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S100A4 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol S100A4
Entrez Gene ID 403787
Full Name S100 calcium binding protein A4
Synonyms MTS1
General protein information
Preferred Names

S100 calcium binding protein A4

Names

protein S100-A4
S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
metastasin
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001003161.2 NP_001003161.1 protein S100-A4 isoform 1
NM_001003161.3 NP_001003161.1 protein S100-A4 isoform 1
XM_005622226.2 XP_005622283.1 protein S100-A4 isoform X1
NM_001362597.2 NP_001349526.2 protein S100-A4 isoform 2
NM_001362597.3 NP_001349526.2 protein S100-A4 isoform 2
XM_022420732.1 XP_022276440.1 protein S100-A4 isoform X2
NM_001003161.1 NP_001003161.1 protein S100-A4
XM_005622226.3 XP_005622283.2 protein S100-A4 isoform X1
NM_001363554.1 NP_001350483.1 protein S100-A4 isoform 1

Pathways from BioSystems


Homologies


Homo sapiens (human) S100A4 NP_002952.1
S100A4 XP_001110673.1
Danio rerio (zebrafish) icn NP_997926.1
Danio rerio (zebrafish) icn2 NP_001076426.1
Mus musculus (house mouse) S100a4 NP_035441.1
Gallus gallus (chicken) S100A4 XP_004948276.1
Canis lupus familiaris (dog) S100A4 NP_001003161.1
Bos taurus (cattle) S100A4 NP_777020.1
Rattus norvegicus (Norway rat) S100a4 NP_036750.1
Pan troglodytes (chimpanzee) S100A4 XP_001138744.1

Gene Ontology

The following S100A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe08925 NM_001363554.1
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant b, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe08925 NM_001003161.3
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant a, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe08925 XM_005622226.2
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe08925 XM_022420732.1
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe289379 NM_001362597.3
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant c, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OCe289379 XM_005622226.3
Latest version!		 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
View Old Accession Versions >>
OCe08925 NM_001003161.2 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant a, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OCe289379 NM_001362597.2 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant c, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OCe08925 NM_001003161.1 Canis lupus familiaris S100 calcium binding protein A4 (S100A4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version NM_001003161.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-12-22
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005359.1. On Apr 20, 2018 this sequence version replaced NM_001003161.1. Transcript Variant: This variant (a, also known as variant 1) encodes isoform 1. Variants a and b both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN873058.1, DN873656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003161.1
CDS57..362
Translation

Target ORF information:

RefSeq Version NM_001003161.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant a, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003161.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version NM_001003161.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-27
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005359.1. On Feb 28, 2019 this sequence version replaced NM_001003161.2. Transcript Variant: This variant (a, also known as variant 1) encodes isoform 1. Variants a and b both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN873656.1, DN870609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003161.1
CDS57..362
Translation

Target ORF information:

RefSeq Version NM_001003161.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant a, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003161.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version NM_001003161.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-21
Organism Canis lupus familiaris(dog)
Product protein S100-A4
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB031064.1. On Jan 12, 2005 this sequence version replaced XM_537266.1. ##Evidence-Data-START## Transcript exon combination :: AB031064.1, DN866180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003161.1
CDS51..356
Translation

Target ORF information:

RefSeq Version NM_001003161.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003161.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version NM_001363554.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-26
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005359.1. On May 24, 2018 this sequence version replaced XM_022420732.1. Transcript Variant: This variant (b, also known as variant 4) differs in the 5' UTR compared to variant a, and encodes the same protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: AB031064.1, CO674592.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350483.1
CDS132..437
Translation

Target ORF information:

RefSeq Version NM_001363554.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant b, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363554.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version XM_005622226.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product protein S100-A4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005622283.1
CDS115..420
Translation

Target ORF information:

RefSeq Version XM_005622226.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005622226.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08925
Clone ID Related Accession (Same CDS sequence) NM_001003161.2 , NM_001003161.3 , XM_005622226.2 , XM_022420732.1 , NM_001003161.1 , NM_001363554.1
Accession Version XM_022420732.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022276440.1
CDS132..437
Translation

Target ORF information:

RefSeq Version XM_022420732.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022420732.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGACGTTCC CCCTGGAGAA GGCTCTGGAT GTGATGGTGT CTACTTTCCA CAAGTACTCT 
GGCAAGGAGG GTGACAAGTT CAAGCTCAAC AGATCAGAGT TAAAGGAGCT GCTGATGCGG 
GAGCTGCCCA GCTTCTTGGG GAAAAGGACG GATGAAGCCG CCTTCCAAAA GCTGATGAGC 
AACTTGGACA GCAACAGAGA CAACGAGGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCGTCGCCA TGATGTGCAA TGAATTCTTC GAAGGCTTCC CTGATAAGCA GCCCCGGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe289379
Clone ID Related Accession (Same CDS sequence) NM_001362597.2 , NM_001362597.3 , XM_005622226.3
Accession Version NM_001362597.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-07
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from MH294432.1. On or before Sep 7, 2018 this sequence version replaced NM_001363553.1, NM_001362597.1. Transcript Variant: This variant (c, also known as variant 3) differs in the 5' UTR and 5' coding region compared to variant a. The encoded isoform (2) has a longer N-terminus than isoform 1. COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001349526.2
CDS33..461
Translation

Target ORF information:

RefSeq Version NM_001362597.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant c, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001362597.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe289379
Clone ID Related Accession (Same CDS sequence) NM_001362597.2 , NM_001362597.3 , XM_005622226.3
Accession Version NM_001362597.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-27
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005359.1. On or before Feb 28, 2019 this sequence version replaced XM_005622226.3, NM_001362597.2. Transcript Variant: This variant (c, also known as variant 3) differs in the 5' UTR and 5' coding region compared to variant a. The encoded isoform (2) has a longer N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN870915.1, DN870914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001349526.2
CDS33..461
Translation

Target ORF information:

RefSeq Version NM_001362597.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant c, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001362597.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe289379
Clone ID Related Accession (Same CDS sequence) NM_001362597.2 , NM_001362597.3 , XM_005622226.3
Accession Version XM_005622226.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product protein S100-A4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005622283.2
CDS44..472
Translation

Target ORF information:

RefSeq Version XM_005622226.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris S100 calcium binding protein A4 (S100A4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005622226.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGGGCAGG GTGGGGCTGC TGTGGTCGCC GGGAGTACTG GGGGGGCAGT CGGTGAGGCC 
CAGCCTGAGG GATGCTGCCT GACCCACACA GCCTGTGCAA CGGTCCTGGT CCTGACAGCC 
ACCATGACGT TCCCCCTGGA GAAGGCTCTG GATGTGATGG TGTCTACTTT CCACAAGTAC 
TCTGGCAAGG AGGGTGACAA GTTCAAGCTC AACAGATCAG AGTTAAAGGA GCTGCTGATG 
CGGGAGCTGC CCAGCTTCTT GGGGAAAAGG ACGGATGAAG CCGCCTTCCA AAAGCTGATG 
AGCAACTTGG ACAGCAACAG AGACAACGAG GTTGACTTCC AGGAGTACTG TGTCTTCCTG 
TCCTGCGTCG CCATGATGTG CAATGAATTC TTCGAAGGCT TCCCTGATAA GCAGCCCCGG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed