PIAS1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Gene Symbol	PIAS1
Entrez Gene ID	478350
Full Name	protein inhibitor of activated STAT 1
General protein information	Preferred Names protein inhibitor of activated STAT 1 Names E3 SUMO-protein ligase PIAS1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_535524.5	XP_535524.2	E3 SUMO-protein ligase PIAS1 isoform X2
XM_022412853.1	XP_022268561.1	E3 SUMO-protein ligase PIAS1 isoform X6
XM_022412852.1	XP_022268560.1	E3 SUMO-protein ligase PIAS1 isoform X3
XM_022412850.1	XP_022268558.1	E3 SUMO-protein ligase PIAS1 isoform X1
XM_005638536.3	XP_005638593.1	E3 SUMO-protein ligase PIAS1 isoform X4
XM_005638536.2	XP_005638593.1	E3 SUMO-protein ligase PIAS1 isoform X1
XM_022412851.1	XP_022268559.1	E3 SUMO-protein ligase PIAS1 isoform X2
XM_535524.6	XP_535524.2	E3 SUMO-protein ligase PIAS1 isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIAS1	NP_057250.1
Canis lupus familiaris (dog)	PIAS1	XP_535524.2
Bos taurus (cattle)	PIAS1	NP_001068864.1
Mus musculus (house mouse)	Pias1	NP_062637.2
Pan troglodytes (chimpanzee)	PIAS1	XP_523109.3
Drosophila melanogaster (fruit fly)	Su(var)2-10	NP_001188888.1
	PIAS1	XP_001082929.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005031	XP_313897.4
Rattus norvegicus (Norway rat)	Pias1	NP_001100299.2
Gallus gallus (chicken)	PIAS1	NP_001026627.1
Xenopus tropicalis (tropical clawed frog)	pias1	XP_002932990.1

Gene Ontology

The following PIAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe29040	XM_005638536.3 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe29041	XM_535524.6 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe283741	XM_022412850.1 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe283742	XM_022412851.1 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe283743	XM_022412852.1 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe283744	XM_022412853.1 Latest version!	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe29040	XM_005638536.2	Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe29041	XM_535524.5	Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIAS1 ( XM_005638536.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638593.1 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe29040
Clone ID Related Accession (Same CDS sequence)	XM_005638536.3 , XM_005638536.2
Accession Version	XM_005638536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTTA TATCCTGCTT GCATCTGGTG TTTGAGCAAA TGGTTATGAG CCTTAGAGTT 
TCTGAACTCC AAGTACTGTT GGGCTACGCT GGGAGAAACA AGCACGGACG CAAACACGAA 
CTTCTCACAA AAGCCCTGCA TTTGCTAAAG GCTGGCTGCA GTCCTGCTGT GCAAATGAAA 
ATTAAAGAAC TCTATAGGCG GAGGTTCCCC CAGAAAATCA TGACGCCCGC GGACTTGTCC 
ATCCCCAATG TCCATTCAAG TCCTATGCCA GCAACTTTGT CTCCATCTAC CATTCCACAG 
CTCACTTACG ATGGTCACCC TGCATCATCA CCGTTACTCC CTGTTTCTCT TCTGGGACCT 
AAACATGAAC TGGAACTCCC ACATCTTACA TCAGCTCTTC ACCCAGTCCA TCCGGATATA 
AAACTTCAAA AATTACCATT TTATGATTTA CTGGATGAAC TGATAAAACC CACCAGTCTA 
GCATCAGACA ACAGTCAGCG CTTTCGAGAA ACCTGTTTTG CATTTGCATT GACACCACAA 
CAAGTGCAGC AAATCAGTAG TTCCATGGAT ATTTCTGGGA CCAAATGTGA CTTCACAGTG 
CAGGTCCAGT TAAGGTTTTG TTTATCAGAA ACCAGTTGTC CACAAGAAGA TCATTTCCCA 
CCCAATCTTT GTGTGAAAGT GAATACAAAA CCTTGCAGCC TCCCGGGTTA TCTTCCACCT 
ACAAAAAATG GTGTGGAACC AAAGCGACCC AGCCGACCAA TTAATATCAC CTCACTTGTC 
CGATTGTCCA CAACAGTACC AAACACCATT GTTGTTTCTT GGACTGCAGA AATTGGAAGA 
AACTATTCCA TGGCAGTGTA TCTTGTAAAA CAGTTGTCCT CAACAGTTCT TCTTCAGAGG 
TTACGAGCAA AGGGAATAAG GAATCCGGAT CATTCTAGAG CTTTAATTAA AGAGAAATTG 
ACTGCGGATC CAGACAGTGA AATAGCTACA ACCAGCCTAA GGGTTTCTCT ACTATGTCCA 
CTTGGTAAAA TGCGGCTGAC AATTCCATGT CGGGCCCTGA CATGTTCTCA TCTGCAGTGT 
TTTGATGCAA CTCTTTATAT TCAGATGAAT GAGAAAAAAC CAACCTGGGT TTGTCCTGTC 
TGTGATAAGA AGGCTCCATA CGAACACCTT ATTATTGATG GCTTGTTTAT GGAAATCCTA 
AAGTACTGTA CAGACTGCGA TGAGATACAG TTTAAGGAAG ATGGCTCTTG GGCACCTATG 
AGATCAAAAA AGGAAGTACA GGAAGTCTCT GCCTCGTACA ATGGAGTTGA TGGATGCTTG 
AGCTCCACAT TGGAGCATCA GGTGGCTTCT CACCACCAGT CCTCAAATAA AAATAAGAAA 
GTAGAGGTGA TTGACTTAAC CATAGACAGT TCCTCTGATG AAGAGGAGGA AGAGCCTTCT 
GCCAAGAGGA CCTGTCCTTC CCTATCTCCC ACATCACCAC TAAATAATAA AGGCATATTA 
AGTCTTCCAC ATCAAGCATC TCCAGTGTCC CGCACCCCGA GCCTCCCTGC TGTAGACACA 
AGCTACATTA ATACATCCCT CATCCAAGAC TACAGGCATC CTTTCCACAT GACACCCATG 
CCTTATGACC TACAAGGATT AGATTTCTTT CCTTTCTTAT CAGGAGACAA TCAGCATTAT 
AACACCTCCC TCCTGGCTGC TGCTGCAGCA GCAGTGTCAG ATGATCAAGA CCTCTTACAC 
TCGTCTCGGT TTTTCCCGTA TACCTCCTCG CAGATGTTTC TCGATCAGTT AAGTGCAGGA 
GGCAGTACTT CTCTGCCAAC CACCAATGGA AGCAGTAGTG GCAGTAACAG CAGCCTCGTG 
TCCTCCAACA GCCTGCGGGA GAGCCATAGC CACACTGTCG CAAACAGGAG CAGCACGGAC 
ACGGCATCCA TCTTTGGCAT CATACCAGAC ATTATTTCAT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638593.1
CDS	105..2072
Translation	MLFISCLHLVFEQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_005638536.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638536.3

ORF Insert Sequence:

ATGTTATTTA TATCCTGCTT GCATCTGGTG TTTGAGCAAA TGGTTATGAG CCTTAGAGTT 
TCTGAACTCC AAGTACTGTT GGGCTACGCT GGGAGAAACA AGCACGGACG CAAACACGAA 
CTTCTCACAA AAGCCCTGCA TTTGCTAAAG GCTGGCTGCA GTCCTGCTGT GCAAATGAAA 
ATTAAAGAAC TCTATAGGCG GAGGTTCCCC CAGAAAATCA TGACGCCCGC GGACTTGTCC 
ATCCCCAATG TCCATTCAAG TCCTATGCCA GCAACTTTGT CTCCATCTAC CATTCCACAG 
CTCACTTACG ATGGTCACCC TGCATCATCA CCGTTACTCC CTGTTTCTCT TCTGGGACCT 
AAACATGAAC TGGAACTCCC ACATCTTACA TCAGCTCTTC ACCCAGTCCA TCCGGATATA 
AAACTTCAAA AATTACCATT TTATGATTTA CTGGATGAAC TGATAAAACC CACCAGTCTA 
GCATCAGACA ACAGTCAGCG CTTTCGAGAA ACCTGTTTTG CATTTGCATT GACACCACAA 
CAAGTGCAGC AAATCAGTAG TTCCATGGAT ATTTCTGGGA CCAAATGTGA CTTCACAGTG 
CAGGTCCAGT TAAGGTTTTG TTTATCAGAA ACCAGTTGTC CACAAGAAGA TCATTTCCCA 
CCCAATCTTT GTGTGAAAGT GAATACAAAA CCTTGCAGCC TCCCGGGTTA TCTTCCACCT 
ACAAAAAATG GTGTGGAACC AAAGCGACCC AGCCGACCAA TTAATATCAC CTCACTTGTC 
CGATTGTCCA CAACAGTACC AAACACCATT GTTGTTTCTT GGACTGCAGA AATTGGAAGA 
AACTATTCCA TGGCAGTGTA TCTTGTAAAA CAGTTGTCCT CAACAGTTCT TCTTCAGAGG 
TTACGAGCAA AGGGAATAAG GAATCCGGAT CATTCTAGAG CTTTAATTAA AGAGAAATTG 
ACTGCGGATC CAGACAGTGA AATAGCTACA ACCAGCCTAA GGGTTTCTCT ACTATGTCCA 
CTTGGTAAAA TGCGGCTGAC AATTCCATGT CGGGCCCTGA CATGTTCTCA TCTGCAGTGT 
TTTGATGCAA CTCTTTATAT TCAGATGAAT GAGAAAAAAC CAACCTGGGT TTGTCCTGTC 
TGTGATAAGA AGGCTCCATA CGAACACCTT ATTATTGATG GCTTGTTTAT GGAAATCCTA 
AAGTACTGTA CAGACTGCGA TGAGATACAG TTTAAGGAAG ATGGCTCTTG GGCACCTATG 
AGATCAAAAA AGGAAGTACA GGAAGTCTCT GCCTCGTACA ATGGAGTTGA TGGATGCTTG 
AGCTCCACAT TGGAGCATCA GGTGGCTTCT CACCACCAGT CCTCAAATAA AAATAAGAAA 
GTAGAGGTGA TTGACTTAAC CATAGACAGT TCCTCTGATG AAGAGGAGGA AGAGCCTTCT 
GCCAAGAGGA CCTGTCCTTC CCTATCTCCC ACATCACCAC TAAATAATAA AGGCATATTA 
AGTCTTCCAC ATCAAGCATC TCCAGTGTCC CGCACCCCGA GCCTCCCTGC TGTAGACACA 
AGCTACATTA ATACATCCCT CATCCAAGAC TACAGGCATC CTTTCCACAT GACACCCATG 
CCTTATGACC TACAAGGATT AGATTTCTTT CCTTTCTTAT CAGGAGACAA TCAGCATTAT 
AACACCTCCC TCCTGGCTGC TGCTGCAGCA GCAGTGTCAG ATGATCAAGA CCTCTTACAC 
TCGTCTCGGT TTTTCCCGTA TACCTCCTCG CAGATGTTTC TCGATCAGTT AAGTGCAGGA 
GGCAGTACTT CTCTGCCAAC CACCAATGGA AGCAGTAGTG GCAGTAACAG CAGCCTCGTG 
TCCTCCAACA GCCTGCGGGA GAGCCATAGC CACACTGTCG CAAACAGGAG CAGCACGGAC 
ACGGCATCCA TCTTTGGCAT CATACCAGAC ATTATTTCAT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_005638536.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638593.1 Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29040
Clone ID Related Accession (Same CDS sequence)	XM_005638536.3 , XM_005638536.2
Accession Version	XM_005638536.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTTA TATCCTGCTT GCATCTGGTG TTTGAGCAAA TGGTTATGAG CCTTAGAGTT 
TCTGAACTCC AAGTACTGTT GGGCTACGCT GGGAGAAACA AGCACGGACG CAAACACGAA 
CTTCTCACAA AAGCCCTGCA TTTGCTAAAG GCTGGCTGCA GTCCTGCTGT GCAAATGAAA 
ATTAAAGAAC TCTATAGGCG GAGGTTCCCC CAGAAAATCA TGACGCCCGC GGACTTGTCC 
ATCCCCAATG TCCATTCAAG TCCTATGCCA GCAACTTTGT CTCCATCTAC CATTCCACAG 
CTCACTTACG ATGGTCACCC TGCATCATCA CCGTTACTCC CTGTTTCTCT TCTGGGACCT 
AAACATGAAC TGGAACTCCC ACATCTTACA TCAGCTCTTC ACCCAGTCCA TCCGGATATA 
AAACTTCAAA AATTACCATT TTATGATTTA CTGGATGAAC TGATAAAACC CACCAGTCTA 
GCATCAGACA ACAGTCAGCG CTTTCGAGAA ACCTGTTTTG CATTTGCATT GACACCACAA 
CAAGTGCAGC AAATCAGTAG TTCCATGGAT ATTTCTGGGA CCAAATGTGA CTTCACAGTG 
CAGGTCCAGT TAAGGTTTTG TTTATCAGAA ACCAGTTGTC CACAAGAAGA TCATTTCCCA 
CCCAATCTTT GTGTGAAAGT GAATACAAAA CCTTGCAGCC TCCCGGGTTA TCTTCCACCT 
ACAAAAAATG GTGTGGAACC AAAGCGACCC AGCCGACCAA TTAATATCAC CTCACTTGTC 
CGATTGTCCA CAACAGTACC AAACACCATT GTTGTTTCTT GGACTGCAGA AATTGGAAGA 
AACTATTCCA TGGCAGTGTA TCTTGTAAAA CAGTTGTCCT CAACAGTTCT TCTTCAGAGG 
TTACGAGCAA AGGGAATAAG GAATCCGGAT CATTCTAGAG CTTTAATTAA AGAGAAATTG 
ACTGCGGATC CAGACAGTGA AATAGCTACA ACCAGCCTAA GGGTTTCTCT ACTATGTCCA 
CTTGGTAAAA TGCGGCTGAC AATTCCATGT CGGGCCCTGA CATGTTCTCA TCTGCAGTGT 
TTTGATGCAA CTCTTTATAT TCAGATGAAT GAGAAAAAAC CAACCTGGGT TTGTCCTGTC 
TGTGATAAGA AGGCTCCATA CGAACACCTT ATTATTGATG GCTTGTTTAT GGAAATCCTA 
AAGTACTGTA CAGACTGCGA TGAGATACAG TTTAAGGAAG ATGGCTCTTG GGCACCTATG 
AGATCAAAAA AGGAAGTACA GGAAGTCTCT GCCTCGTACA ATGGAGTTGA TGGATGCTTG 
AGCTCCACAT TGGAGCATCA GGTGGCTTCT CACCACCAGT CCTCAAATAA AAATAAGAAA 
GTAGAGGTGA TTGACTTAAC CATAGACAGT TCCTCTGATG AAGAGGAGGA AGAGCCTTCT 
GCCAAGAGGA CCTGTCCTTC CCTATCTCCC ACATCACCAC TAAATAATAA AGGCATATTA 
AGTCTTCCAC ATCAAGCATC TCCAGTGTCC CGCACCCCGA GCCTCCCTGC TGTAGACACA 
AGCTACATTA ATACATCCCT CATCCAAGAC TACAGGCATC CTTTCCACAT GACACCCATG 
CCTTATGACC TACAAGGATT AGATTTCTTT CCTTTCTTAT CAGGAGACAA TCAGCATTAT 
AACACCTCCC TCCTGGCTGC TGCTGCAGCA GCAGTGTCAG ATGATCAAGA CCTCTTACAC 
TCGTCTCGGT TTTTCCCGTA TACCTCCTCG CAGATGTTTC TCGATCAGTT AAGTGCAGGA 
GGCAGTACTT CTCTGCCAAC CACCAATGGA AGCAGTAGTG GCAGTAACAG CAGCCTCGTG 
TCCTCCAACA GCCTGCGGGA GAGCCATAGC CACACTGTCG CAAACAGGAG CAGCACGGAC 
ACGGCATCCA TCTTTGGCAT CATACCAGAC ATTATTTCAT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638593.1
CDS	46..2013
Translation	MLFISCLHLVFEQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_005638536.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638536.2

ORF Insert Sequence:

ATGTTATTTA TATCCTGCTT GCATCTGGTG TTTGAGCAAA TGGTTATGAG CCTTAGAGTT 
TCTGAACTCC AAGTACTGTT GGGCTACGCT GGGAGAAACA AGCACGGACG CAAACACGAA 
CTTCTCACAA AAGCCCTGCA TTTGCTAAAG GCTGGCTGCA GTCCTGCTGT GCAAATGAAA 
ATTAAAGAAC TCTATAGGCG GAGGTTCCCC CAGAAAATCA TGACGCCCGC GGACTTGTCC 
ATCCCCAATG TCCATTCAAG TCCTATGCCA GCAACTTTGT CTCCATCTAC CATTCCACAG 
CTCACTTACG ATGGTCACCC TGCATCATCA CCGTTACTCC CTGTTTCTCT TCTGGGACCT 
AAACATGAAC TGGAACTCCC ACATCTTACA TCAGCTCTTC ACCCAGTCCA TCCGGATATA 
AAACTTCAAA AATTACCATT TTATGATTTA CTGGATGAAC TGATAAAACC CACCAGTCTA 
GCATCAGACA ACAGTCAGCG CTTTCGAGAA ACCTGTTTTG CATTTGCATT GACACCACAA 
CAAGTGCAGC AAATCAGTAG TTCCATGGAT ATTTCTGGGA CCAAATGTGA CTTCACAGTG 
CAGGTCCAGT TAAGGTTTTG TTTATCAGAA ACCAGTTGTC CACAAGAAGA TCATTTCCCA 
CCCAATCTTT GTGTGAAAGT GAATACAAAA CCTTGCAGCC TCCCGGGTTA TCTTCCACCT 
ACAAAAAATG GTGTGGAACC AAAGCGACCC AGCCGACCAA TTAATATCAC CTCACTTGTC 
CGATTGTCCA CAACAGTACC AAACACCATT GTTGTTTCTT GGACTGCAGA AATTGGAAGA 
AACTATTCCA TGGCAGTGTA TCTTGTAAAA CAGTTGTCCT CAACAGTTCT TCTTCAGAGG 
TTACGAGCAA AGGGAATAAG GAATCCGGAT CATTCTAGAG CTTTAATTAA AGAGAAATTG 
ACTGCGGATC CAGACAGTGA AATAGCTACA ACCAGCCTAA GGGTTTCTCT ACTATGTCCA 
CTTGGTAAAA TGCGGCTGAC AATTCCATGT CGGGCCCTGA CATGTTCTCA TCTGCAGTGT 
TTTGATGCAA CTCTTTATAT TCAGATGAAT GAGAAAAAAC CAACCTGGGT TTGTCCTGTC 
TGTGATAAGA AGGCTCCATA CGAACACCTT ATTATTGATG GCTTGTTTAT GGAAATCCTA 
AAGTACTGTA CAGACTGCGA TGAGATACAG TTTAAGGAAG ATGGCTCTTG GGCACCTATG 
AGATCAAAAA AGGAAGTACA GGAAGTCTCT GCCTCGTACA ATGGAGTTGA TGGATGCTTG 
AGCTCCACAT TGGAGCATCA GGTGGCTTCT CACCACCAGT CCTCAAATAA AAATAAGAAA 
GTAGAGGTGA TTGACTTAAC CATAGACAGT TCCTCTGATG AAGAGGAGGA AGAGCCTTCT 
GCCAAGAGGA CCTGTCCTTC CCTATCTCCC ACATCACCAC TAAATAATAA AGGCATATTA 
AGTCTTCCAC ATCAAGCATC TCCAGTGTCC CGCACCCCGA GCCTCCCTGC TGTAGACACA 
AGCTACATTA ATACATCCCT CATCCAAGAC TACAGGCATC CTTTCCACAT GACACCCATG 
CCTTATGACC TACAAGGATT AGATTTCTTT CCTTTCTTAT CAGGAGACAA TCAGCATTAT 
AACACCTCCC TCCTGGCTGC TGCTGCAGCA GCAGTGTCAG ATGATCAAGA CCTCTTACAC 
TCGTCTCGGT TTTTCCCGTA TACCTCCTCG CAGATGTTTC TCGATCAGTT AAGTGCAGGA 
GGCAGTACTT CTCTGCCAAC CACCAATGGA AGCAGTAGTG GCAGTAACAG CAGCCTCGTG 
TCCTCCAACA GCCTGCGGGA GAGCCATAGC CACACTGTCG CAAACAGGAG CAGCACGGAC 
ACGGCATCCA TCTTTGGCAT CATACCAGAC ATTATTTCAT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_535524.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535524.2 Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29041
Clone ID Related Accession (Same CDS sequence)	XM_535524.5 , XM_535524.6
Accession Version	XM_535524.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535524.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACA GTGCGGAACT AAAGCAAATG GTTATGAGCC TTAGAGTTTC TGAACTCCAA 
GTACTGTTGG GCTACGCTGG GAGAAACAAG CACGGACGCA AACACGAACT TCTCACAAAA 
GCCCTGCATT TGCTAAAGGC TGGCTGCAGT CCTGCTGTGC AAATGAAAAT TAAAGAACTC 
TATAGGCGGA GGTTCCCCCA GAAAATCATG ACGCCCGCGG ACTTGTCCAT CCCCAATGTC 
CATTCAAGTC CTATGCCAGC AACTTTGTCT CCATCTACCA TTCCACAGCT CACTTACGAT 
GGTCACCCTG CATCATCACC GTTACTCCCT GTTTCTCTTC TGGGACCTAA ACATGAACTG 
GAACTCCCAC ATCTTACATC AGCTCTTCAC CCAGTCCATC CGGATATAAA ACTTCAAAAA 
TTACCATTTT ATGATTTACT GGATGAACTG ATAAAACCCA CCAGTCTAGC ATCAGACAAC 
AGTCAGCGCT TTCGAGAAAC CTGTTTTGCA TTTGCATTGA CACCACAACA AGTGCAGCAA 
ATCAGTAGTT CCATGGATAT TTCTGGGACC AAATGTGACT TCACAGTGCA GGTCCAGTTA 
AGGTTTTGTT TATCAGAAAC CAGTTGTCCA CAAGAAGATC ATTTCCCACC CAATCTTTGT 
GTGAAAGTGA ATACAAAACC TTGCAGCCTC CCGGGTTATC TTCCACCTAC AAAAAATGGT 
GTGGAACCAA AGCGACCCAG CCGACCAATT AATATCACCT CACTTGTCCG ATTGTCCACA 
ACAGTACCAA ACACCATTGT TGTTTCTTGG ACTGCAGAAA TTGGAAGAAA CTATTCCATG 
GCAGTGTATC TTGTAAAACA GTTGTCCTCA ACAGTTCTTC TTCAGAGGTT ACGAGCAAAG 
GGAATAAGGA ATCCGGATCA TTCTAGAGCT TTAATTAAAG AGAAATTGAC TGCGGATCCA 
GACAGTGAAA TAGCTACAAC CAGCCTAAGG GTTTCTCTAC TATGTCCACT TGGTAAAATG 
CGGCTGACAA TTCCATGTCG GGCCCTGACA TGTTCTCATC TGCAGTGTTT TGATGCAACT 
CTTTATATTC AGATGAATGA GAAAAAACCA ACCTGGGTTT GTCCTGTCTG TGATAAGAAG 
GCTCCATACG AACACCTTAT TATTGATGGC TTGTTTATGG AAATCCTAAA GTACTGTACA 
GACTGCGATG AGATACAGTT TAAGGAAGAT GGCTCTTGGG CACCTATGAG ATCAAAAAAG 
GAAGTACAGG AAGTCTCTGC CTCGTACAAT GGAGTTGATG GATGCTTGAG CTCCACATTG 
GAGCATCAGG TGGCTTCTCA CCACCAGTCC TCAAATAAAA ATAAGAAAGT AGAGGTGATT 
GACTTAACCA TAGACAGTTC CTCTGATGAA GAGGAGGAAG AGCCTTCTGC CAAGAGGACC 
TGTCCTTCCC TATCTCCCAC ATCACCACTA AATAATAAAG GCATATTAAG TCTTCCACAT 
CAAGCATCTC CAGTGTCCCG CACCCCGAGC CTCCCTGCTG TAGACACAAG CTACATTAAT 
ACATCCCTCA TCCAAGACTA CAGGCATCCT TTCCACATGA CACCCATGCC TTATGACCTA 
CAAGGATTAG ATTTCTTTCC TTTCTTATCA GGAGACAATC AGCATTATAA CACCTCCCTC 
CTGGCTGCTG CTGCAGCAGC AGTGTCAGAT GATCAAGACC TCTTACACTC GTCTCGGTTT 
TTCCCGTATA CCTCCTCGCA GATGTTTCTC GATCAGTTAA GTGCAGGAGG CAGTACTTCT 
CTGCCAACCA CCAATGGAAG CAGTAGTGGC AGTAACAGCA GCCTCGTGTC CTCCAACAGC 
CTGCGGGAGA GCCATAGCCA CACTGTCGCA AACAGGAGCA GCACGGACAC GGCATCCATC 
TTTGGCATCA TACCAGACAT TATTTCATTG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535524.2
CDS	52..2007
Translation	MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_535524.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT, 1 (PIAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535524.5

ORF Insert Sequence:

ATGGCGGACA GTGCGGAACT AAAGCAAATG GTTATGAGCC TTAGAGTTTC TGAACTCCAA 
GTACTGTTGG GCTACGCTGG GAGAAACAAG CACGGACGCA AACACGAACT TCTCACAAAA 
GCCCTGCATT TGCTAAAGGC TGGCTGCAGT CCTGCTGTGC AAATGAAAAT TAAAGAACTC 
TATAGGCGGA GGTTCCCCCA GAAAATCATG ACGCCCGCGG ACTTGTCCAT CCCCAATGTC 
CATTCAAGTC CTATGCCAGC AACTTTGTCT CCATCTACCA TTCCACAGCT CACTTACGAT 
GGTCACCCTG CATCATCACC GTTACTCCCT GTTTCTCTTC TGGGACCTAA ACATGAACTG 
GAACTCCCAC ATCTTACATC AGCTCTTCAC CCAGTCCATC CGGATATAAA ACTTCAAAAA 
TTACCATTTT ATGATTTACT GGATGAACTG ATAAAACCCA CCAGTCTAGC ATCAGACAAC 
AGTCAGCGCT TTCGAGAAAC CTGTTTTGCA TTTGCATTGA CACCACAACA AGTGCAGCAA 
ATCAGTAGTT CCATGGATAT TTCTGGGACC AAATGTGACT TCACAGTGCA GGTCCAGTTA 
AGGTTTTGTT TATCAGAAAC CAGTTGTCCA CAAGAAGATC ATTTCCCACC CAATCTTTGT 
GTGAAAGTGA ATACAAAACC TTGCAGCCTC CCGGGTTATC TTCCACCTAC AAAAAATGGT 
GTGGAACCAA AGCGACCCAG CCGACCAATT AATATCACCT CACTTGTCCG ATTGTCCACA 
ACAGTACCAA ACACCATTGT TGTTTCTTGG ACTGCAGAAA TTGGAAGAAA CTATTCCATG 
GCAGTGTATC TTGTAAAACA GTTGTCCTCA ACAGTTCTTC TTCAGAGGTT ACGAGCAAAG 
GGAATAAGGA ATCCGGATCA TTCTAGAGCT TTAATTAAAG AGAAATTGAC TGCGGATCCA 
GACAGTGAAA TAGCTACAAC CAGCCTAAGG GTTTCTCTAC TATGTCCACT TGGTAAAATG 
CGGCTGACAA TTCCATGTCG GGCCCTGACA TGTTCTCATC TGCAGTGTTT TGATGCAACT 
CTTTATATTC AGATGAATGA GAAAAAACCA ACCTGGGTTT GTCCTGTCTG TGATAAGAAG 
GCTCCATACG AACACCTTAT TATTGATGGC TTGTTTATGG AAATCCTAAA GTACTGTACA 
GACTGCGATG AGATACAGTT TAAGGAAGAT GGCTCTTGGG CACCTATGAG ATCAAAAAAG 
GAAGTACAGG AAGTCTCTGC CTCGTACAAT GGAGTTGATG GATGCTTGAG CTCCACATTG 
GAGCATCAGG TGGCTTCTCA CCACCAGTCC TCAAATAAAA ATAAGAAAGT AGAGGTGATT 
GACTTAACCA TAGACAGTTC CTCTGATGAA GAGGAGGAAG AGCCTTCTGC CAAGAGGACC 
TGTCCTTCCC TATCTCCCAC ATCACCACTA AATAATAAAG GCATATTAAG TCTTCCACAT 
CAAGCATCTC CAGTGTCCCG CACCCCGAGC CTCCCTGCTG TAGACACAAG CTACATTAAT 
ACATCCCTCA TCCAAGACTA CAGGCATCCT TTCCACATGA CACCCATGCC TTATGACCTA 
CAAGGATTAG ATTTCTTTCC TTTCTTATCA GGAGACAATC AGCATTATAA CACCTCCCTC 
CTGGCTGCTG CTGCAGCAGC AGTGTCAGAT GATCAAGACC TCTTACACTC GTCTCGGTTT 
TTCCCGTATA CCTCCTCGCA GATGTTTCTC GATCAGTTAA GTGCAGGAGG CAGTACTTCT 
CTGCCAACCA CCAATGGAAG CAGTAGTGGC AGTAACAGCA GCCTCGTGTC CTCCAACAGC 
CTGCGGGAGA GCCATAGCCA CACTGTCGCA AACAGGAGCA GCACGGACAC GGCATCCATC 
TTTGGCATCA TACCAGACAT TATTTCATTG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_535524.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535524.2 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe29041
Clone ID Related Accession (Same CDS sequence)	XM_535524.5 , XM_535524.6
Accession Version	XM_535524.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535524.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACA GTGCGGAACT AAAGCAAATG GTTATGAGCC TTAGAGTTTC TGAACTCCAA 
GTACTGTTGG GCTACGCTGG GAGAAACAAG CACGGACGCA AACACGAACT TCTCACAAAA 
GCCCTGCATT TGCTAAAGGC TGGCTGCAGT CCTGCTGTGC AAATGAAAAT TAAAGAACTC 
TATAGGCGGA GGTTCCCCCA GAAAATCATG ACGCCCGCGG ACTTGTCCAT CCCCAATGTC 
CATTCAAGTC CTATGCCAGC AACTTTGTCT CCATCTACCA TTCCACAGCT CACTTACGAT 
GGTCACCCTG CATCATCACC GTTACTCCCT GTTTCTCTTC TGGGACCTAA ACATGAACTG 
GAACTCCCAC ATCTTACATC AGCTCTTCAC CCAGTCCATC CGGATATAAA ACTTCAAAAA 
TTACCATTTT ATGATTTACT GGATGAACTG ATAAAACCCA CCAGTCTAGC ATCAGACAAC 
AGTCAGCGCT TTCGAGAAAC CTGTTTTGCA TTTGCATTGA CACCACAACA AGTGCAGCAA 
ATCAGTAGTT CCATGGATAT TTCTGGGACC AAATGTGACT TCACAGTGCA GGTCCAGTTA 
AGGTTTTGTT TATCAGAAAC CAGTTGTCCA CAAGAAGATC ATTTCCCACC CAATCTTTGT 
GTGAAAGTGA ATACAAAACC TTGCAGCCTC CCGGGTTATC TTCCACCTAC AAAAAATGGT 
GTGGAACCAA AGCGACCCAG CCGACCAATT AATATCACCT CACTTGTCCG ATTGTCCACA 
ACAGTACCAA ACACCATTGT TGTTTCTTGG ACTGCAGAAA TTGGAAGAAA CTATTCCATG 
GCAGTGTATC TTGTAAAACA GTTGTCCTCA ACAGTTCTTC TTCAGAGGTT ACGAGCAAAG 
GGAATAAGGA ATCCGGATCA TTCTAGAGCT TTAATTAAAG AGAAATTGAC TGCGGATCCA 
GACAGTGAAA TAGCTACAAC CAGCCTAAGG GTTTCTCTAC TATGTCCACT TGGTAAAATG 
CGGCTGACAA TTCCATGTCG GGCCCTGACA TGTTCTCATC TGCAGTGTTT TGATGCAACT 
CTTTATATTC AGATGAATGA GAAAAAACCA ACCTGGGTTT GTCCTGTCTG TGATAAGAAG 
GCTCCATACG AACACCTTAT TATTGATGGC TTGTTTATGG AAATCCTAAA GTACTGTACA 
GACTGCGATG AGATACAGTT TAAGGAAGAT GGCTCTTGGG CACCTATGAG ATCAAAAAAG 
GAAGTACAGG AAGTCTCTGC CTCGTACAAT GGAGTTGATG GATGCTTGAG CTCCACATTG 
GAGCATCAGG TGGCTTCTCA CCACCAGTCC TCAAATAAAA ATAAGAAAGT AGAGGTGATT 
GACTTAACCA TAGACAGTTC CTCTGATGAA GAGGAGGAAG AGCCTTCTGC CAAGAGGACC 
TGTCCTTCCC TATCTCCCAC ATCACCACTA AATAATAAAG GCATATTAAG TCTTCCACAT 
CAAGCATCTC CAGTGTCCCG CACCCCGAGC CTCCCTGCTG TAGACACAAG CTACATTAAT 
ACATCCCTCA TCCAAGACTA CAGGCATCCT TTCCACATGA CACCCATGCC TTATGACCTA 
CAAGGATTAG ATTTCTTTCC TTTCTTATCA GGAGACAATC AGCATTATAA CACCTCCCTC 
CTGGCTGCTG CTGCAGCAGC AGTGTCAGAT GATCAAGACC TCTTACACTC GTCTCGGTTT 
TTCCCGTATA CCTCCTCGCA GATGTTTCTC GATCAGTTAA GTGCAGGAGG CAGTACTTCT 
CTGCCAACCA CCAATGGAAG CAGTAGTGGC AGTAACAGCA GCCTCGTGTC CTCCAACAGC 
CTGCGGGAGA GCCATAGCCA CACTGTCGCA AACAGGAGCA GCACGGACAC GGCATCCATC 
TTTGGCATCA TACCAGACAT TATTTCATTG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535524.2
CDS	76..2031
Translation	MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_535524.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535524.6

ORF Insert Sequence:

ATGGCGGACA GTGCGGAACT AAAGCAAATG GTTATGAGCC TTAGAGTTTC TGAACTCCAA 
GTACTGTTGG GCTACGCTGG GAGAAACAAG CACGGACGCA AACACGAACT TCTCACAAAA 
GCCCTGCATT TGCTAAAGGC TGGCTGCAGT CCTGCTGTGC AAATGAAAAT TAAAGAACTC 
TATAGGCGGA GGTTCCCCCA GAAAATCATG ACGCCCGCGG ACTTGTCCAT CCCCAATGTC 
CATTCAAGTC CTATGCCAGC AACTTTGTCT CCATCTACCA TTCCACAGCT CACTTACGAT 
GGTCACCCTG CATCATCACC GTTACTCCCT GTTTCTCTTC TGGGACCTAA ACATGAACTG 
GAACTCCCAC ATCTTACATC AGCTCTTCAC CCAGTCCATC CGGATATAAA ACTTCAAAAA 
TTACCATTTT ATGATTTACT GGATGAACTG ATAAAACCCA CCAGTCTAGC ATCAGACAAC 
AGTCAGCGCT TTCGAGAAAC CTGTTTTGCA TTTGCATTGA CACCACAACA AGTGCAGCAA 
ATCAGTAGTT CCATGGATAT TTCTGGGACC AAATGTGACT TCACAGTGCA GGTCCAGTTA 
AGGTTTTGTT TATCAGAAAC CAGTTGTCCA CAAGAAGATC ATTTCCCACC CAATCTTTGT 
GTGAAAGTGA ATACAAAACC TTGCAGCCTC CCGGGTTATC TTCCACCTAC AAAAAATGGT 
GTGGAACCAA AGCGACCCAG CCGACCAATT AATATCACCT CACTTGTCCG ATTGTCCACA 
ACAGTACCAA ACACCATTGT TGTTTCTTGG ACTGCAGAAA TTGGAAGAAA CTATTCCATG 
GCAGTGTATC TTGTAAAACA GTTGTCCTCA ACAGTTCTTC TTCAGAGGTT ACGAGCAAAG 
GGAATAAGGA ATCCGGATCA TTCTAGAGCT TTAATTAAAG AGAAATTGAC TGCGGATCCA 
GACAGTGAAA TAGCTACAAC CAGCCTAAGG GTTTCTCTAC TATGTCCACT TGGTAAAATG 
CGGCTGACAA TTCCATGTCG GGCCCTGACA TGTTCTCATC TGCAGTGTTT TGATGCAACT 
CTTTATATTC AGATGAATGA GAAAAAACCA ACCTGGGTTT GTCCTGTCTG TGATAAGAAG 
GCTCCATACG AACACCTTAT TATTGATGGC TTGTTTATGG AAATCCTAAA GTACTGTACA 
GACTGCGATG AGATACAGTT TAAGGAAGAT GGCTCTTGGG CACCTATGAG ATCAAAAAAG 
GAAGTACAGG AAGTCTCTGC CTCGTACAAT GGAGTTGATG GATGCTTGAG CTCCACATTG 
GAGCATCAGG TGGCTTCTCA CCACCAGTCC TCAAATAAAA ATAAGAAAGT AGAGGTGATT 
GACTTAACCA TAGACAGTTC CTCTGATGAA GAGGAGGAAG AGCCTTCTGC CAAGAGGACC 
TGTCCTTCCC TATCTCCCAC ATCACCACTA AATAATAAAG GCATATTAAG TCTTCCACAT 
CAAGCATCTC CAGTGTCCCG CACCCCGAGC CTCCCTGCTG TAGACACAAG CTACATTAAT 
ACATCCCTCA TCCAAGACTA CAGGCATCCT TTCCACATGA CACCCATGCC TTATGACCTA 
CAAGGATTAG ATTTCTTTCC TTTCTTATCA GGAGACAATC AGCATTATAA CACCTCCCTC 
CTGGCTGCTG CTGCAGCAGC AGTGTCAGAT GATCAAGACC TCTTACACTC GTCTCGGTTT 
TTCCCGTATA CCTCCTCGCA GATGTTTCTC GATCAGTTAA GTGCAGGAGG CAGTACTTCT 
CTGCCAACCA CCAATGGAAG CAGTAGTGGC AGTAACAGCA GCCTCGTGTC CTCCAACAGC 
CTGCGGGAGA GCCATAGCCA CACTGTCGCA AACAGGAGCA GCACGGACAC GGCATCCATC 
TTTGGCATCA TACCAGACAT TATTTCATTG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_022412850.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268558.1 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe283741
Clone ID Related Accession (Same CDS sequence)	XM_022412850.1
Accession Version	XM_022412850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGC TGCGGGGCGG GGACGCGGCG GGGCGGGGGG AGAAGCTCCC GGAAAGTTTC 
GTGAGCCCCG GACCGGCGTT GGGGTTCGGC CCGGGGGCGG TGCTGGTGGT GCCGGTGGTG 
CCCGCGATGC TGGTGATGGC TTCTCAAACA GGCAAACACC AGCAAATGGT TATGAGCCTT 
AGAGTTTCTG AACTCCAAGT ACTGTTGGGC TACGCTGGGA GAAACAAGCA CGGACGCAAA 
CACGAACTTC TCACAAAAGC CCTGCATTTG CTAAAGGCTG GCTGCAGTCC TGCTGTGCAA 
ATGAAAATTA AAGAACTCTA TAGGCGGAGG TTCCCCCAGA AAATCATGAC GCCCGCGGAC 
TTGTCCATCC CCAATGTCCA TTCAAGTCCT ATGCCAGCAA CTTTGTCTCC ATCTACCATT 
CCACAGCTCA CTTACGATGG TCACCCTGCA TCATCACCGT TACTCCCTGT TTCTCTTCTG 
GGACCTAAAC ATGAACTGGA ACTCCCACAT CTTACATCAG CTCTTCACCC AGTCCATCCG 
GATATAAAAC TTCAAAAATT ACCATTTTAT GATTTACTGG ATGAACTGAT AAAACCCACC 
AGTCTAGCAT CAGACAACAG TCAGCGCTTT CGAGAAACCT GTTTTGCATT TGCATTGACA 
CCACAACAAG TGCAGCAAAT CAGTAGTTCC ATGGATATTT CTGGGACCAA ATGTGACTTC 
ACAGTGCAGG TCCAGTTAAG GTTTTGTTTA TCAGAAACCA GTTGTCCACA AGAAGATCAT 
TTCCCACCCA ATCTTTGTGT GAAAGTGAAT ACAAAACCTT GCAGCCTCCC GGGTTATCTT 
CCACCTACAA AAAATGGTGT GGAACCAAAG CGACCCAGCC GACCAATTAA TATCACCTCA 
CTTGTCCGAT TGTCCACAAC AGTACCAAAC ACCATTGTTG TTTCTTGGAC TGCAGAAATT 
GGAAGAAACT ATTCCATGGC AGTGTATCTT GTAAAACAGT TGTCCTCAAC AGTTCTTCTT 
CAGAGGTTAC GAGCAAAGGG AATAAGGAAT CCGGATCATT CTAGAGCTTT AATTAAAGAG 
AAATTGACTG CGGATCCAGA CAGTGAAATA GCTACAACCA GCCTAAGGGT TTCTCTACTA 
TGTCCACTTG GTAAAATGCG GCTGACAATT CCATGTCGGG CCCTGACATG TTCTCATCTG 
CAGTGTTTTG ATGCAACTCT TTATATTCAG ATGAATGAGA AAAAACCAAC CTGGGTTTGT 
CCTGTCTGTG ATAAGAAGGC TCCATACGAA CACCTTATTA TTGATGGCTT GTTTATGGAA 
ATCCTAAAGT ACTGTACAGA CTGCGATGAG ATACAGTTTA AGGAAGATGG CTCTTGGGCA 
CCTATGAGAT CAAAAAAGGA AGTACAGGAA GTCTCTGCCT CGTACAATGG AGTTGATGGA 
TGCTTGAGCT CCACATTGGA GCATCAGGTG GCTTCTCACC ACCAGTCCTC AAATAAAAAT 
AAGAAAGTAG AGGTGATTGA CTTAACCATA GACAGTTCCT CTGATGAAGA GGAGGAAGAG 
CCTTCTGCCA AGAGGACCTG TCCTTCCCTA TCTCCCACAT CACCACTAAA TAATAAAGGC 
ATATTAAGTC TTCCACATCA AGCATCTCCA GTGTCCCGCA CCCCGAGCCT CCCTGCTGTA 
GACACAAGCT ACATTAATAC ATCCCTCATC CAAGACTACA GGCATCCTTT CCACATGACA 
CCCATGCCTT ATGACCTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG 
CATTATAACA CCTCCCTCCT GGCTGCTGCT GCAGCAGCAG TGTCAGATGA TCAAGACCTC 
TTACACTCGT CTCGGTTTTT CCCGTATACC TCCTCGCAGA TGTTTCTCGA TCAGTTAAGT 
GCAGGAGGCA GTACTTCTCT GCCAACCACC AATGGAAGCA GTAGTGGCAG TAACAGCAGC 
CTCGTGTCCT CCAACAGCCT GCGGGAGAGC CATAGCCACA CTGTCGCAAA CAGGAGCAGC 
ACGGACACGG CATCCATCTT TGGCATCATA CCAGACATTA TTTCATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268558.1
CDS	17..2110
Translation	MLGLRGGDAAGRGEKLPESFVSPGPALGFGPGAVLVVPVVPAMLVMASQTGKHQQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_022412850.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412850.1

ORF Insert Sequence:

ATGCTGGGGC TGCGGGGCGG GGACGCGGCG GGGCGGGGGG AGAAGCTCCC GGAAAGTTTC 
GTGAGCCCCG GACCGGCGTT GGGGTTCGGC CCGGGGGCGG TGCTGGTGGT GCCGGTGGTG 
CCCGCGATGC TGGTGATGGC TTCTCAAACA GGCAAACACC AGCAAATGGT TATGAGCCTT 
AGAGTTTCTG AACTCCAAGT ACTGTTGGGC TACGCTGGGA GAAACAAGCA CGGACGCAAA 
CACGAACTTC TCACAAAAGC CCTGCATTTG CTAAAGGCTG GCTGCAGTCC TGCTGTGCAA 
ATGAAAATTA AAGAACTCTA TAGGCGGAGG TTCCCCCAGA AAATCATGAC GCCCGCGGAC 
TTGTCCATCC CCAATGTCCA TTCAAGTCCT ATGCCAGCAA CTTTGTCTCC ATCTACCATT 
CCACAGCTCA CTTACGATGG TCACCCTGCA TCATCACCGT TACTCCCTGT TTCTCTTCTG 
GGACCTAAAC ATGAACTGGA ACTCCCACAT CTTACATCAG CTCTTCACCC AGTCCATCCG 
GATATAAAAC TTCAAAAATT ACCATTTTAT GATTTACTGG ATGAACTGAT AAAACCCACC 
AGTCTAGCAT CAGACAACAG TCAGCGCTTT CGAGAAACCT GTTTTGCATT TGCATTGACA 
CCACAACAAG TGCAGCAAAT CAGTAGTTCC ATGGATATTT CTGGGACCAA ATGTGACTTC 
ACAGTGCAGG TCCAGTTAAG GTTTTGTTTA TCAGAAACCA GTTGTCCACA AGAAGATCAT 
TTCCCACCCA ATCTTTGTGT GAAAGTGAAT ACAAAACCTT GCAGCCTCCC GGGTTATCTT 
CCACCTACAA AAAATGGTGT GGAACCAAAG CGACCCAGCC GACCAATTAA TATCACCTCA 
CTTGTCCGAT TGTCCACAAC AGTACCAAAC ACCATTGTTG TTTCTTGGAC TGCAGAAATT 
GGAAGAAACT ATTCCATGGC AGTGTATCTT GTAAAACAGT TGTCCTCAAC AGTTCTTCTT 
CAGAGGTTAC GAGCAAAGGG AATAAGGAAT CCGGATCATT CTAGAGCTTT AATTAAAGAG 
AAATTGACTG CGGATCCAGA CAGTGAAATA GCTACAACCA GCCTAAGGGT TTCTCTACTA 
TGTCCACTTG GTAAAATGCG GCTGACAATT CCATGTCGGG CCCTGACATG TTCTCATCTG 
CAGTGTTTTG ATGCAACTCT TTATATTCAG ATGAATGAGA AAAAACCAAC CTGGGTTTGT 
CCTGTCTGTG ATAAGAAGGC TCCATACGAA CACCTTATTA TTGATGGCTT GTTTATGGAA 
ATCCTAAAGT ACTGTACAGA CTGCGATGAG ATACAGTTTA AGGAAGATGG CTCTTGGGCA 
CCTATGAGAT CAAAAAAGGA AGTACAGGAA GTCTCTGCCT CGTACAATGG AGTTGATGGA 
TGCTTGAGCT CCACATTGGA GCATCAGGTG GCTTCTCACC ACCAGTCCTC AAATAAAAAT 
AAGAAAGTAG AGGTGATTGA CTTAACCATA GACAGTTCCT CTGATGAAGA GGAGGAAGAG 
CCTTCTGCCA AGAGGACCTG TCCTTCCCTA TCTCCCACAT CACCACTAAA TAATAAAGGC 
ATATTAAGTC TTCCACATCA AGCATCTCCA GTGTCCCGCA CCCCGAGCCT CCCTGCTGTA 
GACACAAGCT ACATTAATAC ATCCCTCATC CAAGACTACA GGCATCCTTT CCACATGACA 
CCCATGCCTT ATGACCTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG 
CATTATAACA CCTCCCTCCT GGCTGCTGCT GCAGCAGCAG TGTCAGATGA TCAAGACCTC 
TTACACTCGT CTCGGTTTTT CCCGTATACC TCCTCGCAGA TGTTTCTCGA TCAGTTAAGT 
GCAGGAGGCA GTACTTCTCT GCCAACCACC AATGGAAGCA GTAGTGGCAG TAACAGCAGC 
CTCGTGTCCT CCAACAGCCT GCGGGAGAGC CATAGCCACA CTGTCGCAAA CAGGAGCAGC 
ACGGACACGG CATCCATCTT TGGCATCATA CCAGACATTA TTTCATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_022412851.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268559.1 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe283742
Clone ID Related Accession (Same CDS sequence)	XM_022412851.1
Accession Version	XM_022412851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGC TGCGGGGCGG GGACGCGGCG GGGCGGGGGG AGAAGCTCCC GGAAAGTTTC 
GTGAGCCCCG GACCGGCGTT GGGGTTCGGC CCGGGGGCGG TGCTGGTGGT GCCGGTGGTG 
CCCGCGATGC TGGTGATGGC TTCTCAAACA GGCAAACACC AGCAAATGGT TATGAGCCTT 
AGAGTTTCTG AACTCCAAGT ACTGTTGGGC TACGCTGGGA GAAACAAGCA CGGACGCAAA 
CACGAACTTC TCACAAAAGC CCTGCATTTG CTAAAGGCTG GCTGCAGTCC TGCTGTGCAA 
ATGAAAATTA AAGAACTCTA TAGGCGGAGG TTCCCCCAGA AAATCATGAC GCCCGCGGAC 
TTGTCCATCC CCAATGTCCA TTCAAGTCCT ATGCCAGCAA CTTTGTCTCC ATCTACCATT 
CCACAGCTCA CTTACGATGG TCACCCTGCA TCATCACCGT TACTCCCTGT TTCTCTTCTG 
GGACCTAAAC ATGAACTGGA ACTCCCACAT CTTACATCAG CTCTTCACCC AGTCCATCCG 
GATATAAAAC TTCAAAAATT ACCATTTTAT GATTTACTGG ATGAACTGAT AAAACCCACC 
AGTCTAGCAT CAGACAACAG TCAGCGCTTT CGAGAAACCT GTTTTGCATT TGCATTGACA 
CCACAACAAG TGCAGCAAAT CAGTAGTTCC ATGGATATTT CTGGGACCAA ATGTGACTTC 
ACAGTGCAGG TCCAGTTAAG GTTTTGTTTA TCAGAAACCA GTTGTCCACA AGAAGATCAT 
TTCCCACCCA ATCTTTGTGT GAAAGTGAAT ACAAAACCTT GCAGCCTCCC GGGTTATCTT 
CCACCTACAA AAAATGGTGT GGAACCAAAG CGACCCAGCC GACCAATTAA TATCACCTCA 
CTTGTCCGAT TGTCCACAAC AGTACCAAAC ACCATTGTTG TTTCTTGGAC TGCAGAAATT 
GGAAGAAACT ATTCCATGGC AGTGTATCTT GTAAAACAGT TGTCCTCAAC AGTTCTTCTT 
CAGAGGTTAC GAGCAAAGGG AATAAGGAAT CCGGATCATT CTAGAGCTTT AATTAAAGAG 
AAATTGACTG CGGATCCAGA CAGTGAAATA GCTACAACCA GCCTAAGGGT TTCTCTACTA 
TGTCCACTTG GTAAAATGCG GCTGACAATT CCATGTCGGG CCCTGACATG TTCTCATCTG 
CAGTGTTTTG ATGCAACTCT TTATATTCAG ATGAATGAGA AAAAACCAAC CTGGGTTTGT 
CCTGTCTGTG ATAAGAAGGC TCCATACGAA CACCTTATTA TTGATGGCTT GTTTATGGAA 
ATCCTAAAGT ACTGTACAGA CTGCGATGAG ATACAGTTTA AGGAAGATGG CTCTTGGGCA 
CCTATGAGAT CAAAAAAGGA AGTACAGGAA GTCTCTGCCT CGTACAATGG AGTTGATGGA 
TGCTTGAGCT CCACATTGGA GCATCAGGTG GCTTCTCACC ACCAGTCCTC AAATAAAAAT 
AAGAAAGTAG AGGTGATTGA CTTAACCATA GACAGTTCCT CTGATGAAGA GGAGGAAGAG 
CCTTCTGCCA AGAGGACCTG TCCTTCCCTA TCTCCCACAT CACCACTAAA TAATAAAGGC 
ATATTAAGTC TTCCACATCA AGCATCTCCA GTGTCCCGCA CCCCGAGCCT CCCTGCTGTA 
GACACAAGCT ACATTAATAC ATCCCTCATC CAAGACTACA GGCATCCTTT CCACATGACA 
CCCATGCCTT ATGACCTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG 
ATGTTTCTCG ATCAGTTAAG TGCAGGAGGC AGTACTTCTC TGCCAACCAC CAATGGAAGC 
AGTAGTGGCA GTAACAGCAG CCTCGTGTCC TCCAACAGCC TGCGGGAGAG CCATAGCCAC 
ACTGTCGCAA ACAGGAGCAG CACGGACACG GCATCCATCT TTGGCATCAT ACCAGACATT 
ATTTCATTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268559.1
CDS	17..2011
Translation	MLGLRGGDAAGRGEKLPESFVSPGPALGFGPGAVLVVPVVPAMLVMASQTGKHQQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_022412851.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412851.1

ORF Insert Sequence:

ATGCTGGGGC TGCGGGGCGG GGACGCGGCG GGGCGGGGGG AGAAGCTCCC GGAAAGTTTC 
GTGAGCCCCG GACCGGCGTT GGGGTTCGGC CCGGGGGCGG TGCTGGTGGT GCCGGTGGTG 
CCCGCGATGC TGGTGATGGC TTCTCAAACA GGCAAACACC AGCAAATGGT TATGAGCCTT 
AGAGTTTCTG AACTCCAAGT ACTGTTGGGC TACGCTGGGA GAAACAAGCA CGGACGCAAA 
CACGAACTTC TCACAAAAGC CCTGCATTTG CTAAAGGCTG GCTGCAGTCC TGCTGTGCAA 
ATGAAAATTA AAGAACTCTA TAGGCGGAGG TTCCCCCAGA AAATCATGAC GCCCGCGGAC 
TTGTCCATCC CCAATGTCCA TTCAAGTCCT ATGCCAGCAA CTTTGTCTCC ATCTACCATT 
CCACAGCTCA CTTACGATGG TCACCCTGCA TCATCACCGT TACTCCCTGT TTCTCTTCTG 
GGACCTAAAC ATGAACTGGA ACTCCCACAT CTTACATCAG CTCTTCACCC AGTCCATCCG 
GATATAAAAC TTCAAAAATT ACCATTTTAT GATTTACTGG ATGAACTGAT AAAACCCACC 
AGTCTAGCAT CAGACAACAG TCAGCGCTTT CGAGAAACCT GTTTTGCATT TGCATTGACA 
CCACAACAAG TGCAGCAAAT CAGTAGTTCC ATGGATATTT CTGGGACCAA ATGTGACTTC 
ACAGTGCAGG TCCAGTTAAG GTTTTGTTTA TCAGAAACCA GTTGTCCACA AGAAGATCAT 
TTCCCACCCA ATCTTTGTGT GAAAGTGAAT ACAAAACCTT GCAGCCTCCC GGGTTATCTT 
CCACCTACAA AAAATGGTGT GGAACCAAAG CGACCCAGCC GACCAATTAA TATCACCTCA 
CTTGTCCGAT TGTCCACAAC AGTACCAAAC ACCATTGTTG TTTCTTGGAC TGCAGAAATT 
GGAAGAAACT ATTCCATGGC AGTGTATCTT GTAAAACAGT TGTCCTCAAC AGTTCTTCTT 
CAGAGGTTAC GAGCAAAGGG AATAAGGAAT CCGGATCATT CTAGAGCTTT AATTAAAGAG 
AAATTGACTG CGGATCCAGA CAGTGAAATA GCTACAACCA GCCTAAGGGT TTCTCTACTA 
TGTCCACTTG GTAAAATGCG GCTGACAATT CCATGTCGGG CCCTGACATG TTCTCATCTG 
CAGTGTTTTG ATGCAACTCT TTATATTCAG ATGAATGAGA AAAAACCAAC CTGGGTTTGT 
CCTGTCTGTG ATAAGAAGGC TCCATACGAA CACCTTATTA TTGATGGCTT GTTTATGGAA 
ATCCTAAAGT ACTGTACAGA CTGCGATGAG ATACAGTTTA AGGAAGATGG CTCTTGGGCA 
CCTATGAGAT CAAAAAAGGA AGTACAGGAA GTCTCTGCCT CGTACAATGG AGTTGATGGA 
TGCTTGAGCT CCACATTGGA GCATCAGGTG GCTTCTCACC ACCAGTCCTC AAATAAAAAT 
AAGAAAGTAG AGGTGATTGA CTTAACCATA GACAGTTCCT CTGATGAAGA GGAGGAAGAG 
CCTTCTGCCA AGAGGACCTG TCCTTCCCTA TCTCCCACAT CACCACTAAA TAATAAAGGC 
ATATTAAGTC TTCCACATCA AGCATCTCCA GTGTCCCGCA CCCCGAGCCT CCCTGCTGTA 
GACACAAGCT ACATTAATAC ATCCCTCATC CAAGACTACA GGCATCCTTT CCACATGACA 
CCCATGCCTT ATGACCTACA AGGATTAGAT TTCTTTCCTT TCTTATCAGG AGACAATCAG 
ATGTTTCTCG ATCAGTTAAG TGCAGGAGGC AGTACTTCTC TGCCAACCAC CAATGGAAGC 
AGTAGTGGCA GTAACAGCAG CCTCGTGTCC TCCAACAGCC TGCGGGAGAG CCATAGCCAC 
ACTGTCGCAA ACAGGAGCAG CACGGACACG GCATCCATCT TTGGCATCAT ACCAGACATT 
ATTTCATTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_022412852.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268560.1 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe283743
Clone ID Related Accession (Same CDS sequence)	XM_022412852.1
Accession Version	XM_022412852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA GTTTTTGTTT CTCCGGGATA AATGTCCAAG AGTTGTTGGG TTGTATGCAA 
ATGGTTATGA GCCTTAGAGT TTCTGAACTC CAAGTACTGT TGGGCTACGC TGGGAGAAAC 
AAGCACGGAC GCAAACACGA ACTTCTCACA AAAGCCCTGC ATTTGCTAAA GGCTGGCTGC 
AGTCCTGCTG TGCAAATGAA AATTAAAGAA CTCTATAGGC GGAGGTTCCC CCAGAAAATC 
ATGACGCCCG CGGACTTGTC CATCCCCAAT GTCCATTCAA GTCCTATGCC AGCAACTTTG 
TCTCCATCTA CCATTCCACA GCTCACTTAC GATGGTCACC CTGCATCATC ACCGTTACTC 
CCTGTTTCTC TTCTGGGACC TAAACATGAA CTGGAACTCC CACATCTTAC ATCAGCTCTT 
CACCCAGTCC ATCCGGATAT AAAACTTCAA AAATTACCAT TTTATGATTT ACTGGATGAA 
CTGATAAAAC CCACCAGTCT AGCATCAGAC AACAGTCAGC GCTTTCGAGA AACCTGTTTT 
GCATTTGCAT TGACACCACA ACAAGTGCAG CAAATCAGTA GTTCCATGGA TATTTCTGGG 
ACCAAATGTG ACTTCACAGT GCAGGTCCAG TTAAGGTTTT GTTTATCAGA AACCAGTTGT 
CCACAAGAAG ATCATTTCCC ACCCAATCTT TGTGTGAAAG TGAATACAAA ACCTTGCAGC 
CTCCCGGGTT ATCTTCCACC TACAAAAAAT GGTGTGGAAC CAAAGCGACC CAGCCGACCA 
ATTAATATCA CCTCACTTGT CCGATTGTCC ACAACAGTAC CAAACACCAT TGTTGTTTCT 
TGGACTGCAG AAATTGGAAG AAACTATTCC ATGGCAGTGT ATCTTGTAAA ACAGTTGTCC 
TCAACAGTTC TTCTTCAGAG GTTACGAGCA AAGGGAATAA GGAATCCGGA TCATTCTAGA 
GCTTTAATTA AAGAGAAATT GACTGCGGAT CCAGACAGTG AAATAGCTAC AACCAGCCTA 
AGGGTTTCTC TACTATGTCC ACTTGGTAAA ATGCGGCTGA CAATTCCATG TCGGGCCCTG 
ACATGTTCTC ATCTGCAGTG TTTTGATGCA ACTCTTTATA TTCAGATGAA TGAGAAAAAA 
CCAACCTGGG TTTGTCCTGT CTGTGATAAG AAGGCTCCAT ACGAACACCT TATTATTGAT 
GGCTTGTTTA TGGAAATCCT AAAGTACTGT ACAGACTGCG ATGAGATACA GTTTAAGGAA 
GATGGCTCTT GGGCACCTAT GAGATCAAAA AAGGAAGTAC AGGAAGTCTC TGCCTCGTAC 
AATGGAGTTG ATGGATGCTT GAGCTCCACA TTGGAGCATC AGGTGGCTTC TCACCACCAG 
TCCTCAAATA AAAATAAGAA AGTAGAGGTG ATTGACTTAA CCATAGACAG TTCCTCTGAT 
GAAGAGGAGG AAGAGCCTTC TGCCAAGAGG ACCTGTCCTT CCCTATCTCC CACATCACCA 
CTAAATAATA AAGGCATATT AAGTCTTCCA CATCAAGCAT CTCCAGTGTC CCGCACCCCG 
AGCCTCCCTG CTGTAGACAC AAGCTACATT AATACATCCC TCATCCAAGA CTACAGGCAT 
CCTTTCCACA TGACACCCAT GCCTTATGAC CTACAAGGAT TAGATTTCTT TCCTTTCTTA 
TCAGGAGACA ATCAGCATTA TAACACCTCC CTCCTGGCTG CTGCTGCAGC AGCAGTGTCA 
GATGATCAAG ACCTCTTACA CTCGTCTCGG TTTTTCCCGT ATACCTCCTC GCAGATGTTT 
CTCGATCAGT TAAGTGCAGG AGGCAGTACT TCTCTGCCAA CCACCAATGG AAGCAGTAGT 
GGCAGTAACA GCAGCCTCGT GTCCTCCAAC AGCCTGCGGG AGAGCCATAG CCACACTGTC 
GCAAACAGGA GCAGCACGGA CACGGCATCC ATCTTTGGCA TCATACCAGA CATTATTTCA 
TTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268560.1
CDS	16..2004
Translation	MERSFCFSGINVQELLGCMQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_022412852.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412852.1

ORF Insert Sequence:

ATGGAAAGAA GTTTTTGTTT CTCCGGGATA AATGTCCAAG AGTTGTTGGG TTGTATGCAA 
ATGGTTATGA GCCTTAGAGT TTCTGAACTC CAAGTACTGT TGGGCTACGC TGGGAGAAAC 
AAGCACGGAC GCAAACACGA ACTTCTCACA AAAGCCCTGC ATTTGCTAAA GGCTGGCTGC 
AGTCCTGCTG TGCAAATGAA AATTAAAGAA CTCTATAGGC GGAGGTTCCC CCAGAAAATC 
ATGACGCCCG CGGACTTGTC CATCCCCAAT GTCCATTCAA GTCCTATGCC AGCAACTTTG 
TCTCCATCTA CCATTCCACA GCTCACTTAC GATGGTCACC CTGCATCATC ACCGTTACTC 
CCTGTTTCTC TTCTGGGACC TAAACATGAA CTGGAACTCC CACATCTTAC ATCAGCTCTT 
CACCCAGTCC ATCCGGATAT AAAACTTCAA AAATTACCAT TTTATGATTT ACTGGATGAA 
CTGATAAAAC CCACCAGTCT AGCATCAGAC AACAGTCAGC GCTTTCGAGA AACCTGTTTT 
GCATTTGCAT TGACACCACA ACAAGTGCAG CAAATCAGTA GTTCCATGGA TATTTCTGGG 
ACCAAATGTG ACTTCACAGT GCAGGTCCAG TTAAGGTTTT GTTTATCAGA AACCAGTTGT 
CCACAAGAAG ATCATTTCCC ACCCAATCTT TGTGTGAAAG TGAATACAAA ACCTTGCAGC 
CTCCCGGGTT ATCTTCCACC TACAAAAAAT GGTGTGGAAC CAAAGCGACC CAGCCGACCA 
ATTAATATCA CCTCACTTGT CCGATTGTCC ACAACAGTAC CAAACACCAT TGTTGTTTCT 
TGGACTGCAG AAATTGGAAG AAACTATTCC ATGGCAGTGT ATCTTGTAAA ACAGTTGTCC 
TCAACAGTTC TTCTTCAGAG GTTACGAGCA AAGGGAATAA GGAATCCGGA TCATTCTAGA 
GCTTTAATTA AAGAGAAATT GACTGCGGAT CCAGACAGTG AAATAGCTAC AACCAGCCTA 
AGGGTTTCTC TACTATGTCC ACTTGGTAAA ATGCGGCTGA CAATTCCATG TCGGGCCCTG 
ACATGTTCTC ATCTGCAGTG TTTTGATGCA ACTCTTTATA TTCAGATGAA TGAGAAAAAA 
CCAACCTGGG TTTGTCCTGT CTGTGATAAG AAGGCTCCAT ACGAACACCT TATTATTGAT 
GGCTTGTTTA TGGAAATCCT AAAGTACTGT ACAGACTGCG ATGAGATACA GTTTAAGGAA 
GATGGCTCTT GGGCACCTAT GAGATCAAAA AAGGAAGTAC AGGAAGTCTC TGCCTCGTAC 
AATGGAGTTG ATGGATGCTT GAGCTCCACA TTGGAGCATC AGGTGGCTTC TCACCACCAG 
TCCTCAAATA AAAATAAGAA AGTAGAGGTG ATTGACTTAA CCATAGACAG TTCCTCTGAT 
GAAGAGGAGG AAGAGCCTTC TGCCAAGAGG ACCTGTCCTT CCCTATCTCC CACATCACCA 
CTAAATAATA AAGGCATATT AAGTCTTCCA CATCAAGCAT CTCCAGTGTC CCGCACCCCG 
AGCCTCCCTG CTGTAGACAC AAGCTACATT AATACATCCC TCATCCAAGA CTACAGGCAT 
CCTTTCCACA TGACACCCAT GCCTTATGAC CTACAAGGAT TAGATTTCTT TCCTTTCTTA 
TCAGGAGACA ATCAGCATTA TAACACCTCC CTCCTGGCTG CTGCTGCAGC AGCAGTGTCA 
GATGATCAAG ACCTCTTACA CTCGTCTCGG TTTTTCCCGT ATACCTCCTC GCAGATGTTT 
CTCGATCAGT TAAGTGCAGG AGGCAGTACT TCTCTGCCAA CCACCAATGG AAGCAGTAGT 
GGCAGTAACA GCAGCCTCGT GTCCTCCAAC AGCCTGCGGG AGAGCCATAG CCACACTGTC 
GCAAACAGGA GCAGCACGGA CACGGCATCC ATCTTTGGCA TCATACCAGA CATTATTTCA 
TTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS1 ( XM_022412853.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268561.1 Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe283744
Clone ID Related Accession (Same CDS sequence)	XM_022412853.1
Accession Version	XM_022412853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 SUMO-protein ligase PIAS1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATGA GCCTTAGAGT TTCTGAACTC CAAGTACTGT TGGGCTACGC TGGGAGAAAC 
AAGCACGGAC GCAAACACGA ACTTCTCACA AAAGCCCTGC ATTTGCTAAA GGCTGGCTGC 
AGTCCTGCTG TGCAAATGAA AATTAAAGAA CTCTATAGGC GGAGGTTCCC CCAGAAAATC 
ATGACGCCCG CGGACTTGTC CATCCCCAAT GTCCATTCAA GTCCTATGCC AGCAACTTTG 
TCTCCATCTA CCATTCCACA GCTCACTTAC GATGGTCACC CTGCATCATC ACCGTTACTC 
CCTGTTTCTC TTCTGGGACC TAAACATGAA CTGGAACTCC CACATCTTAC ATCAGCTCTT 
CACCCAGTCC ATCCGGATAT AAAACTTCAA AAATTACCAT TTTATGATTT ACTGGATGAA 
CTGATAAAAC CCACCAGTCT AGCATCAGAC AACAGTCAGC GCTTTCGAGA AACCTGTTTT 
GCATTTGCAT TGACACCACA ACAAGTGCAG CAAATCAGTA GTTCCATGGA TATTTCTGGG 
ACCAAATGTG ACTTCACAGT GCAGGTCCAG TTAAGGTTTT GTTTATCAGA AACCAGTTGT 
CCACAAGAAG ATCATTTCCC ACCCAATCTT TGTGTGAAAG TGAATACAAA ACCTTGCAGC 
CTCCCGGGTT ATCTTCCACC TACAAAAAAT GGTGTGGAAC CAAAGCGACC CAGCCGACCA 
ATTAATATCA CCTCACTTGT CCGATTGTCC ACAACAGTAC CAAACACCAT TGTTGTTTCT 
TGGACTGCAG AAATTGGAAG AAACTATTCC ATGGCAGTGT ATCTTGTAAA ACAGTTGTCC 
TCAACAGTTC TTCTTCAGAG GTTACGAGCA AAGGGAATAA GGAATCCGGA TCATTCTAGA 
GCTTTAATTA AAGAGAAATT GACTGCGGAT CCAGACAGTG AAATAGCTAC AACCAGCCTA 
AGGGTTTCTC TACTATGTCC ACTTGGTAAA ATGCGGCTGA CAATTCCATG TCGGGCCCTG 
ACATGTTCTC ATCTGCAGTG TTTTGATGCA ACTCTTTATA TTCAGATGAA TGAGAAAAAA 
CCAACCTGGG TTTGTCCTGT CTGTGATAAG AAGGCTCCAT ACGAACACCT TATTATTGAT 
GGCTTGTTTA TGGAAATCCT AAAGTACTGT ACAGACTGCG ATGAGATACA GTTTAAGGAA 
GATGGCTCTT GGGCACCTAT GAGATCAAAA AAGGAAGTAC AGGAAGTCTC TGCCTCGTAC 
AATGGAGTTG ATGGATGCTT GAGCTCCACA TTGGAGCATC AGGTGGCTTC TCACCACCAG 
TCCTCAAATA AAAATAAGAA AGTAGAGGTG ATTGACTTAA CCATAGACAG TTCCTCTGAT 
GAAGAGGAGG AAGAGCCTTC TGCCAAGAGG ACCTGTCCTT CCCTATCTCC CACATCACCA 
CTAAATAATA AAGGCATATT AAGTCTTCCA CATCAAGCAT CTCCAGTGTC CCGCACCCCG 
AGCCTCCCTG CTGTAGACAC AAGCTACATT AATACATCCC TCATCCAAGA CTACAGGCAT 
CCTTTCCACA TGACACCCAT GCCTTATGAC CTACAAGGAT TAGATTTCTT TCCTTTCTTA 
TCAGGAGACA ATCAGCATTA TAACACCTCC CTCCTGGCTG CTGCTGCAGC AGCAGTGTCA 
GATGATCAAG ACCTCTTACA CTCGTCTCGG TTTTTCCCGT ATACCTCCTC GCAGATGTTT 
CTCGATCAGT TAAGTGCAGG AGGCAGTACT TCTCTGCCAA CCACCAATGG AAGCAGTAGT 
GGCAGTAACA GCAGCCTCGT GTCCTCCAAC AGCCTGCGGG AGAGCCATAG CCACACTGTC 
GCAAACAGGA GCAGCACGGA CACGGCATCC ATCTTTGGCA TCATACCAGA CATTATTTCA 
TTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268561.1
CDS	136..2064
Translation	MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVANRSSTDTASIFGIIPDIISLD

Target ORF information:

RefSeq Version	XM_022412853.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein inhibitor of activated STAT 1 (PIAS1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412853.1

ORF Insert Sequence:

ATGGTTATGA GCCTTAGAGT TTCTGAACTC CAAGTACTGT TGGGCTACGC TGGGAGAAAC 
AAGCACGGAC GCAAACACGA ACTTCTCACA AAAGCCCTGC ATTTGCTAAA GGCTGGCTGC 
AGTCCTGCTG TGCAAATGAA AATTAAAGAA CTCTATAGGC GGAGGTTCCC CCAGAAAATC 
ATGACGCCCG CGGACTTGTC CATCCCCAAT GTCCATTCAA GTCCTATGCC AGCAACTTTG 
TCTCCATCTA CCATTCCACA GCTCACTTAC GATGGTCACC CTGCATCATC ACCGTTACTC 
CCTGTTTCTC TTCTGGGACC TAAACATGAA CTGGAACTCC CACATCTTAC ATCAGCTCTT 
CACCCAGTCC ATCCGGATAT AAAACTTCAA AAATTACCAT TTTATGATTT ACTGGATGAA 
CTGATAAAAC CCACCAGTCT AGCATCAGAC AACAGTCAGC GCTTTCGAGA AACCTGTTTT 
GCATTTGCAT TGACACCACA ACAAGTGCAG CAAATCAGTA GTTCCATGGA TATTTCTGGG 
ACCAAATGTG ACTTCACAGT GCAGGTCCAG TTAAGGTTTT GTTTATCAGA AACCAGTTGT 
CCACAAGAAG ATCATTTCCC ACCCAATCTT TGTGTGAAAG TGAATACAAA ACCTTGCAGC 
CTCCCGGGTT ATCTTCCACC TACAAAAAAT GGTGTGGAAC CAAAGCGACC CAGCCGACCA 
ATTAATATCA CCTCACTTGT CCGATTGTCC ACAACAGTAC CAAACACCAT TGTTGTTTCT 
TGGACTGCAG AAATTGGAAG AAACTATTCC ATGGCAGTGT ATCTTGTAAA ACAGTTGTCC 
TCAACAGTTC TTCTTCAGAG GTTACGAGCA AAGGGAATAA GGAATCCGGA TCATTCTAGA 
GCTTTAATTA AAGAGAAATT GACTGCGGAT CCAGACAGTG AAATAGCTAC AACCAGCCTA 
AGGGTTTCTC TACTATGTCC ACTTGGTAAA ATGCGGCTGA CAATTCCATG TCGGGCCCTG 
ACATGTTCTC ATCTGCAGTG TTTTGATGCA ACTCTTTATA TTCAGATGAA TGAGAAAAAA 
CCAACCTGGG TTTGTCCTGT CTGTGATAAG AAGGCTCCAT ACGAACACCT TATTATTGAT 
GGCTTGTTTA TGGAAATCCT AAAGTACTGT ACAGACTGCG ATGAGATACA GTTTAAGGAA 
GATGGCTCTT GGGCACCTAT GAGATCAAAA AAGGAAGTAC AGGAAGTCTC TGCCTCGTAC 
AATGGAGTTG ATGGATGCTT GAGCTCCACA TTGGAGCATC AGGTGGCTTC TCACCACCAG 
TCCTCAAATA AAAATAAGAA AGTAGAGGTG ATTGACTTAA CCATAGACAG TTCCTCTGAT 
GAAGAGGAGG AAGAGCCTTC TGCCAAGAGG ACCTGTCCTT CCCTATCTCC CACATCACCA 
CTAAATAATA AAGGCATATT AAGTCTTCCA CATCAAGCAT CTCCAGTGTC CCGCACCCCG 
AGCCTCCCTG CTGTAGACAC AAGCTACATT AATACATCCC TCATCCAAGA CTACAGGCAT 
CCTTTCCACA TGACACCCAT GCCTTATGAC CTACAAGGAT TAGATTTCTT TCCTTTCTTA 
TCAGGAGACA ATCAGCATTA TAACACCTCC CTCCTGGCTG CTGCTGCAGC AGCAGTGTCA 
GATGATCAAG ACCTCTTACA CTCGTCTCGG TTTTTCCCGT ATACCTCCTC GCAGATGTTT 
CTCGATCAGT TAAGTGCAGG AGGCAGTACT TCTCTGCCAA CCACCAATGG AAGCAGTAGT 
GGCAGTAACA GCAGCCTCGT GTCCTCCAAC AGCCTGCGGG AGAGCCATAG CCACACTGTC 
GCAAACAGGA GCAGCACGGA CACGGCATCC ATCTTTGGCA TCATACCAGA CATTATTTCA 
TTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PIAS1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

ONLINE FORM

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