NEK7 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK7
Entrez Gene ID	106888927
Full Name	NIMA related kinase 7
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014943485.1	XP_014798971.1	serine/threonine-protein kinase Nek7

Pathways from BioSystems
Gene Ontology

The following NEK7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa66139	XM_014943485.1 Latest version!	Calidris pugnax NIMA-related kinase 7 (NEK7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK7 ( XM_014943485.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014798971.1 Calidris pugnax NIMA-related kinase 7 (NEK7), transcript variant X1, mRNA.

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CloneID	OCa66139
Clone ID Related Accession (Same CDS sequence)	XM_014943485.1
Accession Version	XM_014943485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase Nek7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090852.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC AGTCACAAGG CATGCAGGGG CCTACTGCTC CACCATTTCA GCCACAGAAA 
GCCTTGCGAC CCGACATGGG CTATAATACA CTTGCCAATT TCCGAATAGA GAAAAAGATT 
GGCCGTGGGC AGTTCAGTGA AGTTTACAGA GCAACGTGCC TGTTGGATGG GGTACCAGTG 
GCACTGAAGA AGGTTCAGAT ATTTGATTTA ATGGATGCCA AAGCCCGTGC TGACTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTGAAT CATCCAAATG TAATTAAATA TTATGCCTCA 
TTCATTGAAG ACAATGAACT GAACATAGTG TTGGAATTAG CTGATGCTGG AGATCTCTCT 
AGAATGATAA AGCATTTTAA GAAGCAGAAG CGGTTGATTC CTGAAAGAAC AGTTTGGAAG 
TACTTCGTTC AGCTTTGCAG TGCCTTGGAA CATATGCATT CTCGTCGTGT TATGCACAGA 
GATATAAAGC CAGCTAATGT GTTCATTACA GCTACAGGGG TAGTAAAGCT TGGAGACCTT 
GGACTTGGTC GATTTTTCAG CTCCAAAACC ACAGCAGCAC ATTCTTTAGT TGGTACACCT 
TATTACATGT CTCCAGAGAG AATACACGAA AATGGTTACA ACTTCAAATC TGACATCTGG 
TCTCTGGGCT GTCTGCTGTA TGAGATGGCT GCACTGCAGA GTCCCTTTTA TGGTGATAAA 
ATGAACTTGT ACTCCCTATG CAAGAAGATA GAACAGTGTG ACTACCCACC TCTCCCTTCA 
GATCACTACT CAGAGGAACT GCGACAATTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTGACCTA CGTCTACGAT GTGGCGAAAC GTATGCACGC ACACACTGCC 
AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014798971.1
CDS	148..1056
Translation	MDEQSQGMQGPTAPPFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRATCLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHAHTASS

Target ORF information:

RefSeq Version	XM_014943485.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax NIMA-related kinase 7 (NEK7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014943485.1

ORF Insert Sequence:

ATGGATGAAC AGTCACAAGG CATGCAGGGG CCTACTGCTC CACCATTTCA GCCACAGAAA 
GCCTTGCGAC CCGACATGGG CTATAATACA CTTGCCAATT TCCGAATAGA GAAAAAGATT 
GGCCGTGGGC AGTTCAGTGA AGTTTACAGA GCAACGTGCC TGTTGGATGG GGTACCAGTG 
GCACTGAAGA AGGTTCAGAT ATTTGATTTA ATGGATGCCA AAGCCCGTGC TGACTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTGAAT CATCCAAATG TAATTAAATA TTATGCCTCA 
TTCATTGAAG ACAATGAACT GAACATAGTG TTGGAATTAG CTGATGCTGG AGATCTCTCT 
AGAATGATAA AGCATTTTAA GAAGCAGAAG CGGTTGATTC CTGAAAGAAC AGTTTGGAAG 
TACTTCGTTC AGCTTTGCAG TGCCTTGGAA CATATGCATT CTCGTCGTGT TATGCACAGA 
GATATAAAGC CAGCTAATGT GTTCATTACA GCTACAGGGG TAGTAAAGCT TGGAGACCTT 
GGACTTGGTC GATTTTTCAG CTCCAAAACC ACAGCAGCAC ATTCTTTAGT TGGTACACCT 
TATTACATGT CTCCAGAGAG AATACACGAA AATGGTTACA ACTTCAAATC TGACATCTGG 
TCTCTGGGCT GTCTGCTGTA TGAGATGGCT GCACTGCAGA GTCCCTTTTA TGGTGATAAA 
ATGAACTTGT ACTCCCTATG CAAGAAGATA GAACAGTGTG ACTACCCACC TCTCCCTTCA 
GATCACTACT CAGAGGAACT GCGACAATTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTGACCTA CGTCTACGAT GTGGCGAAAC GTATGCACGC ACACACTGCC 
AGCAGCTGA