WNK1 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following WNK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe282750 XM_022411013.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe27551 XM_003433482.4
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27552 XM_005637356.3
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27553 XM_022411000.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27554 XM_022411003.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27555 XM_022411004.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27556 XM_022411006.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe282743 XM_022411001.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe282744 XM_022411002.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe282745 XM_022411007.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe282746 XM_022411008.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe282747 XM_022411009.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe282748 XM_022411010.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe282749 XM_022411011.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43570 XM_014108325.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43571 XM_014108326.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43572 XM_014108327.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43573 XM_014108328.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43574 XM_014108329.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43575 XM_014108330.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43576 XM_014108331.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43577 XM_014108332.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43578 XM_014108333.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43579 XM_014108334.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43580 XM_014108335.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43581 XM_014108336.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43582 XM_014108338.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43583 XM_014108339.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43584 XM_014108340.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43585 XM_014108341.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43586 XM_014108342.1
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43587 XM_014108343.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43588 XM_014108344.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43589 XM_014108345.2
Latest version!
Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OCe43581 XM_014108336.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43579 XM_014108334.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe27551 XM_003433482.3 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43585 XM_014108341.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43587 XM_014108343.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe27552 XM_005637356.2 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43573 XM_014108328.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43588 XM_014108344.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe43582 XM_014108338.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe43589 XM_014108345.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe282750
    Clone ID Related Accession (Same CDS sequence) XM_022411013.1
    Accession Version XM_022411013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATTTTA CAAAGAAAGA CTTCTGCTCA GTGTTTGTCA TCATAAATTC TCATTGCTGC 
    TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGAGTAAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAA GGCAAAACAA AGATGAAAGG
    TATTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AGTTATGGCT ACGTATTGAA
    GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC
    TTGGAAAGAG ATGTGCCGGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTACGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTATCATT AATTAAGAGA
    AAACGAGAAC AGCGGCAGTT GGTTCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGGGC
    AGTCTCAAAC AGCAGGTAGA ACAACAATCA AGTACTTCCC AGGCAGGAGT CAAGCAGATC
    CCTTCTGTCA GCACTGGCGT GCCTACTGCT TCTACCACTT CAGCTTCAGT TTCTACGCAA
    GTAGAACCTG AAGAACCTGA GGCAGATCAA CATCAACAGC TACAGTACCA ACAAACTAGT
    ATATCTGTAT TATCTGACGG GACTGTTGAC AGTGGTCAAG GATCTTCTGT CTTCACAGAA
    TCTCGAGTGA GCAGCCAACA GACAGTTTCA TATGGGTCCC AGCATGAACA GGCACATTCT
    ACTGGCACAC TCCCAGGGCA TACAGCTTCT GTTGTCCAGG CACAATCTCA GCCCCATGGG
    GTCTATCCAC CCTCAAGTGT GGGGCAAAGC CAGGGTCAGC CATCCTCAAG TAGCTTAACA
    GGGATTCCAT CTTCCCTCCC CATACAACAT TCTCAGCAGC AGCAGGGAGT ACAGCAGACA
    GCCCCTCCAC AACAGACAGC GCAGTATTCG CTTCCACAGA CATCAGCCTC CAGTGAGGCC
    ACTATTGCAC AACCAGTCAG TCAGTCTCAG GCTCCACAGG TCTTGCCTCA AATATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGGCAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT CCCTGTGGGA
    CAGCCACTCT CTACTTCCTT ACTCCCTCAG TACCCGGTCT CTCAAATTCC CATATCAACT
    CCTCATGCGT CTGCGCCCCA GACAGGTTTT TCATCCCTTC CCATTACAAT GGCAGCTGGC
    ATTAATCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CAGCTGCGAT CCCAGGGGGA
    CCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAACTCCT GCAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTCCCTCT GGAGATGTTC TGTACCAGGG CTTTCCATCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CTTCTTCCAG CGTGGCTTCT
    ATTTGCATCC CTTCTACAGT CCTATCCCCT CCCATGCCCA CAGAAGCACT GGCTACACCG
    GGTTACTTTC CTACAGTGGT GCAGCCTTTT GTGGAATCTA ACCTTTTGGT TCCTGTGGGC
    AGTATAGGAG GACAGGTTCA AGTGTCCCAG CCAGCAGTGA GTTTAGCACA ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGCTCTGGAG CAGAATGAAA TGCCAATGTG GGGGCCTTGT
    TGCCACTGGC TGTTCTGTTG GGAAAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG
    CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT
    TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG
    CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT
    GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA
    GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT
    GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG
    GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC
    AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG
    GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT
    GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT
    TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA
    GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC
    TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG
    CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT
    CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA
    CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT
    CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT
    GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT
    ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT
    CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT
    TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT
    GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT
    GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC
    TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT
    CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA
    GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT
    ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC
    CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG
    ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT
    CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA
    GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG
    GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT
    GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT
    TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT
    ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT
    GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA
    GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC
    CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT
    GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA
    GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT
    TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT
    CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT
    TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT
    AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT
    GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA
    ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG
    GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT
    CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA
    CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT
    CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC
    CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT
    GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC
    CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA
    AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT
    CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT
    TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA
    CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG
    AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA
    ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC
    ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC
    TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG
    TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC
    ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG
    CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA
    CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC
    AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266721.1
    CDS296..6412
    Translation

    Target ORF information:

    RefSeq Version XM_022411013.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATTTTA CAAAGAAAGA CTTCTGCTCA GTGTTTGTCA TCATAAATTC TCATTGCTGC 
    TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGAGTAAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAA GGCAAAACAA AGATGAAAGG
    TATTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AGTTATGGCT ACGTATTGAA
    GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC
    TTGGAAAGAG ATGTGCCGGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTACGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTATCATT AATTAAGAGA
    AAACGAGAAC AGCGGCAGTT GGTTCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGGGC
    AGTCTCAAAC AGCAGGTAGA ACAACAATCA AGTACTTCCC AGGCAGGAGT CAAGCAGATC
    CCTTCTGTCA GCACTGGCGT GCCTACTGCT TCTACCACTT CAGCTTCAGT TTCTACGCAA
    GTAGAACCTG AAGAACCTGA GGCAGATCAA CATCAACAGC TACAGTACCA ACAAACTAGT
    ATATCTGTAT TATCTGACGG GACTGTTGAC AGTGGTCAAG GATCTTCTGT CTTCACAGAA
    TCTCGAGTGA GCAGCCAACA GACAGTTTCA TATGGGTCCC AGCATGAACA GGCACATTCT
    ACTGGCACAC TCCCAGGGCA TACAGCTTCT GTTGTCCAGG CACAATCTCA GCCCCATGGG
    GTCTATCCAC CCTCAAGTGT GGGGCAAAGC CAGGGTCAGC CATCCTCAAG TAGCTTAACA
    GGGATTCCAT CTTCCCTCCC CATACAACAT TCTCAGCAGC AGCAGGGAGT ACAGCAGACA
    GCCCCTCCAC AACAGACAGC GCAGTATTCG CTTCCACAGA CATCAGCCTC CAGTGAGGCC
    ACTATTGCAC AACCAGTCAG TCAGTCTCAG GCTCCACAGG TCTTGCCTCA AATATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGGCAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT CCCTGTGGGA
    CAGCCACTCT CTACTTCCTT ACTCCCTCAG TACCCGGTCT CTCAAATTCC CATATCAACT
    CCTCATGCGT CTGCGCCCCA GACAGGTTTT TCATCCCTTC CCATTACAAT GGCAGCTGGC
    ATTAATCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CAGCTGCGAT CCCAGGGGGA
    CCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAACTCCT GCAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTCCCTCT GGAGATGTTC TGTACCAGGG CTTTCCATCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CTTCTTCCAG CGTGGCTTCT
    ATTTGCATCC CTTCTACAGT CCTATCCCCT CCCATGCCCA CAGAAGCACT GGCTACACCG
    GGTTACTTTC CTACAGTGGT GCAGCCTTTT GTGGAATCTA ACCTTTTGGT TCCTGTGGGC
    AGTATAGGAG GACAGGTTCA AGTGTCCCAG CCAGCAGTGA GTTTAGCACA ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGCTCTGGAG CAGAATGAAA TGCCAATGTG GGGGCCTTGT
    TGCCACTGGC TGTTCTGTTG GGAAAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG
    CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT
    TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG
    CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT
    GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA
    GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT
    GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG
    GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC
    AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG
    GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT
    GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT
    TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA
    GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC
    TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG
    CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT
    CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA
    CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT
    CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT
    GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT
    ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT
    CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT
    TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT
    GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT
    GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC
    TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT
    CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA
    GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT
    ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC
    CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG
    ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT
    CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA
    GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG
    GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT
    GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT
    TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT
    ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT
    GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA
    GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC
    CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT
    GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA
    GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT
    TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT
    CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT
    TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT
    AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT
    GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA
    ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG
    GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT
    CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA
    CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT
    CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC
    CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT
    GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC
    CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA
    AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT
    CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT
    TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA
    CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG
    AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA
    ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC
    ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC
    TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG
    TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC
    ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG
    CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA
    CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC
    AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27551
    Clone ID Related Accession (Same CDS sequence) XM_003433482.4 , XM_003433482.3
    Accession Version XM_003433482.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003433482.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT
    CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA
    CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG
    ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT
    GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA
    CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC
    CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT
    CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC
    TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT
    TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT
    ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT
    AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT
    TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG
    GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT
    GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT
    ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT
    TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT
    GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA
    CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT
    TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA
    AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA
    GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC
    TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT
    CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT
    GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT
    TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC
    TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC
    ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG
    TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT
    GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003433530.1
    CDS997..8937
    Translation

    Target ORF information:

    RefSeq Version XM_003433482.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003433482.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT
    CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA
    CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG
    ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT
    GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA
    CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC
    CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT
    CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC
    TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT
    TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT
    ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT
    AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT
    TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG
    GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT
    GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT
    ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT
    TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT
    GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA
    CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT
    TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA
    AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA
    GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC
    TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT
    CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT
    GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT
    TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC
    TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC
    ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG
    TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT
    GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27551
    Clone ID Related Accession (Same CDS sequence) XM_003433482.4 , XM_003433482.3
    Accession Version XM_003433482.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X22
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003433482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT
    CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA
    CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG
    ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT
    GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA
    CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC
    CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT
    CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC
    TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT
    TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT
    ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT
    AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT
    TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG
    GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT
    GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT
    ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT
    TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT
    GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA
    CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT
    TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA
    AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA
    GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC
    TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT
    CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT
    GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT
    TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC
    TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC
    ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG
    TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT
    GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003433530.1
    CDS99..8039
    Translation

    Target ORF information:

    RefSeq Version XM_003433482.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003433482.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT
    CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA
    CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG
    ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT
    GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA
    CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC
    CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT
    CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC
    TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT
    TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT
    ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT
    AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT
    TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG
    GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT
    GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT
    ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT
    TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT
    GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA
    CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT
    TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA
    AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA
    GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC
    TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT
    CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT
    GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT
    TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC
    TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC
    ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG
    TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT
    GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27552
    Clone ID Related Accession (Same CDS sequence) XM_005637356.3 , XM_005637356.2
    Accession Version XM_005637356.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA
    GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC
    ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC
    TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG
    CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637413.1
    CDS996..8348
    Translation

    Target ORF information:

    RefSeq Version XM_005637356.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637356.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA
    GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC
    ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC
    TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG
    CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27552
    Clone ID Related Accession (Same CDS sequence) XM_005637356.3 , XM_005637356.2
    Accession Version XM_005637356.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA
    GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC
    ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC
    TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG
    CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637413.1
    CDS99..7451
    Translation

    Target ORF information:

    RefSeq Version XM_005637356.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA
    GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC
    ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC
    TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG
    CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27553
    Clone ID Related Accession (Same CDS sequence) XM_022411000.1
    Accession Version XM_022411000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC
    ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT
    ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT
    GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT
    TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT
    CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA
    CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA
    AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA
    GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC
    CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA
    CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC
    AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA
    ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA
    CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA
    GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT
    TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC
    AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT
    ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT
    TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG
    TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT
    GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA
    ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA
    CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA
    GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC
    AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT
    GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA
    GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA
    TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA
    GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA
    GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA
    GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG
    TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT
    CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA
    AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA
    GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT
    AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA
    TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG
    CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC
    TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT
    CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG
    CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC
    CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA
    AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC
    ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA
    CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT
    GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC
    CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC
    AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT
    TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA
    AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT
    ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG
    TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG
    AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCCAGCT
    CAGCCTCCTG CCATGACATC CAGCAGGAAG GGCACATTCA CAGATGACCT ACACAAGTTG
    GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GAAGAGGAAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGA AAGTTCTCCG CACCTGGTCA GCTGTGCATC
    TCCATGACCT CAAATCTGGG TGGTTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA
    GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCAGC TCCCCCCTTT
    GGCACTCAGT GGAGTGGGAC GGGTGGCCCG GCACCACAGC CACTTGGCCA GTTCCAGCCT
    GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC
    CCCCCAGGTT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266708.1
    CDS996..8345
    Translation

    Target ORF information:

    RefSeq Version XM_022411000.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
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    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
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    4201
    4261
    4321
    4381
    4441
    4501
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
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    5581
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    5701
    5761
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    5881
    5941
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    6181
    6241
    6301
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    6421
    6481
    6541
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    6721
    6781
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    6901
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    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC
    ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT
    ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT
    GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT
    TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT
    CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA
    CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA
    AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA
    GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC
    CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA
    CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC
    AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA
    ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA
    CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA
    GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT
    TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC
    AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT
    ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT
    TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG
    TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT
    GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA
    ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA
    CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA
    GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC
    AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT
    GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA
    GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA
    TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA
    GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA
    GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA
    GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG
    TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT
    CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA
    AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA
    GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT
    AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA
    TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG
    CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC
    TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT
    CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG
    CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC
    CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA
    AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC
    ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA
    CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT
    GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC
    CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC
    AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT
    TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA
    AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT
    ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG
    TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG
    AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCCAGCT
    CAGCCTCCTG CCATGACATC CAGCAGGAAG GGCACATTCA CAGATGACCT ACACAAGTTG
    GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GAAGAGGAAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGA AAGTTCTCCG CACCTGGTCA GCTGTGCATC
    TCCATGACCT CAAATCTGGG TGGTTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA
    GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCAGC TCCCCCCTTT
    GGCACTCAGT GGAGTGGGAC GGGTGGCCCG GCACCACAGC CACTTGGCCA GTTCCAGCCT
    GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC
    CCCCCAGGTT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27554
    Clone ID Related Accession (Same CDS sequence) XM_022411003.1
    Accession Version XM_022411003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA
    TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC
    TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA
    GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT
    CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT
    CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC
    ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA
    GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT
    GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG
    GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG
    TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT
    TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA
    GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC
    CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT
    TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG
    CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG
    ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC
    CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT
    CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG
    TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT
    GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC
    TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266711.1
    CDS996..8270
    Translation

    Target ORF information:

    RefSeq Version XM_022411003.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA
    TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC
    TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA
    GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT
    CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT
    CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC
    ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA
    GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT
    GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG
    GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG
    TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT
    TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA
    GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC
    CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT
    TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG
    CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG
    ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC
    CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT
    CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG
    TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT
    GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC
    TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27555
    Clone ID Related Accession (Same CDS sequence) XM_022411004.1
    Accession Version XM_022411004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA
    GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG
    CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC
    GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT
    TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA
    ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT
    ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT
    GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG
    ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA
    ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC
    CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT
    TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA
    GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT
    TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA
    GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA
    ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA
    GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT
    ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG
    TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA
    AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG
    AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC
    AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC
    AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT
    GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT
    ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT
    TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT
    CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG
    CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA
    ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG
    CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT
    GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA
    TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG
    CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT
    TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG
    GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT
    ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG
    CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG
    CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT
    GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA
    GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA
    GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC
    TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG
    CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA
    GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG
    GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA
    GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT
    GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT
    GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT
    TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG
    GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT
    GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA
    CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA
    GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG
    GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT
    TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT
    GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG
    AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG
    TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT
    AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA
    CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA
    AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA
    AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG
    CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC
    CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT
    TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG
    CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266712.1
    CDS996..8267
    Translation

    Target ORF information:

    RefSeq Version XM_022411004.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA
    GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG
    CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC
    GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT
    TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA
    ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT
    ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT
    GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG
    ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA
    ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC
    CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT
    TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA
    GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT
    TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA
    GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA
    ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA
    GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT
    ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG
    TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA
    AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG
    AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC
    AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC
    AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT
    GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT
    ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT
    TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT
    CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG
    CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA
    ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG
    CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT
    GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA
    TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG
    CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT
    TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG
    GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT
    ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG
    CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG
    CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT
    GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA
    GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA
    GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC
    TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG
    CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA
    GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG
    GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA
    GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT
    GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT
    GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT
    TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG
    GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT
    GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA
    CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA
    GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG
    GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT
    TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT
    GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG
    AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG
    TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT
    AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA
    CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA
    AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA
    AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG
    CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC
    CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT
    TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG
    CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27556
    Clone ID Related Accession (Same CDS sequence) XM_022411006.1
    Accession Version XM_022411006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT
    CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA
    GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC
    CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA
    ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT
    GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG
    AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC
    CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT
    TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC
    AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA
    CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA
    CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT
    GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA
    GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG
    GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG
    CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT
    CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG
    ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC
    ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCCAGCTC AGCCTCCTGC CATGACATCC
    AGCAGGAAGG GCACATTCAC AGATGACCTA CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG AAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAATCTGGGT
    GGTTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTACGG TTTTCCAGCT CCCCCCTTTG GCACTCAGTG GAGTGGGACG
    GGTGGCCCGG CACCACAGCC ACTTGGCCAG TTCCAGCCTG TGGGAACTGC CTCCTTACAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266714.1
    CDS996..8264
    Translation

    Target ORF information:

    RefSeq Version XM_022411006.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT
    CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA
    GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC
    CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA
    ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT
    GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG
    AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC
    CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT
    TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC
    AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA
    CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA
    CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT
    GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA
    GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG
    GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG
    CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT
    CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG
    ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC
    ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCCAGCTC AGCCTCCTGC CATGACATCC
    AGCAGGAAGG GCACATTCAC AGATGACCTA CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG AAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAATCTGGGT
    GGTTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTACGG TTTTCCAGCT CCCCCCTTTG GCACTCAGTG GAGTGGGACG
    GGTGGCCCGG CACCACAGCC ACTTGGCCAG TTCCAGCCTG TGGGAACTGC CTCCTTACAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282743
    Clone ID Related Accession (Same CDS sequence) XM_022411001.1
    Accession Version XM_022411001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266709.1
    CDS996..8294
    Translation

    Target ORF information:

    RefSeq Version XM_022411001.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282744
    Clone ID Related Accession (Same CDS sequence) XM_022411002.1
    Accession Version XM_022411002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT
    GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC
    CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC
    AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG
    TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC
    TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC
    CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC
    CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266710.1
    CDS996..8291
    Translation

    Target ORF information:

    RefSeq Version XM_022411002.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA
    CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG
    AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA
    CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA
    TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT
    CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT
    CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA
    CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT
    TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA
    ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA
    GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC
    CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC
    ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA
    ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC
    ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC
    CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA
    CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC
    ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA
    GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA
    GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC
    ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA
    ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT
    CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT
    CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC
    TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA
    CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC
    TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG
    GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC
    ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT
    TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA
    GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG
    ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG
    CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT
    CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT
    GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG
    ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG
    TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA
    AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC
    ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA
    ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT
    CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA
    GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA
    GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT
    GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG
    AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG
    CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA
    GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT
    GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT
    GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC
    CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC
    AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG
    TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC
    TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC
    CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC
    CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282745
    Clone ID Related Accession (Same CDS sequence) XM_022411007.1
    Accession Version XM_022411007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGCAGGA CACAGACCCC TTCTTCCTTT
    CTCATCAAAC CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266715.1
    CDS998..7912
    Translation

    Target ORF information:

    RefSeq Version XM_022411007.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGCAGGA CACAGACCCC TTCTTCCTTT
    CTCATCAAAC CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282746
    Clone ID Related Accession (Same CDS sequence) XM_022411008.1
    Accession Version XM_022411008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
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    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA
    CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT
    TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT
    TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT
    ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC
    CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA
    GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA
    GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT
    GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA
    AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA
    GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT
    GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG
    AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT
    GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT
    GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG
    TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT
    TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG
    TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG
    ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA
    GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT
    TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA
    TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA
    ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG
    GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA
    GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA
    ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA
    CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC
    ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT
    GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA
    TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG
    GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA
    GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA
    CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT
    GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG
    GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC
    TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT
    ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG
    CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC
    CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT
    CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT
    GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG
    CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG
    GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA
    GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG
    GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA
    AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA
    GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT
    GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC
    TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT
    ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA
    AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA
    GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA
    TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA
    CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT
    AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA
    GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG
    CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT
    GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC
    AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT
    AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT
    GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC
    AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT
    GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT
    GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CAAACACTGT CGGGGGAACA
    GTGAACAGCC AAGCTGCCCC AGCTCAGCCT CCTGCCATGA CATCCAGCAG GAAGGGCACA
    TTCACAGATG ACCTACACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA
    GGCAGAAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGAAAGTTC
    TCCGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGTTC TGCCCCCATC
    TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG
    TACGGTTTTC CAGCTCCCCC CTTTGGCACT CAGTGGAGTG GGACGGGTGG CCCGGCACCA
    CAGCCACTTG GCCAGTTCCA GCCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC
    AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266716.1
    CDS996..7889
    Translation

    Target ORF information:

    RefSeq Version XM_022411008.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA
    CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT
    TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT
    TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT
    ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC
    CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA
    GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA
    GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT
    GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA
    AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA
    GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT
    GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG
    AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT
    GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT
    GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG
    TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT
    TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG
    TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG
    ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA
    GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT
    TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA
    TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA
    ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG
    GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA
    GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA
    ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA
    CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC
    ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT
    GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA
    TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG
    GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA
    GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA
    CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT
    GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG
    GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC
    TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT
    ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG
    CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC
    CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT
    CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT
    GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG
    CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG
    GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA
    GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG
    GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA
    AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA
    GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT
    GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC
    TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT
    ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA
    AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA
    GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA
    TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA
    CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT
    AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA
    GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG
    CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT
    GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC
    AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT
    AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT
    GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC
    AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT
    GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT
    GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CAAACACTGT CGGGGGAACA
    GTGAACAGCC AAGCTGCCCC AGCTCAGCCT CCTGCCATGA CATCCAGCAG GAAGGGCACA
    TTCACAGATG ACCTACACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA
    GGCAGAAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGAAAGTTC
    TCCGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGTTC TGCCCCCATC
    TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG
    TACGGTTTTC CAGCTCCCCC CTTTGGCACT CAGTGGAGTG GGACGGGTGG CCCGGCACCA
    CAGCCACTTG GCCAGTTCCA GCCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC
    AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282747
    Clone ID Related Accession (Same CDS sequence) XM_022411009.1
    Accession Version XM_022411009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGCAGGAC ACAGACCCCT TCTTCCTTTC TCATCAAACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266717.1
    CDS998..7891
    Translation

    Target ORF information:

    RefSeq Version XM_022411009.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGCAGGAC ACAGACCCCT TCTTCCTTTC TCATCAAACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282748
    Clone ID Related Accession (Same CDS sequence) XM_022411010.1
    Accession Version XM_022411010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC
    TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC
    TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT
    GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT
    GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT
    ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT
    GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA
    GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT
    ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC
    TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC
    AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC
    CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT
    GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT
    GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT
    GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA
    TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA
    ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC
    CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA
    GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT
    CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA
    CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA
    TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT
    CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT
    ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC
    AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC
    AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA
    TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC
    ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT
    ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG
    GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA
    TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG
    CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA
    GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT
    CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA
    GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT
    TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT
    GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT
    ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA
    GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA
    ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG
    GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA
    GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT
    GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT
    ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC
    AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA
    CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG
    AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG
    AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC
    GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA
    CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT
    GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA
    TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266718.1
    CDS995..7552
    Translation

    Target ORF information:

    RefSeq Version XM_022411010.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC
    TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC
    TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT
    GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT
    GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT
    ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT
    GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA
    GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT
    ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC
    TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC
    AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC
    CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT
    GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT
    GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT
    GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA
    TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA
    ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC
    CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA
    GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT
    CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA
    CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA
    TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT
    CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT
    ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC
    AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC
    AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA
    TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC
    ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT
    ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG
    GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA
    TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG
    CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA
    GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT
    CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA
    GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT
    TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT
    GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT
    ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA
    GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA
    ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG
    GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA
    GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT
    GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT
    ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC
    AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA
    CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG
    AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG
    AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC
    GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA
    CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT
    GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA
    TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282749
    Clone ID Related Accession (Same CDS sequence) XM_022411011.1
    Accession Version XM_022411011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG
    ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC
    CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT
    CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG
    TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT
    GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC
    TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266719.1
    CDS995..7549
    Translation

    Target ORF information:

    RefSeq Version XM_022411011.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG
    ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC
    CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT
    CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG
    TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT
    GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC
    TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43570
    Clone ID Related Accession (Same CDS sequence) XM_014108325.1
    Accession Version XM_014108325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7380bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGGACCA GCAGCACAAA CACTGTCGGG
    GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG
    GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT
    CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA
    AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC
    CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA
    CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG
    GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC
    ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963800.1
    CDS99..7478
    Translation

    Target ORF information:

    RefSeq Version XM_014108325.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG
    CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGGACCA GCAGCACAAA CACTGTCGGG
    GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG
    GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT
    CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA
    AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC
    CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA
    CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG
    GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC
    ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43571
    Clone ID Related Accession (Same CDS sequence) XM_014108326.1
    Accession Version XM_014108326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC
    ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT
    ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT
    GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT
    TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT
    CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA
    CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA
    AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA
    GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC
    CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA
    CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC
    AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA
    ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA
    CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA
    GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT
    TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC
    AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT
    ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT
    TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG
    TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT
    GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA
    ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA
    CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA
    GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC
    AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT
    GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA
    GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA
    TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA
    GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA
    GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA
    GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG
    TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT
    CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA
    AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA
    GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT
    AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA
    TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG
    CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC
    TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT
    CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG
    CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC
    CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA
    AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC
    ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA
    CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT
    GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC
    CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC
    AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT
    TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA
    AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT
    ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG
    TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG
    AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTATGTGCC CTCCAGCAGA GCCGAAATCA GGGACCAGCA GCACAAACAC TGTCGGGGGA
    ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC
    ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC
    TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG
    TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC
    ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG
    CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA
    CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC
    AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963801.1
    CDS99..7475
    Translation

    Target ORF information:

    RefSeq Version XM_014108326.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT
    TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT
    CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA
    GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA
    GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT
    CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT
    TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA
    ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA
    GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC
    TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT
    TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC
    CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT
    TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC
    CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG
    GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC
    ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT
    ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT
    GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT
    TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT
    CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA
    CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA
    AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA
    GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC
    CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA
    CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC
    AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA
    ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA
    CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA
    GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT
    TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC
    AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT
    ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT
    TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG
    TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT
    GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA
    ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA
    CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA
    GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC
    AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT
    GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA
    GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA
    TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA
    GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA
    GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA
    GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG
    TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT
    CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA
    AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA
    GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT
    AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA
    TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG
    CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC
    TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT
    CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG
    CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC
    CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA
    AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC
    ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA
    CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT
    GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC
    CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC
    AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT
    TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA
    AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT
    ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG
    TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG
    AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTATGTGCC CTCCAGCAGA GCCGAAATCA GGGACCAGCA GCACAAACAC TGTCGGGGGA
    ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC
    ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC
    TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG
    TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC
    ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG
    CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA
    CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC
    AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43572
    Clone ID Related Accession (Same CDS sequence) XM_014108327.1
    Accession Version XM_014108327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963802.1
    CDS99..7457
    Translation

    Target ORF information:

    RefSeq Version XM_014108327.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43573
    Clone ID Related Accession (Same CDS sequence) XM_014108328.2 , XM_014108328.1
    Accession Version XM_014108328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    541
    601
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    4141
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    4261
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    4381
    4441
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    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
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    5341
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    5521
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    5881
    5941
    6001
    6061
    6121
    6181
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    6301
    6361
    6421
    6481
    6541
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963803.1
    CDS996..8327
    Translation

    Target ORF information:

    RefSeq Version XM_014108328.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43573
    Clone ID Related Accession (Same CDS sequence) XM_014108328.2 , XM_014108328.1
    Accession Version XM_014108328.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963803.1
    CDS99..7430
    Translation

    Target ORF information:

    RefSeq Version XM_014108328.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG
    AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT
    CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA
    AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA
    TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG
    GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG
    ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43574
    Clone ID Related Accession (Same CDS sequence) XM_014108329.1
    Accession Version XM_014108329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCAGAG CCGAAATCAG GGACCAGCAG CACAAACACT
    GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CCAGCTCAGC CTCCTGCCAT GACATCCAGC
    AGGAAGGGCA CATTCACAGA TGACCTACAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGAAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGAAAGT TCTCCGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA TCTGGGTGGT
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTACGGTTT TCCAGCTCCC CCCTTTGGCA CTCAGTGGAG TGGGACGGGT
    GGCCCGGCAC CACAGCCACT TGGCCAGTTC CAGCCTGTGG GAACTGCCTC CTTACAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963804.1
    CDS99..7424
    Translation

    Target ORF information:

    RefSeq Version XM_014108329.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCAGAG CCGAAATCAG GGACCAGCAG CACAAACACT
    GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CCAGCTCAGC CTCCTGCCAT GACATCCAGC
    AGGAAGGGCA CATTCACAGA TGACCTACAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGAAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGAAAGT TCTCCGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA TCTGGGTGGT
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTACGGTTT TCCAGCTCCC CCCTTTGGCA CTCAGTGGAG TGGGACGGGT
    GGCCCGGCAC CACAGCCACT TGGCCAGTTC CAGCCTGTGG GAACTGCCTC CTTACAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43575
    Clone ID Related Accession (Same CDS sequence) XM_014108330.1
    Accession Version XM_014108330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA
    TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC
    TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA
    GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT
    CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT
    CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC
    ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA
    GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT
    GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG
    GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG
    TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT
    TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA
    GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC
    CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT
    TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG
    CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACA AACACTGTCG GGGGAACAGT GAACAGCCAA
    GCTGCCCCAG CTCAGCCTCC TGCCATGACA TCCAGCAGGA AGGGCACATT CACAGATGAC
    CTACACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGAAGAGGA
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GAAAGTTCTC CGCACCTGGT
    CAGCTGTGCA TCTCCATGAC CTCAAATCTG GGTGGTTCTG CCCCCATCTC TGCAGCATCA
    GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA CGGTTTTCCA
    GCTCCCCCCT TTGGCACTCA GTGGAGTGGG ACGGGTGGCC CGGCACCACA GCCACTTGGC
    CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA CAGAATTTCA ACATCAGCAA TTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963805.1
    CDS99..7400
    Translation

    Target ORF information:

    RefSeq Version XM_014108330.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA
    TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC
    TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA
    GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT
    CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT
    CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC
    ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA
    GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT
    GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG
    GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG
    TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT
    TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA
    GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC
    CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT
    TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG
    CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT
    CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC
    TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC
    CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA
    AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA
    AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA
    TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT
    CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT
    TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT
    CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC
    AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA
    GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA
    TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA
    GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT
    TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA
    GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC
    ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT
    CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC
    TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA
    TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT
    TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT
    TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT
    GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC
    CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT
    GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG
    CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA
    TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC
    ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA
    GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA
    GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA
    CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA
    ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT
    CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA
    GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT
    CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC
    CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT
    AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT
    GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT
    GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT
    TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG
    CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG
    GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC
    CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT
    CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA
    AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT
    AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT
    CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG
    CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACA AACACTGTCG GGGGAACAGT GAACAGCCAA
    GCTGCCCCAG CTCAGCCTCC TGCCATGACA TCCAGCAGGA AGGGCACATT CACAGATGAC
    CTACACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGAAGAGGA
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GAAAGTTCTC CGCACCTGGT
    CAGCTGTGCA TCTCCATGAC CTCAAATCTG GGTGGTTCTG CCCCCATCTC TGCAGCATCA
    GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA CGGTTTTCCA
    GCTCCCCCCT TTGGCACTCA GTGGAGTGGG ACGGGTGGCC CGGCACCACA GCCACTTGGC
    CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA CAGAATTTCA ACATCAGCAA TTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43576
    Clone ID Related Accession (Same CDS sequence) XM_014108331.1
    Accession Version XM_014108331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA
    GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG
    CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC
    GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT
    TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA
    ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT
    ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT
    GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG
    ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA
    ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC
    CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT
    TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA
    GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT
    TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA
    GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA
    ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA
    GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT
    ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG
    TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA
    AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG
    AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC
    AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC
    AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT
    GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT
    ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT
    TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT
    CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG
    CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA
    ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG
    CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT
    GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA
    TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG
    CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT
    TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG
    GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT
    ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG
    CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG
    CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT
    GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA
    GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA
    GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC
    TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG
    CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA
    GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG
    GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA
    GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT
    GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT
    GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT
    TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG
    GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT
    GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA
    CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA
    GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG
    GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT
    TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT
    GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG
    AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG
    TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT
    AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA
    CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA
    AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA
    AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG
    CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC
    CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT
    TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG
    CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963806.1
    CDS99..7397
    Translation

    Target ORF information:

    RefSeq Version XM_014108331.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG
    GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA
    GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG
    CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC
    GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT
    TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA
    ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT
    ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT
    GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG
    ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA
    ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC
    CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT
    TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA
    GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT
    TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA
    GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA
    ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA
    GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT
    ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG
    TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA
    AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG
    AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC
    AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC
    AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT
    GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT
    ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT
    TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT
    CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG
    CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA
    ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG
    CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT
    GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA
    TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG
    CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT
    TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG
    GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT
    ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG
    CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG
    CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT
    GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA
    GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA
    GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC
    TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG
    CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA
    GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG
    GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA
    GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT
    GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT
    GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT
    TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG
    GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT
    GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA
    CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA
    GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG
    GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT
    TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT
    GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG
    AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG
    TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT
    AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA
    CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA
    AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA
    AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG
    CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC
    CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT
    TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG
    CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA
    GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT
    GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA
    CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC
    AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG
    CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT
    ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT
    CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG
    TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43577
    Clone ID Related Accession (Same CDS sequence) XM_014108332.1
    Accession Version XM_014108332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT
    CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA
    GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC
    CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA
    ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT
    GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG
    AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC
    CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT
    TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC
    AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA
    CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA
    CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT
    GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA
    GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG
    GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG
    CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT
    CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG
    ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCAGAG
    CCGAAATCAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC
    CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC
    AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG
    TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC
    TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC
    CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC
    CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963807.1
    CDS99..7394
    Translation

    Target ORF information:

    RefSeq Version XM_014108332.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT
    CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA
    GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC
    CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA
    ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT
    GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG
    AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC
    CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT
    TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC
    AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA
    CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA
    CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT
    GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA
    GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG
    GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG
    CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT
    CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG
    ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCAGAG
    CCGAAATCAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC
    CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC
    AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG
    TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC
    TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC
    CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC
    CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43578
    Clone ID Related Accession (Same CDS sequence) XM_014108333.1
    Accession Version XM_014108333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1381
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    1501
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    6661
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG
    GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCCAGCTCAG
    CCTCCTGCCA TGACATCCAG CAGGAAGGGC ACATTCACAG ATGACCTACA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGAA GAGGAAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGAAAG TTCTCCGCAC CTGGTCAGCT GTGCATCTCC
    ATGACCTCAA ATCTGGGTGG TTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCAGCTCC CCCCTTTGGC
    ACTCAGTGGA GTGGGACGGG TGGCCCGGCA CCACAGCCAC TTGGCCAGTT CCAGCCTGTG
    GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963808.1
    CDS99..7385
    Translation

    Target ORF information:

    RefSeq Version XM_014108333.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA
    AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG
    CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT
    GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA
    CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT
    AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA
    GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT
    CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT
    ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT
    AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA
    GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT
    AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT
    GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT
    GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT
    ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT
    GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG
    GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC
    AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA
    GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA
    GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG
    CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT
    CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG
    GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA
    CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG
    CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT
    AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA
    GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG
    ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG
    TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC
    CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT
    AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT
    GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT
    TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG
    GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA
    GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT
    AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC
    TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT
    GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT
    TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG
    TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA
    AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC
    TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC
    TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA
    AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT
    GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC
    AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT
    GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG
    CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG
    GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCCAGCTCAG
    CCTCCTGCCA TGACATCCAG CAGGAAGGGC ACATTCACAG ATGACCTACA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGAA GAGGAAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGAAAG TTCTCCGCAC CTGGTCAGCT GTGCATCTCC
    ATGACCTCAA ATCTGGGTGG TTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCAGCTCC CCCCTTTGGC
    ACTCAGTGGA GTGGGACGGG TGGCCCGGCA CCACAGCCAC TTGGCCAGTT CCAGCCTGTG
    GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43579
    Clone ID Related Accession (Same CDS sequence) XM_014108334.1 , XM_014108334.2
    Accession Version XM_014108334.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963809.1
    CDS99..7376
    Translation

    Target ORF information:

    RefSeq Version XM_014108334.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43579
    Clone ID Related Accession (Same CDS sequence) XM_014108334.1 , XM_014108334.2
    Accession Version XM_014108334.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963809.1
    CDS996..8273
    Translation

    Target ORF information:

    RefSeq Version XM_014108334.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108334.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
    CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC
    GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC
    ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC
    CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG
    CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG
    CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC
    GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC
    GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA
    GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG
    GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC
    AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC
    TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC
    AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA
    TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT
    CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC
    TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG
    GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG
    ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA
    TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT
    GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT
    CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA
    CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT
    ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA
    GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT
    GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA
    GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA
    GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG
    CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA
    CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG
    TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG
    TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG
    CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT
    CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC
    CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA
    GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT
    CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA
    CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT
    CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA
    GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA
    TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG
    CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG
    TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT
    CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG
    GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA
    ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA
    AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA
    AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG
    ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG
    ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG
    GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG
    GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA
    GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG
    CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT
    ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA
    CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA
    TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC
    AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC
    ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT
    GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG
    CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT
    CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA
    GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG
    AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT
    GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA
    GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA
    AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT
    GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA
    TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT
    TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC
    CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA
    CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC
    TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT
    CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA
    CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG
    CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT
    GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG
    TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT
    TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT
    CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT
    GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG
    GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT
    AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT
    AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT
    GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA
    GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA
    GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA
    ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG
    AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG
    GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG
    CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG
    CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC
    CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG
    ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC
    ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG
    GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA
    AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC
    AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG
    AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC
    TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.