COL18A1 cDNA ORF clone, Calidris pugnax(ruff)

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa70347 XM_014949230.1
Latest version!
Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCa70348 XM_014949231.1
Latest version!
Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa70349 XM_014949232.1
Latest version!
Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCa70350 XM_014949234.1
Latest version!
Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa70347
    Clone ID Related Accession (Same CDS sequence) XM_014949230.1
    Accession Version XM_014949230.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(XVIII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCCCC TTCCTCTTCG CTCGGAGCTC CTGCTCCTCC TGGCTTGCTG CTTCTCCTCC 
    TCCCAACCGC AGCTCCTGGA CTGGATTTGG GGCAGCACGA AGCCCACGGG AAGCCCAGCG
    ACATCCAGCA TGGGAACCAC AGGGCTGGAG CCACTCACCT CGGCGGCAGC TGCCACCCAA
    AGCACGTCCC CAGGATCGTG GGGTGTCCGG GTGGTGGAGG ATGTCACCAC CCTGCAGCTC
    CAGGAGCCGG ATCCCAACAC AGCAGCACCT GCAGGTGAGG GGACCACGGC CAAAACCAGC
    AAGCAGCGGG ATGGGAATGC CACGGGGCTG GTGAAGAGCA CGGCGTGGCC AAGCATCGCT
    CCTCCCCGCG GTGTGTCACC TGCCCCTGGC CAGGATGCGG CCAGCAGCTC CACCGAGGAC
    CTGCAGGTCC TGGGGACAGG GACCACCAAG GACCCGCAGC TCCTGGGGAT GGGGGACACC
    GAGGATCTGC AGATCCTGGG GACAGGGACT ACCAAGGACC CGCACCTCCT GGGGACAGGG
    ACCACCAAGG ACCTGCAGTT CCTGGGGATG GAGGACACTG AGGATCTGCA TCTCCTGGGG
    ACAGGGACCA CCGAGGACCC ACAGTTCCTG GGCACAGGGA CCACCAAGGA CCCGCAGTTC
    CTGGGGACAG GGATCACCAA GGACCCACAG ATCCTGGGAA CAGGGACTAC CAAGGACCCG
    CAGTTCCTGG GGACAGGGAC CACCGAGGAC CTGCAGCTCC TGGGGACAGG GACCACCAAG
    GACCCACAGA TCCTGGGAAC AGGGACTACC AAGGACCCGC AGTTCCTGGG GACAGGGACC
    ACCAAGGACC CGCAGCTCCT GGGGATGGAG GACACTGAGG ATTTGCAGCT CCTGGGGACA
    GGGACCACCA AGGACCCACA GTTCCTGGGG ACAGGGACCA CCGAGGACCC GCAGTTCCTG
    GGGACAGGAA CCACCGAGGA CCCGCAGTTC CTGGGGATGG GGGACACGGA GGATCTGCAG
    CTCCAGGGGA CAGGGACCCC CCCAGCAACG AGGACACAGG TGTCCTTGCA GAGACCGTCA
    GGTCCTTGGC CAGAGAACCT GAGCACCGAG GTCAGCCTCT TAGAGCTGAT CGGAGACCCC
    CCGCCGGAGG AGATCCTCAA AATTTACGGC CCCGACAACA ATCCCGGCTA CGTCTTCGGC
    CCCAATGCCA ACACAGGCCA GGTGGCCCGG TACCACCTGC CGAGCCCTTT CTACCGGGAC
    TTCTCCATCC TGTTCCACAT CCAGCCCACC ACACCCAGGG CTGGTGTGCT CTTTGCCATC
    ACAGACTCCT CGCAGAGCAT CATCTATGTG GGCATCAAGC TCTCAGAGCT GCGGGCGGGC
    AAGCAGCAGA TCATCTTCTA CTACACAGAG CCGGGCTCAC CCAGCTCATA TGCAGCAGCC
    ACCTTCACCG TGCCCACCTT GCTCAACCAG TGGACGCGCT TCGCCATCAG CGTGGAGGAG
    GACGAAGTTG TTCTCTACCT GGACTGTGAG GAGCACGAGC GGGTCCGTTT CGAGCGCTCC
    CCGGATGAGA TGGAGCTGGA AGATGGCTCC GGGCTCTTTG TTGCTCAGGC TGGAGGGGCT
    GACCCAGATA AATATCAGGG GGTGATCGCC GATTTGAAGC TGCGAGGGGA CCCCCGGGCG
    GCCGAGCGCC AGTGCGAGGA AGAGGAGGAT GACACCGAGG AGGTCTCTGG CGATTTTGGC
    AGCGGGGCAG AAGGTGGACA CCAACCCTCA GGGAAGGACA GGGGTGTTCC AGGGCTGGTG
    GATGCTGTCC CTGTCACCTC TCCTCCTGTG GCAGCGGGTG GTGGGATGAG GAGCGGTGGA
    GATTCCCCAC AGCAAGCTGA GAGGACCAGA GCAGAGGAGA CACCGCAGGT CTCCACGGGA
    GGCACCGGCC CCAAAGGTGA AAAAGGGGAG AAGGGCGAGC GTGGCCTGAA AGGGGACTCG
    GGGACCGGCA GCGTCGTGGG GATGGGCAGT GTCAAGGGTG AAAAGGGGGA GAAAGGAGAG
    CTGGGTGTTA AGGGCAGCGC TGGATTTGGC TACCCGGGCT CCAAGGGCCA GAAAGGAGAG
    CCAGGTAACC CCGGCCCGCC GGGGACCCTC TCGCGGCATT CTGATGGCTC GGTGGTGGAG
    CAGGTCACCG GTCCCCCAGG GCCACCAGGG AAGGATGGAG CCCCTGGCAG GGATGGCGAG
    CCCGGTGATC CTGGCGAGGA TGGCAAACCT GGCGATATGG GGCCCCAGGG GTTCCCCGGG
    ATGCCGGGGG AGCCTGGTCT GAAGGGGGAG AAGGGTGACC CAGGCGTAGG GCCAAGGGGA
    CCCCCTGGAC CACCCGGTCC TCCAGGACCT CCAGGACCAT CATCCAAGCC TGACAAGCTG
    ACCTTCATCG ACATGGAGGG CTCTGGCTTT GGCAGTGACC TGGAGACCCT GCGGGGTCCG
    CGAGGGCCAC CTGGTCCCCC AGGGCCACCC GGTGTGCCCG GTTTGCCAGG TGAGCCGGGA
    CGGTTTGGGA TGAACCGCAC AGACCTGCAG GGACTGCCAG GGCTGCCGGG CAGGGACGGG
    ACCCCTGGGT CCTCAGGGCC AGCGGGTCCT CAGGGTCCTC CTGGAAGAGA TGGGGCACCT
    GGGCAGCCAG GGCCCAAAGG AGAGCAGGGT GATGTGGGTG ACCTTGGCCT CCCTGGCGCA
    CCAGGACCCA AGGGCAGCAA GGGAGAAACA GGACCAGCAG GAGCCCCAGG AGAAATGGGG
    TTAGCTGGCC TTCCTGGGCC CATGGGACCC CGAGGGCTGC CGGGGCCCCC AGGTCCCCCA
    GGACCACCAG GGCTGGGCTA CGAGGCTGGA TTTGGTGACA TGGAGGGCTC GGGGCTGCCA
    TTTGCTGCCA GCTCCCCTGG ACCGCCTGGC CCCGAAGGAC CACAGGGGGT GCCTGGACTG
    CAGGGGGTAA AGGGGGAGGT CGGCAGCCCC GGGCAGCCCG GCCTGCCAGG GCCGAAGGGA
    GATGCTGGTG TGCCTGGCGT GGATGGCCGC CCTGGCCTGG AGGGCTTCCC CGGACCACAG
    GGACCCAAAG GTGACAGAGG CAGTCCCGGA GAGAAGGGTG AGCGAGGTCA AGATGGTGTG
    GGGCTGCCTG GCCCCCCTGG TCCACCTGGT CCCCCCGGAC AGGTTGTCGC TGTCTCAAAT
    AAAGATAAGT CTTTGGTGGC TTTGCCTGGT CCGGAGGGCA GACAGGGCCG CGCCGGCTTC
    CCGGGCCCAG TGGGACCGAA AGGAGATCAG GGGCCGTCTG GTCCCCAGGG CCCCCCGGGG
    TTGAAGGGGG AGAAGGGGGA GCCCGGTGTC ATCATCAGCC CCGATGGGAC GGTGGTCACT
    GCACAGGTGA AAGGAGAAAA GGGGGAGCCA GGGCCGCAGG GACCGATGGG ACCTTCAGGT
    CCCCAGGGAC GAGGAGGGAT GAAGGGAGAG ATTGGCTTTC CAGGAAGACC TGGACGTCCT
    GGCATGAACG GGCTGAAGGG TGAGAAGGGT GACCCCGCGG ATGTCACTAG CCTGCTGGGC
    TTGAGGGGTC CCCCAGGACC CCCAGGGCCC CCAGGGCCCC CTGGGCCACC CGGCAGCATA
    GTCTACGACA GCAGCAATGC CTTCAGTGAC TCTGGCCATC CCGTGCTGCC AGCTTTCCCC
    GGTTTCCACC AGTTTCAAGG ACAAAAGGGA GAGAAAGGTG ACGCCGGAGC CCCAGGACCC
    CCAGGCCACT TTGCCTACGA CCCGAGTCGC TTTGGTGCCA ACCTGCAGGG TGACAAGGGG
    GATCCGGGTC CCAAGGGTGA GAAGGGCGAG CCAGGCAGCA CCCCACTCTA TGGTCCCAGT
    GTCTCGGGGC TGCCAGGGCC TCCAGGGCCC CAGGGCTACC CTGGGCTGCC GGGTCCCAAG
    GGGGACAGTA TCGTTGGGCC ACCAGGGCCT CCTGGACCTC AGGGCCCCCC CGGGATTGGG
    TATGAGGGGC GGCAAGGCCC CCCTGGCCCT CCTGGGCCCC CCGGTCAACC CTCCTTCCCA
    GGCCCCCACC GACAAGCTAT CAGCATCCCT GGGCCCCCCG GACCACCGGG GCCCCCTGGA
    CCACCAGGCA CCGGCGGGAC GTCCTTGGGG CTGCACGCCC TGCCGAGCTA CCAGGCCATG
    CTGAGTGCTG CCCAGGAGCT GCCCGAGGGA GGGCTCGTCT TCCTCACCGA CCGGCAAGAG
    CTCTACGTCC GCCTGCGCGG GGGCTTCCGC CGGGTGCTGC TGGAGGAGCA CACCCTGATC
    CCCAGCTCAG CTCTGGACAA CGAGGTGTAC GACAAGCCCC CCAGAGTTCA CTATGCTGGC
    CCCCAGCCCC CGCTCCAGCC CCGCGGACCC CTGCATCCCC TTCGCAACCA CAGCCCACCG
    CCCACCACCC GCCCCTGGCA CAGGGACGAG GTGGTGGCCA ACCAGCACCG CCTGCCAGAG
    CAACCCCTTC TCCACCACCA GCACGAGCTC CTCAACAGCT ACTACATCCA CCGCCGGCCG
    GACCCGGCCC CCGTGGCCGC CCACGTGCAC CAGGACTTCC AGCCTGCCCT TCACCTGGTG
    GCCCTGAACG CCCCGCTGAG TGGCAGCATG CGTGGCATCC GGGGTGCGGA CTTCCAGTGC
    TTCCAGCAAG CCCGGCAGGT TGGGCTGGCC GGCACCTTCC GTGCCTTCCT CTCCTCCCGC
    CTGCAGGACC TCTACAGCAT CGTGCGCCGG GCCGACCGCG CCGCTGTGCC CATCGTCAAC
    CTCCGGGACG AAGTGCTCTT CAGTAACTGG GAAGCCCTTT TCACGGGCAA CGGGGCCCCA
    CTGCGAGCTG GCACCCGCAT CCTCTCCTTC GACGGCCGGG ATGTCCTGCA GGATGCAGGA
    TGGCCACAGA AGAGCATTTG GCACGGCTCA GACGCCAAGG GCCGGCGCTT GCCTGAGAGC
    TACTGCGAGA CGTGGCGGAC GGAGGAGCAC GCTGTCACCG GCCAGGCTTC CTCCCTGGGC
    TCGGGCAAGC TGCTGGAGCA GGTGGCCAGC AGCTGCCAGC ACACCTTCAT CGTCCTCTGC
    ATTGAGAACA GCTTCATGAC CGCCGCCAAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804716.1
    CDS99..5174
    Translation

    Target ORF information:

    RefSeq Version XM_014949230.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949230.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCCCC TTCCTCTTCG CTCGGAGCTC CTGCTCCTCC TGGCTTGCTG CTTCTCCTCC 
    TCCCAACCGC AGCTCCTGGA CTGGATTTGG GGCAGCACGA AGCCCACGGG AAGCCCAGCG
    ACATCCAGCA TGGGAACCAC AGGGCTGGAG CCACTCACCT CGGCGGCAGC TGCCACCCAA
    AGCACGTCCC CAGGATCGTG GGGTGTCCGG GTGGTGGAGG ATGTCACCAC CCTGCAGCTC
    CAGGAGCCGG ATCCCAACAC AGCAGCACCT GCAGGTGAGG GGACCACGGC CAAAACCAGC
    AAGCAGCGGG ATGGGAATGC CACGGGGCTG GTGAAGAGCA CGGCGTGGCC AAGCATCGCT
    CCTCCCCGCG GTGTGTCACC TGCCCCTGGC CAGGATGCGG CCAGCAGCTC CACCGAGGAC
    CTGCAGGTCC TGGGGACAGG GACCACCAAG GACCCGCAGC TCCTGGGGAT GGGGGACACC
    GAGGATCTGC AGATCCTGGG GACAGGGACT ACCAAGGACC CGCACCTCCT GGGGACAGGG
    ACCACCAAGG ACCTGCAGTT CCTGGGGATG GAGGACACTG AGGATCTGCA TCTCCTGGGG
    ACAGGGACCA CCGAGGACCC ACAGTTCCTG GGCACAGGGA CCACCAAGGA CCCGCAGTTC
    CTGGGGACAG GGATCACCAA GGACCCACAG ATCCTGGGAA CAGGGACTAC CAAGGACCCG
    CAGTTCCTGG GGACAGGGAC CACCGAGGAC CTGCAGCTCC TGGGGACAGG GACCACCAAG
    GACCCACAGA TCCTGGGAAC AGGGACTACC AAGGACCCGC AGTTCCTGGG GACAGGGACC
    ACCAAGGACC CGCAGCTCCT GGGGATGGAG GACACTGAGG ATTTGCAGCT CCTGGGGACA
    GGGACCACCA AGGACCCACA GTTCCTGGGG ACAGGGACCA CCGAGGACCC GCAGTTCCTG
    GGGACAGGAA CCACCGAGGA CCCGCAGTTC CTGGGGATGG GGGACACGGA GGATCTGCAG
    CTCCAGGGGA CAGGGACCCC CCCAGCAACG AGGACACAGG TGTCCTTGCA GAGACCGTCA
    GGTCCTTGGC CAGAGAACCT GAGCACCGAG GTCAGCCTCT TAGAGCTGAT CGGAGACCCC
    CCGCCGGAGG AGATCCTCAA AATTTACGGC CCCGACAACA ATCCCGGCTA CGTCTTCGGC
    CCCAATGCCA ACACAGGCCA GGTGGCCCGG TACCACCTGC CGAGCCCTTT CTACCGGGAC
    TTCTCCATCC TGTTCCACAT CCAGCCCACC ACACCCAGGG CTGGTGTGCT CTTTGCCATC
    ACAGACTCCT CGCAGAGCAT CATCTATGTG GGCATCAAGC TCTCAGAGCT GCGGGCGGGC
    AAGCAGCAGA TCATCTTCTA CTACACAGAG CCGGGCTCAC CCAGCTCATA TGCAGCAGCC
    ACCTTCACCG TGCCCACCTT GCTCAACCAG TGGACGCGCT TCGCCATCAG CGTGGAGGAG
    GACGAAGTTG TTCTCTACCT GGACTGTGAG GAGCACGAGC GGGTCCGTTT CGAGCGCTCC
    CCGGATGAGA TGGAGCTGGA AGATGGCTCC GGGCTCTTTG TTGCTCAGGC TGGAGGGGCT
    GACCCAGATA AATATCAGGG GGTGATCGCC GATTTGAAGC TGCGAGGGGA CCCCCGGGCG
    GCCGAGCGCC AGTGCGAGGA AGAGGAGGAT GACACCGAGG AGGTCTCTGG CGATTTTGGC
    AGCGGGGCAG AAGGTGGACA CCAACCCTCA GGGAAGGACA GGGGTGTTCC AGGGCTGGTG
    GATGCTGTCC CTGTCACCTC TCCTCCTGTG GCAGCGGGTG GTGGGATGAG GAGCGGTGGA
    GATTCCCCAC AGCAAGCTGA GAGGACCAGA GCAGAGGAGA CACCGCAGGT CTCCACGGGA
    GGCACCGGCC CCAAAGGTGA AAAAGGGGAG AAGGGCGAGC GTGGCCTGAA AGGGGACTCG
    GGGACCGGCA GCGTCGTGGG GATGGGCAGT GTCAAGGGTG AAAAGGGGGA GAAAGGAGAG
    CTGGGTGTTA AGGGCAGCGC TGGATTTGGC TACCCGGGCT CCAAGGGCCA GAAAGGAGAG
    CCAGGTAACC CCGGCCCGCC GGGGACCCTC TCGCGGCATT CTGATGGCTC GGTGGTGGAG
    CAGGTCACCG GTCCCCCAGG GCCACCAGGG AAGGATGGAG CCCCTGGCAG GGATGGCGAG
    CCCGGTGATC CTGGCGAGGA TGGCAAACCT GGCGATATGG GGCCCCAGGG GTTCCCCGGG
    ATGCCGGGGG AGCCTGGTCT GAAGGGGGAG AAGGGTGACC CAGGCGTAGG GCCAAGGGGA
    CCCCCTGGAC CACCCGGTCC TCCAGGACCT CCAGGACCAT CATCCAAGCC TGACAAGCTG
    ACCTTCATCG ACATGGAGGG CTCTGGCTTT GGCAGTGACC TGGAGACCCT GCGGGGTCCG
    CGAGGGCCAC CTGGTCCCCC AGGGCCACCC GGTGTGCCCG GTTTGCCAGG TGAGCCGGGA
    CGGTTTGGGA TGAACCGCAC AGACCTGCAG GGACTGCCAG GGCTGCCGGG CAGGGACGGG
    ACCCCTGGGT CCTCAGGGCC AGCGGGTCCT CAGGGTCCTC CTGGAAGAGA TGGGGCACCT
    GGGCAGCCAG GGCCCAAAGG AGAGCAGGGT GATGTGGGTG ACCTTGGCCT CCCTGGCGCA
    CCAGGACCCA AGGGCAGCAA GGGAGAAACA GGACCAGCAG GAGCCCCAGG AGAAATGGGG
    TTAGCTGGCC TTCCTGGGCC CATGGGACCC CGAGGGCTGC CGGGGCCCCC AGGTCCCCCA
    GGACCACCAG GGCTGGGCTA CGAGGCTGGA TTTGGTGACA TGGAGGGCTC GGGGCTGCCA
    TTTGCTGCCA GCTCCCCTGG ACCGCCTGGC CCCGAAGGAC CACAGGGGGT GCCTGGACTG
    CAGGGGGTAA AGGGGGAGGT CGGCAGCCCC GGGCAGCCCG GCCTGCCAGG GCCGAAGGGA
    GATGCTGGTG TGCCTGGCGT GGATGGCCGC CCTGGCCTGG AGGGCTTCCC CGGACCACAG
    GGACCCAAAG GTGACAGAGG CAGTCCCGGA GAGAAGGGTG AGCGAGGTCA AGATGGTGTG
    GGGCTGCCTG GCCCCCCTGG TCCACCTGGT CCCCCCGGAC AGGTTGTCGC TGTCTCAAAT
    AAAGATAAGT CTTTGGTGGC TTTGCCTGGT CCGGAGGGCA GACAGGGCCG CGCCGGCTTC
    CCGGGCCCAG TGGGACCGAA AGGAGATCAG GGGCCGTCTG GTCCCCAGGG CCCCCCGGGG
    TTGAAGGGGG AGAAGGGGGA GCCCGGTGTC ATCATCAGCC CCGATGGGAC GGTGGTCACT
    GCACAGGTGA AAGGAGAAAA GGGGGAGCCA GGGCCGCAGG GACCGATGGG ACCTTCAGGT
    CCCCAGGGAC GAGGAGGGAT GAAGGGAGAG ATTGGCTTTC CAGGAAGACC TGGACGTCCT
    GGCATGAACG GGCTGAAGGG TGAGAAGGGT GACCCCGCGG ATGTCACTAG CCTGCTGGGC
    TTGAGGGGTC CCCCAGGACC CCCAGGGCCC CCAGGGCCCC CTGGGCCACC CGGCAGCATA
    GTCTACGACA GCAGCAATGC CTTCAGTGAC TCTGGCCATC CCGTGCTGCC AGCTTTCCCC
    GGTTTCCACC AGTTTCAAGG ACAAAAGGGA GAGAAAGGTG ACGCCGGAGC CCCAGGACCC
    CCAGGCCACT TTGCCTACGA CCCGAGTCGC TTTGGTGCCA ACCTGCAGGG TGACAAGGGG
    GATCCGGGTC CCAAGGGTGA GAAGGGCGAG CCAGGCAGCA CCCCACTCTA TGGTCCCAGT
    GTCTCGGGGC TGCCAGGGCC TCCAGGGCCC CAGGGCTACC CTGGGCTGCC GGGTCCCAAG
    GGGGACAGTA TCGTTGGGCC ACCAGGGCCT CCTGGACCTC AGGGCCCCCC CGGGATTGGG
    TATGAGGGGC GGCAAGGCCC CCCTGGCCCT CCTGGGCCCC CCGGTCAACC CTCCTTCCCA
    GGCCCCCACC GACAAGCTAT CAGCATCCCT GGGCCCCCCG GACCACCGGG GCCCCCTGGA
    CCACCAGGCA CCGGCGGGAC GTCCTTGGGG CTGCACGCCC TGCCGAGCTA CCAGGCCATG
    CTGAGTGCTG CCCAGGAGCT GCCCGAGGGA GGGCTCGTCT TCCTCACCGA CCGGCAAGAG
    CTCTACGTCC GCCTGCGCGG GGGCTTCCGC CGGGTGCTGC TGGAGGAGCA CACCCTGATC
    CCCAGCTCAG CTCTGGACAA CGAGGTGTAC GACAAGCCCC CCAGAGTTCA CTATGCTGGC
    CCCCAGCCCC CGCTCCAGCC CCGCGGACCC CTGCATCCCC TTCGCAACCA CAGCCCACCG
    CCCACCACCC GCCCCTGGCA CAGGGACGAG GTGGTGGCCA ACCAGCACCG CCTGCCAGAG
    CAACCCCTTC TCCACCACCA GCACGAGCTC CTCAACAGCT ACTACATCCA CCGCCGGCCG
    GACCCGGCCC CCGTGGCCGC CCACGTGCAC CAGGACTTCC AGCCTGCCCT TCACCTGGTG
    GCCCTGAACG CCCCGCTGAG TGGCAGCATG CGTGGCATCC GGGGTGCGGA CTTCCAGTGC
    TTCCAGCAAG CCCGGCAGGT TGGGCTGGCC GGCACCTTCC GTGCCTTCCT CTCCTCCCGC
    CTGCAGGACC TCTACAGCAT CGTGCGCCGG GCCGACCGCG CCGCTGTGCC CATCGTCAAC
    CTCCGGGACG AAGTGCTCTT CAGTAACTGG GAAGCCCTTT TCACGGGCAA CGGGGCCCCA
    CTGCGAGCTG GCACCCGCAT CCTCTCCTTC GACGGCCGGG ATGTCCTGCA GGATGCAGGA
    TGGCCACAGA AGAGCATTTG GCACGGCTCA GACGCCAAGG GCCGGCGCTT GCCTGAGAGC
    TACTGCGAGA CGTGGCGGAC GGAGGAGCAC GCTGTCACCG GCCAGGCTTC CTCCCTGGGC
    TCGGGCAAGC TGCTGGAGCA GGTGGCCAGC AGCTGCCAGC ACACCTTCAT CGTCCTCTGC
    ATTGAGAACA GCTTCATGAC CGCCGCCAAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70348
    Clone ID Related Accession (Same CDS sequence) XM_014949231.1
    Accession Version XM_014949231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(XVIII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAACC TGAGCACCGA GGTCAGCCTC TTAGAGCTGA TCGGAGACCC CCCGCCGGAG 
    GAGATCCTCA AAATTTACGG CCCCGACAAC AATCCCGGCT ACGTCTTCGG CCCCAATGCC
    AACACAGGCC AGGTGGCCCG GTACCACCTG CCGAGCCCTT TCTACCGGGA CTTCTCCATC
    CTGTTCCACA TCCAGCCCAC CACACCCAGG GCTGGTGTGC TCTTTGCCAT CACAGACTCC
    TCGCAGAGCA TCATCTATGT GGGCATCAAG CTCTCAGAGC TGCGGGCGGG CAAGCAGCAG
    ATCATCTTCT ACTACACAGA GCCGGGCTCA CCCAGCTCAT ATGCAGCAGC CACCTTCACC
    GTGCCCACCT TGCTCAACCA GTGGACGCGC TTCGCCATCA GCGTGGAGGA GGACGAAGTT
    GTTCTCTACC TGGACTGTGA GGAGCACGAG CGGGTCCGTT TCGAGCGCTC CCCGGATGAG
    ATGGAGCTGG AAGATGGCTC CGGGCTCTTT GTTGCTCAGG CTGGAGGGGC TGACCCAGAT
    AAATATCAGG GGGTGATCGC CGATTTGAAG CTGCGAGGGG ACCCCCGGGC GGCCGAGCGC
    CAGTGCGAGG AAGAGGAGGA TGACACCGAG GAGGTCTCTG GCGATTTTGG CAGCGGGGCA
    GAAGGTGGAC ACCAACCCTC AGGGAAGGAC AGGGGTGTTC CAGGGCTGGT GGATGCTGTC
    CCTGTCACCT CTCCTCCTGT GGCAGCGGGT GGTGGGATGA GGAGCGGTGG AGATTCCCCA
    CAGCAAGCTG AGAGGACCAG AGCAGAGGAG ACACCGCAGG TCTCCACGGG AGGCACCGGC
    CCCAAAGGTG AAAAAGGGGA GAAGGGCGAG CGTGGCCTGA AAGGGGACTC GGGGACCGGC
    AGCGTCGTGG GGATGGGCAG TGTCAAGGGT GAAAAGGGGG AGAAAGGAGA GCTGGGTGTT
    AAGGGCAGCG CTGGATTTGG CTACCCGGGC TCCAAGGGCC AGAAAGGAGA GCCAGGTAAC
    CCCGGCCCGC CGGGGACCCT CTCGCGGCAT TCTGATGGCT CGGTGGTGGA GCAGGTCACC
    GGTCCCCCAG GGCCACCAGG GAAGGATGGA GCCCCTGGCA GGGATGGCGA GCCCGGTGAT
    CCTGGCGAGG ATGGCAAACC TGGCGATATG GGGCCCCAGG GGTTCCCCGG GATGCCGGGG
    GAGCCTGGTC TGAAGGGGGA GAAGGGTGAC CCAGGCGTAG GGCCAAGGGG ACCCCCTGGA
    CCACCCGGTC CTCCAGGACC TCCAGGACCA TCATCCAAGC CTGACAAGCT GACCTTCATC
    GACATGGAGG GCTCTGGCTT TGGCAGTGAC CTGGAGACCC TGCGGGGTCC GCGAGGGCCA
    CCTGGTCCCC CAGGGCCACC CGGTGTGCCC GGTTTGCCAG GTGAGCCGGG ACGGTTTGGG
    ATGAACCGCA CAGACCTGCA GGGACTGCCA GGGCTGCCGG GCAGGGACGG GACCCCTGGG
    TCCTCAGGGC CAGCGGGTCC TCAGGGTCCT CCTGGAAGAG ATGGGGCACC TGGGCAGCCA
    GGGCCCAAAG GAGAGCAGGG TGATGTGGGT GACCTTGGCC TCCCTGGCGC ACCAGGACCC
    AAGGGCAGCA AGGGAGAAAC AGGACCAGCA GGAGCCCCAG GAGAAATGGG GTTAGCTGGC
    CTTCCTGGGC CCATGGGACC CCGAGGGCTG CCGGGGCCCC CAGGTCCCCC AGGACCACCA
    GGGCTGGGCT ACGAGGCTGG ATTTGGTGAC ATGGAGGGCT CGGGGCTGCC ATTTGCTGCC
    AGCTCCCCTG GACCGCCTGG CCCCGAAGGA CCACAGGGGG TGCCTGGACT GCAGGGGGTA
    AAGGGGGAGG TCGGCAGCCC CGGGCAGCCC GGCCTGCCAG GGCCGAAGGG AGATGCTGGT
    GTGCCTGGCG TGGATGGCCG CCCTGGCCTG GAGGGCTTCC CCGGACCACA GGGACCCAAA
    GGTGACAGAG GCAGTCCCGG AGAGAAGGGT GAGCGAGGTC AAGATGGTGT GGGGCTGCCT
    GGCCCCCCTG GTCCACCTGG TCCCCCCGGA CAGGTTGTCG CTGTCTCAAA TAAAGATAAG
    TCTTTGGTGG CTTTGCCTGG TCCGGAGGGC AGACAGGGCC GCGCCGGCTT CCCGGGCCCA
    GTGGGACCGA AAGGAGATCA GGGGCCGTCT GGTCCCCAGG GCCCCCCGGG GTTGAAGGGG
    GAGAAGGGGG AGCCCGGTGT CATCATCAGC CCCGATGGGA CGGTGGTCAC TGCACAGGTG
    AAAGGAGAAA AGGGGGAGCC AGGGCCGCAG GGACCGATGG GACCTTCAGG TCCCCAGGGA
    CGAGGAGGGA TGAAGGGAGA GATTGGCTTT CCAGGAAGAC CTGGACGTCC TGGCATGAAC
    GGGCTGAAGG GTGAGAAGGG TGACCCCGCG GATGTCACTA GCCTGCTGGG CTTGAGGGGT
    CCCCCAGGAC CCCCAGGGCC CCCAGGGCCC CCTGGGCCAC CCGGCAGCAT AGTCTACGAC
    AGCAGCAATG CCTTCAGTGA CTCTGGCCAT CCCGTGCTGC CAGCTTTCCC CGGTTTCCAC
    CAGTTTCAAG GACAAAAGGG AGAGAAAGGT GACGCCGGAG CCCCAGGACC CCCAGGCCAC
    TTTGCCTACG ACCCGAGTCG CTTTGGTGCC AACCTGCAGG GTGACAAGGG GGATCCGGGT
    CCCAAGGGTG AGAAGGGCGA GCCAGGCAGC ACCCCACTCT ATGGTCCCAG TGTCTCGGGG
    CTGCCAGGGC CTCCAGGGCC CCAGGGCTAC CCTGGGCTGC CGGGTCCCAA GGGGGACAGT
    ATCGTTGGGC CACCAGGGCC TCCTGGACCT CAGGGCCCCC CCGGGATTGG GTATGAGGGG
    CGGCAAGGCC CCCCTGGCCC TCCTGGGCCC CCCGGTCAAC CCTCCTTCCC AGGCCCCCAC
    CGACAAGCTA TCAGCATCCC TGGGCCCCCC GGACCACCGG GGCCCCCTGG ACCACCAGGC
    ACCGGCGGGA CGTCCTTGGG GCTGCACGCC CTGCCGAGCT ACCAGGCCAT GCTGAGTGCT
    GCCCAGGAGC TGCCCGAGGG AGGGCTCGTC TTCCTCACCG ACCGGCAAGA GCTCTACGTC
    CGCCTGCGCG GGGGCTTCCG CCGGGTGCTG CTGGAGGAGC ACACCCTGAT CCCCAGCTCA
    GCTCTGGACA ACGAGGTGTA CGACAAGCCC CCCAGAGTTC ACTATGCTGG CCCCCAGCCC
    CCGCTCCAGC CCCGCGGACC CCTGCATCCC CTTCGCAACC ACAGCCCACC GCCCACCACC
    CGCCCCTGGC ACAGGGACGA GGTGGTGGCC AACCAGCACC GCCTGCCAGA GCAACCCCTT
    CTCCACCACC AGCACGAGCT CCTCAACAGC TACTACATCC ACCGCCGGCC GGACCCGGCC
    CCCGTGGCCG CCCACGTGCA CCAGGACTTC CAGCCTGCCC TTCACCTGGT GGCCCTGAAC
    GCCCCGCTGA GTGGCAGCAT GCGTGGCATC CGGGGTGCGG ACTTCCAGTG CTTCCAGCAA
    GCCCGGCAGG TTGGGCTGGC CGGCACCTTC CGTGCCTTCC TCTCCTCCCG CCTGCAGGAC
    CTCTACAGCA TCGTGCGCCG GGCCGACCGC GCCGCTGTGC CCATCGTCAA CCTCCGGGAC
    GAAGTGCTCT TCAGTAACTG GGAAGCCCTT TTCACGGGCA ACGGGGCCCC ACTGCGAGCT
    GGCACCCGCA TCCTCTCCTT CGACGGCCGG GATGTCCTGC AGGATGCAGG ATGGCCACAG
    AAGAGCATTT GGCACGGCTC AGACGCCAAG GGCCGGCGCT TGCCTGAGAG CTACTGCGAG
    ACGTGGCGGA CGGAGGAGCA CGCTGTCACC GGCCAGGCTT CCTCCCTGGG CTCGGGCAAG
    CTGCTGGAGC AGGTGGCCAG CAGCTGCCAG CACACCTTCA TCGTCCTCTG CATTGAGAAC
    AGCTTCATGA CCGCCGCCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804717.1
    CDS16..4002
    Translation

    Target ORF information:

    RefSeq Version XM_014949231.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAACC TGAGCACCGA GGTCAGCCTC TTAGAGCTGA TCGGAGACCC CCCGCCGGAG 
    GAGATCCTCA AAATTTACGG CCCCGACAAC AATCCCGGCT ACGTCTTCGG CCCCAATGCC
    AACACAGGCC AGGTGGCCCG GTACCACCTG CCGAGCCCTT TCTACCGGGA CTTCTCCATC
    CTGTTCCACA TCCAGCCCAC CACACCCAGG GCTGGTGTGC TCTTTGCCAT CACAGACTCC
    TCGCAGAGCA TCATCTATGT GGGCATCAAG CTCTCAGAGC TGCGGGCGGG CAAGCAGCAG
    ATCATCTTCT ACTACACAGA GCCGGGCTCA CCCAGCTCAT ATGCAGCAGC CACCTTCACC
    GTGCCCACCT TGCTCAACCA GTGGACGCGC TTCGCCATCA GCGTGGAGGA GGACGAAGTT
    GTTCTCTACC TGGACTGTGA GGAGCACGAG CGGGTCCGTT TCGAGCGCTC CCCGGATGAG
    ATGGAGCTGG AAGATGGCTC CGGGCTCTTT GTTGCTCAGG CTGGAGGGGC TGACCCAGAT
    AAATATCAGG GGGTGATCGC CGATTTGAAG CTGCGAGGGG ACCCCCGGGC GGCCGAGCGC
    CAGTGCGAGG AAGAGGAGGA TGACACCGAG GAGGTCTCTG GCGATTTTGG CAGCGGGGCA
    GAAGGTGGAC ACCAACCCTC AGGGAAGGAC AGGGGTGTTC CAGGGCTGGT GGATGCTGTC
    CCTGTCACCT CTCCTCCTGT GGCAGCGGGT GGTGGGATGA GGAGCGGTGG AGATTCCCCA
    CAGCAAGCTG AGAGGACCAG AGCAGAGGAG ACACCGCAGG TCTCCACGGG AGGCACCGGC
    CCCAAAGGTG AAAAAGGGGA GAAGGGCGAG CGTGGCCTGA AAGGGGACTC GGGGACCGGC
    AGCGTCGTGG GGATGGGCAG TGTCAAGGGT GAAAAGGGGG AGAAAGGAGA GCTGGGTGTT
    AAGGGCAGCG CTGGATTTGG CTACCCGGGC TCCAAGGGCC AGAAAGGAGA GCCAGGTAAC
    CCCGGCCCGC CGGGGACCCT CTCGCGGCAT TCTGATGGCT CGGTGGTGGA GCAGGTCACC
    GGTCCCCCAG GGCCACCAGG GAAGGATGGA GCCCCTGGCA GGGATGGCGA GCCCGGTGAT
    CCTGGCGAGG ATGGCAAACC TGGCGATATG GGGCCCCAGG GGTTCCCCGG GATGCCGGGG
    GAGCCTGGTC TGAAGGGGGA GAAGGGTGAC CCAGGCGTAG GGCCAAGGGG ACCCCCTGGA
    CCACCCGGTC CTCCAGGACC TCCAGGACCA TCATCCAAGC CTGACAAGCT GACCTTCATC
    GACATGGAGG GCTCTGGCTT TGGCAGTGAC CTGGAGACCC TGCGGGGTCC GCGAGGGCCA
    CCTGGTCCCC CAGGGCCACC CGGTGTGCCC GGTTTGCCAG GTGAGCCGGG ACGGTTTGGG
    ATGAACCGCA CAGACCTGCA GGGACTGCCA GGGCTGCCGG GCAGGGACGG GACCCCTGGG
    TCCTCAGGGC CAGCGGGTCC TCAGGGTCCT CCTGGAAGAG ATGGGGCACC TGGGCAGCCA
    GGGCCCAAAG GAGAGCAGGG TGATGTGGGT GACCTTGGCC TCCCTGGCGC ACCAGGACCC
    AAGGGCAGCA AGGGAGAAAC AGGACCAGCA GGAGCCCCAG GAGAAATGGG GTTAGCTGGC
    CTTCCTGGGC CCATGGGACC CCGAGGGCTG CCGGGGCCCC CAGGTCCCCC AGGACCACCA
    GGGCTGGGCT ACGAGGCTGG ATTTGGTGAC ATGGAGGGCT CGGGGCTGCC ATTTGCTGCC
    AGCTCCCCTG GACCGCCTGG CCCCGAAGGA CCACAGGGGG TGCCTGGACT GCAGGGGGTA
    AAGGGGGAGG TCGGCAGCCC CGGGCAGCCC GGCCTGCCAG GGCCGAAGGG AGATGCTGGT
    GTGCCTGGCG TGGATGGCCG CCCTGGCCTG GAGGGCTTCC CCGGACCACA GGGACCCAAA
    GGTGACAGAG GCAGTCCCGG AGAGAAGGGT GAGCGAGGTC AAGATGGTGT GGGGCTGCCT
    GGCCCCCCTG GTCCACCTGG TCCCCCCGGA CAGGTTGTCG CTGTCTCAAA TAAAGATAAG
    TCTTTGGTGG CTTTGCCTGG TCCGGAGGGC AGACAGGGCC GCGCCGGCTT CCCGGGCCCA
    GTGGGACCGA AAGGAGATCA GGGGCCGTCT GGTCCCCAGG GCCCCCCGGG GTTGAAGGGG
    GAGAAGGGGG AGCCCGGTGT CATCATCAGC CCCGATGGGA CGGTGGTCAC TGCACAGGTG
    AAAGGAGAAA AGGGGGAGCC AGGGCCGCAG GGACCGATGG GACCTTCAGG TCCCCAGGGA
    CGAGGAGGGA TGAAGGGAGA GATTGGCTTT CCAGGAAGAC CTGGACGTCC TGGCATGAAC
    GGGCTGAAGG GTGAGAAGGG TGACCCCGCG GATGTCACTA GCCTGCTGGG CTTGAGGGGT
    CCCCCAGGAC CCCCAGGGCC CCCAGGGCCC CCTGGGCCAC CCGGCAGCAT AGTCTACGAC
    AGCAGCAATG CCTTCAGTGA CTCTGGCCAT CCCGTGCTGC CAGCTTTCCC CGGTTTCCAC
    CAGTTTCAAG GACAAAAGGG AGAGAAAGGT GACGCCGGAG CCCCAGGACC CCCAGGCCAC
    TTTGCCTACG ACCCGAGTCG CTTTGGTGCC AACCTGCAGG GTGACAAGGG GGATCCGGGT
    CCCAAGGGTG AGAAGGGCGA GCCAGGCAGC ACCCCACTCT ATGGTCCCAG TGTCTCGGGG
    CTGCCAGGGC CTCCAGGGCC CCAGGGCTAC CCTGGGCTGC CGGGTCCCAA GGGGGACAGT
    ATCGTTGGGC CACCAGGGCC TCCTGGACCT CAGGGCCCCC CCGGGATTGG GTATGAGGGG
    CGGCAAGGCC CCCCTGGCCC TCCTGGGCCC CCCGGTCAAC CCTCCTTCCC AGGCCCCCAC
    CGACAAGCTA TCAGCATCCC TGGGCCCCCC GGACCACCGG GGCCCCCTGG ACCACCAGGC
    ACCGGCGGGA CGTCCTTGGG GCTGCACGCC CTGCCGAGCT ACCAGGCCAT GCTGAGTGCT
    GCCCAGGAGC TGCCCGAGGG AGGGCTCGTC TTCCTCACCG ACCGGCAAGA GCTCTACGTC
    CGCCTGCGCG GGGGCTTCCG CCGGGTGCTG CTGGAGGAGC ACACCCTGAT CCCCAGCTCA
    GCTCTGGACA ACGAGGTGTA CGACAAGCCC CCCAGAGTTC ACTATGCTGG CCCCCAGCCC
    CCGCTCCAGC CCCGCGGACC CCTGCATCCC CTTCGCAACC ACAGCCCACC GCCCACCACC
    CGCCCCTGGC ACAGGGACGA GGTGGTGGCC AACCAGCACC GCCTGCCAGA GCAACCCCTT
    CTCCACCACC AGCACGAGCT CCTCAACAGC TACTACATCC ACCGCCGGCC GGACCCGGCC
    CCCGTGGCCG CCCACGTGCA CCAGGACTTC CAGCCTGCCC TTCACCTGGT GGCCCTGAAC
    GCCCCGCTGA GTGGCAGCAT GCGTGGCATC CGGGGTGCGG ACTTCCAGTG CTTCCAGCAA
    GCCCGGCAGG TTGGGCTGGC CGGCACCTTC CGTGCCTTCC TCTCCTCCCG CCTGCAGGAC
    CTCTACAGCA TCGTGCGCCG GGCCGACCGC GCCGCTGTGC CCATCGTCAA CCTCCGGGAC
    GAAGTGCTCT TCAGTAACTG GGAAGCCCTT TTCACGGGCA ACGGGGCCCC ACTGCGAGCT
    GGCACCCGCA TCCTCTCCTT CGACGGCCGG GATGTCCTGC AGGATGCAGG ATGGCCACAG
    AAGAGCATTT GGCACGGCTC AGACGCCAAG GGCCGGCGCT TGCCTGAGAG CTACTGCGAG
    ACGTGGCGGA CGGAGGAGCA CGCTGTCACC GGCCAGGCTT CCTCCCTGGG CTCGGGCAAG
    CTGCTGGAGC AGGTGGCCAG CAGCTGCCAG CACACCTTCA TCGTCCTCTG CATTGAGAAC
    AGCTTCATGA CCGCCGCCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70349
    Clone ID Related Accession (Same CDS sequence) XM_014949232.1
    Accession Version XM_014949232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(XVIII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCGCCGG CCGGCCGGTG CCTGCCCGTC CCGACTCCCC TGTCCTTCTG CAGCGTGCTG 
    GGCACCCAGC ACGTCAGGGT CCCCAATTAC CTCCGCCACG GCAGCGAGGG GGAAATTCGG
    GCGGCTTTGC GTGAGTGGGA GGGGCTGCTG GAATCGCGGT GCCATCGCTA CCTGGAGTGG
    TTTCTCTGCT TGCTCCTGCT GCCCGGGTGT AGCCCTTCGG TCCCGGTAAC CCCCCCGCCG
    TGCCAGGGCT TCTGCGAGGC CGTCAGAGAC CTGTGTTGGA TGCACTTGGC CGGCGGGCGC
    CTGCCTCTGC CTTGTGATGC TCTCCCTCGG GAGGACGGAG GGTATTCTTG TGTCTTTATT
    AATGCTTCTG CAGAGAACCT GAGCACCGAG GTCAGCCTCT TAGAGCTGAT CGGAGACCCC
    CCGCCGGAGG AGATCCTCAA AATTTACGGC CCCGACAACA ATCCCGGCTA CGTCTTCGGC
    CCCAATGCCA ACACAGGCCA GGTGGCCCGG TACCACCTGC CGAGCCCTTT CTACCGGGAC
    TTCTCCATCC TGTTCCACAT CCAGCCCACC ACACCCAGGG CTGGTGTGCT CTTTGCCATC
    ACAGACTCCT CGCAGAGCAT CATCTATGTG GGCATCAAGC TCTCAGAGCT GCGGGCGGGC
    AAGCAGCAGA TCATCTTCTA CTACACAGAG CCGGGCTCAC CCAGCTCATA TGCAGCAGCC
    ACCTTCACCG TGCCCACCTT GCTCAACCAG TGGACGCGCT TCGCCATCAG CGTGGAGGAG
    GACGAAGTTG TTCTCTACCT GGACTGTGAG GAGCACGAGC GGGTCCGTTT CGAGCGCTCC
    CCGGATGAGA TGGAGCTGGA AGATGGCTCC GGGCTCTTTG TTGCTCAGGC TGGAGGGGCT
    GACCCAGATA AATATCAGGG GGTGATCGCC GATTTGAAGC TGCGAGGGGA CCCCCGGGCG
    GCCGAGCGCC AGTGCGAGGA AGAGGAGGAT GACACCGAGG AGGTCTCTGG CGATTTTGGC
    AGCGGGGCAG AAGGTGGACA CCAACCCTCA GGGAAGGACA GGGGTGTTCC AGGGCTGGTG
    GATGCTGTCC CTGTCACCTC TCCTCCTGTG GCAGCGGGTG GTGGGATGAG GAGCGGTGGA
    GATTCCCCAC AGCAAGCTGA GAGGACCAGA GCAGAGGAGA CACCGCAGGT CTCCACGGGA
    GGCACCGGCC CCAAAGGTGA AAAAGGGGAG AAGGGCGAGC GTGGCCTGAA AGGGGACTCG
    GGGACCGGCA GCGTCGTGGG GATGGGCAGT GTCAAGGGTG AAAAGGGGGA GAAAGGAGAG
    CTGGGTGTTA AGGGCAGCGC TGGATTTGGC TACCCGGGCT CCAAGGGCCA GAAAGGAGAG
    CCAGGTAACC CCGGCCCGCC GGGGACCCTC TCGCGGCATT CTGATGGCTC GGTGGTGGAG
    CAGGTCACCG GTCCCCCAGG GCCACCAGGG AAGGATGGAG CCCCTGGCAG GGATGGCGAG
    CCCGGTGATC CTGGCGAGGA TGGCAAACCT GGCGATATGG GGCCCCAGGG GTTCCCCGGG
    ATGCCGGGGG AGCCTGGTCT GAAGGGGGAG AAGGGTGACC CAGGCGTAGG GCCAAGGGGA
    CCCCCTGGAC CACCCGGTCC TCCAGGACCT CCAGGACCAT CATCCAAGCC TGACAAGCTG
    ACCTTCATCG ACATGGAGGG CTCTGGCTTT GGCAGTGACC TGGAGACCCT GCGGGGTCCG
    CGAGGGCCAC CTGGTCCCCC AGGGCCACCC GGTGTGCCCG GTTTGCCAGG TGAGCCGGGA
    CGGTTTGGGA TGAACCGCAC AGACCTGCAG GGACTGCCAG GGCTGCCGGG CAGGGACGGG
    ACCCCTGGGT CCTCAGGGCC AGCGGGTCCT CAGGGTCCTC CTGGAAGAGA TGGGGCACCT
    GGGCAGCCAG GGCCCAAAGG AGAGCAGGGT GATGTGGGTG ACCTTGGCCT CCCTGGCGCA
    CCAGGACCCA AGGGCAGCAA GGGAGAAACA GGACCAGCAG GAGCCCCAGG AGAAATGGGG
    TTAGCTGGCC TTCCTGGGCC CATGGGACCC CGAGGGCTGC CGGGGCCCCC AGGTCCCCCA
    GGACCACCAG GGCTGGGCTA CGAGGCTGGA TTTGGTGACA TGGAGGGCTC GGGGCTGCCA
    TTTGCTGCCA GCTCCCCTGG ACCGCCTGGC CCCGAAGGAC CACAGGGGGT GCCTGGACTG
    CAGGGGGTAA AGGGGGAGGT CGGCAGCCCC GGGCAGCCCG GCCTGCCAGG GCCGAAGGGA
    GATGCTGGTG TGCCTGGCGT GGATGGCCGC CCTGGCCTGG AGGGCTTCCC CGGACCACAG
    GGACCCAAAG GTGACAGAGG CAGTCCCGGA GAGAAGGGTG AGCGAGGTCA AGATGGTGTG
    GGGCTGCCTG GCCCCCCTGG TCCACCTGGT CCCCCCGGAC AGGTTGTCGC TGTCTCAAAT
    AAAGATAAGT CTTTGGTGGC TTTGCCTGGT CCGGAGGGCA GACAGGGCCG CGCCGGCTTC
    CCGGGCCCAG TGGGACCGAA AGGAGATCAG GGGCCGTCTG GTCCCCAGGG CCCCCCGGGG
    TTGAAGGGGG AGAAGGGGGA GCCCGGTGTC ATCATCAGCC CCGATGGGAC GGTGGTCACT
    GCACAGGTGA AAGGAGAAAA GGGGGAGCCA GGGCCGCAGG GACCGATGGG ACCTTCAGGT
    CCCCAGGGAC GAGGAGGGAT GAAGGGAGAG ATTGGCTTTC CAGGAAGACC TGGACGTCCT
    GGCATGAACG GGCTGAAGGG TGAGAAGGGT GACCCCGCGG ATGTCACTAG CCTGCTGGGC
    TTGAGGGGTC CCCCAGGACC CCCAGGGCCC CCAGGGCCCC CTGGGCCACC CGGCAGCATA
    GTCTACGACA GCAGCAATGC CTTCAGTGAC TCTGGCCATC CCGTGCTGCC AGCTTTCCCC
    GGTTTCCACC AGTTTCAAGG ACAAAAGGGA GAGAAAGGTG ACGCCGGAGC CCCAGGACCC
    CCAGGCCACT TTGCCTACGA CCCGAGTCGC TTTGGTGCCA ACCTGCAGGG TGACAAGGGG
    GATCCGGGTC CCAAGGGTGA GAAGGGCGAG CCAGGCAGCA CCCCACTCTA TGGTCCCAGT
    GTCTCGGGGC TGCCAGGGCC TCCAGGGCCC CAGGGCTACC CTGGGCTGCC GGGTCCCAAG
    GGGGACAGTA TCGTTGGGCC ACCAGGGCCT CCTGGACCTC AGGGCCCCCC CGGGATTGGG
    TATGAGGGGC GGCAAGGCCC CCCTGGCCCT CCTGGGCCCC CCGGTCAACC CTCCTTCCCA
    GGCCCCCACC GACAAGCTAT CAGCATCCCT GGGCCCCCCG GACCACCGGG GCCCCCTGGA
    CCACCAGGCA CCGGCGGGAC GTCCTTGGGG CTGCACGCCC TGCCGAGCTA CCAGGCCATG
    CTGAGTGCTG CCCAGGAGCT GCCCGAGGGA GGGCTCGTCT TCCTCACCGA CCGGCAAGAG
    CTCTACGTCC GCCTGCGCGG GGGCTTCCGC CGGGTGCTGC TGGAGGAGCA CACCCTGATC
    CCCAGCTCAG CTCTGGACAA CGAGGTGTAC GACAAGCCCC CCAGAGTTCA CTATGCTGGC
    CCCCAGCCCC CGCTCCAGCC CCGCGGACCC CTGCATCCCC TTCGCAACCA CAGCCCACCG
    CCCACCACCC GCCCCTGGCA CAGGGACGAG GTGGTGGCCA ACCAGCACCG CCTGCCAGAG
    CAACCCCTTC TCCACCACCA GCACGAGCTC CTCAACAGCT ACTACATCCA CCGCCGGCCG
    GACCCGGCCC CCGTGGCCGC CCACGTGCAC CAGGACTTCC AGCCTGCCCT TCACCTGGTG
    GCCCTGAACG CCCCGCTGAG TGGCAGCATG CGTGGCATCC GGGGTGCGGA CTTCCAGTGC
    TTCCAGCAAG CCCGGCAGGT TGGGCTGGCC GGCACCTTCC GTGCCTTCCT CTCCTCCCGC
    CTGCAGGACC TCTACAGCAT CGTGCGCCGG GCCGACCGCG CCGCTGTGCC CATCGTCAAC
    CTCCGGGACG AAGTGCTCTT CAGTAACTGG GAAGCCCTTT TCACGGGCAA CGGGGCCCCA
    CTGCGAGCTG GCACCCGCAT CCTCTCCTTC GACGGCCGGG ATGTCCTGCA GGATGCAGGA
    TGGCCACAGA AGAGCATTTG GCACGGCTCA GACGCCAAGG GCCGGCGCTT GCCTGAGAGC
    TACTGCGAGA CGTGGCGGAC GGAGGAGCAC GCTGTCACCG GCCAGGCTTC CTCCCTGGGC
    TCGGGCAAGC TGCTGGAGCA GGTGGCCAGC AGCTGCCAGC ACACCTTCAT CGTCCTCTGC
    ATTGAGAACA GCTTCATGAC CGCCGCCAAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804718.1
    CDS234..4589
    Translation

    Target ORF information:

    RefSeq Version XM_014949232.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCGCCGG CCGGCCGGTG CCTGCCCGTC CCGACTCCCC TGTCCTTCTG CAGCGTGCTG 
    GGCACCCAGC ACGTCAGGGT CCCCAATTAC CTCCGCCACG GCAGCGAGGG GGAAATTCGG
    GCGGCTTTGC GTGAGTGGGA GGGGCTGCTG GAATCGCGGT GCCATCGCTA CCTGGAGTGG
    TTTCTCTGCT TGCTCCTGCT GCCCGGGTGT AGCCCTTCGG TCCCGGTAAC CCCCCCGCCG
    TGCCAGGGCT TCTGCGAGGC CGTCAGAGAC CTGTGTTGGA TGCACTTGGC CGGCGGGCGC
    CTGCCTCTGC CTTGTGATGC TCTCCCTCGG GAGGACGGAG GGTATTCTTG TGTCTTTATT
    AATGCTTCTG CAGAGAACCT GAGCACCGAG GTCAGCCTCT TAGAGCTGAT CGGAGACCCC
    CCGCCGGAGG AGATCCTCAA AATTTACGGC CCCGACAACA ATCCCGGCTA CGTCTTCGGC
    CCCAATGCCA ACACAGGCCA GGTGGCCCGG TACCACCTGC CGAGCCCTTT CTACCGGGAC
    TTCTCCATCC TGTTCCACAT CCAGCCCACC ACACCCAGGG CTGGTGTGCT CTTTGCCATC
    ACAGACTCCT CGCAGAGCAT CATCTATGTG GGCATCAAGC TCTCAGAGCT GCGGGCGGGC
    AAGCAGCAGA TCATCTTCTA CTACACAGAG CCGGGCTCAC CCAGCTCATA TGCAGCAGCC
    ACCTTCACCG TGCCCACCTT GCTCAACCAG TGGACGCGCT TCGCCATCAG CGTGGAGGAG
    GACGAAGTTG TTCTCTACCT GGACTGTGAG GAGCACGAGC GGGTCCGTTT CGAGCGCTCC
    CCGGATGAGA TGGAGCTGGA AGATGGCTCC GGGCTCTTTG TTGCTCAGGC TGGAGGGGCT
    GACCCAGATA AATATCAGGG GGTGATCGCC GATTTGAAGC TGCGAGGGGA CCCCCGGGCG
    GCCGAGCGCC AGTGCGAGGA AGAGGAGGAT GACACCGAGG AGGTCTCTGG CGATTTTGGC
    AGCGGGGCAG AAGGTGGACA CCAACCCTCA GGGAAGGACA GGGGTGTTCC AGGGCTGGTG
    GATGCTGTCC CTGTCACCTC TCCTCCTGTG GCAGCGGGTG GTGGGATGAG GAGCGGTGGA
    GATTCCCCAC AGCAAGCTGA GAGGACCAGA GCAGAGGAGA CACCGCAGGT CTCCACGGGA
    GGCACCGGCC CCAAAGGTGA AAAAGGGGAG AAGGGCGAGC GTGGCCTGAA AGGGGACTCG
    GGGACCGGCA GCGTCGTGGG GATGGGCAGT GTCAAGGGTG AAAAGGGGGA GAAAGGAGAG
    CTGGGTGTTA AGGGCAGCGC TGGATTTGGC TACCCGGGCT CCAAGGGCCA GAAAGGAGAG
    CCAGGTAACC CCGGCCCGCC GGGGACCCTC TCGCGGCATT CTGATGGCTC GGTGGTGGAG
    CAGGTCACCG GTCCCCCAGG GCCACCAGGG AAGGATGGAG CCCCTGGCAG GGATGGCGAG
    CCCGGTGATC CTGGCGAGGA TGGCAAACCT GGCGATATGG GGCCCCAGGG GTTCCCCGGG
    ATGCCGGGGG AGCCTGGTCT GAAGGGGGAG AAGGGTGACC CAGGCGTAGG GCCAAGGGGA
    CCCCCTGGAC CACCCGGTCC TCCAGGACCT CCAGGACCAT CATCCAAGCC TGACAAGCTG
    ACCTTCATCG ACATGGAGGG CTCTGGCTTT GGCAGTGACC TGGAGACCCT GCGGGGTCCG
    CGAGGGCCAC CTGGTCCCCC AGGGCCACCC GGTGTGCCCG GTTTGCCAGG TGAGCCGGGA
    CGGTTTGGGA TGAACCGCAC AGACCTGCAG GGACTGCCAG GGCTGCCGGG CAGGGACGGG
    ACCCCTGGGT CCTCAGGGCC AGCGGGTCCT CAGGGTCCTC CTGGAAGAGA TGGGGCACCT
    GGGCAGCCAG GGCCCAAAGG AGAGCAGGGT GATGTGGGTG ACCTTGGCCT CCCTGGCGCA
    CCAGGACCCA AGGGCAGCAA GGGAGAAACA GGACCAGCAG GAGCCCCAGG AGAAATGGGG
    TTAGCTGGCC TTCCTGGGCC CATGGGACCC CGAGGGCTGC CGGGGCCCCC AGGTCCCCCA
    GGACCACCAG GGCTGGGCTA CGAGGCTGGA TTTGGTGACA TGGAGGGCTC GGGGCTGCCA
    TTTGCTGCCA GCTCCCCTGG ACCGCCTGGC CCCGAAGGAC CACAGGGGGT GCCTGGACTG
    CAGGGGGTAA AGGGGGAGGT CGGCAGCCCC GGGCAGCCCG GCCTGCCAGG GCCGAAGGGA
    GATGCTGGTG TGCCTGGCGT GGATGGCCGC CCTGGCCTGG AGGGCTTCCC CGGACCACAG
    GGACCCAAAG GTGACAGAGG CAGTCCCGGA GAGAAGGGTG AGCGAGGTCA AGATGGTGTG
    GGGCTGCCTG GCCCCCCTGG TCCACCTGGT CCCCCCGGAC AGGTTGTCGC TGTCTCAAAT
    AAAGATAAGT CTTTGGTGGC TTTGCCTGGT CCGGAGGGCA GACAGGGCCG CGCCGGCTTC
    CCGGGCCCAG TGGGACCGAA AGGAGATCAG GGGCCGTCTG GTCCCCAGGG CCCCCCGGGG
    TTGAAGGGGG AGAAGGGGGA GCCCGGTGTC ATCATCAGCC CCGATGGGAC GGTGGTCACT
    GCACAGGTGA AAGGAGAAAA GGGGGAGCCA GGGCCGCAGG GACCGATGGG ACCTTCAGGT
    CCCCAGGGAC GAGGAGGGAT GAAGGGAGAG ATTGGCTTTC CAGGAAGACC TGGACGTCCT
    GGCATGAACG GGCTGAAGGG TGAGAAGGGT GACCCCGCGG ATGTCACTAG CCTGCTGGGC
    TTGAGGGGTC CCCCAGGACC CCCAGGGCCC CCAGGGCCCC CTGGGCCACC CGGCAGCATA
    GTCTACGACA GCAGCAATGC CTTCAGTGAC TCTGGCCATC CCGTGCTGCC AGCTTTCCCC
    GGTTTCCACC AGTTTCAAGG ACAAAAGGGA GAGAAAGGTG ACGCCGGAGC CCCAGGACCC
    CCAGGCCACT TTGCCTACGA CCCGAGTCGC TTTGGTGCCA ACCTGCAGGG TGACAAGGGG
    GATCCGGGTC CCAAGGGTGA GAAGGGCGAG CCAGGCAGCA CCCCACTCTA TGGTCCCAGT
    GTCTCGGGGC TGCCAGGGCC TCCAGGGCCC CAGGGCTACC CTGGGCTGCC GGGTCCCAAG
    GGGGACAGTA TCGTTGGGCC ACCAGGGCCT CCTGGACCTC AGGGCCCCCC CGGGATTGGG
    TATGAGGGGC GGCAAGGCCC CCCTGGCCCT CCTGGGCCCC CCGGTCAACC CTCCTTCCCA
    GGCCCCCACC GACAAGCTAT CAGCATCCCT GGGCCCCCCG GACCACCGGG GCCCCCTGGA
    CCACCAGGCA CCGGCGGGAC GTCCTTGGGG CTGCACGCCC TGCCGAGCTA CCAGGCCATG
    CTGAGTGCTG CCCAGGAGCT GCCCGAGGGA GGGCTCGTCT TCCTCACCGA CCGGCAAGAG
    CTCTACGTCC GCCTGCGCGG GGGCTTCCGC CGGGTGCTGC TGGAGGAGCA CACCCTGATC
    CCCAGCTCAG CTCTGGACAA CGAGGTGTAC GACAAGCCCC CCAGAGTTCA CTATGCTGGC
    CCCCAGCCCC CGCTCCAGCC CCGCGGACCC CTGCATCCCC TTCGCAACCA CAGCCCACCG
    CCCACCACCC GCCCCTGGCA CAGGGACGAG GTGGTGGCCA ACCAGCACCG CCTGCCAGAG
    CAACCCCTTC TCCACCACCA GCACGAGCTC CTCAACAGCT ACTACATCCA CCGCCGGCCG
    GACCCGGCCC CCGTGGCCGC CCACGTGCAC CAGGACTTCC AGCCTGCCCT TCACCTGGTG
    GCCCTGAACG CCCCGCTGAG TGGCAGCATG CGTGGCATCC GGGGTGCGGA CTTCCAGTGC
    TTCCAGCAAG CCCGGCAGGT TGGGCTGGCC GGCACCTTCC GTGCCTTCCT CTCCTCCCGC
    CTGCAGGACC TCTACAGCAT CGTGCGCCGG GCCGACCGCG CCGCTGTGCC CATCGTCAAC
    CTCCGGGACG AAGTGCTCTT CAGTAACTGG GAAGCCCTTT TCACGGGCAA CGGGGCCCCA
    CTGCGAGCTG GCACCCGCAT CCTCTCCTTC GACGGCCGGG ATGTCCTGCA GGATGCAGGA
    TGGCCACAGA AGAGCATTTG GCACGGCTCA GACGCCAAGG GCCGGCGCTT GCCTGAGAGC
    TACTGCGAGA CGTGGCGGAC GGAGGAGCAC GCTGTCACCG GCCAGGCTTC CTCCCTGGGC
    TCGGGCAAGC TGCTGGAGCA GGTGGCCAGC AGCTGCCAGC ACACCTTCAT CGTCCTCTGC
    ATTGAGAACA GCTTCATGAC CGCCGCCAAA AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70350
    Clone ID Related Accession (Same CDS sequence) XM_014949234.1
    Accession Version XM_014949234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(XVIII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGCCCCCC TTCCTCTTCG CTCGGAGCTC CTGCTCCTCC TGGCTTGCTG CTTCTCCTCC 
    TCCCAACCGC AGCTCCTGGA CTGGATTTGG GGCAGCACGA AGCCCACGGG AAGCCCAGCG
    ACATCCAGCA TGGGAACCAC AGGGCTGGAG CCACTCACCT CGGCGGCAGC TGCCACCCAA
    AGCACGTCCC CAGGATCGTG GGGTGTCCGG GTGGTGGAGG ATGTCACCAC CCTGCAGCTC
    CAGGAGCCGG ATCCCAACAC AGCAGCACCT GCAGGTGAGG GGACCACGGC CAAAACCAGC
    AAGCAGCGGG ATGGGAATGC CACGGGGCTG GTGAAGAGCA CGGCGTGGCC AAGCATCGCT
    CCTCCCCGCG GTGTGTCACC TGCCCCTGGC CAGGATGCGG CCAGCAGCTC CACCGAGGAC
    CTGCAGGTCC TGGGGACAGG GACCACCAAG GACCCGCAGC TCCTGGGGAT GGGGGACACC
    GAGGATCTGC AGATCCTGGG GACAGGGACT ACCAAGGACC CGCACCTCCT GGGGACAGGG
    ACCACCAAGG ACCTGCAGTT CCTGGGGATG GAGGACACTG AGGATCTGCA TCTCCTGGGG
    ACAGGGACCA CCGAGGACCC ACAGTTCCTG GGCACAGGGA CCACCAAGGA CCCGCAGTTC
    CTGGGGACAG GGATCACCAA GGACCCACAG ATCCTGGGAA CAGGGACTAC CAAGGACCCG
    CAGTTCCTGG GGACAGGGAC CACCGAGGAC CTGCAGCTCC TGGGGACAGG GACCACCAAG
    GACCCACAGA TCCTGGGAAC AGGGACTACC AAGGACCCGC AGTTCCTGGG GACAGGGACC
    ACCAAGGACC CGCAGCTCCT GGGGATGGAG GACACTGAGG ATTTGCAGCT CCTGGGGACA
    GGGACCACCA AGGACCCACA GTTCCTGGGG ACAGGGACCA CCGAGGACCC GCAGTTCCTG
    GGGACAGGAA CCACCGAGGA CCCGCAGTTC CTGGGGATGG GGGACACGGA GGATCTGCAG
    CTCCAGGGGA CAGGGACCCC CCCAGCAACG AGGACACAGG TGTCCTTGCA GAGACCGTCA
    GGTCCTTGGC CAGGTAGTGC CCCTTTTCCT TTCCCAGCAG GTGACAGGAC CCATGAGCAG
    GCAGTGCCAC CCCAGGACCC CACGGTGCCA CCTCCCCGAC ACAGCCACGG AGCATCACCT
    GCCTCCCTGA GCCCGCGGCG CAGCACCGGC CGCTGGGGGC TGGGGCTGAG CCCCCTGAGA
    GCAGCCAGCC CTGCGGATCC TCCTCCTGCC AACACCTCCC GTCCTCTTAA GTTCGATTTA
    CTAACTGCTG CTTCACGACT CTACAGCGGT GGTGGCACGG GGCTGGTGGC CTTCTCGCCC
    GGACTGATGC CGCCGGCCGG CCGGTGCCTG CCCGTCCCGA CTCCCCTGTC CTTCTGCAGC
    GTGCTGGGCA CCCAGCACGT CAGGGTCCCC AATTACCTCC GCCACGGCAG CGAGGGGGAA
    ATTCGGGCGG CTTTGCGTGA GTGGGAGGGG CTGCTGGAAT CGCGGTGCCA TCGCTACCTG
    GAGTGGTTTC TCTGCTTGCT CCTGCTGCCC GGGTGTAGCC CTTCGGTCCC GGTAACCCCC
    CCGCCGTGCC AGGGCTTCTG CGAGGCCGTC AGAGACCTGT GTTGGATGCA CTTGGCCGGC
    GGGCGCCTGC CTCTGCCTTG TGATGCTCTC CCTCGGGAGG ACGGAGGGTA TTCTTGTGTC
    TTTATTAATG CTTCTGCAGA GAACCTGAGC ACCGAGGTCA GCCTCTTAGA GCTGATCGGA
    GACCCCCCGC CGGAGGAGAT CCTCAAAATT TACGGCCCCG ACAACAATCC CGGCTACGTC
    TTCGGCCCCA ATGCCAACAC AGGCCAGGTG GCCCGGTACC ACCTGCCGAG CCCTTTCTAC
    CGGGACTTCT CCATCCTGTT CCACATCCAG CCCACCACAC CCAGGGCTGG TGTGCTCTTT
    GCCATCACAG ACTCCTCGCA GAGCATCATC TATGTGGGCA TCAAGCTCTC AGAGCTGCGG
    GCGGGCAAGC AGCAGATCAT CTTCTACTAC ACAGAGCCGG GCTCACCCAG CTCATATGCA
    GCAGCCACCT TCACCGTGCC CACCTTGCTC AACCAGTGGA CGCGCTTCGC CATCAGCGTG
    GAGGAGGACG AAGTTGTTCT CTACCTGGAC TGTGAGGAGC ACGAGCGGGT CCGTTTCGAG
    CGCTCCCCGG ATGAGATGGA GCTGGAAGAT GGCTCCGGGC TCTTTGTTGC TCAGGCTGGA
    GGGGCTGACC CAGATAAATA TCAGGGGGTG ATCGCCGATT TGAAGCTGCG AGGGGACCCA
    CGGGCGGCCG AGCGCCAGTG CGAGGAAGAG GAGGATGACA CCGAGGAGGT CTCTGGCGAT
    TTTGGCAGCG GGGCAGAAGG TGGACACCAA CCCTCAGGGA AGGACAGGGG TGTTCCAGGG
    CTGGTGGATG CTGTCCCTGT CACCTCTCCT CCTGTGGCAG CGGGTGGTGG GATGAGGAGC
    GGTGGAGATT CCCCACAGCA AGCTGAGAGG ACCAGAGCAG AGGAGACACC GCAGGTCTCC
    ACGGGAGGCA CCGGCCCCAA AGGTGAAAAA GGGGAGAAGG GCGAGCGTGG CCTGAAAGGG
    GACTCGGGGA CCGGCAGCGT CGTGGGGATG GGCAGTGTCA AGGGTGAAAA GGGGGAGAAA
    GGAGAGCTGG GTGTTAAGGG CAGCGCTGGA TTTGGCTACC CGGGCTCCAA GGGCCAGAAA
    GGAGAGCCAG GTAACCCCGG CCCGCCGGGG ACCCTCTCGC GGCATTCTGA TGGCTCGGTG
    GTGGAGCAGG TCACCGGTCC CCCAGGGCCA CCAGGGAAGG ATGGAGCCCC TGGCAGGGAT
    GGCGAGCCCG GTGATCCTGG CGAGGATGGC AAACCTGGCG ATATGGGGCC CCAGGGGTTC
    CCCGGGATGC CGGGGGAGCC TGGTCTGAAG GGGGAGAAGG GTGACCCAGG CGTAGGGCCA
    AGGGGACCCC CTGGACCACC CGGTCCTCCA GGACCTCCAG GACCATCATC CAAGCCTGAC
    AAGCTGACCT TCATCGACAT GGAGGGCTCT GGCTTTGGCA GTGACCTGGA GACCCTGCGG
    GGTCCGCGAG GGCCACCTGG TCCCCCAGGG CCACCCGGTG TGCCCGGTTT GCCAGGTGAG
    CCGGGACGGT TTGGGATGAA CCGCACAGAC CTGCAGGGAC TGCCAGGGCT GCCGGGCAGG
    GACGGGACCC CTGGGTCCTC AGGGCCAGCG GGTCCTCAGG GTCCTCCTGG AAGAGATGGG
    GCACCTGGGC AGCCAGGGCC CAAAGGAGAG CAGGGTGATG TGGGTGACCT TGGCCTCCCT
    GGCGCACCAG GACCCAAGGG CAGCAAGGGA GAAACAGGAC CAGCAGGAGC CCCAGGAGAA
    ATGGGGTTAG CTGGCCTTCC TGGGCCCATG GGACCCCGAG GGCTGCCGGG GCCCCCAGGT
    CCCCCAGGAC CACCAGGGCT GGGCTACGAG GCTGGATTTG GTGACATGGA GGGCTCGGGG
    CTGCCATTTG CTGCCAGCTC CCCTGGACCG CCTGGCCCCG AAGGACCACA GGGGGTGCCT
    GGACTGCAGG GGGTAAAGGG GGAGGTCGGC AGCCCCGGGC AGCCCGGCCT GCCAGGGCCG
    AAGGGAGATG CTGGTGTGCC TGGCGTGGAT GGCCGCCCTG GCCTGGAGGG CTTCCCCGGA
    CCACAGGGAC CCAAAGGTGA CAGAGGCAGT CCCGGAGAGA AGGGTGAGCG AGGTCAAGAT
    GGTGTGGGGC TGCCTGGCCC CCCTGGTCCA CCTGGTCCCC CCGGACAGGT TGTCGCTGTC
    TCAAATAAAG ATAAGTCTTT GGTGGCTTTG CCTGGTCCGG AGGGCAGACA GGGCCGCGCC
    GGCTTCCCGG GCCCAGTGGG ACCGAAAGGA GATCAGGGGC CGTCTGGTCC CCAGGGCCCC
    CCGGGGTTGA AGGGGGAGAA GGGGGAGCCC GGTGTCATCA TCAGCCCCGA TGGGACGGTG
    GTCACTGCAC AGGTGAAAGG AGAAAAGGGG GAGCCAGGGC CGCAGGGACC GATGGGACCT
    TCAGGTCCCC AGGGACGAGG AGGGATGAAG GGAGAGATTG GCTTTCCAGG AAGACCTGGA
    CGTCCTGGCA TGAACGGGCT GAAGGGTGAG AAGGGTGACC CCGCGGATGT CACTAGCCTG
    CTGGGCTTGA GGGGTCCCCC AGGACCCCCA GGGCCCCCAG GGCCCCCTGG GCCACCCGGC
    AGCATAGTCT ACGACAGCAG CAATGCCTTC AGTGACTCTG GCCATCCCGT GCTGCCAGCT
    TTCCCCGGTT TCCACCAGTT TCAAGGACAA AAGGGAGAGA AAGGTGACGC CGGAGCCCCA
    GGACCCCCAG GCCACTTTGC CTACGACCCG AGTCGCTTTG GTGCCAACCT GCAGGGTGAC
    AAGGGGGATC CGGGTCCCAA GGGTGAGAAG GGCGAGCCAG GCAGCACCCC ACTCTATGGT
    CCCAGTGTCT CGGGGCTGCC AGGGCCTCCA GGGCCCCAGG GCTACCCTGG GCTGCCGGGT
    CCCAAGGGGG ACAGTATCGT TGGGCCACCA GGGCCTCCTG GACCTCAGGG CCCCCCCGGG
    ATTGGGTATG AGGGGCGGCA AGGCCCCCCT GGCCCTCCTG GGCCCCCCGG TCAACCCTCC
    TTCCCAGGCC CCCACCGACA AGCTATCAGC ATCCCTGGGC CCCCCGGACC ACCGGGGCCC
    CCTGGACCAC CAGGCACCGG CGGGACGTCC TTGGGGCTGC ACGCCCTGCC GAGCTACCAG
    GCCATGCTGA GTGCTGCCCA GGAGCTGCCC GAGGGAGGGC TCGTCTTCCT CACCGACCGG
    CAAGAGCTCT ACGTCCGCCT GCGCGGGGGC TTCCGCCGGG TGCTGCTGGA GGAGCACACC
    CTGATCCCCA GCTCAGCTCT GGACAACGAG GTGTACGACA AGCCCCCCAG AGTTCACTAT
    GCTGGCCCCC AGCCCCCGCT CCAGCCCCGC GGACCCCTGC ATCCCCTTCG CAACCACAGC
    CCACCGCCCA CCACCCGCCC CTGGCACAGG GACGAGGTGG TGGCCAACCA GCACCGCCTG
    CCAGAGCAAC CCCTTCTCCA CCACCAGCAC GAGCTCCTCA ACAGCTACTA CATCCACCGC
    CGGCCGGACC CGGCCCCCGT GGCCGCCCAC GTGCACCAGG ACTTCCAGCC TGCCCTTCAC
    CTGGTGGCCC TGAACGCCCC GCTGAGTGGC AGCATGCGTG GCATCCGGGG TGCGGACTTC
    CAGTGCTTCC AGCAAGCCCG GCAGGTTGGG CTGGCCGGCA CCTTCCGTGC CTTCCTCTCC
    TCCCGCCTGC AGGACCTCTA CAGCATCGTG CGCCGGGCCG ACCGCGCCGC TGTGCCCATC
    GTCAACCTCC GGGACGAAGT GCTCTTCAGT AACTGGGAAG CCCTTTTCAC GGGCAACGGG
    GCCCCACTGC GAGCTGGCAC CCGCATCCTC TCCTTCGACG GCCGGGATGT CCTGCAGGAT
    GCAGGATGGC CACAGAAGAG CATTTGGCAC GGCTCAGACG CCAAGGGCCG GCGCTTGCCT
    GAGAGCTACT GCGAGACGTG GCGGACGGAG GAGCACGCTG TCACCGGCCA GGCTTCCTCC
    CTGGGCTCGG GCAAGCTGCT GGAGCAGGTG GCCAGCAGCT GCCAGCACAC CTTCATCGTC
    CTCTGCATTG AGAACAGCTT CATGACCGCC GCCAAAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804720.1
    CDS102..5843
    Translation

    Target ORF information:

    RefSeq Version XM_014949234.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen, type XVIII, alpha 1 (COL18A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGCCCCCC TTCCTCTTCG CTCGGAGCTC CTGCTCCTCC TGGCTTGCTG CTTCTCCTCC 
    TCCCAACCGC AGCTCCTGGA CTGGATTTGG GGCAGCACGA AGCCCACGGG AAGCCCAGCG
    ACATCCAGCA TGGGAACCAC AGGGCTGGAG CCACTCACCT CGGCGGCAGC TGCCACCCAA
    AGCACGTCCC CAGGATCGTG GGGTGTCCGG GTGGTGGAGG ATGTCACCAC CCTGCAGCTC
    CAGGAGCCGG ATCCCAACAC AGCAGCACCT GCAGGTGAGG GGACCACGGC CAAAACCAGC
    AAGCAGCGGG ATGGGAATGC CACGGGGCTG GTGAAGAGCA CGGCGTGGCC AAGCATCGCT
    CCTCCCCGCG GTGTGTCACC TGCCCCTGGC CAGGATGCGG CCAGCAGCTC CACCGAGGAC
    CTGCAGGTCC TGGGGACAGG GACCACCAAG GACCCGCAGC TCCTGGGGAT GGGGGACACC
    GAGGATCTGC AGATCCTGGG GACAGGGACT ACCAAGGACC CGCACCTCCT GGGGACAGGG
    ACCACCAAGG ACCTGCAGTT CCTGGGGATG GAGGACACTG AGGATCTGCA TCTCCTGGGG
    ACAGGGACCA CCGAGGACCC ACAGTTCCTG GGCACAGGGA CCACCAAGGA CCCGCAGTTC
    CTGGGGACAG GGATCACCAA GGACCCACAG ATCCTGGGAA CAGGGACTAC CAAGGACCCG
    CAGTTCCTGG GGACAGGGAC CACCGAGGAC CTGCAGCTCC TGGGGACAGG GACCACCAAG
    GACCCACAGA TCCTGGGAAC AGGGACTACC AAGGACCCGC AGTTCCTGGG GACAGGGACC
    ACCAAGGACC CGCAGCTCCT GGGGATGGAG GACACTGAGG ATTTGCAGCT CCTGGGGACA
    GGGACCACCA AGGACCCACA GTTCCTGGGG ACAGGGACCA CCGAGGACCC GCAGTTCCTG
    GGGACAGGAA CCACCGAGGA CCCGCAGTTC CTGGGGATGG GGGACACGGA GGATCTGCAG
    CTCCAGGGGA CAGGGACCCC CCCAGCAACG AGGACACAGG TGTCCTTGCA GAGACCGTCA
    GGTCCTTGGC CAGGTAGTGC CCCTTTTCCT TTCCCAGCAG GTGACAGGAC CCATGAGCAG
    GCAGTGCCAC CCCAGGACCC CACGGTGCCA CCTCCCCGAC ACAGCCACGG AGCATCACCT
    GCCTCCCTGA GCCCGCGGCG CAGCACCGGC CGCTGGGGGC TGGGGCTGAG CCCCCTGAGA
    GCAGCCAGCC CTGCGGATCC TCCTCCTGCC AACACCTCCC GTCCTCTTAA GTTCGATTTA
    CTAACTGCTG CTTCACGACT CTACAGCGGT GGTGGCACGG GGCTGGTGGC CTTCTCGCCC
    GGACTGATGC CGCCGGCCGG CCGGTGCCTG CCCGTCCCGA CTCCCCTGTC CTTCTGCAGC
    GTGCTGGGCA CCCAGCACGT CAGGGTCCCC AATTACCTCC GCCACGGCAG CGAGGGGGAA
    ATTCGGGCGG CTTTGCGTGA GTGGGAGGGG CTGCTGGAAT CGCGGTGCCA TCGCTACCTG
    GAGTGGTTTC TCTGCTTGCT CCTGCTGCCC GGGTGTAGCC CTTCGGTCCC GGTAACCCCC
    CCGCCGTGCC AGGGCTTCTG CGAGGCCGTC AGAGACCTGT GTTGGATGCA CTTGGCCGGC
    GGGCGCCTGC CTCTGCCTTG TGATGCTCTC CCTCGGGAGG ACGGAGGGTA TTCTTGTGTC
    TTTATTAATG CTTCTGCAGA GAACCTGAGC ACCGAGGTCA GCCTCTTAGA GCTGATCGGA
    GACCCCCCGC CGGAGGAGAT CCTCAAAATT TACGGCCCCG ACAACAATCC CGGCTACGTC
    TTCGGCCCCA ATGCCAACAC AGGCCAGGTG GCCCGGTACC ACCTGCCGAG CCCTTTCTAC
    CGGGACTTCT CCATCCTGTT CCACATCCAG CCCACCACAC CCAGGGCTGG TGTGCTCTTT
    GCCATCACAG ACTCCTCGCA GAGCATCATC TATGTGGGCA TCAAGCTCTC AGAGCTGCGG
    GCGGGCAAGC AGCAGATCAT CTTCTACTAC ACAGAGCCGG GCTCACCCAG CTCATATGCA
    GCAGCCACCT TCACCGTGCC CACCTTGCTC AACCAGTGGA CGCGCTTCGC CATCAGCGTG
    GAGGAGGACG AAGTTGTTCT CTACCTGGAC TGTGAGGAGC ACGAGCGGGT CCGTTTCGAG
    CGCTCCCCGG ATGAGATGGA GCTGGAAGAT GGCTCCGGGC TCTTTGTTGC TCAGGCTGGA
    GGGGCTGACC CAGATAAATA TCAGGGGGTG ATCGCCGATT TGAAGCTGCG AGGGGACCCA
    CGGGCGGCCG AGCGCCAGTG CGAGGAAGAG GAGGATGACA CCGAGGAGGT CTCTGGCGAT
    TTTGGCAGCG GGGCAGAAGG TGGACACCAA CCCTCAGGGA AGGACAGGGG TGTTCCAGGG
    CTGGTGGATG CTGTCCCTGT CACCTCTCCT CCTGTGGCAG CGGGTGGTGG GATGAGGAGC
    GGTGGAGATT CCCCACAGCA AGCTGAGAGG ACCAGAGCAG AGGAGACACC GCAGGTCTCC
    ACGGGAGGCA CCGGCCCCAA AGGTGAAAAA GGGGAGAAGG GCGAGCGTGG CCTGAAAGGG
    GACTCGGGGA CCGGCAGCGT CGTGGGGATG GGCAGTGTCA AGGGTGAAAA GGGGGAGAAA
    GGAGAGCTGG GTGTTAAGGG CAGCGCTGGA TTTGGCTACC CGGGCTCCAA GGGCCAGAAA
    GGAGAGCCAG GTAACCCCGG CCCGCCGGGG ACCCTCTCGC GGCATTCTGA TGGCTCGGTG
    GTGGAGCAGG TCACCGGTCC CCCAGGGCCA CCAGGGAAGG ATGGAGCCCC TGGCAGGGAT
    GGCGAGCCCG GTGATCCTGG CGAGGATGGC AAACCTGGCG ATATGGGGCC CCAGGGGTTC
    CCCGGGATGC CGGGGGAGCC TGGTCTGAAG GGGGAGAAGG GTGACCCAGG CGTAGGGCCA
    AGGGGACCCC CTGGACCACC CGGTCCTCCA GGACCTCCAG GACCATCATC CAAGCCTGAC
    AAGCTGACCT TCATCGACAT GGAGGGCTCT GGCTTTGGCA GTGACCTGGA GACCCTGCGG
    GGTCCGCGAG GGCCACCTGG TCCCCCAGGG CCACCCGGTG TGCCCGGTTT GCCAGGTGAG
    CCGGGACGGT TTGGGATGAA CCGCACAGAC CTGCAGGGAC TGCCAGGGCT GCCGGGCAGG
    GACGGGACCC CTGGGTCCTC AGGGCCAGCG GGTCCTCAGG GTCCTCCTGG AAGAGATGGG
    GCACCTGGGC AGCCAGGGCC CAAAGGAGAG CAGGGTGATG TGGGTGACCT TGGCCTCCCT
    GGCGCACCAG GACCCAAGGG CAGCAAGGGA GAAACAGGAC CAGCAGGAGC CCCAGGAGAA
    ATGGGGTTAG CTGGCCTTCC TGGGCCCATG GGACCCCGAG GGCTGCCGGG GCCCCCAGGT
    CCCCCAGGAC CACCAGGGCT GGGCTACGAG GCTGGATTTG GTGACATGGA GGGCTCGGGG
    CTGCCATTTG CTGCCAGCTC CCCTGGACCG CCTGGCCCCG AAGGACCACA GGGGGTGCCT
    GGACTGCAGG GGGTAAAGGG GGAGGTCGGC AGCCCCGGGC AGCCCGGCCT GCCAGGGCCG
    AAGGGAGATG CTGGTGTGCC TGGCGTGGAT GGCCGCCCTG GCCTGGAGGG CTTCCCCGGA
    CCACAGGGAC CCAAAGGTGA CAGAGGCAGT CCCGGAGAGA AGGGTGAGCG AGGTCAAGAT
    GGTGTGGGGC TGCCTGGCCC CCCTGGTCCA CCTGGTCCCC CCGGACAGGT TGTCGCTGTC
    TCAAATAAAG ATAAGTCTTT GGTGGCTTTG CCTGGTCCGG AGGGCAGACA GGGCCGCGCC
    GGCTTCCCGG GCCCAGTGGG ACCGAAAGGA GATCAGGGGC CGTCTGGTCC CCAGGGCCCC
    CCGGGGTTGA AGGGGGAGAA GGGGGAGCCC GGTGTCATCA TCAGCCCCGA TGGGACGGTG
    GTCACTGCAC AGGTGAAAGG AGAAAAGGGG GAGCCAGGGC CGCAGGGACC GATGGGACCT
    TCAGGTCCCC AGGGACGAGG AGGGATGAAG GGAGAGATTG GCTTTCCAGG AAGACCTGGA
    CGTCCTGGCA TGAACGGGCT GAAGGGTGAG AAGGGTGACC CCGCGGATGT CACTAGCCTG
    CTGGGCTTGA GGGGTCCCCC AGGACCCCCA GGGCCCCCAG GGCCCCCTGG GCCACCCGGC
    AGCATAGTCT ACGACAGCAG CAATGCCTTC AGTGACTCTG GCCATCCCGT GCTGCCAGCT
    TTCCCCGGTT TCCACCAGTT TCAAGGACAA AAGGGAGAGA AAGGTGACGC CGGAGCCCCA
    GGACCCCCAG GCCACTTTGC CTACGACCCG AGTCGCTTTG GTGCCAACCT GCAGGGTGAC
    AAGGGGGATC CGGGTCCCAA GGGTGAGAAG GGCGAGCCAG GCAGCACCCC ACTCTATGGT
    CCCAGTGTCT CGGGGCTGCC AGGGCCTCCA GGGCCCCAGG GCTACCCTGG GCTGCCGGGT
    CCCAAGGGGG ACAGTATCGT TGGGCCACCA GGGCCTCCTG GACCTCAGGG CCCCCCCGGG
    ATTGGGTATG AGGGGCGGCA AGGCCCCCCT GGCCCTCCTG GGCCCCCCGG TCAACCCTCC
    TTCCCAGGCC CCCACCGACA AGCTATCAGC ATCCCTGGGC CCCCCGGACC ACCGGGGCCC
    CCTGGACCAC CAGGCACCGG CGGGACGTCC TTGGGGCTGC ACGCCCTGCC GAGCTACCAG
    GCCATGCTGA GTGCTGCCCA GGAGCTGCCC GAGGGAGGGC TCGTCTTCCT CACCGACCGG
    CAAGAGCTCT ACGTCCGCCT GCGCGGGGGC TTCCGCCGGG TGCTGCTGGA GGAGCACACC
    CTGATCCCCA GCTCAGCTCT GGACAACGAG GTGTACGACA AGCCCCCCAG AGTTCACTAT
    GCTGGCCCCC AGCCCCCGCT CCAGCCCCGC GGACCCCTGC ATCCCCTTCG CAACCACAGC
    CCACCGCCCA CCACCCGCCC CTGGCACAGG GACGAGGTGG TGGCCAACCA GCACCGCCTG
    CCAGAGCAAC CCCTTCTCCA CCACCAGCAC GAGCTCCTCA ACAGCTACTA CATCCACCGC
    CGGCCGGACC CGGCCCCCGT GGCCGCCCAC GTGCACCAGG ACTTCCAGCC TGCCCTTCAC
    CTGGTGGCCC TGAACGCCCC GCTGAGTGGC AGCATGCGTG GCATCCGGGG TGCGGACTTC
    CAGTGCTTCC AGCAAGCCCG GCAGGTTGGG CTGGCCGGCA CCTTCCGTGC CTTCCTCTCC
    TCCCGCCTGC AGGACCTCTA CAGCATCGTG CGCCGGGCCG ACCGCGCCGC TGTGCCCATC
    GTCAACCTCC GGGACGAAGT GCTCTTCAGT AACTGGGAAG CCCTTTTCAC GGGCAACGGG
    GCCCCACTGC GAGCTGGCAC CCGCATCCTC TCCTTCGACG GCCGGGATGT CCTGCAGGAT
    GCAGGATGGC CACAGAAGAG CATTTGGCAC GGCTCAGACG CCAAGGGCCG GCGCTTGCCT
    GAGAGCTACT GCGAGACGTG GCGGACGGAG GAGCACGCTG TCACCGGCCA GGCTTCCTCC
    CTGGGCTCGG GCAAGCTGCT GGAGCAGGTG GCCAGCAGCT GCCAGCACAC CTTCATCGTC
    CTCTGCATTG AGAACAGCTT CATGACCGCC GCCAAAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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