ORF cDNA Clones and Custom Clones » Species Summary » Canis lupus familiaris » CHRNA1 cDNA ORF clone

CHRNA1 cDNA ORF clone, Canis lupus familiaris(dog)

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Summary

Gene Symbol	CHRNA1
Entrez Gene ID	403757
Full Name	cholinergic receptor nicotinic alpha 1 subunit
General protein information	Preferred Names cholinergic receptor nicotinic alpha 1 subunit Names acetylcholine receptor subunit alpha cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) muscle nicotinic acetylcholine receptor alpha-su exon P4 nachr alpha-subunit nicotinic acetylcholine recepter alpha-subunit nicotinic alpha polypeptide 1 nicotinic cholinergic receptor alpha polypeptide 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003144.2	NP_001003144.1	acetylcholine receptor subunit alpha precursor

Homologies

Danio rerio (zebrafish)	chrna1	NP_571520.1
Gallus gallus (chicken)	CHRNA1	NP_990147.1
Pan troglodytes (chimpanzee)	CHRNA1	XP_003309365.1
Mus musculus (house mouse)	Chrna1	NP_031415.2
Homo sapiens (human)	CHRNA1	NP_001034612.1
Canis lupus familiaris (dog)	CHRNA1	NP_001003144.1
	CHRNA1	XP_001091711.1
Bos taurus (cattle)	CHRNA1	NP_788837.1
Rattus norvegicus (Norway rat)	Chrna1	NP_077811.1
Xenopus tropicalis (tropical clawed frog)	chrna1	XP_002934647.1

Bibliography

Related articles in PubMed

Molecular cloning of the canine nicotinic acetylcholine receptor alpha-subunit gene and development of the ELISA method to diagnose myasthenia gravis.
Yoshioka T, Uzuka Y, Tanabe S, Sarashina T, Ishiguro N
Veterinary immunology and immunopathology72(3-4)315-24(1999 Dec)

Human nicotinic acetylcholine receptor alpha-subunit isoforms: origins and expression.
MacLennan C, Beeson D, Vincent A, Newsom-Davis J
Nucleic acids research21(23)5463-7(1993 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following CHRNA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33856	NM_001003144.2 Latest version!	Canis lupus familiaris cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA1 ( NM_001003144.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003144.1 Canis lupus familiaris cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), mRNA.

Price & Availability↑

CloneID	OCe33856
Clone ID Related Accession (Same CDS sequence)	NM_001003144.2
Accession Version	NM_001003144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Canis lupus familiaris(dog)
Product	acetylcholine receptor subunit alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB021708.1. On Aug 30, 2012 this sequence version replaced NM_001003144.1. ##Evidence-Data-START## Transcript exon combination :: AB021708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547742, SAMN00013515 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCT GGCCTCTCCT CCTGCTGCTT GGGCTCTGCT CAGCTGGCGT CGTCGTGGGC 
TCTGAACATG AGACCCGCCT GGTGGCAAAG CTATTTGAAG ATTACAACAG TGTGGTTCGG 
CCAGTGGAAG ACCACCGCCA GGCCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAACGTGG ATGAAGTCAA TCAGATTGTG ACAACCAATG TGCGGCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAG GACTATGGTG GCGTGAAAAA AATTCACATT 
CCCTCGGAAA AGATCTGGCG CCCAGACCTC GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCCATTGTCA AGTTCACCAA AGTGCTCCTG GACTACACGG GCCACATCAC ATGGACACCT 
CCGGCCATCT TCAAAAGCTA CTGTGAGATC ATTGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TATGACGGCT CGGTGGTGGC CATTAACCCG 
GAAAGCGACC AGCCCGACCT GAGCAACTTC ATGGAAAGTG GAGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTG GGTGTTTTAT GCCTGCTGCC CCTCCACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCTCTCTACT TCATCGTCAA CGTCATCATT 
CCCTGCCTGC TCTTCTCCTT CTTAACCGGC CTGGTGTTCT ACCTCCCTAC GGACTCAGGA 
GAGAAGATGA CTCTAAGCAT CTCTGTCCTG CTGTCTCTAA CCGTGTTCCT CCTGGTCATC 
GTGGAGCTGA TCCCTTCCAC CTCCAGCGCC GTGCCCTTGA TTGGGAAGTA CATGCTGTTC 
ACCATGGTGT TTGTCATCGC GTCCATCATC ATCACCGTCA TCGTCATCAA CACACACCAC 
CGCTCCCCCA GCACCCACGT CATGCCCGAC TGGGTACGCA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTCTTCTC CACAATGAAA AGACCATCCA GAGAAAAACA AGACAAAAAG 
ATTTTTACGG AAGACATTGA CATTTCTGAC ATTTCTGGGA AGCCAGGGCC TCCACCCATG 
GGTTTCCACT CTCCCCTGAT CAAACATCCC GAGGTAAAAA GTGCCATTGA AGGAGTCAAA 
TACATCGCAG AGACGATGAA GTCAGACCAG GAGTCCAATA ATGCGGCTGA GGAATGGAAG 
TATGTTGCAA TGGTGATGGA CCACATCCTC CTTGGAGTCT TCATGCTCGT CTGTATCATT 
GGAACCCTGG CTGTGTTTGC AGGTCGACTC ATTGAATTAA ATCTGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003144.1
CDS	5..1378
Translation	MEPWPLLLLLGLCSAGVVVGSEHETRLVAKLFEDYNSVVRPVEDHRQAVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPEDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLDYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHWVFYACCPSTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPDWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGVKYIAETMKSDQESNNAAEEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNLQG

Target ORF information:

RefSeq Version	NM_001003144.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003144.2

ORF Insert Sequence:

ATGGAGCCCT GGCCTCTCCT CCTGCTGCTT GGGCTCTGCT CAGCTGGCGT CGTCGTGGGC 
TCTGAACATG AGACCCGCCT GGTGGCAAAG CTATTTGAAG ATTACAACAG TGTGGTTCGG 
CCAGTGGAAG ACCACCGCCA GGCCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAACGTGG ATGAAGTCAA TCAGATTGTG ACAACCAATG TGCGGCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAG GACTATGGTG GCGTGAAAAA AATTCACATT 
CCCTCGGAAA AGATCTGGCG CCCAGACCTC GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCCATTGTCA AGTTCACCAA AGTGCTCCTG GACTACACGG GCCACATCAC ATGGACACCT 
CCGGCCATCT TCAAAAGCTA CTGTGAGATC ATTGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TATGACGGCT CGGTGGTGGC CATTAACCCG 
GAAAGCGACC AGCCCGACCT GAGCAACTTC ATGGAAAGTG GAGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTG GGTGTTTTAT GCCTGCTGCC CCTCCACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCTCTCTACT TCATCGTCAA CGTCATCATT 
CCCTGCCTGC TCTTCTCCTT CTTAACCGGC CTGGTGTTCT ACCTCCCTAC GGACTCAGGA 
GAGAAGATGA CTCTAAGCAT CTCTGTCCTG CTGTCTCTAA CCGTGTTCCT CCTGGTCATC 
GTGGAGCTGA TCCCTTCCAC CTCCAGCGCC GTGCCCTTGA TTGGGAAGTA CATGCTGTTC 
ACCATGGTGT TTGTCATCGC GTCCATCATC ATCACCGTCA TCGTCATCAA CACACACCAC 
CGCTCCCCCA GCACCCACGT CATGCCCGAC TGGGTACGCA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTCTTCTC CACAATGAAA AGACCATCCA GAGAAAAACA AGACAAAAAG 
ATTTTTACGG AAGACATTGA CATTTCTGAC ATTTCTGGGA AGCCAGGGCC TCCACCCATG 
GGTTTCCACT CTCCCCTGAT CAAACATCCC GAGGTAAAAA GTGCCATTGA AGGAGTCAAA 
TACATCGCAG AGACGATGAA GTCAGACCAG GAGTCCAATA ATGCGGCTGA GGAATGGAAG 
TATGTTGCAA TGGTGATGGA CCACATCCTC CTTGGAGTCT TCATGCTCGT CTGTATCATT 
GGAACCCTGG CTGTGTTTGC AGGTCGACTC ATTGAATTAA ATCTGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular cloning of the canine nicotinic acetylcholine receptor alpha-subunit gene and development of the ELISA method to diagnose myasthenia gravis.
Veterinary immunology and immunopathology72(3-4)315-24(1999 Dec)
Yoshioka T,Uzuka Y,Tanabe S,Sarashina T,Ishiguro N

Human nicotinic acetylcholine receptor alpha-subunit isoforms: origins and expression.
Nucleic acids research21(23)5463-7(1993 Nov)
MacLennan C,Beeson D,Vincent A,Newsom-Davis J

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CHRNA1 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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