ORF » Species Summary » Calidris pugnax » MYLK cDNA ORF clone

MYLK cDNA ORF clone, Calidris pugnax(ruff)

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa58139 XM_014939845.1
Latest version!		 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OCa58140 XM_014939852.1
Latest version!		 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OCa58137 XM_014939830.1
Latest version!		 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCa58138 XM_014939837.1
Latest version!		 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MYLK ( XM_014939845.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795331.1 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCa58139
    Clone ID Related Accession (Same CDS sequence) XM_014939845.1
    Accession Version XM_014939845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090770.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAAGCCA AAAAACTTTC CAAAGATAGG ATGAAGAAAT ATATGGCCAG AAGAAAATGG 
    CAGAAAACAG GCCATGCTGT CCGAGCAATA GGAAGGCTGT CGTCCATGGC AATGATTTCT 
    GGTATGAGTG GGAGGAAGGC CTCTGGGAGC TCGCCTACCA GCCCTATAAA TGCAGACAAA 
    GTAGAGAATG AAGATGCTTT CCTTGAAGAA GTGGCTGAAG AAAAGCCCCA TGTAAAGCCA 
    TATTTTACTA AAAAAATCCT TGACATAGAG GTTGTGGAAG GAAGTGCTGC TAGATTTGAC 
    TGCAAAATTG AAGGCTATCC TGACCCTGAG GTGATGTGGT ACAAAGATGA TCAGCCTGTC 
    AAGGAGTCCC GTCACTTCCA GATAGATTAC GATGAGGAGG GGAACTGTTC TTTGACTATT 
    GCTGAAGTTT GTGGGGACGA TGATGCCAAA TACACCTGCA AAGCTGTTAA CAGCCTCGGG 
    GAAGCCACGT GCACCGCAGA ACTCCTTGTG GAAACGATGG AAGGAAAAGA AGAAGAAGAA 
    GATGAGGAGG AGGAAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014795331.1
    CDS116..676
    Translation

    Target ORF information:

    RefSeq Version XM_014939845.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014939845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAAGCCA AAAAACTTTC CAAAGATAGG ATGAAGAAAT ATATGGCCAG AAGAAAATGG 
    CAGAAAACAG GCCATGCTGT CCGAGCAATA GGAAGGCTGT CGTCCATGGC AATGATTTCT 
    GGTATGAGTG GGAGGAAGGC CTCTGGGAGC TCGCCTACCA GCCCTATAAA TGCAGACAAA 
    GTAGAGAATG AAGATGCTTT CCTTGAAGAA GTGGCTGAAG AAAAGCCCCA TGTAAAGCCA 
    TATTTTACTA AAAAAATCCT TGACATAGAG GTTGTGGAAG GAAGTGCTGC TAGATTTGAC 
    TGCAAAATTG AAGGCTATCC TGACCCTGAG GTGATGTGGT ACAAAGATGA TCAGCCTGTC 
    AAGGAGTCCC GTCACTTCCA GATAGATTAC GATGAGGAGG GGAACTGTTC TTTGACTATT 
    GCTGAAGTTT GTGGGGACGA TGATGCCAAA TACACCTGCA AAGCTGTTAA CAGCCTCGGG 
    GAAGCCACGT GCACCGCAGA ACTCCTTGTG GAAACGATGG AAGGAAAAGA AGAAGAAGAA 
    GATGAGGAGG AGGAAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MYLK ( XM_014939852.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795338.1 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OCa58140
    Clone ID Related Accession (Same CDS sequence) XM_014939852.1
    Accession Version XM_014939852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090770.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCT 
    ATAAATGCAG ACAAAGTAGA GAATGAAGAT GCTTTCCTTG AAGAAGTGGC TGAAGAAAAG 
    CCCCATGTAA AGCCATATTT TACTAAAAAA ATCCTTGACA TAGAGGTTGT GGAAGGAAGT 
    GCTGCTAGAT TTGACTGCAA AATTGAAGGC TATCCTGACC CTGAGGTGAT GTGGTACAAA 
    GATGATCAGC CTGTCAAGGA GTCCCGTCAC TTCCAGATAG ATTACGATGA GGAGGGGAAC 
    TGTTCTTTGA CTATTGCTGA AGTTTGTGGG GACGATGATG CCAAATACAC CTGCAAAGCT 
    GTTAACAGCC TCGGGGAAGC CACGTGCACC GCAGAACTCC TTGTGGAAAC GATGGAAGGA 
    AAAGAAGAAG AAGAAGATGA GGAGGAGGAA GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014795338.1
    CDS211..666
    Translation

    Target ORF information:

    RefSeq Version XM_014939852.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014939852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCT 
    ATAAATGCAG ACAAAGTAGA GAATGAAGAT GCTTTCCTTG AAGAAGTGGC TGAAGAAAAG 
    CCCCATGTAA AGCCATATTT TACTAAAAAA ATCCTTGACA TAGAGGTTGT GGAAGGAAGT 
    GCTGCTAGAT TTGACTGCAA AATTGAAGGC TATCCTGACC CTGAGGTGAT GTGGTACAAA 
    GATGATCAGC CTGTCAAGGA GTCCCGTCAC TTCCAGATAG ATTACGATGA GGAGGGGAAC 
    TGTTCTTTGA CTATTGCTGA AGTTTGTGGG GACGATGATG CCAAATACAC CTGCAAAGCT 
    GTTAACAGCC TCGGGGAAGC CACGTGCACC GCAGAACTCC TTGTGGAAAC GATGGAAGGA 
    AAAGAAGAAG AAGAAGATGA GGAGGAGGAA GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MYLK ( XM_014939830.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795316.1 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa58137
    Clone ID Related Accession (Same CDS sequence) XM_014939830.1
    Accession Version XM_014939830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090770.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGGATG TGAAACTGGT GACATCTACT CGAGTCTCCA AAACCTCCCT GACGCTCAGC 
    CCCCCTGTCC CTGCTGAAGC ACCAGCATTT ACTCTTCCTC CTCGGAATAT CCGAGTGCAG 
    CTGGGAGCCA CAGCAAGATT TGAGGGGAAG GTCAGAGGCT ACCCCGAGCC TCAGATCACG 
    TGGTACAGGA ATGGGCATCC TGTCCCAGAG GGAGACCATT ATATCGTGGA CCGCAGCATT 
    CGAGGGATAT TCAGCTTGGT AATCAAAGGT GTACAGGAAA GTGATGGTGG CAAATACACC 
    TGCGAGGCTG CAAATGATGG TGGGGTTCGT CAAGTGACAG TGGAACTGAC AGTGGAAGGA 
    AACTCCTTGA AGAAATACAG CCTGCCATCC AGTGCCAAAA CCCCTGGGGG AAGACTTGCT 
    GTTCCATCTG TGGAGAATAG GCCAAGCATC TGGGGAGAGA GTCCACCCAA GTTTGCTACA 
    AAACCCAATA GAGTTGTTGT GCGTGAAGGC CAAACAGGCA AATTCTCATG TAAAATAACA 
    GGACGACCTC AGCCTCAAGT AACTTGGTTT AAGGGTGATG TTCAGCTGCA GCAAAATGAG 
    CGTTTCAACA TGTTTGAGAA AACAGGCATC CAGTTCTTGG AAATCCAAAA TGCTCAGCTC 
    ACTGATTCAG GAATTTACAC GTGCACAGTG GTAAACAGCG CTGGGAAGGC ATCTGTGTCT 
    GCAGAGCTCA CCGTTCAAGG TCCAGATAAG ACTGAGCCAT ACACTCAGCC ACCATGCATG 
    CCATCAAAAC CAACAACACT TACCATCAAA GCTGTTGAAA ATTCAGAATT CAAACAGGCA 
    ACTAGCAATG GGATTGCTAA AGAGCTGAAA TCCAACAACA CTGAATTCAT GGTTGAAACC 
    AAAGACAGGG TGTCAGCAAA AAAAGAGACC TTTTACTTCT CCAGAGAAGC CAAAGACATT 
    AAGCAGGGAC AGCACCAAGA ATCCAATGCA GTGTCTCCTC AAGAACCTAA AGAAGGAAAA 
    AAGGGATCCC AGGTTTTGCA GAAAACTTCA AGCACCATCA CACTACAAGC TGTCAGACTG 
    CAACCAGAAC CAAAGGCAGA ACCCCAGGCC ACTTTCATCG GACCAGGTGA AGACAGGAAA 
    AGGTCTGTGC AGCCCCTGCT GTCCACTCAG CAAACCCCAA CTCTCACAGG GCAAGTCAGT 
    CCAAGAAGTA GAGAAGCAGA AAACAGATCT GGAGCCCGGA AGGCTGTAAT GGAGGAGAAG 
    AGGGAGCCTC TAGGAATTCC CCCTCAGTTT GAGAGCCGTC CACAGAGCCT GGAAGCATCA 
    GAAGGCCAGG AGATTAAATT CAAGAGCAAA GTTTCAGGGA AACCAAAACC AGATGTGGAG 
    TGGTTCAAAG AGGGTGAACC TATTAAAGCT GGAGAAGATG TCCAAATATA TGAAGAAGAT 
    GGAATTCATT GCCTATGGTT AAAGAAGCCC TCGTTGGGGG ACAGTGGATC ATACACCTGT 
    ACAGCTTTCA ACCCCAAAGG CCAGACTTCC ACCAGCTGGT TGTTAACTGT CAGAAGGCCT 
    AAGGTTCAAG AGGTTGCTCC GTGCTTTTCC AGTGTCTTGA AAGGATGCAC CGTGAGTGAA 
    GGGCAAGACT TTGTGCTGCA GTGCTGTGTA GGAGGAGAAC CTGTACCTCA GATCACATGG 
    CTTCTCAACG ATCAGCCAAT TCAGTATGCC CATTCCACTT TTGAAGATGG AGTTGCAAAA 
    TTGACTGTCC AAGATGCCCT ACCAGAAGAT GATGGTATTT ATACCTGTCT GGCTGAAAAC 
    AACACGGGAC AGGCATCTTG CAGTGCTCGG GTCACAGTCA AAGAAAAGAG CAGCAAGAAG 
    GCAGAAAGTG CACAGACTGC CAAGTTAAAC AAGACTTTTG CACCCATCTT CCTTAAAGGC 
    CTGACTGACC TCAAAGTAAT GGATGGAAGT CAAGTGATAA TGACTGTCGA GGTATCAGCT 
    AACCCACCTC CTGAAATTAT TTGGCTGCAC AATGGGAAAG AAATCCAGGA GACAGAGGAT 
    TTCCACTTTG AGAAGAAAGG TAGTGAGTAC AGTCTGTACA TCCAGGAAGT ATTTCCTGAA 
    GACACAGGCA AATACACCTG TGAAGCCTGG AATGAATTAG GAGAAACTCA AACGCAGGCT 
    ACATTGACAG TACAAGAACC TCAGGATGGT ATTCAGCCCT GGTTCATTAG CAAACCGAGA 
    TCAGTGATAG CAGCTCCTGG GCAACCTGTC CTTATATCCT GTGCCATTGC TGGAGATCCT 
    TTCCCCACTG TTCACTGGTT TAAAGATGGG CAAGAAATAA TGCCAGGAGC AGCATGTGAA 
    ATCCTGCAAA ATGAAGACAT CTTCACACTG ATCTTGAGGA ATGTGCAGTC TCATCATGCA 
    GGGCAATATG AAATCCAGCT CAGGAACAAA GTTGGAGAAT GCAGCTGCCA GGTGTCTCTG 
    ATGCTGTGGA AGAGTTCAGC TTCCAGAGCA GAAATGCTCA GAGATGGGAG GGAATCAGCC 
    AGCCCCGGAG AGCGAAGAGA TGGTGGAGAT CATGGTAAAC TAACATTTGG TAGAACAAGT 
    GGCTTCAGAA AGAATAGCAG CGAAACCAGA GCAGCTGAGG AAGAGCAGGA AGACGTCCGA 
    GGCGTGCTGA AGAGGCGAGT GGAAACTCGG GAACACACAG AGGAGAGCCT GAGGCAGCAG 
    GAAGCTGAGC AACTTGATTT CCGTGACATT TTAGGCAAGA AAGTCAGCAC CAAAAGTTTT 
    TCTGAGGAGG AACTGAAAGA AATCCCAGCC GAGCAAATGG ACTTCCGAGC AAACCTGCAG 
    CGGCAGGTCA AACCCAAGAC CTTGTCAGAG GAGGAAAGGA AAGTTCATGC TCCTCAGCAG 
    GTGGACTTCC GTTCTGTGCT GGCCAAGAAG GGCACCCCAA AAACCCCATT GCCAGAGAAG 
    GCACCACCTC CTAAACCAGC TATTACAGAT TTCAGATCTG TGCTGGGTTC AAAGAAAAAG 
    CCACCTGCAG AGAATGGCAG CGCCAGCACC CCAGCACCAA ATGCCCGCAC CAATTCTGAA 
    GCCCAGAATG CGACCCCCAA TTCTCAAGCC CCTGTTGCCA AACCAGCAGT GAAAAAAGAA 
    GAGAAGAATG ACAGAAACTG TGAACATGGG TGCGCAGTTG TGGATGGTGG AATAATTGGG 
    AAAAAAGCTG AAAACAAACC AGCAGCAAGC AAACCACCTG TAAGCAAAGG AACAGCACCC 
    TCCTCTACAG AGAAGGTTCA GGATGCTCAA GTAGTAGATG GCGGAATAAC TGGGAAAAAA 
    CCTGAAAACA AACCAGCAGC AAGCAAACCA CCTGTAAGCA AAGGAACAGC ACCCTCCTCT 
    ACAGAGAAGG TTCAGGATGC TCAAGTAGTA GATGGCGGAA TAACTGGGAA AAAACCTGAA 
    AACAAACCAG CAGCAAGCAA ACCACCTGTA AGCAAAGGAA CAGCACCCTC CTTTACAGAG 
    AAGCTTCAGG ATGCTCAAGT AGTAGACGGT GAAAAATTGG TCTTACAATG TCGGATTTCC 
    TCTGATCCCC CTGCAGCTGT CACCTGGACT CTAGACAGCA AAGCCATCAA ATCATCAAAG 
    TCCATTGTCA TCTCCCAAGA AGGGACACTG TGTTCACTTA CCATTGAGAA GGCCACACCT 
    GAAGATGGTG GAGAATACAA ATGCATAGCT GAGAACACAG CAGGAAAAGC TGAATGTGCT 
    TGCAAAGTGC TGGTAGAAGA TGCCTCATCC ACAAAGACCT CAAAGCCTAC TGATAAAAAG 
    ACTGATAAAA AGAAGCCTAA GACCACACTT CCCCCTGTGC TGCCAACAGA GAGTGAAGCA 
    ACAGTGAAGA AGAAACCAGC TCCAAAGACT CCTCCAAAAG CAGCTGCTCC TCCTCAGATT 
    ACTCAGTTCC CAGAAGACAG AAAAGTCCGT GCGGGTGAAT CAGTGGAACT GTTTGCCAAG 
    GTGGTAGGAA CAGCACCCAT AACTTGCACC TGGATGAAAT TTCGTAAGCA GATTGAAGAA 
    AATGAATATA TTAAAATTGA GAATGCTGAA AACAGCAGCA AGCTCACAAT ATCATCAACA 
    AAGCAGGAAC ACTGTGGATG TTACACCCTC GTTGTAGAGA ACAAACTTGG CAGCAGACAA 
    GCACAAGTCA ACCTTACAGT TGTTGATAAA CCAGACCCAC CTGCTGGAAC ACCTTGCGCA 
    TCAGACATCC GGAGTTCCTC CCTCACTCTC TCATGGTACG GTTCCTCTTA CGATGGTGGA 
    AGTGCTGTGC AGTCCTACAC CGTGGAGATC TGGAACTCAG TGGATAACAA ATGGACAGAC 
    CTCACTACCT GCCGCAGCAC CTCTTTCAAC GTGCAGGACC TGCAGGCTGA CCGGGAGTAC 
    AAATTCCGCG TGCGAGCTGC GAACGTGTAC GGCATCAGCG AGCCCAGTCA AGAATCGGAG 
    CTGGTAAAAG TTGGAGAGAA ACAAGAAGAA CTTAAAGAGG ACGAAGCGGA GCTATCTGAC 
    GATGAAGGGA AAGAAACAGA GATCGAATAT CGGACGGTTA CTATCAACAC TGAACAAAAA 
    GTTTCAGATG TCTATAATAT CGAAGAAAGA CTTGGATCGG GGAAATTTGG ACAAGTCTTT 
    AGGCTTGTTG AAAAGAAGAA TGGAAAAGTT TGGGCAGGAA AATTTTTCAA AGCATATTCT 
    GCTAAGGAAA AAGAAAATAT AAGGGAGGAA ATCAGCATCA TGAACTGTCT ACATCATCCA 
    AAACTTGTCC AATGTGTAGA TGCCTTTGAA GAAAAAGCCA ACATCGTTAT GGTCTTGGAA 
    ATGGTTTCTG GAGGAGAACT CTTTGAACGA ATCATTGATG AAGACTTTGA ACTGACAGAG 
    AGAGAGTGCA TTAAATATAT GAAGCAGATT TCAGAAGGAG TTCAGTACAT CCATAAGCAG 
    GGAATTGTCC ACTTGGATTT GAAGCCAGAA AATATTATGT GTGTCAACAA GACCGGTACA 
    AGTATTAAGC TCATTGACTT TGGACTTGCA AGAAGATTAG AAAATGCAGG TTCTCTGAAA 
    GTTCTCTTTG GGACACCTGA GTTTGTGGCT CCAGAAGTTA TAAACTATGA ACCTATTGGA 
    TATGAAACAG ATATGTGGAG TATAGGAGTT ATCTGCTACA TTTTGGTCAG CGGACTTTCT 
    CCTTTCATGG GAGACAACGA CAACGAGACG CTCGCCAATG TCACCTCAGC AACGTGGGAC 
    TTCGACGATG AGGCTTTTGA TGAAATCTCC GATGATGCCA AGGACTTTAT CAGCAATCTC 
    CTCAAGAAAG ACATGAAGAG TCGCTTAAAT TGTACTCAAT GTCTTCAGCA TCCTTGGCTG 
    CAAAAAGACA CCAAAAACAT GGAAGCCAAA AAACTTTCCA AAGATAGGAT GAAGAAATAT 
    ATGGCCAGAA GAAAATGGCA GAAAACAGGC CATGCTGTCC GAGCAATAGG AAGGCTGTCG 
    TCCATGGCAA TGATTTCTGG TATGAGTGGG AGGAAGGCCT CTGGGAGCTC GCCTACCAGC 
    CCTATAAATG CAGACAAAGT AGAGAATGAA GATGCTTTCC TTGAAGAAGT GGCTGAAGAA 
    AAGCCCCATG TAAAGCCATA TTTTACTAAA AAAATCCTTG ACATAGAGGT TGTGGAAGGA 
    AGTGCTGCTA GATTTGACTG CAAAATTGAA GGCTATCCTG ACCCTGAGGT GATGTGGTAC 
    AAAGATGATC AGCCTGTCAA GGAGTCCCGT CACTTCCAGA TAGATTACGA TGAGGAGGGG 
    AACTGTTCTT TGACTATTGC TGAAGTTTGT GGGGACGATG ATGCCAAATA CACCTGCAAA 
    GCTGTTAACA GCCTCGGGGA AGCCACGTGC ACCGCAGAAC TCCTTGTGGA AACGATGGAA 
    GGAAAAGAAG AAGAAGAAGA TGAGGAGGAG GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014795316.1
    CDS83..6001
    Translation

    Target ORF information:

    RefSeq Version XM_014939830.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014939830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGGATG TGAAACTGGT GACATCTACT CGAGTCTCCA AAACCTCCCT GACGCTCAGC 
    CCCCCTGTCC CTGCTGAAGC ACCAGCATTT ACTCTTCCTC CTCGGAATAT CCGAGTGCAG 
    CTGGGAGCCA CAGCAAGATT TGAGGGGAAG GTCAGAGGCT ACCCCGAGCC TCAGATCACG 
    TGGTACAGGA ATGGGCATCC TGTCCCAGAG GGAGACCATT ATATCGTGGA CCGCAGCATT 
    CGAGGGATAT TCAGCTTGGT AATCAAAGGT GTACAGGAAA GTGATGGTGG CAAATACACC 
    TGCGAGGCTG CAAATGATGG TGGGGTTCGT CAAGTGACAG TGGAACTGAC AGTGGAAGGA 
    AACTCCTTGA AGAAATACAG CCTGCCATCC AGTGCCAAAA CCCCTGGGGG AAGACTTGCT 
    GTTCCATCTG TGGAGAATAG GCCAAGCATC TGGGGAGAGA GTCCACCCAA GTTTGCTACA 
    AAACCCAATA GAGTTGTTGT GCGTGAAGGC CAAACAGGCA AATTCTCATG TAAAATAACA 
    GGACGACCTC AGCCTCAAGT AACTTGGTTT AAGGGTGATG TTCAGCTGCA GCAAAATGAG 
    CGTTTCAACA TGTTTGAGAA AACAGGCATC CAGTTCTTGG AAATCCAAAA TGCTCAGCTC 
    ACTGATTCAG GAATTTACAC GTGCACAGTG GTAAACAGCG CTGGGAAGGC ATCTGTGTCT 
    GCAGAGCTCA CCGTTCAAGG TCCAGATAAG ACTGAGCCAT ACACTCAGCC ACCATGCATG 
    CCATCAAAAC CAACAACACT TACCATCAAA GCTGTTGAAA ATTCAGAATT CAAACAGGCA 
    ACTAGCAATG GGATTGCTAA AGAGCTGAAA TCCAACAACA CTGAATTCAT GGTTGAAACC 
    AAAGACAGGG TGTCAGCAAA AAAAGAGACC TTTTACTTCT CCAGAGAAGC CAAAGACATT 
    AAGCAGGGAC AGCACCAAGA ATCCAATGCA GTGTCTCCTC AAGAACCTAA AGAAGGAAAA 
    AAGGGATCCC AGGTTTTGCA GAAAACTTCA AGCACCATCA CACTACAAGC TGTCAGACTG 
    CAACCAGAAC CAAAGGCAGA ACCCCAGGCC ACTTTCATCG GACCAGGTGA AGACAGGAAA 
    AGGTCTGTGC AGCCCCTGCT GTCCACTCAG CAAACCCCAA CTCTCACAGG GCAAGTCAGT 
    CCAAGAAGTA GAGAAGCAGA AAACAGATCT GGAGCCCGGA AGGCTGTAAT GGAGGAGAAG 
    AGGGAGCCTC TAGGAATTCC CCCTCAGTTT GAGAGCCGTC CACAGAGCCT GGAAGCATCA 
    GAAGGCCAGG AGATTAAATT CAAGAGCAAA GTTTCAGGGA AACCAAAACC AGATGTGGAG 
    TGGTTCAAAG AGGGTGAACC TATTAAAGCT GGAGAAGATG TCCAAATATA TGAAGAAGAT 
    GGAATTCATT GCCTATGGTT AAAGAAGCCC TCGTTGGGGG ACAGTGGATC ATACACCTGT 
    ACAGCTTTCA ACCCCAAAGG CCAGACTTCC ACCAGCTGGT TGTTAACTGT CAGAAGGCCT 
    AAGGTTCAAG AGGTTGCTCC GTGCTTTTCC AGTGTCTTGA AAGGATGCAC CGTGAGTGAA 
    GGGCAAGACT TTGTGCTGCA GTGCTGTGTA GGAGGAGAAC CTGTACCTCA GATCACATGG 
    CTTCTCAACG ATCAGCCAAT TCAGTATGCC CATTCCACTT TTGAAGATGG AGTTGCAAAA 
    TTGACTGTCC AAGATGCCCT ACCAGAAGAT GATGGTATTT ATACCTGTCT GGCTGAAAAC 
    AACACGGGAC AGGCATCTTG CAGTGCTCGG GTCACAGTCA AAGAAAAGAG CAGCAAGAAG 
    GCAGAAAGTG CACAGACTGC CAAGTTAAAC AAGACTTTTG CACCCATCTT CCTTAAAGGC 
    CTGACTGACC TCAAAGTAAT GGATGGAAGT CAAGTGATAA TGACTGTCGA GGTATCAGCT 
    AACCCACCTC CTGAAATTAT TTGGCTGCAC AATGGGAAAG AAATCCAGGA GACAGAGGAT 
    TTCCACTTTG AGAAGAAAGG TAGTGAGTAC AGTCTGTACA TCCAGGAAGT ATTTCCTGAA 
    GACACAGGCA AATACACCTG TGAAGCCTGG AATGAATTAG GAGAAACTCA AACGCAGGCT 
    ACATTGACAG TACAAGAACC TCAGGATGGT ATTCAGCCCT GGTTCATTAG CAAACCGAGA 
    TCAGTGATAG CAGCTCCTGG GCAACCTGTC CTTATATCCT GTGCCATTGC TGGAGATCCT 
    TTCCCCACTG TTCACTGGTT TAAAGATGGG CAAGAAATAA TGCCAGGAGC AGCATGTGAA 
    ATCCTGCAAA ATGAAGACAT CTTCACACTG ATCTTGAGGA ATGTGCAGTC TCATCATGCA 
    GGGCAATATG AAATCCAGCT CAGGAACAAA GTTGGAGAAT GCAGCTGCCA GGTGTCTCTG 
    ATGCTGTGGA AGAGTTCAGC TTCCAGAGCA GAAATGCTCA GAGATGGGAG GGAATCAGCC 
    AGCCCCGGAG AGCGAAGAGA TGGTGGAGAT CATGGTAAAC TAACATTTGG TAGAACAAGT 
    GGCTTCAGAA AGAATAGCAG CGAAACCAGA GCAGCTGAGG AAGAGCAGGA AGACGTCCGA 
    GGCGTGCTGA AGAGGCGAGT GGAAACTCGG GAACACACAG AGGAGAGCCT GAGGCAGCAG 
    GAAGCTGAGC AACTTGATTT CCGTGACATT TTAGGCAAGA AAGTCAGCAC CAAAAGTTTT 
    TCTGAGGAGG AACTGAAAGA AATCCCAGCC GAGCAAATGG ACTTCCGAGC AAACCTGCAG 
    CGGCAGGTCA AACCCAAGAC CTTGTCAGAG GAGGAAAGGA AAGTTCATGC TCCTCAGCAG 
    GTGGACTTCC GTTCTGTGCT GGCCAAGAAG GGCACCCCAA AAACCCCATT GCCAGAGAAG 
    GCACCACCTC CTAAACCAGC TATTACAGAT TTCAGATCTG TGCTGGGTTC AAAGAAAAAG 
    CCACCTGCAG AGAATGGCAG CGCCAGCACC CCAGCACCAA ATGCCCGCAC CAATTCTGAA 
    GCCCAGAATG CGACCCCCAA TTCTCAAGCC CCTGTTGCCA AACCAGCAGT GAAAAAAGAA 
    GAGAAGAATG ACAGAAACTG TGAACATGGG TGCGCAGTTG TGGATGGTGG AATAATTGGG 
    AAAAAAGCTG AAAACAAACC AGCAGCAAGC AAACCACCTG TAAGCAAAGG AACAGCACCC 
    TCCTCTACAG AGAAGGTTCA GGATGCTCAA GTAGTAGATG GCGGAATAAC TGGGAAAAAA 
    CCTGAAAACA AACCAGCAGC AAGCAAACCA CCTGTAAGCA AAGGAACAGC ACCCTCCTCT 
    ACAGAGAAGG TTCAGGATGC TCAAGTAGTA GATGGCGGAA TAACTGGGAA AAAACCTGAA 
    AACAAACCAG CAGCAAGCAA ACCACCTGTA AGCAAAGGAA CAGCACCCTC CTTTACAGAG 
    AAGCTTCAGG ATGCTCAAGT AGTAGACGGT GAAAAATTGG TCTTACAATG TCGGATTTCC 
    TCTGATCCCC CTGCAGCTGT CACCTGGACT CTAGACAGCA AAGCCATCAA ATCATCAAAG 
    TCCATTGTCA TCTCCCAAGA AGGGACACTG TGTTCACTTA CCATTGAGAA GGCCACACCT 
    GAAGATGGTG GAGAATACAA ATGCATAGCT GAGAACACAG CAGGAAAAGC TGAATGTGCT 
    TGCAAAGTGC TGGTAGAAGA TGCCTCATCC ACAAAGACCT CAAAGCCTAC TGATAAAAAG 
    ACTGATAAAA AGAAGCCTAA GACCACACTT CCCCCTGTGC TGCCAACAGA GAGTGAAGCA 
    ACAGTGAAGA AGAAACCAGC TCCAAAGACT CCTCCAAAAG CAGCTGCTCC TCCTCAGATT 
    ACTCAGTTCC CAGAAGACAG AAAAGTCCGT GCGGGTGAAT CAGTGGAACT GTTTGCCAAG 
    GTGGTAGGAA CAGCACCCAT AACTTGCACC TGGATGAAAT TTCGTAAGCA GATTGAAGAA 
    AATGAATATA TTAAAATTGA GAATGCTGAA AACAGCAGCA AGCTCACAAT ATCATCAACA 
    AAGCAGGAAC ACTGTGGATG TTACACCCTC GTTGTAGAGA ACAAACTTGG CAGCAGACAA 
    GCACAAGTCA ACCTTACAGT TGTTGATAAA CCAGACCCAC CTGCTGGAAC ACCTTGCGCA 
    TCAGACATCC GGAGTTCCTC CCTCACTCTC TCATGGTACG GTTCCTCTTA CGATGGTGGA 
    AGTGCTGTGC AGTCCTACAC CGTGGAGATC TGGAACTCAG TGGATAACAA ATGGACAGAC 
    CTCACTACCT GCCGCAGCAC CTCTTTCAAC GTGCAGGACC TGCAGGCTGA CCGGGAGTAC 
    AAATTCCGCG TGCGAGCTGC GAACGTGTAC GGCATCAGCG AGCCCAGTCA AGAATCGGAG 
    CTGGTAAAAG TTGGAGAGAA ACAAGAAGAA CTTAAAGAGG ACGAAGCGGA GCTATCTGAC 
    GATGAAGGGA AAGAAACAGA GATCGAATAT CGGACGGTTA CTATCAACAC TGAACAAAAA 
    GTTTCAGATG TCTATAATAT CGAAGAAAGA CTTGGATCGG GGAAATTTGG ACAAGTCTTT 
    AGGCTTGTTG AAAAGAAGAA TGGAAAAGTT TGGGCAGGAA AATTTTTCAA AGCATATTCT 
    GCTAAGGAAA AAGAAAATAT AAGGGAGGAA ATCAGCATCA TGAACTGTCT ACATCATCCA 
    AAACTTGTCC AATGTGTAGA TGCCTTTGAA GAAAAAGCCA ACATCGTTAT GGTCTTGGAA 
    ATGGTTTCTG GAGGAGAACT CTTTGAACGA ATCATTGATG AAGACTTTGA ACTGACAGAG 
    AGAGAGTGCA TTAAATATAT GAAGCAGATT TCAGAAGGAG TTCAGTACAT CCATAAGCAG 
    GGAATTGTCC ACTTGGATTT GAAGCCAGAA AATATTATGT GTGTCAACAA GACCGGTACA 
    AGTATTAAGC TCATTGACTT TGGACTTGCA AGAAGATTAG AAAATGCAGG TTCTCTGAAA 
    GTTCTCTTTG GGACACCTGA GTTTGTGGCT CCAGAAGTTA TAAACTATGA ACCTATTGGA 
    TATGAAACAG ATATGTGGAG TATAGGAGTT ATCTGCTACA TTTTGGTCAG CGGACTTTCT 
    CCTTTCATGG GAGACAACGA CAACGAGACG CTCGCCAATG TCACCTCAGC AACGTGGGAC 
    TTCGACGATG AGGCTTTTGA TGAAATCTCC GATGATGCCA AGGACTTTAT CAGCAATCTC 
    CTCAAGAAAG ACATGAAGAG TCGCTTAAAT TGTACTCAAT GTCTTCAGCA TCCTTGGCTG 
    CAAAAAGACA CCAAAAACAT GGAAGCCAAA AAACTTTCCA AAGATAGGAT GAAGAAATAT 
    ATGGCCAGAA GAAAATGGCA GAAAACAGGC CATGCTGTCC GAGCAATAGG AAGGCTGTCG 
    TCCATGGCAA TGATTTCTGG TATGAGTGGG AGGAAGGCCT CTGGGAGCTC GCCTACCAGC 
    CCTATAAATG CAGACAAAGT AGAGAATGAA GATGCTTTCC TTGAAGAAGT GGCTGAAGAA 
    AAGCCCCATG TAAAGCCATA TTTTACTAAA AAAATCCTTG ACATAGAGGT TGTGGAAGGA 
    AGTGCTGCTA GATTTGACTG CAAAATTGAA GGCTATCCTG ACCCTGAGGT GATGTGGTAC 
    AAAGATGATC AGCCTGTCAA GGAGTCCCGT CACTTCCAGA TAGATTACGA TGAGGAGGGG 
    AACTGTTCTT TGACTATTGC TGAAGTTTGT GGGGACGATG ATGCCAAATA CACCTGCAAA 
    GCTGTTAACA GCCTCGGGGA AGCCACGTGC ACCGCAGAAC TCCTTGTGGA AACGATGGAA 
    GGAAAAGAAG AAGAAGAAGA TGAGGAGGAG GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MYLK ( XM_014939837.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795323.1 Calidris pugnax myosin light chain kinase (MYLK), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa58138
    Clone ID Related Accession (Same CDS sequence) XM_014939837.1
    Accession Version XM_014939837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090770.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTTTCTG GAAAACCAGT TCATTTTGTT CCTGTCACTC TTCCATCTGG TGTGAGTGGG 
    ACCATAAAAT ATAACCAGGT GGAAGAAAGG CTTTCAGAAA AGAGCAGCAA GAAGGCAGAA 
    AGTGCACAGA CTGCCAAGTT AAACAAGACT TTTGCACCCA TCTTCCTTAA AGGCCTGACT 
    GACCTCAAAG TAATGGATGG AAGTCAAGTG ATAATGACTG TCGAGGTATC AGCTAACCCA 
    CCTCCTGAAA TTATTTGGCT GCACAATGGG AAAGAAATCC AGGAGACAGA GGATTTCCAC 
    TTTGAGAAGA AAGGTAGTGA GTACAGTCTG TACATCCAGG AAGTATTTCC TGAAGACACA 
    GGCAAATACA CCTGTGAAGC CTGGAATGAA TTAGGAGAAA CTCAAACGCA GGCTACATTG 
    ACAGTACAAG AACCTCAGGA TGGTATTCAG CCCTGGTTCA TTAGCAAACC GAGATCAGTG 
    ATAGCAGCTC CTGGGCAACC TGTCCTTATA TCCTGTGCCA TTGCTGGAGA TCCTTTCCCC 
    ACTGTTCACT GGTTTAAAGA TGGGCAAGAA ATAATGCCAG GAGCAGCATG TGAAATCCTG 
    CAAAATGAAG ACATCTTCAC ACTGATCTTG AGGAATGTGC AGTCTCATCA TGCAGGGCAA 
    TATGAAATCC AGCTCAGGAA CAAAGTTGGA GAATGCAGCT GCCAGGTGTC TCTGATGCTG 
    TGGAAGAGTT CAGCTTCCAG AGCAGAAATG CTCAGAGATG GGAGGGAATC AGCCAGCCCC 
    GGAGAGCGAA GAGATGGTGG AGATCATGGT AAACTAACAT TTGGTAGAAC AAGTGGCTTC 
    AGAAAGAATA GCAGCGAAAC CAGAGCAGCT GAGGAAGAGC AGGAAGACGT CCGAGGCGTG 
    CTGAAGAGGC GAGTGGAAAC TCGGGAACAC ACAGAGGAGA GCCTGAGGCA GCAGGAAGCT 
    GAGCAACTTG ATTTCCGTGA CATTTTAGGC AAGAAAGTCA GCACCAAAAG TTTTTCTGAG 
    GAGGAACTGA AAGAAATCCC AGCCGAGCAA ATGGACTTCC GAGCAAACCT GCAGCGGCAG 
    GTCAAACCCA AGACCTTGTC AGAGGAGGAA AGGAAAGTTC ATGCTCCTCA GCAGGTGGAC 
    TTCCGTTCTG TGCTGGCCAA GAAGGGCACC CCAAAAACCC CATTGCCAGA GAAGGCACCA 
    CCTCCTAAAC CAGCTATTAC AGATTTCAGA TCTGTGCTGG GTTCAAAGAA AAAGCCACCT 
    GCAGAGAATG GCAGCGCCAG CACCCCAGCA CCAAATGCCC GCACCAATTC TGAAGCCCAG 
    AATGCGACCC CCAATTCTCA AGCCCCTGTT GCCAAACCAG CAGTGAAAAA AGAAGAGAAG 
    AATGACAGAA ACTGTGAACA TGGGTGCGCA GTTGTGGATG GTGGAATAAT TGGGAAAAAA 
    GCTGAAAACA AACCAGCAGC AAGCAAACCA CCTGTAAGCA AAGGAACAGC ACCCTCCTCT 
    ACAGAGAAGG TTCAGGATGC TCAAGTAGTA GATGGCGGAA TAACTGGGAA AAAACCTGAA 
    AACAAACCAG CAGCAAGCAA ACCACCTGTA AGCAAAGGAA CAGCACCCTC CTCTACAGAG 
    AAGGTTCAGG ATGCTCAAGT AGTAGATGGC GGAATAACTG GGAAAAAACC TGAAAACAAA 
    CCAGCAGCAA GCAAACCACC TGTAAGCAAA GGAACAGCAC CCTCCTTTAC AGAGAAGCTT 
    CAGGATGCTC AAGTAGTAGA CGGTGAAAAA TTGGTCTTAC AATGTCGGAT TTCCTCTGAT 
    CCCCCTGCAG CTGTCACCTG GACTCTAGAC AGCAAAGCCA TCAAATCATC AAAGTCCATT 
    GTCATCTCCC AAGAAGGGAC ACTGTGTTCA CTTACCATTG AGAAGGCCAC ACCTGAAGAT 
    GGTGGAGAAT ACAAATGCAT AGCTGAGAAC ACAGCAGGAA AAGCTGAATG TGCTTGCAAA 
    GTGCTGGTAG AAGATGCCTC ATCCACAAAG ACCTCAAAGC CTACTGATAA AAAGACTGAT 
    AAAAAGAAGC CTAAGACCAC ACTTCCCCCT GTGCTGCCAA CAGAGAGTGA AGCAACAGTG 
    AAGAAGAAAC CAGCTCCAAA GACTCCTCCA AAAGCAGCTG CTCCTCCTCA GATTACTCAG 
    TTCCCAGAAG ACAGAAAAGT CCGTGCGGGT GAATCAGTGG AACTGTTTGC CAAGGTGGTA 
    GGAACAGCAC CCATAACTTG CACCTGGATG AAATTTCGTA AGCAGATTGA AGAAAATGAA 
    TATATTAAAA TTGAGAATGC TGAAAACAGC AGCAAGCTCA CAATATCATC AACAAAGCAG 
    GAACACTGTG GATGTTACAC CCTCGTTGTA GAGAACAAAC TTGGCAGCAG ACAAGCACAA 
    GTCAACCTTA CAGTTGTTGA TAAACCAGAC CCACCTGCTG GAACACCTTG CGCATCAGAC 
    ATCCGGAGTT CCTCCCTCAC TCTCTCATGG TACGGTTCCT CTTACGATGG TGGAAGTGCT 
    GTGCAGTCCT ACACCGTGGA GATCTGGAAC TCAGTGGATA ACAAATGGAC AGACCTCACT 
    ACCTGCCGCA GCACCTCTTT CAACGTGCAG GACCTGCAGG CTGACCGGGA GTACAAATTC 
    CGCGTGCGAG CTGCGAACGT GTACGGCATC AGCGAGCCCA GTCAAGAATC GGAGCTGGTA 
    AAAGTTGGAG AGAAACAAGA AGAACTTAAA GAGGACGAAG CGGAGCTATC TGACGATGAA 
    GGGAAAGAAA CAGAGATCGA ATATCGGACG GTTACTATCA ACACTGAACA AAAAGTTTCA 
    GATGTCTATA ATATCGAAGA AAGACTTGGA TCGGGGAAAT TTGGACAAGT CTTTAGGCTT 
    GTTGAAAAGA AGAATGGAAA AGTTTGGGCA GGAAAATTTT TCAAAGCATA TTCTGCTAAG 
    GAAAAAGAAA ATATAAGGGA GGAAATCAGC ATCATGAACT GTCTACATCA TCCAAAACTT 
    GTCCAATGTG TAGATGCCTT TGAAGAAAAA GCCAACATCG TTATGGTCTT GGAAATGGTT 
    TCTGGAGGAG AACTCTTTGA ACGAATCATT GATGAAGACT TTGAACTGAC AGAGAGAGAG 
    TGCATTAAAT ATATGAAGCA GATTTCAGAA GGAGTTCAGT ACATCCATAA GCAGGGAATT 
    GTCCACTTGG ATTTGAAGCC AGAAAATATT ATGTGTGTCA ACAAGACCGG TACAAGTATT 
    AAGCTCATTG ACTTTGGACT TGCAAGAAGA TTAGAAAATG CAGGTTCTCT GAAAGTTCTC 
    TTTGGGACAC CTGAGTTTGT GGCTCCAGAA GTTATAAACT ATGAACCTAT TGGATATGAA 
    ACAGATATGT GGAGTATAGG AGTTATCTGC TACATTTTGG TCAGCGGACT TTCTCCTTTC 
    ATGGGAGACA ACGACAACGA GACGCTCGCC AATGTCACCT CAGCAACGTG GGACTTCGAC 
    GATGAGGCTT TTGATGAAAT CTCCGATGAT GCCAAGGACT TTATCAGCAA TCTCCTCAAG 
    AAAGACATGA AGAGTCGCTT AAATTGTACT CAATGTCTTC AGCATCCTTG GCTGCAAAAA 
    GACACCAAAA ACATGGAAGC CAAAAAACTT TCCAAAGATA GGATGAAGAA ATATATGGCC 
    AGAAGAAAAT GGCAGAAAAC AGGCCATGCT GTCCGAGCAA TAGGAAGGCT GTCGTCCATG 
    GCAATGATTT CTGGTATGAG TGGGAGGAAG GCCTCTGGGA GCTCGCCTAC CAGCCCTATA 
    AATGCAGACA AAGTAGAGAA TGAAGATGCT TTCCTTGAAG AAGTGGCTGA AGAAAAGCCC 
    CATGTAAAGC CATATTTTAC TAAAAAAATC CTTGACATAG AGGTTGTGGA AGGAAGTGCT 
    GCTAGATTTG ACTGCAAAAT TGAAGGCTAT CCTGACCCTG AGGTGATGTG GTACAAAGAT 
    GATCAGCCTG TCAAGGAGTC CCGTCACTTC CAGATAGATT ACGATGAGGA GGGGAACTGT 
    TCTTTGACTA TTGCTGAAGT TTGTGGGGAC GATGATGCCA AATACACCTG CAAAGCTGTT 
    AACAGCCTCG GGGAAGCCAC GTGCACCGCA GAACTCCTTG TGGAAACGAT GGAAGGAAAA 
    GAAGAAGAAG AAGATGAGGA GGAGGAAGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014795323.1
    CDS7..4179
    Translation

    Target ORF information:

    RefSeq Version XM_014939837.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014939837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTTTCTG GAAAACCAGT TCATTTTGTT CCTGTCACTC TTCCATCTGG TGTGAGTGGG 
    ACCATAAAAT ATAACCAGGT GGAAGAAAGG CTTTCAGAAA AGAGCAGCAA GAAGGCAGAA 
    AGTGCACAGA CTGCCAAGTT AAACAAGACT TTTGCACCCA TCTTCCTTAA AGGCCTGACT 
    GACCTCAAAG TAATGGATGG AAGTCAAGTG ATAATGACTG TCGAGGTATC AGCTAACCCA 
    CCTCCTGAAA TTATTTGGCT GCACAATGGG AAAGAAATCC AGGAGACAGA GGATTTCCAC 
    TTTGAGAAGA AAGGTAGTGA GTACAGTCTG TACATCCAGG AAGTATTTCC TGAAGACACA 
    GGCAAATACA CCTGTGAAGC CTGGAATGAA TTAGGAGAAA CTCAAACGCA GGCTACATTG 
    ACAGTACAAG AACCTCAGGA TGGTATTCAG CCCTGGTTCA TTAGCAAACC GAGATCAGTG 
    ATAGCAGCTC CTGGGCAACC TGTCCTTATA TCCTGTGCCA TTGCTGGAGA TCCTTTCCCC 
    ACTGTTCACT GGTTTAAAGA TGGGCAAGAA ATAATGCCAG GAGCAGCATG TGAAATCCTG 
    CAAAATGAAG ACATCTTCAC ACTGATCTTG AGGAATGTGC AGTCTCATCA TGCAGGGCAA 
    TATGAAATCC AGCTCAGGAA CAAAGTTGGA GAATGCAGCT GCCAGGTGTC TCTGATGCTG 
    TGGAAGAGTT CAGCTTCCAG AGCAGAAATG CTCAGAGATG GGAGGGAATC AGCCAGCCCC 
    GGAGAGCGAA GAGATGGTGG AGATCATGGT AAACTAACAT TTGGTAGAAC AAGTGGCTTC 
    AGAAAGAATA GCAGCGAAAC CAGAGCAGCT GAGGAAGAGC AGGAAGACGT CCGAGGCGTG 
    CTGAAGAGGC GAGTGGAAAC TCGGGAACAC ACAGAGGAGA GCCTGAGGCA GCAGGAAGCT 
    GAGCAACTTG ATTTCCGTGA CATTTTAGGC AAGAAAGTCA GCACCAAAAG TTTTTCTGAG 
    GAGGAACTGA AAGAAATCCC AGCCGAGCAA ATGGACTTCC GAGCAAACCT GCAGCGGCAG 
    GTCAAACCCA AGACCTTGTC AGAGGAGGAA AGGAAAGTTC ATGCTCCTCA GCAGGTGGAC 
    TTCCGTTCTG TGCTGGCCAA GAAGGGCACC CCAAAAACCC CATTGCCAGA GAAGGCACCA 
    CCTCCTAAAC CAGCTATTAC AGATTTCAGA TCTGTGCTGG GTTCAAAGAA AAAGCCACCT 
    GCAGAGAATG GCAGCGCCAG CACCCCAGCA CCAAATGCCC GCACCAATTC TGAAGCCCAG 
    AATGCGACCC CCAATTCTCA AGCCCCTGTT GCCAAACCAG CAGTGAAAAA AGAAGAGAAG 
    AATGACAGAA ACTGTGAACA TGGGTGCGCA GTTGTGGATG GTGGAATAAT TGGGAAAAAA 
    GCTGAAAACA AACCAGCAGC AAGCAAACCA CCTGTAAGCA AAGGAACAGC ACCCTCCTCT 
    ACAGAGAAGG TTCAGGATGC TCAAGTAGTA GATGGCGGAA TAACTGGGAA AAAACCTGAA 
    AACAAACCAG CAGCAAGCAA ACCACCTGTA AGCAAAGGAA CAGCACCCTC CTCTACAGAG 
    AAGGTTCAGG ATGCTCAAGT AGTAGATGGC GGAATAACTG GGAAAAAACC TGAAAACAAA 
    CCAGCAGCAA GCAAACCACC TGTAAGCAAA GGAACAGCAC CCTCCTTTAC AGAGAAGCTT 
    CAGGATGCTC AAGTAGTAGA CGGTGAAAAA TTGGTCTTAC AATGTCGGAT TTCCTCTGAT 
    CCCCCTGCAG CTGTCACCTG GACTCTAGAC AGCAAAGCCA TCAAATCATC AAAGTCCATT 
    GTCATCTCCC AAGAAGGGAC ACTGTGTTCA CTTACCATTG AGAAGGCCAC ACCTGAAGAT 
    GGTGGAGAAT ACAAATGCAT AGCTGAGAAC ACAGCAGGAA AAGCTGAATG TGCTTGCAAA 
    GTGCTGGTAG AAGATGCCTC ATCCACAAAG ACCTCAAAGC CTACTGATAA AAAGACTGAT 
    AAAAAGAAGC CTAAGACCAC ACTTCCCCCT GTGCTGCCAA CAGAGAGTGA AGCAACAGTG 
    AAGAAGAAAC CAGCTCCAAA GACTCCTCCA AAAGCAGCTG CTCCTCCTCA GATTACTCAG 
    TTCCCAGAAG ACAGAAAAGT CCGTGCGGGT GAATCAGTGG AACTGTTTGC CAAGGTGGTA 
    GGAACAGCAC CCATAACTTG CACCTGGATG AAATTTCGTA AGCAGATTGA AGAAAATGAA 
    TATATTAAAA TTGAGAATGC TGAAAACAGC AGCAAGCTCA CAATATCATC AACAAAGCAG 
    GAACACTGTG GATGTTACAC CCTCGTTGTA GAGAACAAAC TTGGCAGCAG ACAAGCACAA 
    GTCAACCTTA CAGTTGTTGA TAAACCAGAC CCACCTGCTG GAACACCTTG CGCATCAGAC 
    ATCCGGAGTT CCTCCCTCAC TCTCTCATGG TACGGTTCCT CTTACGATGG TGGAAGTGCT 
    GTGCAGTCCT ACACCGTGGA GATCTGGAAC TCAGTGGATA ACAAATGGAC AGACCTCACT 
    ACCTGCCGCA GCACCTCTTT CAACGTGCAG GACCTGCAGG CTGACCGGGA GTACAAATTC 
    CGCGTGCGAG CTGCGAACGT GTACGGCATC AGCGAGCCCA GTCAAGAATC GGAGCTGGTA 
    AAAGTTGGAG AGAAACAAGA AGAACTTAAA GAGGACGAAG CGGAGCTATC TGACGATGAA 
    GGGAAAGAAA CAGAGATCGA ATATCGGACG GTTACTATCA ACACTGAACA AAAAGTTTCA 
    GATGTCTATA ATATCGAAGA AAGACTTGGA TCGGGGAAAT TTGGACAAGT CTTTAGGCTT 
    GTTGAAAAGA AGAATGGAAA AGTTTGGGCA GGAAAATTTT TCAAAGCATA TTCTGCTAAG 
    GAAAAAGAAA ATATAAGGGA GGAAATCAGC ATCATGAACT GTCTACATCA TCCAAAACTT 
    GTCCAATGTG TAGATGCCTT TGAAGAAAAA GCCAACATCG TTATGGTCTT GGAAATGGTT 
    TCTGGAGGAG AACTCTTTGA ACGAATCATT GATGAAGACT TTGAACTGAC AGAGAGAGAG 
    TGCATTAAAT ATATGAAGCA GATTTCAGAA GGAGTTCAGT ACATCCATAA GCAGGGAATT 
    GTCCACTTGG ATTTGAAGCC AGAAAATATT ATGTGTGTCA ACAAGACCGG TACAAGTATT 
    AAGCTCATTG ACTTTGGACT TGCAAGAAGA TTAGAAAATG CAGGTTCTCT GAAAGTTCTC 
    TTTGGGACAC CTGAGTTTGT GGCTCCAGAA GTTATAAACT ATGAACCTAT TGGATATGAA 
    ACAGATATGT GGAGTATAGG AGTTATCTGC TACATTTTGG TCAGCGGACT TTCTCCTTTC 
    ATGGGAGACA ACGACAACGA GACGCTCGCC AATGTCACCT CAGCAACGTG GGACTTCGAC 
    GATGAGGCTT TTGATGAAAT CTCCGATGAT GCCAAGGACT TTATCAGCAA TCTCCTCAAG 
    AAAGACATGA AGAGTCGCTT AAATTGTACT CAATGTCTTC AGCATCCTTG GCTGCAAAAA 
    GACACCAAAA ACATGGAAGC CAAAAAACTT TCCAAAGATA GGATGAAGAA ATATATGGCC 
    AGAAGAAAAT GGCAGAAAAC AGGCCATGCT GTCCGAGCAA TAGGAAGGCT GTCGTCCATG 
    GCAATGATTT CTGGTATGAG TGGGAGGAAG GCCTCTGGGA GCTCGCCTAC CAGCCCTATA 
    AATGCAGACA AAGTAGAGAA TGAAGATGCT TTCCTTGAAG AAGTGGCTGA AGAAAAGCCC 
    CATGTAAAGC CATATTTTAC TAAAAAAATC CTTGACATAG AGGTTGTGGA AGGAAGTGCT 
    GCTAGATTTG ACTGCAAAAT TGAAGGCTAT CCTGACCCTG AGGTGATGTG GTACAAAGAT 
    GATCAGCCTG TCAAGGAGTC CCGTCACTTC CAGATAGATT ACGATGAGGA GGGGAACTGT 
    TCTTTGACTA TTGCTGAAGT TTGTGGGGAC GATGATGCCA AATACACCTG CAAAGCTGTT 
    AACAGCCTCG GGGAAGCCAC GTGCACCGCA GAACTCCTTG TGGAAACGAT GGAAGGAAAA 
    GAAGAAGAAG AAGATGAGGA GGAGGAAGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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