PTCH2 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following PTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe17389 XM_005629082.3
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe293508 XM_022428023.1
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293509 XM_022428025.1
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe40354 XM_014119704.2
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe17386 XM_005629080.3
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17387 XM_539636.7
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17388 XM_005629081.3
Latest version!
Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe17386 XM_005629080.2 Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17389 XM_005629082.2 Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe40354 XM_014119704.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe17387 XM_539636.6 Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17388 XM_005629081.2 Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe17389
    Clone ID Related Accession (Same CDS sequence) XM_005629082.3 , XM_005629082.2
    Accession Version XM_005629082.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT
    GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT
    CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT
    CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629139.1
    CDS121..3117
    Translation

    Target ORF information:

    RefSeq Version XM_005629082.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629082.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT
    GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT
    CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT
    CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17389
    Clone ID Related Accession (Same CDS sequence) XM_005629082.3 , XM_005629082.2
    Accession Version XM_005629082.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein patched homolog 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT
    GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT
    CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT
    CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629139.1
    CDS363..3359
    Translation

    Target ORF information:

    RefSeq Version XM_005629082.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT
    GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT
    CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT
    CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293508
    Clone ID Related Accession (Same CDS sequence) XM_022428023.1
    Accession Version XM_022428023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGCGCTTCA
    GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC TGGCTACACT
    ATTACCGGAA CTGGCTCCTG GGTGAGAGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283731.1
    CDS122..3742
    Translation

    Target ORF information:

    RefSeq Version XM_022428023.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022428023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGCGCTTCA
    GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC TGGCTACACT
    ATTACCGGAA CTGGCTCCTG GGTGAGAGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293509
    Clone ID Related Accession (Same CDS sequence) XM_022428025.1
    Accession Version XM_022428025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGATAC AGACCCCACG CCAGGAAGGG GAGAACGTCC TCACCCCCGA GGCGCTCGGC 
    CTCCACCTCC AGGCAGCCCT CACCGCCAGT AAAGTGCAAG TATCACTCTA CGGAAAGTCC
    TGGGATTTGA ACAAAATCTG CTACAAGTCA GGGATTCCCC TAATTGAAAA TGGAATGATT
    GAGCGGATGA TAGAGAAGCT GTTTCCGTGC GTGATCCTCA CCCCCCTCGA CTGCTTCTGG
    GAGGGCGCCA AGCTCCAAGG GGGCTCAGCC TACTTGCCTG GCCGCCCTGA CATCCAGTGG
    ACCAACCTGG ATCCAGAGCA GCTGCTGGAG GAGCTGGGGC CCTTTGCCTC CCTCGAGGGC
    TTCCGGGAGC TGCTAGACAA GGCACAGGTG GGCCAGGCCT ATGTGGGGCG GCCCTGTCTG
    CACCCTGACG ACCTCCACTG CCCGCCTAGT GCCCCTAACC ATCACAGCAA GCAGGCTCCC
    AACGTGGCTC AGGAGTTGAG TGGGGGCTGT CACGGCTTTT CCCACAAGTT CATGCACTGG
    CAGGAGGAAC TGCTGCTGGG GGGCATGGCC AGAGACCCCC AAGGACAGCT GCTGAGGGCG
    GAGGCCCTGC AGAGCACCTT CCTGCTGATG AGTCCCCGCC AGCTGTACGA GCACTTCCGG
    GGCGACTACC AGACGCATGA CATCGGCTGG AGCGAGGAGC AGGCGGGGAC AGTGCTACAG
    GCCTGGCAGC GGCGCTTCGT GCAGGTCAGT GTGCGGATGG GCAAGGGCGG TGCCCGAGGC
    TGCTCCCTCC TCCTGCCCCT CAGATCCACC CTGTCTCTGC AGCTGGCTCA GGAGGCCCTG
    CCTCAGAATT CATCCCAGCA GATCCATGCC TTTTCCTCCA CCACTCTGGA TGACATCCTG
    CATGCTTTCT CTGAAGTCAG TGCTGCCCGT GTGGTCGGAG GCTATCTGCT CATGCTAGCC
    TATGCTTGCG TGACGATGCT GCGCTGGGAC TGTGCCCAGT CCCAGGGTGC CGTGGGCCTT
    GCGGGGGTGC TGCTGGTAGC CCTGGCGGTG GCCTCGGGCC TTGGGCTCTG CGCCCTGCTG
    GGCATCGCCT TCAATGCCGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACATGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CAGGTGAGTG TCTGCAGCGC ACGGGCACCA GCGTCGCACT CACGTCCATC
    AGCCACATGG TTGCCTTCTT CATGGCCGCC CTGGTTCCCA TCCCGGCCCT GCGGGCCTTC
    TCCCTGCAGG CAGCCATAGT CGTTGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GTCTGGACCT GCACCGACGC CACTGCCAGC GCCTTGACGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC GCGGGTAATT CAGATCCTGC CCCAGGAACT GGGGGACGGG
    ACGGTGCCAG TGGGCATCGC CCACCTGACC GCCACGGTTC AAGCCTTTGC CCACTGCGAA
    GCCGGCAGCC AGCACGTCGT CACCATCCTG CCTCCCCGAG CCCGCTTGGT GCCCCCACCT
    TCTGACCCAC TGGGCTCTGA GCTCTTCAGC CCAGGAGGAT CCACACGGGA CCTTCTAGGC
    CAAGAGGAGG GGACGAGACA AAAGGCAACC TGCAGCTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TTGCCCGCTC TCAGTTTGCA CCCTTGCTGC TCCAGTCACA CAGCAAGGCC
    ATCGTGCTGG TGCTCTTTGG GGCTCTCCTG GGCCTGAGCC TCTACGGAGC AACCTTGGTG
    CAGGATGGGC TGGCCCTGAC GGATGTGGTG CCTCGGGGCA CCAAGGAGCA CGCCTTCCTG
    AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCCC TGGTGACGCA GGGTGGCTTT
    GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCTCTCAAG
    GCCGTGCTGC CCACACCTGC CACCCAGGCG CCGCGCACCT GGCTACACTA TTACCGGAAC
    TGGCTCCTGG GAATCCAGGC CGCCTTTGAC CAGGACTGGG CTTCTGGGCG CATTAGCCGC
    CACTCGTGCC GCAATGGCTC CGAGGACGGG GCCCTGGCCT ACAAGCTGCT CCTCCAGACC
    GGGGATGCCC AAGAGCCTCT GGATTTCAGC CAGCTGACCA CACGGAAGCT GGTAGACAAG
    GAGGGGCTGA TTGCACCTGA GCTCTTCTAC GTGGGGCTGA CCATGTGGGT AAGCAGTGAC
    CCGCTGGGCC TGGCGGCCTC ACAGGCCAAC TTCTACCCCC CACCTCCCGA GTGGCTGCAC
    GACAAGTACG ACACCACCGG GGAGAACCTT CGCATCCCGG CGGCCCAGCC CCTGGAGTTT
    GCCCAGTTCC CCTTCCTACT GCGTGGCCTC CAGAAGACTG CGGACTTTGT GGAGGCCATC
    GAGGGGGCCC GGGCAGCATG CGCCGAGGCA GGTCAGGCCG GGGTGCGCGC CTACCCCAGC
    GGCTCCCCCT TCCTCTTCTG GGAGCAGTAT CTGGGCCTGC GGCGCTACTT CCTGCTGGCT
    ATCTGCATCC TGCTGGTATG CACTTTCCTC GTCTGTGCCC TGCTGCTGCT CAACCCCTGG
    ACGGCTGGCC TCATCGTACT GGTCCTGGCG ATGATGACTG TGGAGCTCTT TGGCATCATG
    GGTTTCCTGG GCATCAAACT GAGTGCCATC CCCGTGGTGA TCCTTGTGGC CTCTGTGGGC
    ATTGGTGTTG AGTTCACAGT CCACGTGGCT CTGGGCTTCC TGACCACCCA GGGTAGCCGG
    AACCTGCGGG CTGCCCACGC CCTAGAGCGC ACGTTTGCCC CAGTGACCGA TGGGGCCGTG
    TCCACGTTGC TGGGTCTGCT CATGCTTGCT GGCTCCAACT TTGACTTCAT CATACGGTAC
    TTTTTCGTGG TGCTAACTGT GCTCACACTC CTGGGCCTCC TCCATGGGCT CGTGCTGCTG
    CCTGTGCTGC TGTCCATCCT GGGCCCCCCG CCAGAGGTGG TACAGATGTA CAAGGAGAGC
    CCAGAGGTCC TCAGCCCCCC AGCTCCACGA GAAGGAGGGC TCAGGTGGGG GTTGCCCCCC
    ACCCTGCCCC AGAGCTTTGC CAGAGTGACT ACCTCCATGA CGGTGGCCCT CCACCCACCC
    CCACTGCCCG GTGCCTACAT CCACCCAGCC TCTGACGAGC CCACTTGGTC CCCTGTTGCC
    ACATCAGCTG CCAGCGGCTC CAGCAACCTC AGTTCCAGGG GACCATGTCC AGCCACTGGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283733.1
    CDS226..3588
    Translation

    Target ORF information:

    RefSeq Version XM_022428025.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022428025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGATAC AGACCCCACG CCAGGAAGGG GAGAACGTCC TCACCCCCGA GGCGCTCGGC 
    CTCCACCTCC AGGCAGCCCT CACCGCCAGT AAAGTGCAAG TATCACTCTA CGGAAAGTCC
    TGGGATTTGA ACAAAATCTG CTACAAGTCA GGGATTCCCC TAATTGAAAA TGGAATGATT
    GAGCGGATGA TAGAGAAGCT GTTTCCGTGC GTGATCCTCA CCCCCCTCGA CTGCTTCTGG
    GAGGGCGCCA AGCTCCAAGG GGGCTCAGCC TACTTGCCTG GCCGCCCTGA CATCCAGTGG
    ACCAACCTGG ATCCAGAGCA GCTGCTGGAG GAGCTGGGGC CCTTTGCCTC CCTCGAGGGC
    TTCCGGGAGC TGCTAGACAA GGCACAGGTG GGCCAGGCCT ATGTGGGGCG GCCCTGTCTG
    CACCCTGACG ACCTCCACTG CCCGCCTAGT GCCCCTAACC ATCACAGCAA GCAGGCTCCC
    AACGTGGCTC AGGAGTTGAG TGGGGGCTGT CACGGCTTTT CCCACAAGTT CATGCACTGG
    CAGGAGGAAC TGCTGCTGGG GGGCATGGCC AGAGACCCCC AAGGACAGCT GCTGAGGGCG
    GAGGCCCTGC AGAGCACCTT CCTGCTGATG AGTCCCCGCC AGCTGTACGA GCACTTCCGG
    GGCGACTACC AGACGCATGA CATCGGCTGG AGCGAGGAGC AGGCGGGGAC AGTGCTACAG
    GCCTGGCAGC GGCGCTTCGT GCAGGTCAGT GTGCGGATGG GCAAGGGCGG TGCCCGAGGC
    TGCTCCCTCC TCCTGCCCCT CAGATCCACC CTGTCTCTGC AGCTGGCTCA GGAGGCCCTG
    CCTCAGAATT CATCCCAGCA GATCCATGCC TTTTCCTCCA CCACTCTGGA TGACATCCTG
    CATGCTTTCT CTGAAGTCAG TGCTGCCCGT GTGGTCGGAG GCTATCTGCT CATGCTAGCC
    TATGCTTGCG TGACGATGCT GCGCTGGGAC TGTGCCCAGT CCCAGGGTGC CGTGGGCCTT
    GCGGGGGTGC TGCTGGTAGC CCTGGCGGTG GCCTCGGGCC TTGGGCTCTG CGCCCTGCTG
    GGCATCGCCT TCAATGCCGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGC
    GTGGATGACA TATTCCTGCT GGCACATGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC
    CAGGAGCGCA CAGGTGAGTG TCTGCAGCGC ACGGGCACCA GCGTCGCACT CACGTCCATC
    AGCCACATGG TTGCCTTCTT CATGGCCGCC CTGGTTCCCA TCCCGGCCCT GCGGGCCTTC
    TCCCTGCAGG CAGCCATAGT CGTTGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA
    GCGGTCCTCA GTCTGGACCT GCACCGACGC CACTGCCAGC GCCTTGACGT GCTCTGCTGC
    TTCTCTAGCC CCTGCTCTGC GCGGGTAATT CAGATCCTGC CCCAGGAACT GGGGGACGGG
    ACGGTGCCAG TGGGCATCGC CCACCTGACC GCCACGGTTC AAGCCTTTGC CCACTGCGAA
    GCCGGCAGCC AGCACGTCGT CACCATCCTG CCTCCCCGAG CCCGCTTGGT GCCCCCACCT
    TCTGACCCAC TGGGCTCTGA GCTCTTCAGC CCAGGAGGAT CCACACGGGA CCTTCTAGGC
    CAAGAGGAGG GGACGAGACA AAAGGCAACC TGCAGCTCCC TGCCCTGTGC CCGCTGGAAT
    CTTGCCCATT TTGCCCGCTC TCAGTTTGCA CCCTTGCTGC TCCAGTCACA CAGCAAGGCC
    ATCGTGCTGG TGCTCTTTGG GGCTCTCCTG GGCCTGAGCC TCTACGGAGC AACCTTGGTG
    CAGGATGGGC TGGCCCTGAC GGATGTGGTG CCTCGGGGCA CCAAGGAGCA CGCCTTCCTG
    AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCCC TGGTGACGCA GGGTGGCTTT
    GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCTCTCAAG
    GCCGTGCTGC CCACACCTGC CACCCAGGCG CCGCGCACCT GGCTACACTA TTACCGGAAC
    TGGCTCCTGG GAATCCAGGC CGCCTTTGAC CAGGACTGGG CTTCTGGGCG CATTAGCCGC
    CACTCGTGCC GCAATGGCTC CGAGGACGGG GCCCTGGCCT ACAAGCTGCT CCTCCAGACC
    GGGGATGCCC AAGAGCCTCT GGATTTCAGC CAGCTGACCA CACGGAAGCT GGTAGACAAG
    GAGGGGCTGA TTGCACCTGA GCTCTTCTAC GTGGGGCTGA CCATGTGGGT AAGCAGTGAC
    CCGCTGGGCC TGGCGGCCTC ACAGGCCAAC TTCTACCCCC CACCTCCCGA GTGGCTGCAC
    GACAAGTACG ACACCACCGG GGAGAACCTT CGCATCCCGG CGGCCCAGCC CCTGGAGTTT
    GCCCAGTTCC CCTTCCTACT GCGTGGCCTC CAGAAGACTG CGGACTTTGT GGAGGCCATC
    GAGGGGGCCC GGGCAGCATG CGCCGAGGCA GGTCAGGCCG GGGTGCGCGC CTACCCCAGC
    GGCTCCCCCT TCCTCTTCTG GGAGCAGTAT CTGGGCCTGC GGCGCTACTT CCTGCTGGCT
    ATCTGCATCC TGCTGGTATG CACTTTCCTC GTCTGTGCCC TGCTGCTGCT CAACCCCTGG
    ACGGCTGGCC TCATCGTACT GGTCCTGGCG ATGATGACTG TGGAGCTCTT TGGCATCATG
    GGTTTCCTGG GCATCAAACT GAGTGCCATC CCCGTGGTGA TCCTTGTGGC CTCTGTGGGC
    ATTGGTGTTG AGTTCACAGT CCACGTGGCT CTGGGCTTCC TGACCACCCA GGGTAGCCGG
    AACCTGCGGG CTGCCCACGC CCTAGAGCGC ACGTTTGCCC CAGTGACCGA TGGGGCCGTG
    TCCACGTTGC TGGGTCTGCT CATGCTTGCT GGCTCCAACT TTGACTTCAT CATACGGTAC
    TTTTTCGTGG TGCTAACTGT GCTCACACTC CTGGGCCTCC TCCATGGGCT CGTGCTGCTG
    CCTGTGCTGC TGTCCATCCT GGGCCCCCCG CCAGAGGTGG TACAGATGTA CAAGGAGAGC
    CCAGAGGTCC TCAGCCCCCC AGCTCCACGA GAAGGAGGGC TCAGGTGGGG GTTGCCCCCC
    ACCCTGCCCC AGAGCTTTGC CAGAGTGACT ACCTCCATGA CGGTGGCCCT CCACCCACCC
    CCACTGCCCG GTGCCTACAT CCACCCAGCC TCTGACGAGC CCACTTGGTC CCCTGTTGCC
    ACATCAGCTG CCAGCGGCTC CAGCAACCTC AGTTCCAGGG GACCATGTCC AGCCACTGGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40354
    Clone ID Related Accession (Same CDS sequence) XM_014119704.1 , XM_014119704.2
    Accession Version XM_014119704.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3681bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein patched homolog 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC
    CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC
    TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT
    GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC
    CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG
    CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC
    AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG
    CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC
    ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC
    TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC
    CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT
    GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC
    TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT
    CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC
    GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC
    CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC
    GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC
    CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC
    CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA
    GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC
    CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC
    ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG
    CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC
    CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC
    TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC
    AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG
    GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG
    CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG
    CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975179.1
    CDS362..4042
    Translation

    Target ORF information:

    RefSeq Version XM_014119704.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC
    CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC
    TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT
    GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC
    CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG
    CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC
    AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG
    CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC
    ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC
    TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC
    CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT
    GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC
    TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT
    CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC
    GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC
    CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC
    GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC
    CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC
    CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA
    GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC
    CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC
    ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG
    CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC
    CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC
    TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC
    AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG
    GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG
    CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG
    CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40354
    Clone ID Related Accession (Same CDS sequence) XM_014119704.1 , XM_014119704.2
    Accession Version XM_014119704.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3681bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC
    CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC
    TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT
    GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC
    CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG
    CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC
    AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG
    CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC
    ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC
    TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC
    CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT
    GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC
    TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT
    CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC
    GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC
    CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC
    GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC
    CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC
    CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA
    GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC
    CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC
    ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG
    CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC
    CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC
    TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC
    AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG
    GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG
    CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG
    CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975179.1
    CDS121..3801
    Translation

    Target ORF information:

    RefSeq Version XM_014119704.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119704.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA
    ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC
    CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC
    CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC
    TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT
    GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT
    CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC
    CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA
    GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG
    CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG
    GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC
    AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG
    CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC
    ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC
    TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC
    CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT
    GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC
    TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT
    CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC
    GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC
    CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC
    GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC
    CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC
    CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA
    GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC
    CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC
    ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG
    CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC
    CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC
    TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC
    AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG
    GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG
    CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG
    CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT
    TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC
    AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA
    CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC
    ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA
    CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG
    GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG
    GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG
    GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG
    CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG
    CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG
    GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC
    CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG
    ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA
    GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT
    GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC
    CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA
    CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC
    AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG
    GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC
    ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA
    CCATGTCCAG CCACTGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17386
    Clone ID Related Accession (Same CDS sequence) XM_005629080.2 , XM_005629080.3
    Accession Version XM_005629080.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein patched homolog 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA
    GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC
    AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC
    CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC
    AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629137.1
    CDS362..4063
    Translation

    Target ORF information:

    RefSeq Version XM_005629080.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA
    GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC
    AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC
    CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC
    AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17386
    Clone ID Related Accession (Same CDS sequence) XM_005629080.2 , XM_005629080.3
    Accession Version XM_005629080.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA
    GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC
    AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC
    CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC
    AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629137.1
    CDS120..3821
    Translation

    Target ORF information:

    RefSeq Version XM_005629080.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629080.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA
    GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC
    AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC
    CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC
    AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17387
    Clone ID Related Accession (Same CDS sequence) XM_539636.7 , XM_539636.6
    Accession Version XM_539636.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539636.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC
    ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA
    GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG
    TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC
    CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC
    AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC
    ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG
    CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG
    CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT
    CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA
    GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA
    TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG
    CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC
    ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC
    CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC
    TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG
    GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC
    TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC
    ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG
    ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC
    CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG
    GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT
    GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC
    GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG
    CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG
    ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC
    CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC
    CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC
    CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG
    GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG
    CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG
    CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG
    GGACCATGTC CAGCCACTGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539636.3
    CDS1..3624
    Translation

    Target ORF information:

    RefSeq Version XM_539636.7
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539636.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC
    ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA
    GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG
    TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC
    CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC
    AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC
    ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG
    CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG
    CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT
    CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA
    GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA
    TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG
    CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC
    ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC
    CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC
    TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG
    GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC
    TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC
    ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG
    ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC
    CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG
    GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT
    GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC
    GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG
    CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG
    ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC
    CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC
    CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC
    CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG
    GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG
    CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG
    CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG
    GGACCATGTC CAGCCACTGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17387
    Clone ID Related Accession (Same CDS sequence) XM_539636.7 , XM_539636.6
    Accession Version XM_539636.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein patched homolog 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539636.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC
    ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA
    GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG
    TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC
    CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC
    AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC
    ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG
    CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG
    CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT
    CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA
    GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA
    TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG
    CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC
    ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC
    CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC
    TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG
    GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC
    TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC
    ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG
    ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC
    CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG
    GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT
    GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC
    GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG
    CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG
    ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC
    CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC
    CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC
    CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG
    GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG
    CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG
    CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG
    GGACCATGTC CAGCCACTGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539636.3
    CDS361..3984
    Translation

    Target ORF information:

    RefSeq Version XM_539636.6
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539636.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC
    ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA
    GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG
    TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC
    CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC
    TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG
    GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC
    AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC
    ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA
    GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG
    CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG
    CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT
    CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA
    GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA
    TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC
    CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG
    CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC
    CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC
    ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC
    CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC
    CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC
    TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG
    GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC
    TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC
    ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG
    ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC
    CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG
    GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT
    GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC
    GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG
    CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC
    CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT
    GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG
    ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC
    CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC
    CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC
    TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC
    CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG
    GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG
    CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG
    ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG
    CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG
    GGACCATGTC CAGCCACTGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17388
    Clone ID Related Accession (Same CDS sequence) XM_005629081.3 , XM_005629081.2
    Accession Version XM_005629081.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein patched homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629138.1
    CDS121..3594
    Translation

    Target ORF information:

    RefSeq Version XM_005629081.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629081.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17388
    Clone ID Related Accession (Same CDS sequence) XM_005629081.3 , XM_005629081.2
    Accession Version XM_005629081.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein patched homolog 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629138.1
    CDS362..3835
    Translation

    Target ORF information:

    RefSeq Version XM_005629081.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
    ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC
    CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC
    CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA
    GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC
    AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC
    CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC
    ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC
    TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG
    TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG
    GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG
    CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG
    GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC
    CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT
    GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG
    GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC
    CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC
    ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG
    CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC
    AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG
    ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT
    GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG
    CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC
    CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC
    ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG
    GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT
    CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC
    ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC
    GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG
    CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG
    CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC
    CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC
    ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG
    CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA
    AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT
    CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG
    GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG
    GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC
    TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC
    GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC
    ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC
    GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC
    GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG
    GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG
    CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA
    CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG
    GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG
    CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC
    GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG
    GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC
    ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG
    GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG
    AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC
    CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC
    CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC
    ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG
    CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG
    GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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