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CARS cDNA ORF clone, Canis lupus familiaris(Dog)

Summary

Gene Symbol CARS
Entrez Gene ID 475998
Full Name cysteinyl-tRNA synthetase
General protein information
Preferred Names

cysteinyl-tRNA synthetase

Names

cysteinyl-tRNA synthetase, cytoplasmic
cysteine--tRNA ligase, cytoplasmic
Gene Type protein-coding
Organism Canis lupus familiaris(Dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005631355.2 XP_005631412.1 cysteine--tRNA ligase, cytoplasmic isoform X2
XM_846131.4 XP_851224.1 cysteine--tRNA ligase, cytoplasmic isoform X1
XM_846131.3 XP_851224.1 cysteine--tRNA ligase, cytoplasmic isoform X1
XM_005631355.1 XP_005631412.1 cysteine--tRNA ligase, cytoplasmic isoform X2

Pathways from BioSystems


Homologies


CARS XP_001096302.1
Oryza sativa (rice) Os09g0556500 NP_001063899.1
Homo sapiens (human) CARS NP_001014437.1
Rattus norvegicus (Norway rat) Cars NP_001099789.1
Drosophila melanogaster (fruit fly) Aats-cys NP_611087.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012283 XP_320253.2
Arabidopsis thaliana (thale cress) SYCO ARATH NP_565717.2
Mus musculus (house mouse) Cars NP_038770.3
Gallus gallus (chicken) CARS NP_001012601.1
Caenorhabditis elegans (roundworm) cars-1 NP_491014.1
Saccharomyces cerevisiae (baker's yeast) YNL247W NP_014152.1
Kluyveromyces lactis KLLA0C03025g XP_452332.1
Pan troglodytes (chimpanzee) CARS XP_003312912.1
Canis lupus familiaris (dog) CARS XP_851224.1
Bos taurus (cattle) CARS NP_001096719.1
Xenopus tropicalis (tropical clawed frog) cars NP_001011365.1
Danio rerio (zebrafish) cars NP_001112372.1
Schizosaccharomyces pombe (fission yeast) SPAC29E6.06c NP_594564.1

Gene Ontology

The following CARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe20179 XM_846131.4
Latest version!		 Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OCe20180 XM_005631355.2
Latest version!		 Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OCe20179 XM_846131.3 Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OCe20180 XM_005631355.1 Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe20179
Clone ID Related Accession (Same CDS sequence) XM_846131.4 , XM_846131.3
Accession Version XM_846131.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product cysteine--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_846131.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTCAGGTT CCTCTGGGGA GCAGGCTCCT GCCTACCGGT CCATCCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCGGGGG 
TACTCCCTGT CCCAGGCAGA TGTGGATGCG TTCAGGCAGC TCTCCGCCCC TCCCGCTGAC 
ACGCGCCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCCATCCT GGGCGGCCCC 
TGCGCCAGAG GCGAGCCCTG TGGGCGCCAA GCAAGTAAGA GCCGGCGGGG GCAGCCTCAA 
TGGTCCCCTC CGGCTGGGTC TCCTCCGTGC AAGCTCCGCC TTTACAACAG CCTCACCCGG 
AACAAGGACG TGTTTATACC TCAGCATGGG AAGAAGGTGA CGTGGTATTG CTGTGGGCCT 
ACTGTGTACG ATGCGTCTCA CATGGGGCAT GCCAGGTCGT ACATCTCCTT CGACATCCTG 
AGGAGGGTGC TGCGTGACTA CTTCGGGTAT GATGTCTTCT ACTGCATGAA CATCACAGAT 
GTGGACGACA AGATCATCAA GAGGGCCCGG CAGAACTACT TGTTTGAGGA GTATCGGGAG 
AAGCAGCCAC GGGCGGCCCA GCTCCTGGAG GATGTTCACG CCGCCCTGAA GCTCTTCTCA 
GTGAAGTTAC AGGAGACCAC GGACCCCGAC AAAAAGCACA TGCTGGAGCG TATTCAGCAC 
ACAGTGCAGC TGGCTGCAGA GCCTCTGGAG AGAGCCGTGC AGAATGACCT CCCCGCAGAA 
GTGGCCGACA GCTGTGCAGA GGTGCTGTTG GAAGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGACTCCA CACTCGGCAG TGAGGTCACT GACAATTCTA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AGGAATTCCA CAAAGACATG GAAGCCCTGA ACGTTCTCCC TCCAGACGTC 
TTAACCCGCG TTAGTGAATA TGTGCCAGAG ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGCTACG GCTATGCTTC AAATGGGTCC GTCTACTTCG ATACGGTGAA GTTTGCTTCC 
AGCGAGAGAC ACTCCTACGG GAAGCTGGTG CCCGAGGCTG TTGGAGACCA GAAAGCCCTG 
CAGGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGGTCTCCT 
AACGACTTCG CCCTGTGGAA AGCCTCCAAG CCGGGCGAGC CGTCCTGGCC GTGCCCGTGG 
GGAAAGGGGC GTCCGGGATG GCACATCGAA TGCTCTGCCA TGGCGGGCAC TCTCCTGGGA 
GCCTCGATGG ACATTCATGG CGGAGGCTTT GACCTCCGGT TTCCCCACCA TGACAACGAG 
CTGGCTCAGT CGGAGGCCTA CTTCGAGAAT GATTGCTGGG TCCGCTACTT CCTCCACACG 
GGCCACCTGA CCATCGCTGG CTGCAAGATG TCCAAGTCAC TCAAAAACTT CATCACCATC 
AAAGACGCCT TGAAGAAGCA CTCAGCGCGG CAGCTGCGGC TGGCCTTCCT CATGCACTCC 
TGGAAGGACA CCCTGGATTA CTCGAGCAAC ACTATGGAGT CTGCACTGCA GTACGAGAAG 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACCTCCTCC GAGCTCCCGT TGATATTAGT 
GGTCGGTTTG AAAAGTGGGA AGATGAAGAA TCGGAACTGA ACAAGAGCTT TTACGACAAG 
AAGGCAGCGG TTCATGAAGC CCTCTGTGAC AACGTGGACA CACGCACTGT CTTGGAGGAA 
ATGAGGGCTT TGGTCAGCCA GTGCAACCTA TACATGGCGA CCCGGAAGGC TGCCAGGAGG 
AGGCCCAACC GGGCTCTGTT GGAAAGCATC GCTCTGTACC TCACCCGCAT GCTAAAGATC 
TTTGGGGCCA TTGAAGAGGA AAGCTCCCTG GGATTTCCAG TGCGAGGACC TGGAACCAGC 
CCAGATCTGG AATCCACGGT CATGCCGTAC CTGCAGGTGC TCTCCGAGTT CAGAGAAGGT 
GTGCGAAAGA TCGCCCGAGA GCAAAAAGTC CCTGAGGTTC TGCAGCTCAG TGATGCTCTG 
CGGGATGACA TCTTGCCCGA GCTTGGGGTG CGGCTTGAAG ATCACGAAGG GCTGCCAACT 
GTGGTGAAGC TGGGAGACAG AGAGGCCCTG CTGAGGGAGA GAGAGGAGAA GAGACGAGTT 
GAAGAGGAGA AGAGGAAGAA GAAGGAGGAG GCCGCCAGGA TAAAACAGGA GCAAGAGGCA 
GCGAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTGTCAGA AAGCGACAAA 
TACTCCAAGT TTGATGAAAA CGGTCTGCCC ACACACGACG TGGATGGCAA AGAGCTGAGC 
AAAGGGCAAG CCAAGAAGGT GAAGAAGCTC TATGAAGCTC AGGAGAAGCG CCACAAGGAG 
TATTTGCAAA TGGCTCAAAA CGGAAGCCTC AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_851224.1
CDS96..2591
Translation

Target ORF information:

RefSeq Version XM_846131.4
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_846131.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTCAGGTT CCTCTGGGGA GCAGGCTCCT GCCTACCGGT CCATCCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCGGGGG 
TACTCCCTGT CCCAGGCAGA TGTGGATGCG TTCAGGCAGC TCTCCGCCCC TCCCGCTGAC 
ACGCGCCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCCATCCT GGGCGGCCCC 
TGCGCCAGAG GCGAGCCCTG TGGGCGCCAA GCAAGTAAGA GCCGGCGGGG GCAGCCTCAA 
TGGTCCCCTC CGGCTGGGTC TCCTCCGTGC AAGCTCCGCC TTTACAACAG CCTCACCCGG 
AACAAGGACG TGTTTATACC TCAGCATGGG AAGAAGGTGA CGTGGTATTG CTGTGGGCCT 
ACTGTGTACG ATGCGTCTCA CATGGGGCAT GCCAGGTCGT ACATCTCCTT CGACATCCTG 
AGGAGGGTGC TGCGTGACTA CTTCGGGTAT GATGTCTTCT ACTGCATGAA CATCACAGAT 
GTGGACGACA AGATCATCAA GAGGGCCCGG CAGAACTACT TGTTTGAGGA GTATCGGGAG 
AAGCAGCCAC GGGCGGCCCA GCTCCTGGAG GATGTTCACG CCGCCCTGAA GCTCTTCTCA 
GTGAAGTTAC AGGAGACCAC GGACCCCGAC AAAAAGCACA TGCTGGAGCG TATTCAGCAC 
ACAGTGCAGC TGGCTGCAGA GCCTCTGGAG AGAGCCGTGC AGAATGACCT CCCCGCAGAA 
GTGGCCGACA GCTGTGCAGA GGTGCTGTTG GAAGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGACTCCA CACTCGGCAG TGAGGTCACT GACAATTCTA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AGGAATTCCA CAAAGACATG GAAGCCCTGA ACGTTCTCCC TCCAGACGTC 
TTAACCCGCG TTAGTGAATA TGTGCCAGAG ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGCTACG GCTATGCTTC AAATGGGTCC GTCTACTTCG ATACGGTGAA GTTTGCTTCC 
AGCGAGAGAC ACTCCTACGG GAAGCTGGTG CCCGAGGCTG TTGGAGACCA GAAAGCCCTG 
CAGGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGGTCTCCT 
AACGACTTCG CCCTGTGGAA AGCCTCCAAG CCGGGCGAGC CGTCCTGGCC GTGCCCGTGG 
GGAAAGGGGC GTCCGGGATG GCACATCGAA TGCTCTGCCA TGGCGGGCAC TCTCCTGGGA 
GCCTCGATGG ACATTCATGG CGGAGGCTTT GACCTCCGGT TTCCCCACCA TGACAACGAG 
CTGGCTCAGT CGGAGGCCTA CTTCGAGAAT GATTGCTGGG TCCGCTACTT CCTCCACACG 
GGCCACCTGA CCATCGCTGG CTGCAAGATG TCCAAGTCAC TCAAAAACTT CATCACCATC 
AAAGACGCCT TGAAGAAGCA CTCAGCGCGG CAGCTGCGGC TGGCCTTCCT CATGCACTCC 
TGGAAGGACA CCCTGGATTA CTCGAGCAAC ACTATGGAGT CTGCACTGCA GTACGAGAAG 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACCTCCTCC GAGCTCCCGT TGATATTAGT 
GGTCGGTTTG AAAAGTGGGA AGATGAAGAA TCGGAACTGA ACAAGAGCTT TTACGACAAG 
AAGGCAGCGG TTCATGAAGC CCTCTGTGAC AACGTGGACA CACGCACTGT CTTGGAGGAA 
ATGAGGGCTT TGGTCAGCCA GTGCAACCTA TACATGGCGA CCCGGAAGGC TGCCAGGAGG 
AGGCCCAACC GGGCTCTGTT GGAAAGCATC GCTCTGTACC TCACCCGCAT GCTAAAGATC 
TTTGGGGCCA TTGAAGAGGA AAGCTCCCTG GGATTTCCAG TGCGAGGACC TGGAACCAGC 
CCAGATCTGG AATCCACGGT CATGCCGTAC CTGCAGGTGC TCTCCGAGTT CAGAGAAGGT 
GTGCGAAAGA TCGCCCGAGA GCAAAAAGTC CCTGAGGTTC TGCAGCTCAG TGATGCTCTG 
CGGGATGACA TCTTGCCCGA GCTTGGGGTG CGGCTTGAAG ATCACGAAGG GCTGCCAACT 
GTGGTGAAGC TGGGAGACAG AGAGGCCCTG CTGAGGGAGA GAGAGGAGAA GAGACGAGTT 
GAAGAGGAGA AGAGGAAGAA GAAGGAGGAG GCCGCCAGGA TAAAACAGGA GCAAGAGGCA 
GCGAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTGTCAGA AAGCGACAAA 
TACTCCAAGT TTGATGAAAA CGGTCTGCCC ACACACGACG TGGATGGCAA AGAGCTGAGC 
AAAGGGCAAG CCAAGAAGGT GAAGAAGCTC TATGAAGCTC AGGAGAAGCG CCACAAGGAG 
TATTTGCAAA TGGCTCAAAA CGGAAGCCTC AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe20179
Clone ID Related Accession (Same CDS sequence) XM_846131.4 , XM_846131.3
Accession Version XM_846131.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product cysteine--tRNA ligase, cytoplasmic isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_846131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTCAGGTT CCTCTGGGGA GCAGGCTCCT GCCTACCGGT CCATCCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCGGGGG 
TACTCCCTGT CCCAGGCAGA TGTGGATGCG TTCAGGCAGC TCTCCGCCCC TCCCGCTGAC 
ACGCGCCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCCATCCT GGGCGGCCCC 
TGCGCCAGAG GCGAGCCCTG TGGGCGCCAA GCAAGTAAGA GCCGGCGGGG GCAGCCTCAA 
TGGTCCCCTC CGGCTGGGTC TCCTCCGTGC AAGCTCCGCC TTTACAACAG CCTCACCCGG 
AACAAGGACG TGTTTATACC TCAGCATGGG AAGAAGGTGA CGTGGTATTG CTGTGGGCCT 
ACTGTGTACG ATGCGTCTCA CATGGGGCAT GCCAGGTCGT ACATCTCCTT CGACATCCTG 
AGGAGGGTGC TGCGTGACTA CTTCGGGTAT GATGTCTTCT ACTGCATGAA CATCACAGAT 
GTGGACGACA AGATCATCAA GAGGGCCCGG CAGAACTACT TGTTTGAGGA GTATCGGGAG 
AAGCAGCCAC GGGCGGCCCA GCTCCTGGAG GATGTTCACG CCGCCCTGAA GCTCTTCTCA 
GTGAAGTTAC AGGAGACCAC GGACCCCGAC AAAAAGCACA TGCTGGAGCG TATTCAGCAC 
ACAGTGCAGC TGGCTGCAGA GCCTCTGGAG AGAGCCGTGC AGAATGACCT CCCCGCAGAA 
GTGGCCGACA GCTGTGCAGA GGTGCTGTTG GAAGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGACTCCA CACTCGGCAG TGAGGTCACT GACAATTCTA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AGGAATTCCA CAAAGACATG GAAGCCCTGA ACGTTCTCCC TCCAGACGTC 
TTAACCCGCG TTAGTGAATA TGTGCCAGAG ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGCTACG GCTATGCTTC AAATGGGTCC GTCTACTTCG ATACGGTGAA GTTTGCTTCC 
AGCGAGAGAC ACTCCTACGG GAAGCTGGTG CCCGAGGCTG TTGGAGACCA GAAAGCCCTG 
CAGGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGGTCTCCT 
AACGACTTCG CCCTGTGGAA AGCCTCCAAG CCGGGCGAGC CGTCCTGGCC GTGCCCGTGG 
GGAAAGGGGC GTCCGGGATG GCACATCGAA TGCTCTGCCA TGGCGGGCAC TCTCCTGGGA 
GCCTCGATGG ACATTCATGG CGGAGGCTTT GACCTCCGGT TTCCCCACCA TGACAACGAG 
CTGGCTCAGT CGGAGGCCTA CTTCGAGAAT GATTGCTGGG TCCGCTACTT CCTCCACACG 
GGCCACCTGA CCATCGCTGG CTGCAAGATG TCCAAGTCAC TCAAAAACTT CATCACCATC 
AAAGACGCCT TGAAGAAGCA CTCAGCGCGG CAGCTGCGGC TGGCCTTCCT CATGCACTCC 
TGGAAGGACA CCCTGGATTA CTCGAGCAAC ACTATGGAGT CTGCACTGCA GTACGAGAAG 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACCTCCTCC GAGCTCCCGT TGATATTAGT 
GGTCGGTTTG AAAAGTGGGA AGATGAAGAA TCGGAACTGA ACAAGAGCTT TTACGACAAG 
AAGGCAGCGG TTCATGAAGC CCTCTGTGAC AACGTGGACA CACGCACTGT CTTGGAGGAA 
ATGAGGGCTT TGGTCAGCCA GTGCAACCTA TACATGGCGA CCCGGAAGGC TGCCAGGAGG 
AGGCCCAACC GGGCTCTGTT GGAAAGCATC GCTCTGTACC TCACCCGCAT GCTAAAGATC 
TTTGGGGCCA TTGAAGAGGA AAGCTCCCTG GGATTTCCAG TGCGAGGACC TGGAACCAGC 
CCAGATCTGG AATCCACGGT CATGCCGTAC CTGCAGGTGC TCTCCGAGTT CAGAGAAGGT 
GTGCGAAAGA TCGCCCGAGA GCAAAAAGTC CCTGAGGTTC TGCAGCTCAG TGATGCTCTG 
CGGGATGACA TCTTGCCCGA GCTTGGGGTG CGGCTTGAAG ATCACGAAGG GCTGCCAACT 
GTGGTGAAGC TGGGAGACAG AGAGGCCCTG CTGAGGGAGA GAGAGGAGAA GAGACGAGTT 
GAAGAGGAGA AGAGGAAGAA GAAGGAGGAG GCCGCCAGGA TAAAACAGGA GCAAGAGGCA 
GCGAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTGTCAGA AAGCGACAAA 
TACTCCAAGT TTGATGAAAA CGGTCTGCCC ACACACGACG TGGATGGCAA AGAGCTGAGC 
AAAGGGCAAG CCAAGAAGGT GAAGAAGCTC TATGAAGCTC AGGAGAAGCG CCACAAGGAG 
TATTTGCAAA TGGCTCAAAA CGGAAGCCTC AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_851224.1
CDS70..2565
Translation

Target ORF information:

RefSeq Version XM_846131.3
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_846131.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTCAGGTT CCTCTGGGGA GCAGGCTCCT GCCTACCGGT CCATCCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCGGGGG 
TACTCCCTGT CCCAGGCAGA TGTGGATGCG TTCAGGCAGC TCTCCGCCCC TCCCGCTGAC 
ACGCGCCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCCATCCT GGGCGGCCCC 
TGCGCCAGAG GCGAGCCCTG TGGGCGCCAA GCAAGTAAGA GCCGGCGGGG GCAGCCTCAA 
TGGTCCCCTC CGGCTGGGTC TCCTCCGTGC AAGCTCCGCC TTTACAACAG CCTCACCCGG 
AACAAGGACG TGTTTATACC TCAGCATGGG AAGAAGGTGA CGTGGTATTG CTGTGGGCCT 
ACTGTGTACG ATGCGTCTCA CATGGGGCAT GCCAGGTCGT ACATCTCCTT CGACATCCTG 
AGGAGGGTGC TGCGTGACTA CTTCGGGTAT GATGTCTTCT ACTGCATGAA CATCACAGAT 
GTGGACGACA AGATCATCAA GAGGGCCCGG CAGAACTACT TGTTTGAGGA GTATCGGGAG 
AAGCAGCCAC GGGCGGCCCA GCTCCTGGAG GATGTTCACG CCGCCCTGAA GCTCTTCTCA 
GTGAAGTTAC AGGAGACCAC GGACCCCGAC AAAAAGCACA TGCTGGAGCG TATTCAGCAC 
ACAGTGCAGC TGGCTGCAGA GCCTCTGGAG AGAGCCGTGC AGAATGACCT CCCCGCAGAA 
GTGGCCGACA GCTGTGCAGA GGTGCTGTTG GAAGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGACTCCA CACTCGGCAG TGAGGTCACT GACAATTCTA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AGGAATTCCA CAAAGACATG GAAGCCCTGA ACGTTCTCCC TCCAGACGTC 
TTAACCCGCG TTAGTGAATA TGTGCCAGAG ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGCTACG GCTATGCTTC AAATGGGTCC GTCTACTTCG ATACGGTGAA GTTTGCTTCC 
AGCGAGAGAC ACTCCTACGG GAAGCTGGTG CCCGAGGCTG TTGGAGACCA GAAAGCCCTG 
CAGGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGGTCTCCT 
AACGACTTCG CCCTGTGGAA AGCCTCCAAG CCGGGCGAGC CGTCCTGGCC GTGCCCGTGG 
GGAAAGGGGC GTCCGGGATG GCACATCGAA TGCTCTGCCA TGGCGGGCAC TCTCCTGGGA 
GCCTCGATGG ACATTCATGG CGGAGGCTTT GACCTCCGGT TTCCCCACCA TGACAACGAG 
CTGGCTCAGT CGGAGGCCTA CTTCGAGAAT GATTGCTGGG TCCGCTACTT CCTCCACACG 
GGCCACCTGA CCATCGCTGG CTGCAAGATG TCCAAGTCAC TCAAAAACTT CATCACCATC 
AAAGACGCCT TGAAGAAGCA CTCAGCGCGG CAGCTGCGGC TGGCCTTCCT CATGCACTCC 
TGGAAGGACA CCCTGGATTA CTCGAGCAAC ACTATGGAGT CTGCACTGCA GTACGAGAAG 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACCTCCTCC GAGCTCCCGT TGATATTAGT 
GGTCGGTTTG AAAAGTGGGA AGATGAAGAA TCGGAACTGA ACAAGAGCTT TTACGACAAG 
AAGGCAGCGG TTCATGAAGC CCTCTGTGAC AACGTGGACA CACGCACTGT CTTGGAGGAA 
ATGAGGGCTT TGGTCAGCCA GTGCAACCTA TACATGGCGA CCCGGAAGGC TGCCAGGAGG 
AGGCCCAACC GGGCTCTGTT GGAAAGCATC GCTCTGTACC TCACCCGCAT GCTAAAGATC 
TTTGGGGCCA TTGAAGAGGA AAGCTCCCTG GGATTTCCAG TGCGAGGACC TGGAACCAGC 
CCAGATCTGG AATCCACGGT CATGCCGTAC CTGCAGGTGC TCTCCGAGTT CAGAGAAGGT 
GTGCGAAAGA TCGCCCGAGA GCAAAAAGTC CCTGAGGTTC TGCAGCTCAG TGATGCTCTG 
CGGGATGACA TCTTGCCCGA GCTTGGGGTG CGGCTTGAAG ATCACGAAGG GCTGCCAACT 
GTGGTGAAGC TGGGAGACAG AGAGGCCCTG CTGAGGGAGA GAGAGGAGAA GAGACGAGTT 
GAAGAGGAGA AGAGGAAGAA GAAGGAGGAG GCCGCCAGGA TAAAACAGGA GCAAGAGGCA 
GCGAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTGTCAGA AAGCGACAAA 
TACTCCAAGT TTGATGAAAA CGGTCTGCCC ACACACGACG TGGATGGCAA AGAGCTGAGC 
AAAGGGCAAG CCAAGAAGGT GAAGAAGCTC TATGAAGCTC AGGAGAAGCG CCACAAGGAG 
TATTTGCAAA TGGCTCAAAA CGGAAGCCTC AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe20180
Clone ID Related Accession (Same CDS sequence) XM_005631355.2 , XM_005631355.1
Accession Version XM_005631355.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product cysteine--tRNA ligase, cytoplasmic isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCAGGTT CCTCTGGGGA GCAGGGTAAG AGCCGGCGGG GGCAGCCTCA ATGGTCCCCT 
CCGGCTGGGT CTCCTCCGTG CAAGCTCCGC CTTTACAACA GCCTCACCCG GAACAAGGAC 
GTGTTTATAC CTCAGCATGG GAAGAAGGTG ACGTGGTATT GCTGTGGGCC TACTGTGTAC 
GATGCGTCTC ACATGGGGCA TGCCAGGTCG TACATCTCCT TCGACATCCT GAGGAGGGTG 
CTGCGTGACT ACTTCGGGTA TGATGTCTTC TACTGCATGA ACATCACAGA TGTGGACGAC 
AAGATCATCA AGAGGGCCCG GCAGAACTAC TTGTTTGAGG AGTATCGGGA GAAGCAGCCA 
CGGGCGGCCC AGCTCCTGGA GGATGTTCAC GCCGCCCTGA AGCTCTTCTC AGTGAAGTTA 
CAGGAGACCA CGGACCCCGA CAAAAAGCAC ATGCTGGAGC GTATTCAGCA CACAGTGCAG 
CTGGCTGCAG AGCCTCTGGA GAGAGCCGTG CAGAATGACC TCCCCGCAGA AGTGGCCGAC 
AGCTGTGCAG AGGTGCTGTT GGAAGAAGCA AAGGACTTGC TCTCTGACTG GCTGGACTCC 
ACACTCGGCA GTGAGGTCAC TGACAATTCT ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAGGAATTCC ACAAAGACAT GGAAGCCCTG AACGTTCTCC CTCCAGACGT CTTAACCCGC 
GTTAGTGAAT ATGTGCCAGA GATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGCTAC 
GGCTATGCTT CAAATGGGTC CGTCTACTTC GATACGGTGA AGTTTGCTTC CAGCGAGAGA 
CACTCCTACG GGAAGCTGGT GCCCGAGGCT GTTGGAGACC AGAAAGCCCT GCAGGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGGTCTCC TAACGACTTC 
GCCCTGTGGA AAGCCTCCAA GCCGGGCGAG CCGTCCTGGC CGTGCCCGTG GGGAAAGGGG 
CGTCCGGGAT GGCACATCGA ATGCTCTGCC ATGGCGGGCA CTCTCCTGGG AGCCTCGATG 
GACATTCATG GCGGAGGCTT TGACCTCCGG TTTCCCCACC ATGACAACGA GCTGGCTCAG 
TCGGAGGCCT ACTTCGAGAA TGATTGCTGG GTCCGCTACT TCCTCCACAC GGGCCACCTG 
ACCATCGCTG GCTGCAAGAT GTCCAAGTCA CTCAAAAACT TCATCACCAT CAAAGACGCC 
TTGAAGAAGC ACTCAGCGCG GCAGCTGCGG CTGGCCTTCC TCATGCACTC CTGGAAGGAC 
ACCCTGGATT ACTCGAGCAA CACTATGGAG TCTGCACTGC AGTACGAGAA GTTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACCTCCTC CGAGCTCCCG TTGATATTAG TGGTCGGTTT 
GAAAAGTGGG AAGATGAAGA ATCGGAACTG AACAAGAGCT TTTACGACAA GAAGGCAGCG 
GTTCATGAAG CCCTCTGTGA CAACGTGGAC ACACGCACTG TCTTGGAGGA AATGAGGGCT 
TTGGTCAGCC AGTGCAACCT ATACATGGCG ACCCGGAAGG CTGCCAGGAG GAGGCCCAAC 
CGGGCTCTGT TGGAAAGCAT CGCTCTGTAC CTCACCCGCA TGCTAAAGAT CTTTGGGGCC 
ATTGAAGAGG AAAGCTCCCT GGGATTTCCA GTGCGAGGAC CTGGAACCAG CCCAGATCTG 
GAATCCACGG TCATGCCGTA CCTGCAGGTG CTCTCCGAGT TCAGAGAAGG TGTGCGAAAG 
ATCGCCCGAG AGCAAAAAGT CCCTGAGGTT CTGCAGCTCA GTGATGCTCT GCGGGATGAC 
ATCTTGCCCG AGCTTGGGGT GCGGCTTGAA GATCACGAAG GGCTGCCAAC TGTGGTGAAG 
CTGGGAGACA GAGAGGCCCT GCTGAGGGAG AGAGAGGAGA AGAGACGAGT TGAAGAGGAG 
AAGAGGAAGA AGAAGGAGGA GGCCGCCAGG ATAAAACAGG AGCAAGAGGC AGCGAAGCTG 
GCCAAGATGA AGATCCCACC CAGTGAGATG TTCTTGTCAG AAAGCGACAA ATACTCCAAG 
TTTGATGAAA ACGGTCTGCC CACACACGAC GTGGATGGCA AAGAGCTGAG CAAAGGGCAA 
GCCAAGAAGG TGAAGAAGCT CTATGAAGCT CAGGAGAAGC GCCACAAGGA GTATTTGCAA 
ATGGCTCAAA ACGGAAGCCT CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005631412.1
CDS91..2337
Translation

Target ORF information:

RefSeq Version XM_005631355.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005631355.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCAGGTT CCTCTGGGGA GCAGGGTAAG AGCCGGCGGG GGCAGCCTCA ATGGTCCCCT 
CCGGCTGGGT CTCCTCCGTG CAAGCTCCGC CTTTACAACA GCCTCACCCG GAACAAGGAC 
GTGTTTATAC CTCAGCATGG GAAGAAGGTG ACGTGGTATT GCTGTGGGCC TACTGTGTAC 
GATGCGTCTC ACATGGGGCA TGCCAGGTCG TACATCTCCT TCGACATCCT GAGGAGGGTG 
CTGCGTGACT ACTTCGGGTA TGATGTCTTC TACTGCATGA ACATCACAGA TGTGGACGAC 
AAGATCATCA AGAGGGCCCG GCAGAACTAC TTGTTTGAGG AGTATCGGGA GAAGCAGCCA 
CGGGCGGCCC AGCTCCTGGA GGATGTTCAC GCCGCCCTGA AGCTCTTCTC AGTGAAGTTA 
CAGGAGACCA CGGACCCCGA CAAAAAGCAC ATGCTGGAGC GTATTCAGCA CACAGTGCAG 
CTGGCTGCAG AGCCTCTGGA GAGAGCCGTG CAGAATGACC TCCCCGCAGA AGTGGCCGAC 
AGCTGTGCAG AGGTGCTGTT GGAAGAAGCA AAGGACTTGC TCTCTGACTG GCTGGACTCC 
ACACTCGGCA GTGAGGTCAC TGACAATTCT ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAGGAATTCC ACAAAGACAT GGAAGCCCTG AACGTTCTCC CTCCAGACGT CTTAACCCGC 
GTTAGTGAAT ATGTGCCAGA GATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGCTAC 
GGCTATGCTT CAAATGGGTC CGTCTACTTC GATACGGTGA AGTTTGCTTC CAGCGAGAGA 
CACTCCTACG GGAAGCTGGT GCCCGAGGCT GTTGGAGACC AGAAAGCCCT GCAGGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGGTCTCC TAACGACTTC 
GCCCTGTGGA AAGCCTCCAA GCCGGGCGAG CCGTCCTGGC CGTGCCCGTG GGGAAAGGGG 
CGTCCGGGAT GGCACATCGA ATGCTCTGCC ATGGCGGGCA CTCTCCTGGG AGCCTCGATG 
GACATTCATG GCGGAGGCTT TGACCTCCGG TTTCCCCACC ATGACAACGA GCTGGCTCAG 
TCGGAGGCCT ACTTCGAGAA TGATTGCTGG GTCCGCTACT TCCTCCACAC GGGCCACCTG 
ACCATCGCTG GCTGCAAGAT GTCCAAGTCA CTCAAAAACT TCATCACCAT CAAAGACGCC 
TTGAAGAAGC ACTCAGCGCG GCAGCTGCGG CTGGCCTTCC TCATGCACTC CTGGAAGGAC 
ACCCTGGATT ACTCGAGCAA CACTATGGAG TCTGCACTGC AGTACGAGAA GTTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACCTCCTC CGAGCTCCCG TTGATATTAG TGGTCGGTTT 
GAAAAGTGGG AAGATGAAGA ATCGGAACTG AACAAGAGCT TTTACGACAA GAAGGCAGCG 
GTTCATGAAG CCCTCTGTGA CAACGTGGAC ACACGCACTG TCTTGGAGGA AATGAGGGCT 
TTGGTCAGCC AGTGCAACCT ATACATGGCG ACCCGGAAGG CTGCCAGGAG GAGGCCCAAC 
CGGGCTCTGT TGGAAAGCAT CGCTCTGTAC CTCACCCGCA TGCTAAAGAT CTTTGGGGCC 
ATTGAAGAGG AAAGCTCCCT GGGATTTCCA GTGCGAGGAC CTGGAACCAG CCCAGATCTG 
GAATCCACGG TCATGCCGTA CCTGCAGGTG CTCTCCGAGT TCAGAGAAGG TGTGCGAAAG 
ATCGCCCGAG AGCAAAAAGT CCCTGAGGTT CTGCAGCTCA GTGATGCTCT GCGGGATGAC 
ATCTTGCCCG AGCTTGGGGT GCGGCTTGAA GATCACGAAG GGCTGCCAAC TGTGGTGAAG 
CTGGGAGACA GAGAGGCCCT GCTGAGGGAG AGAGAGGAGA AGAGACGAGT TGAAGAGGAG 
AAGAGGAAGA AGAAGGAGGA GGCCGCCAGG ATAAAACAGG AGCAAGAGGC AGCGAAGCTG 
GCCAAGATGA AGATCCCACC CAGTGAGATG TTCTTGTCAG AAAGCGACAA ATACTCCAAG 
TTTGATGAAA ACGGTCTGCC CACACACGAC GTGGATGGCA AAGAGCTGAG CAAAGGGCAA 
GCCAAGAAGG TGAAGAAGCT CTATGAAGCT CAGGAGAAGC GCCACAAGGA GTATTTGCAA 
ATGGCTCAAA ACGGAAGCCT CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe20180
Clone ID Related Accession (Same CDS sequence) XM_005631355.2 , XM_005631355.1
Accession Version XM_005631355.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product cysteine--tRNA ligase, cytoplasmic isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCAGGTT CCTCTGGGGA GCAGGGTAAG AGCCGGCGGG GGCAGCCTCA ATGGTCCCCT 
CCGGCTGGGT CTCCTCCGTG CAAGCTCCGC CTTTACAACA GCCTCACCCG GAACAAGGAC 
GTGTTTATAC CTCAGCATGG GAAGAAGGTG ACGTGGTATT GCTGTGGGCC TACTGTGTAC 
GATGCGTCTC ACATGGGGCA TGCCAGGTCG TACATCTCCT TCGACATCCT GAGGAGGGTG 
CTGCGTGACT ACTTCGGGTA TGATGTCTTC TACTGCATGA ACATCACAGA TGTGGACGAC 
AAGATCATCA AGAGGGCCCG GCAGAACTAC TTGTTTGAGG AGTATCGGGA GAAGCAGCCA 
CGGGCGGCCC AGCTCCTGGA GGATGTTCAC GCCGCCCTGA AGCTCTTCTC AGTGAAGTTA 
CAGGAGACCA CGGACCCCGA CAAAAAGCAC ATGCTGGAGC GTATTCAGCA CACAGTGCAG 
CTGGCTGCAG AGCCTCTGGA GAGAGCCGTG CAGAATGACC TCCCCGCAGA AGTGGCCGAC 
AGCTGTGCAG AGGTGCTGTT GGAAGAAGCA AAGGACTTGC TCTCTGACTG GCTGGACTCC 
ACACTCGGCA GTGAGGTCAC TGACAATTCT ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAGGAATTCC ACAAAGACAT GGAAGCCCTG AACGTTCTCC CTCCAGACGT CTTAACCCGC 
GTTAGTGAAT ATGTGCCAGA GATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGCTAC 
GGCTATGCTT CAAATGGGTC CGTCTACTTC GATACGGTGA AGTTTGCTTC CAGCGAGAGA 
CACTCCTACG GGAAGCTGGT GCCCGAGGCT GTTGGAGACC AGAAAGCCCT GCAGGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGGTCTCC TAACGACTTC 
GCCCTGTGGA AAGCCTCCAA GCCGGGCGAG CCGTCCTGGC CGTGCCCGTG GGGAAAGGGG 
CGTCCGGGAT GGCACATCGA ATGCTCTGCC ATGGCGGGCA CTCTCCTGGG AGCCTCGATG 
GACATTCATG GCGGAGGCTT TGACCTCCGG TTTCCCCACC ATGACAACGA GCTGGCTCAG 
TCGGAGGCCT ACTTCGAGAA TGATTGCTGG GTCCGCTACT TCCTCCACAC GGGCCACCTG 
ACCATCGCTG GCTGCAAGAT GTCCAAGTCA CTCAAAAACT TCATCACCAT CAAAGACGCC 
TTGAAGAAGC ACTCAGCGCG GCAGCTGCGG CTGGCCTTCC TCATGCACTC CTGGAAGGAC 
ACCCTGGATT ACTCGAGCAA CACTATGGAG TCTGCACTGC AGTACGAGAA GTTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACCTCCTC CGAGCTCCCG TTGATATTAG TGGTCGGTTT 
GAAAAGTGGG AAGATGAAGA ATCGGAACTG AACAAGAGCT TTTACGACAA GAAGGCAGCG 
GTTCATGAAG CCCTCTGTGA CAACGTGGAC ACACGCACTG TCTTGGAGGA AATGAGGGCT 
TTGGTCAGCC AGTGCAACCT ATACATGGCG ACCCGGAAGG CTGCCAGGAG GAGGCCCAAC 
CGGGCTCTGT TGGAAAGCAT CGCTCTGTAC CTCACCCGCA TGCTAAAGAT CTTTGGGGCC 
ATTGAAGAGG AAAGCTCCCT GGGATTTCCA GTGCGAGGAC CTGGAACCAG CCCAGATCTG 
GAATCCACGG TCATGCCGTA CCTGCAGGTG CTCTCCGAGT TCAGAGAAGG TGTGCGAAAG 
ATCGCCCGAG AGCAAAAAGT CCCTGAGGTT CTGCAGCTCA GTGATGCTCT GCGGGATGAC 
ATCTTGCCCG AGCTTGGGGT GCGGCTTGAA GATCACGAAG GGCTGCCAAC TGTGGTGAAG 
CTGGGAGACA GAGAGGCCCT GCTGAGGGAG AGAGAGGAGA AGAGACGAGT TGAAGAGGAG 
AAGAGGAAGA AGAAGGAGGA GGCCGCCAGG ATAAAACAGG AGCAAGAGGC AGCGAAGCTG 
GCCAAGATGA AGATCCCACC CAGTGAGATG TTCTTGTCAG AAAGCGACAA ATACTCCAAG 
TTTGATGAAA ACGGTCTGCC CACACACGAC GTGGATGGCA AAGAGCTGAG CAAAGGGCAA 
GCCAAGAAGG TGAAGAAGCT CTATGAAGCT CAGGAGAAGC GCCACAAGGA GTATTTGCAA 
ATGGCTCAAA ACGGAAGCCT CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005631412.1
CDS70..2316
Translation

Target ORF information:

RefSeq Version XM_005631355.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris cysteinyl-tRNA synthetase (CARS), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005631355.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCAGGTT CCTCTGGGGA GCAGGGTAAG AGCCGGCGGG GGCAGCCTCA ATGGTCCCCT 
CCGGCTGGGT CTCCTCCGTG CAAGCTCCGC CTTTACAACA GCCTCACCCG GAACAAGGAC 
GTGTTTATAC CTCAGCATGG GAAGAAGGTG ACGTGGTATT GCTGTGGGCC TACTGTGTAC 
GATGCGTCTC ACATGGGGCA TGCCAGGTCG TACATCTCCT TCGACATCCT GAGGAGGGTG 
CTGCGTGACT ACTTCGGGTA TGATGTCTTC TACTGCATGA ACATCACAGA TGTGGACGAC 
AAGATCATCA AGAGGGCCCG GCAGAACTAC TTGTTTGAGG AGTATCGGGA GAAGCAGCCA 
CGGGCGGCCC AGCTCCTGGA GGATGTTCAC GCCGCCCTGA AGCTCTTCTC AGTGAAGTTA 
CAGGAGACCA CGGACCCCGA CAAAAAGCAC ATGCTGGAGC GTATTCAGCA CACAGTGCAG 
CTGGCTGCAG AGCCTCTGGA GAGAGCCGTG CAGAATGACC TCCCCGCAGA AGTGGCCGAC 
AGCTGTGCAG AGGTGCTGTT GGAAGAAGCA AAGGACTTGC TCTCTGACTG GCTGGACTCC 
ACACTCGGCA GTGAGGTCAC TGACAATTCT ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAGGAATTCC ACAAAGACAT GGAAGCCCTG AACGTTCTCC CTCCAGACGT CTTAACCCGC 
GTTAGTGAAT ATGTGCCAGA GATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGCTAC 
GGCTATGCTT CAAATGGGTC CGTCTACTTC GATACGGTGA AGTTTGCTTC CAGCGAGAGA 
CACTCCTACG GGAAGCTGGT GCCCGAGGCT GTTGGAGACC AGAAAGCCCT GCAGGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGGTCTCC TAACGACTTC 
GCCCTGTGGA AAGCCTCCAA GCCGGGCGAG CCGTCCTGGC CGTGCCCGTG GGGAAAGGGG 
CGTCCGGGAT GGCACATCGA ATGCTCTGCC ATGGCGGGCA CTCTCCTGGG AGCCTCGATG 
GACATTCATG GCGGAGGCTT TGACCTCCGG TTTCCCCACC ATGACAACGA GCTGGCTCAG 
TCGGAGGCCT ACTTCGAGAA TGATTGCTGG GTCCGCTACT TCCTCCACAC GGGCCACCTG 
ACCATCGCTG GCTGCAAGAT GTCCAAGTCA CTCAAAAACT TCATCACCAT CAAAGACGCC 
TTGAAGAAGC ACTCAGCGCG GCAGCTGCGG CTGGCCTTCC TCATGCACTC CTGGAAGGAC 
ACCCTGGATT ACTCGAGCAA CACTATGGAG TCTGCACTGC AGTACGAGAA GTTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACCTCCTC CGAGCTCCCG TTGATATTAG TGGTCGGTTT 
GAAAAGTGGG AAGATGAAGA ATCGGAACTG AACAAGAGCT TTTACGACAA GAAGGCAGCG 
GTTCATGAAG CCCTCTGTGA CAACGTGGAC ACACGCACTG TCTTGGAGGA AATGAGGGCT 
TTGGTCAGCC AGTGCAACCT ATACATGGCG ACCCGGAAGG CTGCCAGGAG GAGGCCCAAC 
CGGGCTCTGT TGGAAAGCAT CGCTCTGTAC CTCACCCGCA TGCTAAAGAT CTTTGGGGCC 
ATTGAAGAGG AAAGCTCCCT GGGATTTCCA GTGCGAGGAC CTGGAACCAG CCCAGATCTG 
GAATCCACGG TCATGCCGTA CCTGCAGGTG CTCTCCGAGT TCAGAGAAGG TGTGCGAAAG 
ATCGCCCGAG AGCAAAAAGT CCCTGAGGTT CTGCAGCTCA GTGATGCTCT GCGGGATGAC 
ATCTTGCCCG AGCTTGGGGT GCGGCTTGAA GATCACGAAG GGCTGCCAAC TGTGGTGAAG 
CTGGGAGACA GAGAGGCCCT GCTGAGGGAG AGAGAGGAGA AGAGACGAGT TGAAGAGGAG 
AAGAGGAAGA AGAAGGAGGA GGCCGCCAGG ATAAAACAGG AGCAAGAGGC AGCGAAGCTG 
GCCAAGATGA AGATCCCACC CAGTGAGATG TTCTTGTCAG AAAGCGACAA ATACTCCAAG 
TTTGATGAAA ACGGTCTGCC CACACACGAC GTGGATGGCA AAGAGCTGAG CAAAGGGCAA 
GCCAAGAAGG TGAAGAAGCT CTATGAAGCT CAGGAGAAGC GCCACAAGGA GTATTTGCAA 
ATGGCTCAAA ACGGAAGCCT CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed