LIFR cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIFR
Entrez Gene ID	449478
Full Name	LIF receptor alpha
General protein information	Preferred Names LIF receptor alpha Names leukemia inhibitory factor receptor LIF receptor LIF-R leukemia inhibitory factor receptor alpha
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005760.1	NP_001005760.1	leukemia inhibitory factor receptor precursor
XM_005618875.2	XP_005618932.1	leukemia inhibitory factor receptor isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	lifr	XP_004910506.1
Pan troglodytes (chimpanzee)	LIFR	XP_001142123.1
Bos taurus (cattle)	LIFR	NP_001179192.1
Gallus gallus (chicken)	LIFR	NP_989906.1
Homo sapiens (human)	LIFR	NP_002301.1
Canis lupus familiaris (dog)	LIFR	NP_001005760.1
Rattus norvegicus (Norway rat)	Lifr	NP_112310.1
Danio rerio (zebrafish)	lifra	NP_001014328.1
Danio rerio (zebrafish)	lifrb	NP_001107204.1
	LIFR	XP_001082842.2
Mus musculus (house mouse)	Lifr	NP_038612.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of leukemia inhibitory factor and leukemia inhibitory factor receptor in the canine pituitary gland and corticotrope adenomas.
Hanson JM, Mol JA, Meij BP
Domestic animal endocrinology38(4)260-71(2010 May)

The leukemia inhibitory factor receptor gene is not involved in the etiology of pituitary dwarfism in German shepherd dogs.
Hanson JM, Mol JA, Leegwater PA, Kooistra HS, Meij BP
Research in veterinary science81(3)316-20(2006 Dec)

Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells.
Buk DM, Waibel M, Braig C, Martens AS, Heinrich PC, Graeve L
Journal of cell science117(Pt 10)2063-75(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data show that LIFR is highly co-expressed with adrenocorticotropic hormone (ACTH) and alpha-melanocyte-stimulating hormone (alpha-MSH) in the canine pituitary gland and in corticotrope adenomas
Title: Expression of leukemia inhibitory factor and leukemia inhibitory factor receptor in the canine pituitary gland and corticotrope adenomas.

There was no allelic association between a polymorphic microsatellite marker and pituitary dwarfism in German Shepherd dogs in this study.
Title: The leukemia inhibitory factor receptor gene is not involved in the etiology of pituitary dwarfism in German shepherd dogs.

each subunit of the CNTF receptor complex has a distinct distribution in polarised cells which may reflect the different roles the respective cytokines play in vivo
Title: Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells.

The following LIFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00106	NM_001005760.1 Latest version!	Canis lupus familiaris LIF receptor subunit alpha (LIFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04864	XM_005618875.2 Latest version!	Canis lupus familiaris leukemia inhibitory factor receptor alpha (LIFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIFR ( NM_001005760.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001005760.1 Canis lupus familiaris LIF receptor subunit alpha (LIFR), mRNA.

Price & Availability↑

CloneID	OCe00106
Clone ID Related Accession (Same CDS sequence)	NM_001005760.1
Accession Version	NM_001005760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-13
Organism	Canis lupus familiaris(dog)
Product	leukemia inhibitory factor receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY745241.1. On Jan 12, 2005 this sequence version replaced XM_536498.1. ##Evidence-Data-START## Transcript exon combination :: AY745241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGATGAACA TTTCCTTACG TTTGAGAAGA CCACCCTGGA TGGTGGATAG TAACGGACGG 
AGGATGACTT CACATTTCCA GTGGCTCTTA TTAACATTTA TTCTCCTGTA TTTAATGAAC 
CAAGTGACTA GTGAGAAAAG GGGGGCTCCT CGTGATTTGA AGTGTATAAC TAACAATTTG 
CGAGTATGGG ACTGTTCTTG GAAAGCACCC TCTGCAGCAG GCCATGGTAC TGTTTATGAA 
ATTTGCATCG AAAACAGGTC CCATTCTTGT TATCAATCAG AGAAAACAAA TACTAAAATT 
CCAGCTCTTT TACCTGGTGA TCATGAAATA ACAATAAACC GTCTGTATGA TTTCGGAAAT 
CCTATAAGTA AATTCACACT GAATGAAAAG AATGTTTCCT TAATTCCAGA CACGCCAGAG 
ATCTTGAATT TGTCTGCTGA TTTCTCAACT TCCACAATAC ACCTAAAGTG GAATGACAGG 
GGTTCTGTTT TTCCACATCA GTTAACTGTC ATCTGGGAAA TTAAAATTCT ACGTAAAGAA 
AATATGGAAA TCGTAAAATT AATAACTCAC AACACAACTG TGAATGGTAA AGATACAGTT 
CATCACTGGA GTTGGACCTC AGACATGCCC TTGGAGTGTG CCATTCATTC TGTGGGAATT 
AGATGCTATG TTGATGATCC TCATTTCTCT GGTCGCAAAG AGTGGAGTGA CTGGAGCCCA 
TTGAAGAACA TTTCTTGGTC ACCTGATTCT CAGACTAAGG TTTTCCCTCA AGACAAAGTG 
ATACTTGTAG GCTCAGATAT AACATTTTGT TGTGTGACTC AAGAAAAAGT GCTTTCAGCA 
CAGATTGGCC AAACGAATTG TCCCCTTATC CATCTTGATG GGGAAAATGT TGCAATCAAG 
ATCCATAATA TTTCTGTTTC TGCAAATAGT GGAACAAATG TGGTTTTCAC AACAGAAGAT 
AACATATTTG GGACGGTTAT TTTTGTAGGA TATCCACCTG ATATTCCTCA AAAAGTGAAT 
TGTGAGACAT ACGATTTAAA AGAGATTGTC TGTACTTGGA ATCCAGGAAG ACCAACCGCA 
TTGGTGGGCC CTCGCAATAC AAGTTATACT TTATTTGAAA GTTTCTCAGG GAAATATGTT 
AGATTTAAAA GAGTTGAAGC ACCAACAAAT GAAAGCTATC AATTATTCTT TCAAATGCGT 
CCAAATCAAG AAATCTATAA TTTTACTTTG AATGCTCGCA ATCCTCTGGG TCGATCAGAA 
TCAACTATTT TGATTAACAT AACTGAAAAA GTTTATCCTC GTATTCCTAC TTCAATCAAA 
GTGAAGGACA TTAATTCAAC AGCTGTTATG CTTTCCTGGC ATTTACCAGG CAACTTTGCA 
AAGATTAAAC TTTTATGTCA AATTGAAATT AATAAAACTA ACTCAGTACA AGAATTGCGG 
AATGTCACAA TCAAAGGAGT AGAAAATTCA AGTTACCTTG TAGCCGTGGA CAAGTTGAAT 
CCATATACTA TATATACTTT CCGGATTCGC TGTTCTACTG AAACTTTCTG GAAATGGAGC 
AAATGGAGCA ATGAAAAAAA ATATTTAACC ACAGAAGCCA TTCCTTCAAA GGGACCAGAT 
ACTTGGAGAG AGTGGAGTTC TGATGGGAAA AATTTAATCA TCTATTGGAA GCCTTTACCC 
ATTAATGAAG CTAATGGGAA AATACTTTCC TATAATGTAT CATGTTCACT AGATGAAGAG 
ACACAGTCCC TTTCTGAGAT CCCTGATCCT CAACACAAGG CGGAACTTCA ACTTGATAAA 
AATGACTATA TCATCAGTGT GGTGGCAAAA AATTCTGTTG GCTCATCGCC ACCTTCTAAA 
ATAGCTAGTA TGGAAATTCC AAATGATGAT CTCAAAGTAG AGCAGGCTGT CGGAATGGGA 
AATGGGATTC TCCTCACTTG GAATTACGAT CCCAATATGA CCTGTGACTA TGTTATTAAA 
TGGTGTAACT CATCTCGATC TGAACCCTGC CTTATGGACT GGAAAAAAGT CCCCTCAAAC 
AGCACTGAAG CTGTAATAGA ATCTGATCAG TTTCGACCAG GTGTAAGATA CAGTTTTTTC 
CTGTATGGGT GCAGGAATGA AGGATATCAG TTATTACGTT CTATAATCGG ATATATAGAA 
GAACTGGCTC CAATTGTGGC GCCAAATTTT ACTGTTGAGG ATACATCTGC AGATTCAATA 
TTAGTAAAAT GGGAAGATAT TCCTGTGGAA GAGCTCAGAG GCTTTTTAAG AGGATATTTA 
TTTTACTTTG AAAAAGGAGA GAGAGACACC TCTAAGATAA GGGGTTTGGA GTCAGGTCGG 
TCCGACATAA AGGTGAAGAA CATTACTGAC CTATCCCAGA AGACCCTGAG AATTGCTGAT 
CTTCAGGGTA AAACAAGTTA CCATCTGGTC TTACGAGCCT ATACAGACGG TGGGATGGGC 
CCGGAGAAGA GCATGTTTGT GGTGACAAAG GAGAATTCTG TGGGATTAAT TATTGCCATT 
CTCATTCCTG TGGCCGTGGC TGTCATTGTG GGAGTGGTAA CAAGTATCCT TTGCTATCGG 
AAACGAGAAT GGATTAAAGA AACCTTCTAC CCTGATATTC CAAATCCAGA AAATTGTAAA 
GCTTTACAGT TTCAAAAGAG TGTCTGTGAG GGAAACAGTG CTCTCAAAAC ATTGGAAATG 
AATCCTTGTA CCCCAAATAA CGTTGAGGTT CTGGAGACTC GATCAGCAGT TCCGAAAATA 
GAAGACACAG AAATAATCTC TCCCATAGCT GAGCGTCCGG AAGAGAGCTC TGATGCGGAA 
GCTGAGAACC ACGTGGTGGT GTCCTACTGC CCCCCTGTCA TTGAGGAGGA AACGCCGAAC 
CCCGGCGCAG ATGAGGCGGG GGGAGCCTCT CAAGTCGTTT ACATTGACAT CCAGTCTATG 
TACCAGCCTC AAGCGAAACC AGAGGAAGAA CAGGAAAATG ACCCTGTCGG GGGGGCCGGC 
TACAAGCCAC AGATGCACCT CCCCGTCACT TCTACTGTAG AAGACCTCGC TGCAGAGGAC 
GACCTAGATA AAGCTGCGGG TTACAGACCT CAGGCAAATG TCAACACTTG GAACTTAGTA 
TCTCCAGACT CCCCCAGGTC CACGGACAGC AACAGTGAAA TTGTCTCCTT TGGAAGCCCC 
TGCTCCATTA ACTCCCGACA GTTTCTGATT CCTCCTAAAG ATGAAGACTC TCCGAAATCC 
AGTGGGGGAG GGTGGTCCTT TACAAACTTC TTTCAGAACA AACCAAATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005760.1
CDS	1..3294
Translation	MMNISLRLRRPPWMVDSNGRRMTSHFQWLLLTFILLYLMNQVTSEKRGAPRDLKCITNNLRVWDCSWKAPSAAGHGTVYEICIENRSHSCYQSEKTNTKIPALLPGDHEITINRLYDFGNPISKFTLNEKNVSLIPDTPEILNLSADFSTSTIHLKWNDRGSVFPHQLTVIWEIKILRKENMEIVKLITHNTTVNGKDTVHHWSWTSDMPLECAIHSVGIRCYVDDPHFSGRKEWSDWSPLKNISWSPDSQTKVFPQDKVILVGSDITFCCVTQEKVLSAQIGQTNCPLIHLDGENVAIKIHNISVSANSGTNVVFTTEDNIFGTVIFVGYPPDIPQKVNCETYDLKEIVCTWNPGRPTALVGPRNTSYTLFESFSGKYVRFKRVEAPTNESYQLFFQMRPNQEIYNFTLNARNPLGRSESTILINITEKVYPRIPTSIKVKDINSTAVMLSWHLPGNFAKIKLLCQIEINKTNSVQELRNVTIKGVENSSYLVAVDKLNPYTIYTFRIRCSTETFWKWSKWSNEKKYLTTEAIPSKGPDTWREWSSDGKNLIIYWKPLPINEANGKILSYNVSCSLDEETQSLSEIPDPQHKAELQLDKNDYIISVVAKNSVGSSPPSKIASMEIPNDDLKVEQAVGMGNGILLTWNYDPNMTCDYVIKWCNSSRSEPCLMDWKKVPSNSTEAVIESDQFRPGVRYSFFLYGCRNEGYQLLRSIIGYIEELAPIVAPNFTVEDTSADSILVKWEDIPVEELRGFLRGYLFYFEKGERDTSKIRGLESGRSDIKVKNITDLSQKTLRIADLQGKTSYHLVLRAYTDGGMGPEKSMFVVTKENSVGLIIAILIPVAVAVIVGVVTSILCYRKREWIKETFYPDIPNPENCKALQFQKSVCEGNSALKTLEMNPCTPNNVEVLETRSAVPKIEDTEIISPIAERPEESSDAEAENHVVVSYCPPVIEEETPNPGADEAGGASQVVYIDIQSMYQPQAKPEEEQENDPVGGAGYKPQMHLPVTSTVEDLAAEDDLDKAAGYRPQANVNTWNLVSPDSPRSTDSNSEIVSFGSPCSINSRQFLIPPKDEDSPKSSGGGWSFTNFFQNKPND

Target ORF information:

RefSeq Version	NM_001005760.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris LIF receptor subunit alpha (LIFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005760.1

ORF Insert Sequence:

ATGATGAACA TTTCCTTACG TTTGAGAAGA CCACCCTGGA TGGTGGATAG TAACGGACGG 
AGGATGACTT CACATTTCCA GTGGCTCTTA TTAACATTTA TTCTCCTGTA TTTAATGAAC 
CAAGTGACTA GTGAGAAAAG GGGGGCTCCT CGTGATTTGA AGTGTATAAC TAACAATTTG 
CGAGTATGGG ACTGTTCTTG GAAAGCACCC TCTGCAGCAG GCCATGGTAC TGTTTATGAA 
ATTTGCATCG AAAACAGGTC CCATTCTTGT TATCAATCAG AGAAAACAAA TACTAAAATT 
CCAGCTCTTT TACCTGGTGA TCATGAAATA ACAATAAACC GTCTGTATGA TTTCGGAAAT 
CCTATAAGTA AATTCACACT GAATGAAAAG AATGTTTCCT TAATTCCAGA CACGCCAGAG 
ATCTTGAATT TGTCTGCTGA TTTCTCAACT TCCACAATAC ACCTAAAGTG GAATGACAGG 
GGTTCTGTTT TTCCACATCA GTTAACTGTC ATCTGGGAAA TTAAAATTCT ACGTAAAGAA 
AATATGGAAA TCGTAAAATT AATAACTCAC AACACAACTG TGAATGGTAA AGATACAGTT 
CATCACTGGA GTTGGACCTC AGACATGCCC TTGGAGTGTG CCATTCATTC TGTGGGAATT 
AGATGCTATG TTGATGATCC TCATTTCTCT GGTCGCAAAG AGTGGAGTGA CTGGAGCCCA 
TTGAAGAACA TTTCTTGGTC ACCTGATTCT CAGACTAAGG TTTTCCCTCA AGACAAAGTG 
ATACTTGTAG GCTCAGATAT AACATTTTGT TGTGTGACTC AAGAAAAAGT GCTTTCAGCA 
CAGATTGGCC AAACGAATTG TCCCCTTATC CATCTTGATG GGGAAAATGT TGCAATCAAG 
ATCCATAATA TTTCTGTTTC TGCAAATAGT GGAACAAATG TGGTTTTCAC AACAGAAGAT 
AACATATTTG GGACGGTTAT TTTTGTAGGA TATCCACCTG ATATTCCTCA AAAAGTGAAT 
TGTGAGACAT ACGATTTAAA AGAGATTGTC TGTACTTGGA ATCCAGGAAG ACCAACCGCA 
TTGGTGGGCC CTCGCAATAC AAGTTATACT TTATTTGAAA GTTTCTCAGG GAAATATGTT 
AGATTTAAAA GAGTTGAAGC ACCAACAAAT GAAAGCTATC AATTATTCTT TCAAATGCGT 
CCAAATCAAG AAATCTATAA TTTTACTTTG AATGCTCGCA ATCCTCTGGG TCGATCAGAA 
TCAACTATTT TGATTAACAT AACTGAAAAA GTTTATCCTC GTATTCCTAC TTCAATCAAA 
GTGAAGGACA TTAATTCAAC AGCTGTTATG CTTTCCTGGC ATTTACCAGG CAACTTTGCA 
AAGATTAAAC TTTTATGTCA AATTGAAATT AATAAAACTA ACTCAGTACA AGAATTGCGG 
AATGTCACAA TCAAAGGAGT AGAAAATTCA AGTTACCTTG TAGCCGTGGA CAAGTTGAAT 
CCATATACTA TATATACTTT CCGGATTCGC TGTTCTACTG AAACTTTCTG GAAATGGAGC 
AAATGGAGCA ATGAAAAAAA ATATTTAACC ACAGAAGCCA TTCCTTCAAA GGGACCAGAT 
ACTTGGAGAG AGTGGAGTTC TGATGGGAAA AATTTAATCA TCTATTGGAA GCCTTTACCC 
ATTAATGAAG CTAATGGGAA AATACTTTCC TATAATGTAT CATGTTCACT AGATGAAGAG 
ACACAGTCCC TTTCTGAGAT CCCTGATCCT CAACACAAGG CGGAACTTCA ACTTGATAAA 
AATGACTATA TCATCAGTGT GGTGGCAAAA AATTCTGTTG GCTCATCGCC ACCTTCTAAA 
ATAGCTAGTA TGGAAATTCC AAATGATGAT CTCAAAGTAG AGCAGGCTGT CGGAATGGGA 
AATGGGATTC TCCTCACTTG GAATTACGAT CCCAATATGA CCTGTGACTA TGTTATTAAA 
TGGTGTAACT CATCTCGATC TGAACCCTGC CTTATGGACT GGAAAAAAGT CCCCTCAAAC 
AGCACTGAAG CTGTAATAGA ATCTGATCAG TTTCGACCAG GTGTAAGATA CAGTTTTTTC 
CTGTATGGGT GCAGGAATGA AGGATATCAG TTATTACGTT CTATAATCGG ATATATAGAA 
GAACTGGCTC CAATTGTGGC GCCAAATTTT ACTGTTGAGG ATACATCTGC AGATTCAATA 
TTAGTAAAAT GGGAAGATAT TCCTGTGGAA GAGCTCAGAG GCTTTTTAAG AGGATATTTA 
TTTTACTTTG AAAAAGGAGA GAGAGACACC TCTAAGATAA GGGGTTTGGA GTCAGGTCGG 
TCCGACATAA AGGTGAAGAA CATTACTGAC CTATCCCAGA AGACCCTGAG AATTGCTGAT 
CTTCAGGGTA AAACAAGTTA CCATCTGGTC TTACGAGCCT ATACAGACGG TGGGATGGGC 
CCGGAGAAGA GCATGTTTGT GGTGACAAAG GAGAATTCTG TGGGATTAAT TATTGCCATT 
CTCATTCCTG TGGCCGTGGC TGTCATTGTG GGAGTGGTAA CAAGTATCCT TTGCTATCGG 
AAACGAGAAT GGATTAAAGA AACCTTCTAC CCTGATATTC CAAATCCAGA AAATTGTAAA 
GCTTTACAGT TTCAAAAGAG TGTCTGTGAG GGAAACAGTG CTCTCAAAAC ATTGGAAATG 
AATCCTTGTA CCCCAAATAA CGTTGAGGTT CTGGAGACTC GATCAGCAGT TCCGAAAATA 
GAAGACACAG AAATAATCTC TCCCATAGCT GAGCGTCCGG AAGAGAGCTC TGATGCGGAA 
GCTGAGAACC ACGTGGTGGT GTCCTACTGC CCCCCTGTCA TTGAGGAGGA AACGCCGAAC 
CCCGGCGCAG ATGAGGCGGG GGGAGCCTCT CAAGTCGTTT ACATTGACAT CCAGTCTATG 
TACCAGCCTC AAGCGAAACC AGAGGAAGAA CAGGAAAATG ACCCTGTCGG GGGGGCCGGC 
TACAAGCCAC AGATGCACCT CCCCGTCACT TCTACTGTAG AAGACCTCGC TGCAGAGGAC 
GACCTAGATA AAGCTGCGGG TTACAGACCT CAGGCAAATG TCAACACTTG GAACTTAGTA 
TCTCCAGACT CCCCCAGGTC CACGGACAGC AACAGTGAAA TTGTCTCCTT TGGAAGCCCC 
TGCTCCATTA ACTCCCGACA GTTTCTGATT CCTCCTAAAG ATGAAGACTC TCCGAAATCC 
AGTGGGGGAG GGTGGTCCTT TACAAACTTC TTTCAGAACA AACCAAATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIFR ( XM_005618875.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005618932.1 Canis lupus familiaris leukemia inhibitory factor receptor alpha (LIFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe04864
Clone ID Related Accession (Same CDS sequence)	XM_005618875.2
Accession Version	XM_005618875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	leukemia inhibitory factor receptor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGTG TGCATATACA GGCTCCTAGG ATGAACATCT CAAATCCCAA CCTCTCTCCC 
AGAAGCTGTG TCTCTACAGA ACTGTGTTGG CCGAGACTGC TCTCAACTGA ATTGGGCCCA 
GCTCAGAGAG GCAGCTGCTG GGTATCAACT CAGCTTCCAG GACTGACTAC TTTTGCACGG 
ATGATGAACA TTTCCTTACG TTTGAGAAGA CCACCCTGGA TGGTGGATAG TAACGGACGG 
AGGATGACTT CACATTTCCA GTGGCTCTTA TTAACATTTA TTCTCCTGTA TTTAATGAAC 
CAAGTGACTA GTGAGAAAAG GGGGGCTCCT CGTGATTTGA AGTGTATAAC TAACAATTTG 
CGAGTATGGG ACTGTTCTTG GAAAGCACCC TCTGCAGCAG GCCATGGTAC TGTTTATGAA 
ATTTGCATCG AAAACAGGTC CCATTCTTGT TATCAATCAG AGAAAACAAA TACTAAAATT 
CCAGCTCTTT TACCTGGTGA TCATGAAATA ACAATAAACC GTCTGTATGA TTTCGGAAAT 
CCTATAAGTA AATTCACACT GAATGAAAAG AATGTTTCCT TAATTCCAGA CACGCCAGAG 
ATCTTGAATT TGTCTGCTGA TTTCTCAACT TCCACAATAC ACCTAAAGTG GAATGACAGG 
GGTTCTGTTT TTCCACATCA GTTAACTGTC ATCTGGGAAA TTAAAATTCT ACGTAAAGAA 
AATATGGAAA TCGTAAAATT AATAACTCAC AACACAACTG TGAATGGTAA AGATACAGTT 
CATCACTGGA GTTGGACCTC AGACATGCCC TTGGAGTGTG CCATTCATTC TGTGGGAATT 
AGATGCTATG TTGATGATCC TCATTTCTCT GGTCGCAAAG AGTGGAGTGA CTGGAGCCCA 
TTGAAGAACA TTTCTTGGTC ACCTGATTCT CAGACTAAGG TTTTCCCTCA AGACAAAGTG 
ATACTTGTAG GCTCAGATAT AACATTTTGT TGTGTGACTC AAGAAAAAGT GCTTTCAGCA 
CAGATTGGCC AAACGAATTG TCCCCTTATC CATCTTGATG GGGAAAATGT TGCAATCAAG 
ATCCATAATA TTTCTGTTTC TGCAAATAGT GGAACAAATG TGGTTTTCAC AACAGAAGAT 
AACATATTTG GGACGGTTAT TTTTGTAGGA TATCCACCTG ATATTCCTCA AAAAGTGAAT 
TGTGAGACAT ACGATTTAAA AGAGATTGTC TGTACTTGGA ATCCAGGAAG ACCAACCGCA 
TTGGTGGGCC CTCGCAATAC AAGTTATACT TTATTTGAAA GTTTCTCAGG GAAATATGTT 
AGATTTAAAA GAGTTGAAGC ACCAACAAAT GAAAGCTATC AATTATTCTT TCAAATGCGT 
CCAAATCAAG AAATCTATAA TTTTACTTTG AATGCTCGCA ATCCTCTGGG TCGATCAGAA 
TCAACTATTT TGATTAACAT AACTGAAAAA GTTTATCCTC GTATTCCTAC TTCAATCAAA 
GTGAAGGACA TTAATTCAAC AGCTGTTATG CTTTCCTGGC ATTTACCAGG CAACTTTGCA 
AAGATTAAAC TTTTATGTCA AATTGAAATT AATAAAACTA ACTCAGTACA AGAATTGCGG 
AATGTCACAA TCAAAGGAGT AGAAAATTCA AGTTACCTTG TAGCCGTGGA CAAGTTGAAT 
CCATATACTA TATATACTTT CCGGATTCGC TGTTCTACTG AAACTTTCTG GAAATGGAGC 
AAATGGAGCA ATGAAAAAAA ATATTTAACC ACAGAAGCCA TTCCTTCAAA GGGACCAGAT 
ACTTGGAGAG AGTGGAGTTC TGATGGGAAA AATTTAATCA TCTATTGGAA GCCTTTACCC 
ATTAATGAAG CTAATGGGAA AATACTTTCC TATAATGTAT CATGTTCACT AGATGAAGAG 
ACACAGTCCC TTTCTGAGAT CCCTGATCCT CAACACAAGG CGGAACTTCA ACTTGATAAA 
AATGACTATA TCATCAGTGT GGTGGCAAAA AATTCTGTTG GCTCATCGCC ACCTTCTAAA 
ATAGCTAGTA TGGAAATTCC AAATGATGAT CTCAAAGTAG AGCAGGCTGT CGGAATGGGA 
AATGGGATTC TCCTCACTTG GAATTACGAT CCCAATATGA CCTGTGACTA TGTTATTAAA 
TGGTGTAACT CATCTCGATC TGAACCCTGC CTTATGGACT GGAAAAAAGT CCCCTCAAAC 
AGCACTGAAG CTGTAATAGA ATCTGATCAG TTTCGACCAG GTGTAAGATA CAGTTTTTTC 
CTGTATGGGT GCAGGAATGA AGGATATCAG TTATTACGTT CTATAATCGG ATATATAGAA 
GAACTGGCTC CAATTGTGGC GCCAAATTTT ACTGTTGAGG ATACATCTGC AGATTCAATA 
TTAGTAAAAT GGGAAGATAT TCCTGTGGAA GAGCTCAGAG GCTTTTTAAG AGGATATTTA 
TTTTACTTTG AAAAAGGAGA GAGAGACACC TCTAAGATAA GGGGTTTGGA GTCAGGTCGG 
TCCGACATAA AGGTGAAGAA CATTACTGAC CTATCCCAGA AGACCCTGAG AATTGCTGAT 
CTTCAGGGTA AAACAAGTTA CCATCTGGTC TTACGAGCCT ATACAGACGG TGGGATGGGC 
CCGGAGAAGA GCATGTTTGT GGTGACAAAG GAGAATTCTG TGGGATTAAT TATTGCCATT 
CTCATTCCTG TGGCCGTGGC TGTCATTGTG GGAGTGGTAA CAAGTATCCT TTGCTATCGG 
AAACGAGAAT GGATTAAAGA AACCTTCTAC CCTGATATTC CAAATCCAGA AAATTGTAAA 
GCTTTACAGT TTCAAAAGAG TGTCTGTGAG GGAAACAGTG CTCTCAAAAC ATTGGAAATG 
AATCCTTGTA CCCCAAATAA CGTTGAGGTT CTGGAGACTC GATCAGCAGT TCCGAAAATA 
GAAGACACAG AAATAATCTC TCCCATAGCT GAGCGTCCGG AAGAGAGCTC TGATGCGGAA 
GCTGAGAACC ACGTGGTGGT GTCCTACTGC CCCCCTGTCA TTGAGGAGGA AACGCCGAAC 
CCCGGCGCAG ATGAGGCGGG GGGAGCCTCT CAAGTCGTTT ACATTGACAT CCAGTCTATG 
TACCAGCCTC AAGCGAAACC AGAGGAAGAA CAGGAAAATG ACCCTGTCGG GGGGGCCGGC 
TACAAGCCAC AGATGCACCT CCCCGTCACT TCTACTGTAG AAGACCTCGC TGCAGAGGAC 
GACCTAGATA AAGCTGCGGG TTACAGACCT CAGGCAAATG TCAACACTTG GAACTTAGTA 
TCTCCAGACT CCCCCAGGTC CACGGACAGC AACAGTGAAA TTGTCTCCTT TGGAAGCCCC 
TGCTCCATTA ACTCCCGACA GTTTCTGATT CCTCCTAAAG ATGAAGACTC TCCGAAATCC 
AGTGGGGGAG GGTGGTCCTT TACAAACTTC TTTCAGAACA AACCAAATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618932.1
CDS	157..3630
Translation	MPCVHIQAPRMNISNPNLSPRSCVSTELCWPRLLSTELGPAQRGSCWVSTQLPGLTTFARMMNISLRLRRPPWMVDSNGRRMTSHFQWLLLTFILLYLMNQVTSEKRGAPRDLKCITNNLRVWDCSWKAPSAAGHGTVYEICIENRSHSCYQSEKTNTKIPALLPGDHEITINRLYDFGNPISKFTLNEKNVSLIPDTPEILNLSADFSTSTIHLKWNDRGSVFPHQLTVIWEIKILRKENMEIVKLITHNTTVNGKDTVHHWSWTSDMPLECAIHSVGIRCYVDDPHFSGRKEWSDWSPLKNISWSPDSQTKVFPQDKVILVGSDITFCCVTQEKVLSAQIGQTNCPLIHLDGENVAIKIHNISVSANSGTNVVFTTEDNIFGTVIFVGYPPDIPQKVNCETYDLKEIVCTWNPGRPTALVGPRNTSYTLFESFSGKYVRFKRVEAPTNESYQLFFQMRPNQEIYNFTLNARNPLGRSESTILINITEKVYPRIPTSIKVKDINSTAVMLSWHLPGNFAKIKLLCQIEINKTNSVQELRNVTIKGVENSSYLVAVDKLNPYTIYTFRIRCSTETFWKWSKWSNEKKYLTTEAIPSKGPDTWREWSSDGKNLIIYWKPLPINEANGKILSYNVSCSLDEETQSLSEIPDPQHKAELQLDKNDYIISVVAKNSVGSSPPSKIASMEIPNDDLKVEQAVGMGNGILLTWNYDPNMTCDYVIKWCNSSRSEPCLMDWKKVPSNSTEAVIESDQFRPGVRYSFFLYGCRNEGYQLLRSIIGYIEELAPIVAPNFTVEDTSADSILVKWEDIPVEELRGFLRGYLFYFEKGERDTSKIRGLESGRSDIKVKNITDLSQKTLRIADLQGKTSYHLVLRAYTDGGMGPEKSMFVVTKENSVGLIIAILIPVAVAVIVGVVTSILCYRKREWIKETFYPDIPNPENCKALQFQKSVCEGNSALKTLEMNPCTPNNVEVLETRSAVPKIEDTEIISPIAERPEESSDAEAENHVVVSYCPPVIEEETPNPGADEAGGASQVVYIDIQSMYQPQAKPEEEQENDPVGGAGYKPQMHLPVTSTVEDLAAEDDLDKAAGYRPQANVNTWNLVSPDSPRSTDSNSEIVSFGSPCSINSRQFLIPPKDEDSPKSSGGGWSFTNFFQNKPND

Target ORF information:

RefSeq Version	XM_005618875.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leukemia inhibitory factor receptor alpha (LIFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618875.2

ORF Insert Sequence:

ATGCCATGTG TGCATATACA GGCTCCTAGG ATGAACATCT CAAATCCCAA CCTCTCTCCC 
AGAAGCTGTG TCTCTACAGA ACTGTGTTGG CCGAGACTGC TCTCAACTGA ATTGGGCCCA 
GCTCAGAGAG GCAGCTGCTG GGTATCAACT CAGCTTCCAG GACTGACTAC TTTTGCACGG 
ATGATGAACA TTTCCTTACG TTTGAGAAGA CCACCCTGGA TGGTGGATAG TAACGGACGG 
AGGATGACTT CACATTTCCA GTGGCTCTTA TTAACATTTA TTCTCCTGTA TTTAATGAAC 
CAAGTGACTA GTGAGAAAAG GGGGGCTCCT CGTGATTTGA AGTGTATAAC TAACAATTTG 
CGAGTATGGG ACTGTTCTTG GAAAGCACCC TCTGCAGCAG GCCATGGTAC TGTTTATGAA 
ATTTGCATCG AAAACAGGTC CCATTCTTGT TATCAATCAG AGAAAACAAA TACTAAAATT 
CCAGCTCTTT TACCTGGTGA TCATGAAATA ACAATAAACC GTCTGTATGA TTTCGGAAAT 
CCTATAAGTA AATTCACACT GAATGAAAAG AATGTTTCCT TAATTCCAGA CACGCCAGAG 
ATCTTGAATT TGTCTGCTGA TTTCTCAACT TCCACAATAC ACCTAAAGTG GAATGACAGG 
GGTTCTGTTT TTCCACATCA GTTAACTGTC ATCTGGGAAA TTAAAATTCT ACGTAAAGAA 
AATATGGAAA TCGTAAAATT AATAACTCAC AACACAACTG TGAATGGTAA AGATACAGTT 
CATCACTGGA GTTGGACCTC AGACATGCCC TTGGAGTGTG CCATTCATTC TGTGGGAATT 
AGATGCTATG TTGATGATCC TCATTTCTCT GGTCGCAAAG AGTGGAGTGA CTGGAGCCCA 
TTGAAGAACA TTTCTTGGTC ACCTGATTCT CAGACTAAGG TTTTCCCTCA AGACAAAGTG 
ATACTTGTAG GCTCAGATAT AACATTTTGT TGTGTGACTC AAGAAAAAGT GCTTTCAGCA 
CAGATTGGCC AAACGAATTG TCCCCTTATC CATCTTGATG GGGAAAATGT TGCAATCAAG 
ATCCATAATA TTTCTGTTTC TGCAAATAGT GGAACAAATG TGGTTTTCAC AACAGAAGAT 
AACATATTTG GGACGGTTAT TTTTGTAGGA TATCCACCTG ATATTCCTCA AAAAGTGAAT 
TGTGAGACAT ACGATTTAAA AGAGATTGTC TGTACTTGGA ATCCAGGAAG ACCAACCGCA 
TTGGTGGGCC CTCGCAATAC AAGTTATACT TTATTTGAAA GTTTCTCAGG GAAATATGTT 
AGATTTAAAA GAGTTGAAGC ACCAACAAAT GAAAGCTATC AATTATTCTT TCAAATGCGT 
CCAAATCAAG AAATCTATAA TTTTACTTTG AATGCTCGCA ATCCTCTGGG TCGATCAGAA 
TCAACTATTT TGATTAACAT AACTGAAAAA GTTTATCCTC GTATTCCTAC TTCAATCAAA 
GTGAAGGACA TTAATTCAAC AGCTGTTATG CTTTCCTGGC ATTTACCAGG CAACTTTGCA 
AAGATTAAAC TTTTATGTCA AATTGAAATT AATAAAACTA ACTCAGTACA AGAATTGCGG 
AATGTCACAA TCAAAGGAGT AGAAAATTCA AGTTACCTTG TAGCCGTGGA CAAGTTGAAT 
CCATATACTA TATATACTTT CCGGATTCGC TGTTCTACTG AAACTTTCTG GAAATGGAGC 
AAATGGAGCA ATGAAAAAAA ATATTTAACC ACAGAAGCCA TTCCTTCAAA GGGACCAGAT 
ACTTGGAGAG AGTGGAGTTC TGATGGGAAA AATTTAATCA TCTATTGGAA GCCTTTACCC 
ATTAATGAAG CTAATGGGAA AATACTTTCC TATAATGTAT CATGTTCACT AGATGAAGAG 
ACACAGTCCC TTTCTGAGAT CCCTGATCCT CAACACAAGG CGGAACTTCA ACTTGATAAA 
AATGACTATA TCATCAGTGT GGTGGCAAAA AATTCTGTTG GCTCATCGCC ACCTTCTAAA 
ATAGCTAGTA TGGAAATTCC AAATGATGAT CTCAAAGTAG AGCAGGCTGT CGGAATGGGA 
AATGGGATTC TCCTCACTTG GAATTACGAT CCCAATATGA CCTGTGACTA TGTTATTAAA 
TGGTGTAACT CATCTCGATC TGAACCCTGC CTTATGGACT GGAAAAAAGT CCCCTCAAAC 
AGCACTGAAG CTGTAATAGA ATCTGATCAG TTTCGACCAG GTGTAAGATA CAGTTTTTTC 
CTGTATGGGT GCAGGAATGA AGGATATCAG TTATTACGTT CTATAATCGG ATATATAGAA 
GAACTGGCTC CAATTGTGGC GCCAAATTTT ACTGTTGAGG ATACATCTGC AGATTCAATA 
TTAGTAAAAT GGGAAGATAT TCCTGTGGAA GAGCTCAGAG GCTTTTTAAG AGGATATTTA 
TTTTACTTTG AAAAAGGAGA GAGAGACACC TCTAAGATAA GGGGTTTGGA GTCAGGTCGG 
TCCGACATAA AGGTGAAGAA CATTACTGAC CTATCCCAGA AGACCCTGAG AATTGCTGAT 
CTTCAGGGTA AAACAAGTTA CCATCTGGTC TTACGAGCCT ATACAGACGG TGGGATGGGC 
CCGGAGAAGA GCATGTTTGT GGTGACAAAG GAGAATTCTG TGGGATTAAT TATTGCCATT 
CTCATTCCTG TGGCCGTGGC TGTCATTGTG GGAGTGGTAA CAAGTATCCT TTGCTATCGG 
AAACGAGAAT GGATTAAAGA AACCTTCTAC CCTGATATTC CAAATCCAGA AAATTGTAAA 
GCTTTACAGT TTCAAAAGAG TGTCTGTGAG GGAAACAGTG CTCTCAAAAC ATTGGAAATG 
AATCCTTGTA CCCCAAATAA CGTTGAGGTT CTGGAGACTC GATCAGCAGT TCCGAAAATA 
GAAGACACAG AAATAATCTC TCCCATAGCT GAGCGTCCGG AAGAGAGCTC TGATGCGGAA 
GCTGAGAACC ACGTGGTGGT GTCCTACTGC CCCCCTGTCA TTGAGGAGGA AACGCCGAAC 
CCCGGCGCAG ATGAGGCGGG GGGAGCCTCT CAAGTCGTTT ACATTGACAT CCAGTCTATG 
TACCAGCCTC AAGCGAAACC AGAGGAAGAA CAGGAAAATG ACCCTGTCGG GGGGGCCGGC 
TACAAGCCAC AGATGCACCT CCCCGTCACT TCTACTGTAG AAGACCTCGC TGCAGAGGAC 
GACCTAGATA AAGCTGCGGG TTACAGACCT CAGGCAAATG TCAACACTTG GAACTTAGTA 
TCTCCAGACT CCCCCAGGTC CACGGACAGC AACAGTGAAA TTGTCTCCTT TGGAAGCCCC 
TGCTCCATTA ACTCCCGACA GTTTCTGATT CCTCCTAAAG ATGAAGACTC TCCGAAATCC 
AGTGGGGGAG GGTGGTCCTT TACAAACTTC TTTCAGAACA AACCAAATGA CTAA