC AAAACATTAT TGAAGTTTTT CTTTGATGTA
    GTTAAAGCTG AAGTAGAGTT TCTGACGAAA AGTTTGGTTG AAGGGACCGA TTCGCAGCAT
    CAGCAAGTCA TGCCACAGAC AGTCCTCCTG AAGACCTTTT GGCTGGGAGG CGAAACCTCG
    GTGCTTTTTA CCAAACACAT AAATATTCTA ACAGACTGGG TCATACTTTC CCATCGAGAA
    TTGAACAGAA GGAACGATGC TTTCACATGT GAAATAGCTG GTCTATTAAA TGCAATTCTT
    GGAACTGTAG TGGAGTATTG GATCATCTCA AGTTTGCTTA TGAACAGAAA TATGTTTCAT
    CAAATAGCTG ATGATTTGGC TCACTACATT GCCATTTCCT GTGATGATTT TTCAATAAGT
    GAATTGAAAA TGTTCATTTC TTCCATACCA TCCCGTTGGC TTCAAATGTT TGTTGCAGAA
    GCAGTTTTCA AAAAAATGTG TTTACAGAGG AATATAGCTA TTTCTAAAGA ACCTCTTTCT
    CTTCAGAAAA TAGTCTGTTG CTATTTGCCT GCTTTGCGAG AGGTGGGGAT GCAGGAGACC
    AGGAGGATGC AGAAAGCAAA GAAAACGAAA ATAGGGCAAC GGCCGTGCCC TGAGTCTCAG
    AGGGCATTAC AGATGCTATA TGGTGATAAG CAGAACCAAG TCAAAGTGCT GCCTGATCTA
    CCTGACTTAA TCTTTAATAA GTGCCCTACT TTGACAAAGA AGCTTAGAAC ACAACCAGAA
    GTTGAGGCTT CATACACCAA AGAGAACTGC TACGTTTTGG CCGAAGAGGT ACATTTTTAC
    AAGAGAAATC TTAAAGGAGA GACAGCCCTG CATAGAGCTT GCATAAGAAA CAAAGTGGAG
    AGATTGATTC AGCTTCTCTC TTCCCCTGGA ATAGACATTA ATGTTAAAGA CTATGCTGGC
    TGGACGCCTT TGCATGAAGC CTGTAACCAT GGCAGCACAG TGTGTGTCCG TGAAATTTTG
    CAGCGTTGTC CAGAGGTAGA CCTGCTCAGT CAAGTGGACG GGGTGACTCC TTTGCATGAT
    GCACTGTCAA ACGGACACGT AGAAATTGGC AAGCTGCTCC TTCAGCACGG GGGACCAGTC
    CTTCTACAGC AGAGGGACTC CAATGGAGAA CTGCCACTGG ATTATGTTGA GTCCGAAGCT
    GTAAAGCAAG AACTCCTGAA TGTTGTTCAT CCAGAAGAGA ACATTGACAG CTTCTGTGAA
    CGCACAGAAC AAGATTTTTG CAGTCAGCAG GGAGAACTGT GGTTGATTTT ATTTAATAAG
    ATGCTGCTGA ATTTCTGTTC GGTTTATAAT CTGTCTTCAC CCTTTACTTT AACTCTCAGA
    GAGGTAGCTT GTTCAAATGC ACTTCCAGTA ATGGCTTGTG ATAACTGTAG GATGAAAAAT
    AGATTTTCTG CAGATTGGTT AGTAGATACA TATTTTAAAG AGCTAGAAAC TTTTCAAAAG
    CTACCAGAGT TTCTTGAAGA AATATCTGCA AACATGTTGT TTTTCCCCGG AGAACAGATG
    AATGCTTTAT TAGCAACTCT GGAAACTATG GTCGAGGCAT ATCGGCTCTT TACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792350.1
    CDS19..2775
    Translation

    Target ORF information:

    RefSeq Version XM_014936864.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAGTG ACGGCCAGAA GCGGGTAGTC CAATTTACCG GATTTAAAAA GCGAGAGAAA 
    GCGGCGCTGC TGAAATGGCT CTTCAGACTG GACTGTGTTT TTTTACCTGA TAAGAAATAC
    AGGAACTGCA CACATCTTAT AGCAAAGAAG CCGTGCAAGA GTGAAAAGTT CTTAGCTGCC
    TGTGCTGCTG GAAAGTGGAT ACTAACTAAG GAGTACATAA TTAATAGTGC CGAAAGTGGC
    AGATGGCTTG ATGAAACAAC GTACGAATGG GGATATGAAA TTGAAAAAGA CACCCATTAT
    TCACCTCAAA GGCAATCTGC ACCTAAAAGA TGGCGTGAAG AACTGACACG CTGGAGTGCT
    CCAGGGGCCT TCCACAGATG GAAAGTTGTC CTCCCTGCTA AGGAAGGTGA TAAACGAATG
    GCGTCTATTA GAAGAGTTCT TCAAGCTGGA AAGGCAACCA TATGTTCATC TCAAAAGGCA
    GAGTGCGGTA TAACTCATGT ATTCATCCTT AATAAAATTT GTCCTGTGCA AAACAAAAAA
    GGTCTCTCGG AAGCTCGATA CTACCCTGTG CAATATCTGG GAGATTACCT TTTTGAGAAT
    GAGATACAAA ATACTGAAGA TATCCTGGCT GCACAAGAAA CCAACCAAAT CATGTCTAAT
    ACACAGCTCT CAGCAATGAG AAGTGCTGTT ATTAAACATA TGTATTTTGC TGTGACTGTT
    AAGCACAGGA TTGTTCAAAA AGACCAGCTG AATAAATGCA GTCCAGAGGT TGAAAATACT
    ACCCTGTCCA GAGGCACATG GTATATGCTT GAAGGATTAA TGGAAGAGTA TTTATTTCCC
    GATGTAATCA CAGAACTTGC TGCTGGTCAA AAGTACTCTA CACCTCCTGT GCAACTTCTT
    CATTTTCTTC TAGAATACGC CCTGTTGGGA AGTACTGATG CAATATTTTA TGCTAAACTT
    CATCATGCTT TGTACCTTGT TCTCCATTGT GATCCTCCTT GGAAGTCCTC ATCTATGTTA
    AAGTATTATC TGGATCTTCT CCAGTGTCCT ATCTGTAAGA AAGGTACATG GTCCTTCATA
    GAGATGCTGG TAAGGTCTTG CCTTTATTGC AAAACCATTT GTCATGGAGT CTCGGTTTCA
    GGGAGAGGAG ATGAACAGAG GAGAGTTCAC AAAACATTAT TGAAGTTTTT CTTTGATGTA
    GTTAAAGCTG AAGTAGAGTT TCTGACGAAA AGTTTGGTTG AAGGGACCGA TTCGCAGCAT
    CAGCAAGTCA TGCCACAGAC AGTCCTCCTG AAGACCTTTT GGCTGGGAGG CGAAACCTCG
    GTGCTTTTTA CCAAACACAT AAATATTCTA ACAGACTGGG TCATACTTTC CCATCGAGAA
    TTGAACAGAA GGAACGATGC TTTCACATGT GAAATAGCTG GTCTATTAAA TGCAATTCTT
    GGAACTGTAG TGGAGTATTG GATCATCTCA AGTTTGCTTA TGAACAGAAA TATGTTTCAT
    CAAATAGCTG ATGATTTGGC TCACTACATT GCCATTTCCT GTGATGATTT TTCAATAAGT
    GAATTGAAAA TGTTCATTTC TTCCATACCA TCCCGTTGGC TTCAAATGTT TGTTGCAGAA
    GCAGTTTTCA AAAAAATGTG TTTACAGAGG AATATAGCTA TTTCTAAAGA ACCTCTTTCT
    CTTCAGAAAA TAGTCTGTTG CTATTTGCCT GCTTTGCGAG AGGTGGGGAT GCAGGAGACC
    AGGAGGATGC AGAAAGCAAA GAAAACGAAA ATAGGGCAAC GGCCGTGCCC TGAGTCTCAG
    AGGGCATTAC AGATGCTATA TGGTGATAAG CAGAACCAAG TCAAAGTGCT GCCTGATCTA
    CCTGACTTAA TCTTTAATAA GTGCCCTACT TTGACAAAGA AGCTTAGAAC ACAACCAGAA
    GTTGAGGCTT CATACACCAA AGAGAACTGC TACGTTTTGG CCGAAGAGGT ACATTTTTAC
    AAGAGAAATC TTAAAGGAGA GACAGCCCTG CATAGAGCTT GCATAAGAAA CAAAGTGGAG
    AGATTGATTC AGCTTCTCTC TTCCCCTGGA ATAGACATTA ATGTTAAAGA CTATGCTGGC
    TGGACGCCTT TGCATGAAGC CTGTAACCAT GGCAGCACAG TGTGTGTCCG TGAAATTTTG
    CAGCGTTGTC CAGAGGTAGA CCTGCTCAGT CAAGTGGACG GGGTGACTCC TTTGCATGAT
    GCACTGTCAA ACGGACACGT AGAAATTGGC AAGCTGCTCC TTCAGCACGG GGGACCAGTC
    CTTCTACAGC AGAGGGACTC CAATGGAGAA CTGCCACTGG ATTATGTTGA GTCCGAAGCT
    GTAAAGCAAG AACTCCTGAA TGTTGTTCAT CCAGAAGAGA ACATTGACAG CTTCTGTGAA
    CGCACAGAAC AAGATTTTTG CAGTCAGCAG GGAGAACTGT GGTTGATTTT ATTTAATAAG
    ATGCTGCTGA ATTTCTGTTC GGTTTATAAT CTGTCTTCAC CCTTTACTTT AACTCTCAGA
    GAGGTAGCTT GTTCAAATGC ACTTCCAGTA ATGGCTTGTG ATAACTGTAG GATGAAAAAT
    AGATTTTCTG CAGATTGGTT AGTAGATACA TATTTTAAAG AGCTAGAAAC TTTTCAAAAG
    CTACCAGAGT TTCTTGAAGA AATATCTGCA AACATGTTGT TTTTCCCCGG AGAACAGATG
    AATGCTTTAT TAGCAACTCT GGAAACTATG GTCGAGGCAT ATCGGCTCTT TACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa61264
    Clone ID Related Accession (Same CDS sequence) XM_014936864.1 , XM_014936865.1
    Accession Version XM_014936865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product SMC5-SMC6 complex localization factor protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAGTG ACGGCCAGAA GCGGGTAGTC CAATTTACCG GATTTAAAAA GCGAGAGAAA 
    GCGGCGCTGC TGAAATGGCT CTTCAGACTG GACTGTGTTT TTTTACCTGA TAAGAAATAC
    AGGAACTGCA CACATCTTAT AGCAAAGAAG CCGTGCAAGA GTGAAAAGTT CTTAGCTGCC
    TGTGCTGCTG GAAAGTGGAT ACTAACTAAG GAGTACATAA TTAATAGTGC CGAAAGTGGC
    AGATGGCTTG ATGAAACAAC GTACGAATGG GGATATGAAA TTGAAAAAGA CACCCATTAT
    TCACCTCAAA GGCAATCTGC ACCTAAAAGA TGGCGTGAAG AACTGACACG CTGGAGTGCT
    CCAGGGGCCT TCCACAGATG GAAAGTTGTC CTCCCTGCTA AGGAAGGTGA TAAACGAATG
    GCGTCTATTA GAAGAGTTCT TCAAGCTGGA AAGGCAACCA TATGTTCATC TCAAAAGGCA
    GAGTGCGGTA TAACTCATGT ATTCATCCTT AATAAAATTT GTCCTGTGCA AAACAAAAAA
    GGTCTCTCGG AAGCTCGATA CTACCCTGTG CAATATCTGG GAGATTACCT TTTTGAGAAT
    GAGATACAAA ATACTGAAGA TATCCTGGCT GCACAAGAAA CCAACCAAAT CATGTCTAAT
    ACACAGCTCT CAGCAATGAG AAGTGCTGTT ATTAAACATA TGTATTTTGC TGTGACTGTT
    AAGCACAGGA TTGTTCAAAA AGACCAGCTG AATAAATGCA GTCCAGAGGT TGAAAATACT
    ACCCTGTCCA GAGGCACATG GTATATGCTT GAAGGATTAA TGGAAGAGTA TTTATTTCCC
    GATGTAATCA CAGAACTTGC TGCTGGTCAA AAGTACTCTA CACCTCCTGT GCAACTTCTT
    CATTTTCTTC TAGAATACGC CCTGTTGGGA AGTACTGATG CAATATTTTA TGCTAAACTT
    CATCATGCTT TGTACCTTGT TCTCCATTGT GATCCTCCTT GGAAGTCCTC ATCTATGTTA
    AAGTATTATC TGGATCTTCT CCAGTGTCCT ATCTGTAAGA AAGGTACATG GTCCTTCATA
    GAGATGCTGG TAAGGTCTTG CCTTTATTGC AAAACCATTT GTCATGGAGT CTCGGTTTCA
    GGGAGAGGAG ATGAACAGAG GAGAGTTCAC AAAACATTAT TGAAGTTTTT CTTTGATGTA
    GTTAAAGCTG AAGTAGAGTT TCTGACGAAA AGTTTGGTTG AAGGGACCGA TTCGCAGCAT
    CAGCAAGTCA TGCCACAGAC AGTCCTCCTG AAGACCTTTT GGCTGGGAGG CGAAACCTCG
    GTGCTTTTTA CCAAACACAT AAATATTCTA ACAGACTGGG TCATACTTTC CCATCGAGAA
    TTGAACAGAA GGAACGATGC TTTCACATGT GAAATAGCTG GTCTATTAAA TGCAATTCTT
    GGAACTGTAG TGGAGTATTG GATCATCTCA AGTTTGCTTA TGAACAGAAA TATGTTTCAT
    CAAATAGCTG ATGATTTGGC TCACTACATT GCCATTTCCT GTGATGATTT TTCAATAAGT
    GAATTGAAAA TGTTCATTTC TTCCATACCA TCCCGTTGGC TTCAAATGTT TGTTGCAGAA
    GCAGTTTTCA AAAAAATGTG TTTACAGAGG AATATAGCTA TTTCTAAAGA ACCTCTTTCT
    CTTCAGAAAA TAGTCTGTTG CTATTTGCCT GCTTTGCGAG AGGTGGGGAT GCAGGAGACC
    AGGAGGATGC AGAAAGCAAA GAAAACGAAA ATAGGGCAAC GGCCGTGCCC TGAGTCTCAG
    AGGGCATTAC AGATGCTATA TGGTGATAAG CAGAACCAAG TCAAAGTGCT GCCTGATCTA
    CCTGACTTAA TCTTTAATAA GTGCCCTACT TTGACAAAGA AGCTTAGAAC ACAACCAGAA
    GTTGAGGCTT CATACACCAA AGAGAACTGC TACGTTTTGG CCGAAGAGGT ACATTTTTAC
    AAGAGAAATC TTAAAGGAGA GACAGCCCTG CATAGAGCTT GCATAAGAAA CAAAGTGGAG
    AGATTGATTC AGCTTCTCTC TTCCCCTGGA ATAGACATTA ATGTTAAAGA CTATGCTGGC
    TGGACGCCTT TGCATGAAGC CTGTAACCAT GGCAGCACAG TGTGTGTCCG TGAAATTTTG
    CAGCGTTGTC CAGAGGTAGA CCTGCTCAGT CAAGTGGACG GGGTGACTCC TTTGCATGAT
    GCACTGTCAA ACGGACACGT AGAAATTGGC AAGCTGCTCC TTCAGCACGG GGGACCAGTC
    CTTCTACAGC AGAGGGACTC CAATGGAGAA CTGCCACTGG ATTATGTTGA GTCCGAAGCT
    GTAAAGCAAG AACTCCTGAA TGTTGTTCAT CCAGAAGAGA ACATTGACAG CTTCTGTGAA
    CGCACAGAAC AAGATTTTTG CAGTCAGCAG GGAGAACTGT GGTTGATTTT ATTTAATAAG
    ATGCTGCTGA ATTTCTGTTC GGTTTATAAT CTGTCTTCAC CCTTTACTTT AACTCTCAGA
    GAGGTAGCTT GTTCAAATGC ACTTCCAGTA ATGGCTTGTG ATAACTGTAG GATGAAAAAT
    AGATTTTCTG CAGATTGGTT AGTAGATACA TATTTTAAAG AGCTAGAAAC TTTTCAAAAG
    CTACCAGAGT TTCTTGAAGA AATATCTGCA AACATGTTGT TTTTCCCCGG AGAACAGATG
    AATGCTTTAT TAGCAACTCT GGAAACTATG GTCGAGGCAT ATCGGCTCTT TACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792351.1
    CDS20..2776
    Translation

    Target ORF information:

    RefSeq Version XM_014936865.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SMC5-SMC6 complex localization factor 1 (SLF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAGTG ACGGCCAGAA GCGGGTAGTC CAATTTACCG GATTTAAAAA GCGAGAGAAA 
    GCGGCGCTGC TGAAATGGCT CTTCAGACTG GACTGTGTTT TTTTACCTGA TAAGAAATAC
    AGGAACTGCA CACATCTTAT AGCAAAGAAG CCGTGCAAGA GTGAAAAGTT CTTAGCTGCC
    TGTGCTGCTG GAAAGTGGAT ACTAACTAAG GAGTACATAA TTAATAGTGC CGAAAGTGGC
    AGATGGCTTG ATGAAACAAC GTACGAATGG GGATATGAAA TTGAAAAAGA CACCCATTAT
    TCACCTCAAA GGCAATCTGC ACCTAAAAGA TGGCGTGAAG AACTGACACG CTGGAGTGCT
    CCAGGGGCCT TCCACAGATG GAAAGTTGTC CTCCCTGCTA AGGAAGGTGA TAAACGAATG
    GCGTCTATTA GAAGAGTTCT TCAAGCTGGA AAGGCAACCA TATGTTCATC TCAAAAGGCA
    GAGTGCGGTA TAACTCATGT ATTCATCCTT AATAAAATTT GTCCTGTGCA AAACAAAAAA
    GGTCTCTCGG AAGCTCGATA CTACCCTGTG CAATATCTGG GAGATTACCT TTTTGAGAAT
    GAGATACAAA ATACTGAAGA TATCCTGGCT GCACAAGAAA CCAACCAAAT CATGTCTAAT
    ACACAGCTCT CAGCAATGAG AAGTGCTGTT ATTAAACATA TGTATTTTGC TGTGACTGTT
    AAGCACAGGA TTGTTCAAAA AGACCAGCTG AATAAATGCA GTCCAGAGGT TGAAAATACT
    ACCCTGTCCA GAGGCACATG GTATATGCTT GAAGGATTAA TGGAAGAGTA TTTATTTCCC
    GATGTAATCA CAGAACTTGC TGCTGGTCAA AAGTACTCTA CACCTCCTGT GCAACTTCTT
    CATTTTCTTC TAGAATACGC CCTGTTGGGA AGTACTGATG CAATATTTTA TGCTAAACTT
    CATCATGCTT TGTACCTTGT TCTCCATTGT GATCCTCCTT GGAAGTCCTC ATCTATGTTA
    AAGTATTATC TGGATCTTCT CCAGTGTCCT ATCTGTAAGA AAGGTACATG GTCCTTCATA
    GAGATGCTGG TAAGGTCTTG CCTTTATTGC AAAACCATTT GTCATGGAGT CTCGGTTTCA
    GGGAGAGGAG ATGAACAGAG GAGAGTTCAC AAAACATTAT TGAAGTTTTT CTTTGATGTA
    GTTAAAGCTG AAGTAGAGTT TCTGACGAAA AGTTTGGTTG AAGGGACCGA TTCGCAGCAT
    CAGCAAGTCA TGCCACAGAC AGTCCTCCTG AAGACCTTTT GGCTGGGAGG CGAAACCTCG
    GTGCTTTTTA CCAAACACAT AAATATTCTA ACAGACTGGG TCATACTTTC CCATCGAGAA
    TTGAACAGAA GGAACGATGC TTTCACATGT GAAATAGCTG GTCTATTAAA TGCAATTCTT
    GGAACTGTAG TGGAGTATTG GATCATCTCA AGTTTGCTTA TGAACAGAAA TATGTTTCAT
    CAAATAGCTG ATGATTTGGC TCACTACATT GCCATTTCCT GTGATGATTT TTCAATAAGT
    GAATTGAAAA TGTTCATTTC TTCCATACCA TCCCGTTGGC TTCAAATGTT TGTTGCAGAA
    GCAGTTTTCA AAAAAATGTG TTTACAGAGG AATATAGCTA TTTCTAAAGA ACCTCTTTCT
    CTTCAGAAAA TAGTCTGTTG CTATTTGCCT GCTTTGCGAG AGGTGGGGAT GCAGGAGACC
    AGGAGGATGC AGAAAGCAAA GAAAACGAAA ATAGGGCAAC GGCCGTGCCC TGAGTCTCAG
    AGGGCATTAC AGATGCTATA TGGTGATAAG CAGAACCAAG TCAAAGTGCT GCCTGATCTA
    CCTGACTTAA TCTTTAATAA GTGCCCTACT TTGACAAAGA AGCTTAGAAC ACAACCAGAA
    GTTGAGGCTT CATACACCAA AGAGAACTGC TACGTTTTGG CCGAAGAGGT ACATTTTTAC
    AAGAGAAATC TTAAAGGAGA GACAGCCCTG CATAGAGCTT GCATAAGAAA CAAAGTGGAG
    AGATTGATTC AGCTTCTCTC TTCCCCTGGA ATAGACATTA ATGTTAAAGA CTATGCTGGC
    TGGACGCCTT TGCATGAAGC CTGTAACCAT GGCAGCACAG TGTGTGTCCG TGAAATTTTG
    CAGCGTTGTC CAGAGGTAGA CCTGCTCAGT CAAGTGGACG GGGTGACTCC TTTGCATGAT
    GCACTGTCAA ACGGACACGT AGAAATTGGC AAGCTGCTCC TTCAGCACGG GGGACCAGTC
    CTTCTACAGC AGAGGGACTC CAATGGAGAA CTGCCACTGG ATTATGTTGA GTCCGAAGCT
    GTAAAGCAAG AACTCCTGAA TGTTGTTCAT CCAGAAGAGA ACATTGACAG CTTCTGTGAA
    CGCACAGAAC AAGATTTTTG CAGTCAGCAG GGAGAACTGT GGTTGATTTT ATTTAATAAG
    ATGCTGCTGA ATTTCTGTTC GGTTTATAAT CTGTCTTCAC CCTTTACTTT AACTCTCAGA
    GAGGTAGCTT GTTCAAATGC ACTTCCAGTA ATGGCTTGTG ATAACTGTAG GATGAAAAAT
    AGATTTTCTG CAGATTGGTT AGTAGATACA TATTTTAAAG AGCTAGAAAC TTTTCAAAAG
    CTACCAGAGT TTCTTGAAGA AATATCTGCA AACATGTTGT TTTTCCCCGG AGAACAGATG
    AATGCTTTAT TAGCAACTCT GGAAACTATG GTCGAGGCAT ATCGGCTCTT TACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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