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EDN3 cDNA ORF clone, Canis lupus familiaris(Dog)

Summary

Gene Symbol EDN3
Entrez Gene ID 403406
Full Name endothelin 3
Synonyms PPET3
General protein information
Preferred Names

endothelin 3

Names

endothelin-3
ET-3
preproendothelin-3
Gene Type protein-coding
Organism Canis lupus familiaris(Dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005634662.3 XP_005634719.1 endothelin-3 isoform X1
XM_022408801.1 XP_022264509.1 endothelin-3 isoform X3
NM_001002942.1 NP_001002942.1 endothelin-3 precursor
XM_022408799.1 XP_022264507.1 endothelin-3 isoform X2
XM_022408800.1 XP_022264508.1 endothelin-3 isoform X3
XM_005634662.2 XP_005634719.1 endothelin-3 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) EDN3 NP_996915.1
Pan troglodytes (chimpanzee) EDN3 XP_525373.2
Bos taurus (cattle) EDN3 NP_001095449.1
Mus musculus (house mouse) Edn3 NP_031929.1
Rattus norvegicus (Norway rat) Edn3 NP_001071118.1
Canis lupus familiaris (dog) EDN3 NP_001002942.1
Gallus gallus (chicken) EDN3 XP_001231488.3

Gene Ontology


Bibliography

Related articles in PubMed

Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.
Fujimori Y, Uchide T, Temma K, Sasaki T, Kizaki K, Hara Y, Takizawa S, Saida K
Journal of cardiovascular pharmacology44 Suppl 1S426-9(2004 Nov)

Complete cDNA sequence and mRNA expression of dog preproendothelin-3.
Fujimori Y, Uchide T, Temma K, Sasaki T, Kizaki K, Hara Y, Saida K
The Journal of veterinary medical science66(10)1251-4(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.
Title: Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.

The following EDN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe33605 NM_001002942.1
Latest version!		 Canis lupus familiaris endothelin 3 (EDN3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe24420 XM_005634662.3
Latest version!		 Canis lupus familiaris endothelin 3 (EDN3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OCe296587 XM_022408799.1
Latest version!		 Canis lupus familiaris endothelin 3 (EDN3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OCe296588 XM_022408800.1
Latest version!		 Canis lupus familiaris endothelin 3 (EDN3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OCe296588 XM_022408801.1
Latest version!		 Canis lupus familiaris endothelin 3 (EDN3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
View Old Accession Versions >>
OCe24420 XM_005634662.2 Canis lupus familiaris endothelin 3 (EDN3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe33605
Clone ID Related Accession (Same CDS sequence) NM_001002942.1
Accession Version NM_001002942.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Canis lupus familiaris(dog)
Product endothelin-3 precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB115086.1. ##Evidence-Data-START## Transcript exon combination :: AB115086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA2240594 [ECO:0000348] ##Evidence-Data-END## 

1
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ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGCA AGAACTAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGCAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001002942.1
CDS75..671
Translation

Target ORF information:

RefSeq Version NM_001002942.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001002942.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGCA AGAACTAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGCAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe24420
Clone ID Related Accession (Same CDS sequence) XM_005634662.3 , XM_005634662.2
Accession Version XM_005634662.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product endothelin-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
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421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTTGAAG 
CCAAGGACAG ATGGAGGGAG CAGGCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005634719.1
CDS191..820
Translation

Target ORF information:

RefSeq Version XM_005634662.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005634662.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTTGAAG 
CCAAGGACAG ATGGAGGGAG CAGGCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe24420
Clone ID Related Accession (Same CDS sequence) XM_005634662.3 , XM_005634662.2
Accession Version XM_005634662.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product endothelin-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
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421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTTGAAG 
CCAAGGACAG ATGGAGGGAG CAGGCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005634719.1
CDS234..863
Translation

Target ORF information:

RefSeq Version XM_005634662.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005634662.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGGTTGAAG 
CCAAGGACAG ATGGAGGGAG CAGGCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe296587
Clone ID Related Accession (Same CDS sequence) XM_022408799.1
Accession Version XM_022408799.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 600bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product endothelin-3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
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481
541
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022264507.1
CDS190..789
Translation

Target ORF information:

RefSeq Version XM_022408799.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022408799.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTCTTCGGG CTCACGGTGA CCTCCGCCGC AGGATTGGTA 
CCTTGCCCCC AGCCTGGGGA CGCTGGCAAA AGCGGCGTGC CCGGGACCCC CCCTACAGCC 
AGATCTGAGG GGGACATCCA GGAGCCTGTG GCCATGACTG CAGTGCAAGG TCCAAGTCCC 
AGAAGCCCCG AGCAGGAGAA AGAACCAGGT CGGTTTGGGG AGCAGGCATC CAAGGGGGGT 
CCTGTGCACG GCCGCGCCAG GCGCTGTACC TGCTTCACAT ACAAGGACAA GGAGTGTGTC 
TACTATTGCC ACCTGGACAT CATCTGGATC AACACTCCTG AACAGACTGT GCCCTATGGA 
CTGTCCAACT ACAGGGGGAG CTTCCGCGGC AGGAGGTCCA CGGGGCTGTT CCCGCAGAGC 
CCACAGCCCT CCAAGTGGAC ACAGCGCTGC GCTTGTGTGC AGAGCCAGGA CAGCGCCTGC 
TTGCACTTCT GCACCCGCAC CCTGGCTGTC AGGAGGAATT CAAGGACAGC AACAAATCCT 
GACAAAGAGG AAGAGCCAGC CAGCCGTGGC AACGGGGGGC TGCGTCCAAC GAGCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe296588
Clone ID Related Accession (Same CDS sequence) XM_022408801.1 , XM_022408800.1
Accession Version XM_022408801.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product endothelin-3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
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481
541
ATGTCAGGAT TGGTACCTTG CCCCCAGCCT GGGGACGCTG GCAAAAGCGG CGTGCCCGGG 
ACCCCCCCTA CAGCCAGATC TGAGGGGGAC ATCCAGGAGC CTGTGGCCAT GACTGCAGTG 
CAAGGTCCAA GTCCCAGAAG CCCCGAGCAG GAGAAAGAAC CAGGTCGGTT TGGGGAGCAG 
GCATCCAAGG GGGGTCCTGT GCACGGCCGC GCCAGGCGCT GTACCTGCTT CACATACAAG 
GACAAGGAGT GTGTCTACTA TTGCCACCTG GACATCATCT GGATCAACAC TCCTGAACAG 
ACTGTGCCCT ATGGACTGTC CAACTACAGG GGGAGCTTCC GCGGCAGGAG GTCCACGGGG 
CTGTTCCCGC AGAGCCCACA GCCCTCCAAG TGGACACAGC GCTGCGCTTG TGTGCAGAGC 
CAGGACAGCG CCTGCTTGCA CTTCTGCACC CGCACCCTGG CTGTCAGGAG GAATTCAAGG 
ACAGCAACAA ATCCTGACAA AGAGGAAGAG CCAGCCAGCC GTGGCAACGG GGGGCTGCGT 
CCAACGAGGT TGAAGCCAAG GACAGATGGA GGGAGCAGGC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022264509.1
CDS193..777
Translation

Target ORF information:

RefSeq Version XM_022408801.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022408801.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCAGGAT TGGTACCTTG CCCCCAGCCT GGGGACGCTG GCAAAAGCGG CGTGCCCGGG 
ACCCCCCCTA CAGCCAGATC TGAGGGGGAC ATCCAGGAGC CTGTGGCCAT GACTGCAGTG 
CAAGGTCCAA GTCCCAGAAG CCCCGAGCAG GAGAAAGAAC CAGGTCGGTT TGGGGAGCAG 
GCATCCAAGG GGGGTCCTGT GCACGGCCGC GCCAGGCGCT GTACCTGCTT CACATACAAG 
GACAAGGAGT GTGTCTACTA TTGCCACCTG GACATCATCT GGATCAACAC TCCTGAACAG 
ACTGTGCCCT ATGGACTGTC CAACTACAGG GGGAGCTTCC GCGGCAGGAG GTCCACGGGG 
CTGTTCCCGC AGAGCCCACA GCCCTCCAAG TGGACACAGC GCTGCGCTTG TGTGCAGAGC 
CAGGACAGCG CCTGCTTGCA CTTCTGCACC CGCACCCTGG CTGTCAGGAG GAATTCAAGG 
ACAGCAACAA ATCCTGACAA AGAGGAAGAG CCAGCCAGCC GTGGCAACGG GGGGCTGCGT 
CCAACGAGGT TGAAGCCAAG GACAGATGGA GGGAGCAGGC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe296588
Clone ID Related Accession (Same CDS sequence) XM_022408801.1 , XM_022408800.1
Accession Version XM_022408800.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product endothelin-3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGTCAGGAT TGGTACCTTG CCCCCAGCCT GGGGACGCTG GCAAAAGCGG CGTGCCCGGG 
ACCCCCCCTA CAGCCAGATC TGAGGGGGAC ATCCAGGAGC CTGTGGCCAT GACTGCAGTG 
CAAGGTCCAA GTCCCAGAAG CCCCGAGCAG GAGAAAGAAC CAGGTCGGTT TGGGGAGCAG 
GCATCCAAGG GGGGTCCTGT GCACGGCCGC GCCAGGCGCT GTACCTGCTT CACATACAAG 
GACAAGGAGT GTGTCTACTA TTGCCACCTG GACATCATCT GGATCAACAC TCCTGAACAG 
ACTGTGCCCT ATGGACTGTC CAACTACAGG GGGAGCTTCC GCGGCAGGAG GTCCACGGGG 
CTGTTCCCGC AGAGCCCACA GCCCTCCAAG TGGACACAGC GCTGCGCTTG TGTGCAGAGC 
CAGGACAGCG CCTGCTTGCA CTTCTGCACC CGCACCCTGG CTGTCAGGAG GAATTCAAGG 
ACAGCAACAA ATCCTGACAA AGAGGAAGAG CCAGCCAGCC GTGGCAACGG GGGGCTGCGT 
CCAACGAGGT TGAAGCCAAG GACAGATGGA GGGAGCAGGC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022264508.1
CDS274..858
Translation

Target ORF information:

RefSeq Version XM_022408800.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris endothelin 3 (EDN3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022408800.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCAGGAT TGGTACCTTG CCCCCAGCCT GGGGACGCTG GCAAAAGCGG CGTGCCCGGG 
ACCCCCCCTA CAGCCAGATC TGAGGGGGAC ATCCAGGAGC CTGTGGCCAT GACTGCAGTG 
CAAGGTCCAA GTCCCAGAAG CCCCGAGCAG GAGAAAGAAC CAGGTCGGTT TGGGGAGCAG 
GCATCCAAGG GGGGTCCTGT GCACGGCCGC GCCAGGCGCT GTACCTGCTT CACATACAAG 
GACAAGGAGT GTGTCTACTA TTGCCACCTG GACATCATCT GGATCAACAC TCCTGAACAG 
ACTGTGCCCT ATGGACTGTC CAACTACAGG GGGAGCTTCC GCGGCAGGAG GTCCACGGGG 
CTGTTCCCGC AGAGCCCACA GCCCTCCAAG TGGACACAGC GCTGCGCTTG TGTGCAGAGC 
CAGGACAGCG CCTGCTTGCA CTTCTGCACC CGCACCCTGG CTGTCAGGAG GAATTCAAGG 
ACAGCAACAA ATCCTGACAA AGAGGAAGAG CCAGCCAGCC GTGGCAACGG GGGGCTGCGT 
CCAACGAGGT TGAAGCCAAG GACAGATGGA GGGAGCAGGC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.
Journal of cardiovascular pharmacology44 Suppl 1S426-9(2004 Nov)
Fujimori Y,Uchide T,Temma K,Sasaki T,Kizaki K,Hara Y,Takizawa S,Saida K


book

Complete cDNA sequence and mRNA expression of dog preproendothelin-3.
The Journal of veterinary medical science66(10)1251-4(2004 Oct)
Fujimori Y,Uchide T,Temma K,Sasaki T,Kizaki K,Hara Y,Saida K