POLA1 cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe32205 XM_005641214.3
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Canis lupus familiaris DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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OCe32205 XM_005641214.2 Canis lupus familiaris polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe32205
    Clone ID Related Accession (Same CDS sequence) XM_005641214.3 , XM_005641214.2
    Accession Version XM_005641214.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA polymerase alpha catalytic subunit
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT
    CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG
    GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA
    GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC
    AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT
    CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG
    GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA
    GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC
    CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG
    TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG
    ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG
    AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG
    AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG
    GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT
    CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA
    CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC
    CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC
    CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA
    GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC
    AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG
    ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA
    TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT
    GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT
    AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG
    TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC
    AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT
    CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG
    AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT
    TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG
    TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG
    AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT
    GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA
    CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG
    ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT
    GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG
    GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT
    GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT
    TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA
    AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA
    GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC
    ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC
    GCAGACTGTG CTGTGAGGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641271.1
    CDS115..4518
    Translation

    Target ORF information:

    RefSeq Version XM_005641214.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641214.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT
    CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG
    GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA
    GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC
    AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT
    CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG
    GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA
    GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC
    CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG
    TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG
    ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG
    AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG
    AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG
    GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT
    CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA
    CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC
    CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC
    CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA
    GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC
    AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG
    ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA
    TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT
    GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT
    AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG
    TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC
    AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT
    CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG
    AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT
    TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG
    TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG
    AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT
    GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA
    CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG
    ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT
    GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG
    GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT
    GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT
    TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA
    AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA
    GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC
    ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC
    GCAGACTGTG CTGTGAGGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32205
    Clone ID Related Accession (Same CDS sequence) XM_005641214.3 , XM_005641214.2
    Accession Version XM_005641214.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA polymerase alpha catalytic subunit
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT
    CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG
    GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA
    GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC
    AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT
    CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG
    GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA
    GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC
    CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG
    TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG
    ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG
    AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG
    AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG
    GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT
    CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA
    CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC
    CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC
    CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA
    GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC
    AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG
    ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA
    TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT
    GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT
    AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG
    TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC
    AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT
    CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG
    AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT
    TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG
    TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG
    AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT
    GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA
    CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG
    ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT
    GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG
    GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT
    GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT
    TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA
    AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA
    GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC
    ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC
    GCAGACTGTG CTGTGAGGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641271.1
    CDS178..4581
    Translation

    Target ORF information:

    RefSeq Version XM_005641214.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641214.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT
    CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC
    ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG
    GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA
    GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC
    AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC
    AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT
    CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG
    GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA
    GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC
    CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG
    TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG
    ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG
    AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG
    AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG
    GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT
    CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA
    CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC
    CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC
    CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA
    GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC
    AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG
    ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA
    TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT
    GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT
    AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG
    TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC
    AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT
    CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG
    AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT
    TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG
    TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG
    AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT
    GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA
    CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG
    ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT
    GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG
    GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT
    GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT
    TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA
    AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA
    GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC
    ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC
    GCAGACTGTG CTGTGAGGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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