TTLL5 cDNA ORF clone, Calidris pugnax(ruff)

  • Gene

  • Clones

  • gRNAs

The following TTLL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTLL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa64901 XM_014941807.1
Latest version!
Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa64902 XM_014941808.1
Latest version!
Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa64903 XM_014941809.1
Latest version!
Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa64901
    Clone ID Related Accession (Same CDS sequence) XM_014941807.1
    Accession Version XM_014941807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product tubulin polyglutamylase TTLL5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090839.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCGGTTG GGATGGCTCG AGACCTGGAG GAAGCTGGCT CGTCCTCGGA GGACGAGGAG 
    GAAGATGTAG ATGGGCTGGA AGATCATCCA TGTATCAAGT GGACTGGTGG CGGCTGCAGG
    CGGATCCCCA TCATGGTGTT TCATGCTGAA GCCATTCTGA CCAACGACAG CTACCTCCGC
    CTAATTGGAG AGCGCTACCA TTTGTCATAT AAGATTGTGC GAACAGACAG CCGTCTGGTT
    CGAAGCATTC TGACTGCCCA TGGCTTCCAT GAGGTGCACC CTAATAGCAG CGATTATAAT
    CTCATGTGGA CAGGATCACA CTTGAAGCCC TACTTGCTGC GTTCCCTTAC AGATATTCAA
    AAAGTCAATC ACTTCCCTAG GTCATATGAA CTGACACGAA AGGACAGACT GTACAAGAAT
    GTCAGTCGCA TGCAGCTGGC CCATGGGTTC AAGACATTCC ATATCTTACC TCAGACCTTT
    ATCCTCCCGG CGGAGTACCG GGACTTCTGC AATACATATT CTAAGGACAG AGGCCCATGG
    ATAGTGAAGC CTGTGGCCTC TTCCAGGGGT CGAGGAGTCT ACCTGATAAA CAACCCAAAC
    CAGATTGTCC TGGAAGATGA CATCCTGGTT TCTCGTTACA TCAGCAACCC CCTGCTCATA
    GATGATTTCA AATTCGATGT GCGCCTCTAT GTTCTCGTTA CATCCTATGA TCCACTTGTC
    ATCTATCTCT ACGAGGAAGG ATTGGCCAGG TTTGCAACAG TGAGGTATGA CCAGGCATCC
    AAGAACATTA AAAACCAGTT CATGCATCTG ACCAACTACA GTGTCAACAA GAAGAGTGGA
    GATTATGTCA GCTCTGATGA TCCTGAAGTA GAGGATTATG GAAACAAGTG GAGCATGAGT
    GCAATGCTGA GGTACTTAAA ACAAGAGGGG AGAGACACTG CAGCACTGAT GGCCAACGTG
    GAAGACCTGA TTATCAAGAC GGTAGTTTCT GCTGAGCTTG CCATTGCCAC AGCTTGTAAA
    AATTTTCTTT CACATCGTGG CAGCTGCTTT GAACTGTATG GTTTTGATGT CCTTATTGAT
    GACACTCTGA AGCCCTGGCT GTTGGAAGTG AACCTGTCCC CATCACTTGC CTGCGATGCT
    CCTTTGGACC TGAAGATCAA AGCCAGCATG CTCTCAGATA TGTTCACTCT TGTAGGTTTT
    GTGTGCCAGG ATCCAGGCCA GCGGTCAAGC CGGTCTGTTT ATCATTCATC TGAATCTGCC
    AGGAGAAATC CATACCAGAA GCTGCAGCGT CCTGTGTCCG CGCAGTTGCA AACAGCAAAC
    ACCAGATTGC GTACCCGTCC TCTGTCTGCC AGTGATGCTG AAATGAAAAA CTCGGCGCCC
    TCAGGGAGGG AAAAAGCAAC AGGAAGGCAA GGCAGCTCTA TGCTAGGCCT CTCCATAGAG
    GAGATGAAAG TTCTTCGTCG AGTGAGAGAG GAGAATGAAC GGAGAGGAGG CTTCATTCGG
    ATATTCCCTA CACCGTTAAC ATGGGACCTT TATGGATCCT TTTTAGAGCA CAAGACATCA
    ATGAACTACA TGCTGGCTAC ACATCTGTTT CAGGACAGGG ACAAGATGAA AGGCGACTTC
    ATCACTGGGA AATCCCGAGC AGGTTTGAAT GGAAGGCTGG ATACGAGGTT GGAAGCTGTG
    AACTCTCATG CTCTTTTTTA TGAGAGAAAG CTTGTTTCAC TGGAGTTACG GAAGCGCCAG
    CGATGTCATG GCAAGGCTAG AGCAGCACGG ACAAGATCAT CAGGGACATC AGAGCCAAGA
    AAGCTGTCCC TCAAGTCAGA CACAGAAGGT GAGGAGGAAG AGGAGGTGGA TGCAGATGAA
    GATGAAGAAC TAGATAGAAC TGTTGGCTCT CTTTTGGACA CCCAGGTGAA AAGCAAGCCA
    AAGCGCTCTG ACTTGGTGAA AACTACTTGT AAGGAGAAGT TGCCAAAAAA ACTTAACAGG
    AAAACTGGAG ATGGAGGAGA GCCGTTTCTT CAAAGAACAG ACTCAGAATC TCAGTTTAAC
    TTGCTGCAGA TTCTTCAGAA GCATGGAAAC CTGAGCAAAG TGCAAGCTCG CAGGGCTTTC
    TCTGCCTATC TACAGCATGT TCAGTTGCGA CTGATGGAGG CTGGTGACCA GATCCACAAT
    CCTGCCTGGG CTGCCAAAGA GGATGAGCAA ATGGAGCTTG TGGTACGCTT TCTGAAGCGA
    GCTGCCAGCA ATCTTCAGCA GTCCCTGAGG ATGCTGCTCC CCAGCCGTCG TTTAGGACTG
    AATGACCGTC GTCGTGCCCT GGCTCACCAG CTGGGCGAGT TCATCATCTG TTATAACAAG
    GAAACAGAGC AGATGGTTCA GAAGCGATCA AAAAAGAAAC AGGAAGAGGA GGAGGAGGAG
    GGAGTAAATC CTGAAGGTTT TCAGAACTTT ATTACCAAAG CAAGTGAACG TGACCTGGAG
    GAAGTGCTGA CATTTTACAC ACACAAAAAC AAGTCAGCAA GTGTCTTCCT AGGGACAAAC
    TCTACAAGCA CAAAATCCAG CACCACCAGT AATCAGACAG AGAAACAGCC TCAAGGAGAG
    CGTCCTGAGG TGGTGAAAAA AATAAAAGGG GATCAGCTCA AAAGCTTAGT TGCGGATCTG
    CGTACAGAAG GAACATTAAA AGGCTATAAA TCCAAAGAAG TTAAATCAAC CTTTCCAGAA
    GGACCCACCT CCTCTTTAAA TGCTGAAGTG AAATTACCTC GGTGCAGCCC TCCTATTGCT
    TCTTCCTCTG TTTCTGGTGC CGCGTTCCAG AGAAGTACTG GTTCCTGCAT ACCATCTCAG
    CCTGCAGCCT CTGAAAATTC ACAGGTGCCT GGTCCCCATT CATTGCCAGC TCCTCCAGTT
    GGTCCCAGGC TGATTCAGTC CCCATCCCCA CTGCCCTCCT CACAGAGCAC AGCTCCTGAC
    AGCTCTTCTG TCTTCACAAA CCCAGTGTTC AGTCAGTCTT CTAGCCTTGC AGGGTTACAT
    CGTTATCATT CTGGTAGCTA CACTATTGGC CCATTCTCAT CTTTTTCAAG AGCTGCTCAG
    ATCTACAGCC AAAGATTGTC CCGACCATCC TCTGCAAAAG TAGGTTTGTG CCGTCGCAGT
    CCAGGTGGGC AGCGTGTGGG TTCAGCCAGG ATGCACAAGG ATGTAGAAGA TGCTTCTTCC
    CAGGACAAAC GTTACAGTCC CAGTATGGTA GCATCAGGAC TGCAGCAGCT AGCAGAAAAG
    CAAGCAGCCT GTCAATATTC CCCAGCAAGC CACATCAGTC TCCTTACACA ACAGTTAACA
    AACCTGAACT TGGCAAGTGG AGCTATAAGC AAGGGGAATG CAGCTGCCCC GCAGAGCTAC
    CGGTCACCGC TTGACAAAAG AGGGTCTTTG TGGGCTGTTG AGTCAGACGC TCCTATAGCT
    GACAAACGAT CTGTCTCTTC AGCAGTCAGG GCTCCAGAGA ACGATCATTT TGCCTGGGAA
    GGAGAGATGG AGGACAGTGT CTGCAGCAAG GTGGCAAAGA ACCCGCTGGT ACATCCCAAC
    TGTCAGCTCC ATTTTGCTGT GCAGCAACTT CGGCAGCAGA AACTTCAGTC ACGACAGCTG
    TTGGAGCAAA GCCAAGCCCG GCGCCAGGCT CTCTTTGCCA ACTACTCACA ATCCAGCACC
    AGCCATTTAT CTATGCCAGC TGGCTCTGAT GCCCGCAAGA CATCAAGTGC CACTTCTAGT
    ATCCAGAAGG CAGCCAGTTT GCAGAAAGTG ATGCCATCCC CGGGTACGCC GCCTCAGCTG
    GTTCCAAAGC CTCCAGCAAA CCACAGGCAA GCAGTGGTCA GAAAGGCTGC AGCCCAGAGG
    ATTTCCAAGC AACTGAAGGG ATTCCGGAAC AGCCTTCACA GTGCTGCATC CTGTGAGCCA
    GGGACAAATT CCACAGCCTC TGCTCACAGA GAAGGACAAA CAGCGAACCA CCAGGTGAGG
    AATCCAGGGT GTTGCTTCAA ACGTGGGGCA AAGGAAAAAG CAGCAGTGAC TTCATACCAC
    CACACTCCCA TAACAACCTG GAAAGAGCAA GGAGGAGGCC CCGGTGAGAG GACAACCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014797293.1
    CDS191..4273
    Translation

    Target ORF information:

    RefSeq Version XM_014941807.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014941807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCGGTTG GGATGGCTCG AGACCTGGAG GAAGCTGGCT CGTCCTCGGA GGACGAGGAG 
    GAAGATGTAG ATGGGCTGGA AGATCATCCA TGTATCAAGT GGACTGGTGG CGGCTGCAGG
    CGGATCCCCA TCATGGTGTT TCATGCTGAA GCCATTCTGA CCAACGACAG CTACCTCCGC
    CTAATTGGAG AGCGCTACCA TTTGTCATAT AAGATTGTGC GAACAGACAG CCGTCTGGTT
    CGAAGCATTC TGACTGCCCA TGGCTTCCAT GAGGTGCACC CTAATAGCAG CGATTATAAT
    CTCATGTGGA CAGGATCACA CTTGAAGCCC TACTTGCTGC GTTCCCTTAC AGATATTCAA
    AAAGTCAATC ACTTCCCTAG GTCATATGAA CTGACACGAA AGGACAGACT GTACAAGAAT
    GTCAGTCGCA TGCAGCTGGC CCATGGGTTC AAGACATTCC ATATCTTACC TCAGACCTTT
    ATCCTCCCGG CGGAGTACCG GGACTTCTGC AATACATATT CTAAGGACAG AGGCCCATGG
    ATAGTGAAGC CTGTGGCCTC TTCCAGGGGT CGAGGAGTCT ACCTGATAAA CAACCCAAAC
    CAGATTGTCC TGGAAGATGA CATCCTGGTT TCTCGTTACA TCAGCAACCC CCTGCTCATA
    GATGATTTCA AATTCGATGT GCGCCTCTAT GTTCTCGTTA CATCCTATGA TCCACTTGTC
    ATCTATCTCT ACGAGGAAGG ATTGGCCAGG TTTGCAACAG TGAGGTATGA CCAGGCATCC
    AAGAACATTA AAAACCAGTT CATGCATCTG ACCAACTACA GTGTCAACAA GAAGAGTGGA
    GATTATGTCA GCTCTGATGA TCCTGAAGTA GAGGATTATG GAAACAAGTG GAGCATGAGT
    GCAATGCTGA GGTACTTAAA ACAAGAGGGG AGAGACACTG CAGCACTGAT GGCCAACGTG
    GAAGACCTGA TTATCAAGAC GGTAGTTTCT GCTGAGCTTG CCATTGCCAC AGCTTGTAAA
    AATTTTCTTT CACATCGTGG CAGCTGCTTT GAACTGTATG GTTTTGATGT CCTTATTGAT
    GACACTCTGA AGCCCTGGCT GTTGGAAGTG AACCTGTCCC CATCACTTGC CTGCGATGCT
    CCTTTGGACC TGAAGATCAA AGCCAGCATG CTCTCAGATA TGTTCACTCT TGTAGGTTTT
    GTGTGCCAGG ATCCAGGCCA GCGGTCAAGC CGGTCTGTTT ATCATTCATC TGAATCTGCC
    AGGAGAAATC CATACCAGAA GCTGCAGCGT CCTGTGTCCG CGCAGTTGCA AACAGCAAAC
    ACCAGATTGC GTACCCGTCC TCTGTCTGCC AGTGATGCTG AAATGAAAAA CTCGGCGCCC
    TCAGGGAGGG AAAAAGCAAC AGGAAGGCAA GGCAGCTCTA TGCTAGGCCT CTCCATAGAG
    GAGATGAAAG TTCTTCGTCG AGTGAGAGAG GAGAATGAAC GGAGAGGAGG CTTCATTCGG
    ATATTCCCTA CACCGTTAAC ATGGGACCTT TATGGATCCT TTTTAGAGCA CAAGACATCA
    ATGAACTACA TGCTGGCTAC ACATCTGTTT CAGGACAGGG ACAAGATGAA AGGCGACTTC
    ATCACTGGGA AATCCCGAGC AGGTTTGAAT GGAAGGCTGG ATACGAGGTT GGAAGCTGTG
    AACTCTCATG CTCTTTTTTA TGAGAGAAAG CTTGTTTCAC TGGAGTTACG GAAGCGCCAG
    CGATGTCATG GCAAGGCTAG AGCAGCACGG ACAAGATCAT CAGGGACATC AGAGCCAAGA
    AAGCTGTCCC TCAAGTCAGA CACAGAAGGT GAGGAGGAAG AGGAGGTGGA TGCAGATGAA
    GATGAAGAAC TAGATAGAAC TGTTGGCTCT CTTTTGGACA CCCAGGTGAA AAGCAAGCCA
    AAGCGCTCTG ACTTGGTGAA AACTACTTGT AAGGAGAAGT TGCCAAAAAA ACTTAACAGG
    AAAACTGGAG ATGGAGGAGA GCCGTTTCTT CAAAGAACAG ACTCAGAATC TCAGTTTAAC
    TTGCTGCAGA TTCTTCAGAA GCATGGAAAC CTGAGCAAAG TGCAAGCTCG CAGGGCTTTC
    TCTGCCTATC TACAGCATGT TCAGTTGCGA CTGATGGAGG CTGGTGACCA GATCCACAAT
    CCTGCCTGGG CTGCCAAAGA GGATGAGCAA ATGGAGCTTG TGGTACGCTT TCTGAAGCGA
    GCTGCCAGCA ATCTTCAGCA GTCCCTGAGG ATGCTGCTCC CCAGCCGTCG TTTAGGACTG
    AATGACCGTC GTCGTGCCCT GGCTCACCAG CTGGGCGAGT TCATCATCTG TTATAACAAG
    GAAACAGAGC AGATGGTTCA GAAGCGATCA AAAAAGAAAC AGGAAGAGGA GGAGGAGGAG
    GGAGTAAATC CTGAAGGTTT TCAGAACTTT ATTACCAAAG CAAGTGAACG TGACCTGGAG
    GAAGTGCTGA CATTTTACAC ACACAAAAAC AAGTCAGCAA GTGTCTTCCT AGGGACAAAC
    TCTACAAGCA CAAAATCCAG CACCACCAGT AATCAGACAG AGAAACAGCC TCAAGGAGAG
    CGTCCTGAGG TGGTGAAAAA AATAAAAGGG GATCAGCTCA AAAGCTTAGT TGCGGATCTG
    CGTACAGAAG GAACATTAAA AGGCTATAAA TCCAAAGAAG TTAAATCAAC CTTTCCAGAA
    GGACCCACCT CCTCTTTAAA TGCTGAAGTG AAATTACCTC GGTGCAGCCC TCCTATTGCT
    TCTTCCTCTG TTTCTGGTGC CGCGTTCCAG AGAAGTACTG GTTCCTGCAT ACCATCTCAG
    CCTGCAGCCT CTGAAAATTC ACAGGTGCCT GGTCCCCATT CATTGCCAGC TCCTCCAGTT
    GGTCCCAGGC TGATTCAGTC CCCATCCCCA CTGCCCTCCT CACAGAGCAC AGCTCCTGAC
    AGCTCTTCTG TCTTCACAAA CCCAGTGTTC AGTCAGTCTT CTAGCCTTGC AGGGTTACAT
    CGTTATCATT CTGGTAGCTA CACTATTGGC CCATTCTCAT CTTTTTCAAG AGCTGCTCAG
    ATCTACAGCC AAAGATTGTC CCGACCATCC TCTGCAAAAG TAGGTTTGTG CCGTCGCAGT
    CCAGGTGGGC AGCGTGTGGG TTCAGCCAGG ATGCACAAGG ATGTAGAAGA TGCTTCTTCC
    CAGGACAAAC GTTACAGTCC CAGTATGGTA GCATCAGGAC TGCAGCAGCT AGCAGAAAAG
    CAAGCAGCCT GTCAATATTC CCCAGCAAGC CACATCAGTC TCCTTACACA ACAGTTAACA
    AACCTGAACT TGGCAAGTGG AGCTATAAGC AAGGGGAATG CAGCTGCCCC GCAGAGCTAC
    CGGTCACCGC TTGACAAAAG AGGGTCTTTG TGGGCTGTTG AGTCAGACGC TCCTATAGCT
    GACAAACGAT CTGTCTCTTC AGCAGTCAGG GCTCCAGAGA ACGATCATTT TGCCTGGGAA
    GGAGAGATGG AGGACAGTGT CTGCAGCAAG GTGGCAAAGA ACCCGCTGGT ACATCCCAAC
    TGTCAGCTCC ATTTTGCTGT GCAGCAACTT CGGCAGCAGA AACTTCAGTC ACGACAGCTG
    TTGGAGCAAA GCCAAGCCCG GCGCCAGGCT CTCTTTGCCA ACTACTCACA ATCCAGCACC
    AGCCATTTAT CTATGCCAGC TGGCTCTGAT GCCCGCAAGA CATCAAGTGC CACTTCTAGT
    ATCCAGAAGG CAGCCAGTTT GCAGAAAGTG ATGCCATCCC CGGGTACGCC GCCTCAGCTG
    GTTCCAAAGC CTCCAGCAAA CCACAGGCAA GCAGTGGTCA GAAAGGCTGC AGCCCAGAGG
    ATTTCCAAGC AACTGAAGGG ATTCCGGAAC AGCCTTCACA GTGCTGCATC CTGTGAGCCA
    GGGACAAATT CCACAGCCTC TGCTCACAGA GAAGGACAAA CAGCGAACCA CCAGGTGAGG
    AATCCAGGGT GTTGCTTCAA ACGTGGGGCA AAGGAAAAAG CAGCAGTGAC TTCATACCAC
    CACACTCCCA TAACAACCTG GAAAGAGCAA GGAGGAGGCC CCGGTGAGAG GACAACCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa64902
    Clone ID Related Accession (Same CDS sequence) XM_014941808.1
    Accession Version XM_014941808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product tubulin polyglutamylase TTLL5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090839.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCGGTTG GGATGGCTCG AGACCTGGAG GAAGCTGGCT CGTCCTCGGA GGACGAGGAG 
    GAAGATGTAG ATGGGCTGGA AGATCATCCA TGTATCAAGT GGACTGGTGG CGGCTGCAGG
    CGGATCCCCA TCATGGTGTT TCATGCTGAA GCCATTCTGA CCAACGACAG CTACCTCCGC
    CTAATTGGAG AGCGCTACCA TTTGTCATAT AAGATTGTGC GAACAGACAG CCGTCTGGTT
    CGAAGCATTC TGACTGCCCA TGGCTTCCAT GAGGTGCACC CTAATAGCAG CGATTATAAT
    CTCATGTGGA CAGGATCACA CTTGAAGCCC TACTTGCTGC GTTCCCTTAC AGATATTCAA
    AAAGTCAATC ACTTCCCTAG GTCATATGAA CTGACACGAA AGGACAGACT GTACAAGAAT
    GTCAGTCGCA TGCAGCTGGC CCATGGGTTC AAGACATTCC ATATCTTACC TCAGACCTTT
    ATCCTCCCGG CGGAGTACCG GGACTTCTGC AATACATATT CTAAGGACAG AGGCCCATGG
    ATAGTGAAGC CTGTGGCCTC TTCCAGGGGT CGAGGAGTCT ACCTGATAAA CAACCCAAAC
    CAGATTGTCC TGGAAGATGA CATCCTGGTT TCTCGTTACA TCAGCAACCC CCTGCTCATA
    GATGATTTCA AATTCGATGT GCGCCTCTAT GTTCTCGTTA CATCCTATGA TCCACTTGTC
    ATCTATCTCT ACGAGGAAGG ATTGGCCAGG TTTGCAACAG TGAGGTATGA CCAGGCATCC
    AAGAACATTA AAAACCAGTT CATGCATCTG ACCAACTACA GTGTCAACAA GAAGAGTGGA
    GATTATGTCA GCTCTGATGA TCCTGAAGTA GAGGATTATG GAAACAAGTG GAGCATGAGT
    GCAATGCTGA GGTACTTAAA ACAAGAGGGG AGAGACACTG CAGCACTGAT GGCCAACGTG
    GAAGACCTGA TTATCAAGAC GGTAGTTTCT GCTGAGCTTG CCATTGCCAC AGCTTGTAAA
    AATTTTCTTT CACATCGTGG CAGCTGCTTT GAACTGTATG GTTTTGATGT CCTTATTGAT
    GACACTCTGA AGCCCTGGCT GTTGGAAGTG AACCTGTCCC CATCACTTGC CTGCGATGCT
    CCTTTGGACC TGAAGATCAA AGCCAGCATG CTCTCAGATA TGTTCACTCT TGTAGGTTTT
    GTGTGCCAGG ATCCAGGCCA GCGGTCAAGC CGGTCTGTTT ATCATTCATC TGAATCTGCC
    AGGAGAAATC CATACCAGAA GCTGCAGCGT CCTGTGTCCG CGCAGTTGCA AACAGCAAAC
    ACCAGATTGC GTACCCGTCC TCTGTCTGCC AGTGATGCTG AAATGAAAAA CTCGGCGCCC
    TCAGGGAGGG AAAAAGCAAC AGGAAGGCAA GGCAGCTCTA TGCTAGGCCT CTCCATAGAG
    GAGATGAAAG TTCTTCGTCG AGTGAGAGAG GAGAATGAAC GGAGAGGAGG CTTCATTCGG
    ATATTCCCTA CACCGTTAAC ATGGGACCTT TATGGATCCT TTTTAGAGCA CAAGACATCA
    ATGAACTACA TGCTGGCTAC ACATCTGTTT CAGGACAGGG ACAAGATGAA AGGCGACTTC
    ATCACTGGGA AATCCCGAGC AGGTTTGAAT GGAAGGCTGG ATACGAGGTT GGAAGCTGTG
    AACTCTCATG CTCTTTTTTA TGAGAGAAAG CTTGTTTCAC TGGAGTTACG GAAGCGCCAG
    CGATGTCATG GCAAGGCTAG AGCAGCACGG ACAAGATCAT CAGGGACATC AGAGCCAAGA
    AAGCTGTCCC TCAAGTCAGA CACAGAAGGT GAGGAGGAAG AGGAGGTGGA TGCAGATGAA
    GATGAAGAAC TAGATAGAAC TGTTGGCTCT CTTTTGGACA CCCAGGTGAA AAGCAAGCCA
    AAGCGCTCTG ACTTGGTGAA AACTACTTGT AAGGAGAAGT TGCCAAAAAA ACTTAACAGG
    AAAACTGGAG ATGGAGGAGA GCCGTTTCTT CAAAGAACAG ACTCAGAATC TCAGTTTAAC
    TTGCTGCAGA TTCTTCAGAA GCATGGAAAC CTGAGCAAAG TGCAAGCTCG CAGGGCTTTC
    TCTGCCTATC TACAGCATGT TCAGTTGCGA CTGATGGAGG CTGGTGACCA GATCCACAAT
    CCTGCCTGGG CTGCCAAAGA GGATGAGCAA ATGGAGCTTG TGGTACGCTT TCTGAAGCGA
    GCTGCCAGCA ATCTTCAGCA GTCCCTGAGG ATGCTGCTCC CCAGCCGTCG TTTAGGACTG
    AATGACCGTC GTCGTGCCCT GGCTCACCAG CTGGGCGAGT TCATCATCTG TTATAACAAG
    GAAACAGAGC AGATGGTTCA GAAGCGATCA AAAAAGAAAC AGGAAGAGGA GGAGGAGGAG
    GGAGTAAATC CTGAAGGTTT TCAGAACTTT ATTACCAAAG CAAGTGAACG TGACCTGGAG
    GAAGTGCTGA CATTTTACAC ACACAAAAAC AAGTCAGCAA GTGTCTTCCT AGGGACAAAC
    TCTACAAGCA CAAAATCCAG CACCACCAGT AATCAGACAG AGAAACAGCC TCAAGGAGAG
    CGTCCTGAGG TGGTGAAAAA AATAAAAGGG GATCAGCTCA AAAGCTTAGT TGCGGATCTG
    CGTACAGAAG GAACATTAAA AGGCTATAAA TCCAAAGAAG TTAAATCAAC CTTTCCAGAA
    GGACCCACCT CCTCTTTAAA TGCTGAAGTG AAATTACCTC GGTGCAGCCC TCCTATTGCT
    TCTTCCTCTG TTTCTGGTGC CGCGTTCCAG AGAAGTACTG GTTCCTGCAT ACCATCTCAG
    CCTGCAGCCT CTGAAAATTC ACAGGTGCCT GGTCCCCATT CATTGCCAGC TCCTCCAGTT
    GGTCCCAGGC TGATTCAGTC CCCATCCCCA CTGCCCTCCT CACAGAGCAC AGCTCCTGAC
    AGCTCTTCTG TCTTCACAAA CCCAGTGTTC AGTCAGTCTT CTAGCCTTGC AGGGTTACAT
    CGTTATCATT CTGGTAGCTA CACTATTGGC CCATTCTCAT CTTTTTCAAG AGCTGCTCAG
    ATCTACAGCC AAAGATTGTC CCGACCATCC TCTGCAAAAG TAGGTTTGTG CCGTCGCAGT
    CCAGGTGGGC AGCGTGTGGG TTCAGCCAGG ATGCACAAGG ATGTAGAAGA TGCTTCTTCC
    CAGGACAAAC GTTACAGTCC CAGTATGGTA GCATCAGGAC TGCAGCAGCT AGCAGAAAAG
    CAAGCAGCCT GTCAATATTC CCCAGCAAGC CACATCAGTC TCCTTACACA ACAGTTAACA
    AACCTGAACT TGGCAAGTGG AGCTATAAGC AAGGGGAATG CAGCTGCCCC GCAGAGCTAC
    CGGTCACCGC TTGACAAAAG AGGGTCTTTG TGGGCTGTTG AGTCAGACGC TCCTATAGCT
    GACAAACGAT CTGTCTCTTC AGCAGTCAGG GCTCCAGAGA ACGATCATTT TGCCTGGGAA
    GGAGAGATGG AGGACAGTGT CTGCAGCAAG GTGGCAAAGA ACCCGCTGGT ACATCCCAAC
    TGTCAGCTCC ATTTTGCTGT GCAGCAACTT CGGCAGCAGA AACTTCAGTC ACGACAGCTG
    TTGGAGCAAA GCCAAGCCCG GCGCCAGGCT CTCTTTGCCA ACTACTCACA ATCCAGCACC
    AGCCATTTAT CTATGCCAGC TGGCTCTGAT GCCCGCAAGA CATCAAGTGC CACTTCTAGT
    ATCCAGAAGG CAGCCAGTTT GCAGAAAGTG ATGCCATCCC CGGGTACGCC GCCTCAGCTG
    GTTCCAAAGC CTCCAGCAAA CCACAGGCAA GCAGTGGTCA GAAAGGCTGC AGCCCAGAGG
    ATTTCCAACC TCTGCTCACA GAGAAGGACA AACAGCGAAC CACCAGGTGA GGAATCCAGG
    GTGTTGCTTC AAACGTGGGG CAAAGGAAAA AGCAGCAGTG ACTTCATACC ACCACACTCC
    CATAACAACC TGGAAAGAGC AAGGAGGAGG CCCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014797294.1
    CDS191..4189
    Translation

    Target ORF information:

    RefSeq Version XM_014941808.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014941808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCGGTTG GGATGGCTCG AGACCTGGAG GAAGCTGGCT CGTCCTCGGA GGACGAGGAG 
    GAAGATGTAG ATGGGCTGGA AGATCATCCA TGTATCAAGT GGACTGGTGG CGGCTGCAGG
    CGGATCCCCA TCATGGTGTT TCATGCTGAA GCCATTCTGA CCAACGACAG CTACCTCCGC
    CTAATTGGAG AGCGCTACCA TTTGTCATAT AAGATTGTGC GAACAGACAG CCGTCTGGTT
    CGAAGCATTC TGACTGCCCA TGGCTTCCAT GAGGTGCACC CTAATAGCAG CGATTATAAT
    CTCATGTGGA CAGGATCACA CTTGAAGCCC TACTTGCTGC GTTCCCTTAC AGATATTCAA
    AAAGTCAATC ACTTCCCTAG GTCATATGAA CTGACACGAA AGGACAGACT GTACAAGAAT
    GTCAGTCGCA TGCAGCTGGC CCATGGGTTC AAGACATTCC ATATCTTACC TCAGACCTTT
    ATCCTCCCGG CGGAGTACCG GGACTTCTGC AATACATATT CTAAGGACAG AGGCCCATGG
    ATAGTGAAGC CTGTGGCCTC TTCCAGGGGT CGAGGAGTCT ACCTGATAAA CAACCCAAAC
    CAGATTGTCC TGGAAGATGA CATCCTGGTT TCTCGTTACA TCAGCAACCC CCTGCTCATA
    GATGATTTCA AATTCGATGT GCGCCTCTAT GTTCTCGTTA CATCCTATGA TCCACTTGTC
    ATCTATCTCT ACGAGGAAGG ATTGGCCAGG TTTGCAACAG TGAGGTATGA CCAGGCATCC
    AAGAACATTA AAAACCAGTT CATGCATCTG ACCAACTACA GTGTCAACAA GAAGAGTGGA
    GATTATGTCA GCTCTGATGA TCCTGAAGTA GAGGATTATG GAAACAAGTG GAGCATGAGT
    GCAATGCTGA GGTACTTAAA ACAAGAGGGG AGAGACACTG CAGCACTGAT GGCCAACGTG
    GAAGACCTGA TTATCAAGAC GGTAGTTTCT GCTGAGCTTG CCATTGCCAC AGCTTGTAAA
    AATTTTCTTT CACATCGTGG CAGCTGCTTT GAACTGTATG GTTTTGATGT CCTTATTGAT
    GACACTCTGA AGCCCTGGCT GTTGGAAGTG AACCTGTCCC CATCACTTGC CTGCGATGCT
    CCTTTGGACC TGAAGATCAA AGCCAGCATG CTCTCAGATA TGTTCACTCT TGTAGGTTTT
    GTGTGCCAGG ATCCAGGCCA GCGGTCAAGC CGGTCTGTTT ATCATTCATC TGAATCTGCC
    AGGAGAAATC CATACCAGAA GCTGCAGCGT CCTGTGTCCG CGCAGTTGCA AACAGCAAAC
    ACCAGATTGC GTACCCGTCC TCTGTCTGCC AGTGATGCTG AAATGAAAAA CTCGGCGCCC
    TCAGGGAGGG AAAAAGCAAC AGGAAGGCAA GGCAGCTCTA TGCTAGGCCT CTCCATAGAG
    GAGATGAAAG TTCTTCGTCG AGTGAGAGAG GAGAATGAAC GGAGAGGAGG CTTCATTCGG
    ATATTCCCTA CACCGTTAAC ATGGGACCTT TATGGATCCT TTTTAGAGCA CAAGACATCA
    ATGAACTACA TGCTGGCTAC ACATCTGTTT CAGGACAGGG ACAAGATGAA AGGCGACTTC
    ATCACTGGGA AATCCCGAGC AGGTTTGAAT GGAAGGCTGG ATACGAGGTT GGAAGCTGTG
    AACTCTCATG CTCTTTTTTA TGAGAGAAAG CTTGTTTCAC TGGAGTTACG GAAGCGCCAG
    CGATGTCATG GCAAGGCTAG AGCAGCACGG ACAAGATCAT CAGGGACATC AGAGCCAAGA
    AAGCTGTCCC TCAAGTCAGA CACAGAAGGT GAGGAGGAAG AGGAGGTGGA TGCAGATGAA
    GATGAAGAAC TAGATAGAAC TGTTGGCTCT CTTTTGGACA CCCAGGTGAA AAGCAAGCCA
    AAGCGCTCTG ACTTGGTGAA AACTACTTGT AAGGAGAAGT TGCCAAAAAA ACTTAACAGG
    AAAACTGGAG ATGGAGGAGA GCCGTTTCTT CAAAGAACAG ACTCAGAATC TCAGTTTAAC
    TTGCTGCAGA TTCTTCAGAA GCATGGAAAC CTGAGCAAAG TGCAAGCTCG CAGGGCTTTC
    TCTGCCTATC TACAGCATGT TCAGTTGCGA CTGATGGAGG CTGGTGACCA GATCCACAAT
    CCTGCCTGGG CTGCCAAAGA GGATGAGCAA ATGGAGCTTG TGGTACGCTT TCTGAAGCGA
    GCTGCCAGCA ATCTTCAGCA GTCCCTGAGG ATGCTGCTCC CCAGCCGTCG TTTAGGACTG
    AATGACCGTC GTCGTGCCCT GGCTCACCAG CTGGGCGAGT TCATCATCTG TTATAACAAG
    GAAACAGAGC AGATGGTTCA GAAGCGATCA AAAAAGAAAC AGGAAGAGGA GGAGGAGGAG
    GGAGTAAATC CTGAAGGTTT TCAGAACTTT ATTACCAAAG CAAGTGAACG TGACCTGGAG
    GAAGTGCTGA CATTTTACAC ACACAAAAAC AAGTCAGCAA GTGTCTTCCT AGGGACAAAC
    TCTACAAGCA CAAAATCCAG CACCACCAGT AATCAGACAG AGAAACAGCC TCAAGGAGAG
    CGTCCTGAGG TGGTGAAAAA AATAAAAGGG GATCAGCTCA AAAGCTTAGT TGCGGATCTG
    CGTACAGAAG GAACATTAAA AGGCTATAAA TCCAAAGAAG TTAAATCAAC CTTTCCAGAA
    GGACCCACCT CCTCTTTAAA TGCTGAAGTG AAATTACCTC GGTGCAGCCC TCCTATTGCT
    TCTTCCTCTG TTTCTGGTGC CGCGTTCCAG AGAAGTACTG GTTCCTGCAT ACCATCTCAG
    CCTGCAGCCT CTGAAAATTC ACAGGTGCCT GGTCCCCATT CATTGCCAGC TCCTCCAGTT
    GGTCCCAGGC TGATTCAGTC CCCATCCCCA CTGCCCTCCT CACAGAGCAC AGCTCCTGAC
    AGCTCTTCTG TCTTCACAAA CCCAGTGTTC AGTCAGTCTT CTAGCCTTGC AGGGTTACAT
    CGTTATCATT CTGGTAGCTA CACTATTGGC CCATTCTCAT CTTTTTCAAG AGCTGCTCAG
    ATCTACAGCC AAAGATTGTC CCGACCATCC TCTGCAAAAG TAGGTTTGTG CCGTCGCAGT
    CCAGGTGGGC AGCGTGTGGG TTCAGCCAGG ATGCACAAGG ATGTAGAAGA TGCTTCTTCC
    CAGGACAAAC GTTACAGTCC CAGTATGGTA GCATCAGGAC TGCAGCAGCT AGCAGAAAAG
    CAAGCAGCCT GTCAATATTC CCCAGCAAGC CACATCAGTC TCCTTACACA ACAGTTAACA
    AACCTGAACT TGGCAAGTGG AGCTATAAGC AAGGGGAATG CAGCTGCCCC GCAGAGCTAC
    CGGTCACCGC TTGACAAAAG AGGGTCTTTG TGGGCTGTTG AGTCAGACGC TCCTATAGCT
    GACAAACGAT CTGTCTCTTC AGCAGTCAGG GCTCCAGAGA ACGATCATTT TGCCTGGGAA
    GGAGAGATGG AGGACAGTGT CTGCAGCAAG GTGGCAAAGA ACCCGCTGGT ACATCCCAAC
    TGTCAGCTCC ATTTTGCTGT GCAGCAACTT CGGCAGCAGA AACTTCAGTC ACGACAGCTG
    TTGGAGCAAA GCCAAGCCCG GCGCCAGGCT CTCTTTGCCA ACTACTCACA ATCCAGCACC
    AGCCATTTAT CTATGCCAGC TGGCTCTGAT GCCCGCAAGA CATCAAGTGC CACTTCTAGT
    ATCCAGAAGG CAGCCAGTTT GCAGAAAGTG ATGCCATCCC CGGGTACGCC GCCTCAGCTG
    GTTCCAAAGC CTCCAGCAAA CCACAGGCAA GCAGTGGTCA GAAAGGCTGC AGCCCAGAGG
    ATTTCCAACC TCTGCTCACA GAGAAGGACA AACAGCGAAC CACCAGGTGA GGAATCCAGG
    GTGTTGCTTC AAACGTGGGG CAAAGGAAAA AGCAGCAGTG ACTTCATACC ACCACACTCC
    CATAACAACC TGGAAAGAGC AAGGAGGAGG CCCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa64903
    Clone ID Related Accession (Same CDS sequence) XM_014941809.1
    Accession Version XM_014941809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product tubulin polyglutamylase TTLL5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090839.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGACAG GATCACACTT GAAGCCCTAC TTGCTGCGTT CCCTTACAGA TATTCAAAAA 
    GTCAATCACT TCCCTAGGTC ATATGAACTG ACACGAAAGG ACAGACTGTA CAAGAATGTC
    AGTCGCATGC AGCTGGCCCA TGGGTTCAAG ACATTCCATA TCTTACCTCA GACCTTTATC
    CTCCCGGCGG AGTACCGGGA CTTCTGCAAT ACATATTCTA AGGACAGAGG CCCATGGATA
    GTGAAGCCTG TGGCCTCTTC CAGGGGTCGA GGAGTCTACC TGATAAACAA CCCAAACCAG
    ATTGTCCTGG AAGATGACAT CCTGGTTTCT CGTTACATCA GCAACCCCCT GCTCATAGAT
    GATTTCAAAT TCGATGTGCG CCTCTATGTT CTCGTTACAT CCTATGATCC ACTTGTCATC
    TATCTCTACG AGGAAGGATT GGCCAGGTTT GCAACAGTGA GGTATGACCA GGCATCCAAG
    AACATTAAAA ACCAGTTCAT GCATCTGACC AACTACAGTG TCAACAAGAA GAGTGGAGAT
    TATGTCAGCT CTGATGATCC TGAAGTAGAG GATTATGGAA ACAAGTGGAG CATGAGTGCA
    ATGCTGAGGT ACTTAAAACA AGAGGGGAGA GACACTGCAG CACTGATGGC CAACGTGGAA
    GACCTGATTA TCAAGACGGT AGTTTCTGCT GAGCTTGCCA TTGCCACAGC TTGTAAAAAT
    TTTCTTTCAC ATCGTGGCAG CTGCTTTGAA CTGTATGGTT TTGATGTCCT TATTGATGAC
    ACTCTGAAGC CCTGGCTGTT GGAAGTGAAC CTGTCCCCAT CACTTGCCTG CGATGCTCCT
    TTGGACCTGA AGATCAAAGC CAGCATGCTC TCAGATATGT TCACTCTTGT AGGTTTTGTG
    TGCCAGGATC CAGGCCAGCG GTCAAGCCGG TCTGTTTATC ATTCATCTGA ATCTGCCAGG
    AGAAATCCAT ACCAGAAGCT GCAGCGTCCT GTGTCCGCGC AGTTGCAAAC AGCAAACACC
    AGATTGCGTA CCCGTCCTCT GTCTGCCAGT GATGCTGAAA TGAAAAACTC GGCGCCCTCA
    GGGAGGGAAA AAGCAACAGG AAGGCAAGGC AGCTCTATGC TAGGCCTCTC CATAGAGGAG
    ATGAAAGTTC TTCGTCGAGT GAGAGAGGAG AATGAACGGA GAGGAGGCTT CATTCGGATA
    TTCCCTACAC CGTTAACATG GGACCTTTAT GGATCCTTTT TAGAGCACAA GACATCAATG
    AACTACATGC TGGCTACACA TCTGTTTCAG GACAGGGACA AGATGAAAGG CGACTTCATC
    ACTGGGAAAT CCCGAGCAGG TTTGAATGGA AGGCTGGATA CGAGGTTGGA AGCTGTGAAC
    TCTCATGCTC TTTTTTATGA GAGAAAGCTT GTTTCACTGG AGTTACGGAA GCGCCAGCGA
    TGTCATGGCA AGGCTAGAGC AGCACGGACA AGATCATCAG GGACATCAGA GCCAAGAAAG
    CTGTCCCTCA AGTCAGACAC AGAAGGTGAG GAGGAAGAGG AGGTGGATGC AGATGAAGAT
    GAAGAACTAG ATAGAACTGT TGGCTCTCTT TTGGACACCC AGGTGAAAAG CAAGCCAAAG
    CGCTCTGACT TGGTGAAAAC TACTTGTAAG GAGAAGTTGC CAAAAAAACT TAACAGGAAA
    ACTGGAGATG GAGGAGAGCC GTTTCTTCAA AGAACAGACT CAGAATCTCA GTTTAACTTG
    CTGCAGATTC TTCAGAAGCA TGGAAACCTG AGCAAAGTGC AAGCTCGCAG GGCTTTCTCT
    GCCTATCTAC AGCATGTTCA GTTGCGACTG ATGGAGGCTG GTGACCAGAT CCACAATCCT
    GCCTGGGCTG CCAAAGAGGA TGAGCAAATG GAGCTTGTGG TACGCTTTCT GAAGCGAGCT
    GCCAGCAATC TTCAGCAGTC CCTGAGGATG CTGCTCCCCA GCCGTCGTTT AGGACTGAAT
    GACCGTCGTC GTGCCCTGGC TCACCAGCTG GGCGAGTTCA TCATCTGTTA TAACAAGGAA
    ACAGAGCAGA TGGTTCAGAA GCGATCAAAA AAGAAACAGG AAGAGGAGGA GGAGGAGGGA
    GTAAATCCTG AAGGTTTTCA GAACTTTATT ACCAAAGCAA GTGAACGTGA CCTGGAGGAA
    GTGCTGACAT TTTACACACA CAAAAACAAG TCAGCAAGTG TCTTCCTAGG GACAAACTCT
    ACAAGCACAA AATCCAGCAC CACCAGTAAT CAGACAGAGA AACAGCCTCA AGGAGAGCGT
    CCTGAGGTGG TGAAAAAAAT AAAAGGGGAT CAGCTCAAAA GCTTAGTTGC GGATCTGCGT
    ACAGAAGGAA CATTAAAAGG CTATAAATCC AAAGAAGTTA AATCAACCTT TCCAGAAGGA
    CCCACCTCCT CTTTAAATGC TGAAGTGAAA TTACCTCGGT GCAGCCCTCC TATTGCTTCT
    TCCTCTGTTT CTGGTGCCGC GTTCCAGAGA AGTACTGGTT CCTGCATACC ATCTCAGCCT
    GCAGCCTCTG AAAATTCACA GGTGCCTGGT CCCCATTCAT TGCCAGCTCC TCCAGTTGGT
    CCCAGGCTGA TTCAGTCCCC ATCCCCACTG CCCTCCTCAC AGAGCACAGC TCCTGACAGC
    TCTTCTGTCT TCACAAACCC AGTGTTCAGT CAGTCTTCTA GCCTTGCAGG GTTACATCGT
    TATCATTCTG GTAGCTACAC TATTGGCCCA TTCTCATCTT TTTCAAGAGC TGCTCAGATC
    TACAGCCAAA GATTGTCCCG ACCATCCTCT GCAAAAGTAG GTTTGTGCCG TCGCAGTCCA
    GGTGGGCAGC GTGTGGGTTC AGCCAGGATG CACAAGGATG TAGAAGATGC TTCTTCCCAG
    GACAAACGTT ACAGTCCCAG TATGGTAGCA TCAGGACTGC AGCAGCTAGC AGAAAAGCAA
    GCAGCCTGTC AATATTCCCC AGCAAGCCAC ATCAGTCTCC TTACACAACA GTTAACAAAC
    CTGAACTTGG CAAGTGGAGC TATAAGCAAG GGGAATGCAG CTGCCCCGCA GAGCTACCGG
    TCACCGCTTG ACAAAAGAGG GTCTTTGTGG GCTGTTGAGT CAGACGCTCC TATAGCTGAC
    AAACGATCTG TCTCTTCAGC AGTCAGGGCT CCAGAGAACG ATCATTTTGC CTGGGAAGGA
    GAGATGGAGG ACAGTGTCTG CAGCAAGGTG GCAAAGAACC CGCTGGTACA TCCCAACTGT
    CAGCTCCATT TTGCTGTGCA GCAACTTCGG CAGCAGAAAC TTCAGTCACG ACAGCTGTTG
    GAGCAAAGCC AAGCCCGGCG CCAGGCTCTC TTTGCCAACT ACTCACAATC CAGCACCAGC
    CATTTATCTA TGCCAGCTGG CTCTGATGCC CGCAAGACAT CAAGTGCCAC TTCTAGTATC
    CAGAAGGCAG CCAGTTTGCA GAAAGTGATG CCATCCCCGG GTACGCCGCC TCAGCTGGTT
    CCAAAGCCTC CAGCAAACCA CAGGCAAGCA GTGGTCAGAA AGGCTGCAGC CCAGAGGATT
    TCCAAGCAAC TGAAGGGATT CCGGAACAGC CTTCACAGTG CTGCATCCTG TGAGCCAGGG
    ACAAATTCCA CAGCCTCTGC TCACAGAGAA GGACAAACAG CGAACCACCA GGTGAGGAAT
    CCAGGGTGTT GCTTCAAACG TGGGGCAAAG GAAAAAGCAG CAGTGACTTC ATACCACCAC
    ACTCCCATAA CAACCTGGAA AGAGCAAGGA GGAGGCCCCG GTGAGAGGAC AACCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014797295.1
    CDS210..3989
    Translation

    Target ORF information:

    RefSeq Version XM_014941809.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tubulin tyrosine ligase like 5 (TTLL5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014941809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGACAG GATCACACTT GAAGCCCTAC TTGCTGCGTT CCCTTACAGA TATTCAAAAA 
    GTCAATCACT TCCCTAGGTC ATATGAACTG ACACGAAAGG ACAGACTGTA CAAGAATGTC
    AGTCGCATGC AGCTGGCCCA TGGGTTCAAG ACATTCCATA TCTTACCTCA GACCTTTATC
    CTCCCGGCGG AGTACCGGGA CTTCTGCAAT ACATATTCTA AGGACAGAGG CCCATGGATA
    GTGAAGCCTG TGGCCTCTTC CAGGGGTCGA GGAGTCTACC TGATAAACAA CCCAAACCAG
    ATTGTCCTGG AAGATGACAT CCTGGTTTCT CGTTACATCA GCAACCCCCT GCTCATAGAT
    GATTTCAAAT TCGATGTGCG CCTCTATGTT CTCGTTACAT CCTATGATCC ACTTGTCATC
    TATCTCTACG AGGAAGGATT GGCCAGGTTT GCAACAGTGA GGTATGACCA GGCATCCAAG
    AACATTAAAA ACCAGTTCAT GCATCTGACC AACTACAGTG TCAACAAGAA GAGTGGAGAT
    TATGTCAGCT CTGATGATCC TGAAGTAGAG GATTATGGAA ACAAGTGGAG CATGAGTGCA
    ATGCTGAGGT ACTTAAAACA AGAGGGGAGA GACACTGCAG CACTGATGGC CAACGTGGAA
    GACCTGATTA TCAAGACGGT AGTTTCTGCT GAGCTTGCCA TTGCCACAGC TTGTAAAAAT
    TTTCTTTCAC ATCGTGGCAG CTGCTTTGAA CTGTATGGTT TTGATGTCCT TATTGATGAC
    ACTCTGAAGC CCTGGCTGTT GGAAGTGAAC CTGTCCCCAT CACTTGCCTG CGATGCTCCT
    TTGGACCTGA AGATCAAAGC CAGCATGCTC TCAGATATGT TCACTCTTGT AGGTTTTGTG
    TGCCAGGATC CAGGCCAGCG GTCAAGCCGG TCTGTTTATC ATTCATCTGA ATCTGCCAGG
    AGAAATCCAT ACCAGAAGCT GCAGCGTCCT GTGTCCGCGC AGTTGCAAAC AGCAAACACC
    AGATTGCGTA CCCGTCCTCT GTCTGCCAGT GATGCTGAAA TGAAAAACTC GGCGCCCTCA
    GGGAGGGAAA AAGCAACAGG AAGGCAAGGC AGCTCTATGC TAGGCCTCTC CATAGAGGAG
    ATGAAAGTTC TTCGTCGAGT GAGAGAGGAG AATGAACGGA GAGGAGGCTT CATTCGGATA
    TTCCCTACAC CGTTAACATG GGACCTTTAT GGATCCTTTT TAGAGCACAA GACATCAATG
    AACTACATGC TGGCTACACA TCTGTTTCAG GACAGGGACA AGATGAAAGG CGACTTCATC
    ACTGGGAAAT CCCGAGCAGG TTTGAATGGA AGGCTGGATA CGAGGTTGGA AGCTGTGAAC
    TCTCATGCTC TTTTTTATGA GAGAAAGCTT GTTTCACTGG AGTTACGGAA GCGCCAGCGA
    TGTCATGGCA AGGCTAGAGC AGCACGGACA AGATCATCAG GGACATCAGA GCCAAGAAAG
    CTGTCCCTCA AGTCAGACAC AGAAGGTGAG GAGGAAGAGG AGGTGGATGC AGATGAAGAT
    GAAGAACTAG ATAGAACTGT TGGCTCTCTT TTGGACACCC AGGTGAAAAG CAAGCCAAAG
    CGCTCTGACT TGGTGAAAAC TACTTGTAAG GAGAAGTTGC CAAAAAAACT TAACAGGAAA
    ACTGGAGATG GAGGAGAGCC GTTTCTTCAA AGAACAGACT CAGAATCTCA GTTTAACTTG
    CTGCAGATTC TTCAGAAGCA TGGAAACCTG AGCAAAGTGC AAGCTCGCAG GGCTTTCTCT
    GCCTATCTAC AGCATGTTCA GTTGCGACTG ATGGAGGCTG GTGACCAGAT CCACAATCCT
    GCCTGGGCTG CCAAAGAGGA TGAGCAAATG GAGCTTGTGG TACGCTTTCT GAAGCGAGCT
    GCCAGCAATC TTCAGCAGTC CCTGAGGATG CTGCTCCCCA GCCGTCGTTT AGGACTGAAT
    GACCGTCGTC GTGCCCTGGC TCACCAGCTG GGCGAGTTCA TCATCTGTTA TAACAAGGAA
    ACAGAGCAGA TGGTTCAGAA GCGATCAAAA AAGAAACAGG AAGAGGAGGA GGAGGAGGGA
    GTAAATCCTG AAGGTTTTCA GAACTTTATT ACCAAAGCAA GTGAACGTGA CCTGGAGGAA
    GTGCTGACAT TTTACACACA CAAAAACAAG TCAGCAAGTG TCTTCCTAGG GACAAACTCT
    ACAAGCACAA AATCCAGCAC CACCAGTAAT CAGACAGAGA AACAGCCTCA AGGAGAGCGT
    CCTGAGGTGG TGAAAAAAAT AAAAGGGGAT CAGCTCAAAA GCTTAGTTGC GGATCTGCGT
    ACAGAAGGAA CATTAAAAGG CTATAAATCC AAAGAAGTTA AATCAACCTT TCCAGAAGGA
    CCCACCTCCT CTTTAAATGC TGAAGTGAAA TTACCTCGGT GCAGCCCTCC TATTGCTTCT
    TCCTCTGTTT CTGGTGCCGC GTTCCAGAGA AGTACTGGTT CCTGCATACC ATCTCAGCCT
    GCAGCCTCTG AAAATTCACA GGTGCCTGGT CCCCATTCAT TGCCAGCTCC TCCAGTTGGT
    CCCAGGCTGA TTCAGTCCCC ATCCCCACTG CCCTCCTCAC AGAGCACAGC TCCTGACAGC
    TCTTCTGTCT TCACAAACCC AGTGTTCAGT CAGTCTTCTA GCCTTGCAGG GTTACATCGT
    TATCATTCTG GTAGCTACAC TATTGGCCCA TTCTCATCTT TTTCAAGAGC TGCTCAGATC
    TACAGCCAAA GATTGTCCCG ACCATCCTCT GCAAAAGTAG GTTTGTGCCG TCGCAGTCCA
    GGTGGGCAGC GTGTGGGTTC AGCCAGGATG CACAAGGATG TAGAAGATGC TTCTTCCCAG
    GACAAACGTT ACAGTCCCAG TATGGTAGCA TCAGGACTGC AGCAGCTAGC AGAAAAGCAA
    GCAGCCTGTC AATATTCCCC AGCAAGCCAC ATCAGTCTCC TTACACAACA GTTAACAAAC
    CTGAACTTGG CAAGTGGAGC TATAAGCAAG GGGAATGCAG CTGCCCCGCA GAGCTACCGG
    TCACCGCTTG ACAAAAGAGG GTCTTTGTGG GCTGTTGAGT CAGACGCTCC TATAGCTGAC
    AAACGATCTG TCTCTTCAGC AGTCAGGGCT CCAGAGAACG ATCATTTTGC CTGGGAAGGA
    GAGATGGAGG ACAGTGTCTG CAGCAAGGTG GCAAAGAACC CGCTGGTACA TCCCAACTGT
    CAGCTCCATT TTGCTGTGCA GCAACTTCGG CAGCAGAAAC TTCAGTCACG ACAGCTGTTG
    GAGCAAAGCC AAGCCCGGCG CCAGGCTCTC TTTGCCAACT ACTCACAATC CAGCACCAGC
    CATTTATCTA TGCCAGCTGG CTCTGATGCC CGCAAGACAT CAAGTGCCAC TTCTAGTATC
    CAGAAGGCAG CCAGTTTGCA GAAAGTGATG CCATCCCCGG GTACGCCGCC TCAGCTGGTT
    CCAAAGCCTC CAGCAAACCA CAGGCAAGCA GTGGTCAGAA AGGCTGCAGC CCAGAGGATT
    TCCAAGCAAC TGAAGGGATT CCGGAACAGC CTTCACAGTG CTGCATCCTG TGAGCCAGGG
    ACAAATTCCA CAGCCTCTGC TCACAGAGAA GGACAAACAG CGAACCACCA GGTGAGGAAT
    CCAGGGTGTT GCTTCAAACG TGGGGCAAAG GAAAAAGCAG CAGTGACTTC ATACCACCAC
    ACTCCCATAA CAACCTGGAA AGAGCAAGGA GGAGGCCCCG GTGAGAGGAC AACCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.