MTOR cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTOR
Entrez Gene ID	106887740
Full Name	mechanistic target of rapamycin kinase
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014941451.1	XP_014796937.1	serine/threonine-protein kinase mTOR

Pathways from BioSystems
Gene Ontology

The following MTOR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTOR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa64647	XM_014941451.1 Latest version!	Calidris pugnax mechanistic target of rapamycin (serine/threonine kinase) (MTOR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTOR ( XM_014941451.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796937.1 Calidris pugnax mechanistic target of rapamycin (serine/threonine kinase) (MTOR), mRNA.

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CloneID	OCa64647
Clone ID Related Accession (Same CDS sequence)	XM_014941451.1
Accession Version	XM_014941451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase mTOR
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA CCGTCTCCAT CCTCCAACAA TTTGCCAATG GCCTGAAGAG TCGCAGTGAG 
GAGACGAGGG CGAAAGCTGC CAAGGACTTG CAGCATTATG TCACCATGGA GCTCCGCGAG 
ATGAGTCAAG AAGAATCTAC CAGGTTTTAT GATCAGCTGA ACCATCACAT ATTTGAGCTG 
GTCTCTAGCT CTGATGCAAA TGAAAGGAAG GGTGGCATTC TGGCTATAGC AAGCCTTATT 
GGTGTTGAAG GAGGTAACGC CACCCGTATT GGCAGGTTTG CCAACTACTT GAGGAATCTC 
TTGCCATCCA ATGACCCTGT TGTAATGGAG ATGGCTTCCA AGGCTATTGG GCGGCTTGCG 
ATGGCTGGTG ACACCTTCAC AGCTGAGTAT GTGGAGTTTG AGGTGAAACG AGCTCTGGAA 
TGGCTGGGAG CTGACAGGAA TGAAGGTCGA AGACATGCTG CTGTTCTTGT CCTGCGTGAG 
CTGGCAATCA GCGTGCCTAC CTTCTTCTTC CAACAAGTGC AGCCATTCTT TGACAATATA 
TTTGTGGCTG TGTGGGATCC CAAACAGGCC ATCCGAGAAG GAGCTGTTTC TGCCTTAAGA 
GCCTGCCTTA TCCTCACCAC TCAACGGGAG CCAAAAGAGA TGCAGAAGCC CCAGTGGTAC 
AGACATACAT ATGAAGAGGC TGAGAAAGGC TTTGATGAGA CTTTGGCCAA AGAGAAAGGA 
ATGAACCGTG ATGACCGAAT CCATGGTGCC TTACTGATCC TCAATGAGCT AGTGAGAATA 
AGCAGTATGG AGGGAGAGCG CCTTAGAGAG GAGATGGAAG AGATCACTCA GCAGCAGCTT 
GTGCATGACA AGTACTGCAA GGACCTGATG GGCTTCAGTA CCAAACCTCG CCACATCACT 
CCCTTCACTA GCTTCCAGTC TCTTCAGCCC TCTCAGTCCA ATGCTTTGGC TGGTCTTCTG 
GGGTACAGTG CCCACCAAGG AATCATGGGT TTCGCAACCT CCCCTGTCCC AGCAAAGTCT 
ACATTGGTAG AAAGTCGATG CTGCAGGGAT CTAATGGAAG AGAAGTTTGA TCAAGTGTGC 
CAGTGGGTTC TCAAGTGCAG AACCAGCAAA AATTCTTTGA TCCAGATGAC GGTTCTCAAT 
CTGCTACCCC GGCTGGCGGC TTTCCGTCCT TCCGCGTTCA CAGCTGATCA GTATCTTCCA 
GACACCATGA ATCATGTTCT CAGCTGTGTG AAGAAGGAGA AGGAGCGAAC AGCAGCCTTC 
CAGGCCTTGG GCTTGCTTTC TGTGGCCGTG AGGTCTGAGT TTCAAGCCTA CCTGCCAAAA 
GTCCTTGAAA TCATAAAAGC AGCTCTGCCA CCAAAGGACT TTGCCCACAA GAGGCAGAAG 
TCCGTGCAGG TTGATGCCAC AGTCTTCACC TGCATTAGTA TGTTGGCACG AGCCATGGGA 
CCCAGCATCC AGCAGGACAT CAAAGAGCTT TTGGAGCCGA TGCTGGCTGT GGGGCTGAGC 
CCTGCTCTAA CAGCGGTGCT GTACGACTTG AGTCGCCAGA TCCCTCAGCT TAAGAAGGAT 
ATCCAGGATG GGCTGCTGAA AATGCTCTCC CTTGTTCTAA TGCACAAGCC CCTGCGACAT 
CCGGGCATGC CGAAGGGCCT TGCCCACCAG CTCGCCTCCC CGAGTCTCAC CAACATCCCA 
GAGGCCAGCG ACGTGGGCAG CATCACCCTT GCTCTTCGAA CGCTGGGCAG CTTTGAGTTT 
GAAGGCCACT CTCTGACTCA GTTTGTTCGG CACTGTGCAG ACCACTTTCT GAACAGCGAG 
CACAAGGAGA TCCGGATGGA AGCAGCCCGC ACCTGCTCTC GCTTGCTCAC ACCTTCCATC 
CACTTGATCA GTGGCCATGC CCACGTGGTC AGTCAGACAG CAGTGCAGGT CGTGGCTGAC 
GTTCTCAGCA AACTGCTTGT GGTTGGAATA ACCGACCCAG ACCCTGATAT ACGTTTCTGT 
GTGTTGGCTT CTCTGGATGA GCGATTTGAT GCTCACCTTG CCCAGGCGGA AAATTTGCAA 
GCTCTTTTTG TGGCCCTGAA TGATCAAGTA TTTGAAATCC GCGAGCTGGC CATCTGTACT 
GTGGGTCGCC TGAGCAGCAT GAACCCTGCT TTTGTCATGC CTTTCCTGCG CAAGATGCTA 
ATCCAGATTC TAACAGAGCT GGAACACAGT GGTGTTGGCC GGATTAAAGA GCAAAGTGCC 
AGGATGTTGG GTCACCTGGT CTCTAACGCT CCCCGCCTCA TTCGTCCGTA TATGGAGCCT 
ATTCTTAAGG CGCTAATTGT GAAACTGAAA GATCCTGATC CAGATCCAAA TCCAGGGGTG 
ATTAACAATG TCCTAGCTAC CATAGGGGAG CTTGCACAGG TCAGTGGGCT GGAGATGAGG 
AAGTGGGTGG ATGAACTCTT CATTATCATT ATGGACATGC TGCAGGACTC CTCCCTGCTG 
GCTAAAAGAC AAGTGGCTCT TTGGACACTG GGGCAGCTTG TGGCCAGCAC TGGTTATGTG 
GTGGAACCAT ACAGAAAGTA CCCAACTTTG CTGGAGGTGC TTCTGAATTT CCTGAAGACT 
GAGCAGAATC AGGGCACCCG TAGAGAGGCT ATTCGTGTGT TAGGGTTGCT GGGTGCTTTG 
GACCCTTACA AACACAAAGT GAACATTGGC ATGATTGATC AGTCACGAGA TGCTTCAGCT 
GTCAGCCTCT CAGAGTCCAA ATCCAGCCAG GATTCTTCGG ATTACAGTAC CAGTGAGATG 
CTGGTGAACA TGGGAAACCT CCCTTTGGAT GAGTTCTATC CAGCTGTCTC TATGGTGGCA 
CTGATGAGAA TTTTCCGAGA CCAGTCCTTG TCCCAACACC ACACTATGGT AGTTCAGGCC 
ATCACCTTTA TTTTCAAATC TCTTGGGCTG AAGTGTGTGC AGTTCCTGCC CCAGGTCATG 
CCAACGTTCC TTAATGTAAT ACGGGTTTGT GATGGTGCCA TCCGAGAGTT CTTGTTCCAG 
CAGCTAGGGA TGTTGGTGTC CTTTGTCAGA AGTCATATTC GACCCTACAT GGATGAAATT 
GTCACCCTGA TGAGAGACTT CTGGGTCATG AACAACTCCA TACAGAGCAC AATTATCCTA 
CTTATTGAGC AAATTGTGGT AGCTCTGGGA GGCGAATTCA AACTTTATCT GCCTCAGCTC 
ATTCCTCACA TGTTACGGGT CTTCATGCAT GACAACAGCC AGGGTCGCAT CGTCTCTGTC 
AAGCTCTTGA ATGCGATCCA GCTCTTTGGA GCTAACTTGG ATGACTACCT TCATTTGTTA 
CTACCTCCCA TTGTAAAGCT CTTTGATGCC CCGGATGTCC CAGTGGTGGC TCGCAAAGCT 
GCTCTAGAAA CAGTGGACCG CTTGACGGAG TCCCTGGATT TCACTGATTA TGCTTCACGG 
ATTATTCATC CCATTGTGCG AACATTAGAT CAGAGCCCTG AGCTACGAAC CACTGCAATG 
GACACCCTCT CCTCTCTGGT GTTCCAGCTG GGAAAGAAGT ACCAGATTTT CATCCCGATG 
GTGAACAAAG TTCTGGTGCG ACACAGAATT AACCATCAAC GTTATGATGT TCTGATCTGC 
AGAATTGTAA AGGGCTACAC TCTTGCTGAT GAAGAAGAGG ATCCCCTGAT TTATCAGCAC 
CGAATGCTGA GAAGCAACCA GGGAGAAACC TTGGCCAGTG GTCCTGTGGA AACAGGCCCC 
ATGAAGAAAT TGCATGTTAG CACCATCAAC CTGCAGAAGG CCTGGGGAGC GGCAAGAAGA 
GTTTCCAAAG ATGACTGGTT GGAGTGGTTA AGAAGACTGA GTTTGGAGCT ACTGAAAGAT 
TCCTCCTCAC CATCCTTGCG CTCATGCTGG GCCCTGGCTC AGGCATATAA TCCCATGGCT 
CGAGATCTCT TCAATGCTGC TTTCGTTTCG TGCTGGTCTG AACTGAATGA AGACCAGCAG 
GATGAGCTGA TCCGAAGCAT TGAATTGGCC CTGACTTCTC AGGACATTGC AGAGGTCACT 
CAGACACTGC TGAACTTGGC AGAATTCATG GAGCACAGTG ACAAGGGTCC ACTGCCTTTG 
AGAGATGATA ATGGCATTGT CCTCCTTGGA GAAAGAGCTG CAAAATGTCG AGCGTACGCC 
AAGGCCCTTC ACTACAAAGA GCTGGAATTT CAGAAAGGTC CCACCCCGGC CATTCTAGAG 
TCTCTCATCA GCATTAACAA TAAACTGCAA CAGCCAGAAG CAGCTGCCGG GGTACTGGAA 
TATGCCATGA AGCACTTTGG GGAGCTGGAA ATTCAAGCTA CCTGGTATGA AAAGCTTCAT 
GAATGGGAAG ATGCTCTGGT GGCCTACGAC AAGAAGATGG ACACAAACAA AGATGATCCT 
GAGCTGATGC TAGGACGGAT GCGTTGCTTG GAAGCACTTG GTGAATGGGG GCAGCTCCAC 
CAGCAATGCT GTGAAAAGTG GACGCAGGTC AATGATGAAA CTCAAGCTAA GATGGCCAGG 
ATGGCTGCTG CTGCTGCCTG GGGTCTAGGC CAGTGGGACA GCATGGAAGA ATATACCTGC 
ATGATCCCAA GGGACACCCA CGATGGGGCT TTCTACAGAG CTGTACTGGC TCTTCATCAG 
GACCTTTTCT CACTGGCACA GCAGTGCATT GACAAAGCCA GAGATCTGCT AGACGCTGAG 
CTGACTGCCA TGGCAGGAGA GAGCTACAGT CGAGCCTATG GGGCGATGGT ATCCTGCCAG 
ATGCTGTCAG AGCTGGAAGA GGTTATCCAG TATAAACTTG TACCAGAGCG CCGTGAGATC 
ATCCGCCAGA TCTGGTGGGA ACGACTGCAG GGCTGTCAAC GAATCGTGGA AGACTGGCAA 
AGGATATTGA TGGTCCGATC TCTTGTTGTG AACCCTCATG AGGACATGAG GACTTGGCTC 
AAGTATGCCA GCTTGTGTGG CAAGAGCGGG AGACTGGCTT TGGCCCATAA GACACTTGTC 
CTGCTCTTGG GAGTAGATCC CTCTCGACAG CTGGATCACC CCCTGCCAAC TGTCCATCCC 
CAAGTGACTT ACGCGTACAT GAAGCACATG TGGAAGAGCG CCCGTAAGAT TGACGCGTTC 
CAGCACATGC AGCACTTTGT GCAGACGATG CAGCAGCAGG CGCAGCACGC CATTGCCACC 
GAGGACCAGC AGCACAAGCA GGAGCTCCAC AAGCTCATGG CACGGTGTTT CCTGAAACTG 
GGTGAGTGGC AGCTGAACCT GCAAGGCATC AACGAGAGCA CCATCCCCAA AGTCCTGCAG 
TACTACAGCG CTGCAACAGA GCACGATCGC AACTGGAGGA GAGCCTGGCA TGCCTGGGCA 
GTGATGAACT TTGAAGCAGT ACTTCACTAC AAGCATCAAA ACCAGGCCAG GGATGAGAAG 
AAGAAACTAC GTCACGCCAG CGGAGCCAGC ATCACCAGTG CTAACACCGA GGGCAGCAAC 
AGCGAGAGCG ATGCAGAGAG TGCAGAGAAC AGTCCCATCC CATCTCCAGT GCAGAAAAAA 
GTCACAGAGG ATTTATCCAA GACCCTCTTG ATGTACACAG TCCCTGCTGT CCAGGGCTTC 
TTCCGATCCA TCTCTCTATC TCGAGGAAAC AACCTACAAG ACACTCTAAG AGTCCTTACT 
CTGTGGTTTG ACTATGGTCA CTGGCCTGAT GTGAATGAGG CTTTGGTAGA AGGAGTCAAG 
GCAATCCAAA TAGACACTTG GCTGCAGGTT ATCCCTCAGC TCATTGCAAG AATTGACACC 
CCTCGACCAT TAGTAGGACG TCTCATTCAT CAGCTCCTCA CAGATATTGG ACGGTACCAT 
CCCCAGGCTT TGATCTATCC TTTGACTGTG GCCTCTAAAT CTACAACCAC AGCTCGCCAC 
AACGCGGCAA ATAAGATCCT GAAAAACATG TGTGAGCACA GTAACACTCT GGTGCAGCAG 
GCAATGATGG TGAGTGAAGA GCTGATCCGT GTGGCCATCC TATGGCATGA GATGTGGCAC 
GAAGGGTTGG AAGAAGCATC TCGTCTGTAC TTTGGAGAAA GGAATGTGAA AGGGATGTTT 
GAGGTGCTGG AGCCACTTCA TGCGATGATG GAGCGTGGGC CTCAGACTTT AAAAGAGACA 
TCCTTCAATC AGGCTTATGG CAGAGATCTA ATGGAAGCTC AGGAATGGTG CAGGAAGTAC 
ATGAAATCAG GAAACGTCAA AGACCTAACT CAGGCCTGGG ACCTGTATTA CCACGTGTTC 
CGCCGCATCT CAAAGCAGCT GCCTCAGCTC ACTTCTTTGG AACTACAGTA TGTGTCACCA 
AAACTCCTCA TGTGCCGGGA CCTGGAGCTG GCAGTGCCAG GAACATACGA TCCCAACCAG 
CCCATCATAC GCATTCAGTC CATCGCGCCC TCCCTGCAGG TCATCACCTC CAAGCAGCGG 
CCCAGGAAGT TAACCTTGAT GGGGAGCAAT GGGCATGAGT TTGTGTTCCT TCTGAAAGGT 
CATGAAGACC TTCGCCAAGA TGAAAGAGTG ATGCAGCTTT TTGGGTTGGT CAACACTCTG 
CTAGCAAATG ACCCAACTTC TCTGCGGAAA AACCTCAGCA TCCAGAGATA CGCTGTTATT 
CCACTGTCCA CAAACTCAGG GTTGATCGGT TGGGTGCCCC ACTGTGACAC GCTGCACGCG 
CTCATCCGGG ACTACCGAGA GAAGAAAAAG ATCCTGCTCA ACATCGAGCA TCGCATCATG 
CTGAGGATGG CTCCAGATTA TGACCACCTG ACTCTGATGC AGAAAGTAGA AGTGTTTGAA 
CATGCTGTCA ATAACACGGC TGGTGATGAC CTCGCCAAGC TGCTGTGGTT GAAAAGTCCA 
AGCTCAGAGG TGTGGTTTGA CCGAAGAACA AACTACACTC GTTCACTGGC TGTGATGTCT 
ATGGTTGGGT ACATTTTGGG CCTTGGGGAC AGACACCCCT CCAATCTGAT GTTAGACAGA 
CTGAGTGGAA AGATCCTGCA TATCGACTTC GGGGACTGTT TTGAGGTTGC AATGACAAGA 
GAAAAGTTCC CTGAGAAGAT TCCATTCAGG TTAACAAGGA TGCTGACAAA CGCTATGGAG 
GTGACAGGCC TCGATGGCAA TTACAGAATC ACCTGTCACA CGGTGATGGA AGTCCTGCGC 
GAGCACAAGG ACAGTGTGAT GGCTGTGCTT GAAGCCTTTG TGTATGATCC ATTGTTGAAC 
TGGCGGCTGA TGGACACAAA TACAAAGGGC AATAAGCGCT CGCGAACAAG AACAGACTCC 
TACTCAGCCA GTCAGTCAGT AGAAATGTTG GACAGTGTGG AATTGGGTGA GACAGCCCAT 
AAGAAAACTG GGACCACAGT GCCTGAATCC ATCCATTCCT TCATAGGAGA TGGCCTAGTG 
AAGCCAGAAG CTCTCAATAA GAAGGCAATT CAGATCATCA ACAGGGTTCG AGATAAGCTC 
ACTGGTCGAG ACTTCTCTCA TGACGAAACT CTTGACGTAC CCACACAAGT GGAATTGCTC 
ATTAAACAAG CTACTTCTCA TGAGAACCTG TGCCAGTGCT ACATTGGATG GTGTCCTTTC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796937.1
CDS	82..7647
Translation	MSGTVSILQQFANGLKSRSEETRAKAAKDLQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVSALRACLILTTQREPKEMQKPQWYRHTYEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFSTKPRHITPFTSFQSLQPSQSNALAGLLGYSAHQGIMGFATSPVPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRTSKNSLIQMTVLNLLPRLAAFRPSAFTADQYLPDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFQAYLPKVLEIIKAALPPKDFAHKRQKSVQVDATVFTCISMLARAMGPSIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPSLTNIPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRFCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGVGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALIVKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSQHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVRSHIRPYMDEIVTLMRDFWVMNNSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSQGRIVSVKLLNAIQLFGANLDDYLHLLLPPIVKLFDAPDVPVVARKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRTTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSNQGETLASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTQVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCQMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQRILMVRSLVVNPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKHMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRNWRRAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGASITSANTEGSNSESDAESAENSPIPSPVQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSASQSVEMLDSVELGETAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_014941451.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mechanistic target of rapamycin (serine/threonine kinase) (MTOR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014941451.1

ORF Insert Sequence:

ATGTCGGGCA CCGTCTCCAT CCTCCAACAA TTTGCCAATG GCCTGAAGAG TCGCAGTGAG 
GAGACGAGGG CGAAAGCTGC CAAGGACTTG CAGCATTATG TCACCATGGA GCTCCGCGAG 
ATGAGTCAAG AAGAATCTAC CAGGTTTTAT GATCAGCTGA ACCATCACAT ATTTGAGCTG 
GTCTCTAGCT CTGATGCAAA TGAAAGGAAG GGTGGCATTC TGGCTATAGC AAGCCTTATT 
GGTGTTGAAG GAGGTAACGC CACCCGTATT GGCAGGTTTG CCAACTACTT GAGGAATCTC 
TTGCCATCCA ATGACCCTGT TGTAATGGAG ATGGCTTCCA AGGCTATTGG GCGGCTTGCG 
ATGGCTGGTG ACACCTTCAC AGCTGAGTAT GTGGAGTTTG AGGTGAAACG AGCTCTGGAA 
TGGCTGGGAG CTGACAGGAA TGAAGGTCGA AGACATGCTG CTGTTCTTGT CCTGCGTGAG 
CTGGCAATCA GCGTGCCTAC CTTCTTCTTC CAACAAGTGC AGCCATTCTT TGACAATATA 
TTTGTGGCTG TGTGGGATCC CAAACAGGCC ATCCGAGAAG GAGCTGTTTC TGCCTTAAGA 
GCCTGCCTTA TCCTCACCAC TCAACGGGAG CCAAAAGAGA TGCAGAAGCC CCAGTGGTAC 
AGACATACAT ATGAAGAGGC TGAGAAAGGC TTTGATGAGA CTTTGGCCAA AGAGAAAGGA 
ATGAACCGTG ATGACCGAAT CCATGGTGCC TTACTGATCC TCAATGAGCT AGTGAGAATA 
AGCAGTATGG AGGGAGAGCG CCTTAGAGAG GAGATGGAAG AGATCACTCA GCAGCAGCTT 
GTGCATGACA AGTACTGCAA GGACCTGATG GGCTTCAGTA CCAAACCTCG CCACATCACT 
CCCTTCACTA GCTTCCAGTC TCTTCAGCCC TCTCAGTCCA ATGCTTTGGC TGGTCTTCTG 
GGGTACAGTG CCCACCAAGG AATCATGGGT TTCGCAACCT CCCCTGTCCC AGCAAAGTCT 
ACATTGGTAG AAAGTCGATG CTGCAGGGAT CTAATGGAAG AGAAGTTTGA TCAAGTGTGC 
CAGTGGGTTC TCAAGTGCAG AACCAGCAAA AATTCTTTGA TCCAGATGAC GGTTCTCAAT 
CTGCTACCCC GGCTGGCGGC TTTCCGTCCT TCCGCGTTCA CAGCTGATCA GTATCTTCCA 
GACACCATGA ATCATGTTCT CAGCTGTGTG AAGAAGGAGA AGGAGCGAAC AGCAGCCTTC 
CAGGCCTTGG GCTTGCTTTC TGTGGCCGTG AGGTCTGAGT TTCAAGCCTA CCTGCCAAAA 
GTCCTTGAAA TCATAAAAGC AGCTCTGCCA CCAAAGGACT TTGCCCACAA GAGGCAGAAG 
TCCGTGCAGG TTGATGCCAC AGTCTTCACC TGCATTAGTA TGTTGGCACG AGCCATGGGA 
CCCAGCATCC AGCAGGACAT CAAAGAGCTT TTGGAGCCGA TGCTGGCTGT GGGGCTGAGC 
CCTGCTCTAA CAGCGGTGCT GTACGACTTG AGTCGCCAGA TCCCTCAGCT TAAGAAGGAT 
ATCCAGGATG GGCTGCTGAA AATGCTCTCC CTTGTTCTAA TGCACAAGCC CCTGCGACAT 
CCGGGCATGC CGAAGGGCCT TGCCCACCAG CTCGCCTCCC CGAGTCTCAC CAACATCCCA 
GAGGCCAGCG ACGTGGGCAG CATCACCCTT GCTCTTCGAA CGCTGGGCAG CTTTGAGTTT 
GAAGGCCACT CTCTGACTCA GTTTGTTCGG CACTGTGCAG ACCACTTTCT GAACAGCGAG 
CACAAGGAGA TCCGGATGGA AGCAGCCCGC ACCTGCTCTC GCTTGCTCAC ACCTTCCATC 
CACTTGATCA GTGGCCATGC CCACGTGGTC AGTCAGACAG CAGTGCAGGT CGTGGCTGAC 
GTTCTCAGCA AACTGCTTGT GGTTGGAATA ACCGACCCAG ACCCTGATAT ACGTTTCTGT 
GTGTTGGCTT CTCTGGATGA GCGATTTGAT GCTCACCTTG CCCAGGCGGA AAATTTGCAA 
GCTCTTTTTG TGGCCCTGAA TGATCAAGTA TTTGAAATCC GCGAGCTGGC CATCTGTACT 
GTGGGTCGCC TGAGCAGCAT GAACCCTGCT TTTGTCATGC CTTTCCTGCG CAAGATGCTA 
ATCCAGATTC TAACAGAGCT GGAACACAGT GGTGTTGGCC GGATTAAAGA GCAAAGTGCC 
AGGATGTTGG GTCACCTGGT CTCTAACGCT CCCCGCCTCA TTCGTCCGTA TATGGAGCCT 
ATTCTTAAGG CGCTAATTGT GAAACTGAAA GATCCTGATC CAGATCCAAA TCCAGGGGTG 
ATTAACAATG TCCTAGCTAC CATAGGGGAG CTTGCACAGG TCAGTGGGCT GGAGATGAGG 
AAGTGGGTGG ATGAACTCTT CATTATCATT ATGGACATGC TGCAGGACTC CTCCCTGCTG 
GCTAAAAGAC AAGTGGCTCT TTGGACACTG GGGCAGCTTG TGGCCAGCAC TGGTTATGTG 
GTGGAACCAT ACAGAAAGTA CCCAACTTTG CTGGAGGTGC TTCTGAATTT CCTGAAGACT 
GAGCAGAATC AGGGCACCCG TAGAGAGGCT ATTCGTGTGT TAGGGTTGCT GGGTGCTTTG 
GACCCTTACA AACACAAAGT GAACATTGGC ATGATTGATC AGTCACGAGA TGCTTCAGCT 
GTCAGCCTCT CAGAGTCCAA ATCCAGCCAG GATTCTTCGG ATTACAGTAC CAGTGAGATG 
CTGGTGAACA TGGGAAACCT CCCTTTGGAT GAGTTCTATC CAGCTGTCTC TATGGTGGCA 
CTGATGAGAA TTTTCCGAGA CCAGTCCTTG TCCCAACACC ACACTATGGT AGTTCAGGCC 
ATCACCTTTA TTTTCAAATC TCTTGGGCTG AAGTGTGTGC AGTTCCTGCC CCAGGTCATG 
CCAACGTTCC TTAATGTAAT ACGGGTTTGT GATGGTGCCA TCCGAGAGTT CTTGTTCCAG 
CAGCTAGGGA TGTTGGTGTC CTTTGTCAGA AGTCATATTC GACCCTACAT GGATGAAATT 
GTCACCCTGA TGAGAGACTT CTGGGTCATG AACAACTCCA TACAGAGCAC AATTATCCTA 
CTTATTGAGC AAATTGTGGT AGCTCTGGGA GGCGAATTCA AACTTTATCT GCCTCAGCTC 
ATTCCTCACA TGTTACGGGT CTTCATGCAT GACAACAGCC AGGGTCGCAT CGTCTCTGTC 
AAGCTCTTGA ATGCGATCCA GCTCTTTGGA GCTAACTTGG ATGACTACCT TCATTTGTTA 
CTACCTCCCA TTGTAAAGCT CTTTGATGCC CCGGATGTCC CAGTGGTGGC TCGCAAAGCT 
GCTCTAGAAA CAGTGGACCG CTTGACGGAG TCCCTGGATT TCACTGATTA TGCTTCACGG 
ATTATTCATC CCATTGTGCG AACATTAGAT CAGAGCCCTG AGCTACGAAC CACTGCAATG 
GACACCCTCT CCTCTCTGGT GTTCCAGCTG GGAAAGAAGT ACCAGATTTT CATCCCGATG 
GTGAACAAAG TTCTGGTGCG ACACAGAATT AACCATCAAC GTTATGATGT TCTGATCTGC 
AGAATTGTAA AGGGCTACAC TCTTGCTGAT GAAGAAGAGG ATCCCCTGAT TTATCAGCAC 
CGAATGCTGA GAAGCAACCA GGGAGAAACC TTGGCCAGTG GTCCTGTGGA AACAGGCCCC 
ATGAAGAAAT TGCATGTTAG CACCATCAAC CTGCAGAAGG CCTGGGGAGC GGCAAGAAGA 
GTTTCCAAAG ATGACTGGTT GGAGTGGTTA AGAAGACTGA GTTTGGAGCT ACTGAAAGAT 
TCCTCCTCAC CATCCTTGCG CTCATGCTGG GCCCTGGCTC AGGCATATAA TCCCATGGCT 
CGAGATCTCT TCAATGCTGC TTTCGTTTCG TGCTGGTCTG AACTGAATGA AGACCAGCAG 
GATGAGCTGA TCCGAAGCAT TGAATTGGCC CTGACTTCTC AGGACATTGC AGAGGTCACT 
CAGACACTGC TGAACTTGGC AGAATTCATG GAGCACAGTG ACAAGGGTCC ACTGCCTTTG 
AGAGATGATA ATGGCATTGT CCTCCTTGGA GAAAGAGCTG CAAAATGTCG AGCGTACGCC 
AAGGCCCTTC ACTACAAAGA GCTGGAATTT CAGAAAGGTC CCACCCCGGC CATTCTAGAG 
TCTCTCATCA GCATTAACAA TAAACTGCAA CAGCCAGAAG CAGCTGCCGG GGTACTGGAA 
TATGCCATGA AGCACTTTGG GGAGCTGGAA ATTCAAGCTA CCTGGTATGA AAAGCTTCAT 
GAATGGGAAG ATGCTCTGGT GGCCTACGAC AAGAAGATGG ACACAAACAA AGATGATCCT 
GAGCTGATGC TAGGACGGAT GCGTTGCTTG GAAGCACTTG GTGAATGGGG GCAGCTCCAC 
CAGCAATGCT GTGAAAAGTG GACGCAGGTC AATGATGAAA CTCAAGCTAA GATGGCCAGG 
ATGGCTGCTG CTGCTGCCTG GGGTCTAGGC CAGTGGGACA GCATGGAAGA ATATACCTGC 
ATGATCCCAA GGGACACCCA CGATGGGGCT TTCTACAGAG CTGTACTGGC TCTTCATCAG 
GACCTTTTCT CACTGGCACA GCAGTGCATT GACAAAGCCA GAGATCTGCT AGACGCTGAG 
CTGACTGCCA TGGCAGGAGA GAGCTACAGT CGAGCCTATG GGGCGATGGT ATCCTGCCAG 
ATGCTGTCAG AGCTGGAAGA GGTTATCCAG TATAAACTTG TACCAGAGCG CCGTGAGATC 
ATCCGCCAGA TCTGGTGGGA ACGACTGCAG GGCTGTCAAC GAATCGTGGA AGACTGGCAA 
AGGATATTGA TGGTCCGATC TCTTGTTGTG AACCCTCATG AGGACATGAG GACTTGGCTC 
AAGTATGCCA GCTTGTGTGG CAAGAGCGGG AGACTGGCTT TGGCCCATAA GACACTTGTC 
CTGCTCTTGG GAGTAGATCC CTCTCGACAG CTGGATCACC CCCTGCCAAC TGTCCATCCC 
CAAGTGACTT ACGCGTACAT GAAGCACATG TGGAAGAGCG CCCGTAAGAT TGACGCGTTC 
CAGCACATGC AGCACTTTGT GCAGACGATG CAGCAGCAGG CGCAGCACGC CATTGCCACC 
GAGGACCAGC AGCACAAGCA GGAGCTCCAC AAGCTCATGG CACGGTGTTT CCTGAAACTG 
GGTGAGTGGC AGCTGAACCT GCAAGGCATC AACGAGAGCA CCATCCCCAA AGTCCTGCAG 
TACTACAGCG CTGCAACAGA GCACGATCGC AACTGGAGGA GAGCCTGGCA TGCCTGGGCA 
GTGATGAACT TTGAAGCAGT ACTTCACTAC AAGCATCAAA ACCAGGCCAG GGATGAGAAG 
AAGAAACTAC GTCACGCCAG CGGAGCCAGC ATCACCAGTG CTAACACCGA GGGCAGCAAC 
AGCGAGAGCG ATGCAGAGAG TGCAGAGAAC AGTCCCATCC CATCTCCAGT GCAGAAAAAA 
GTCACAGAGG ATTTATCCAA GACCCTCTTG ATGTACACAG TCCCTGCTGT CCAGGGCTTC 
TTCCGATCCA TCTCTCTATC TCGAGGAAAC AACCTACAAG ACACTCTAAG AGTCCTTACT 
CTGTGGTTTG ACTATGGTCA CTGGCCTGAT GTGAATGAGG CTTTGGTAGA AGGAGTCAAG 
GCAATCCAAA TAGACACTTG GCTGCAGGTT ATCCCTCAGC TCATTGCAAG AATTGACACC 
CCTCGACCAT TAGTAGGACG TCTCATTCAT CAGCTCCTCA CAGATATTGG ACGGTACCAT 
CCCCAGGCTT TGATCTATCC TTTGACTGTG GCCTCTAAAT CTACAACCAC AGCTCGCCAC 
AACGCGGCAA ATAAGATCCT GAAAAACATG TGTGAGCACA GTAACACTCT GGTGCAGCAG 
GCAATGATGG TGAGTGAAGA GCTGATCCGT GTGGCCATCC TATGGCATGA GATGTGGCAC 
GAAGGGTTGG AAGAAGCATC TCGTCTGTAC TTTGGAGAAA GGAATGTGAA AGGGATGTTT 
GAGGTGCTGG AGCCACTTCA TGCGATGATG GAGCGTGGGC CTCAGACTTT AAAAGAGACA 
TCCTTCAATC AGGCTTATGG CAGAGATCTA ATGGAAGCTC AGGAATGGTG CAGGAAGTAC 
ATGAAATCAG GAAACGTCAA AGACCTAACT CAGGCCTGGG ACCTGTATTA CCACGTGTTC 
CGCCGCATCT CAAAGCAGCT GCCTCAGCTC ACTTCTTTGG AACTACAGTA TGTGTCACCA 
AAACTCCTCA TGTGCCGGGA CCTGGAGCTG GCAGTGCCAG GAACATACGA TCCCAACCAG 
CCCATCATAC GCATTCAGTC CATCGCGCCC TCCCTGCAGG TCATCACCTC CAAGCAGCGG 
CCCAGGAAGT TAACCTTGAT GGGGAGCAAT GGGCATGAGT TTGTGTTCCT TCTGAAAGGT 
CATGAAGACC TTCGCCAAGA TGAAAGAGTG ATGCAGCTTT TTGGGTTGGT CAACACTCTG 
CTAGCAAATG ACCCAACTTC TCTGCGGAAA AACCTCAGCA TCCAGAGATA CGCTGTTATT 
CCACTGTCCA CAAACTCAGG GTTGATCGGT TGGGTGCCCC ACTGTGACAC GCTGCACGCG 
CTCATCCGGG ACTACCGAGA GAAGAAAAAG ATCCTGCTCA ACATCGAGCA TCGCATCATG 
CTGAGGATGG CTCCAGATTA TGACCACCTG ACTCTGATGC AGAAAGTAGA AGTGTTTGAA 
CATGCTGTCA ATAACACGGC TGGTGATGAC CTCGCCAAGC TGCTGTGGTT GAAAAGTCCA 
AGCTCAGAGG TGTGGTTTGA CCGAAGAACA AACTACACTC GTTCACTGGC TGTGATGTCT 
ATGGTTGGGT ACATTTTGGG CCTTGGGGAC AGACACCCCT CCAATCTGAT GTTAGACAGA 
CTGAGTGGAA AGATCCTGCA TATCGACTTC GGGGACTGTT TTGAGGTTGC AATGACAAGA 
GAAAAGTTCC CTGAGAAGAT TCCATTCAGG TTAACAAGGA TGCTGACAAA CGCTATGGAG 
GTGACAGGCC TCGATGGCAA TTACAGAATC ACCTGTCACA CGGTGATGGA AGTCCTGCGC 
GAGCACAAGG ACAGTGTGAT GGCTGTGCTT GAAGCCTTTG TGTATGATCC ATTGTTGAAC 
TGGCGGCTGA TGGACACAAA TACAAAGGGC AATAAGCGCT CGCGAACAAG AACAGACTCC 
TACTCAGCCA GTCAGTCAGT AGAAATGTTG GACAGTGTGG AATTGGGTGA GACAGCCCAT 
AAGAAAACTG GGACCACAGT GCCTGAATCC ATCCATTCCT TCATAGGAGA TGGCCTAGTG 
AAGCCAGAAG CTCTCAATAA GAAGGCAATT CAGATCATCA ACAGGGTTCG AGATAAGCTC 
ACTGGTCGAG ACTTCTCTCA TGACGAAACT CTTGACGTAC CCACACAAGT GGAATTGCTC 
ATTAAACAAG CTACTTCTCA TGAGAACCTG TGCCAGTGCT ACATTGGATG GTGTCCTTTC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MTOR cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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