AMBRA1 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following AMBRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMBRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294797 XM_022405477.1
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe20103 XM_005631289.3
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20103 XM_005631290.2
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20104 XM_005631291.3
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20105 XM_540754.5
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20106 XM_005631292.3
Latest version!
Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe20103 XM_005631289.2 Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20103 XM_005631290.1 Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20104 XM_005631291.2 Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20106 XM_005631292.2 Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20105 XM_540754.4 Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294797
    Clone ID Related Accession (Same CDS sequence) XM_022405477.1
    Accession Version XM_022405477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGGACAATG GTGACAGATC TAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCGCCT TCGCTTGGAC GCTTCGTTCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCGGGG ATTTTTCACG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CCTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCA
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CGGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTAGCAGC TTTCATCCCT
    AGCAGCCAAA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCGTT GGCCCCCCAT
    AACCTGGGTG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTA
    TCGCCAATGG GCCGATACGT AATGGTGGGC TTGGCCTCGA GGAGGATCCT GCTGCATCCC
    TCCACAGAGC ACATGGTGGC CCAAGTCTTC AGGCTGCAAC AGGCCCATGG TGGCGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC
    AGCATCAACT CTGCCCGTTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA TGGTACCAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTAA ATGAGACTGT CTTCACTGTC CACTCCAGCA GCAGGAGCAG TGAAAGGCCT
    GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GTCTGATGAA TGCAATTGGG
    CTGCAGCCCC GGAACCCCAC CACCTCTGTG ACATCTCAGG GCACCCAGAC CCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACGGAG AGGGAAATCC AGGAGCCCGG GACAGCAGCT
    TCGGGTCCTG GTGAAGGCGA AGGCTCAGAG TCCGGTGCGA GCGGGGAGGA CGCCCTCAGC
    AGGATCCAGC GGCTGATGGC GGAAGGGGGC ATGACGGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAGCATCA TCGTCAGCCA CCGCATCCAC
    CGCGGCTCCC AGACGGGCGC TGAGCCAACG GCCGCCCGCG CCTCCTCGCC CCGGCCCTCC
    GCCTCTCGGG GACTGCTCCC GGAGCCTGGG CAGCTGGCGG AGCGGGGCCT GAGCCCCCGG
    ACAGCCTCCT GGGACCAGCC TGGTGCCCCC GGGCGAGAGC CGCCCCAGCC CCCCCTGCCA
    CCTCTGCCCT CTTCCTCCCC TGTCCCTGCC CCTCTCCCCC TTCCTGGCAC CGAAGGACCA
    ACGCTGCACT GCGACCTGAC CAGCAACAAC CACCTTGCTG ATGGCGGTGG CAGCGGCAGG
    GGGGAAGCTG CAGGCCCCAG CAGGGAGCCA CGGAACAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261185.1
    CDS342..3983
    Translation

    Target ORF information:

    RefSeq Version XM_022405477.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022405477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGGACAATG GTGACAGATC TAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCGCCT TCGCTTGGAC GCTTCGTTCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCGGGG ATTTTTCACG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CCTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCA
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CGGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTAGCAGC TTTCATCCCT
    AGCAGCCAAA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCGTT GGCCCCCCAT
    AACCTGGGTG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTA
    TCGCCAATGG GCCGATACGT AATGGTGGGC TTGGCCTCGA GGAGGATCCT GCTGCATCCC
    TCCACAGAGC ACATGGTGGC CCAAGTCTTC AGGCTGCAAC AGGCCCATGG TGGCGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC
    AGCATCAACT CTGCCCGTTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA TGGTACCAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTAA ATGAGACTGT CTTCACTGTC CACTCCAGCA GCAGGAGCAG TGAAAGGCCT
    GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GTCTGATGAA TGCAATTGGG
    CTGCAGCCCC GGAACCCCAC CACCTCTGTG ACATCTCAGG GCACCCAGAC CCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACGGAG AGGGAAATCC AGGAGCCCGG GACAGCAGCT
    TCGGGTCCTG GTGAAGGCGA AGGCTCAGAG TCCGGTGCGA GCGGGGAGGA CGCCCTCAGC
    AGGATCCAGC GGCTGATGGC GGAAGGGGGC ATGACGGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAGCATCA TCGTCAGCCA CCGCATCCAC
    CGCGGCTCCC AGACGGGCGC TGAGCCAACG GCCGCCCGCG CCTCCTCGCC CCGGCCCTCC
    GCCTCTCGGG GACTGCTCCC GGAGCCTGGG CAGCTGGCGG AGCGGGGCCT GAGCCCCCGG
    ACAGCCTCCT GGGACCAGCC TGGTGCCCCC GGGCGAGAGC CGCCCCAGCC CCCCCTGCCA
    CCTCTGCCCT CTTCCTCCCC TGTCCCTGCC CCTCTCCCCC TTCCTGGCAC CGAAGGACCA
    ACGCTGCACT GCGACCTGAC CAGCAACAAC CACCTTGCTG ATGGCGGTGG CAGCGGCAGG
    GGGGAAGCTG CAGGCCCCAG CAGGGAGCCA CGGAACAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20103
    Clone ID Related Accession (Same CDS sequence) XM_005631290.1 , XM_005631289.3 , XM_005631289.2 , XM_005631290.2
    Accession Version XM_005631290.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631347.1
    CDS182..4087
    Translation

    Target ORF information:

    RefSeq Version XM_005631290.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20103
    Clone ID Related Accession (Same CDS sequence) XM_005631290.1 , XM_005631289.3 , XM_005631289.2 , XM_005631290.2
    Accession Version XM_005631289.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631346.1
    CDS341..4246
    Translation

    Target ORF information:

    RefSeq Version XM_005631289.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631289.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20103
    Clone ID Related Accession (Same CDS sequence) XM_005631290.1 , XM_005631289.3 , XM_005631289.2 , XM_005631290.2
    Accession Version XM_005631289.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631346.1
    CDS770..4675
    Translation

    Target ORF information:

    RefSeq Version XM_005631289.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631289.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20103
    Clone ID Related Accession (Same CDS sequence) XM_005631290.1 , XM_005631289.3 , XM_005631289.2 , XM_005631290.2
    Accession Version XM_005631290.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631347.1
    CDS240..4145
    Translation

    Target ORF information:

    RefSeq Version XM_005631290.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631290.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCAGAGGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTATCAGCAG AACCGTCTCC GTTCTTCCAC CTCCTCTTCC
    TCAGACAACC AGGGCCCATC AGTAGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC
    AATGGTGACA GATCTAGGCA CCGAGCTCCA CGCAATGCCC GGATGTCTGC GCCTTCGCTT
    GGACGCTTCG TTCCAAGGCG TTTCTTGCTG CCTGAGTACT TGCCTTATGC GGGGATTTTT
    CACGAACGTG GACAGCCTGG CTTGGCTACT CACTCCTCTG TTAACAGGGT CCTGGCAGGG
    GCAGTGATTG GTGATGGACA GTCAGCTGTG GCCAGTAACA TTGCCAATAC TACCTACCGG
    CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC TTCCGTGAAT
    GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC
    CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC CAAAGGGGCT TTCCTGATGA AGGCATCCTG
    GCAGTGTACT CGTTGGCCCC CCATAACCTG GGTGAAATGC TCTACACCAA GCGATTTGGT
    CCCAATGCCA TTTCGGTGAG CCTATCGCCA ATGGGCCGAT ACGTAATGGT GGGCTTGGCC
    TCGAGGAGGA TCCTGCTGCA TCCCTCCACA GAGCACATGG TGGCCCAAGT CTTCAGGCTG
    CAACAGGCCC ATGGTGGCGA GACCTCCATG AGGAGAGTTT TCAACGTCCT TTACCCCATG
    CCTGCTGACC AGCGGAGACA TGTCAGCATC AACTCTGCCC GTTGGCTGCC CGAGCCAGGG
    CTTGGCCTGG CCTATGGTAC CAACAAAGGA GACCTGGTGA TCTGCCGACC AGAGGCCTTA
    AACTCTGGTG TTGAGTACTA CTGGGACCAG CTAAATGAGA CTGTCTTCAC TGTCCACTCC
    AGCAGCAGGA GCAGTGAAAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC
    ATGGGTCTGA TGAATGCAAT TGGGCTGCAG CCCCGGAACC CCACCACCTC TGTGACATCT
    CAGGGCACCC AGACCCTGGC CCTTCAGCTG CAGAATGCCG AAACACAGAC GGAGAGGGAA
    ATCCAGGAGC CCGGGACAGC AGCTTCGGGT CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT
    GCGAGCGGGG AGGACGCCCT CAGCAGGATC CAGCGGCTGA TGGCGGAAGG GGGCATGACG
    GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGCAACAGC
    ATCATCGTCA GCCACCGCAT CCACCGCGGC TCCCAGACGG GCGCTGAGCC AACGGCCGCC
    CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT CGGGGACTGC TCCCGGAGCC TGGGCAGCTG
    GCGGAGCGGG GCCTGAGCCC CCGGACAGCC TCCTGGGACC AGCCTGGTGC CCCCGGGCGA
    GAGCCGCCCC AGCCCCCCCT GCCACCTCTG CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC
    CCCCTTCCTG GCACCGAAGG ACCAACGCTG CACTGCGACC TGACCAGCAA CAACCACCTT
    GCTGATGGCG GTGGCAGCGG CAGGGGGGAA GCTGCAGGCC CCAGCAGGGA GCCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20104
    Clone ID Related Accession (Same CDS sequence) XM_005631291.3 , XM_005631291.2
    Accession Version XM_005631291.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGATTATCTC CTGCTGCATA CTACGCCAGA
    GGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTATCAG
    CAGAACCGTC TCCGTTCTTC CACCTCCTCT TCCTCAGACA ACCAGGGCCC ATCAGTAGAG
    GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCGCCTTCG CTTGGACGCT TCGTTCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCGGGGATT TTTCACGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCCT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCAGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCGG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TAGCAGCTTT CATCCCTAGC
    AGCCAAAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCGTTGGC CCCCCATAAC
    CTGGGTGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTATCG
    CCAATGGGCC GATACGTAAT GGTGGGCTTG GCCTCGAGGA GGATCCTGCT GCATCCCTCC
    ACAGAGCACA TGGTGGCCCA AGTCTTCAGG CTGCAACAGG CCCATGGTGG CGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC
    ATCAACTCTG CCCGTTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTATGG TACCAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTAAATG AGACTGTCTT CACTGTCCAC TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA
    ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGTC TGATGAATGC AATTGGGCTG
    CAGCCCCGGA ACCCCACCAC CTCTGTGACA TCTCAGGGCA CCCAGACCCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACGGAGAGG GAAATCCAGG AGCCCGGGAC AGCAGCTTCG
    GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC GGTGCGAGCG GGGAGGACGC CCTCAGCAGG
    ATCCAGCGGC TGATGGCGGA AGGGGGCATG ACGGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AGCATCATCG TCAGCCACCG CATCCACCGC
    GGCTCCCAGA CGGGCGCTGA GCCAACGGCC GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC
    TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG CTGGCGGAGC GGGGCCTGAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG CGAGAGCCGC CCCAGCCCCC CCTGCCACCT
    CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT CTCCCCCTTC CTGGCACCGA AGGACCAACG
    CTGCACTGCG ACCTGACCAG CAACAACCAC CTTGCTGATG GCGGTGGCAG CGGCAGGGGG
    GAAGCTGCAG GCCCCAGCAG GGAGCCACGG AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631348.1
    CDS341..4159
    Translation

    Target ORF information:

    RefSeq Version XM_005631291.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631291.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGATTATCTC CTGCTGCATA CTACGCCAGA
    GGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTATCAG
    CAGAACCGTC TCCGTTCTTC CACCTCCTCT TCCTCAGACA ACCAGGGCCC ATCAGTAGAG
    GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCGCCTTCG CTTGGACGCT TCGTTCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCGGGGATT TTTCACGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCCT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCAGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCGG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TAGCAGCTTT CATCCCTAGC
    AGCCAAAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCGTTGGC CCCCCATAAC
    CTGGGTGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTATCG
    CCAATGGGCC GATACGTAAT GGTGGGCTTG GCCTCGAGGA GGATCCTGCT GCATCCCTCC
    ACAGAGCACA TGGTGGCCCA AGTCTTCAGG CTGCAACAGG CCCATGGTGG CGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC
    ATCAACTCTG CCCGTTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTATGG TACCAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTAAATG AGACTGTCTT CACTGTCCAC TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA
    ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGTC TGATGAATGC AATTGGGCTG
    CAGCCCCGGA ACCCCACCAC CTCTGTGACA TCTCAGGGCA CCCAGACCCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACGGAGAGG GAAATCCAGG AGCCCGGGAC AGCAGCTTCG
    GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC GGTGCGAGCG GGGAGGACGC CCTCAGCAGG
    ATCCAGCGGC TGATGGCGGA AGGGGGCATG ACGGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AGCATCATCG TCAGCCACCG CATCCACCGC
    GGCTCCCAGA CGGGCGCTGA GCCAACGGCC GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC
    TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG CTGGCGGAGC GGGGCCTGAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG CGAGAGCCGC CCCAGCCCCC CCTGCCACCT
    CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT CTCCCCCTTC CTGGCACCGA AGGACCAACG
    CTGCACTGCG ACCTGACCAG CAACAACCAC CTTGCTGATG GCGGTGGCAG CGGCAGGGGG
    GAAGCTGCAG GCCCCAGCAG GGAGCCACGG AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20104
    Clone ID Related Accession (Same CDS sequence) XM_005631291.3 , XM_005631291.2
    Accession Version XM_005631291.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGATTATCTC CTGCTGCATA CTACGCCAGA
    GGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTATCAG
    CAGAACCGTC TCCGTTCTTC CACCTCCTCT TCCTCAGACA ACCAGGGCCC ATCAGTAGAG
    GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCGCCTTCG CTTGGACGCT TCGTTCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCGGGGATT TTTCACGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCCT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCAGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCGG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TAGCAGCTTT CATCCCTAGC
    AGCCAAAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCGTTGGC CCCCCATAAC
    CTGGGTGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTATCG
    CCAATGGGCC GATACGTAAT GGTGGGCTTG GCCTCGAGGA GGATCCTGCT GCATCCCTCC
    ACAGAGCACA TGGTGGCCCA AGTCTTCAGG CTGCAACAGG CCCATGGTGG CGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC
    ATCAACTCTG CCCGTTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTATGG TACCAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTAAATG AGACTGTCTT CACTGTCCAC TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA
    ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGTC TGATGAATGC AATTGGGCTG
    CAGCCCCGGA ACCCCACCAC CTCTGTGACA TCTCAGGGCA CCCAGACCCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACGGAGAGG GAAATCCAGG AGCCCGGGAC AGCAGCTTCG
    GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC GGTGCGAGCG GGGAGGACGC CCTCAGCAGG
    ATCCAGCGGC TGATGGCGGA AGGGGGCATG ACGGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AGCATCATCG TCAGCCACCG CATCCACCGC
    GGCTCCCAGA CGGGCGCTGA GCCAACGGCC GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC
    TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG CTGGCGGAGC GGGGCCTGAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG CGAGAGCCGC CCCAGCCCCC CCTGCCACCT
    CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT CTCCCCCTTC CTGGCACCGA AGGACCAACG
    CTGCACTGCG ACCTGACCAG CAACAACCAC CTTGCTGATG GCGGTGGCAG CGGCAGGGGG
    GAAGCTGCAG GCCCCAGCAG GGAGCCACGG AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631348.1
    CDS770..4588
    Translation

    Target ORF information:

    RefSeq Version XM_005631291.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631291.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCATC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCC ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGC TGACAGGGTC TGAGTGGACC
    CGGACAGTGC TCAGTCTGAA CTCCCGCTCT GAGGCAGAAT CCATGCCTCC GCCCAGGACC
    AGTGCCTCTT CGGTGAGTTT GCTATCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAAGCG
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG
    TCAGATGGAG GAAGTGGCTC CAGCCAAAAC AACTCAGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTAGA CCATAGCCTG
    AGCGGAGAAG CCCCCCAGAC CCAACAGGCC CAGGAAATGC TCAATAACAA CATTGAGTCT
    GAGAGGCCAG GCCCCTCCCA CCAGCCCACC CCACACAGTA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAACCG CTGTCGCGCT TGCCACAATC TCCTGACCTT CAACAACGAC
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTA GCGAGGCCAG CTCCTCCTGG
    CAGGTCCCCA GCACCTTCGA GGGCATGCCG TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG
    CGGACTGAGG GCCAAACACC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CTAGCAGATC TGGAACTGTA TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG AGATTATCTC CTGCTGCATA CTACGCCAGA
    GGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTATCAG
    CAGAACCGTC TCCGTTCTTC CACCTCCTCT TCCTCAGACA ACCAGGGCCC ATCAGTAGAG
    GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCGCCTTCG CTTGGACGCT TCGTTCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCGGGGATT TTTCACGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCCT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCAGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCGG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TAGCAGCTTT CATCCCTAGC
    AGCCAAAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCGTTGGC CCCCCATAAC
    CTGGGTGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTATCG
    CCAATGGGCC GATACGTAAT GGTGGGCTTG GCCTCGAGGA GGATCCTGCT GCATCCCTCC
    ACAGAGCACA TGGTGGCCCA AGTCTTCAGG CTGCAACAGG CCCATGGTGG CGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC
    ATCAACTCTG CCCGTTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTATGG TACCAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTAAATG AGACTGTCTT CACTGTCCAC TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA
    ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGTC TGATGAATGC AATTGGGCTG
    CAGCCCCGGA ACCCCACCAC CTCTGTGACA TCTCAGGGCA CCCAGACCCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACGGAGAGG GAAATCCAGG AGCCCGGGAC AGCAGCTTCG
    GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC GGTGCGAGCG GGGAGGACGC CCTCAGCAGG
    ATCCAGCGGC TGATGGCGGA AGGGGGCATG ACGGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AGCATCATCG TCAGCCACCG CATCCACCGC
    GGCTCCCAGA CGGGCGCTGA GCCAACGGCC GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC
    TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG CTGGCGGAGC GGGGCCTGAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG CGAGAGCCGC CCCAGCCCCC CCTGCCACCT
    CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT CTCCCCCTTC CTGGCACCGA AGGACCAACG
    CTGCACTGCG ACCTGACCAG CAACAACCAC CTTGCTGATG GCGGTGGCAG CGGCAGGGGG
    GAAGCTGCAG GCCCCAGCAG GGAGCCACGG AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20105
    Clone ID Related Accession (Same CDS sequence) XM_540754.4 , XM_540754.5
    Accession Version XM_540754.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540754.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCAGAGGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTATCAGCAG
    AACCGTCTCC GTTCTTCCAC CTCCTCTTCC TCAGACAACC AGGGCCCATC AGTAGAGGGA
    ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AATGGTGACA GATCTAGGCA CCGAGCTCCA
    CGCAATGCCC GGATGTCTGC GCCTTCGCTT GGACGCTTCG TTCCAAGGCG TTTCTTGCTG
    CCTGAGTACT TGCCTTATGC GGGGATTTTT CACGAACGTG GACAGCCTGG CTTGGCTACT
    CACTCCTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCAGCTGTG
    GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC
    CTCCCGGAAA TCAGTAATGC TTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT
    GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC
    CAAAGGGGCT TTCCTGATGA AGGCATCCTG GCAGTGTACT CGTTGGCCCC CCATAACCTG
    GGTGAAATGC TCTACACCAA GCGATTTGGT CCCAATGCCA TTTCGGTGAG CCTATCGCCA
    ATGGGCCGAT ACGTAATGGT GGGCTTGGCC TCGAGGAGGA TCCTGCTGCA TCCCTCCACA
    GAGCACATGG TGGCCCAAGT CTTCAGGCTG CAACAGGCCC ATGGTGGCGA GACCTCCATG
    AGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC
    AACTCTGCCC GTTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTATGGTAC CAACAAAGGA
    GACCTGGTGA TCTGCCGACC AGAGGCCTTA AACTCTGGTG TTGAGTACTA CTGGGACCAG
    CTAAATGAGA CTGTCTTCAC TGTCCACTCC AGCAGCAGGA GCAGTGAAAG GCCTGGAACC
    AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGTCTGA TGAATGCAAT TGGGCTGCAG
    CCCCGGAACC CCACCACCTC TGTGACATCT CAGGGCACCC AGACCCTGGC CCTTCAGCTG
    CAGAATGCCG AAACACAGAC GGAGAGGGAA ATCCAGGAGC CCGGGACAGC AGCTTCGGGT
    CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT GCGAGCGGGG AGGACGCCCT CAGCAGGATC
    CAGCGGCTGA TGGCGGAAGG GGGCATGACG GCCGTGGTGC AGCGGGAGCA GAGCACCACC
    ATGGCCTCCA TGGGCGGCTT CGGCAACAGC ATCATCGTCA GCCACCGCAT CCACCGCGGC
    TCCCAGACGG GCGCTGAGCC AACGGCCGCC CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT
    CGGGGACTGC TCCCGGAGCC TGGGCAGCTG GCGGAGCGGG GCCTGAGCCC CCGGACAGCC
    TCCTGGGACC AGCCTGGTGC CCCCGGGCGA GAGCCGCCCC AGCCCCCCCT GCCACCTCTG
    CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC CCCCTTCCTG GCACCGAAGG ACCAACGCTG
    CACTGCGACC TGACCAGCAA CAACCACCTT GCTGATGGCG GTGGCAGCGG CAGGGGGGAA
    GCTGCAGGCC CCAGCAGGGA GCCACGGAAC AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540754.2
    CDS765..4400
    Translation

    Target ORF information:

    RefSeq Version XM_540754.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540754.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCAGAGGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTATCAGCAG
    AACCGTCTCC GTTCTTCCAC CTCCTCTTCC TCAGACAACC AGGGCCCATC AGTAGAGGGA
    ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AATGGTGACA GATCTAGGCA CCGAGCTCCA
    CGCAATGCCC GGATGTCTGC GCCTTCGCTT GGACGCTTCG TTCCAAGGCG TTTCTTGCTG
    CCTGAGTACT TGCCTTATGC GGGGATTTTT CACGAACGTG GACAGCCTGG CTTGGCTACT
    CACTCCTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCAGCTGTG
    GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC
    CTCCCGGAAA TCAGTAATGC TTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT
    GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC
    CAAAGGGGCT TTCCTGATGA AGGCATCCTG GCAGTGTACT CGTTGGCCCC CCATAACCTG
    GGTGAAATGC TCTACACCAA GCGATTTGGT CCCAATGCCA TTTCGGTGAG CCTATCGCCA
    ATGGGCCGAT ACGTAATGGT GGGCTTGGCC TCGAGGAGGA TCCTGCTGCA TCCCTCCACA
    GAGCACATGG TGGCCCAAGT CTTCAGGCTG CAACAGGCCC ATGGTGGCGA GACCTCCATG
    AGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC
    AACTCTGCCC GTTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTATGGTAC CAACAAAGGA
    GACCTGGTGA TCTGCCGACC AGAGGCCTTA AACTCTGGTG TTGAGTACTA CTGGGACCAG
    CTAAATGAGA CTGTCTTCAC TGTCCACTCC AGCAGCAGGA GCAGTGAAAG GCCTGGAACC
    AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGTCTGA TGAATGCAAT TGGGCTGCAG
    CCCCGGAACC CCACCACCTC TGTGACATCT CAGGGCACCC AGACCCTGGC CCTTCAGCTG
    CAGAATGCCG AAACACAGAC GGAGAGGGAA ATCCAGGAGC CCGGGACAGC AGCTTCGGGT
    CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT GCGAGCGGGG AGGACGCCCT CAGCAGGATC
    CAGCGGCTGA TGGCGGAAGG GGGCATGACG GCCGTGGTGC AGCGGGAGCA GAGCACCACC
    ATGGCCTCCA TGGGCGGCTT CGGCAACAGC ATCATCGTCA GCCACCGCAT CCACCGCGGC
    TCCCAGACGG GCGCTGAGCC AACGGCCGCC CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT
    CGGGGACTGC TCCCGGAGCC TGGGCAGCTG GCGGAGCGGG GCCTGAGCCC CCGGACAGCC
    TCCTGGGACC AGCCTGGTGC CCCCGGGCGA GAGCCGCCCC AGCCCCCCCT GCCACCTCTG
    CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC CCCCTTCCTG GCACCGAAGG ACCAACGCTG
    CACTGCGACC TGACCAGCAA CAACCACCTT GCTGATGGCG GTGGCAGCGG CAGGGGGGAA
    GCTGCAGGCC CCAGCAGGGA GCCACGGAAC AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20105
    Clone ID Related Accession (Same CDS sequence) XM_540754.4 , XM_540754.5
    Accession Version XM_540754.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540754.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCAGAGGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTATCAGCAG
    AACCGTCTCC GTTCTTCCAC CTCCTCTTCC TCAGACAACC AGGGCCCATC AGTAGAGGGA
    ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AATGGTGACA GATCTAGGCA CCGAGCTCCA
    CGCAATGCCC GGATGTCTGC GCCTTCGCTT GGACGCTTCG TTCCAAGGCG TTTCTTGCTG
    CCTGAGTACT TGCCTTATGC GGGGATTTTT CACGAACGTG GACAGCCTGG CTTGGCTACT
    CACTCCTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCAGCTGTG
    GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC
    CTCCCGGAAA TCAGTAATGC TTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT
    GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC
    CAAAGGGGCT TTCCTGATGA AGGCATCCTG GCAGTGTACT CGTTGGCCCC CCATAACCTG
    GGTGAAATGC TCTACACCAA GCGATTTGGT CCCAATGCCA TTTCGGTGAG CCTATCGCCA
    ATGGGCCGAT ACGTAATGGT GGGCTTGGCC TCGAGGAGGA TCCTGCTGCA TCCCTCCACA
    GAGCACATGG TGGCCCAAGT CTTCAGGCTG CAACAGGCCC ATGGTGGCGA GACCTCCATG
    AGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC
    AACTCTGCCC GTTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTATGGTAC CAACAAAGGA
    GACCTGGTGA TCTGCCGACC AGAGGCCTTA AACTCTGGTG TTGAGTACTA CTGGGACCAG
    CTAAATGAGA CTGTCTTCAC TGTCCACTCC AGCAGCAGGA GCAGTGAAAG GCCTGGAACC
    AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGTCTGA TGAATGCAAT TGGGCTGCAG
    CCCCGGAACC CCACCACCTC TGTGACATCT CAGGGCACCC AGACCCTGGC CCTTCAGCTG
    CAGAATGCCG AAACACAGAC GGAGAGGGAA ATCCAGGAGC CCGGGACAGC AGCTTCGGGT
    CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT GCGAGCGGGG AGGACGCCCT CAGCAGGATC
    CAGCGGCTGA TGGCGGAAGG GGGCATGACG GCCGTGGTGC AGCGGGAGCA GAGCACCACC
    ATGGCCTCCA TGGGCGGCTT CGGCAACAGC ATCATCGTCA GCCACCGCAT CCACCGCGGC
    TCCCAGACGG GCGCTGAGCC AACGGCCGCC CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT
    CGGGGACTGC TCCCGGAGCC TGGGCAGCTG GCGGAGCGGG GCCTGAGCCC CCGGACAGCC
    TCCTGGGACC AGCCTGGTGC CCCCGGGCGA GAGCCGCCCC AGCCCCCCCT GCCACCTCTG
    CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC CCCCTTCCTG GCACCGAAGG ACCAACGCTG
    CACTGCGACC TGACCAGCAA CAACCACCTT GCTGATGGCG GTGGCAGCGG CAGGGGGGAA
    GCTGCAGGCC CCAGCAGGGA GCCACGGAAC AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540754.2
    CDS340..3975
    Translation

    Target ORF information:

    RefSeq Version XM_540754.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540754.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCAGAGGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTATCAGCAG
    AACCGTCTCC GTTCTTCCAC CTCCTCTTCC TCAGACAACC AGGGCCCATC AGTAGAGGGA
    ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AATGGTGACA GATCTAGGCA CCGAGCTCCA
    CGCAATGCCC GGATGTCTGC GCCTTCGCTT GGACGCTTCG TTCCAAGGCG TTTCTTGCTG
    CCTGAGTACT TGCCTTATGC GGGGATTTTT CACGAACGTG GACAGCCTGG CTTGGCTACT
    CACTCCTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCAGCTGTG
    GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC
    CTCCCGGAAA TCAGTAATGC TTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT
    GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTAG CAGCTTTCAT CCCTAGCAGC
    CAAAGGGGCT TTCCTGATGA AGGCATCCTG GCAGTGTACT CGTTGGCCCC CCATAACCTG
    GGTGAAATGC TCTACACCAA GCGATTTGGT CCCAATGCCA TTTCGGTGAG CCTATCGCCA
    ATGGGCCGAT ACGTAATGGT GGGCTTGGCC TCGAGGAGGA TCCTGCTGCA TCCCTCCACA
    GAGCACATGG TGGCCCAAGT CTTCAGGCTG CAACAGGCCC ATGGTGGCGA GACCTCCATG
    AGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC
    AACTCTGCCC GTTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTATGGTAC CAACAAAGGA
    GACCTGGTGA TCTGCCGACC AGAGGCCTTA AACTCTGGTG TTGAGTACTA CTGGGACCAG
    CTAAATGAGA CTGTCTTCAC TGTCCACTCC AGCAGCAGGA GCAGTGAAAG GCCTGGAACC
    AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGTCTGA TGAATGCAAT TGGGCTGCAG
    CCCCGGAACC CCACCACCTC TGTGACATCT CAGGGCACCC AGACCCTGGC CCTTCAGCTG
    CAGAATGCCG AAACACAGAC GGAGAGGGAA ATCCAGGAGC CCGGGACAGC AGCTTCGGGT
    CCTGGTGAAG GCGAAGGCTC AGAGTCCGGT GCGAGCGGGG AGGACGCCCT CAGCAGGATC
    CAGCGGCTGA TGGCGGAAGG GGGCATGACG GCCGTGGTGC AGCGGGAGCA GAGCACCACC
    ATGGCCTCCA TGGGCGGCTT CGGCAACAGC ATCATCGTCA GCCACCGCAT CCACCGCGGC
    TCCCAGACGG GCGCTGAGCC AACGGCCGCC CGCGCCTCCT CGCCCCGGCC CTCCGCCTCT
    CGGGGACTGC TCCCGGAGCC TGGGCAGCTG GCGGAGCGGG GCCTGAGCCC CCGGACAGCC
    TCCTGGGACC AGCCTGGTGC CCCCGGGCGA GAGCCGCCCC AGCCCCCCCT GCCACCTCTG
    CCCTCTTCCT CCCCTGTCCC TGCCCCTCTC CCCCTTCCTG GCACCGAAGG ACCAACGCTG
    CACTGCGACC TGACCAGCAA CAACCACCTT GCTGATGGCG GTGGCAGCGG CAGGGGGGAA
    GCTGCAGGCC CCAGCAGGGA GCCACGGAAC AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20106
    Clone ID Related Accession (Same CDS sequence) XM_005631292.3 , XM_005631292.2
    Accession Version XM_005631292.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCAGA GGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTATCAG CAGAACCGTC TCCGTTCTTC CACCTCCTCT
    TCCTCAGACA ACCAGGGCCC ATCAGTAGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG
    GACAATGGTG ACAGATCTAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCGCCTTCG
    CTTGGACGCT TCGTTCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCGGGGATT
    TTTCACGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCCT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TTGGTGATGG ACAGTCAGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TAGCAGCTTT CATCCCTAGC AGCCAAAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCGTTGGC CCCCCATAAC CTGGGTGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTATCG CCAATGGGCC GATACGTAAT GGTGGGCTTG
    GCCTCGAGGA GGATCCTGCT GCATCCCTCC ACAGAGCACA TGGTGGCCCA AGTCTTCAGG
    CTGCAACAGG CCCATGGTGG CGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTACCCC
    ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAACTCTG CCCGTTGGCT GCCCGAGCCA
    GGGCTTGGCC TGGCCTATGG TACCAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTAAATG AGACTGTCTT CACTGTCCAC
    TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA
    GACATGGGTC TGATGAATGC AATTGGGCTG CAGCCCCGGA ACCCCACCAC CTCTGTGACA
    TCTCAGGGCA CCCAGACCCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACGGAGAGG
    GAAATCCAGG AGCCCGGGAC AGCAGCTTCG GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC
    GGTGCGAGCG GGGAGGACGC CCTCAGCAGG ATCCAGCGGC TGATGGCGGA AGGGGGCATG
    ACGGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AGCATCATCG TCAGCCACCG CATCCACCGC GGCTCCCAGA CGGGCGCTGA GCCAACGGCC
    GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG
    CTGGCGGAGC GGGGCCTGAG CCCCCGGACA GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG
    CGAGAGCCGC CCCAGCCCCC CCTGCCACCT CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT
    CTCCCCCTTC CTGGCACCGA AGGACCAACG CTGCACTGCG ACCTGACCAG CAACAACCAC
    CTTGCTGATG GCGGTGGCAG CGGCAGGGGG GAAGCTGCAG GCCCCAGCAG GGAGCCACGG
    AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631349.1
    CDS340..3888
    Translation

    Target ORF information:

    RefSeq Version XM_005631292.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631292.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCAGA GGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTATCAG CAGAACCGTC TCCGTTCTTC CACCTCCTCT
    TCCTCAGACA ACCAGGGCCC ATCAGTAGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG
    GACAATGGTG ACAGATCTAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCGCCTTCG
    CTTGGACGCT TCGTTCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCGGGGATT
    TTTCACGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCCT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TTGGTGATGG ACAGTCAGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TAGCAGCTTT CATCCCTAGC AGCCAAAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCGTTGGC CCCCCATAAC CTGGGTGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTATCG CCAATGGGCC GATACGTAAT GGTGGGCTTG
    GCCTCGAGGA GGATCCTGCT GCATCCCTCC ACAGAGCACA TGGTGGCCCA AGTCTTCAGG
    CTGCAACAGG CCCATGGTGG CGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTACCCC
    ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAACTCTG CCCGTTGGCT GCCCGAGCCA
    GGGCTTGGCC TGGCCTATGG TACCAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTAAATG AGACTGTCTT CACTGTCCAC
    TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA
    GACATGGGTC TGATGAATGC AATTGGGCTG CAGCCCCGGA ACCCCACCAC CTCTGTGACA
    TCTCAGGGCA CCCAGACCCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACGGAGAGG
    GAAATCCAGG AGCCCGGGAC AGCAGCTTCG GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC
    GGTGCGAGCG GGGAGGACGC CCTCAGCAGG ATCCAGCGGC TGATGGCGGA AGGGGGCATG
    ACGGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AGCATCATCG TCAGCCACCG CATCCACCGC GGCTCCCAGA CGGGCGCTGA GCCAACGGCC
    GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG
    CTGGCGGAGC GGGGCCTGAG CCCCCGGACA GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG
    CGAGAGCCGC CCCAGCCCCC CCTGCCACCT CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT
    CTCCCCCTTC CTGGCACCGA AGGACCAACG CTGCACTGCG ACCTGACCAG CAACAACCAC
    CTTGCTGATG GCGGTGGCAG CGGCAGGGGG GAAGCTGCAG GCCCCAGCAG GGAGCCACGG
    AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20106
    Clone ID Related Accession (Same CDS sequence) XM_005631292.3 , XM_005631292.2
    Accession Version XM_005631292.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCAGA GGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTATCAG CAGAACCGTC TCCGTTCTTC CACCTCCTCT
    TCCTCAGACA ACCAGGGCCC ATCAGTAGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG
    GACAATGGTG ACAGATCTAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCGCCTTCG
    CTTGGACGCT TCGTTCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCGGGGATT
    TTTCACGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCCT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TTGGTGATGG ACAGTCAGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TAGCAGCTTT CATCCCTAGC AGCCAAAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCGTTGGC CCCCCATAAC CTGGGTGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTATCG CCAATGGGCC GATACGTAAT GGTGGGCTTG
    GCCTCGAGGA GGATCCTGCT GCATCCCTCC ACAGAGCACA TGGTGGCCCA AGTCTTCAGG
    CTGCAACAGG CCCATGGTGG CGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTACCCC
    ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAACTCTG CCCGTTGGCT GCCCGAGCCA
    GGGCTTGGCC TGGCCTATGG TACCAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTAAATG AGACTGTCTT CACTGTCCAC
    TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA
    GACATGGGTC TGATGAATGC AATTGGGCTG CAGCCCCGGA ACCCCACCAC CTCTGTGACA
    TCTCAGGGCA CCCAGACCCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACGGAGAGG
    GAAATCCAGG AGCCCGGGAC AGCAGCTTCG GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC
    GGTGCGAGCG GGGAGGACGC CCTCAGCAGG ATCCAGCGGC TGATGGCGGA AGGGGGCATG
    ACGGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AGCATCATCG TCAGCCACCG CATCCACCGC GGCTCCCAGA CGGGCGCTGA GCCAACGGCC
    GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG
    CTGGCGGAGC GGGGCCTGAG CCCCCGGACA GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG
    CGAGAGCCGC CCCAGCCCCC CCTGCCACCT CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT
    CTCCCCCTTC CTGGCACCGA AGGACCAACG CTGCACTGCG ACCTGACCAG CAACAACCAC
    CTTGCTGATG GCGGTGGCAG CGGCAGGGGG GAAGCTGCAG GCCCCAGCAG GGAGCCACGG
    AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631349.1
    CDS764..4312
    Translation

    Target ORF information:

    RefSeq Version XM_005631292.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631292.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATACTAT GGGGGCGAGA ACGGGGCACT 
    CGGGCCTTTG GAGCTCAGCG GCTTCTTCAG GAGCTAGTGG AAGATAAAAC TCGGTGGATG
    AAATGGGAAG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCTACCTT TTTACTGGCC
    TTCAGCCCAG ACAGGACTCT ATTGGCCTCC ACCCACGTGA ACCATAATAT CTATATTACG
    GAAGTGAAGA CTGGTAAATG TGTTCATTCT CTGATTGGAC ACCGCCGTAC TCCGTGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTC
    AGGATTTGGG ATTTGCATGG TGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATT
    GCTTCTCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ATTGGAGTCG GCGGGAGCCC TTTGCTGTGG TGAAGACAGC TAGCGAGATG
    GAGCGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCCATTGTT
    AACCCTTCCA ATCAGCAGGG TGATGATGAA CCAGAGATCC CCATCGACGG AACAGAGTTA
    TCCCACTACC GTCAGCGTGC TCTGCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCC
    ACCCGTCGCT CTTTGGGAGG CCCTCTGTCC AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGC TGACAGGGTC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCCCGCTCT
    GAGGCAGAAT CCATGCCTCC GCCCAGGACC AGTGCCTCTT CGGTGAGTTT GCTATCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAAGCG TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAA CTTCAGGGTT GGCAGCTGAG TCAGATGGAG GAAGTGGCTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTAGA CCATAGCCTG AGCGGAGAAG CCCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAATAACAA CATTGAGTCT GAGAGGCCAG GCCCCTCCCA CCAGCCCACC
    CCACACAGTA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGTCGCGCT
    TGCCACAATC TCCTGACCTT CAACAACGAC ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTA GCGAGGCCAG CTCCTCCTGG CAGGTCCCCA GCACCTTCGA GGGCATGCCG
    TCAAGTGGCA GCCAGTTGCC ACCCCTTGAG CGGACTGAGG GCCAAACACC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CTAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAAGA TTCGCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCAGA GGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTATCAG CAGAACCGTC TCCGTTCTTC CACCTCCTCT
    TCCTCAGACA ACCAGGGCCC ATCAGTAGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG
    GACAATGGTG ACAGATCTAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCGCCTTCG
    CTTGGACGCT TCGTTCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCGGGGATT
    TTTCACGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCCT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TTGGTGATGG ACAGTCAGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TAGCAGCTTT CATCCCTAGC AGCCAAAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCGTTGGC CCCCCATAAC CTGGGTGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTATCG CCAATGGGCC GATACGTAAT GGTGGGCTTG
    GCCTCGAGGA GGATCCTGCT GCATCCCTCC ACAGAGCACA TGGTGGCCCA AGTCTTCAGG
    CTGCAACAGG CCCATGGTGG CGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTACCCC
    ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAACTCTG CCCGTTGGCT GCCCGAGCCA
    GGGCTTGGCC TGGCCTATGG TACCAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTAAATG AGACTGTCTT CACTGTCCAC
    TCCAGCAGCA GGAGCAGTGA AAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA
    GACATGGGTC TGATGAATGC AATTGGGCTG CAGCCCCGGA ACCCCACCAC CTCTGTGACA
    TCTCAGGGCA CCCAGACCCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACGGAGAGG
    GAAATCCAGG AGCCCGGGAC AGCAGCTTCG GGTCCTGGTG AAGGCGAAGG CTCAGAGTCC
    GGTGCGAGCG GGGAGGACGC CCTCAGCAGG ATCCAGCGGC TGATGGCGGA AGGGGGCATG
    ACGGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AGCATCATCG TCAGCCACCG CATCCACCGC GGCTCCCAGA CGGGCGCTGA GCCAACGGCC
    GCCCGCGCCT CCTCGCCCCG GCCCTCCGCC TCTCGGGGAC TGCTCCCGGA GCCTGGGCAG
    CTGGCGGAGC GGGGCCTGAG CCCCCGGACA GCCTCCTGGG ACCAGCCTGG TGCCCCCGGG
    CGAGAGCCGC CCCAGCCCCC CCTGCCACCT CTGCCCTCTT CCTCCCCTGT CCCTGCCCCT
    CTCCCCCTTC CTGGCACCGA AGGACCAACG CTGCACTGCG ACCTGACCAG CAACAACCAC
    CTTGCTGATG GCGGTGGCAG CGGCAGGGGG GAAGCTGCAG GCCCCAGCAG GGAGCCACGG
    AACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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