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NEFH cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol NEFH
Entrez Gene ID 442940
Full Name neurofilament heavy
Synonyms NFH
General protein information
Preferred Names

neurofilament heavy

Names

neurofilament heavy polypeptide
heavy neurofilament protein
intermediate filament protein
neurofilament, heavy polypeptide 200kDa
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005636010.3 XP_005636067.1 neurofilament heavy polypeptide isoform X5
XM_005636006.2 XP_005636063.1 neurofilament heavy polypeptide isoform X3
XM_005636010.2 XP_005636067.1 neurofilament heavy polypeptide isoform X8
XM_005636012.2 XP_005636069.1 neurofilament heavy polypeptide isoform X9
XM_005636008.2 XP_005636065.1 neurofilament heavy polypeptide isoform X5
NM_001003352.1 NP_001003352.1 neurofilament heavy polypeptide
XM_014107712.2 XP_013963187.1 neurofilament heavy polypeptide isoform X2
XM_005636004.2 XP_005636061.1 neurofilament heavy polypeptide isoform X2
XM_005636008.3 XP_005636065.1 neurofilament heavy polypeptide isoform X3
XM_014107712.1 XP_013963187.1 neurofilament heavy polypeptide isoform X4
XM_005636009.2 XP_005636066.1 neurofilament heavy polypeptide isoform X6
XM_005636011.2 XP_005636068.1 neurofilament heavy polypeptide isoform X7
XM_005636006.3 XP_005636063.1 neurofilament heavy polypeptide isoform X1
XM_005636003.2 XP_005636060.1 neurofilament heavy polypeptide isoform X1
XM_005636009.3 XP_005636066.1 neurofilament heavy polypeptide isoform X4

Pathways from BioSystems


Homologies


Homo sapiens (human) NEFH NP_066554.2
Mus musculus (house mouse) Nefh NP_035034.2
Rattus norvegicus (Norway rat) Nefh NP_036739.2
Pan troglodytes (chimpanzee) NEFH XP_515058.3
Canis lupus familiaris (dog) NEFH NP_001003352.1
Bos taurus (cattle) NEFH XP_005218170.1
Gallus gallus (chicken) NEFH XP_415310.4

Gene Ontology


Bibliography

Related articles in PubMed

Combination of serum phosphorylated neurofilament heavy subunit and hyperintensity of intramedullary T2W on magnetic resonance imaging provides better prognostic value of canine thoracolumbar intervertebral disc herniation.
Mashita T, Kamishina H, Nakamoto Y, Akagi Y, Nakanishi A, Harasaki Y, Ozawa T, Uemura T, Kobatake Y, Shimamura S, Kitamura N, Maeda S, Uzuka Y, Shaw G, Yasuda J
The Journal of veterinary medical science77(4)433-8(2015 Apr)

Evaluation of serum phosphorylated neurofilament subunit NF-H as a prognostic biomarker in dogs with thoracolumbar intervertebral disc herniation.
Nishida H, Nakayama M, Tanaka H, Kamishina H, Izawa T, Hatoya S, Sugiura K, Suzuki Y, Ide C, Inaba T
Veterinary surgery : VS43(3)289-93(2014 Mar)

Allelic variants of the canine heavy neurofilament (NFH) subunit and extensive phosphorylation in dogs with motor neuron disease.
Green SL, Westendorf JM, Jaffe H, Pant HC, Cork LC, Ostrander EA, Vignaux F, Ferrell JE Jr
Journal of comparative pathology132(1)33-50(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Combined measurements of serum pNF-H and T2H/L2 might be useful for predicting long-term outcome in dogs with thoracolumbar intervertebral disc herniation.
Title: Combination of serum phosphorylated neurofilament heavy subunit and hyperintensity of intramedullary T2W on magnetic resonance imaging provides better prognostic value of canine thoracolumbar intervertebral disc herniation.

The following NEFH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe33970 NM_001003352.1
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26007 XM_005636003.2
Latest version!		 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26008 XM_005636004.2
Latest version!		 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26010 XM_005636006.3
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26012 XM_005636008.3
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26013 XM_005636009.3
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe26014 XM_005636010.3
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe26015 XM_005636011.2
Latest version!		 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe26016 XM_005636012.2
Latest version!		 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe43184 XM_014107712.2
Latest version!		 Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe26014 XM_005636010.2 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe26012 XM_005636008.2 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe43184 XM_014107712.1 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26010 XM_005636006.2 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe26013 XM_005636009.2 Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe33970
Clone ID Related Accession (Same CDS sequence) NM_001003352.1
Accession Version NM_001003352.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-01-11
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF346625.1. ##Evidence-Data-START## Transcript exon combination :: AF346625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## 

1
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961
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1081
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1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGTCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCCGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGGGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GACCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCCGAGAA GGCCAAGTCT 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CTCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAAG CCAAATCCCC AGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAATCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCAGAGAA GCCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCTGAAAA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCTGAGAAAG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGAGAAGG TCAAATCTCC TGTGAAAGAA GAGACCAAGG CTCCTGAGAA AGAGGTCACA 
AAGAAGGAAG AGGCAAAGTC CCCCATAAAG GAGGAAGAGA AACCCCAGGA AGTGAAAGTC 
AAAGAGCCCG CAAAGAAGGC AGAGGAAGAG AAAGCTCCAG CCACACCAAA AACTGAGGAG 
AAGAAGGACA GCAAGAAAGA TGAGGTGCCA AAGAAGGAGG CTCCAAAGCC TGAGGTCCCC 
GAAAAGAAGG AGCCTGCTGT GGAGAAACCC AAAGAATCCA AAGTTGAAGC CAAGAAAGAG 
ACTGAAGATA AGAAAAAAGC AGTGACCCCA GAGAAGGAGG TTCCTGCCAA GGTGAAGGAA 
GAAGCCAAAC CCAAAGAGAA GGCTGAGGTG GCCAAGAAGG AGCAAGATGA TGCCAAGGCC 
AAAGAACCCA GCAAAGCAGC AGAGAAGGAG CCAGAAAAGC CAAAGAAGGA AGGGACACCT 
GCAGCACCCG AGAAAAAAGA TGTCAAGGAG GAGAAGACCC CAGAAGCCAA GAAATCTGAG 
GAGAAACCCA AAGCAGAGGC CCCAGCCAAA GAAGAGCCCA GCAAGGAGGC CCCCACACCT 
GGCAAAGCCA AGACAGAAAA GGCTGAGAAA TCCTCTAGCA CAGACCAAAA AGACAGCAGG 
CCTGCAGAGA AGGCCACAGA AGACAAGGCC TCCAAGGGGG AGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003352.1
CDS68..3475
Translation

Target ORF information:

RefSeq Version NM_001003352.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003352.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGTCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCCGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGGGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GACCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCCGAGAA GGCCAAGTCT 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CTCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAAG CCAAATCCCC AGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAATCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCAGAGAA GCCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCTGAAAA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCTGAGAAAG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGAGAAGG TCAAATCTCC TGTGAAAGAA GAGACCAAGG CTCCTGAGAA AGAGGTCACA 
AAGAAGGAAG AGGCAAAGTC CCCCATAAAG GAGGAAGAGA AACCCCAGGA AGTGAAAGTC 
AAAGAGCCCG CAAAGAAGGC AGAGGAAGAG AAAGCTCCAG CCACACCAAA AACTGAGGAG 
AAGAAGGACA GCAAGAAAGA TGAGGTGCCA AAGAAGGAGG CTCCAAAGCC TGAGGTCCCC 
GAAAAGAAGG AGCCTGCTGT GGAGAAACCC AAAGAATCCA AAGTTGAAGC CAAGAAAGAG 
ACTGAAGATA AGAAAAAAGC AGTGACCCCA GAGAAGGAGG TTCCTGCCAA GGTGAAGGAA 
GAAGCCAAAC CCAAAGAGAA GGCTGAGGTG GCCAAGAAGG AGCAAGATGA TGCCAAGGCC 
AAAGAACCCA GCAAAGCAGC AGAGAAGGAG CCAGAAAAGC CAAAGAAGGA AGGGACACCT 
GCAGCACCCG AGAAAAAAGA TGTCAAGGAG GAGAAGACCC CAGAAGCCAA GAAATCTGAG 
GAGAAACCCA AAGCAGAGGC CCCAGCCAAA GAAGAGCCCA GCAAGGAGGC CCCCACACCT 
GGCAAAGCCA AGACAGAAAA GGCTGAGAAA TCCTCTAGCA CAGACCAAAA AGACAGCAGG 
CCTGCAGAGA AGGCCACAGA AGACAAGGCC TCCAAGGGGG AGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26007
Clone ID Related Accession (Same CDS sequence) XM_005636003.2
Accession Version XM_005636003.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA AGCCAAATCC CCAGAGAAGG CCAAGTCTCC AGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAATCC CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGCCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AAAAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGTCAAATCT 
CCTGTGAAAG AAGAGACCAA GGCTCCTGAG AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG 
TCCCCCATAA AGGAGGAAGA GAAACCCCAG GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG 
GCAGAGGAAG AGAAAGCTCC AGCCACACCA AAAACTGAGG AGAAGAAGGA CAGCAAGAAA 
GATGAGGTGC CAAAGAAGGA GGCTCCAAAG CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT 
GTGGAGAAAC CCAAAGAATC CAAAGTTGAA GCCAAGAAAG AGACTGAAGA TAAGAAAAAA 
GCAGTGACCC CAGAGAAGGA GGTTCCTGCC AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG 
AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT GATGCCAAGG CCAAAGAACC CAGCAAAGCA 
GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG GAAGGGACAC CTGCAGCACC CGAGAAAAAA 
GATGTCAAGG AGGAGAAGAC CCCAGAAGCC AAGAAATCTG AGGAGAAACC CAAAGCAGAG 
GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG GCCCCCACAC CTGGCAAAGC CAAGACAGAA 
AAGGCTGAGA AATCCTCTAG CACAGACCAA AAAGACAGCA GGCCTGCAGA GAAGGCCACA 
GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636060.1
CDS76..3465
Translation

Target ORF information:

RefSeq Version XM_005636003.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636003.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA AGCCAAATCC CCAGAGAAGG CCAAGTCTCC AGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAATCC CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGCCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AAAAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGTCAAATCT 
CCTGTGAAAG AAGAGACCAA GGCTCCTGAG AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG 
TCCCCCATAA AGGAGGAAGA GAAACCCCAG GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG 
GCAGAGGAAG AGAAAGCTCC AGCCACACCA AAAACTGAGG AGAAGAAGGA CAGCAAGAAA 
GATGAGGTGC CAAAGAAGGA GGCTCCAAAG CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT 
GTGGAGAAAC CCAAAGAATC CAAAGTTGAA GCCAAGAAAG AGACTGAAGA TAAGAAAAAA 
GCAGTGACCC CAGAGAAGGA GGTTCCTGCC AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG 
AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT GATGCCAAGG CCAAAGAACC CAGCAAAGCA 
GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG GAAGGGACAC CTGCAGCACC CGAGAAAAAA 
GATGTCAAGG AGGAGAAGAC CCCAGAAGCC AAGAAATCTG AGGAGAAACC CAAAGCAGAG 
GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG GCCCCCACAC CTGGCAAAGC CAAGACAGAA 
AAGGCTGAGA AATCCTCTAG CACAGACCAA AAAGACAGCA GGCCTGCAGA GAAGGCCACA 
GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26008
Clone ID Related Accession (Same CDS sequence) XM_005636004.2
Accession Version XM_005636004.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAAGCCAAAT CCCCAGAGAA GGCCAAGTCT CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAAT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGCCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636061.1
CDS76..3447
Translation

Target ORF information:

RefSeq Version XM_005636004.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636004.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAAGCCAAAT CCCCAGAGAA GGCCAAGTCT CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAAT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGCCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26010
Clone ID Related Accession (Same CDS sequence) XM_005636006.2 , XM_005636006.3
Accession Version XM_005636006.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636063.1
CDS76..3279
Translation

Target ORF information:

RefSeq Version XM_005636006.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636006.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26010
Clone ID Related Accession (Same CDS sequence) XM_005636006.2 , XM_005636006.3
Accession Version XM_005636006.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636063.1
CDS71..3274
Translation

Target ORF information:

RefSeq Version XM_005636006.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636006.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26012
Clone ID Related Accession (Same CDS sequence) XM_005636008.2 , XM_005636008.3
Accession Version XM_005636008.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGAGAA GGTCAAATCT CCTGTGAAAG AAGAGACCAA GGCTCCTGAG 
AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG TCCCCCATAA AGGAGGAAGA GAAACCCCAG 
GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG GCAGAGGAAG AGAAAGCTCC AGCCACACCA 
AAAACTGAGG AGAAGAAGGA CAGCAAGAAA GATGAGGTGC CAAAGAAGGA GGCTCCAAAG 
CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT GTGGAGAAAC CCAAAGAATC CAAAGTTGAA 
GCCAAGAAAG AGACTGAAGA TAAGAAAAAA GCAGTGACCC CAGAGAAGGA GGTTCCTGCC 
AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT 
GATGCCAAGG CCAAAGAACC CAGCAAAGCA GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG 
GAAGGGACAC CTGCAGCACC CGAGAAAAAA GATGTCAAGG AGGAGAAGAC CCCAGAAGCC 
AAGAAATCTG AGGAGAAACC CAAAGCAGAG GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG 
GCCCCCACAC CTGGCAAAGC CAAGACAGAA AAGGCTGAGA AATCCTCTAG CACAGACCAA 
AAAGACAGCA GGCCTGCAGA GAAGGCCACA GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636065.1
CDS76..3135
Translation

Target ORF information:

RefSeq Version XM_005636008.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636008.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGAGAA GGTCAAATCT CCTGTGAAAG AAGAGACCAA GGCTCCTGAG 
AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG TCCCCCATAA AGGAGGAAGA GAAACCCCAG 
GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG GCAGAGGAAG AGAAAGCTCC AGCCACACCA 
AAAACTGAGG AGAAGAAGGA CAGCAAGAAA GATGAGGTGC CAAAGAAGGA GGCTCCAAAG 
CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT GTGGAGAAAC CCAAAGAATC CAAAGTTGAA 
GCCAAGAAAG AGACTGAAGA TAAGAAAAAA GCAGTGACCC CAGAGAAGGA GGTTCCTGCC 
AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT 
GATGCCAAGG CCAAAGAACC CAGCAAAGCA GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG 
GAAGGGACAC CTGCAGCACC CGAGAAAAAA GATGTCAAGG AGGAGAAGAC CCCAGAAGCC 
AAGAAATCTG AGGAGAAACC CAAAGCAGAG GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG 
GCCCCCACAC CTGGCAAAGC CAAGACAGAA AAGGCTGAGA AATCCTCTAG CACAGACCAA 
AAAGACAGCA GGCCTGCAGA GAAGGCCACA GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26012
Clone ID Related Accession (Same CDS sequence) XM_005636008.2 , XM_005636008.3
Accession Version XM_005636008.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGAGAA GGTCAAATCT CCTGTGAAAG AAGAGACCAA GGCTCCTGAG 
AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG TCCCCCATAA AGGAGGAAGA GAAACCCCAG 
GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG GCAGAGGAAG AGAAAGCTCC AGCCACACCA 
AAAACTGAGG AGAAGAAGGA CAGCAAGAAA GATGAGGTGC CAAAGAAGGA GGCTCCAAAG 
CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT GTGGAGAAAC CCAAAGAATC CAAAGTTGAA 
GCCAAGAAAG AGACTGAAGA TAAGAAAAAA GCAGTGACCC CAGAGAAGGA GGTTCCTGCC 
AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT 
GATGCCAAGG CCAAAGAACC CAGCAAAGCA GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG 
GAAGGGACAC CTGCAGCACC CGAGAAAAAA GATGTCAAGG AGGAGAAGAC CCCAGAAGCC 
AAGAAATCTG AGGAGAAACC CAAAGCAGAG GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG 
GCCCCCACAC CTGGCAAAGC CAAGACAGAA AAGGCTGAGA AATCCTCTAG CACAGACCAA 
AAAGACAGCA GGCCTGCAGA GAAGGCCACA GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636065.1
CDS71..3130
Translation

Target ORF information:

RefSeq Version XM_005636008.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636008.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG 
GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA 
AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG 
AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGAGAA GGTCAAATCT CCTGTGAAAG AAGAGACCAA GGCTCCTGAG 
AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG TCCCCCATAA AGGAGGAAGA GAAACCCCAG 
GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG GCAGAGGAAG AGAAAGCTCC AGCCACACCA 
AAAACTGAGG AGAAGAAGGA CAGCAAGAAA GATGAGGTGC CAAAGAAGGA GGCTCCAAAG 
CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT GTGGAGAAAC CCAAAGAATC CAAAGTTGAA 
GCCAAGAAAG AGACTGAAGA TAAGAAAAAA GCAGTGACCC CAGAGAAGGA GGTTCCTGCC 
AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT 
GATGCCAAGG CCAAAGAACC CAGCAAAGCA GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG 
GAAGGGACAC CTGCAGCACC CGAGAAAAAA GATGTCAAGG AGGAGAAGAC CCCAGAAGCC 
AAGAAATCTG AGGAGAAACC CAAAGCAGAG GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG 
GCCCCCACAC CTGGCAAAGC CAAGACAGAA AAGGCTGAGA AATCCTCTAG CACAGACCAA 
AAAGACAGCA GGCCTGCAGA GAAGGCCACA GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26013
Clone ID Related Accession (Same CDS sequence) XM_005636009.2 , XM_005636009.3
Accession Version XM_005636009.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG 
GTCACAAAGA AGGAAGAGGC AAAGTCCCCC ATAAAGGAGG AAGAGAAACC CCAGGAAGTG 
AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG GAAGAGAAAG CTCCAGCCAC ACCAAAAACT 
GAGGAGAAGA AGGACAGCAA GAAAGATGAG GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG 
GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG AAACCCAAAG AATCCAAAGT TGAAGCCAAG 
AAAGAGACTG AAGATAAGAA AAAAGCAGTG ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG 
AAGGAAGAAG CCAAACCCAA AGAGAAGGCT GAGGTGGCCA AGAAGGAGCA AGATGATGCC 
AAGGCCAAAG AACCCAGCAA AGCAGCAGAG AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG 
ACACCTGCAG CACCCGAGAA AAAAGATGTC AAGGAGGAGA AGACCCCAGA AGCCAAGAAA 
TCTGAGGAGA AACCCAAAGC AGAGGCCCCA GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC 
ACACCTGGCA AAGCCAAGAC AGAAAAGGCT GAGAAATCCT CTAGCACAGA CCAAAAAGAC 
AGCAGGCCTG CAGAGAAGGC CACAGAAGAC AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636066.1
CDS76..3009
Translation

Target ORF information:

RefSeq Version XM_005636009.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636009.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG 
GTCACAAAGA AGGAAGAGGC AAAGTCCCCC ATAAAGGAGG AAGAGAAACC CCAGGAAGTG 
AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG GAAGAGAAAG CTCCAGCCAC ACCAAAAACT 
GAGGAGAAGA AGGACAGCAA GAAAGATGAG GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG 
GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG AAACCCAAAG AATCCAAAGT TGAAGCCAAG 
AAAGAGACTG AAGATAAGAA AAAAGCAGTG ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG 
AAGGAAGAAG CCAAACCCAA AGAGAAGGCT GAGGTGGCCA AGAAGGAGCA AGATGATGCC 
AAGGCCAAAG AACCCAGCAA AGCAGCAGAG AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG 
ACACCTGCAG CACCCGAGAA AAAAGATGTC AAGGAGGAGA AGACCCCAGA AGCCAAGAAA 
TCTGAGGAGA AACCCAAAGC AGAGGCCCCA GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC 
ACACCTGGCA AAGCCAAGAC AGAAAAGGCT GAGAAATCCT CTAGCACAGA CCAAAAAGAC 
AGCAGGCCTG CAGAGAAGGC CACAGAAGAC AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26013
Clone ID Related Accession (Same CDS sequence) XM_005636009.2 , XM_005636009.3
Accession Version XM_005636009.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG 
GTCACAAAGA AGGAAGAGGC AAAGTCCCCC ATAAAGGAGG AAGAGAAACC CCAGGAAGTG 
AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG GAAGAGAAAG CTCCAGCCAC ACCAAAAACT 
GAGGAGAAGA AGGACAGCAA GAAAGATGAG GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG 
GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG AAACCCAAAG AATCCAAAGT TGAAGCCAAG 
AAAGAGACTG AAGATAAGAA AAAAGCAGTG ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG 
AAGGAAGAAG CCAAACCCAA AGAGAAGGCT GAGGTGGCCA AGAAGGAGCA AGATGATGCC 
AAGGCCAAAG AACCCAGCAA AGCAGCAGAG AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG 
ACACCTGCAG CACCCGAGAA AAAAGATGTC AAGGAGGAGA AGACCCCAGA AGCCAAGAAA 
TCTGAGGAGA AACCCAAAGC AGAGGCCCCA GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC 
ACACCTGGCA AAGCCAAGAC AGAAAAGGCT GAGAAATCCT CTAGCACAGA CCAAAAAGAC 
AGCAGGCCTG CAGAGAAGGC CACAGAAGAC AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636066.1
CDS71..3004
Translation

Target ORF information:

RefSeq Version XM_005636009.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636009.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG 
GTCACAAAGA AGGAAGAGGC AAAGTCCCCC ATAAAGGAGG AAGAGAAACC CCAGGAAGTG 
AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG GAAGAGAAAG CTCCAGCCAC ACCAAAAACT 
GAGGAGAAGA AGGACAGCAA GAAAGATGAG GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG 
GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG AAACCCAAAG AATCCAAAGT TGAAGCCAAG 
AAAGAGACTG AAGATAAGAA AAAAGCAGTG ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG 
AAGGAAGAAG CCAAACCCAA AGAGAAGGCT GAGGTGGCCA AGAAGGAGCA AGATGATGCC 
AAGGCCAAAG AACCCAGCAA AGCAGCAGAG AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG 
ACACCTGCAG CACCCGAGAA AAAAGATGTC AAGGAGGAGA AGACCCCAGA AGCCAAGAAA 
TCTGAGGAGA AACCCAAAGC AGAGGCCCCA GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC 
ACACCTGGCA AAGCCAAGAC AGAAAAGGCT GAGAAATCCT CTAGCACAGA CCAAAAAGAC 
AGCAGGCCTG CAGAGAAGGC CACAGAAGAC AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26014
Clone ID Related Accession (Same CDS sequence) XM_005636010.3 , XM_005636010.2
Accession Version XM_005636010.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636067.1
CDS71..2962
Translation

Target ORF information:

RefSeq Version XM_005636010.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636010.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26014
Clone ID Related Accession (Same CDS sequence) XM_005636010.3 , XM_005636010.2
Accession Version XM_005636010.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636067.1
CDS76..2967
Translation

Target ORF information:

RefSeq Version XM_005636010.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636010.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26015
Clone ID Related Accession (Same CDS sequence) XM_005636011.2
Accession Version XM_005636011.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636068.1
CDS76..2967
Translation

Target ORF information:

RefSeq Version XM_005636011.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636011.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCCGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AAAAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC 
AAGTCCCCAG AGAAGGCCAA GTCCCCTGAG AAAGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG AAGGTCAAAT CTCCTGTGAA AGAAGAGACC 
AAGGCTCCTG AGAAAGAGGT CACAAAGAAG GAAGAGGCAA AGTCCCCCAT AAAGGAGGAA 
GAGAAACCCC AGGAAGTGAA AGTCAAAGAG CCCGCAAAGA AGGCAGAGGA AGAGAAAGCT 
CCAGCCACAC CAAAAACTGA GGAGAAGAAG GACAGCAAGA AAGATGAGGT GCCAAAGAAG 
GAGGCTCCAA AGCCTGAGGT CCCCGAAAAG AAGGAGCCTG CTGTGGAGAA ACCCAAAGAA 
TCCAAAGTTG AAGCCAAGAA AGAGACTGAA GATAAGAAAA AAGCAGTGAC CCCAGAGAAG 
GAGGTTCCTG CCAAGGTGAA GGAAGAAGCC AAACCCAAAG AGAAGGCTGA GGTGGCCAAG 
AAGGAGCAAG ATGATGCCAA GGCCAAAGAA CCCAGCAAAG CAGCAGAGAA GGAGCCAGAA 
AAGCCAAAGA AGGAAGGGAC ACCTGCAGCA CCCGAGAAAA AAGATGTCAA GGAGGAGAAG 
ACCCCAGAAG CCAAGAAATC TGAGGAGAAA CCCAAAGCAG AGGCCCCAGC CAAAGAAGAG 
CCCAGCAAGG AGGCCCCCAC ACCTGGCAAA GCCAAGACAG AAAAGGCTGA GAAATCCTCT 
AGCACAGACC AAAAAGACAG CAGGCCTGCA GAGAAGGCCA CAGAAGACAA GGCCTCCAAG 
GGGGAGAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe26016
Clone ID Related Accession (Same CDS sequence) XM_005636012.2
Accession Version XM_005636012.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGTCAAATCT 
CCTGTGAAAG AAGAGACCAA GGCTCCTGAG AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG 
TCCCCCATAA AGGAGGAAGA GAAACCCCAG GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG 
GCAGAGGAAG AGAAAGCTCC AGCCACACCA AAAACTGAGG AGAAGAAGGA CAGCAAGAAA 
GATGAGGTGC CAAAGAAGGA GGCTCCAAAG CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT 
GTGGAGAAAC CCAAAGAATC CAAAGTTGAA GCCAAGAAAG AGACTGAAGA TAAGAAAAAA 
GCAGTGACCC CAGAGAAGGA GGTTCCTGCC AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG 
AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT GATGCCAAGG CCAAAGAACC CAGCAAAGCA 
GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG GAAGGGACAC CTGCAGCACC CGAGAAAAAA 
GATGTCAAGG AGGAGAAGAC CCCAGAAGCC AAGAAATCTG AGGAGAAACC CAAAGCAGAG 
GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG GCCCCCACAC CTGGCAAAGC CAAGACAGAA 
AAGGCTGAGA AATCCTCTAG CACAGACCAA AAAGACAGCA GGCCTGCAGA GAAGGCCACA 
GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005636069.1
CDS76..2925
Translation

Target ORF information:

RefSeq Version XM_005636012.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005636012.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCGGTGAA GGAGGAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCGG TGAAGGAGGA GGCCAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGAG GAGGCCAAGT CCCCCGAGAA GGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAA AAGGCCAAGT CCCCAGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCTGAGAA AGCCAAGTCC 
CCAGTGAAGG AGGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGTCAAATCT 
CCTGTGAAAG AAGAGACCAA GGCTCCTGAG AAAGAGGTCA CAAAGAAGGA AGAGGCAAAG 
TCCCCCATAA AGGAGGAAGA GAAACCCCAG GAAGTGAAAG TCAAAGAGCC CGCAAAGAAG 
GCAGAGGAAG AGAAAGCTCC AGCCACACCA AAAACTGAGG AGAAGAAGGA CAGCAAGAAA 
GATGAGGTGC CAAAGAAGGA GGCTCCAAAG CCTGAGGTCC CCGAAAAGAA GGAGCCTGCT 
GTGGAGAAAC CCAAAGAATC CAAAGTTGAA GCCAAGAAAG AGACTGAAGA TAAGAAAAAA 
GCAGTGACCC CAGAGAAGGA GGTTCCTGCC AAGGTGAAGG AAGAAGCCAA ACCCAAAGAG 
AAGGCTGAGG TGGCCAAGAA GGAGCAAGAT GATGCCAAGG CCAAAGAACC CAGCAAAGCA 
GCAGAGAAGG AGCCAGAAAA GCCAAAGAAG GAAGGGACAC CTGCAGCACC CGAGAAAAAA 
GATGTCAAGG AGGAGAAGAC CCCAGAAGCC AAGAAATCTG AGGAGAAACC CAAAGCAGAG 
GCCCCAGCCA AAGAAGAGCC CAGCAAGGAG GCCCCCACAC CTGGCAAAGC CAAGACAGAA 
AAGGCTGAGA AATCCTCTAG CACAGACCAA AAAGACAGCA GGCCTGCAGA GAAGGCCACA 
GAAGACAAGG CCTCCAAGGG GGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe43184
Clone ID Related Accession (Same CDS sequence) XM_014107712.2 , XM_014107712.1
Accession Version XM_014107712.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product neurofilament heavy polypeptide isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013963187.1
CDS71..3274
Translation

Target ORF information:

RefSeq Version XM_014107712.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neurofilament heavy (NEFH), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014107712.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe43184
Clone ID Related Accession (Same CDS sequence) XM_014107712.2 , XM_014107712.1
Accession Version XM_014107712.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product neurofilament heavy polypeptide isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013963187.1
CDS76..3279
Translation

Target ORF information:

RefSeq Version XM_014107712.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014107712.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGATGAGCT TCAGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTCCATGGA 
GGCGGCAGCC TGCACTACGC GTTGGCCCGC AAGGGCGGAA CGCGCTCTAC CGCCGGCTCA 
TCCAGTGGCT TCCACTCCTG GGCGCGGACA TCCGTGAGCT CCGTGTCGGC CTCTCCGAGC 
CGCTTCCGTG GCGCAGCAGC CACCTCAAGC ACCGACTCCC TAGACACGCT GAGTAACGGA 
CCGGAGGGCT GCGTGGTGGC AGCAGCCGCG GCCCGCAGCG AGAAGGAGCA GCTGCAGGCG 
CTGAATGACC GCTTCGCGGG CTACATCGAC AAGGTACGGC AGCTCGAGGC TCACAACCGC 
AGCCTGGAAG GCGAGGCGGC GGCCCTGCGG CAGCAGCACG CTGGCCGCGC CGCCATGGGC 
GAGCTGTACG AGCGAGAGGT GCGCGAGATG CGCGGCGCTG TGCTGCGCCT GGGCGCGGCG 
CGCGGCCAGC TGCGCCTGGA GCAGGAGCAC CTGCTCGAAG ACATTGCGCA CGTGCGCCAG 
CGCCTAGACG ACGAGGCCCG GCAGCGGGAA GAAGCTGAGG CGGCGGCGCG CGCACTCGCG 
CGCTTTGCGC AAGAGGCCGA GGCGGCGCGC GTCGAGCTGC AGAAGAAGGC GCAAGCGCTG 
CAGGAAGAGT GCGGCTACCT GCGGCGTCAC CACCAGGAGG AGGTGCGCGA GCTGCTCGGC 
CAGATCCAGG GCTGCGGCGC CGCGCAGGCG CAGGCGCAGG CCGAGGCGCG CGACGCCCTG 
AAGTGCGACG TGACGTCGGC GCTGCGCGAG ATCCGCGCGC AACTTGAAGG CCACGCGGTG 
CAGAGCACTC TGCAGTCGGA GGAGTGGTTC CGAGTGAGGC TGGACCGACT ATCAGAGGCA 
GCCAAGGTGA ACACAGACGC CATGCGCTCA GCCCAGGAGG AGATAACAGA GTATCGCCGG 
CAGCTGCAGG CTAGGACCAC AGAGCTGGAG GCACTCAAAG GCACCAAGGA CTCACTAGAG 
AGGCAGCGCT CTGAACTGGA GGACCGTCAT CAGGCTGACA TCGCATCCTA CCAGGAGGCC 
ATCCAGCAGC TGGACACTGA GCTGAGGAAC ACCAAGTGGG AGATGGCAGC CCAGCTCCGA 
GAGTACCAGG ACCTGCTCAA TGTCAAGATG GCTCTGGATA TTGAGATTGC CGCTTACAGA 
AAACTCCTGG AGGGTGAAGA ATGTCGGATT GGCTTTGGCC CCAGTCCTTT CTCCCTTCCA 
GAAGGACTCC CCAAAATTCC CTCTACATCC ACTCACATAA AGGTCAAAAG TGAAGAGAAG 
ATCAAAGTGG TAGAAAAGTC AGAGAAGGAA ACCGTGATTT TGGAGGAACA GACAGAGGAG 
ATCCAAGTGA CTGAAGAAGT GACTGAAGAA GAAGAGAAAG AGGCCAAAGA GGAGAAAGGT 
GAGGAAGAGG AAGCAGAAGA GGGAGAAGAA GAAACAAAGT CTCCCCCAGC AGAAGAGGCT 
GCATCTCCAG AGAAGGAAGA GGCCAAGTCC CCAGAAAAGG CTAAGTCCCC CATGAAAGAA 
GAAGCAAAAT CACCAGCTGA GGCCAAGTCC CCAGTGAAGG AAGAGGCCAA GTCTCCAGCT 
GAGGTGAAGT CCCCTGAGAA AGCTAAATCC CCCATGAAAG AAGAAGCAAA ATCTCCAACG 
GAGGTGAAAT CCCCAGAGAA GGCCAAGTCC CCAGCTAAGG AAGAAGCAAA GTCCCCTGTG 
GAGGCCAAGT CCCCCGAAAA GGCCAAGTCT CCAGTAAAGG AAGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGAGGA GGCCAAGTCC 
CCAGAGAAGG CCAAGTCCCC GGTGAAGGAG GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC 
CCGGTGAAGG AGGAGGCCAA GTCCCCAGAG AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC 
AAGTCCCCCG AGAAGACCAA GTCCCCAGTG AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC 
AAGTCCCCCG AGAAGGCCAA GTCTCCAGTG AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC 
AAGTCTCCAG TGAAGGAGGA GGCCAAGTCC CCTGAGAAGG CCAAGTCCCC GGTGAAGGAG 
GAGGCCAAGT CCCCTGAGAA GGCCAAGTCC CCGGTGAAGG AGGAGGCCAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAGGAGGCC AAGTCCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAAAAGGCC AAGTCCCCAG AGAAGGCCAA GTCCCCGGTG 
AAGGAGGAGG CCAAGTCCCC AGAGAAGGCC AAGTCCCCTG AGAAAGCCAA GTCCCCAGTG 
AAGGAGGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG AGAAGGTCAA ATCTCCTGTG 
AAAGAAGAGA CCAAGGCTCC TGAGAAAGAG GTCACAAAGA AGGAAGAGGC AAAGTCCCCC 
ATAAAGGAGG AAGAGAAACC CCAGGAAGTG AAAGTCAAAG AGCCCGCAAA GAAGGCAGAG 
GAAGAGAAAG CTCCAGCCAC ACCAAAAACT GAGGAGAAGA AGGACAGCAA GAAAGATGAG 
GTGCCAAAGA AGGAGGCTCC AAAGCCTGAG GTCCCCGAAA AGAAGGAGCC TGCTGTGGAG 
AAACCCAAAG AATCCAAAGT TGAAGCCAAG AAAGAGACTG AAGATAAGAA AAAAGCAGTG 
ACCCCAGAGA AGGAGGTTCC TGCCAAGGTG AAGGAAGAAG CCAAACCCAA AGAGAAGGCT 
GAGGTGGCCA AGAAGGAGCA AGATGATGCC AAGGCCAAAG AACCCAGCAA AGCAGCAGAG 
AAGGAGCCAG AAAAGCCAAA GAAGGAAGGG ACACCTGCAG CACCCGAGAA AAAAGATGTC 
AAGGAGGAGA AGACCCCAGA AGCCAAGAAA TCTGAGGAGA AACCCAAAGC AGAGGCCCCA 
GCCAAAGAAG AGCCCAGCAA GGAGGCCCCC ACACCTGGCA AAGCCAAGAC AGAAAAGGCT 
GAGAAATCCT CTAGCACAGA CCAAAAAGAC AGCAGGCCTG CAGAGAAGGC CACAGAAGAC 
AAGGCCTCCA AGGGGGAGAA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Combination of serum phosphorylated neurofilament heavy subunit and hyperintensity of intramedullary T2W on magnetic resonance imaging provides better prognostic value of canine thoracolumbar intervertebral disc herniation.
The Journal of veterinary medical science77(4)433-8(2015 Apr)
Mashita T,Kamishina H,Nakamoto Y,Akagi Y,Nakanishi A,Harasaki Y,Ozawa T,Uemura T,Kobatake Y,Shimamura S,Kitamura N,Maeda S,Uzuka Y,Shaw G,Yasuda J


book

Evaluation of serum phosphorylated neurofilament subunit NF-H as a prognostic biomarker in dogs with thoracolumbar intervertebral disc herniation.
Veterinary surgery : VS43(3)289-93(2014 Mar)
Nishida H,Nakayama M,Tanaka H,Kamishina H,Izawa T,Hatoya S,Sugiura K,Suzuki Y,Ide C,Inaba T


book

Allelic variants of the canine heavy neurofilament (NFH) subunit and extensive phosphorylation in dogs with motor neuron disease.
Journal of comparative pathology132(1)33-50(2005 Jan)
Green SL,Westendorf JM,Jaffe H,Pant HC,Cork LC,Ostrander EA,Vignaux F,Ferrell JE Jr