HECTD1 cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe10251 XM_845208.5
Latest version!
Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OCe10251 XM_845208.4 Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe10251
    Clone ID Related Accession (Same CDS sequence) XM_845208.5 , XM_845208.4
    Accession Version XM_845208.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HECTD1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_845208.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACATTGCTA GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC GCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAAGTGA CAGCTCGTGC CATCACGTAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTAGT TGTGGTTGAA CTTAATAACA GGACTAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGTT AGAACTGATA TGTACTCGAG AATCAGGAGC AGTGTTTGAG
    GCTGGTGGTT TGAATCGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTGCATAAA
    GATACCTTGC ACTCTGCCCT GACTGAGGTA TCAAGACTCT GTGGCACGAT GGAACCTCTA
    GATTCTTCTT TAGAAATCAT GGTGGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCCCT ACGGTGCTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCAGCTT GTAAACCTGG TCGCAGCACT
    GCAGGAGCAC CTTCCACGGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGCCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACATGATCT CCTGAGGTCA
    GAACTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGGA GATTGGATAG CTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCCT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTAGTAGC AATTCTTCAG
    TCTCCCGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGC TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAGGCAA GTCTTGCTCT GATTAGGAAA ATGATTCATT TTTGCTCTGA AGCACTTTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CTGTACTAGT GGAAATCACT
    GCAACGGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGAGATTTGG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCAAG ACTTGGTGTA
    ATCAGCAAAG TATCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGG
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACTTGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGTAA AAGAGGCAGA
    AAACTGAAAT CTAAATTAGA AAAGACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CATGGAGAGA TTTAATGAAG ACAGCCTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGATTGGT GCAAGCACTT
    CTTACTGTCT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCTTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCT GTACTAGAGT CCATTGAACG TCTACCTCTT
    CATTTGTATG ACACACCTGG ATCCACATAT AACTTGCAGA TACTCACTAG AAGATTAAGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAAT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGGACGAATG CCAAGACTGC ATATGAATGG GTAAATCCAG CTGCTTATGG ACTTGTAGTA
    GTTACATCTT CAGAAGGAAG AAATCTACCT TATGGCCGTT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC GTCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACGCATG TTGATGACTG CAGTCTCAAT GAACCAGGAT CAACAGCCAC CTGGCCTCTT
    GACCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCTAGTG GACAAACCCA TTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACC
    GTCACAGGGG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCCGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCCGA TAGTTCTGTT CGCACTCCTG GGGAATCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGCTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC CAATTTGACT
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT
    GTGGCCACAG CAACAACAGT CCTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACTACT
    AGCCTCACAT CCACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCTGAATA TTCCTTGTAT
    GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC
    TTGCCTGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAAGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTGCAGC GTCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCACGCTGT CACCTCGCAG CTACCACAAG TTCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAGGAG TATGAAACAA AAGGAGGACG TCGGAGAACA
    TGGGATGATG ATTATGTGCT CAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACG ACTGATCTAG AGATTCCACC CCCAGGAACG
    CCTCATTCAG AGCTCTTAGA AGAAGTGGAA TGCACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATTT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTGGCA
    AAAGAACAGC CTCAGGCCAA AGCTGGCAAT GGACAGAATT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT CTATATAGTT GCAAGTGATC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CCTTGGCAAG TGGAGCTCTA
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTGTATT TCACGTGTAC AGCATTTGGT GCATCAAGAG CAATAGTGTG GTTACAAAAC
    CGACGTGAAG CCACTGTGGA AAGAACAAGA ACCACCAGTA GTGTAAGGCG AGATGATCCT
    GGAGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAAT GGGCTGAAAA CGTCATGCAA ATTCATGCAG ACCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAGGA AGGAACTGGT TTGGGCCCTA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATACTATGTG
    CAGAGATCGT GTGGACTGTT CACAGCACCA TTTCCGCAAG ACAGTGATGA GCTTGAAAGG
    ATCACGAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTTA TGTGCATGGG TGATATTAAA
    AGCAACATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA TTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CCAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC AGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACGAA ATTACAAGAA CTAGTGCTAA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AGTATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAC AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAAGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCCGGTTTC
    CTGAGGTTTG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGCTCAAC TCTGCCCCCC GGTGGACTAG CTAATCTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCCACTGAT GCAAGCTATC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCTGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACCATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_850301.2
    CDS457..8289
    Translation

    Target ORF information:

    RefSeq Version XM_845208.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_845208.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACATTGCTA GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC GCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAAGTGA CAGCTCGTGC CATCACGTAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTAGT TGTGGTTGAA CTTAATAACA GGACTAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGTT AGAACTGATA TGTACTCGAG AATCAGGAGC AGTGTTTGAG
    GCTGGTGGTT TGAATCGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTGCATAAA
    GATACCTTGC ACTCTGCCCT GACTGAGGTA TCAAGACTCT GTGGCACGAT GGAACCTCTA
    GATTCTTCTT TAGAAATCAT GGTGGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCCCT ACGGTGCTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCAGCTT GTAAACCTGG TCGCAGCACT
    GCAGGAGCAC CTTCCACGGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGCCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACATGATCT CCTGAGGTCA
    GAACTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGGA GATTGGATAG CTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCCT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTAGTAGC AATTCTTCAG
    TCTCCCGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGC TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAGGCAA GTCTTGCTCT GATTAGGAAA ATGATTCATT TTTGCTCTGA AGCACTTTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CTGTACTAGT GGAAATCACT
    GCAACGGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGAGATTTGG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCAAG ACTTGGTGTA
    ATCAGCAAAG TATCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGG
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACTTGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGTAA AAGAGGCAGA
    AAACTGAAAT CTAAATTAGA AAAGACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CATGGAGAGA TTTAATGAAG ACAGCCTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGATTGGT GCAAGCACTT
    CTTACTGTCT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCTTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCT GTACTAGAGT CCATTGAACG TCTACCTCTT
    CATTTGTATG ACACACCTGG ATCCACATAT AACTTGCAGA TACTCACTAG AAGATTAAGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAAT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGGACGAATG CCAAGACTGC ATATGAATGG GTAAATCCAG CTGCTTATGG ACTTGTAGTA
    GTTACATCTT CAGAAGGAAG AAATCTACCT TATGGCCGTT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC GTCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACGCATG TTGATGACTG CAGTCTCAAT GAACCAGGAT CAACAGCCAC CTGGCCTCTT
    GACCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCTAGTG GACAAACCCA TTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACC
    GTCACAGGGG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCCGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCCGA TAGTTCTGTT CGCACTCCTG GGGAATCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGCTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC CAATTTGACT
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT
    GTGGCCACAG CAACAACAGT CCTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACTACT
    AGCCTCACAT CCACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCTGAATA TTCCTTGTAT
    GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC
    TTGCCTGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAAGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTGCAGC GTCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCACGCTGT CACCTCGCAG CTACCACAAG TTCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAGGAG TATGAAACAA AAGGAGGACG TCGGAGAACA
    TGGGATGATG ATTATGTGCT CAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACG ACTGATCTAG AGATTCCACC CCCAGGAACG
    CCTCATTCAG AGCTCTTAGA AGAAGTGGAA TGCACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATTT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTGGCA
    AAAGAACAGC CTCAGGCCAA AGCTGGCAAT GGACAGAATT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT CTATATAGTT GCAAGTGATC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CCTTGGCAAG TGGAGCTCTA
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTGTATT TCACGTGTAC AGCATTTGGT GCATCAAGAG CAATAGTGTG GTTACAAAAC
    CGACGTGAAG CCACTGTGGA AAGAACAAGA ACCACCAGTA GTGTAAGGCG AGATGATCCT
    GGAGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAAT GGGCTGAAAA CGTCATGCAA ATTCATGCAG ACCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAGGA AGGAACTGGT TTGGGCCCTA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATACTATGTG
    CAGAGATCGT GTGGACTGTT CACAGCACCA TTTCCGCAAG ACAGTGATGA GCTTGAAAGG
    ATCACGAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTTA TGTGCATGGG TGATATTAAA
    AGCAACATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA TTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CCAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC AGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACGAA ATTACAAGAA CTAGTGCTAA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AGTATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAC AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAAGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCCGGTTTC
    CTGAGGTTTG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGCTCAAC TCTGCCCCCC GGTGGACTAG CTAATCTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCCACTGAT GCAAGCTATC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCTGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACCATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10251
    Clone ID Related Accession (Same CDS sequence) XM_845208.5 , XM_845208.4
    Accession Version XM_845208.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HECTD1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845208.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACATTGCTA GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC GCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAAGTGA CAGCTCGTGC CATCACGTAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTAGT TGTGGTTGAA CTTAATAACA GGACTAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGTT AGAACTGATA TGTACTCGAG AATCAGGAGC AGTGTTTGAG
    GCTGGTGGTT TGAATCGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTGCATAAA
    GATACCTTGC ACTCTGCCCT GACTGAGGTA TCAAGACTCT GTGGCACGAT GGAACCTCTA
    GATTCTTCTT TAGAAATCAT GGTGGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCCCT ACGGTGCTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCAGCTT GTAAACCTGG TCGCAGCACT
    GCAGGAGCAC CTTCCACGGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGCCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACATGATCT CCTGAGGTCA
    GAACTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGGA GATTGGATAG CTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCCT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTAGTAGC AATTCTTCAG
    TCTCCCGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGC TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAGGCAA GTCTTGCTCT GATTAGGAAA ATGATTCATT TTTGCTCTGA AGCACTTTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CTGTACTAGT GGAAATCACT
    GCAACGGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGAGATTTGG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCAAG ACTTGGTGTA
    ATCAGCAAAG TATCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGG
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACTTGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGTAA AAGAGGCAGA
    AAACTGAAAT CTAAATTAGA AAAGACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CATGGAGAGA TTTAATGAAG ACAGCCTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGATTGGT GCAAGCACTT
    CTTACTGTCT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCTTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCT GTACTAGAGT CCATTGAACG TCTACCTCTT
    CATTTGTATG ACACACCTGG ATCCACATAT AACTTGCAGA TACTCACTAG AAGATTAAGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAAT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGGACGAATG CCAAGACTGC ATATGAATGG GTAAATCCAG CTGCTTATGG ACTTGTAGTA
    GTTACATCTT CAGAAGGAAG AAATCTACCT TATGGCCGTT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC GTCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACGCATG TTGATGACTG CAGTCTCAAT GAACCAGGAT CAACAGCCAC CTGGCCTCTT
    GACCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCTAGTG GACAAACCCA TTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACC
    GTCACAGGGG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCCGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCCGA TAGTTCTGTT CGCACTCCTG GGGAATCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGCTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC CAATTTGACT
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT
    GTGGCCACAG CAACAACAGT CCTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACTACT
    AGCCTCACAT CCACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCTGAATA TTCCTTGTAT
    GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC
    TTGCCTGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAAGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTGCAGC GTCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCACGCTGT CACCTCGCAG CTACCACAAG TTCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAGGAG TATGAAACAA AAGGAGGACG TCGGAGAACA
    TGGGATGATG ATTATGTGCT CAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACG ACTGATCTAG AGATTCCACC CCCAGGAACG
    CCTCATTCAG AGCTCTTAGA AGAAGTGGAA TGCACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATTT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTGGCA
    AAAGAACAGC CTCAGGCCAA AGCTGGCAAT GGACAGAATT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT CTATATAGTT GCAAGTGATC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CCTTGGCAAG TGGAGCTCTA
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTGTATT TCACGTGTAC AGCATTTGGT GCATCAAGAG CAATAGTGTG GTTACAAAAC
    CGACGTGAAG CCACTGTGGA AAGAACAAGA ACCACCAGTA GTGTAAGGCG AGATGATCCT
    GGAGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAAT GGGCTGAAAA CGTCATGCAA ATTCATGCAG ACCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAGGA AGGAACTGGT TTGGGCCCTA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATACTATGTG
    CAGAGATCGT GTGGACTGTT CACAGCACCA TTTCCGCAAG ACAGTGATGA GCTTGAAAGG
    ATCACGAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTTA TGTGCATGGG TGATATTAAA
    AGCAACATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA TTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CCAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC AGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACGAA ATTACAAGAA CTAGTGCTAA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AGTATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAC AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAAGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCCGGTTTC
    CTGAGGTTTG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGCTCAAC TCTGCCCCCC GGTGGACTAG CTAATCTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCCACTGAT GCAAGCTATC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCTGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACCATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_850301.2
    CDS456..8288
    Translation

    Target ORF information:

    RefSeq Version XM_845208.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_845208.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACATTGCTA GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC GCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAAGTGA CAGCTCGTGC CATCACGTAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTAGT TGTGGTTGAA CTTAATAACA GGACTAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGTT AGAACTGATA TGTACTCGAG AATCAGGAGC AGTGTTTGAG
    GCTGGTGGTT TGAATCGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTGCATAAA
    GATACCTTGC ACTCTGCCCT GACTGAGGTA TCAAGACTCT GTGGCACGAT GGAACCTCTA
    GATTCTTCTT TAGAAATCAT GGTGGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCCCT ACGGTGCTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCAGCTT GTAAACCTGG TCGCAGCACT
    GCAGGAGCAC CTTCCACGGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGCCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACATGATCT CCTGAGGTCA
    GAACTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGGA GATTGGATAG CTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCCT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTAGTAGC AATTCTTCAG
    TCTCCCGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGC TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAGGCAA GTCTTGCTCT GATTAGGAAA ATGATTCATT TTTGCTCTGA AGCACTTTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CTGTACTAGT GGAAATCACT
    GCAACGGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGAGATTTGG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCAAG ACTTGGTGTA
    ATCAGCAAAG TATCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGG
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACTTGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGTAA AAGAGGCAGA
    AAACTGAAAT CTAAATTAGA AAAGACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CATGGAGAGA TTTAATGAAG ACAGCCTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGATTGGT GCAAGCACTT
    CTTACTGTCT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCTTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCT GTACTAGAGT CCATTGAACG TCTACCTCTT
    CATTTGTATG ACACACCTGG ATCCACATAT AACTTGCAGA TACTCACTAG AAGATTAAGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAAT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGGACGAATG CCAAGACTGC ATATGAATGG GTAAATCCAG CTGCTTATGG ACTTGTAGTA
    GTTACATCTT CAGAAGGAAG AAATCTACCT TATGGCCGTT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC GTCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACGCATG TTGATGACTG CAGTCTCAAT GAACCAGGAT CAACAGCCAC CTGGCCTCTT
    GACCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCTAGTG GACAAACCCA TTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACC
    GTCACAGGGG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCCGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCCGA TAGTTCTGTT CGCACTCCTG GGGAATCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGCTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC CAATTTGACT
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT
    GTGGCCACAG CAACAACAGT CCTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACTACT
    AGCCTCACAT CCACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCTGAATA TTCCTTGTAT
    GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC
    TTGCCTGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAAGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTGCAGC GTCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCACGCTGT CACCTCGCAG CTACCACAAG TTCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAGGAG TATGAAACAA AAGGAGGACG TCGGAGAACA
    TGGGATGATG ATTATGTGCT CAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACG ACTGATCTAG AGATTCCACC CCCAGGAACG
    CCTCATTCAG AGCTCTTAGA AGAAGTGGAA TGCACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATTT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTGGCA
    AAAGAACAGC CTCAGGCCAA AGCTGGCAAT GGACAGAATT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT CTATATAGTT GCAAGTGATC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CCTTGGCAAG TGGAGCTCTA
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTGTATT TCACGTGTAC AGCATTTGGT GCATCAAGAG CAATAGTGTG GTTACAAAAC
    CGACGTGAAG CCACTGTGGA AAGAACAAGA ACCACCAGTA GTGTAAGGCG AGATGATCCT
    GGAGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAAT GGGCTGAAAA CGTCATGCAA ATTCATGCAG ACCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAGGA AGGAACTGGT TTGGGCCCTA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATACTATGTG
    CAGAGATCGT GTGGACTGTT CACAGCACCA TTTCCGCAAG ACAGTGATGA GCTTGAAAGG
    ATCACGAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTTA TGTGCATGGG TGATATTAAA
    AGCAACATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA TTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CCAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC AGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACGAA ATTACAAGAA CTAGTGCTAA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AGTATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAC AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAAGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCCGGTTTC
    CTGAGGTTTG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGCTCAAC TCTGCCCCCC GGTGGACTAG CTAATCTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCCACTGAT GCAAGCTATC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCTGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACCATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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