NF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NF1
Entrez Gene ID	480618
Full Name	neurofibromin 1
General protein information	Preferred Names neurofibromin 1 Names neurofibromin neurofibromatosis type 1 neurofibromin 1 (neurofibromatosis, von Recklinghausen disease, Watson disease)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022423325.1	XP_022279033.1	neurofibromin isoform X2
XM_022423327.1	XP_022279035.1	neurofibromin isoform X4
XM_022423324.1	XP_022279032.1	neurofibromin isoform X1
XM_022423329.1	XP_022279037.1	neurofibromin isoform X6
XM_014116492.1	XP_013971967.1	neurofibromin
XM_022423326.1	XP_022279034.1	neurofibromin isoform X3
XM_022423328.1	XP_022279036.1	neurofibromin isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	NF1	NP_001035957.1
Rattus norvegicus (Norway rat)	Nf1	NP_036741.1
Danio rerio (zebrafish)	nf1b	XP_692937.4
Drosophila melanogaster (fruit fly)	Nf1	NP_733132.2
	NF1	XP_002800429.1
Canis lupus familiaris (dog)	NF1	XP_537738.3
Mus musculus (house mouse)	Nf1	NP_035027.1
Gallus gallus (chicken)	NF1	XP_004946843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003140	XP_312829.4
Xenopus tropicalis (tropical clawed frog)	nf1	XP_002936865.2
Pan troglodytes (chimpanzee)	NF1	XP_511395.2

Gene Ontology

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe290773	XM_022423324.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe290774	XM_022423325.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe290775	XM_022423326.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe290776	XM_022423327.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe290777	XM_022423328.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe290778	XM_022423329.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe38290	XM_014116492.1 Latest version!	Canis lupus familiaris neurofibromin 1 (NF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NF1 ( XM_022423324.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279032.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X1, mRNA.

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CloneID	OCe290773
Clone ID Related Accession (Same CDS sequence)	XM_022423324.1
Accession Version	XM_022423324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA 
AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT 
GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA 
GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC 
CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT 
CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT 
GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA 
AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA 
CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA 
GGACCCTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT 
GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC 
CTGACACCCA CTTCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAT 
CTTAACCTTT CTAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGGAATCGAC 
AAGGAGAACG TTGAACTCTC CCCAACCACT GGGCACTGTA ACAGTGGACG GACTCGCCAT 
GGATCCGCAA GCCAAGTGCA GAAGCAAAGA AGCGCTGGCA GTTTCAAACG TAATAGCATT 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279032.1
CDS	148..8502
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_022423324.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423324.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA 
AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT 
GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA 
GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC 
CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT 
CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT 
GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA 
AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA 
CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA 
GGACCCTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT 
GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC 
CTGACACCCA CTTCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAT 
CTTAACCTTT CTAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGGAATCGAC 
AAGGAGAACG TTGAACTCTC CCCAACCACT GGGCACTGTA ACAGTGGACG GACTCGCCAT 
GGATCCGCAA GCCAAGTGCA GAAGCAAAGA AGCGCTGGCA GTTTCAAACG TAATAGCATT 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_022423325.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279033.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X2, mRNA.

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CloneID	OCe290774
Clone ID Related Accession (Same CDS sequence)	XM_022423325.1
Accession Version	XM_022423325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGGCCGAT 
AGAAGTTCCT GTCACTTTCT CTTCCTTTAT GGGGTAGGAT GTGATGTTCC TTCAAGTGGA 
ACTACTAGTC AGATGTCCAT GGATCATGAA GAATTACTAC GTTCTTGTAC TCCTGGAGCC 
TCTCTCCGGA AGGGAAAAGG AAACTCATCC ATGGATAGTG CAGCAGGGTG CAGCGGTACT 
CCGCCAATAT GCCGACAAGC TCAGACTAAA CTAGAAGTGG CCCTGTACAT GTTTCTGTGG 
AGCCCTGACA CTGAAGCTGT TCTGGTTGCC ATGTCCTGTT TCCGTCACCT TTGTGAGGAA 
GCAGATATCC GGTGTGGGGT GGACGAAGTG TCGGTGCACA ACTTCTTGCC CAACTATAAC 
ACATTTATGG AATTTGCCTC TGTCAGCAAT ATGATGTCAA CAGGAAGAGC AGCACTTCAG 
AAAAGAGTGA TGGCACTACT AAGGCGCATT GAGCATCCCA CTGCAGGAAA TACCGAGGCT 
TGGGAAGATA CACATGCGAA GTGGGAACAA GCTACAAAAC TAATCCTCAA CTACCCAAAA 
GCTAAAATGG AAGATGGCCA GGCTGCTGAA AGCCTTCACA AGACCATTGT TAAGAGGCGA 
ATGTCTCATG TCAGTGGAGG AGGATCCATA GATTTGTCTG ACACGGACTC CCTACAGGAA 
TGGATCAACA TGACTGGCTT CCTCTGTGCC CTTGGGGGAG TGTGCCTGCA GCAGAGAAGC 
AATTCTGGCT TGGCAACCTA TAGCCCACCC ATGGGCCCAG TCAGTGAACG TAAGGGTTCC 
ATGATTTCAG TGATGTCTTC AGAGGGAAAT GCAGATACAC CTGTCAGCAA ATTTATGGAT 
CGGCTGTTAT CCTTAATGGT GTGTAACCAT GAGAAAGTGG GACTTCAAAT ACGGACCAAT 
GTTAAGGATC TGGTGGGTCT AGAATTGAGT CCTGCTCTTT ATCCAATGCT ATTTAACAAA 
TTGAAGAATA CCATCAGCAA GTTTTTTGAC TCCCAAGGAC AGGTTTTATT GACTGACACC 
AATACTCAGT TTGTGGAGCA AACCATAGCT ATAATGAAGA ACTTACTAGA TAATCATACT 
GAAGGCAGCT CTGAACACCT GGGGCAAGCT AGCATTGAAA CAATGATGTT AAATCTAGTC 
AGATATGTTC GTGTGCTTGG GAATATGGTC CATGCAATTC AAATAAAAAC GAAACTCTGT 
CAGTTAGTCG AGGTGATGAT GGCGAGGAGA GATGACCTCT CATTTTGTCA AGAGATGAAA 
TTTAGGAATA AGATGGTAGA ATACCTGACA GACTGGGTTA TGGGAACATC CAACCAGGCA 
GCAGATGATG ATGTAAAATG TCTTACAAGA GACTTGGACC AGGCAAGCAT GGAAGCAGTA 
GTTTCACTCC TGGCTGGTCT TCCACTGCAG CCGGAGGAAG GAGATGGTGT GGAATTGATG 
GAAGCCAAGT CACAGTTATT TCTTAAGTAT TTCACATTGT TCATGAACCT ATTGAATGAC 
TGTAGTGAAG TTGAAGATGA AAATGCACAA ACAGGTGGCA GGAAACGTGG CATGTCTCGG 
AGGCTGGCAT CACTGAGGCA CTGTACAGTC CTTGCAATGT CAAACTTACT CAATGCTAAT 
GTGGACAGTG GCCTCATGCA TTCCATAGGT TTAGGTTATC ACAAAGATCT TCAGACAAGA 
GCTACATTTA TGGAAGTTCT GACAAAAATC CTCCAACAAG GCACAGAATT TGACACACTT 
GCGGAAACAG TGCTGGCCGA CCGGTTTGAG AGGCTGGTGG AGTTGGTGAC GATGATGGGA 
GACCAGGGAG AGCTCCCAAT AGCCATGGCT CTGGCCAACG TGGTCCCGTG TTCCCAGTGG 
GATGAACTAG CTCGAGTTTT GGTGACTCTG TTTGATTCTC GGCATTTACT CTACCAATTG 
CTCTGGAACA TGTTTTCTAA GGAAGTAGAA TTGGCAGACT CCATGCAGAC TCTTTTCCGA 
GGAAACAGCT TAGCCAGTAA AATAATGACC TTCTGTTTCA AGGTATATGG TGCTACCTAC 
CTACAAAAAC TCTTGGATCC GTTATTACGA ATTGTGATCA CATCCTCTGA TTGGCAGCAT 
GTTAGCTTTG AAGTGGATCC TACCAGATTA GAACAGTCAG AGAGCCTTGA GGAAAACCAA 
CGGAACCTTC TTCAGATGAC TGAAAAGTTC TTCCATGCCA TCATCAGTTC CTCCTCAGAA 
TTCCCCCCTC AGCTTCGAAG TGTGTGCCAC TGTTTATACC AGGCAACTTG CCACTCCCTA 
CTGAATAAAG CTACAGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTGGT TAGCCAGCGT 
TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT 
GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA 
AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA 
AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG 
AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT 
TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG 
AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC 
GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT 
ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT 
CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA 
GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT 
CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA 
GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA 
GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG 
CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT 
GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC 
CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA 
GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG 
GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG 
AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG 
TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT 
GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT 
ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT 
CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA 
GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA 
GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG 
TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA 
GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT 
AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA 
TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA 
ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA 
GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA 
ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC 
GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG 
GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC 
TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT 
TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC 
TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT 
GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT 
TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT 
ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT 
GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC 
AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG 
AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA 
GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT 
TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG 
CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA 
GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT 
TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA 
GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA 
CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG 
GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA 
CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT 
CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT 
CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC 
CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG 
TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG 
CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT 
TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279033.1
CDS	148..8499
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_022423325.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423325.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGGCCGAT 
AGAAGTTCCT GTCACTTTCT CTTCCTTTAT GGGGTAGGAT GTGATGTTCC TTCAAGTGGA 
ACTACTAGTC AGATGTCCAT GGATCATGAA GAATTACTAC GTTCTTGTAC TCCTGGAGCC 
TCTCTCCGGA AGGGAAAAGG AAACTCATCC ATGGATAGTG CAGCAGGGTG CAGCGGTACT 
CCGCCAATAT GCCGACAAGC TCAGACTAAA CTAGAAGTGG CCCTGTACAT GTTTCTGTGG 
AGCCCTGACA CTGAAGCTGT TCTGGTTGCC ATGTCCTGTT TCCGTCACCT TTGTGAGGAA 
GCAGATATCC GGTGTGGGGT GGACGAAGTG TCGGTGCACA ACTTCTTGCC CAACTATAAC 
ACATTTATGG AATTTGCCTC TGTCAGCAAT ATGATGTCAA CAGGAAGAGC AGCACTTCAG 
AAAAGAGTGA TGGCACTACT AAGGCGCATT GAGCATCCCA CTGCAGGAAA TACCGAGGCT 
TGGGAAGATA CACATGCGAA GTGGGAACAA GCTACAAAAC TAATCCTCAA CTACCCAAAA 
GCTAAAATGG AAGATGGCCA GGCTGCTGAA AGCCTTCACA AGACCATTGT TAAGAGGCGA 
ATGTCTCATG TCAGTGGAGG AGGATCCATA GATTTGTCTG ACACGGACTC CCTACAGGAA 
TGGATCAACA TGACTGGCTT CCTCTGTGCC CTTGGGGGAG TGTGCCTGCA GCAGAGAAGC 
AATTCTGGCT TGGCAACCTA TAGCCCACCC ATGGGCCCAG TCAGTGAACG TAAGGGTTCC 
ATGATTTCAG TGATGTCTTC AGAGGGAAAT GCAGATACAC CTGTCAGCAA ATTTATGGAT 
CGGCTGTTAT CCTTAATGGT GTGTAACCAT GAGAAAGTGG GACTTCAAAT ACGGACCAAT 
GTTAAGGATC TGGTGGGTCT AGAATTGAGT CCTGCTCTTT ATCCAATGCT ATTTAACAAA 
TTGAAGAATA CCATCAGCAA GTTTTTTGAC TCCCAAGGAC AGGTTTTATT GACTGACACC 
AATACTCAGT TTGTGGAGCA AACCATAGCT ATAATGAAGA ACTTACTAGA TAATCATACT 
GAAGGCAGCT CTGAACACCT GGGGCAAGCT AGCATTGAAA CAATGATGTT AAATCTAGTC 
AGATATGTTC GTGTGCTTGG GAATATGGTC CATGCAATTC AAATAAAAAC GAAACTCTGT 
CAGTTAGTCG AGGTGATGAT GGCGAGGAGA GATGACCTCT CATTTTGTCA AGAGATGAAA 
TTTAGGAATA AGATGGTAGA ATACCTGACA GACTGGGTTA TGGGAACATC CAACCAGGCA 
GCAGATGATG ATGTAAAATG TCTTACAAGA GACTTGGACC AGGCAAGCAT GGAAGCAGTA 
GTTTCACTCC TGGCTGGTCT TCCACTGCAG CCGGAGGAAG GAGATGGTGT GGAATTGATG 
GAAGCCAAGT CACAGTTATT TCTTAAGTAT TTCACATTGT TCATGAACCT ATTGAATGAC 
TGTAGTGAAG TTGAAGATGA AAATGCACAA ACAGGTGGCA GGAAACGTGG CATGTCTCGG 
AGGCTGGCAT CACTGAGGCA CTGTACAGTC CTTGCAATGT CAAACTTACT CAATGCTAAT 
GTGGACAGTG GCCTCATGCA TTCCATAGGT TTAGGTTATC ACAAAGATCT TCAGACAAGA 
GCTACATTTA TGGAAGTTCT GACAAAAATC CTCCAACAAG GCACAGAATT TGACACACTT 
GCGGAAACAG TGCTGGCCGA CCGGTTTGAG AGGCTGGTGG AGTTGGTGAC GATGATGGGA 
GACCAGGGAG AGCTCCCAAT AGCCATGGCT CTGGCCAACG TGGTCCCGTG TTCCCAGTGG 
GATGAACTAG CTCGAGTTTT GGTGACTCTG TTTGATTCTC GGCATTTACT CTACCAATTG 
CTCTGGAACA TGTTTTCTAA GGAAGTAGAA TTGGCAGACT CCATGCAGAC TCTTTTCCGA 
GGAAACAGCT TAGCCAGTAA AATAATGACC TTCTGTTTCA AGGTATATGG TGCTACCTAC 
CTACAAAAAC TCTTGGATCC GTTATTACGA ATTGTGATCA CATCCTCTGA TTGGCAGCAT 
GTTAGCTTTG AAGTGGATCC TACCAGATTA GAACAGTCAG AGAGCCTTGA GGAAAACCAA 
CGGAACCTTC TTCAGATGAC TGAAAAGTTC TTCCATGCCA TCATCAGTTC CTCCTCAGAA 
TTCCCCCCTC AGCTTCGAAG TGTGTGCCAC TGTTTATACC AGGCAACTTG CCACTCCCTA 
CTGAATAAAG CTACAGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTGGT TAGCCAGCGT 
TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT 
GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA 
AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA 
AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG 
AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT 
TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG 
AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC 
GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT 
ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT 
CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA 
GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT 
CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA 
GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA 
GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG 
CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT 
GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC 
CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA 
GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG 
GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG 
AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG 
TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT 
GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT 
ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT 
CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA 
GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA 
GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG 
TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA 
GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT 
AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA 
TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA 
ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA 
GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA 
ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC 
GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG 
GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC 
TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT 
TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC 
TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT 
GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT 
TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT 
ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT 
GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC 
AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG 
AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA 
GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT 
TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG 
CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA 
GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT 
TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA 
GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA 
CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG 
GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA 
CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT 
CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT 
CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC 
CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG 
TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG 
CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT 
TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_022423326.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279034.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X3, mRNA.

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CloneID	OCe290775
Clone ID Related Accession (Same CDS sequence)	XM_022423326.1
Accession Version	XM_022423326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATT CTGCATTGGA TTGGTGGCCT 
AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT 
CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA 
TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA 
AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG 
CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA 
GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG 
GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA 
GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC 
CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG 
AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT 
TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT 
GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA 
TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT 
AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT 
GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA 
GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC 
AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC 
ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA 
CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT 
GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC 
ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT 
GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA 
CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA 
AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC 
CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG 
AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT 
GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA 
GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA 
GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG 
GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG 
AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG 
ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA 
AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG 
CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG 
TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA 
CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA 
GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA 
GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA 
ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT 
GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG 
GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT 
CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA 
GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG 
ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA 
CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA 
TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG 
TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC 
CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG 
GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG 
ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA 
CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT 
GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT 
TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT 
GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC 
AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC 
CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC 
AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG 
GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA 
TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC 
CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT 
ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT 
TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG 
GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG 
CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA 
CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG 
CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC 
TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT 
TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC 
CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC 
CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT 
CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA 
GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT 
TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC 
CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA 
TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT 
TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT 
GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG 
AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT 
CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA 
ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG 
GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA 
ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG 
CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT 
GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG 
GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA 
AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT 
GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG 
TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC 
ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG 
CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA 
CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA 
CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC 
AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT 
AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC 
GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA 
GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT 
CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC 
TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT 
GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC 
TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG 
TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT 
ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT 
AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA 
GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC 
ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT 
CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA 
CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC 
TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG 
GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA 
CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA 
GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC 
AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG 
CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT 
TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA 
GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA 
ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA 
GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT 
GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT 
GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA 
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279034.1
CDS	148..8472
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_022423326.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423326.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATT CTGCATTGGA TTGGTGGCCT 
AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT 
CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA 
TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA 
AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG 
CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA 
GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG 
GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA 
GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC 
CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG 
AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT 
TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT 
GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA 
TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT 
AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT 
GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA 
GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC 
AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC 
ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA 
CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT 
GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC 
ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT 
GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA 
CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA 
AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC 
CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG 
AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT 
GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA 
GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA 
GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG 
GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG 
AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG 
ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA 
AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG 
CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG 
TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA 
CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA 
GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA 
GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA 
ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT 
GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG 
GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT 
CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA 
GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG 
ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA 
CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA 
TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG 
TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC 
CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG 
GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG 
ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA 
CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT 
GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT 
TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT 
GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC 
AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC 
CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC 
AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG 
GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA 
TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC 
CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT 
ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT 
TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG 
GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG 
CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA 
CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG 
CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC 
TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT 
TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC 
CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC 
CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT 
CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA 
GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT 
TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC 
CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA 
TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT 
TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT 
GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG 
AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT 
CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA 
ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG 
GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA 
ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG 
CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT 
GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG 
GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA 
AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT 
GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG 
TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC 
ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG 
CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA 
CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA 
CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC 
AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT 
AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC 
GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA 
GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT 
CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC 
TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT 
GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC 
TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG 
TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT 
ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT 
AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA 
GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC 
ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT 
CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA 
CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC 
TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG 
GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA 
CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA 
GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC 
AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG 
CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT 
TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA 
GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA 
ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA 
GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT 
GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT 
GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA 
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_022423327.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279035.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X4, mRNA.

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CloneID	OCe290776
Clone ID Related Accession (Same CDS sequence)	XM_022423327.1
Accession Version	XM_022423327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGTGGT TAGCCAGCGT 
TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT 
GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA 
AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA 
AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG 
AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT 
TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG 
AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC 
GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT 
ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT 
CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA 
GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT 
CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA 
GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA 
GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG 
CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT 
GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC 
CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA 
GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG 
GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG 
AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG 
TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT 
GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT 
ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT 
CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA 
GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA 
GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG 
TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA 
GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT 
AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA 
TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA 
ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA 
GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA 
ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC 
GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG 
GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC 
TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT 
TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC 
TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT 
GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT 
TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT 
ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT 
GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC 
AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG 
AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA 
GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT 
TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG 
CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA 
GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT 
TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA 
GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA 
CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG 
GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA 
CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT 
CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT 
CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC 
CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG 
TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG 
CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT 
TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279035.1
CDS	148..8439
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_022423327.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423327.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGTGGT TAGCCAGCGT 
TTCCCTCAGA ACAGCATCGG TGCAGTGGGA AGTGCCATGT TCCTCAGATT TATCAATCCT 
GCCATTGTCT CACCGTACGA AGCAGGGATT TTAGATAAAA AGCCACCACC TAGAATTGAA 
AGGGGCTTGA AGTTAATGTC AAAGATACTT CAGAGTATTG CCAATCATGT TCTTTTCACA 
AAAGAAGAGC ATATGCGGCC TTTTAATGAT TTTGTGAAGA GCAACTTTGA TGCAGCACGG 
AGGTTTTTCC TTGATATAGC ATCTGATTGT CCTACAAGTG ATACAGTAAA TCATAGTCTT 
TCCTTCATCA GTGATGGCAA TGTGCTTGCT TTACATCGTC TACTCTGGAA CAATCAAGAG 
AAAATTGGCC AGTATCTTTC CAGCAACAGG GATCACAAAG CTGTTGGAAG ACGACCTTTC 
GATAAGATGG CAACACTTCT TGCATACCTG GGTCCTCCAG AGCACAAACC TGTGGCAGAT 
ACACACTGGT CCAGCCTTAA CCTTACCAGT TCAAAGTTTG AGGAATTTAT GACTAGGCAT 
CAAGTACATG AAAAAGAAGA GTTCAAGGCT TTGAAAACAT TAAGTATTTT CTACCAAGCA 
GGGACTTCCA AAGCTGGGAA TCCTATATTT TATTATGTTG CACGGAGGTT TAAAACTGGT 
CAAATCAATG GCGATTTGCT GATATACCAT GTCTTGCTGA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AAATTGTAGT GGACCTTACG CATACTGGGC CGAGCAATCG CTTTAAAACA 
GACTTTCTCT CCAAGTGGTT TGTTGTTTTT CCTGGCTTCG CTTATGACAA TGTCTCTGCA 
GTCTATATCT ATAACTGTAA CTCCTGGGTC AGAGAGTACA CCAAGTATCA CGAGCGGCTG 
CTGACTGGCC TCAAAGGCAG CAAAAGGCTC ATCTTCATAG ACTGTCCTGG GAAACTGGCT 
GAGCACATAG AGCATGAACA ACAGAAACTG CCTGCTGCCA CACTGGCTTT AGAAGAGGAC 
CTGAAGGTAT TCCACAATGC TCTCAAGCTA GCTCACAAAG ACACCAAGGT TTCCATTAAA 
GTTGGTTCTA CTGCTGTTCA AGTAACCTCA GCAGAGCGAA CAAAAGTCCT GGGGCAATCG 
GTCTTTCTAA ATGATATTTA TTATGCTTCG GAAATTGAAG AGATCTGCCT GGTAGATGAG 
AACCAGTTTA CCTTAACCAT TGCAAACCAG GGCACACCAC TCACCTTCAT GCACCAGGAG 
TGTGAAGCCA TTGTTCAGTC TATCATCCAC ATCCGGACAC GCTGGGAGCT GTCACAACCT 
GACTCCATCC CCCAACACAC CAAGATTCGG CCAAAAGATG TCCCTGGGAC GCTGCTCAAT 
ATTGCGTTAC TTAATTTAGG CAGTTCAGAC CCTAGTTTAC GGTCAGCTGC CTATAATCTT 
CTGTGTGCCT TAACTTGTAC CTTCAATTTA AAAATCGAGG GCCAATTACT AGAGACATCA 
GGTTTATGCA TCCCTGCCAA CAACACCCTC TTTATTGTCT CTATTAGTAA GACACTGGCA 
GCCAATGAGC CACATCTGAC CTTAGAATTT TTGGAAGAGT GTATTTCTGG ATTTAGCAAG 
TCTAGTATTG AATTGAAACA CCTTTGTTTG GAATACATGA CTCCGTGGCT GTCAAATCTA 
GTCCGTTTTT GTAAGCACAA TGATGATGCC AAAAGACAGA GAGTTACTGC TATTCTTGAT 
AAGCTGATAA CAATGACCAT AAATGAGAAA CAGATGTACC CATCTATCCA GGCCAAAATA 
TGGGGGAGCC TTGGGCAGAT TACAGATCTC CTTGATGTTG TATTAGACAG TTTCATCAAA 
ACCAGTGCAA CAGGTGGCTT GGGATCAATA AAAGCTGAGG TGATGGCAGA TACTGCTGTA 
GCTTTGGCTT CTGGAAACGT GAAATTGGTT TCAAGCAAGG TTATTGGAAG GATGTGCAAA 
ATAATTGACA AGACATGTTT ATCTCCAACT CCTACCTTAG AGCAACATCT TATGTGGGAC 
GATATTGCTA TTCTAGCACG CTACATGCTG ATGCTGTCCT TCAACAATTC CCTTGATGTG 
GCAGCTCATC TCCCCTACCT CTTCCATGTT GTTACTTTCT TAGTAGCTAC AGGGCCACTC 
TCCCTTAGAG CTTCCACACA TGGATTGGTC ATTAATATCA TTCACTCTCT GTGTACTTGT 
TCACAGCTTC ATTTTAGTGA AGAGACAAAG CAAGTTTTGA GACTCAGTTT GACAGAGTTC 
TCATTACCCA AGTTTTACCT GCTGTTTGGC ATTAGTAAAG TCAAGTCAGC TGCCGTCATT 
GCCTTTCGCT CCAGTTACCG GGACAGGTCA TTCTCTCCTG GCTCCTATGA AAGGGAGACT 
TTTGCTTTGA CATCCTTGGA AACAGTCACA GAAGCTTTGT TGGAGATTAT GGAGGCATGT 
ATGAGAGATA TTCCAACATG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAAAGATTT 
GCATTCCAAT ATAATCCATC CCTGCAACCA AGAGCTCTTG TTGTCTTTGG CTGTATTAGC 
AAACGAGTGT CTCATGGGCA GATAAAACAG ATTATCCGTA TTCTTAGCAA GGCACTTGAG 
AGTTGCTTAA AAGGACCTGA TACTTACAAC AGTCAAGTTC TGATAGAAGC TACAGTAATA 
GCACTAACCA AATTACAACC ACTTCTTAAT AAGGACTCCC CTCTGCACAA AGCCCTCTTT 
TGGGTGGCTG TGGCTGTGCT ACAGCTCGAT GAGGTCAACC TGTATTCAGC AGGCAGTGCG 
CTTCTGGAAC AAAACCTGCA CACCTTAGAC AGTCTGCGGG TATTCAATGA CAAGAGTCCA 
GAGGAAGTAT TTATGGCAAT CCGGAATCCT CTGGAATGGC ACTGCAAACA AATGGATCAT 
TTTGTTGGAC TCAATTTCAA CTCTAACTTT AACTTCGCAC TGGTTGGACA CCTCTTAAAA 
GGGTACAGGC ATCCTTCACC TGCCATTGTG GCAAGAACAG TCAGAATTTT GCATACACTA 
CTAACTCTGG TGAACAAACA TAGAAACTGT GACAAATTTG AGGTGAACAC ACAGAGCGTG 
GCTTATTTAG CAGCCTTACT CACAGTGTCT GAGGAGGTTC GAAGTCGCTG TAGCCTAAAA 
CATAGAAAAT CTCTTCTTCT TACTGATATT TCAATGGAAA ATGTTCCTAT GGATACGTAT 
CCCATCCATC ATGGCGACCC TAGCTATAGG ACGCTAAAGG AGAACCAGCC GTGGTCATCT 
CCCAAAGGTT CTGAAGGGTA CCTTGCAGCC TCCTATCCAG CCGTGGGCCA GACCAGTCCC 
CGAGCCAGGA AATCCATGAG TTTGGACATG GGGCAGCCTT CTCAGGCCAA TACGAAGAAG 
TTGCTGGGAA CAAGGAAAAG TTTTGATCAC TTGATATCAG ACACAAAGGC TCCTAAAAGG 
CAAGAAATGG AATCAGGGAT CACAACACCC CCCAAAATGA GGAGAGTAGC AGAAACTGAT 
TATGAAATGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_022423328.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279036.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X5, mRNA.

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CloneID	OCe290777
Clone ID Related Accession (Same CDS sequence)	XM_022423328.1
Accession Version	XM_022423328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279036.1
CDS	148..8379
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_022423328.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423328.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG AAACTCAGAG GATTTCCTCA TCACAGCAAC ACCCACATTT ACGCAAAGTT 
TCAGTGTCTG AATCAAATGT TCTCTTGGAT GAAGAAGTAC TTACTGATCC GAAGATCCAA 
GCACTTCTTC TTACTGTTCT GGCTACACTG GTAAAATACA CCACAGATGA GTTTGATCAG 
CGAATTCTTT ATGAATACTT AGCAGAAGCC AGTGTTGTTT TCCCCAAGGT TTTTCCTGTT 
GTGCACAATT TGTTGGACTC TAAGATCAAC ACCTTGTTGT CATTGTGCCA AGATCCAAAT 
TTGTTAAATC CAATCCATGG AATCGTGCAG AGTGTGGTAT ACCATGAAGA ATCCCCACCT 
CAGTACCAAA CATCTTATCT GCAAAGTTTT GGTTTTAATG GCTTATGGCG GTTTGCAGGA 
CCCTTTTCAA AGCAAACACA AATCCCAGAC TATGCTGAGC TTATTGTTAA GTTTCTTGAT 
GCCTTGATTG ACACGTATTT GCCTGGAATT GATGAAGAAA CCAGTGAGGA GTCCCTCCTG 
ACACCCACTT CTCCTTATCC TCCTGCAGTG CAGAGCCAGC TTAGTATTAC TGCTAATCTT 
AACCTTTCTA ATTCCATGAC CTCACTTGCA ACTTCCCAGC ATTCCCCAGG AATCGACAAG 
GAGAACGTTG AACTCTCCCC AACCACTGGG CACTGTAACA GTGGACGGAC TCGCCATGGA 
TCCGCAAGCC AAGTGCAGAA GCAAAGAAGC GCTGGCAGTT TCAAACGTAA TAGCATTAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_022423329.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279037.1 Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X6, mRNA.

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CloneID	OCe290778
Clone ID Related Accession (Same CDS sequence)	XM_022423329.1
Accession Version	XM_022423329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neurofibromin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA 
AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT 
GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA 
GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC 
CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT 
CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT 
GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA 
AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA 
CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA 
GGACCCTTTT CAAAGGAATC GACAAGGAGA ACGTTGAACT CTCCCCAACC ACTGGGCACT 
GTAACAGTGG ACGGACTCGC CATGGATCCG CAAGCCAAGT GCAGAAGCAA AGAAGCGCTG 
GCAGTTTCAA ACGTAATAGC ATTAAGAAGA TCGTGTGAAA CTTTCTTTCT TTCCTTTTTC 
AAACAGACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279037.1
CDS	148..8319
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKESTRRTLNSPQPLGTVTVDGLAMDPQAKCRSKEALAVSNVIALRRSCETFFLSFFKQT

Target ORF information:

RefSeq Version	XM_022423329.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423329.1

ORF Insert Sequence:

ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATTATATTG 
GATACACTGG AAAAATGTCT TGCTGGGCAA CCAAAGGACA CGATGAGATT AGATGAAACA 
ATGCTGGTCA AACAGTTGCT GCCGGAAATC TGCCATTTTC TTCATACCTG TCGTGAAGGA 
AACCAACATG CCGCTGAACT TCGGAATTCC GCCTCTGGGG TTTTATTTTC TCTCAGCTGC 
AACAACTTCA ATGCAGTGTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACGGTTTGT 
TCAGAAGACA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT 
GCAAAATTAA AACGACTTCT AAAGGAAACT GCATTTAAAT TTAAAGCCCT AAAGAAGGTT 
GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT AGAAAATTAT 
CCAGATGAAT TTACAAAGCT GTACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA 
AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG 
CCACTGCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TTCAGGATAT ATCCAAGGAT 
GTGGTTGATG AAAACAACAT GAATAAGAAA TTGTTTCTGG ACAGTCTACG AAAAGCACTC 
GCCGGCCACG GAGGAAGCAG GCAGCTGACA GAAAGTGCTG CGATTGCCTG TGTCAAACTC 
TGTAAAGCAA GTACTTATAT CAATTGGGAA GATAACTCTG TCATTTTCCT ACTGGTTCAG 
TCCATGGTGG TTGATCTTAA GAACCTACTC TTTAATCCAA GTAAACCATT CTCAAGAGGC 
AGTCAGCCTG CAGATGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGAATAAGC 
CCTCACAATA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCCCCTTC TACATTTCAC 
TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CTGCATTGGA TTGGTGGCCT AAGATTGATG CTGTATATTG TCACTCAGTT 
GAGCTTCGAA ACATGTTTGG TGAAACACTT CATAAAGCAG TGCAGGGTTG TGGAGCACAC 
CCAGCAATAC GAATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT 
AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAAT ATCTTCTCTT ATCCATGGTG 
AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CAAGAAAACA GGGACCTGAA 
ACCCAAGGCA GTACAGCGGA GTTAATTACA GGACTTGTCC AGCTGGTCCC TCAGTCACAT 
ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TTCTTCATCA GTTAGACAGC 
ATTGATTTGT GGAATCCTGA TGCTCCTGTA GAAACATTTT GGGAGATTAG CTCTCAAATG 
CTTTTTTACA TCTGCAAGAA ATTAACTAGC CATCAGATGC TTAGTAGCAC AGAAATTCTC 
AAATGGTTGA GGGAGATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCC 
GATAGAAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCAAGT 
GGAACTACTA GTCAGATGTC CATGGATCAT GAAGAATTAC TACGTTCTTG TACTCCTGGA 
GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCAGCAGG GTGCAGCGGT 
ACTCCGCCAA TATGCCGACA AGCTCAGACT AAACTAGAAG TGGCCCTGTA CATGTTTCTG 
TGGAGCCCTG ACACTGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGTCA CCTTTGTGAG 
GAAGCAGATA TCCGGTGTGG GGTGGACGAA GTGTCGGTGC ACAACTTCTT GCCCAACTAT 
AACACATTTA TGGAATTTGC CTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT 
CAGAAAAGAG TGATGGCACT ACTAAGGCGC ATTGAGCATC CCACTGCAGG AAATACCGAG 
GCTTGGGAAG ATACACATGC GAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTACCCA 
AAAGCTAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ACAAGACCAT TGTTAAGAGG 
CGAATGTCTC ATGTCAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTACAG 
GAATGGATCA ACATGACTGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA 
AGCAATTCTG GCTTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGTAAGGGT 
TCCATGATTT CAGTGATGTC TTCAGAGGGA AATGCAGATA CACCTGTCAG CAAATTTATG 
GATCGGCTGT TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGGACC 
AATGTTAAGG ATCTGGTGGG TCTAGAATTG AGTCCTGCTC TTTATCCAAT GCTATTTAAC 
AAATTGAAGA ATACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC 
ACCAATACTC AGTTTGTGGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT 
ACTGAAGGCA GCTCTGAACA CCTGGGGCAA GCTAGCATTG AAACAATGAT GTTAAATCTA 
GTCAGATATG TTCGTGTGCT TGGGAATATG GTCCATGCAA TTCAAATAAA AACGAAACTC 
TGTCAGTTAG TCGAGGTGAT GATGGCGAGG AGAGATGACC TCTCATTTTG TCAAGAGATG 
AAATTTAGGA ATAAGATGGT AGAATACCTG ACAGACTGGG TTATGGGAAC ATCCAACCAG 
GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGACTTGG ACCAGGCAAG CATGGAAGCA 
GTAGTTTCAC TCCTGGCTGG TCTTCCACTG CAGCCGGAGG AAGGAGATGG TGTGGAATTG 
ATGGAAGCCA AGTCACAGTT ATTTCTTAAG TATTTCACAT TGTTCATGAA CCTATTGAAT 
GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAACG TGGCATGTCT 
CGGAGGCTGG CATCACTGAG GCACTGTACA GTCCTTGCAA TGTCAAACTT ACTCAATGCT 
AATGTGGACA GTGGCCTCAT GCATTCCATA GGTTTAGGTT ATCACAAAGA TCTTCAGACA 
AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA 
CTTGCGGAAA CAGTGCTGGC CGACCGGTTT GAGAGGCTGG TGGAGTTGGT GACGATGATG 
GGAGACCAGG GAGAGCTCCC AATAGCCATG GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG 
TGGGATGAAC TAGCTCGAGT TTTGGTGACT CTGTTTGATT CTCGGCATTT ACTCTACCAA 
TTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC 
CGAGGAAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC 
TACCTACAAA AACTCTTGGA TCCGTTATTA CGAATTGTGA TCACATCCTC TGATTGGCAG 
CATGTTAGCT TTGAAGTGGA TCCTACCAGA TTAGAACAGT CAGAGAGCCT TGAGGAAAAC 
CAACGGAACC TTCTTCAGAT GACTGAAAAG TTCTTCCATG CCATCATCAG TTCCTCCTCA 
GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC 
CTACTGAATA AAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG 
CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTTATCAAT 
CCTGCCATTG TCTCACCGTA CGAAGCAGGG ATTTTAGATA AAAAGCCACC ACCTAGAATT 
GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTTTTC 
ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AGAGCAACTT TGATGCAGCA 
CGGAGGTTTT TCCTTGATAT AGCATCTGAT TGTCCTACAA GTGATACAGT AAATCATAGT 
CTTTCCTTCA TCAGTGATGG CAATGTGCTT GCTTTACATC GTCTACTCTG GAACAATCAA 
GAGAAAATTG GCCAGTATCT TTCCAGCAAC AGGGATCACA AAGCTGTTGG AAGACGACCT 
TTCGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA 
GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TTGAGGAATT TATGACTAGG 
CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCTTTGAAAA CATTAAGTAT TTTCTACCAA 
GCAGGGACTT CCAAAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTTAAAACT 
GGTCAAATCA ATGGCGATTT GCTGATATAC CATGTCTTGC TGACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACGCATACTG GGCCGAGCAA TCGCTTTAAA 
ACAGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TCGCTTATGA CAATGTCTCT 
GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGAGAGT ACACCAAGTA TCACGAGCGG 
CTGCTGACTG GCCTCAAAGG CAGCAAAAGG CTCATCTTCA TAGACTGTCC TGGGAAACTG 
GCTGAGCACA TAGAGCATGA ACAACAGAAA CTGCCTGCTG CCACACTGGC TTTAGAAGAG 
GACCTGAAGG TATTCCACAA TGCTCTCAAG CTAGCTCACA AAGACACCAA GGTTTCCATT 
AAAGTTGGTT CTACTGCTGT TCAAGTAACC TCAGCAGAGC GAACAAAAGT CCTGGGGCAA 
TCGGTCTTTC TAAATGATAT TTATTATGCT TCGGAAATTG AAGAGATCTG CCTGGTAGAT 
GAGAACCAGT TTACCTTAAC CATTGCAAAC CAGGGCACAC CACTCACCTT CATGCACCAG 
GAGTGTGAAG CCATTGTTCA GTCTATCATC CACATCCGGA CACGCTGGGA GCTGTCACAA 
CCTGACTCCA TCCCCCAACA CACCAAGATT CGGCCAAAAG ATGTCCCTGG GACGCTGCTC 
AATATTGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT 
CTTCTGTGTG CCTTAACTTG TACCTTCAAT TTAAAAATCG AGGGCCAATT ACTAGAGACA 
TCAGGTTTAT GCATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG 
GCAGCCAATG AGCCACATCT GACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC 
AAGTCTAGTA TTGAATTGAA ACACCTTTGT TTGGAATACA TGACTCCGTG GCTGTCAAAT 
CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AGAGAGTTAC TGCTATTCTT 
GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT CCAGGCCAAA 
ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTATTAGA CAGTTTCATC 
AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCTG AGGTGATGGC AGATACTGCT 
GTAGCTTTGG CTTCTGGAAA CGTGAAATTG GTTTCAAGCA AGGTTATTGG AAGGATGTGC 
AAAATAATTG ACAAGACATG TTTATCTCCA ACTCCTACCT TAGAGCAACA TCTTATGTGG 
GACGATATTG CTATTCTAGC ACGCTACATG CTGATGCTGT CCTTCAACAA TTCCCTTGAT 
GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT GTTGTTACTT TCTTAGTAGC TACAGGGCCA 
CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGTACT 
TGTTCACAGC TTCATTTTAG TGAAGAGACA AAGCAAGTTT TGAGACTCAG TTTGACAGAG 
TTCTCATTAC CCAAGTTTTA CCTGCTGTTT GGCATTAGTA AAGTCAAGTC AGCTGCCGTC 
ATTGCCTTTC GCTCCAGTTA CCGGGACAGG TCATTCTCTC CTGGCTCCTA TGAAAGGGAG 
ACTTTTGCTT TGACATCCTT GGAAACAGTC ACAGAAGCTT TGTTGGAGAT TATGGAGGCA 
TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAAAGA 
TTTGCATTCC AATATAATCC ATCCCTGCAA CCAAGAGCTC TTGTTGTCTT TGGCTGTATT 
AGCAAACGAG TGTCTCATGG GCAGATAAAA CAGATTATCC GTATTCTTAG CAAGGCACTT 
GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA 
ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC 
TTTTGGGTGG CTGTGGCTGT GCTACAGCTC GATGAGGTCA ACCTGTATTC AGCAGGCAGT 
GCGCTTCTGG AACAAAACCT GCACACCTTA GACAGTCTGC GGGTATTCAA TGACAAGAGT 
CCAGAGGAAG TATTTATGGC AATCCGGAAT CCTCTGGAAT GGCACTGCAA ACAAATGGAT 
CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTCG CACTGGTTGG ACACCTCTTA 
AAAGGGTACA GGCATCCTTC ACCTGCCATT GTGGCAAGAA CAGTCAGAAT TTTGCATACA 
CTACTAACTC TGGTGAACAA ACATAGAAAC TGTGACAAAT TTGAGGTGAA CACACAGAGC 
GTGGCTTATT TAGCAGCCTT ACTCACAGTG TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA 
AAACATAGAA AATCTCTTCT TCTTACTGAT ATTTCAATGG AAAATGTTCC TATGGATACG 
TATCCCATCC ATCATGGCGA CCCTAGCTAT AGGACGCTAA AGGAGAACCA GCCGTGGTCA 
TCTCCCAAAG GTTCTGAAGG GTACCTTGCA GCCTCCTATC CAGCCGTGGG CCAGACCAGT 
CCCCGAGCCA GGAAATCCAT GAGTTTGGAC ATGGGGCAGC CTTCTCAGGC CAATACGAAG 
AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACAAA GGCTCCTAAA 
AGGCAAGAAA TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT 
GATTATGAAA TGGAAACTCA GAGGATTTCC TCATCACAGC AACACCCACA TTTACGCAAA 
GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA TCCGAAGATC 
CAAGCACTTC TTCTTACTGT TCTGGCTACA CTGGTAAAAT ACACCACAGA TGAGTTTGAT 
CAGCGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT 
GTTGTGCACA ATTTGTTGGA CTCTAAGATC AACACCTTGT TGTCATTGTG CCAAGATCCA 
AATTTGTTAA ATCCAATCCA TGGAATCGTG CAGAGTGTGG TATACCATGA AGAATCCCCA 
CCTCAGTACC AAACATCTTA TCTGCAAAGT TTTGGTTTTA ATGGCTTATG GCGGTTTGCA 
GGACCCTTTT CAAAGGAATC GACAAGGAGA ACGTTGAACT CTCCCCAACC ACTGGGCACT 
GTAACAGTGG ACGGACTCGC CATGGATCCG CAAGCCAAGT GCAGAAGCAA AGAAGCGCTG 
GCAGTTTCAA ACGTAATAGC ATTAAGAAGA TCGTGTGAAA CTTTCTTTCT TTCCTTTTTC 
AAACAGACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_014116492.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971967.1 Canis lupus familiaris neurofibromin 1 (NF1), mRNA.

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CloneID	OCe38290
Clone ID Related Accession (Same CDS sequence)	XM_014116492.1
Accession Version	XM_014116492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	neurofibromin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTGTTGC TTCCAATAAA AACAGGACAG CAGAACACGC ATACCAAAGT CAGTACTGAA 
CACAACAAGG AATGTCTTAT TAATATTTCC AAATACAAGT TTTCTTTGGT TATCAGCGGC 
CTCACCACTA TCTTAAAGAA TGTTAACAAT ATGAGAATAT TTGGAGAAGC TGCTGAAAAA 
AATTTATATC TCTCTCAGTT GATTATATTG GATACACTGG AAAAATGTCT TGCTGGGCAA 
CCAAAGGACA CGATGAGATT AGATGAAACA ATGCTGGTCA AACAGTTGCT GCCGGAAATC 
TGCCATTTTC TTCATACCTG TCGTGAAGGA AACCAACATG CCGCTGAACT TCGGAATTCC 
GCCTCTGGGG TTTTATTTTC TCTCAGCTGC AACAACTTCA ATGCAGTGTT TAGTCGCATT 
TCTACCAGGT TACAGGAATT GACGGTTTGT TCAGAAGACA ATGTTGATGT TCATGATATA 
GAATTGTTAC AGTATATCAA TGTGGATTGT GCAAAATTAA AACGACTTCT AAAGGAAACT 
GCATTTAAAT TTAAAGCCCT AAAGAAGGTT GCACAGTTAG CAGTTATAAA TAGCCTGGAA 
AAAGCATTTT GGAACTGGGT AGAAAATTAT CCAGATGAAT TTACAAAGCT GTACCAGATT 
CCACAGACTG ATATGGCTGA GTGTGCGGAA AAACTGTTTG ACTTGGTGGA TGGTTTTGCT 
GAAAGCACCA AACGTAAAGC AGCAGTTTGG CCACTGCAAA TCATTCTCCT TATCTTGTGT 
CCAGAAATAA TTCAGGATAT ATCCAAGGAT GTGGTTGATG AAAACAACAT GAATAAGAAA 
TTGTTTCTGG ACAGTCTACG AAAAGCACTC GCCGGCCACG GAGGAAGCAG GCAGCTGACA 
GAAAGTGCTG CGATTGCCTG TGTCAAACTC TGTAAAGCAA GTACTTATAT CAATTGGGAA 
GATAACTCTG TCATTTTCCT ACTGGTTCAG TCCATGGTGG TTGATCTTAA GAACCTACTC 
TTTAATCCAA GTAAACCATT CTCAAGAGGC AGTCAGCCTG CAGATGTGGA TCTAATGATT 
GACTGCCTTG TTTCTTGCTT TCGAATAAGC CCTCACAATA ACCAACACTT TAAGATCTGC 
CTGGCTCAGA ATTCCCCTTC TACATTTCAC TATGTGCTGG TAAATTCACT CCATCGAATC 
ATCACCAATA GCACTTTCAA GCATGGACTT GGCACTGCTT CTGCATTGGA TTGGTGGCCT 
AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT 
CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA 
TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA 
AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG 
CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA 
GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG 
GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA 
GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC 
CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG 
AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT 
TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT 
GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA 
TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT 
AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT 
GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA 
GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC 
AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC 
ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA 
CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT 
GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC 
ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT 
GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA 
CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA 
AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC 
CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG 
AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT 
GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA 
GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA 
GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG 
GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG 
AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG 
ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA 
AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG 
CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG 
TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA 
CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA 
GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA 
GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA 
ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT 
GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG 
GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT 
CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA 
GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG 
ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA 
CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA 
TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG 
TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC 
CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG 
GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG 
ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA 
CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT 
GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT 
TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT 
GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC 
AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC 
CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC 
AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG 
GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA 
TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC 
CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT 
ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT 
TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG 
GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG 
CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA 
CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG 
CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC 
TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT 
TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC 
CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC 
CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT 
CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA 
GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT 
TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC 
CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA 
TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT 
TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT 
GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG 
AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT 
CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA 
ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG 
GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA 
ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG 
CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT 
GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG 
GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA 
AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT 
GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG 
TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC 
ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG 
CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA 
CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA 
CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC 
AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT 
AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC 
GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA 
GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT 
CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC 
TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT 
GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC 
TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG 
TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT 
ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT 
AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA 
GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC 
ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT 
CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA 
CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC 
TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG 
GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA 
CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA 
GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC 
AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG 
CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT 
TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA 
GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA 
ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA 
GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT 
GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT 
GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA 
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971967.1
CDS	1..8505
Translation	MLLLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLYGVGCDVPSSGTTSQMSMDHEELLRSCTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEQSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDTVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGSALLEQNLHTLDSLRVFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKENQPWSSPKGSEGYLAASYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_014116492.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris neurofibromin 1 (NF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116492.1

ORF Insert Sequence:

ATGTTGTTGC TTCCAATAAA AACAGGACAG CAGAACACGC ATACCAAAGT CAGTACTGAA 
CACAACAAGG AATGTCTTAT TAATATTTCC AAATACAAGT TTTCTTTGGT TATCAGCGGC 
CTCACCACTA TCTTAAAGAA TGTTAACAAT ATGAGAATAT TTGGAGAAGC TGCTGAAAAA 
AATTTATATC TCTCTCAGTT GATTATATTG GATACACTGG AAAAATGTCT TGCTGGGCAA 
CCAAAGGACA CGATGAGATT AGATGAAACA ATGCTGGTCA AACAGTTGCT GCCGGAAATC 
TGCCATTTTC TTCATACCTG TCGTGAAGGA AACCAACATG CCGCTGAACT TCGGAATTCC 
GCCTCTGGGG TTTTATTTTC TCTCAGCTGC AACAACTTCA ATGCAGTGTT TAGTCGCATT 
TCTACCAGGT TACAGGAATT GACGGTTTGT TCAGAAGACA ATGTTGATGT TCATGATATA 
GAATTGTTAC AGTATATCAA TGTGGATTGT GCAAAATTAA AACGACTTCT AAAGGAAACT 
GCATTTAAAT TTAAAGCCCT AAAGAAGGTT GCACAGTTAG CAGTTATAAA TAGCCTGGAA 
AAAGCATTTT GGAACTGGGT AGAAAATTAT CCAGATGAAT TTACAAAGCT GTACCAGATT 
CCACAGACTG ATATGGCTGA GTGTGCGGAA AAACTGTTTG ACTTGGTGGA TGGTTTTGCT 
GAAAGCACCA AACGTAAAGC AGCAGTTTGG CCACTGCAAA TCATTCTCCT TATCTTGTGT 
CCAGAAATAA TTCAGGATAT ATCCAAGGAT GTGGTTGATG AAAACAACAT GAATAAGAAA 
TTGTTTCTGG ACAGTCTACG AAAAGCACTC GCCGGCCACG GAGGAAGCAG GCAGCTGACA 
GAAAGTGCTG CGATTGCCTG TGTCAAACTC TGTAAAGCAA GTACTTATAT CAATTGGGAA 
GATAACTCTG TCATTTTCCT ACTGGTTCAG TCCATGGTGG TTGATCTTAA GAACCTACTC 
TTTAATCCAA GTAAACCATT CTCAAGAGGC AGTCAGCCTG CAGATGTGGA TCTAATGATT 
GACTGCCTTG TTTCTTGCTT TCGAATAAGC CCTCACAATA ACCAACACTT TAAGATCTGC 
CTGGCTCAGA ATTCCCCTTC TACATTTCAC TATGTGCTGG TAAATTCACT CCATCGAATC 
ATCACCAATA GCACTTTCAA GCATGGACTT GGCACTGCTT CTGCATTGGA TTGGTGGCCT 
AAGATTGATG CTGTATATTG TCACTCAGTT GAGCTTCGAA ACATGTTTGG TGAAACACTT 
CATAAAGCAG TGCAGGGTTG TGGAGCACAC CCAGCAATAC GAATGGCACC GAGTCTTACA 
TTTAAAGAAA AAGTAACAAG CCTTAAATTT AAAGAAAAAC CTACAGACCT GGAGACAAGA 
AGCTATAAAT ATCTTCTCTT ATCCATGGTG AAACTAATTC ATGCAGATCC AAAGCTCTTG 
CTTTGTAATC CAAGAAAACA GGGACCTGAA ACCCAAGGCA GTACAGCGGA GTTAATTACA 
GGACTTGTCC AGCTGGTCCC TCAGTCACAT ATGCCAGAGA TTGCTCAGGA AGCAATGGAG 
GCTCTGCTGG TTCTTCATCA GTTAGACAGC ATTGATTTGT GGAATCCTGA TGCTCCTGTA 
GAAACATTTT GGGAGATTAG CTCTCAAATG CTTTTTTACA TCTGCAAGAA ATTAACTAGC 
CATCAGATGC TTAGTAGCAC AGAAATTCTC AAATGGTTGA GGGAGATTTT GATCTGCAGG 
AATAAATTTC TTCTTAAAAA TAAGCAGGCC GATAGAAGTT CCTGTCACTT TCTCTTCCTT 
TATGGGGTAG GATGTGATGT TCCTTCAAGT GGAACTACTA GTCAGATGTC CATGGATCAT 
GAAGAATTAC TACGTTCTTG TACTCCTGGA GCCTCTCTCC GGAAGGGAAA AGGAAACTCA 
TCCATGGATA GTGCAGCAGG GTGCAGCGGT ACTCCGCCAA TATGCCGACA AGCTCAGACT 
AAACTAGAAG TGGCCCTGTA CATGTTTCTG TGGAGCCCTG ACACTGAAGC TGTTCTGGTT 
GCCATGTCCT GTTTCCGTCA CCTTTGTGAG GAAGCAGATA TCCGGTGTGG GGTGGACGAA 
GTGTCGGTGC ACAACTTCTT GCCCAACTAT AACACATTTA TGGAATTTGC CTCTGTCAGC 
AATATGATGT CAACAGGAAG AGCAGCACTT CAGAAAAGAG TGATGGCACT ACTAAGGCGC 
ATTGAGCATC CCACTGCAGG AAATACCGAG GCTTGGGAAG ATACACATGC GAAGTGGGAA 
CAAGCTACAA AACTAATCCT CAACTACCCA AAAGCTAAAA TGGAAGATGG CCAGGCTGCT 
GAAAGCCTTC ACAAGACCAT TGTTAAGAGG CGAATGTCTC ATGTCAGTGG AGGAGGATCC 
ATAGATTTGT CTGACACGGA CTCCCTACAG GAATGGATCA ACATGACTGG CTTCCTCTGT 
GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA AGCAATTCTG GCTTGGCAAC CTATAGCCCA 
CCCATGGGCC CAGTCAGTGA ACGTAAGGGT TCCATGATTT CAGTGATGTC TTCAGAGGGA 
AATGCAGATA CACCTGTCAG CAAATTTATG GATCGGCTGT TATCCTTAAT GGTGTGTAAC 
CATGAGAAAG TGGGACTTCA AATACGGACC AATGTTAAGG ATCTGGTGGG TCTAGAATTG 
AGTCCTGCTC TTTATCCAAT GCTATTTAAC AAATTGAAGA ATACCATCAG CAAGTTTTTT 
GACTCCCAAG GACAGGTTTT ATTGACTGAC ACCAATACTC AGTTTGTGGA GCAAACCATA 
GCTATAATGA AGAACTTACT AGATAATCAT ACTGAAGGCA GCTCTGAACA CCTGGGGCAA 
GCTAGCATTG AAACAATGAT GTTAAATCTA GTCAGATATG TTCGTGTGCT TGGGAATATG 
GTCCATGCAA TTCAAATAAA AACGAAACTC TGTCAGTTAG TCGAGGTGAT GATGGCGAGG 
AGAGATGACC TCTCATTTTG TCAAGAGATG AAATTTAGGA ATAAGATGGT AGAATACCTG 
ACAGACTGGG TTATGGGAAC ATCCAACCAG GCAGCAGATG ATGATGTAAA ATGTCTTACA 
AGAGACTTGG ACCAGGCAAG CATGGAAGCA GTAGTTTCAC TCCTGGCTGG TCTTCCACTG 
CAGCCGGAGG AAGGAGATGG TGTGGAATTG ATGGAAGCCA AGTCACAGTT ATTTCTTAAG 
TATTTCACAT TGTTCATGAA CCTATTGAAT GACTGTAGTG AAGTTGAAGA TGAAAATGCA 
CAAACAGGTG GCAGGAAACG TGGCATGTCT CGGAGGCTGG CATCACTGAG GCACTGTACA 
GTCCTTGCAA TGTCAAACTT ACTCAATGCT AATGTGGACA GTGGCCTCAT GCATTCCATA 
GGTTTAGGTT ATCACAAAGA TCTTCAGACA AGAGCTACAT TTATGGAAGT TCTGACAAAA 
ATCCTCCAAC AAGGCACAGA ATTTGACACA CTTGCGGAAA CAGTGCTGGC CGACCGGTTT 
GAGAGGCTGG TGGAGTTGGT GACGATGATG GGAGACCAGG GAGAGCTCCC AATAGCCATG 
GCTCTGGCCA ACGTGGTCCC GTGTTCCCAG TGGGATGAAC TAGCTCGAGT TTTGGTGACT 
CTGTTTGATT CTCGGCATTT ACTCTACCAA TTGCTCTGGA ACATGTTTTC TAAGGAAGTA 
GAATTGGCAG ACTCCATGCA GACTCTTTTC CGAGGAAACA GCTTAGCCAG TAAAATAATG 
ACCTTCTGTT TCAAGGTATA TGGTGCTACC TACCTACAAA AACTCTTGGA TCCGTTATTA 
CGAATTGTGA TCACATCCTC TGATTGGCAG CATGTTAGCT TTGAAGTGGA TCCTACCAGA 
TTAGAACAGT CAGAGAGCCT TGAGGAAAAC CAACGGAACC TTCTTCAGAT GACTGAAAAG 
TTCTTCCATG CCATCATCAG TTCCTCCTCA GAATTCCCCC CTCAGCTTCG AAGTGTGTGC 
CACTGTTTAT ACCAGGCAAC TTGCCACTCC CTACTGAATA AAGCTACAGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT GGTTAGCCAG CGTTTCCCTC AGAACAGCAT CGGTGCAGTG 
GGAAGTGCCA TGTTCCTCAG ATTTATCAAT CCTGCCATTG TCTCACCGTA CGAAGCAGGG 
ATTTTAGATA AAAAGCCACC ACCTAGAATT GAAAGGGGCT TGAAGTTAAT GTCAAAGATA 
CTTCAGAGTA TTGCCAATCA TGTTCTTTTC ACAAAAGAAG AGCATATGCG GCCTTTTAAT 
GATTTTGTGA AGAGCAACTT TGATGCAGCA CGGAGGTTTT TCCTTGATAT AGCATCTGAT 
TGTCCTACAA GTGATACAGT AAATCATAGT CTTTCCTTCA TCAGTGATGG CAATGTGCTT 
GCTTTACATC GTCTACTCTG GAACAATCAA GAGAAAATTG GCCAGTATCT TTCCAGCAAC 
AGGGATCACA AAGCTGTTGG AAGACGACCT TTCGATAAGA TGGCAACACT TCTTGCATAC 
CTGGGTCCTC CAGAGCACAA ACCTGTGGCA GATACACACT GGTCCAGCCT TAACCTTACC 
AGTTCAAAGT TTGAGGAATT TATGACTAGG CATCAAGTAC ATGAAAAAGA AGAGTTCAAG 
GCTTTGAAAA CATTAAGTAT TTTCTACCAA GCAGGGACTT CCAAAGCTGG GAATCCTATA 
TTTTATTATG TTGCACGGAG GTTTAAAACT GGTCAAATCA ATGGCGATTT GCTGATATAC 
CATGTCTTGC TGACTTTAAA GCCATATTAT GCAAAGCCAT ATGAAATTGT AGTGGACCTT 
ACGCATACTG GGCCGAGCAA TCGCTTTAAA ACAGACTTTC TCTCCAAGTG GTTTGTTGTT 
TTTCCTGGCT TCGCTTATGA CAATGTCTCT GCAGTCTATA TCTATAACTG TAACTCCTGG 
GTCAGAGAGT ACACCAAGTA TCACGAGCGG CTGCTGACTG GCCTCAAAGG CAGCAAAAGG 
CTCATCTTCA TAGACTGTCC TGGGAAACTG GCTGAGCACA TAGAGCATGA ACAACAGAAA 
CTGCCTGCTG CCACACTGGC TTTAGAAGAG GACCTGAAGG TATTCCACAA TGCTCTCAAG 
CTAGCTCACA AAGACACCAA GGTTTCCATT AAAGTTGGTT CTACTGCTGT TCAAGTAACC 
TCAGCAGAGC GAACAAAAGT CCTGGGGCAA TCGGTCTTTC TAAATGATAT TTATTATGCT 
TCGGAAATTG AAGAGATCTG CCTGGTAGAT GAGAACCAGT TTACCTTAAC CATTGCAAAC 
CAGGGCACAC CACTCACCTT CATGCACCAG GAGTGTGAAG CCATTGTTCA GTCTATCATC 
CACATCCGGA CACGCTGGGA GCTGTCACAA CCTGACTCCA TCCCCCAACA CACCAAGATT 
CGGCCAAAAG ATGTCCCTGG GACGCTGCTC AATATTGCGT TACTTAATTT AGGCAGTTCA 
GACCCTAGTT TACGGTCAGC TGCCTATAAT CTTCTGTGTG CCTTAACTTG TACCTTCAAT 
TTAAAAATCG AGGGCCAATT ACTAGAGACA TCAGGTTTAT GCATCCCTGC CAACAACACC 
CTCTTTATTG TCTCTATTAG TAAGACACTG GCAGCCAATG AGCCACATCT GACCTTAGAA 
TTTTTGGAAG AGTGTATTTC TGGATTTAGC AAGTCTAGTA TTGAATTGAA ACACCTTTGT 
TTGGAATACA TGACTCCGTG GCTGTCAAAT CTAGTCCGTT TTTGTAAGCA CAATGATGAT 
GCCAAAAGAC AGAGAGTTAC TGCTATTCTT GATAAGCTGA TAACAATGAC CATAAATGAG 
AAACAGATGT ACCCATCTAT CCAGGCCAAA ATATGGGGGA GCCTTGGGCA GATTACAGAT 
CTCCTTGATG TTGTATTAGA CAGTTTCATC AAAACCAGTG CAACAGGTGG CTTGGGATCA 
ATAAAAGCTG AGGTGATGGC AGATACTGCT GTAGCTTTGG CTTCTGGAAA CGTGAAATTG 
GTTTCAAGCA AGGTTATTGG AAGGATGTGC AAAATAATTG ACAAGACATG TTTATCTCCA 
ACTCCTACCT TAGAGCAACA TCTTATGTGG GACGATATTG CTATTCTAGC ACGCTACATG 
CTGATGCTGT CCTTCAACAA TTCCCTTGAT GTGGCAGCTC ATCTCCCCTA CCTCTTCCAT 
GTTGTTACTT TCTTAGTAGC TACAGGGCCA CTCTCCCTTA GAGCTTCCAC ACATGGATTG 
GTCATTAATA TCATTCACTC TCTGTGTACT TGTTCACAGC TTCATTTTAG TGAAGAGACA 
AAGCAAGTTT TGAGACTCAG TTTGACAGAG TTCTCATTAC CCAAGTTTTA CCTGCTGTTT 
GGCATTAGTA AAGTCAAGTC AGCTGCCGTC ATTGCCTTTC GCTCCAGTTA CCGGGACAGG 
TCATTCTCTC CTGGCTCCTA TGAAAGGGAG ACTTTTGCTT TGACATCCTT GGAAACAGTC 
ACAGAAGCTT TGTTGGAGAT TATGGAGGCA TGTATGAGAG ATATTCCAAC ATGCAAGTGG 
CTGGACCAGT GGACAGAACT AGCTCAAAGA TTTGCATTCC AATATAATCC ATCCCTGCAA 
CCAAGAGCTC TTGTTGTCTT TGGCTGTATT AGCAAACGAG TGTCTCATGG GCAGATAAAA 
CAGATTATCC GTATTCTTAG CAAGGCACTT GAGAGTTGCT TAAAAGGACC TGATACTTAC 
AACAGTCAAG TTCTGATAGA AGCTACAGTA ATAGCACTAA CCAAATTACA ACCACTTCTT 
AATAAGGACT CCCCTCTGCA CAAAGCCCTC TTTTGGGTGG CTGTGGCTGT GCTACAGCTC 
GATGAGGTCA ACCTGTATTC AGCAGGCAGT GCGCTTCTGG AACAAAACCT GCACACCTTA 
GACAGTCTGC GGGTATTCAA TGACAAGAGT CCAGAGGAAG TATTTATGGC AATCCGGAAT 
CCTCTGGAAT GGCACTGCAA ACAAATGGAT CATTTTGTTG GACTCAATTT CAACTCTAAC 
TTTAACTTCG CACTGGTTGG ACACCTCTTA AAAGGGTACA GGCATCCTTC ACCTGCCATT 
GTGGCAAGAA CAGTCAGAAT TTTGCATACA CTACTAACTC TGGTGAACAA ACATAGAAAC 
TGTGACAAAT TTGAGGTGAA CACACAGAGC GTGGCTTATT TAGCAGCCTT ACTCACAGTG 
TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA AAACATAGAA AATCTCTTCT TCTTACTGAT 
ATTTCAATGG AAAATGTTCC TATGGATACG TATCCCATCC ATCATGGCGA CCCTAGCTAT 
AGGACGCTAA AGGAGAACCA GCCGTGGTCA TCTCCCAAAG GTTCTGAAGG GTACCTTGCA 
GCCTCCTATC CAGCCGTGGG CCAGACCAGT CCCCGAGCCA GGAAATCCAT GAGTTTGGAC 
ATGGGGCAGC CTTCTCAGGC CAATACGAAG AAGTTGCTGG GAACAAGGAA AAGTTTTGAT 
CACTTGATAT CAGACACAAA GGCTCCTAAA AGGCAAGAAA TGGAATCAGG GATCACAACA 
CCCCCCAAAA TGAGGAGAGT AGCAGAAACT GATTATGAAA TGGAAACTCA GAGGATTTCC 
TCATCACAGC AACACCCACA TTTACGCAAA GTTTCAGTGT CTGAATCAAA TGTTCTCTTG 
GATGAAGAAG TACTTACTGA TCCGAAGATC CAAGCACTTC TTCTTACTGT TCTGGCTACA 
CTGGTAAAAT ACACCACAGA TGAGTTTGAT CAGCGAATTC TTTATGAATA CTTAGCAGAA 
GCCAGTGTTG TTTTCCCCAA GGTTTTTCCT GTTGTGCACA ATTTGTTGGA CTCTAAGATC 
AACACCTTGT TGTCATTGTG CCAAGATCCA AATTTGTTAA ATCCAATCCA TGGAATCGTG 
CAGAGTGTGG TATACCATGA AGAATCCCCA CCTCAGTACC AAACATCTTA TCTGCAAAGT 
TTTGGTTTTA ATGGCTTATG GCGGTTTGCA GGACCCTTTT CAAAGCAAAC ACAAATCCCA 
GACTATGCTG AGCTTATTGT TAAGTTTCTT GATGCCTTGA TTGACACGTA TTTGCCTGGA 
ATTGATGAAG AAACCAGTGA GGAGTCCCTC CTGACACCCA CTTCTCCTTA TCCTCCTGCA 
GTGCAGAGCC AGCTTAGTAT TACTGCTAAT CTTAACCTTT CTAATTCCAT GACCTCACTT 
GCAACTTCCC AGCATTCCCC AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCAACCACT 
GGGCACTGTA ACAGTGGACG GACTCGCCAT GGATCCGCAA GCCAAGTGCA GAAGCAAAGA 
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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