LOC106900570 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106900570
Entrez Gene ID	106900570
Full Name	myosin heavy chain, skeletal muscle, adult-like
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014962662.1	XP_014818148.1	myosin heavy chain, skeletal muscle, adult-like

Pathways from BioSystems
Gene Ontology

The following LOC106900570 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106900570 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa79991	XM_014962662.1 Latest version!	Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106900570), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106900570 ( XM_014962662.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014818148.1 Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106900570), mRNA.

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CloneID	OCa79991
Clone ID Related Accession (Same CDS sequence)	XM_014962662.1
Accession Version	XM_014962662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	myosin heavy chain, skeletal muscle, adult-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015092761.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTG CAGACTCTGA GATGGCTGCC TTTGGGGAGG CGGCTCCTTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATTGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGAC ATCGGTCTTT 
GTGGCACATC CTAAGGAATC CTTTGTGAAA GGAACAATCC AGAGCAGAGA ATCAGGGAAG 
GTCACCGTCA AGACTGAAGG TGGAGAGACC CTGACTGTGA AGGAAGATCA AATCTTCTCC 
ATGAACCCTC CCAAGTATGA TAAAATCGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGTCTCTTCT GCGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA GAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAT CGCGAGAACC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CCGGGAAGAC TGTGAACACA AAGCGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCCAGTG GGGAGAAGAA GAAGGAGGAG ACATCTGGCA AAATGCAGGG AACGCTTGAG 
GATCAAATCA TCAGCGCCAA CCCACTGCTG GAGGCCTTTG GAAATGCCAA GACCGTGAGG 
AACGACAACT CCTCACGCTT TGGCAAATTC ATCAGAATCC ACTTTGGAGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACCTATCTG CTGGAGAAGT CCAGAGTCAC TTTCCAGCTT 
AAGGCAGAAA GAAGCTACCA CATATTTTAT CAGATCACCT CCAACAAGAA GCCGGAGTTA 
ATTGAAATGC TCCTCATCAC CACCAACCCA TATGACTACC CATTTGTAAG TCAAGGTGAG 
ATCACTGTTC CCAGCATTGA TGACAAGGAG GAGCTGATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCTCTACGGA TGAAAAGACA GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ACCTGAAGTT CAAGCAGAAA CCAAGAGAGG AACAGGCAGA GCCGGAAGGC 
ACAGAAGTTG CTGACAAGGC TGCCTACCTG ATGGGTCTGA ATTCAGCTGA CATGCTCAAG 
GCCCTCTGCT ACCCCAGAGT CAAGGTGGGG AATGAATATG TGACCAAGGG TCAAACTGCA 
CAGCAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCCTG 
TGGATGGTTG TTCGTATCAA CCAACAGCTG GATACCAAGC AGCCCAGACA GTACTTCATT 
GGTGTCCTGG ACATTGCTGG CTTCGAGATC TTTGATTTCA ACAGCTTTGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGAATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA TCAGCATCTG 
GGCAAGTCCA GTAACTTCCA GAAGCCCAAA CCTACCAAAG GCAAGGTTGA GGCCCACTTC 
TCCCTGATTC ACTATGCTGG CACGGTGGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG TTGTACCAGA AATCATCATT GAAGACACTG 
GCCTTACTCT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTGCCGCTGG TGGTGGCAAA 
AAGGGTGCCA AGAAGAAGGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAT 
TTAAACAAGC TGATGACCAA TCTGCGAAGC ACCCATCCTC ATTTTGTACG CTGCATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAAC TGGTGCTTCA CCAGCTGCGG 
TGTAACGGCG TGCTGGAAGG CATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA TTAAATGCAA GTGCTATCCC GGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTTCTTGGAT CAATTGATAT AGACCACACT 
CAATACAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGATAGG CCTCTTGGAA 
GAGATGAGAG ATGAAAAGTT GGCACAGATC ATCACTCGCA CACAGGCAAG GTGCAGGGGC 
TTCCTGATGA GAGTGGAGTA CCAGAGAATG GTGGAGAGGA GGGAGTCCAT CTTCTGCATC 
CAGTACAACA TTCGGGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTC 
AAGATCAAGC CCTTGCTGAA GAGCGCCGAA TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AAACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAACTGGT GCAGGAAAAA AATGACCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCTT TGGCTGATGC TGAGGAAAGG TGCGACCAGC TCATTAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTTAAAGA AGTGACTGAA AGGGCAGAGG ATGAGGAAGA AATTAACGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAGCTGAA GAAAGACATT 
GACGATCTTG AGCTAACACT GGCCAAGGTT GAGAAGGAAA AGCATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCAGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAG ACCAAGCTGG AGCAGCAAGT GGACGATCTG 
GAAGGGTCCC TGGAGCAGGA GAAGAAGCTG CGCATGGACC TTGAGAGAGC TAAGAGGAAA 
CTCGAAGGAG ACCTGAAGCT GGCCCATGAC AGCATAATGG ATTTGGAAAA TGATAAGCAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAGATC 
GAGGATGAAC AACTTCTGGG CATCCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCGCGT 
ATTGAGGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CATCGTGCTG ACCTCTCGAG GGAGCTGGAG GAGATCAGCG AGCGCCTGGA AGAAGCAGGA 
GGGGCCACTG CAGCTCAGAT TGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGTCGCGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCCGC CCTGCGGAAG 
AAGCACGCGG ACAGCACGGC CGAGCTCGGG GAGCAGATCG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGTAAC 
ATGGAGTCTG TCTCCAAAGC CAAGGCAAAC TTGGAGAAGA TGTGCCGCAC TCTGGAAGAC 
CAATTAAATG AATTTAAAAC AAAGGAGGAG CAGAATCAGC GCATGATCAG CGATCTCAGT 
GCTCAAAGAG CTCGTCTGCA GACAGAATCT GGGGAATATG CACGCCAGGC TGAGGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCTAGAGGG AAACAGGCTT TCACCCAGCA GATTGAAGAA 
TTGAAGAGGC AACTAGAGGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCTGCTCGCC ACGACTGTGA CTTGCTCCGG GAACAATATG AGGAGGAGCA GGAGGCCAAG 
GGGGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TACGAGACGG ACGCTATTCA GCGCACGGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCAGA GGAACACGTT GAAGCTGTCA ATGCCAAATG TGCCTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAA GTGGAGGACC TGATGATTGA CGTGGAGCGA 
TCAAATGCTG CCTGCGCAGC TCTGGATAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTATGA GGAAACGCAG GCTGAGCTGG AAGCCTCCCA GAAGGAGTCT 
CGCTCTCTCA GCACGGAGCT CTTTAAGATG AAGAATGCCT ATGAGGAGTC CTTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCCGA CCTCACGGAG 
CAGATTGCCG AGGGAGGAAA GGCGATTCAT GAGCTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAAT CTGAACTCCA AGCCTCCCTG GAGGAAGCCG AGGCTTCTCT AGAACACGAA 
GAGGGGAAGA TCCTGCGCCT CCAGCTTGAG CTCAACCAGG TGAAGTCTGA GATTGACAGG 
AAGGTAGCAG AGAAAGATGA GGAGATCGAC CAGCTGAAGA GAAACCACCT CAGAATCGTG 
GAGTCCATGC AGAGCACCCT GGACGCTGAG ATCAGGAGCA GGAATGAAGC CCTGCGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCCAACCGC 
CAGGCTGCAG AGGCACAAAA GAACCTGAGG AACACACAGG GAGTGCTCAA GGATACCCAG 
ATACACTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGCT GCAGGCTGAA GTTGAGGAGC TGCGGGCAGC CCTGGAGCAG 
ACGGAGCGCT CGAGGAAAGT GGCTGAGCAG GAGCTTCTGG ATGCCACTGA ACGTGTGCAG 
CTCCTCCACA CCCAGAACAC CAGCTTAATT AACACCAAGA AGAAGCTGGA AACAGACATC 
ATGCAAATTC AGGGTGAAAT GGAGGATACG ATCCAGGAAG CGCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACAGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAAAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGTCTGG ATGAGGCGGA GCAGCTGGCT CTGAAGGGAG GCAAGAAGCA GCTCCAGAAG 
CTGGAGGCCA GAGTGCGGGA GCTGGAAGGG GAGGTGGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGACC GGAAGAATAT TCTGAGGCTC CAGGATCTGG TGGACAAGCT GCAAACGAAG 
GTGAAATCCT ACAAGAGACA AGCTGAGGAG GCTGAGGAGC TCTCCAACGT CAACCTCTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAAGCCGAGG AGCGGGCTGA CATTGCAGAG 
TCGCAGGTCA ACAAGCTGCG AGCGAAGAGT CGGGAGTTTC ATAGGAAGAT AGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014818148.1
CDS	202..6027
Translation	MASADSEMAAFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAHPKESFVKGTIQSRESGKVTVKTEGGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEETSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEITVPSIDDKEELMATDSAIDILGFSTDEKTAIYKLTGAVMHYGNLKFKQKPREEQAEPEGTEVADKAAYLMGLNSADMLKALCYPRVKVGNEYVTKGQTAQQVHNAVGALAKAVYERMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPTKGKVEAHFSLIHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSLKTLALLFANYGGAEAEAAAGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDIDHTQYKFGHTKVFFKAGLIGLLEEMRDEKLAQIITRTQARCRGFLMRVEYQRMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSEAKRKELEEKMVKLVQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKVKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQLLGIQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKANLEKMCRTLEDQLNEFKTKEEQNQRMISDLSAQRARLQTESGEYARQAEEKDGLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHSLQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSELQASLEEAEASLEHEEGKILRLQLELNQVKSEIDRKVAEKDEEIDQLKRNHLRIVESMQSTLDAEIRSRNEALRLKKKMEGDLNEMEIQLSHANRQAAEAQKNLRNTQGVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEVEELRAALEQTERSRKVAEQELLDATERVQLLHTQNTSLINTKKKLETDIMQIQGEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQLQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQTKVKSYKRQAEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREFHRKIEEEE

Target ORF information:

RefSeq Version	XM_014962662.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106900570), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014962662.1

ORF Insert Sequence:

ATGGCCTCTG CAGACTCTGA GATGGCTGCC TTTGGGGAGG CGGCTCCTTA CCTCCGAAAG 
TCAGAGAAGG AAAGAATTGA GGCCCAGAAC AAGCCTTTTG ATGCCAAGAC ATCGGTCTTT 
GTGGCACATC CTAAGGAATC CTTTGTGAAA GGAACAATCC AGAGCAGAGA ATCAGGGAAG 
GTCACCGTCA AGACTGAAGG TGGAGAGACC CTGACTGTGA AGGAAGATCA AATCTTCTCC 
ATGAACCCTC CCAAGTATGA TAAAATCGAG GACATGGCCA TGATGACCCA CCTCCATGAA 
CCCGCTGTGC TGTACAACCT CAAAGAGCGT TATGCAGCCT GGATGATCTA CACCTACTCG 
GGTCTCTTCT GCGTCACTGT CAACCCCTAC AAGTGGCTGC CGGTGTACAA CCCGGAGGTG 
GTGTTGGCCT ACCGAGGCAA GAAGCGCCAG GAGGCCCCTC CACACATCTT CTCCATCTCT 
GACAACGCCT ATCAGTTCAT GCTGACTGAT CGCGAGAACC AGTCGATCCT GATCACCGGA 
GAATCCGGTG CCGGGAAGAC TGTGAACACA AAGCGTGTCA TCCAGTACTT TGCAACAATT 
GCAGCCAGTG GGGAGAAGAA GAAGGAGGAG ACATCTGGCA AAATGCAGGG AACGCTTGAG 
GATCAAATCA TCAGCGCCAA CCCACTGCTG GAGGCCTTTG GAAATGCCAA GACCGTGAGG 
AACGACAACT CCTCACGCTT TGGCAAATTC ATCAGAATCC ACTTTGGAGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACCTATCTG CTGGAGAAGT CCAGAGTCAC TTTCCAGCTT 
AAGGCAGAAA GAAGCTACCA CATATTTTAT CAGATCACCT CCAACAAGAA GCCGGAGTTA 
ATTGAAATGC TCCTCATCAC CACCAACCCA TATGACTACC CATTTGTAAG TCAAGGTGAG 
ATCACTGTTC CCAGCATTGA TGACAAGGAG GAGCTGATGG CTACAGATAG TGCCATTGAC 
ATCCTGGGCT TCTCTACGGA TGAAAAGACA GCCATCTACA AGCTGACAGG GGCTGTCATG 
CACTACGGGA ACCTGAAGTT CAAGCAGAAA CCAAGAGAGG AACAGGCAGA GCCGGAAGGC 
ACAGAAGTTG CTGACAAGGC TGCCTACCTG ATGGGTCTGA ATTCAGCTGA CATGCTCAAG 
GCCCTCTGCT ACCCCAGAGT CAAGGTGGGG AATGAATATG TGACCAAGGG TCAAACTGCA 
CAGCAGGTGC ACAATGCTGT AGGTGCTCTG GCAAAGGCTG TCTATGAGAG GATGTTCCTG 
TGGATGGTTG TTCGTATCAA CCAACAGCTG GATACCAAGC AGCCCAGACA GTACTTCATT 
GGTGTCCTGG ACATTGCTGG CTTCGAGATC TTTGATTTCA ACAGCTTTGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGAATTGAA TGGACATTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA TCAGCATCTG 
GGCAAGTCCA GTAACTTCCA GAAGCCCAAA CCTACCAAAG GCAAGGTTGA GGCCCACTTC 
TCCCTGATTC ACTATGCTGG CACGGTGGAC TACAACATCA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAACGAAAC TGTCATTGGG TTGTACCAGA AATCATCATT GAAGACACTG 
GCCTTACTCT TTGCCAACTA TGGTGGAGCA GAAGCAGAGG CTGCCGCTGG TGGTGGCAAA 
AAGGGTGCCA AGAAGAAGGG TTCTTCTTTC CAGACTGTCT CAGCTCTTTT CCGGGAGAAT 
TTAAACAAGC TGATGACCAA TCTGCGAAGC ACCCATCCTC ATTTTGTACG CTGCATCATC 
CCAAATGAAA CAAAAACACC TGGTGCCATG GAGCATGAAC TGGTGCTTCA CCAGCTGCGG 
TGTAACGGCG TGCTGGAAGG CATCAGAATT TGCAGGAAAG GATTTCCCAA CAGAGTCCTG 
TATGCAGATT TTAAACAGAG ATACAAAGTA TTAAATGCAA GTGCTATCCC GGAGGGACAG 
TTCATTGACA GCAAGAAGGC TTGTGAGAAA CTTCTTGGAT CAATTGATAT AGACCACACT 
CAATACAAAT TTGGTCACAC CAAGGTGTTC TTCAAAGCTG GGCTGATAGG CCTCTTGGAA 
GAGATGAGAG ATGAAAAGTT GGCACAGATC ATCACTCGCA CACAGGCAAG GTGCAGGGGC 
TTCCTGATGA GAGTGGAGTA CCAGAGAATG GTGGAGAGGA GGGAGTCCAT CTTCTGCATC 
CAGTACAACA TTCGGGCATT CATGAATGTC AAGCACTGGC CCTGGATGAA GCTGTTCTTC 
AAGATCAAGC CCTTGCTGAA GAGCGCCGAA TCTGAGAAGG AGATGGCCAA CATGAAACAA 
GAGTTTGAGA AAACCAAGGA AGAGCTTGCA AAGTCTGAGG CAAAGAGGAA GGAGCTGGAG 
GAGAAAATGG TGAAACTGGT GCAGGAAAAA AATGACCTGC AGCTCCAAGT GCAGGCTGAA 
GCTGATGCTT TGGCTGATGC TGAGGAAAGG TGCGACCAGC TCATTAAAAC CAAAATCCAG 
CTGGAAGCCA AAGTTAAAGA AGTGACTGAA AGGGCAGAGG ATGAGGAAGA AATTAACGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAGCTGAA GAAAGACATT 
GACGATCTTG AGCTAACACT GGCCAAGGTT GAGAAGGAAA AGCATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAGGA GATGGCAGCC CTGGATGAGA CCATTGTGAA GCTGACAAAA 
GAGAAGAAAG CCCTCCAAGA GGCCCATCAG CAGACACTGG ATGACCTGCA GGCAGAAGAG 
GACAAAGTCA ATACGCTGAC CAAAGCTAAG ACCAAGCTGG AGCAGCAAGT GGACGATCTG 
GAAGGGTCCC TGGAGCAGGA GAAGAAGCTG CGCATGGACC TTGAGAGAGC TAAGAGGAAA 
CTCGAAGGAG ACCTGAAGCT GGCCCATGAC AGCATAATGG ATTTGGAAAA TGATAAGCAG 
CAGCTGGATG AGAAACTGAA GAAGAAAGAC TTTGAAATCA GCCAGATCCA GAGCAAGATC 
GAGGATGAAC AACTTCTGGG CATCCAGTTA CAGAAGAAGA TCAAGGAGCT GCAGGCGCGT 
ATTGAGGAAC TGGAGGAGGA AATTGAGGCA GAGCGAACCT CTCGGGCAAA AGCAGAGAAG 
CATCGTGCTG ACCTCTCGAG GGAGCTGGAG GAGATCAGCG AGCGCCTGGA AGAAGCAGGA 
GGGGCCACTG CAGCTCAGAT TGAGATGAAC AAGAAGCGTG AGGCAGAATT TCAGAAGATG 
CGTCGCGACC TCGAAGAGGC CACGCTGCAG CACGAAGCCA CGGCTGCCGC CCTGCGGAAG 
AAGCACGCGG ACAGCACGGC CGAGCTCGGG GAGCAGATCG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGCTG AAGATGGAGA TTGATGACTT GGCCAGTAAC 
ATGGAGTCTG TCTCCAAAGC CAAGGCAAAC TTGGAGAAGA TGTGCCGCAC TCTGGAAGAC 
CAATTAAATG AATTTAAAAC AAAGGAGGAG CAGAATCAGC GCATGATCAG CGATCTCAGT 
GCTCAAAGAG CTCGTCTGCA GACAGAATCT GGGGAATATG CACGCCAGGC TGAGGAAAAA 
GATGGTTTAA TTTCTCAGCT GTCTAGAGGG AAACAGGCTT TCACCCAGCA GATTGAAGAA 
TTGAAGAGGC AACTAGAGGA AGAGATAAAG GCCAAGAATG CCCTGGCCCA CAGCTTGCAG 
TCTGCTCGCC ACGACTGTGA CTTGCTCCGG GAACAATATG AGGAGGAGCA GGAGGCCAAG 
GGGGAGCTGC AGCGTGCCCT GTCCAAGGCC AACAGCGAAG TGGCCCAGTG GAGAACCAAA 
TACGAGACGG ACGCTATTCA GCGCACGGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA 
CAGCGCCTGC AGGATGCAGA GGAACACGTT GAAGCTGTCA ATGCCAAATG TGCCTCCCTG 
GAAAAGACAA AGCAGAGGCT GCAGAATGAA GTGGAGGACC TGATGATTGA CGTGGAGCGA 
TCAAATGCTG CCTGCGCAGC TCTGGATAAG AAGCAGAAGA ACTTTGACAA GATCCTGGCA 
GAGTGGAAGC AGAAGTATGA GGAAACGCAG GCTGAGCTGG AAGCCTCCCA GAAGGAGTCT 
CGCTCTCTCA GCACGGAGCT CTTTAAGATG AAGAATGCCT ATGAGGAGTC CTTGGACCAC 
CTGGAAACGC TGAAGCGTGA GAACAAGAAC TTGCAGCAGG AGATTTCCGA CCTCACGGAG 
CAGATTGCCG AGGGAGGAAA GGCGATTCAT GAGCTGGAGA AAGTCAAGAA GCAGATTGAG 
CAGGAGAAAT CTGAACTCCA AGCCTCCCTG GAGGAAGCCG AGGCTTCTCT AGAACACGAA 
GAGGGGAAGA TCCTGCGCCT CCAGCTTGAG CTCAACCAGG TGAAGTCTGA GATTGACAGG 
AAGGTAGCAG AGAAAGATGA GGAGATCGAC CAGCTGAAGA GAAACCACCT CAGAATCGTG 
GAGTCCATGC AGAGCACCCT GGACGCTGAG ATCAGGAGCA GGAATGAAGC CCTGCGGCTG 
AAGAAGAAGA TGGAGGGAGA CCTGAATGAA ATGGAGATCC AGCTGAGCCA CGCCAACCGC 
CAGGCTGCAG AGGCACAAAA GAACCTGAGG AACACACAGG GAGTGCTCAA GGATACCCAG 
ATACACTTGG ACGACGCTCT CAGGACACAG GAGGACCTGA AGGAGCAGGT GGCCATGGTG 
GAGCGCAGAG CAAACCTGCT GCAGGCTGAA GTTGAGGAGC TGCGGGCAGC CCTGGAGCAG 
ACGGAGCGCT CGAGGAAAGT GGCTGAGCAG GAGCTTCTGG ATGCCACTGA ACGTGTGCAG 
CTCCTCCACA CCCAGAACAC CAGCTTAATT AACACCAAGA AGAAGCTGGA AACAGACATC 
ATGCAAATTC AGGGTGAAAT GGAGGATACG ATCCAGGAAG CGCGCAATGC TGAAGAGAAG 
GCCAAGAAGG CCATCACAGA TGCAGCCATG ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAAAG GATGAAGAAG AACCTGGACC AGACGGTGAA GGACCTGCAG 
CACCGTCTGG ATGAGGCGGA GCAGCTGGCT CTGAAGGGAG GCAAGAAGCA GCTCCAGAAG 
CTGGAGGCCA GAGTGCGGGA GCTGGAAGGG GAGGTGGATG CTGAGCAGAA GCGCAGCGCT 
GAAGCCGTGA AGGGTGTGCG CAAGTACGAG AGGAGGGTGA AGGAGCTGAC CTACCAGTCT 
GAGGAAGACC GGAAGAATAT TCTGAGGCTC CAGGATCTGG TGGACAAGCT GCAAACGAAG 
GTGAAATCCT ACAAGAGACA AGCTGAGGAG GCTGAGGAGC TCTCCAACGT CAACCTCTCC 
AAGTTCCGCA AGATCCAGCA CGAGCTGGAG GAAGCCGAGG AGCGGGCTGA CATTGCAGAG 
TCGCAGGTCA ACAAGCTGCG AGCGAAGAGT CGGGAGTTTC ATAGGAAGAT AGAAGAGGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106900570 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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