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BEST1 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol BEST1
Entrez Gene ID 483791
Full Name bestrophin 1
Synonyms VMD2
General protein information
Preferred Names

bestrophin 1

Names

bestrophin-1
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_022405085.1 XP_022260793.1 bestrophin-1 isoform X2
XM_014120876.2 XP_013976351.1 bestrophin-1 isoform X1
XM_022405083.1 XP_022260791.1 bestrophin-1 isoform X1
XM_014120876.1 XP_013976351.1 bestrophin-1 isoform X1
NM_001097545.1 NP_001091014.1 bestrophin-1
XM_022405084.1 XP_022260792.1 bestrophin-1 isoform X1
XM_022405086.1 XP_022260794.1 bestrophin-1 isoform X3

Pathways from BioSystems


Homologies


Homo sapiens (human) BEST1 NP_004174.1
Bos taurus (cattle) BEST1 NP_001073714.1
Mus musculus (house mouse) Best1 NP_036043.2
Danio rerio (zebrafish) LOC560608 XP_689098.3
Pan troglodytes (chimpanzee) BEST1 XP_001151529.1
LOC718993 XP_001116591.1
Xenopus tropicalis (tropical clawed frog) best1 NP_001155223.1
Canis lupus familiaris (dog) BEST1 NP_001091014.1
Rattus norvegicus (Norway rat) Best1 NP_001011940.1
Gallus gallus (chicken) BEST1 XP_004941545.1

Gene Ontology


Bibliography

Related articles in PubMed

Modeling the structural consequences of BEST1 missense mutations.
Guziewicz KE, Aguirre GD, Zangerl B
Advances in experimental medicine and biology723611-8(2012)

Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).
Zangerl B, Wickstr?m K, Slavik J, Lindauer SJ, Ahonen S, Schelling C, Lohi H, Guziewicz KE, Aguirre GD
Molecular vision162791-804(2010 Dec)

Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.
Aguirre-Hern?ndez J, Sargan DR
Animal genetics41(3)333-4(2010 Jun)

Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.
Guziewicz KE, Zangerl B, Lindauer SJ, Mullins RF, Sandmeyer LS, Grahn BH, Stone EM, Acland GM, Aguirre GD
Investigative ophthalmology & visual science48(5)1959-67(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bestrophinopathies are a group of inherited retinal disorders primarily caused by point mutations scattered throughout the BEST1 gene.
Title: Modeling the structural consequences of BEST1 missense mutations.

Seven novel coding variants were identified in exon 10 of cBEST1.
Title: Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).

The mutation underlying Multifocal retinal dysplasia in the German Spitz is not allelic with BEST1 mutations found in four other breeds.
Title: Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.

Two spontaneous mutations in the canine VMD2 gene, which cause canine multifocal retinopathy, as the first naturally occurring animal model of BMD (Best macular dystrophy).
Title: Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.

The following BEST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe33954 NM_001097545.1
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OCe33954 XM_022405085.1
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OCe294596 XM_022405086.1
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe41041 XM_022405084.1
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe41041 XM_022405083.1
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe41041 XM_014120876.2
Latest version!		 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe41041 XM_014120876.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe33954
Clone ID Related Accession (Same CDS sequence) XM_022405085.1 , NM_001097545.1
Accession Version NM_001097545.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-22
Organism Canis lupus familiaris(dog)
Product bestrophin-1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF110978.1. On May 24, 2007 this sequence version replaced XM_540912.2. ##Evidence-Data-START## Transcript exon combination :: EF110978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
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361
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481
541
601
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721
781
841
901
961
1021
1081
1141
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1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001091014.1
CDS110..1852
Translation

Target ORF information:

RefSeq Version NM_001097545.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001097545.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe33954
Clone ID Related Accession (Same CDS sequence) XM_022405085.1 , NM_001097545.1
Accession Version XM_022405085.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product bestrophin-1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022260793.1
CDS597..2339
Translation

Target ORF information:

RefSeq Version XM_022405085.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022405085.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe294596
Clone ID Related Accession (Same CDS sequence) XM_022405086.1
Accession Version XM_022405086.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1602bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product bestrophin-1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCCCTCA CGGAGGAACA GCAGGTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACTCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAA CCTGGTGTCG 
GGCTTGGTGG AGGGCAGGGA CGAGCAGGGC CGGCTGCTGC GCCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCG CCGCCGTCTA CAAGCGCTTC 
CCCAGCTCCC AGCACCTGGT GAAAGCAGGC TTTATGACCC CCGCGGAATG CAAGCACTTA 
GAAAAGCTGA GCTTGCCGCA CAACACGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGATTGG AGGTCGAATC CGGGACCCTG TCCTGCTCCA GAGCCTGCTG 
GACGAGATGA ACATCTTGCG TACTCAGTGT GGACACCTGT TTGCCTACGA CTGGATCAGT 
ATCCCGCTGG TGTACACGCA GGTGGTGACC GTGGCTGTAT ACAGCTTCTT CCTGGCTTGC 
CTGGTTGGGC GGCAGTTCCT GAACCCAGCC AAGGCCTACC CTGGCCACGA GCTGGACCTT 
GTTGTACCTG TCTTCACATT TCTGCAGTTC TTCTTCTATG CTGGCTGGCT GAAGTGGTGC 
TGGGAGGTGG CAGAACAGCT CATCAACCCA TTTGGAGAGG ATGATGATGA CTTTGAGACC 
AACTGGATTG TTGACAGGAG TTTGCAGGTG TCCCTGTTGG CTGTGGACGA GATGCACCAG 
GACCTGCCCC CGATGGAGCG GGATATGTAC TGGAATGAAC CAGAACCACA ACCCCCCTAC 
ACGGCTGCTT CTGCCCAGTA TCGTCGAGCC TCCTTTTTAG GCTCCACTTT CAACATCAAT 
CTGCATAAGG AAGACATGGA GGAGGAAGAG GAAGGCACTC ACGCTGGCAT CATTGGCCGC 
TTTCTAGGGC TGCAGTCCCA CGACCACCAA CCCCCCAGGA CAAACTCAAA GACCAAACTA 
CTATGGCCCA AGAAAGAAGG CTTTCTCCAA GACGGCCAGC CCAAGAAACT CGGGGGTGCC 
AGGCAGACCT CTAGGGACAA GGAAGACAGC AAGGCCTGGG AGATTAAGGA GGAGGGTGCT 
TTTGAGACTG CTGCATTTTA TGGGAGGTCA GGCTACCACA GTGCCCCACA GACGCCCCTC 
AGCCACACAC CTATGGTCTT CCCATCTGGA CAGTCAGCAC CCTCAAGCCT ACGCAGAGTC 
TCAGGGATAG ACGGCGCTGT CAAAGACCAA AGCCTACAGC CTGGGATAAA GACCAGTTCT 
GAAGCACTCC CAGAGAATGT TGGGTTCTCA TTAGAGAACC CAGAATCGGG TCACGTGAAG 
AGGAAAACTG TCGAGTTTAA CCTGACAGAC ATGTCGGGGA CCCCTGAACA TCATCTCAGA 
GACTCAGATT TGAGCCAGTC AACTAGCAAC TTACACACTA TACTCAAGGG CCACGGAGAT 
CCCTATTGGG CCTTGGAAAA CAGGGATGAA GCACATTCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022260794.1
CDS136..1737
Translation

Target ORF information:

RefSeq Version XM_022405086.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022405086.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCCCTCA CGGAGGAACA GCAGGTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACTCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAA CCTGGTGTCG 
GGCTTGGTGG AGGGCAGGGA CGAGCAGGGC CGGCTGCTGC GCCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCG CCGCCGTCTA CAAGCGCTTC 
CCCAGCTCCC AGCACCTGGT GAAAGCAGGC TTTATGACCC CCGCGGAATG CAAGCACTTA 
GAAAAGCTGA GCTTGCCGCA CAACACGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGATTGG AGGTCGAATC CGGGACCCTG TCCTGCTCCA GAGCCTGCTG 
GACGAGATGA ACATCTTGCG TACTCAGTGT GGACACCTGT TTGCCTACGA CTGGATCAGT 
ATCCCGCTGG TGTACACGCA GGTGGTGACC GTGGCTGTAT ACAGCTTCTT CCTGGCTTGC 
CTGGTTGGGC GGCAGTTCCT GAACCCAGCC AAGGCCTACC CTGGCCACGA GCTGGACCTT 
GTTGTACCTG TCTTCACATT TCTGCAGTTC TTCTTCTATG CTGGCTGGCT GAAGTGGTGC 
TGGGAGGTGG CAGAACAGCT CATCAACCCA TTTGGAGAGG ATGATGATGA CTTTGAGACC 
AACTGGATTG TTGACAGGAG TTTGCAGGTG TCCCTGTTGG CTGTGGACGA GATGCACCAG 
GACCTGCCCC CGATGGAGCG GGATATGTAC TGGAATGAAC CAGAACCACA ACCCCCCTAC 
ACGGCTGCTT CTGCCCAGTA TCGTCGAGCC TCCTTTTTAG GCTCCACTTT CAACATCAAT 
CTGCATAAGG AAGACATGGA GGAGGAAGAG GAAGGCACTC ACGCTGGCAT CATTGGCCGC 
TTTCTAGGGC TGCAGTCCCA CGACCACCAA CCCCCCAGGA CAAACTCAAA GACCAAACTA 
CTATGGCCCA AGAAAGAAGG CTTTCTCCAA GACGGCCAGC CCAAGAAACT CGGGGGTGCC 
AGGCAGACCT CTAGGGACAA GGAAGACAGC AAGGCCTGGG AGATTAAGGA GGAGGGTGCT 
TTTGAGACTG CTGCATTTTA TGGGAGGTCA GGCTACCACA GTGCCCCACA GACGCCCCTC 
AGCCACACAC CTATGGTCTT CCCATCTGGA CAGTCAGCAC CCTCAAGCCT ACGCAGAGTC 
TCAGGGATAG ACGGCGCTGT CAAAGACCAA AGCCTACAGC CTGGGATAAA GACCAGTTCT 
GAAGCACTCC CAGAGAATGT TGGGTTCTCA TTAGAGAACC CAGAATCGGG TCACGTGAAG 
AGGAAAACTG TCGAGTTTAA CCTGACAGAC ATGTCGGGGA CCCCTGAACA TCATCTCAGA 
GACTCAGATT TGAGCCAGTC AACTAGCAAC TTACACACTA TACTCAAGGG CCACGGAGAT 
CCCTATTGGG CCTTGGAAAA CAGGGATGAA GCACATTCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe41041
Clone ID Related Accession (Same CDS sequence) XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version XM_014120876.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product bestrophin-1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013976351.1
CDS661..2415
Translation

Target ORF information:

RefSeq Version XM_014120876.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014120876.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe41041
Clone ID Related Accession (Same CDS sequence) XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version XM_022405083.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product bestrophin-1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022260791.1
CDS598..2352
Translation

Target ORF information:

RefSeq Version XM_022405083.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022405083.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe41041
Clone ID Related Accession (Same CDS sequence) XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version XM_014120876.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product bestrophin-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013976351.1
CDS701..2455
Translation

Target ORF information:

RefSeq Version XM_014120876.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014120876.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe41041
Clone ID Related Accession (Same CDS sequence) XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version XM_022405084.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product bestrophin-1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022260792.1
CDS173..1927
Translation

Target ORF information:

RefSeq Version XM_022405084.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022405084.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Modeling the structural consequences of BEST1 missense mutations.
Advances in experimental medicine and biology723611-8(2012)
Guziewicz KE,Aguirre GD,Zangerl B


book

Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).
Molecular vision162791-804(2010 Dec)
Zangerl B,Wickstr?m K,Slavik J,Lindauer SJ,Ahonen S,Schelling C,Lohi H,Guziewicz KE,Aguirre GD


book

Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.
Animal genetics41(3)333-4(2010 Jun)
Aguirre-Hern?ndez J,Sargan DR


book

Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.
Investigative ophthalmology & visual science48(5)1959-67(2007 May)
Guziewicz KE,Zangerl B,Lindauer SJ,Mullins RF,Sandmeyer LS,Grahn BH,Stone EM,Acland GM,Aguirre GD