MYO7B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO7B
Entrez Gene ID	483867
Full Name	myosin VIIB
General protein information	Preferred Names myosin VIIB Names myosin-VIIb unconventional myosin-VIIb
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014121386.1	XP_013976861.1	unconventional myosin-VIIb isoform X3
XM_014121387.2	XP_013976862.1	unconventional myosin-VIIb isoform X1
XM_022406008.1	XP_022261716.1	unconventional myosin-VIIb isoform X2
XM_022406009.1	XP_022261717.1	unconventional myosin-VIIb isoform X3
XM_014121384.1	XP_013976859.1	unconventional myosin-VIIb isoform X1
XM_014121385.1	XP_013976860.1	unconventional myosin-VIIb isoform X2
XM_014121387.1	XP_013976862.1	unconventional myosin-VIIb isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myo7b	NP_001178870.1
Gallus gallus (chicken)	MYO7B	XP_003641791.1
	MYO7B	XP_002799468.1
Bos taurus (cattle)	MYO7B	XP_002685259.2
Xenopus tropicalis (tropical clawed frog)	myo7b	XP_002936659.2
Mus musculus (house mouse)	Myo7b	NP_115770.2
Homo sapiens (human)	MYO7B	NP_001073996.1
Pan troglodytes (chimpanzee)	MYO7B	XP_515778.3
Canis lupus familiaris (dog)	MYO7B	XP_005631987.1
Caenorhabditis elegans (roundworm)	hum-6	NP_508420.1

Gene Ontology

The following MYO7B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO7B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe295084	XM_022406009.1 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe295083	XM_022406008.1 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe41358	XM_014121384.1 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe41359	XM_014121385.1 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe41360	XM_014121386.1 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe41361	XM_014121387.2 Latest version!	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
View Old Accession Versions >>
OCe41361	XM_014121387.1	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO7B ( XM_022406009.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022261717.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.

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CloneID	OCe295084
Clone ID Related Accession (Same CDS sequence)	XM_022406009.1
Accession Version	XM_022406009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	unconventional myosin-VIIb isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCA GGCTCTCTGG AACACCTGCC CCTCCAGGCT CCCGAGGAGC GGAACTACCA 
CATCTTCTAC GGCATGCTCC TGGGGATGAC CGCGGAGGAG AAGAAGCTGC TGAGCCTGGG 
GACGCCCTCC GAGTACCACT ACCTGACCAC GCTGCAGTCT TCGAGAACCT GGATTCCTCC 
GACGTGATGG AGACGCCTGC CTTTCCCACC GTGCTGAAGT TACTGGAGGT GGAGTACCAG 
GCACTCCGGG ACTGTCTGAT CAAGCATTCC ATCATCATCC GAGGGGAGTT TGTCACCAGG 
CCCCTGAACA TCACTCAGGC TGCAGACCGG AGGGATGCCT TTGTCAAGGG CATCTATGGG 
CACCTCTTCC TGTGGATTGT CAAGAAGATC AACACTGCAA TTTTCACTCC ACCTGCCCAG 
GACCCCAAAA ATGTGCGAAG AGCCATTGGC CTCCTGGACA TATTTGGCTT TGAAAATTTC 
CAGAACAATA GCTTCGAGCA GCTCTGCATC AACTTTGCCA ACGAGCACCT GCAGCAGTTC 
TTCGTGCAGC ATGTGTTCAC CATGGAGCAG GAGGAGTACC TCTCAGAGAA CATTGCCTGG 
GACTACATCC ACTACAATGA CAATCGGCCC ACCCTGGATC TGCTGGCCCT TAAGCCCATG 
AGCATCATCT CCCTCCTGGA CGAGGAGAGC CACTTCCCAC AGGGGACAGA TACAACCATG 
CTGCAGAAAC TGAACAGTGT CCATGCCAAC AATAAGGCCT ACCTACAGCC CAGGAACATC 
CATGATGCCA GGTTTGGCAT CGCCCACTTT GCAGGCGAGG TGTACTACCA GACAGAAGGC 
TTCCTGGAGA AGAATCGAGA CATGCTGAGC ACAGATATCC TCACTCTGGT CTACTCCTCC 
AAAAACAAGT TCCTGAAGGA GATATTCAAG CTGGAGTCAG CGGGGACCAA GCTGGGCCAA 
AGCACCATCA TCCAGGCTAG CGCAGGGAGC CAGCTCTTCA AGTCTGCAGA CTCCAGCAAG 
CAGCCCTCCA CATTGGCAGG CCAGTTCAAG AAGTCCCTGG ACCAGCTGAT GAAAATCCTG 
ACCAGCTGCC AGCCCTACTT TATCCGCTGC ATCAAACCCA ATGAGTACAA GAAGCCACTG 
CTCTTCGACC GCGACCTGTG CCTCCGGCAG CTGCGTTACT CGGGGATGAT GGAGACCGTG 
CACATCCGCA GGTCCGGCTT CCCCATCCGG TACACGTTCC AGGAGTTCGC GCAGAGGTTC 
GGCGTGCTGC TGCCCAGCGC CGTTCGCCTG CAGCTTCGAG ACAAGGCTCG CCAGATGACC 
CTGCGCATTG CTGAAATGCA GCTGGGGACG GACAAAGAAT GGAAAGTGGG AAAGACCAAA 
ATTTTCCTGA AGGATAACCA GGACACTCTA CTTGAGGTAC AGAGGACCCA GACCCTGGAC 
AAGGCAGCAA TCAACATCCA AAGAGTACTG CGAGGCTACA AATACAGGAA GGAGTTCTTG 
AGACAGAGGC GGGCCGCTGT GACCCTCCAG GCCAGGTGGA GAGGCTACTA CAACAAGCGG 
AACTTCAAGC AGATCCTCTT GGGTTTTGAG CGCCTGCAGG CCATTGCCCG GAGCCAGTGG 
CTAGCAAAGC AGTACCAGAC CATGCGGCAG AGGATGGTCC AGCTGCAGGC ACTCTGCAGG 
GGCTACCTTG TGCGCCAGCA AGTCCAGGCC AAGAGAAGGG CAGTGGTGGT CATACAGGCA 
CATGCCAGGG GCATGGCTGC GCGGCGGAAC TTCCAGCAGC AGAAAGCCAA TGGGCCTCTG 
GTCATCCCAG CTGAAGGGCA GAAGAGCCAA AGTATTCTCC CCACCAGGAA GCGTAAGTCA 
ATCTACGATA CCATCACTGA CACGGAGATG GTGGAGCAAG TGTTTGGCTT CCTCCCTTCC 
ATGATTGGTG GGCAGGAGGG CCAGGCTCCA TGGCGCTTTG AGGACCTGGA AGCGAAGAGC 
CAGAAGCTGC CCGAGGTTGA CCTGGACACG GTCCCCCTGG TGGAGGAGTC TGAGGACGAT 
GTGGACGGGC TGGCCGAGTA CACCTTCCCC AAGTTTGCTG CCACCTACTT CCAGAAATCG 
GCCAGCCACA CGCACATCCG GCGGCCCCTC CGATACCCAC TGCTCTACCA TGAGGAAGAT 
GCTGACTGCT CGGCCGCCCT GGCCGTGTGG AACATCATTC TGAGATTCAT GGGTGACCTT 
CCAGAGCCAG TGCTTTTTGC CAGGAATGGC CCGCCCGGCG GCTCCGTGAC GCGGCAGATC 
CGTGACAGCC TGGGCAGGGA GGGCGGCACC CGGTCCCCAC CGCACTGCGG GTCCACACAG 
AAGTCCAGCC GCAGAGACAG AGGGAGCAAG GACATCTCCT CCATGAAGCT AAAGCGGTCC 
TCCAAGATCA CAAGCCAGGT AACCAGCCAG CTGAATATTG GAGAGGAGGC ATTTGAGCCT 
GATGGCCCCA TCTCAGACCG ACCCATGTCC AATCTGGAGA AGGTACACTT CATTGTGGGC 
TATGCTATCC TGCGGCCTAA CCTCAGGGAC GAGATTTACT GCCAGATCTG CAAGCAGCTG 
TCCGAGAACT TCAAAACAAG CTGCCTGGCC CGGGGCTGGA TCCTGCTCAG CCTTTGCTTG 
GGCTGTTTCC CACCCTCCGA GAGGTTCATG AAGTATCTGC TAAACTTCAT TGGTCAAGGG 
CCAGCTGGCT ATGGGCCCTT CTGTGCAGAA CGCCTGAGAC GAACCTATGC TAATGGGGTA 
CGCACGGAGC CCCCCACGTG GCTGGAGCTG CAGGCAGTCA AATCCAAGAA GCACATCCCA 
ATCCATGTCA TCTTGGTGAC CGGAGAGAGC CTGACTGTCA TGGTTGACTC TGCCTCGACA 
TCTCGGGAAG TCTGCCTGCA CATTGCCCAC AAGCAGGGCC TCAGCGATCA CCTGGGCTTT 
TCTCTCCAGG TGGCCGTGTA CGACAAGTTC TGGTCCCTGG GCAGCGGGCG CGACCACGTG 
ATGGATACAG TCGCCCAGTG TGAGCAGCTG GCCCGGGACA GGGGGGAGAA CGAGCGCCAG 
TCGCCCTGGC GCATCTACTT CCGGAAGGAG TTCTTCACCC CCTGGCATGA CTCCCAGGAG 
GACCCCGTCA GTACCCAGCT CATCTACCGC CAAGTGCTGC ATGGGGTCTG GTCTGGCGAG 
TACAACTTCG AGAAGGAGGA TGATCTGGTA CAGCTGCTGG CCAAGCACTG CTACGTGCAG 
CTCGGTGCCT CGGTGGGGAG TGAAGCTGTC CAGGAGCTGC TGCCCAGCTG CATCCCCTCC 
AAGCTGTACA AGACCAAGCC TCCAGAGAAG TGGGCTAGCC TCGTCACTGC TGCCCACGCC 
AAGGCACAGT ACACCCAGAA GCAGGCAAAG CCGCTGGCTG TGCAGGAGCA GGTGGTGGAC 
ACGGCCCGCC TGCAGTGGCC GCTGCTCTTC TCCCGGCTCT TTGAGGTCAC CACGCTCTCC 
GGCCCCCGAC TGCCCAAGAC TCAGCTGATC TTAGCTGTTA ACTGGAAGGG GATGTACTTC 
CTGGACCAGA GAGAGAAGAT ACTCCTAGAA CTCTCTTTTC CAGAGGTCGT GAGCCTGATC 
ACCAACAGAG AGGCGCAGGG TGGGCAGAGG CTGGTACTCT CCACGCTGCA CGAAGAGGAG 
TACAAGTTCA TGTCCCCCAG CAGCGTGGCC ATCGCCGAGC TGGTAGCTAT GTTTCTGGAA 
GGCTTGAAGG AGAGGTCCGT GTTCGCCATG GCTCTGCAGA ACAGGAAGGC CACAGATGAC 
ACCACCATCC TGCCCTTCAA GAAGGGGGAC CTGCTGATCC TGACAAAGAA GCAGGGGTTG 
TTGGCCTCTG AGAACTGGAC CTTGGGCCAG AACGACAGGA CAGGCAAGAC GGGGCTGGTG 
CCCACAGCTT GCCTCTACAC CATCCCAACG GTCACCAAGC CCTCGGCACA GCTGATGAGT 
TTGCTGGCCA TGTCACCAGA GAAGCGGAAG CTGGCAGCCC AGGAGGTGCA GCCCACAGAG 
CCACTGCCTG AGGAGCAGCC CAAGGAGAAG CCACACACCC TGGAGGAATT CTCCTACGAG 
TTCTTTAGGG CCCCAGAGAA GGAGACAGTC AGGAGGACCA TGTTGCCCCT GGCCCGGACA 
CAGGGCCACC TGTGGGCCCA CTCATCCGAG CCACTGCGGC AGCCTCTGCT CAAGCAAGTC 
CACGCCAATG CCGAGCTCCG GGACCCTGCC TGTCAGATCT TCATTGGCAT CCTCCGGTAC 
ATGGGTGACT ACCCATCTCG ACAGGCTTGG ACCTCCGTGG AGCTCGCCGA CCAGATCTTC 
TCGTTGGCCC TCCAGGAGTC AGCCCTGCGG GATGAGGTCT ACTGCCAGAT CCTGAAGCAG 
CTGACACACA ACACCACGAG GTACAGTGAG GAGCGGGGCT GGCAGCTGCT GTGGCTCTGC 
ACTGGCCTCT TCCCCCCGGG AAAGGCGCTG CTGCCTCATG CTCAGAAGTT CGTAGACACG 
CGGAGGAAGA AGCTATTGGC TCCCGACTGC AGCCGGCGCA TCCAGAGGAT CCTGAGGACA 
GGACCCCGGA AGCAGCCCCC ACACCCAGTG GAGGTGGAGG CCATAGAGCA GAACATCTCT 
CGCATCTGCC ACAAGATCTA CTTCCCTAAC GACACTAGCG AGATGCTGGA GGTGGGCACC 
TACACTCGGG TGCGAGATGT ATGCCAGGGC ATCGCCACCA GGCTGCAGCT GTCTTCCTGG 
GAAGGCTGCA GCCTCTTCAT CAAGATTGCT GACAAGGTCA TCAGCCAGAA GGAGGGAGAC 
TTCTTCTTTG ACTCCTTGCG GCAAGTGTCT GATTGGGTGA AGAAAAACAA GCCCCAGAAA 
GAAGGGGCGC CCGTGACACT CCCCTACCAG GTGTACTTCA TGCGGAAATT GTGGCTCAAC 
GTGACCCCGG GGAAGGATGT GAATGCAGAC ACCGTACTTC ATTACCATCA GGAGCTGCCC 
AAGTACCTTC GTGGATTCCA CAAGTGTTCA CAGGAGGATG CTGTCCACCT GGCCGGCCTC 
ATCTACAAGG CCCAGTTTGA CAACAACCGG TCCCAGCTAG CTAGTATCCC CAAGATCCTG 
AGGGAGCTTG TGCCTGAGAA CCTGACACGT CTCATGTCCT CCGAGGAATG GAGAAAGAAC 
ATCCTTCTGG CCTATGACAA GCACAAGGAC AAGACAGTAG AGGAGGCCAA GGTGGCGTTC 
CTCAAGGGGA TCTGCCGGTG GCCCACATTT GGGTCTGCCT TCTTCGAGGT GAAGCAAACC 
TCGGAGCCTT CCTACCCAGA CATCATCCTC ATCGCCATCA ACCGGCACGG GGTTCTGCTT 
ATCCACCCCA AGACCAAGGA GCTGCTCACC ACCTACCCCT TCACCAAGAT CTCCAGCTGG 
AGCAGCGGCA GCACCTACTT CCACATGGCA CTGGGGAGCC TGGCCCGGGG CAGCCGCCTG 
CTGTGTGAAA CCTCTCTGGG CTACAAGATG GATGACCTGC TGACCTCGTA TGTGCAGCAG 
CTCCTGAGCG CCATGAACAA GCAGCGGGGC TCCCAGACCC CGGCCCTGGC TGATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022261717.1
CDS	247..5826
Translation	MFARLSGTPAPPGSRGAELPHLLRHAPGDDRGGEEAAEPGDALRVPLPDHAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKELLTTYPFTKISSWSSGSTYFHMALGSLARGSRLLCETSLGYKMDDLLTSYVQQLLSAMNKQRGSQTPALADP

Target ORF information:

RefSeq Version	XM_022406009.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406009.1

ORF Insert Sequence:

ATGTTTGCCA GGCTCTCTGG AACACCTGCC CCTCCAGGCT CCCGAGGAGC GGAACTACCA 
CATCTTCTAC GGCATGCTCC TGGGGATGAC CGCGGAGGAG AAGAAGCTGC TGAGCCTGGG 
GACGCCCTCC GAGTACCACT ACCTGACCAC GCTGCAGTCT TCGAGAACCT GGATTCCTCC 
GACGTGATGG AGACGCCTGC CTTTCCCACC GTGCTGAAGT TACTGGAGGT GGAGTACCAG 
GCACTCCGGG ACTGTCTGAT CAAGCATTCC ATCATCATCC GAGGGGAGTT TGTCACCAGG 
CCCCTGAACA TCACTCAGGC TGCAGACCGG AGGGATGCCT TTGTCAAGGG CATCTATGGG 
CACCTCTTCC TGTGGATTGT CAAGAAGATC AACACTGCAA TTTTCACTCC ACCTGCCCAG 
GACCCCAAAA ATGTGCGAAG AGCCATTGGC CTCCTGGACA TATTTGGCTT TGAAAATTTC 
CAGAACAATA GCTTCGAGCA GCTCTGCATC AACTTTGCCA ACGAGCACCT GCAGCAGTTC 
TTCGTGCAGC ATGTGTTCAC CATGGAGCAG GAGGAGTACC TCTCAGAGAA CATTGCCTGG 
GACTACATCC ACTACAATGA CAATCGGCCC ACCCTGGATC TGCTGGCCCT TAAGCCCATG 
AGCATCATCT CCCTCCTGGA CGAGGAGAGC CACTTCCCAC AGGGGACAGA TACAACCATG 
CTGCAGAAAC TGAACAGTGT CCATGCCAAC AATAAGGCCT ACCTACAGCC CAGGAACATC 
CATGATGCCA GGTTTGGCAT CGCCCACTTT GCAGGCGAGG TGTACTACCA GACAGAAGGC 
TTCCTGGAGA AGAATCGAGA CATGCTGAGC ACAGATATCC TCACTCTGGT CTACTCCTCC 
AAAAACAAGT TCCTGAAGGA GATATTCAAG CTGGAGTCAG CGGGGACCAA GCTGGGCCAA 
AGCACCATCA TCCAGGCTAG CGCAGGGAGC CAGCTCTTCA AGTCTGCAGA CTCCAGCAAG 
CAGCCCTCCA CATTGGCAGG CCAGTTCAAG AAGTCCCTGG ACCAGCTGAT GAAAATCCTG 
ACCAGCTGCC AGCCCTACTT TATCCGCTGC ATCAAACCCA ATGAGTACAA GAAGCCACTG 
CTCTTCGACC GCGACCTGTG CCTCCGGCAG CTGCGTTACT CGGGGATGAT GGAGACCGTG 
CACATCCGCA GGTCCGGCTT CCCCATCCGG TACACGTTCC AGGAGTTCGC GCAGAGGTTC 
GGCGTGCTGC TGCCCAGCGC CGTTCGCCTG CAGCTTCGAG ACAAGGCTCG CCAGATGACC 
CTGCGCATTG CTGAAATGCA GCTGGGGACG GACAAAGAAT GGAAAGTGGG AAAGACCAAA 
ATTTTCCTGA AGGATAACCA GGACACTCTA CTTGAGGTAC AGAGGACCCA GACCCTGGAC 
AAGGCAGCAA TCAACATCCA AAGAGTACTG CGAGGCTACA AATACAGGAA GGAGTTCTTG 
AGACAGAGGC GGGCCGCTGT GACCCTCCAG GCCAGGTGGA GAGGCTACTA CAACAAGCGG 
AACTTCAAGC AGATCCTCTT GGGTTTTGAG CGCCTGCAGG CCATTGCCCG GAGCCAGTGG 
CTAGCAAAGC AGTACCAGAC CATGCGGCAG AGGATGGTCC AGCTGCAGGC ACTCTGCAGG 
GGCTACCTTG TGCGCCAGCA AGTCCAGGCC AAGAGAAGGG CAGTGGTGGT CATACAGGCA 
CATGCCAGGG GCATGGCTGC GCGGCGGAAC TTCCAGCAGC AGAAAGCCAA TGGGCCTCTG 
GTCATCCCAG CTGAAGGGCA GAAGAGCCAA AGTATTCTCC CCACCAGGAA GCGTAAGTCA 
ATCTACGATA CCATCACTGA CACGGAGATG GTGGAGCAAG TGTTTGGCTT CCTCCCTTCC 
ATGATTGGTG GGCAGGAGGG CCAGGCTCCA TGGCGCTTTG AGGACCTGGA AGCGAAGAGC 
CAGAAGCTGC CCGAGGTTGA CCTGGACACG GTCCCCCTGG TGGAGGAGTC TGAGGACGAT 
GTGGACGGGC TGGCCGAGTA CACCTTCCCC AAGTTTGCTG CCACCTACTT CCAGAAATCG 
GCCAGCCACA CGCACATCCG GCGGCCCCTC CGATACCCAC TGCTCTACCA TGAGGAAGAT 
GCTGACTGCT CGGCCGCCCT GGCCGTGTGG AACATCATTC TGAGATTCAT GGGTGACCTT 
CCAGAGCCAG TGCTTTTTGC CAGGAATGGC CCGCCCGGCG GCTCCGTGAC GCGGCAGATC 
CGTGACAGCC TGGGCAGGGA GGGCGGCACC CGGTCCCCAC CGCACTGCGG GTCCACACAG 
AAGTCCAGCC GCAGAGACAG AGGGAGCAAG GACATCTCCT CCATGAAGCT AAAGCGGTCC 
TCCAAGATCA CAAGCCAGGT AACCAGCCAG CTGAATATTG GAGAGGAGGC ATTTGAGCCT 
GATGGCCCCA TCTCAGACCG ACCCATGTCC AATCTGGAGA AGGTACACTT CATTGTGGGC 
TATGCTATCC TGCGGCCTAA CCTCAGGGAC GAGATTTACT GCCAGATCTG CAAGCAGCTG 
TCCGAGAACT TCAAAACAAG CTGCCTGGCC CGGGGCTGGA TCCTGCTCAG CCTTTGCTTG 
GGCTGTTTCC CACCCTCCGA GAGGTTCATG AAGTATCTGC TAAACTTCAT TGGTCAAGGG 
CCAGCTGGCT ATGGGCCCTT CTGTGCAGAA CGCCTGAGAC GAACCTATGC TAATGGGGTA 
CGCACGGAGC CCCCCACGTG GCTGGAGCTG CAGGCAGTCA AATCCAAGAA GCACATCCCA 
ATCCATGTCA TCTTGGTGAC CGGAGAGAGC CTGACTGTCA TGGTTGACTC TGCCTCGACA 
TCTCGGGAAG TCTGCCTGCA CATTGCCCAC AAGCAGGGCC TCAGCGATCA CCTGGGCTTT 
TCTCTCCAGG TGGCCGTGTA CGACAAGTTC TGGTCCCTGG GCAGCGGGCG CGACCACGTG 
ATGGATACAG TCGCCCAGTG TGAGCAGCTG GCCCGGGACA GGGGGGAGAA CGAGCGCCAG 
TCGCCCTGGC GCATCTACTT CCGGAAGGAG TTCTTCACCC CCTGGCATGA CTCCCAGGAG 
GACCCCGTCA GTACCCAGCT CATCTACCGC CAAGTGCTGC ATGGGGTCTG GTCTGGCGAG 
TACAACTTCG AGAAGGAGGA TGATCTGGTA CAGCTGCTGG CCAAGCACTG CTACGTGCAG 
CTCGGTGCCT CGGTGGGGAG TGAAGCTGTC CAGGAGCTGC TGCCCAGCTG CATCCCCTCC 
AAGCTGTACA AGACCAAGCC TCCAGAGAAG TGGGCTAGCC TCGTCACTGC TGCCCACGCC 
AAGGCACAGT ACACCCAGAA GCAGGCAAAG CCGCTGGCTG TGCAGGAGCA GGTGGTGGAC 
ACGGCCCGCC TGCAGTGGCC GCTGCTCTTC TCCCGGCTCT TTGAGGTCAC CACGCTCTCC 
GGCCCCCGAC TGCCCAAGAC TCAGCTGATC TTAGCTGTTA ACTGGAAGGG GATGTACTTC 
CTGGACCAGA GAGAGAAGAT ACTCCTAGAA CTCTCTTTTC CAGAGGTCGT GAGCCTGATC 
ACCAACAGAG AGGCGCAGGG TGGGCAGAGG CTGGTACTCT CCACGCTGCA CGAAGAGGAG 
TACAAGTTCA TGTCCCCCAG CAGCGTGGCC ATCGCCGAGC TGGTAGCTAT GTTTCTGGAA 
GGCTTGAAGG AGAGGTCCGT GTTCGCCATG GCTCTGCAGA ACAGGAAGGC CACAGATGAC 
ACCACCATCC TGCCCTTCAA GAAGGGGGAC CTGCTGATCC TGACAAAGAA GCAGGGGTTG 
TTGGCCTCTG AGAACTGGAC CTTGGGCCAG AACGACAGGA CAGGCAAGAC GGGGCTGGTG 
CCCACAGCTT GCCTCTACAC CATCCCAACG GTCACCAAGC CCTCGGCACA GCTGATGAGT 
TTGCTGGCCA TGTCACCAGA GAAGCGGAAG CTGGCAGCCC AGGAGGTGCA GCCCACAGAG 
CCACTGCCTG AGGAGCAGCC CAAGGAGAAG CCACACACCC TGGAGGAATT CTCCTACGAG 
TTCTTTAGGG CCCCAGAGAA GGAGACAGTC AGGAGGACCA TGTTGCCCCT GGCCCGGACA 
CAGGGCCACC TGTGGGCCCA CTCATCCGAG CCACTGCGGC AGCCTCTGCT CAAGCAAGTC 
CACGCCAATG CCGAGCTCCG GGACCCTGCC TGTCAGATCT TCATTGGCAT CCTCCGGTAC 
ATGGGTGACT ACCCATCTCG ACAGGCTTGG ACCTCCGTGG AGCTCGCCGA CCAGATCTTC 
TCGTTGGCCC TCCAGGAGTC AGCCCTGCGG GATGAGGTCT ACTGCCAGAT CCTGAAGCAG 
CTGACACACA ACACCACGAG GTACAGTGAG GAGCGGGGCT GGCAGCTGCT GTGGCTCTGC 
ACTGGCCTCT TCCCCCCGGG AAAGGCGCTG CTGCCTCATG CTCAGAAGTT CGTAGACACG 
CGGAGGAAGA AGCTATTGGC TCCCGACTGC AGCCGGCGCA TCCAGAGGAT CCTGAGGACA 
GGACCCCGGA AGCAGCCCCC ACACCCAGTG GAGGTGGAGG CCATAGAGCA GAACATCTCT 
CGCATCTGCC ACAAGATCTA CTTCCCTAAC GACACTAGCG AGATGCTGGA GGTGGGCACC 
TACACTCGGG TGCGAGATGT ATGCCAGGGC ATCGCCACCA GGCTGCAGCT GTCTTCCTGG 
GAAGGCTGCA GCCTCTTCAT CAAGATTGCT GACAAGGTCA TCAGCCAGAA GGAGGGAGAC 
TTCTTCTTTG ACTCCTTGCG GCAAGTGTCT GATTGGGTGA AGAAAAACAA GCCCCAGAAA 
GAAGGGGCGC CCGTGACACT CCCCTACCAG GTGTACTTCA TGCGGAAATT GTGGCTCAAC 
GTGACCCCGG GGAAGGATGT GAATGCAGAC ACCGTACTTC ATTACCATCA GGAGCTGCCC 
AAGTACCTTC GTGGATTCCA CAAGTGTTCA CAGGAGGATG CTGTCCACCT GGCCGGCCTC 
ATCTACAAGG CCCAGTTTGA CAACAACCGG TCCCAGCTAG CTAGTATCCC CAAGATCCTG 
AGGGAGCTTG TGCCTGAGAA CCTGACACGT CTCATGTCCT CCGAGGAATG GAGAAAGAAC 
ATCCTTCTGG CCTATGACAA GCACAAGGAC AAGACAGTAG AGGAGGCCAA GGTGGCGTTC 
CTCAAGGGGA TCTGCCGGTG GCCCACATTT GGGTCTGCCT TCTTCGAGGT GAAGCAAACC 
TCGGAGCCTT CCTACCCAGA CATCATCCTC ATCGCCATCA ACCGGCACGG GGTTCTGCTT 
ATCCACCCCA AGACCAAGGA GCTGCTCACC ACCTACCCCT TCACCAAGAT CTCCAGCTGG 
AGCAGCGGCA GCACCTACTT CCACATGGCA CTGGGGAGCC TGGCCCGGGG CAGCCGCCTG 
CTGTGTGAAA CCTCTCTGGG CTACAAGATG GATGACCTGC TGACCTCGTA TGTGCAGCAG 
CTCCTGAGCG CCATGAACAA GCAGCGGGGC TCCCAGACCC CGGCCCTGGC TGATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_022406008.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022261716.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.

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CloneID	OCe295083
Clone ID Related Accession (Same CDS sequence)	XM_022406008.1
Accession Version	XM_022406008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	unconventional myosin-VIIb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCT TCAGGCTGGG TGATCATGTG TGGCTAAACC CCCTCTCTGC CAACAAGACC 
AGTGTGGCCA TTGGATCCAT TGTCAAGGAA ACAAAGCCTG GCAAGATCTT GGTTGTAGAT 
GATGAAGGCA AGGAACACTG GATCCAAGCA AAAGACCTTG ACACCCTCAG CCCCATGCAC 
CCCAACTCAG TGCAAGGCGT GGATGACATG ATCCGCCTGG GGGAACTGAA CGAGGCAGGC 
ATGGTGCACA ACCTCCTGAT CCGCTACCGG CAGCACAAGA TCTATACATA CACAGGCTCC 
ATTCTGATGG CCGTGAACCC CTTCCAAGTG TTGCCCCTCT ACACCCTGGA GCAGGTACAG 
CTGTACTACA ACCGTCACAT GGGCGAACTA CCACCCCATG TCTTTGCCAT CGCCAACAAC 
TGCTACTTCA ACATGAAGAG GAACAAGAGG GACCAGTGCT GCATTATCAG TGGTGAGTCT 
GGGGCAGGGA AGACAGAGAC CACGAAGCTC ATCCTGCAGT TCCTGGCCAC CGTCAGTGGC 
CAACATTCCT GGATTGAGCA GCAGGTCTTG GAGGCCAACC CCATCCTGGA AGCCTTCGGA 
AATGCCAAGA CAGTCAGAAA TGATAACTCA AGCCGCTTTG GGAAATATAT CGACATCCAC 
TTCAACCCCA ATGGGGTGAT TGAGGGCGCA CGCATCGAGC AATTTCTCCT GGAGAAGTCC 
CGGGTCTGCC GGCAGGCTCC CGAGGAGCGG AACTACCACA TCTTCTACGG CATGCTCCTG 
GGGATGACCG CGGAGGAGAA GAAGCTGCTG AGCCTGGGGA CGCCCTCCGA GTACCACTAC 
CTGACCACGG GAAACTGCAC ATCCTGTGAG GGCCTCAATG ATGCCAAGGA CTATGCCCAT 
GTTCGCTCTG CCATGAAGAT CCTCATGTTC TCCGACTCTG AGAACTGGGA CCTCAGCAAG 
CTGCTGGCAG CCATTCTCCA CCTGGGCAAT GTGGAGTTTA TGGCTGCAGT CTTCGAGAAC 
CTGGATTCCT CCGACGTGAT GGAGACGCCT GCCTTTCCCA CCGTGCTGAA GTTACTGGAG 
GTGGAGTACC AGGCACTCCG GGACTGTCTG ATCAAGCATT CCATCATCAT CCGAGGGGAG 
TTTGTCACCA GGCCCCTGAA CATCACTCAG GCTGCAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCACCTCTT CCTGTGGATT GTCAAGAAGA TCAACACTGC AATTTTCACT 
CCACCTGCCC AGGACCCCAA AAATGTGCGA AGAGCCATTG GCCTCCTGGA CATATTTGGC 
TTTGAAAATT TCCAGAACAA TAGCTTCGAG CAGCTCTGCA TCAACTTTGC CAACGAGCAC 
CTGCAGCAGT TCTTCGTGCA GCATGTGTTC ACCATGGAGC AGGAGGAGTA CCTCTCAGAG 
AACATTGCCT GGGACTACAT CCACTACAAT GACAATCGGC CCACCCTGGA TCTGCTGGCC 
CTTAAGCCCA TGAGCATCAT CTCCCTCCTG GACGAGGAGA GCCACTTCCC ACAGGGGACA 
GATACAACCA TGCTGCAGAA ACTGAACAGT GTCCATGCCA ACAATAAGGC CTACCTACAG 
CCCAGGAACA TCCATGATGC CAGGTTTGGC ATCGCCCACT TTGCAGGCGA GGTGTACTAC 
CAGACAGAAG GCTTCCTGGA GAAGAATCGA GACATGCTGA GCACAGATAT CCTCACTCTG 
GTCTACTCCT CCAAAAACAA GTTCCTGAAG GAGATATTCA AGCTGGAGTC AGCGGGGACC 
AAGCTGGGCC AAAGCACCAT CATCCAGGCT AGCGCAGGGA GCCAGCTCTT CAAGTCTGCA 
GACTCCAGCA AGCAGCCCTC CACATTGGCA GGCCAGTTCA AGAAGTCCCT GGACCAGCTG 
ATGAAAATCC TGACCAGCTG CCAGCCCTAC TTTATCCGCT GCATCAAACC CAATGAGTAC 
AAGAAGCCAC TGCTCTTCGA CCGCGACCTG TGCCTCCGGC AGCTGCGTTA CTCGGGGATG 
ATGGAGACCG TGCACATCCG CAGGTCCGGC TTCCCCATCC GGTACACGTT CCAGGAGTTC 
GCGCAGAGGT TCGGCGTGCT GCTGCCCAGC GCCGTTCGCC TGCAGCTTCG AGACAAGGCT 
CGCCAGATGA CCCTGCGCAT TGCTGAAATG CAGCTGGGGA CGGACAAAGA ATGGAAAGTG 
GGAAAGACCA AAATTTTCCT GAAGGATAAC CAGGACACTC TACTTGAGGT ACAGAGGACC 
CAGACCCTGG ACAAGGCAGC AATCAACATC CAAAGAGTAC TGCGAGGCTA CAAATACAGG 
AAGGAGTTCT TGAGACAGAG GCGGGCCGCT GTGACCCTCC AGGCCAGGTG GAGAGGCTAC 
TACAACAAGC GGAACTTCAA GCAGATCCTC TTGGGTTTTG AGCGCCTGCA GGCCATTGCC 
CGGAGCCAGT GGCTAGCAAA GCAGTACCAG ACCATGCGGC AGAGGATGGT CCAGCTGCAG 
GCACTCTGCA GGGGCTACCT TGTGCGCCAG CAAGTCCAGG CCAAGAGAAG GGCAGTGGTG 
GTCATACAGG CACATGCCAG GGGCATGGCT GCGCGGCGGA ACTTCCAGCA GCAGAAAGCC 
AATGGGCCTC TGGTCATCCC AGCTGAAGGG CAGAAGAGCC AAAGTATTCT CCCCACCAGG 
AAGCGTAAGT CAATCTACGA TACCATCACT GACACGGAGA TGGTGGAGCA AGTGTTTGGC 
TTCCTCCCTT CCATGATTGG TGGGCAGGAG GGCCAGGCTC CATGGCGCTT TGAGGACCTG 
GAAGCGAAGA GCCAGAAGCT GCCCGAGGTT GACCTGGACA CGGTCCCCCT GGTGGAGGAG 
TCTGAGGACG ATGTGGACGG GCTGGCCGAG TACACCTTCC CCAAGTTTGC TGCCACCTAC 
TTCCAGAAAT CGGCCAGCCA CACGCACATC CGGCGGCCCC TCCGATACCC ACTGCTCTAC 
CATGAGGAAG ATGCTGACTG CTCGGCCGCC CTGGCCGTGT GGAACATCAT TCTGAGATTC 
ATGGGTGACC TTCCAGAGCC AGTGCTTTTT GCCAGGAATG GCCCGCCCGG CGGCTCCGTG 
ACGCGGCAGA TCCGTGACAG CCTGGGCAGG GAGGGCGGCA CCCGGTCCCC ACCGCACTGC 
GGGTCCACAC AGAAGTCCAG CCGCAGAGAC AGAGGGAGCA AGGACATCTC CTCCATGAAG 
CTAAAGCGGT CCTCCAAGAT CACAAGCCAG GTAACCAGCC AGCTGAATAT TGGAGAGGAG 
GCATTTGAGC CTGATGGCCC CATCTCAGAC CGACCCATGT CCAATCTGGA GAAGGTACAC 
TTCATTGTGG GCTATGCTAT CCTGCGGCCT AACCTCAGGG ACGAGATTTA CTGCCAGATC 
TGCAAGCAGC TGTCCGAGAA CTTCAAAACA AGCTGCCTGG CCCGGGGCTG GATCCTGCTC 
AGCCTTTGCT TGGGCTGTTT CCCACCCTCC GAGAGGTTCA TGAAGTATCT GCTAAACTTC 
ATTGGTCAAG GGCCAGCTGG CTATGGGCCC TTCTGTGCAG AACGCCTGAG ACGAACCTAT 
GCTAATGGGG TACGCACGGA GCCCCCCACG TGGCTGGAGC TGCAGGCAGT CAAATCCAAG 
AAGCACATCC CAATCCATGT CATCTTGGTG ACCGGAGAGA GCCTGACTGT CATGGTTGAC 
TCTGCCTCGA CATCTCGGGA AGTCTGCCTG CACATTGCCC ACAAGCAGGG CCTCAGCGAT 
CACCTGGGCT TTTCTCTCCA GGTGGCCGTG TACGACAAGT TCTGGTCCCT GGGCAGCGGG 
CGCGACCACG TGATGGATAC AGTCGCCCAG TGTGAGCAGC TGGCCCGGGA CAGGGGGGAG 
AACGAGCGCC AGTCGCCCTG GCGCATCTAC TTCCGGAAGG AGTTCTTCAC CCCCTGGCAT 
GACTCCCAGG AGGACCCCGT CAGTACCCAG CTCATCTACC GCCAAGTGCT GCATGGGGTC 
TGGTCTGGCG AGTACAACTT CGAGAAGGAG GATGATCTGG TACAGCTGCT GGCCAAGCAC 
TGCTACGTGC AGCTCGGTGC CTCGGTGGGG AGTGAAGCTG TCCAGGAGCT GCTGCCCAGC 
TGCATCCCCT CCAAGCTGTA CAAGACCAAG CCTCCAGAGA AGTGGGCTAG CCTCGTCACT 
GCTGCCCACG CCAAGGCACA GTACACCCAG AAGCAGGCAA AGCCGCTGGC TGTGCAGGAG 
CAGGTGGTGG ACACGGCCCG CCTGCAGTGG CCGCTGCTCT TCTCCCGGCT CTTTGAGGTC 
ACCACGCTCT CCGGCCCCCG ACTGCCCAAG ACTCAGCTGA TCTTAGCTGT TAACTGGAAG 
GGGATGTACT TCCTGGACCA GAGAGAGAAG ATACTCCTAG AACTCTCTTT TCCAGAGGTC 
GTGAGCCTGA TCACCAACAG AGAGGCGCAG GGTGGGCAGA GGCTGGTACT CTCCACGCTG 
CACGAAGAGG AGTACAAGTT CATGTCCCCC AGCAGCGTGG CCATCGCCGA GCTGGTAGCT 
ATGTTTCTGG AAGGCTTGAA GGAGAGGTCC GTGTTCGCCA TGGCTCTGCA GAACAGGAAG 
GCCACAGATG ACACCACCAT CCTGCCCTTC AAGAAGGGGG ACCTGCTGAT CCTGACAAAG 
AAGCAGGGGT TGTTGGCCTC TGAGAACTGG ACCTTGGGCC AGAACGACAG GACAGGCAAG 
ACGGGGCTGG TGCCCACAGC TTGCCTCTAC ACCATCCCAA CGGTCACCAA GCCCTCGGCA 
CAGCTGATGA GTTTGCTGGC CATGTCACCA GAGAAGCGGA AGCTGGCAGC CCAGGAGGTG 
CAGCCCACAG AGCCACTGCC TGAGGAGCAG CCCAAGGAGA AGCCACACAC CCTGGAGGAA 
TTCTCCTACG AGTTCTTTAG GGCCCCAGAG AAGGAGACAG TCAGGAGGAC CATGTTGCCC 
CTGGCCCGGA CACAGGGCCA CCTGTGGGCC CACTCATCCG AGCCACTGCG GCAGCCTCTG 
CTCAAGCAAG TCCACGCCAA TGCCGAGCTC CGGGACCCTG CCTGTCAGAT CTTCATTGGC 
ATCCTCCGGT ACATGGGTGA CTACCCATCT CGACAGGCTT GGACCTCCGT GGAGCTCGCC 
GACCAGATCT TCTCGTTGGC CCTCCAGGAG TCAGCCCTGC GGGATGAGGT CTACTGCCAG 
ATCCTGAAGC AGCTGACACA CAACACCACG AGGTACAGTG AGGAGCGGGG CTGGCAGCTG 
CTGTGGCTCT GCACTGGCCT CTTCCCCCCG GGAAAGGCGC TGCTGCCTCA TGCTCAGAAG 
TTCGTAGACA CGCGGAGGAA GAAGCTATTG GCTCCCGACT GCAGCCGGCG CATCCAGAGG 
ATCCTGAGGA CAGGACCCCG GAAGCAGCCC CCACACCCAG TGGAGGTGGA GGCCATAGAG 
CAGAACATCT CTCGCATCTG CCACAAGATC TACTTCCCTA ACGACACTAG CGAGATGCTG 
GAGGTGGGCA CCTACACTCG GGTGCGAGAT GTATGCCAGG GCATCGCCAC CAGGCTGCAG 
CTGTCTTCCT GGGAAGGCTG CAGCCTCTTC ATCAAGATTG CTGACAAGGT CATCAGCCAG 
AAGGAGGGAG ACTTCTTCTT TGACTCCTTG CGGCAAGTGT CTGATTGGGT GAAGAAAAAC 
AAGCCCCAGA AAGAAGGGGC GCCCGTGACA CTCCCCTACC AGGTGTACTT CATGCGGAAA 
TTGTGGCTCA ACGTGACCCC GGGGAAGGAT GTGAATGCAG ACACCGTACT TCATTACCAT 
CAGGAGCTGC CCAAGTACCT TCGTGGATTC CACAAGTGTT CACAGGAGGA TGCTGTCCAC 
CTGGCCGGCC TCATCTACAA GGCCCAGTTT GACAACAACC GGTCCCAGCT AGCTAGTATC 
CCCAAGATCC TGAGGGAGCT TGTGCCTGAG AACCTGACAC GTCTCATGTC CTCCGAGGAA 
TGGAGAAAGA ACATCCTTCT GGCCTATGAC AAGCACAAGG ACAAGACAGT AGAGGAGGCC 
AAGGTGGCGT TCCTCAAGGG GATCTGCCGG TGGCCCACAT TTGGGTCTGC CTTCTTCGAG 
GTGAAGCAAA CCTCGGAGCC TTCCTACCCA GACATCATCC TCATCGCCAT CAACCGGCAC 
GGGGTTCTGC TTATCCACCC CAAGACCAAG GAGCTGCTCA CCACCTACCC CTTCACCAAG 
ATCTCCAGCT GGAGCAGCGG CAGCACCTAC TTCCACATGG CACTGGGGAG CCTGGCCCGG 
GGCAGCCGCC TGCTGTGTGA AACCTCTCTG GGCTACAAGA TGGATGACCT GCTGACCTCG 
TATGTGCAGC AGCTCCTGAG CGCCATGAAC AAGCAGCGGG GCTCCCAGAC CCCGGCCCTG 
GCTGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022261716.1
CDS	283..6714
Translation	MSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKELLTTYPFTKISSWSSGSTYFHMALGSLARGSRLLCETSLGYKMDDLLTSYVQQLLSAMNKQRGSQTPALADP

Target ORF information:

RefSeq Version	XM_022406008.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406008.1

ORF Insert Sequence:

ATGTCAGCCT TCAGGCTGGG TGATCATGTG TGGCTAAACC CCCTCTCTGC CAACAAGACC 
AGTGTGGCCA TTGGATCCAT TGTCAAGGAA ACAAAGCCTG GCAAGATCTT GGTTGTAGAT 
GATGAAGGCA AGGAACACTG GATCCAAGCA AAAGACCTTG ACACCCTCAG CCCCATGCAC 
CCCAACTCAG TGCAAGGCGT GGATGACATG ATCCGCCTGG GGGAACTGAA CGAGGCAGGC 
ATGGTGCACA ACCTCCTGAT CCGCTACCGG CAGCACAAGA TCTATACATA CACAGGCTCC 
ATTCTGATGG CCGTGAACCC CTTCCAAGTG TTGCCCCTCT ACACCCTGGA GCAGGTACAG 
CTGTACTACA ACCGTCACAT GGGCGAACTA CCACCCCATG TCTTTGCCAT CGCCAACAAC 
TGCTACTTCA ACATGAAGAG GAACAAGAGG GACCAGTGCT GCATTATCAG TGGTGAGTCT 
GGGGCAGGGA AGACAGAGAC CACGAAGCTC ATCCTGCAGT TCCTGGCCAC CGTCAGTGGC 
CAACATTCCT GGATTGAGCA GCAGGTCTTG GAGGCCAACC CCATCCTGGA AGCCTTCGGA 
AATGCCAAGA CAGTCAGAAA TGATAACTCA AGCCGCTTTG GGAAATATAT CGACATCCAC 
TTCAACCCCA ATGGGGTGAT TGAGGGCGCA CGCATCGAGC AATTTCTCCT GGAGAAGTCC 
CGGGTCTGCC GGCAGGCTCC CGAGGAGCGG AACTACCACA TCTTCTACGG CATGCTCCTG 
GGGATGACCG CGGAGGAGAA GAAGCTGCTG AGCCTGGGGA CGCCCTCCGA GTACCACTAC 
CTGACCACGG GAAACTGCAC ATCCTGTGAG GGCCTCAATG ATGCCAAGGA CTATGCCCAT 
GTTCGCTCTG CCATGAAGAT CCTCATGTTC TCCGACTCTG AGAACTGGGA CCTCAGCAAG 
CTGCTGGCAG CCATTCTCCA CCTGGGCAAT GTGGAGTTTA TGGCTGCAGT CTTCGAGAAC 
CTGGATTCCT CCGACGTGAT GGAGACGCCT GCCTTTCCCA CCGTGCTGAA GTTACTGGAG 
GTGGAGTACC AGGCACTCCG GGACTGTCTG ATCAAGCATT CCATCATCAT CCGAGGGGAG 
TTTGTCACCA GGCCCCTGAA CATCACTCAG GCTGCAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCACCTCTT CCTGTGGATT GTCAAGAAGA TCAACACTGC AATTTTCACT 
CCACCTGCCC AGGACCCCAA AAATGTGCGA AGAGCCATTG GCCTCCTGGA CATATTTGGC 
TTTGAAAATT TCCAGAACAA TAGCTTCGAG CAGCTCTGCA TCAACTTTGC CAACGAGCAC 
CTGCAGCAGT TCTTCGTGCA GCATGTGTTC ACCATGGAGC AGGAGGAGTA CCTCTCAGAG 
AACATTGCCT GGGACTACAT CCACTACAAT GACAATCGGC CCACCCTGGA TCTGCTGGCC 
CTTAAGCCCA TGAGCATCAT CTCCCTCCTG GACGAGGAGA GCCACTTCCC ACAGGGGACA 
GATACAACCA TGCTGCAGAA ACTGAACAGT GTCCATGCCA ACAATAAGGC CTACCTACAG 
CCCAGGAACA TCCATGATGC CAGGTTTGGC ATCGCCCACT TTGCAGGCGA GGTGTACTAC 
CAGACAGAAG GCTTCCTGGA GAAGAATCGA GACATGCTGA GCACAGATAT CCTCACTCTG 
GTCTACTCCT CCAAAAACAA GTTCCTGAAG GAGATATTCA AGCTGGAGTC AGCGGGGACC 
AAGCTGGGCC AAAGCACCAT CATCCAGGCT AGCGCAGGGA GCCAGCTCTT CAAGTCTGCA 
GACTCCAGCA AGCAGCCCTC CACATTGGCA GGCCAGTTCA AGAAGTCCCT GGACCAGCTG 
ATGAAAATCC TGACCAGCTG CCAGCCCTAC TTTATCCGCT GCATCAAACC CAATGAGTAC 
AAGAAGCCAC TGCTCTTCGA CCGCGACCTG TGCCTCCGGC AGCTGCGTTA CTCGGGGATG 
ATGGAGACCG TGCACATCCG CAGGTCCGGC TTCCCCATCC GGTACACGTT CCAGGAGTTC 
GCGCAGAGGT TCGGCGTGCT GCTGCCCAGC GCCGTTCGCC TGCAGCTTCG AGACAAGGCT 
CGCCAGATGA CCCTGCGCAT TGCTGAAATG CAGCTGGGGA CGGACAAAGA ATGGAAAGTG 
GGAAAGACCA AAATTTTCCT GAAGGATAAC CAGGACACTC TACTTGAGGT ACAGAGGACC 
CAGACCCTGG ACAAGGCAGC AATCAACATC CAAAGAGTAC TGCGAGGCTA CAAATACAGG 
AAGGAGTTCT TGAGACAGAG GCGGGCCGCT GTGACCCTCC AGGCCAGGTG GAGAGGCTAC 
TACAACAAGC GGAACTTCAA GCAGATCCTC TTGGGTTTTG AGCGCCTGCA GGCCATTGCC 
CGGAGCCAGT GGCTAGCAAA GCAGTACCAG ACCATGCGGC AGAGGATGGT CCAGCTGCAG 
GCACTCTGCA GGGGCTACCT TGTGCGCCAG CAAGTCCAGG CCAAGAGAAG GGCAGTGGTG 
GTCATACAGG CACATGCCAG GGGCATGGCT GCGCGGCGGA ACTTCCAGCA GCAGAAAGCC 
AATGGGCCTC TGGTCATCCC AGCTGAAGGG CAGAAGAGCC AAAGTATTCT CCCCACCAGG 
AAGCGTAAGT CAATCTACGA TACCATCACT GACACGGAGA TGGTGGAGCA AGTGTTTGGC 
TTCCTCCCTT CCATGATTGG TGGGCAGGAG GGCCAGGCTC CATGGCGCTT TGAGGACCTG 
GAAGCGAAGA GCCAGAAGCT GCCCGAGGTT GACCTGGACA CGGTCCCCCT GGTGGAGGAG 
TCTGAGGACG ATGTGGACGG GCTGGCCGAG TACACCTTCC CCAAGTTTGC TGCCACCTAC 
TTCCAGAAAT CGGCCAGCCA CACGCACATC CGGCGGCCCC TCCGATACCC ACTGCTCTAC 
CATGAGGAAG ATGCTGACTG CTCGGCCGCC CTGGCCGTGT GGAACATCAT TCTGAGATTC 
ATGGGTGACC TTCCAGAGCC AGTGCTTTTT GCCAGGAATG GCCCGCCCGG CGGCTCCGTG 
ACGCGGCAGA TCCGTGACAG CCTGGGCAGG GAGGGCGGCA CCCGGTCCCC ACCGCACTGC 
GGGTCCACAC AGAAGTCCAG CCGCAGAGAC AGAGGGAGCA AGGACATCTC CTCCATGAAG 
CTAAAGCGGT CCTCCAAGAT CACAAGCCAG GTAACCAGCC AGCTGAATAT TGGAGAGGAG 
GCATTTGAGC CTGATGGCCC CATCTCAGAC CGACCCATGT CCAATCTGGA GAAGGTACAC 
TTCATTGTGG GCTATGCTAT CCTGCGGCCT AACCTCAGGG ACGAGATTTA CTGCCAGATC 
TGCAAGCAGC TGTCCGAGAA CTTCAAAACA AGCTGCCTGG CCCGGGGCTG GATCCTGCTC 
AGCCTTTGCT TGGGCTGTTT CCCACCCTCC GAGAGGTTCA TGAAGTATCT GCTAAACTTC 
ATTGGTCAAG GGCCAGCTGG CTATGGGCCC TTCTGTGCAG AACGCCTGAG ACGAACCTAT 
GCTAATGGGG TACGCACGGA GCCCCCCACG TGGCTGGAGC TGCAGGCAGT CAAATCCAAG 
AAGCACATCC CAATCCATGT CATCTTGGTG ACCGGAGAGA GCCTGACTGT CATGGTTGAC 
TCTGCCTCGA CATCTCGGGA AGTCTGCCTG CACATTGCCC ACAAGCAGGG CCTCAGCGAT 
CACCTGGGCT TTTCTCTCCA GGTGGCCGTG TACGACAAGT TCTGGTCCCT GGGCAGCGGG 
CGCGACCACG TGATGGATAC AGTCGCCCAG TGTGAGCAGC TGGCCCGGGA CAGGGGGGAG 
AACGAGCGCC AGTCGCCCTG GCGCATCTAC TTCCGGAAGG AGTTCTTCAC CCCCTGGCAT 
GACTCCCAGG AGGACCCCGT CAGTACCCAG CTCATCTACC GCCAAGTGCT GCATGGGGTC 
TGGTCTGGCG AGTACAACTT CGAGAAGGAG GATGATCTGG TACAGCTGCT GGCCAAGCAC 
TGCTACGTGC AGCTCGGTGC CTCGGTGGGG AGTGAAGCTG TCCAGGAGCT GCTGCCCAGC 
TGCATCCCCT CCAAGCTGTA CAAGACCAAG CCTCCAGAGA AGTGGGCTAG CCTCGTCACT 
GCTGCCCACG CCAAGGCACA GTACACCCAG AAGCAGGCAA AGCCGCTGGC TGTGCAGGAG 
CAGGTGGTGG ACACGGCCCG CCTGCAGTGG CCGCTGCTCT TCTCCCGGCT CTTTGAGGTC 
ACCACGCTCT CCGGCCCCCG ACTGCCCAAG ACTCAGCTGA TCTTAGCTGT TAACTGGAAG 
GGGATGTACT TCCTGGACCA GAGAGAGAAG ATACTCCTAG AACTCTCTTT TCCAGAGGTC 
GTGAGCCTGA TCACCAACAG AGAGGCGCAG GGTGGGCAGA GGCTGGTACT CTCCACGCTG 
CACGAAGAGG AGTACAAGTT CATGTCCCCC AGCAGCGTGG CCATCGCCGA GCTGGTAGCT 
ATGTTTCTGG AAGGCTTGAA GGAGAGGTCC GTGTTCGCCA TGGCTCTGCA GAACAGGAAG 
GCCACAGATG ACACCACCAT CCTGCCCTTC AAGAAGGGGG ACCTGCTGAT CCTGACAAAG 
AAGCAGGGGT TGTTGGCCTC TGAGAACTGG ACCTTGGGCC AGAACGACAG GACAGGCAAG 
ACGGGGCTGG TGCCCACAGC TTGCCTCTAC ACCATCCCAA CGGTCACCAA GCCCTCGGCA 
CAGCTGATGA GTTTGCTGGC CATGTCACCA GAGAAGCGGA AGCTGGCAGC CCAGGAGGTG 
CAGCCCACAG AGCCACTGCC TGAGGAGCAG CCCAAGGAGA AGCCACACAC CCTGGAGGAA 
TTCTCCTACG AGTTCTTTAG GGCCCCAGAG AAGGAGACAG TCAGGAGGAC CATGTTGCCC 
CTGGCCCGGA CACAGGGCCA CCTGTGGGCC CACTCATCCG AGCCACTGCG GCAGCCTCTG 
CTCAAGCAAG TCCACGCCAA TGCCGAGCTC CGGGACCCTG CCTGTCAGAT CTTCATTGGC 
ATCCTCCGGT ACATGGGTGA CTACCCATCT CGACAGGCTT GGACCTCCGT GGAGCTCGCC 
GACCAGATCT TCTCGTTGGC CCTCCAGGAG TCAGCCCTGC GGGATGAGGT CTACTGCCAG 
ATCCTGAAGC AGCTGACACA CAACACCACG AGGTACAGTG AGGAGCGGGG CTGGCAGCTG 
CTGTGGCTCT GCACTGGCCT CTTCCCCCCG GGAAAGGCGC TGCTGCCTCA TGCTCAGAAG 
TTCGTAGACA CGCGGAGGAA GAAGCTATTG GCTCCCGACT GCAGCCGGCG CATCCAGAGG 
ATCCTGAGGA CAGGACCCCG GAAGCAGCCC CCACACCCAG TGGAGGTGGA GGCCATAGAG 
CAGAACATCT CTCGCATCTG CCACAAGATC TACTTCCCTA ACGACACTAG CGAGATGCTG 
GAGGTGGGCA CCTACACTCG GGTGCGAGAT GTATGCCAGG GCATCGCCAC CAGGCTGCAG 
CTGTCTTCCT GGGAAGGCTG CAGCCTCTTC ATCAAGATTG CTGACAAGGT CATCAGCCAG 
AAGGAGGGAG ACTTCTTCTT TGACTCCTTG CGGCAAGTGT CTGATTGGGT GAAGAAAAAC 
AAGCCCCAGA AAGAAGGGGC GCCCGTGACA CTCCCCTACC AGGTGTACTT CATGCGGAAA 
TTGTGGCTCA ACGTGACCCC GGGGAAGGAT GTGAATGCAG ACACCGTACT TCATTACCAT 
CAGGAGCTGC CCAAGTACCT TCGTGGATTC CACAAGTGTT CACAGGAGGA TGCTGTCCAC 
CTGGCCGGCC TCATCTACAA GGCCCAGTTT GACAACAACC GGTCCCAGCT AGCTAGTATC 
CCCAAGATCC TGAGGGAGCT TGTGCCTGAG AACCTGACAC GTCTCATGTC CTCCGAGGAA 
TGGAGAAAGA ACATCCTTCT GGCCTATGAC AAGCACAAGG ACAAGACAGT AGAGGAGGCC 
AAGGTGGCGT TCCTCAAGGG GATCTGCCGG TGGCCCACAT TTGGGTCTGC CTTCTTCGAG 
GTGAAGCAAA CCTCGGAGCC TTCCTACCCA GACATCATCC TCATCGCCAT CAACCGGCAC 
GGGGTTCTGC TTATCCACCC CAAGACCAAG GAGCTGCTCA CCACCTACCC CTTCACCAAG 
ATCTCCAGCT GGAGCAGCGG CAGCACCTAC TTCCACATGG CACTGGGGAG CCTGGCCCGG 
GGCAGCCGCC TGCTGTGTGA AACCTCTCTG GGCTACAAGA TGGATGACCT GCTGACCTCG 
TATGTGCAGC AGCTCCTGAG CGCCATGAAC AAGCAGCGGG GCTCCCAGAC CCCGGCCCTG 
GCTGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_014121384.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976859.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.

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CloneID	OCe41358
Clone ID Related Accession (Same CDS sequence)	XM_014121384.1
Accession Version	XM_014121384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	unconventional myosin-VIIb isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG TAGCAGCAGG GCCCCGGGGG 
GGGCGGGTCC AATTACCCTG CACAGGTGGT ATCCCCTGCC CTCCTGTCCC CCCTCACATG 
GCTCCCCGGG CTCTGGAGGA CCCCAGGACA AGCTCCTTCC TAGGGCGCCT AGGACCCCTG 
GGCTTCAGCT GTGTCCTACT CCCCCTGGCC CACTCTGTCC CCAGGAGCTG CTCACCACCT 
ACCCCTTCAC CAAGATCTCC AGCTGGAGCA GCGGCAGCAC CTACTTCCAC ATGGCACTGG 
GGAGCCTGGC CCGGGGCAGC CGCCTGCTGT GTGAAACCTC TCTGGGCTAC AAGATGGATG 
ACCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976859.1
CDS	154..6762
Translation	MVQPPLRELLGARIQAGRTCCLRMSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKVAAGPRGGRVQLPCTGGIPCPPVPPHMAPRALEDPRTSSFLGRLGPLGFSCVLLPLAHSVPRSCSPPTPSPRSPAGAAAAPTSTWHWGAWPGAAACCVKPLWATRWMTC

Target ORF information:

RefSeq Version	XM_014121384.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121384.1

ORF Insert Sequence:

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG TAGCAGCAGG GCCCCGGGGG 
GGGCGGGTCC AATTACCCTG CACAGGTGGT ATCCCCTGCC CTCCTGTCCC CCCTCACATG 
GCTCCCCGGG CTCTGGAGGA CCCCAGGACA AGCTCCTTCC TAGGGCGCCT AGGACCCCTG 
GGCTTCAGCT GTGTCCTACT CCCCCTGGCC CACTCTGTCC CCAGGAGCTG CTCACCACCT 
ACCCCTTCAC CAAGATCTCC AGCTGGAGCA GCGGCAGCAC CTACTTCCAC ATGGCACTGG 
GGAGCCTGGC CCGGGGCAGC CGCCTGCTGT GTGAAACCTC TCTGGGCTAC AAGATGGATG 
ACCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_014121385.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976860.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.

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CloneID	OCe41359
Clone ID Related Accession (Same CDS sequence)	XM_014121385.1
Accession Version	XM_014121385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	unconventional myosin-VIIb isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG TGAGTCCCAG GTCCCTTCCC 
CTCATCCAGA CCTACAGGAC GCGGAGGCCC CAGGGACTAC GTTTCCCCAA CCCTGACCCC 
AGACTGCCAC CAGGCAAGTC GCTCCCTGTG AGGGCCTGGC TTCCTGTGCC CAGGGTGAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976860.1
CDS	154..6696
Translation	MVQPPLRELLGARIQAGRTCCLRMSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKELLTTYPFTKISSWSSGSTYFHMALGSLARGSRLLCETSLVSPRSLPLIQTYRTRRPQGLRFPNPDPRLPPGKSLPVRAWLPVPRVS

Target ORF information:

RefSeq Version	XM_014121385.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121385.1

ORF Insert Sequence:

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG TGAGTCCCAG GTCCCTTCCC 
CTCATCCAGA CCTACAGGAC GCGGAGGCCC CAGGGACTAC GTTTCCCCAA CCCTGACCCC 
AGACTGCCAC CAGGCAAGTC GCTCCCTGTG AGGGCCTGGC TTCCTGTGCC CAGGGTGAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_014121386.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976861.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.

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CloneID	OCe41360
Clone ID Related Accession (Same CDS sequence)	XM_014121386.1
Accession Version	XM_014121386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	unconventional myosin-VIIb isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCT TCAGGCTGGG TGATCATGTG TGGCTAAACC CCCTCTCTGC CAACAAGACC 
AGTGTGGCCA TTGGATCCAT TGTCAAGGAA ACAAAGCCTG GCAAGATCTT GGTTGTAGAT 
GATGAAGGCA AGGAACACTG GATCCAAGCA AAAGACCTTG ACACCCTCAG CCCCATGCAC 
CCCAACTCAG TGCAAGGCGT GGATGACATG ATCCGCCTGG GGGAACTGAA CGAGGCAGGC 
ATGGTGCACA ACCTCCTGAT CCGCTACCGG CAGCACAAGA TCTATACATA CACAGGCTCC 
ATTCTGATGG CCGTGAACCC CTTCCAAGTG TTGCCCCTCT ACACCCTGGA GCAGGTACAG 
CTGTACTACA ACCGTCACAT GGGCGAACTA CCACCCCATG TCTTTGCCAT CGCCAACAAC 
TGCTACTTCA ACATGAAGAG GAACAAGAGG GACCAGTGCT GCATTATCAG TGGTGAGTCT 
GGGGCAGGGA AGACAGAGAC CACGAAGCTC ATCCTGCAGT TCCTGGCCAC CGTCAGTGGC 
CAACATTCCT GGATTGAGCA GCAGGTCTTG GAGGCCAACC CCATCCTGGA AGCCTTCGGA 
AATGCCAAGA CAGTCAGAAA TGATAACTCA AGCCGCTTTG GGAAATATAT CGACATCCAC 
TTCAACCCCA ATGGGGTGAT TGAGGGCGCA CGCATCGAGC AATTTCTCCT GGAGAAGTCC 
CGGGTCTGCC GGCAGGCTCC CGAGGAGCGG AACTACCACA TCTTCTACGG CATGCTCCTG 
GGGATGACCG CGGAGGAGAA GAAGCTGCTG AGCCTGGGGA CGCCCTCCGA GTACCACTAC 
CTGACCACGG GAAACTGCAC ATCCTGTGAG GGCCTCAATG ATGCCAAGGA CTATGCCCAT 
GTTCGCTCTG CCATGAAGAT CCTCATGTTC TCCGACTCTG AGAACTGGGA CCTCAGCAAG 
CTGCTGGCAG CCATTCTCCA CCTGGGCAAT GTGGAGTTTA TGGCTGCAGT CTTCGAGAAC 
CTGGATTCCT CCGACGTGAT GGAGACGCCT GCCTTTCCCA CCGTGCTGAA GTTACTGGAG 
GTGGAGTACC AGGCACTCCG GGACTGTCTG ATCAAGCATT CCATCATCAT CCGAGGGGAG 
TTTGTCACCA GGCCCCTGAA CATCACTCAG GCTGCAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCACCTCTT CCTGTGGATT GTCAAGAAGA TCAACACTGC AATTTTCACT 
CCACCTGCCC AGGACCCCAA AAATGTGCGA AGAGCCATTG GCCTCCTGGA CATATTTGGC 
TTTGAAAATT TCCAGAACAA TAGCTTCGAG CAGCTCTGCA TCAACTTTGC CAACGAGCAC 
CTGCAGCAGT TCTTCGTGCA GCATGTGTTC ACCATGGAGC AGGAGGAGTA CCTCTCAGAG 
AACATTGCCT GGGACTACAT CCACTACAAT GACAATCGGC CCACCCTGGA TCTGCTGGCC 
CTTAAGCCCA TGAGCATCAT CTCCCTCCTG GACGAGGAGA GCCACTTCCC ACAGGGGACA 
GATACAACCA TGCTGCAGAA ACTGAACAGT GTCCATGCCA ACAATAAGGC CTACCTACAG 
CCCAGGAACA TCCATGATGC CAGGTTTGGC ATCGCCCACT TTGCAGGCGA GGTGTACTAC 
CAGACAGAAG GCTTCCTGGA GAAGAATCGA GACATGCTGA GCACAGATAT CCTCACTCTG 
GTCTACTCCT CCAAAAACAA GTTCCTGAAG GAGATATTCA AGCTGGAGTC AGCGGGGACC 
AAGCTGGGCC AAAGCACCAT CATCCAGGCT AGCGCAGGGA GCCAGCTCTT CAAGTCTGCA 
GACTCCAGCA AGCAGCCCTC CACATTGGCA GGCCAGTTCA AGAAGTCCCT GGACCAGCTG 
ATGAAAATCC TGACCAGCTG CCAGCCCTAC TTTATCCGCT GCATCAAACC CAATGAGTAC 
AAGAAGCCAC TGCTCTTCGA CCGCGACCTG TGCCTCCGGC AGCTGCGTTA CTCGGGGATG 
ATGGAGACCG TGCACATCCG CAGGTCCGGC TTCCCCATCC GGTACACGTT CCAGGAGTTC 
GCGCAGAGGT TCGGCGTGCT GCTGCCCAGC GCCGTTCGCC TGCAGCTTCG AGACAAGGCT 
CGCCAGATGA CCCTGCGCAT TGCTGAAATG CAGCTGGGGA CGGACAAAGA ATGGAAAGTG 
GGAAAGACCA AAATTTTCCT GAAGGATAAC CAGGACACTC TACTTGAGGT ACAGAGGACC 
CAGACCCTGG ACAAGGCAGC AATCAACATC CAAAGAGTAC TGCGAGGCTA CAAATACAGG 
AAGGAGTTCT TGAGACAGAG GCGGGCCGCT GTGACCCTCC AGGCCAGGTG GAGAGGCTAC 
TACAACAAGC GGAACTTCAA GCAGATCCTC TTGGGTTTTG AGCGCCTGCA GGCCATTGCC 
CGGAGCCAGT GGCTAGCAAA GCAGTACCAG ACCATGCGGC AGAGGATGGT CCAGCTGCAG 
GCACTCTGCA GGGGCTACCT TGTGCGCCAG CAAGTCCAGG CCAAGAGAAG GGCAGTGGTG 
GTCATACAGG CACATGCCAG GGGCATGGCT GCGCGGCGGA ACTTCCAGCA GCAGAAAGCC 
AATGGGCCTC TGGTCATCCC AGCTGAAGGG CAGAAGAGCC AAAGTATTCT CCCCACCAGG 
AAGCGTAAGT CAATCTACGA TACCATCACT GACACGGAGA TGGTGGAGCA AGTGTTTGGC 
TTCCTCCCTT CCATGATTGG TGGGCAGGAG GGCCAGGCTC CATGGCGCTT TGAGGACCTG 
GAAGCGAAGA GCCAGAAGCT GCCCGAGGTT GACCTGGACA CGGTCCCCCT GGTGGAGGAG 
TCTGAGGACG ATGTGGACGG GCTGGCCGAG TACACCTTCC CCAAGTTTGC TGCCACCTAC 
TTCCAGAAAT CGGCCAGCCA CACGCACATC CGGCGGCCCC TCCGATACCC ACTGCTCTAC 
CATGAGGAAG ATGCTGACTG CTCGGCCGCC CTGGCCGTGT GGAACATCAT TCTGAGATTC 
ATGGGTGACC TTCCAGAGCC AGTGCTTTTT GCCAGGAATG GCCCGCCCGG CGGCTCCGTG 
ACGCGGCAGA TCCGTGACAG CCTGGGCAGG GAGGGCGGCA CCCGGTCCCC ACCGCACTGC 
GGGTCCACAC AGAAGTCCAG CCGCAGAGAC AGAGGGAGCA AGGACATCTC CTCCATGAAG 
CTAAAGCGGT CCTCCAAGAT CACAAGCCAG GTAACCAGCC AGCTGAATAT TGGAGAGGAG 
GCATTTGAGC CTGATGGCCC CATCTCAGAC CGACCCATGT CCAATCTGGA GAAGGTACAC 
TTCATTGTGG GCTATGCTAT CCTGCGGCCT AACCTCAGGG ACGAGATTTA CTGCCAGATC 
TGCAAGCAGC TGTCCGAGAA CTTCAAAACA AGCTGCCTGG CCCGGGGCTG GATCCTGCTC 
AGCCTTTGCT TGGGCTGTTT CCCACCCTCC GAGAGGTTCA TGAAGTATCT GCTAAACTTC 
ATTGGTCAAG GGCCAGCTGG CTATGGGCCC TTCTGTGCAG AACGCCTGAG ACGAACCTAT 
GCTAATGGGG TACGCACGGA GCCCCCCACG TGGCTGGAGC TGCAGGCAGT CAAATCCAAG 
AAGCACATCC CAATCCATGT CATCTTGGTG ACCGGAGAGA GCCTGACTGT CATGGTTGAC 
TCTGCCTCGA CATCTCGGGA AGTCTGCCTG CACATTGCCC ACAAGCAGGG CCTCAGCGAT 
CACCTGGGCT TTTCTCTCCA GGTGGCCGTG TACGACAAGT TCTGGTCCCT GGGCAGCGGG 
CGCGACCACG TGATGGATAC AGTCGCCCAG TGTGAGCAGC TGGCCCGGGA CAGGGGGGAG 
AACGAGCGCC AGTCGCCCTG GCGCATCTAC TTCCGGAAGG AGTTCTTCAC CCCCTGGCAT 
GACTCCCAGG AGGACCCCGT CAGTACCCAG CTCATCTACC GCCAAGTGCT GCATGGGGTC 
TGGTCTGGCG AGTACAACTT CGAGAAGGAG GATGATCTGG TACAGCTGCT GGCCAAGCAC 
TGCTACGTGC AGCTCGGTGC CTCGGTGGGG AGTGAAGCTG TCCAGGAGCT GCTGCCCAGC 
TGCATCCCCT CCAAGCTGTA CAAGACCAAG CCTCCAGAGA AGTGGGCTAG CCTCGTCACT 
GCTGCCCACG CCAAGGCACA GTACACCCAG AAGCAGGCAA AGCCGCTGGC TGTGCAGGAG 
CAGGTGGTGG ACACGGCCCG CCTGCAGTGG CCGCTGCTCT TCTCCCGGCT CTTTGAGGTC 
ACCACGCTCT CCGGCCCCCG ACTGCCCAAG ACTCAGCTGA TCTTAGCTGT TAACTGGAAG 
GGGATGTACT TCCTGGACCA GAGAGAGAAG ATACTCCTAG AACTCTCTTT TCCAGAGGTC 
GTGAGCCTGA TCACCAACAG AGAGGCGCAG GGTGGGCAGA GGCTGGTACT CTCCACGCTG 
CACGAAGAGG AGTACAAGTT CATGTCCCCC AGCAGCGTGG CCATCGCCGA GCTGGTAGCT 
ATGTTTCTGG AAGGCTTGAA GGAGAGGTCC GTGTTCGCCA TGGCTCTGCA GAACAGGAAG 
GCCACAGATG ACACCACCAT CCTGCCCTTC AAGAAGGGGG ACCTGCTGAT CCTGACAAAG 
AAGCAGGGGT TGTTGGCCTC TGAGAACTGG ACCTTGGGCC AGAACGACAG GACAGGCAAG 
ACGGGGCTGG TGCCCACAGC TTGCCTCTAC ACCATCCCAA CGGTCACCAA GCCCTCGGCA 
CAGCTGATGA GTTTGCTGGC CATGTCACCA GAGAAGCGGA AGCTGGCAGC CCAGGAGGTG 
CAGCCCACAG AGCCACTGCC TGAGGAGCAG CCCAAGGAGA AGCCACACAC CCTGGAGGAA 
TTCTCCTACG AGTTCTTTAG GGCCCCAGAG AAGGAGACAG TCAGGAGGAC CATGTTGCCC 
CTGGCCCGGA CACAGGGCCA CCTGTGGGCC CACTCATCCG AGCCACTGCG GCAGCCTCTG 
CTCAAGCAAG TCCACGCCAA TGCCGAGCTC CGGGACCCTG CCTGTCAGAT CTTCATTGGC 
ATCCTCCGGT ACATGGGTGA CTACCCATCT CGACAGGCTT GGACCTCCGT GGAGCTCGCC 
GACCAGATCT TCTCGTTGGC CCTCCAGGAG TCAGCCCTGC GGGATGAGGT CTACTGCCAG 
ATCCTGAAGC AGCTGACACA CAACACCACG AGGTACAGTG AGGAGCGGGG CTGGCAGCTG 
CTGTGGCTCT GCACTGGCCT CTTCCCCCCG GGAAAGGCGC TGCTGCCTCA TGCTCAGAAG 
TTCGTAGACA CGCGGAGGAA GAAGCTATTG GCTCCCGACT GCAGCCGGCG CATCCAGAGG 
ATCCTGAGGA CAGGACCCCG GAAGCAGCCC CCACACCCAG TGGAGGTGGA GGCCATAGAG 
CAGAACATCT CTCGCATCTG CCACAAGATC TACTTCCCTA ACGACACTAG CGAGATGCTG 
GAGGTGGGCA CCTACACTCG GGTGCGAGAT GTATGCCAGG GCATCGCCAC CAGGCTGCAG 
CTGTCTTCCT GGGAAGGCTG CAGCCTCTTC ATCAAGATTG CTGACAAGGT CATCAGCCAG 
AAGGAGGGAG ACTTCTTCTT TGACTCCTTG CGGCAAGTGT CTGATTGGGT GAAGAAAAAC 
AAGCCCCAGA AAGAAGGGGC GCCCGTGACA CTCCCCTACC AGGTGTACTT CATGCGGAAA 
TTGTGGCTCA ACGTGACCCC GGGGAAGGAT GTGAATGCAG ACACCGTACT TCATTACCAT 
CAGGAGCTGC CCAAGTACCT TCGTGGATTC CACAAGTGTT CACAGGAGGA TGCTGTCCAC 
CTGGCCGGCC TCATCTACAA GGCCCAGTTT GACAACAACC GGTCCCAGCT AGCTAGTATC 
CCCAAGATCC TGAGGGAGCT TGTGCCTGAG AACCTGACAC GTCTCATGTC CTCCGAGGAA 
TGGAGAAAGA ACATCCTTCT GGCCTATGAC AAGCACAAGG ACAAGACAGT AGAGGAGGCC 
AAGGTGGCGT TCCTCAAGGG GATCTGCCGG TGGCCCACAT TTGGGTCTGC CTTCTTCGAG 
GTGAAGCAAA CCTCGGAGCC TTCCTACCCA GACATCATCC TCATCGCCAT CAACCGGCAC 
GGGGTTCTGC TTATCCACCC CAAGACCAAG GTAGCAGCAG GGCCCCGGGG GGGGCGGGTC 
CAATTACCCT GCACAGGTGG TATCCCCTGC CCTCCTGTCC CCCCTCACAT GGCTCCCCGG 
GCTCTGGAGG ACCCCAGGAC AAGCTCCTTC CTAGGGCGCC TAGGACCCCT GGGCTTCAGC 
TGTGTCCTAC TCCCCCTGGC CCACTCTGTC CCCAGGAGCT GCTCACCACC TACCCCTTCA 
CCAAGATCTC CAGCTGGAGC AGCGGCAGCA CCTACTTCCA CATGGCACTG GGGAGCCTGG 
CCCGGGGCAG CCGCCTGCTG TGTGAAACCT CTCTGGGCTA CAAGATGGAT GACCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976861.1
CDS	1011..7550
Translation	MSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKVAAGPRGGRVQLPCTGGIPCPPVPPHMAPRALEDPRTSSFLGRLGPLGFSCVLLPLAHSVPRSCSPPTPSPRSPAGAAAAPTSTWHWGAWPGAAACCVKPLWATRWMTC

Target ORF information:

RefSeq Version	XM_014121386.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121386.1

ORF Insert Sequence:

ATGTCAGCCT TCAGGCTGGG TGATCATGTG TGGCTAAACC CCCTCTCTGC CAACAAGACC 
AGTGTGGCCA TTGGATCCAT TGTCAAGGAA ACAAAGCCTG GCAAGATCTT GGTTGTAGAT 
GATGAAGGCA AGGAACACTG GATCCAAGCA AAAGACCTTG ACACCCTCAG CCCCATGCAC 
CCCAACTCAG TGCAAGGCGT GGATGACATG ATCCGCCTGG GGGAACTGAA CGAGGCAGGC 
ATGGTGCACA ACCTCCTGAT CCGCTACCGG CAGCACAAGA TCTATACATA CACAGGCTCC 
ATTCTGATGG CCGTGAACCC CTTCCAAGTG TTGCCCCTCT ACACCCTGGA GCAGGTACAG 
CTGTACTACA ACCGTCACAT GGGCGAACTA CCACCCCATG TCTTTGCCAT CGCCAACAAC 
TGCTACTTCA ACATGAAGAG GAACAAGAGG GACCAGTGCT GCATTATCAG TGGTGAGTCT 
GGGGCAGGGA AGACAGAGAC CACGAAGCTC ATCCTGCAGT TCCTGGCCAC CGTCAGTGGC 
CAACATTCCT GGATTGAGCA GCAGGTCTTG GAGGCCAACC CCATCCTGGA AGCCTTCGGA 
AATGCCAAGA CAGTCAGAAA TGATAACTCA AGCCGCTTTG GGAAATATAT CGACATCCAC 
TTCAACCCCA ATGGGGTGAT TGAGGGCGCA CGCATCGAGC AATTTCTCCT GGAGAAGTCC 
CGGGTCTGCC GGCAGGCTCC CGAGGAGCGG AACTACCACA TCTTCTACGG CATGCTCCTG 
GGGATGACCG CGGAGGAGAA GAAGCTGCTG AGCCTGGGGA CGCCCTCCGA GTACCACTAC 
CTGACCACGG GAAACTGCAC ATCCTGTGAG GGCCTCAATG ATGCCAAGGA CTATGCCCAT 
GTTCGCTCTG CCATGAAGAT CCTCATGTTC TCCGACTCTG AGAACTGGGA CCTCAGCAAG 
CTGCTGGCAG CCATTCTCCA CCTGGGCAAT GTGGAGTTTA TGGCTGCAGT CTTCGAGAAC 
CTGGATTCCT CCGACGTGAT GGAGACGCCT GCCTTTCCCA CCGTGCTGAA GTTACTGGAG 
GTGGAGTACC AGGCACTCCG GGACTGTCTG ATCAAGCATT CCATCATCAT CCGAGGGGAG 
TTTGTCACCA GGCCCCTGAA CATCACTCAG GCTGCAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCACCTCTT CCTGTGGATT GTCAAGAAGA TCAACACTGC AATTTTCACT 
CCACCTGCCC AGGACCCCAA AAATGTGCGA AGAGCCATTG GCCTCCTGGA CATATTTGGC 
TTTGAAAATT TCCAGAACAA TAGCTTCGAG CAGCTCTGCA TCAACTTTGC CAACGAGCAC 
CTGCAGCAGT TCTTCGTGCA GCATGTGTTC ACCATGGAGC AGGAGGAGTA CCTCTCAGAG 
AACATTGCCT GGGACTACAT CCACTACAAT GACAATCGGC CCACCCTGGA TCTGCTGGCC 
CTTAAGCCCA TGAGCATCAT CTCCCTCCTG GACGAGGAGA GCCACTTCCC ACAGGGGACA 
GATACAACCA TGCTGCAGAA ACTGAACAGT GTCCATGCCA ACAATAAGGC CTACCTACAG 
CCCAGGAACA TCCATGATGC CAGGTTTGGC ATCGCCCACT TTGCAGGCGA GGTGTACTAC 
CAGACAGAAG GCTTCCTGGA GAAGAATCGA GACATGCTGA GCACAGATAT CCTCACTCTG 
GTCTACTCCT CCAAAAACAA GTTCCTGAAG GAGATATTCA AGCTGGAGTC AGCGGGGACC 
AAGCTGGGCC AAAGCACCAT CATCCAGGCT AGCGCAGGGA GCCAGCTCTT CAAGTCTGCA 
GACTCCAGCA AGCAGCCCTC CACATTGGCA GGCCAGTTCA AGAAGTCCCT GGACCAGCTG 
ATGAAAATCC TGACCAGCTG CCAGCCCTAC TTTATCCGCT GCATCAAACC CAATGAGTAC 
AAGAAGCCAC TGCTCTTCGA CCGCGACCTG TGCCTCCGGC AGCTGCGTTA CTCGGGGATG 
ATGGAGACCG TGCACATCCG CAGGTCCGGC TTCCCCATCC GGTACACGTT CCAGGAGTTC 
GCGCAGAGGT TCGGCGTGCT GCTGCCCAGC GCCGTTCGCC TGCAGCTTCG AGACAAGGCT 
CGCCAGATGA CCCTGCGCAT TGCTGAAATG CAGCTGGGGA CGGACAAAGA ATGGAAAGTG 
GGAAAGACCA AAATTTTCCT GAAGGATAAC CAGGACACTC TACTTGAGGT ACAGAGGACC 
CAGACCCTGG ACAAGGCAGC AATCAACATC CAAAGAGTAC TGCGAGGCTA CAAATACAGG 
AAGGAGTTCT TGAGACAGAG GCGGGCCGCT GTGACCCTCC AGGCCAGGTG GAGAGGCTAC 
TACAACAAGC GGAACTTCAA GCAGATCCTC TTGGGTTTTG AGCGCCTGCA GGCCATTGCC 
CGGAGCCAGT GGCTAGCAAA GCAGTACCAG ACCATGCGGC AGAGGATGGT CCAGCTGCAG 
GCACTCTGCA GGGGCTACCT TGTGCGCCAG CAAGTCCAGG CCAAGAGAAG GGCAGTGGTG 
GTCATACAGG CACATGCCAG GGGCATGGCT GCGCGGCGGA ACTTCCAGCA GCAGAAAGCC 
AATGGGCCTC TGGTCATCCC AGCTGAAGGG CAGAAGAGCC AAAGTATTCT CCCCACCAGG 
AAGCGTAAGT CAATCTACGA TACCATCACT GACACGGAGA TGGTGGAGCA AGTGTTTGGC 
TTCCTCCCTT CCATGATTGG TGGGCAGGAG GGCCAGGCTC CATGGCGCTT TGAGGACCTG 
GAAGCGAAGA GCCAGAAGCT GCCCGAGGTT GACCTGGACA CGGTCCCCCT GGTGGAGGAG 
TCTGAGGACG ATGTGGACGG GCTGGCCGAG TACACCTTCC CCAAGTTTGC TGCCACCTAC 
TTCCAGAAAT CGGCCAGCCA CACGCACATC CGGCGGCCCC TCCGATACCC ACTGCTCTAC 
CATGAGGAAG ATGCTGACTG CTCGGCCGCC CTGGCCGTGT GGAACATCAT TCTGAGATTC 
ATGGGTGACC TTCCAGAGCC AGTGCTTTTT GCCAGGAATG GCCCGCCCGG CGGCTCCGTG 
ACGCGGCAGA TCCGTGACAG CCTGGGCAGG GAGGGCGGCA CCCGGTCCCC ACCGCACTGC 
GGGTCCACAC AGAAGTCCAG CCGCAGAGAC AGAGGGAGCA AGGACATCTC CTCCATGAAG 
CTAAAGCGGT CCTCCAAGAT CACAAGCCAG GTAACCAGCC AGCTGAATAT TGGAGAGGAG 
GCATTTGAGC CTGATGGCCC CATCTCAGAC CGACCCATGT CCAATCTGGA GAAGGTACAC 
TTCATTGTGG GCTATGCTAT CCTGCGGCCT AACCTCAGGG ACGAGATTTA CTGCCAGATC 
TGCAAGCAGC TGTCCGAGAA CTTCAAAACA AGCTGCCTGG CCCGGGGCTG GATCCTGCTC 
AGCCTTTGCT TGGGCTGTTT CCCACCCTCC GAGAGGTTCA TGAAGTATCT GCTAAACTTC 
ATTGGTCAAG GGCCAGCTGG CTATGGGCCC TTCTGTGCAG AACGCCTGAG ACGAACCTAT 
GCTAATGGGG TACGCACGGA GCCCCCCACG TGGCTGGAGC TGCAGGCAGT CAAATCCAAG 
AAGCACATCC CAATCCATGT CATCTTGGTG ACCGGAGAGA GCCTGACTGT CATGGTTGAC 
TCTGCCTCGA CATCTCGGGA AGTCTGCCTG CACATTGCCC ACAAGCAGGG CCTCAGCGAT 
CACCTGGGCT TTTCTCTCCA GGTGGCCGTG TACGACAAGT TCTGGTCCCT GGGCAGCGGG 
CGCGACCACG TGATGGATAC AGTCGCCCAG TGTGAGCAGC TGGCCCGGGA CAGGGGGGAG 
AACGAGCGCC AGTCGCCCTG GCGCATCTAC TTCCGGAAGG AGTTCTTCAC CCCCTGGCAT 
GACTCCCAGG AGGACCCCGT CAGTACCCAG CTCATCTACC GCCAAGTGCT GCATGGGGTC 
TGGTCTGGCG AGTACAACTT CGAGAAGGAG GATGATCTGG TACAGCTGCT GGCCAAGCAC 
TGCTACGTGC AGCTCGGTGC CTCGGTGGGG AGTGAAGCTG TCCAGGAGCT GCTGCCCAGC 
TGCATCCCCT CCAAGCTGTA CAAGACCAAG CCTCCAGAGA AGTGGGCTAG CCTCGTCACT 
GCTGCCCACG CCAAGGCACA GTACACCCAG AAGCAGGCAA AGCCGCTGGC TGTGCAGGAG 
CAGGTGGTGG ACACGGCCCG CCTGCAGTGG CCGCTGCTCT TCTCCCGGCT CTTTGAGGTC 
ACCACGCTCT CCGGCCCCCG ACTGCCCAAG ACTCAGCTGA TCTTAGCTGT TAACTGGAAG 
GGGATGTACT TCCTGGACCA GAGAGAGAAG ATACTCCTAG AACTCTCTTT TCCAGAGGTC 
GTGAGCCTGA TCACCAACAG AGAGGCGCAG GGTGGGCAGA GGCTGGTACT CTCCACGCTG 
CACGAAGAGG AGTACAAGTT CATGTCCCCC AGCAGCGTGG CCATCGCCGA GCTGGTAGCT 
ATGTTTCTGG AAGGCTTGAA GGAGAGGTCC GTGTTCGCCA TGGCTCTGCA GAACAGGAAG 
GCCACAGATG ACACCACCAT CCTGCCCTTC AAGAAGGGGG ACCTGCTGAT CCTGACAAAG 
AAGCAGGGGT TGTTGGCCTC TGAGAACTGG ACCTTGGGCC AGAACGACAG GACAGGCAAG 
ACGGGGCTGG TGCCCACAGC TTGCCTCTAC ACCATCCCAA CGGTCACCAA GCCCTCGGCA 
CAGCTGATGA GTTTGCTGGC CATGTCACCA GAGAAGCGGA AGCTGGCAGC CCAGGAGGTG 
CAGCCCACAG AGCCACTGCC TGAGGAGCAG CCCAAGGAGA AGCCACACAC CCTGGAGGAA 
TTCTCCTACG AGTTCTTTAG GGCCCCAGAG AAGGAGACAG TCAGGAGGAC CATGTTGCCC 
CTGGCCCGGA CACAGGGCCA CCTGTGGGCC CACTCATCCG AGCCACTGCG GCAGCCTCTG 
CTCAAGCAAG TCCACGCCAA TGCCGAGCTC CGGGACCCTG CCTGTCAGAT CTTCATTGGC 
ATCCTCCGGT ACATGGGTGA CTACCCATCT CGACAGGCTT GGACCTCCGT GGAGCTCGCC 
GACCAGATCT TCTCGTTGGC CCTCCAGGAG TCAGCCCTGC GGGATGAGGT CTACTGCCAG 
ATCCTGAAGC AGCTGACACA CAACACCACG AGGTACAGTG AGGAGCGGGG CTGGCAGCTG 
CTGTGGCTCT GCACTGGCCT CTTCCCCCCG GGAAAGGCGC TGCTGCCTCA TGCTCAGAAG 
TTCGTAGACA CGCGGAGGAA GAAGCTATTG GCTCCCGACT GCAGCCGGCG CATCCAGAGG 
ATCCTGAGGA CAGGACCCCG GAAGCAGCCC CCACACCCAG TGGAGGTGGA GGCCATAGAG 
CAGAACATCT CTCGCATCTG CCACAAGATC TACTTCCCTA ACGACACTAG CGAGATGCTG 
GAGGTGGGCA CCTACACTCG GGTGCGAGAT GTATGCCAGG GCATCGCCAC CAGGCTGCAG 
CTGTCTTCCT GGGAAGGCTG CAGCCTCTTC ATCAAGATTG CTGACAAGGT CATCAGCCAG 
AAGGAGGGAG ACTTCTTCTT TGACTCCTTG CGGCAAGTGT CTGATTGGGT GAAGAAAAAC 
AAGCCCCAGA AAGAAGGGGC GCCCGTGACA CTCCCCTACC AGGTGTACTT CATGCGGAAA 
TTGTGGCTCA ACGTGACCCC GGGGAAGGAT GTGAATGCAG ACACCGTACT TCATTACCAT 
CAGGAGCTGC CCAAGTACCT TCGTGGATTC CACAAGTGTT CACAGGAGGA TGCTGTCCAC 
CTGGCCGGCC TCATCTACAA GGCCCAGTTT GACAACAACC GGTCCCAGCT AGCTAGTATC 
CCCAAGATCC TGAGGGAGCT TGTGCCTGAG AACCTGACAC GTCTCATGTC CTCCGAGGAA 
TGGAGAAAGA ACATCCTTCT GGCCTATGAC AAGCACAAGG ACAAGACAGT AGAGGAGGCC 
AAGGTGGCGT TCCTCAAGGG GATCTGCCGG TGGCCCACAT TTGGGTCTGC CTTCTTCGAG 
GTGAAGCAAA CCTCGGAGCC TTCCTACCCA GACATCATCC TCATCGCCAT CAACCGGCAC 
GGGGTTCTGC TTATCCACCC CAAGACCAAG GTAGCAGCAG GGCCCCGGGG GGGGCGGGTC 
CAATTACCCT GCACAGGTGG TATCCCCTGC CCTCCTGTCC CCCCTCACAT GGCTCCCCGG 
GCTCTGGAGG ACCCCAGGAC AAGCTCCTTC CTAGGGCGCC TAGGACCCCT GGGCTTCAGC 
TGTGTCCTAC TCCCCCTGGC CCACTCTGTC CCCAGGAGCT GCTCACCACC TACCCCTTCA 
CCAAGATCTC CAGCTGGAGC AGCGGCAGCA CCTACTTCCA CATGGCACTG GGGAGCCTGG 
CCCGGGGCAG CCGCCTGCTG TGTGAAACCT CTCTGGGCTA CAAGATGGAT GACCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_014121387.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976862.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.

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CloneID	OCe41361
Clone ID Related Accession (Same CDS sequence)	XM_014121387.2 , XM_014121387.1
Accession Version	XM_014121387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	unconventional myosin-VIIb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG GCTACAAGAT GGATGACCTG 
CTGACCTCGT ATGTGCAGCA GCTCCTGAGC GCCATGAACA AGCAGCGGGG CTCCCAGACC 
CCGGCCCTGG CTGATCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976862.1
CDS	311..6811
Translation	MVQPPLRELLGARIQAGRTCCLRMSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKELLTTYPFTKISSWSSGSTYFHMALGSLARGSRLLCETSLGYKMDDLLTSYVQQLLSAMNKQRGSQTPALADP

Target ORF information:

RefSeq Version	XM_014121387.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121387.2

ORF Insert Sequence:

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG GCTACAAGAT GGATGACCTG 
CTGACCTCGT ATGTGCAGCA GCTCCTGAGC GCCATGAACA AGCAGCGGGG CTCCCAGACC 
CCGGCCCTGG CTGATCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7B ( XM_014121387.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976862.1 Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X4, mRNA.

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CloneID	OCe41361
Clone ID Related Accession (Same CDS sequence)	XM_014121387.2 , XM_014121387.1
Accession Version	XM_014121387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	unconventional myosin-VIIb isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG GCTACAAGAT GGATGACCTG 
CTGACCTCGT ATGTGCAGCA GCTCCTGAGC GCCATGAACA AGCAGCGGGG CTCCCAGACC 
CCGGCCCTGG CTGATCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976862.1
CDS	152..6652
Translation	MVQPPLRELLGARIQAGRTCCLRMSAFRLGDHVWLNPLSANKTSVAIGSIVKETKPGKILVVDDEGKEHWIQAKDLDTLSPMHPNSVQGVDDMIRLGELNEAGMVHNLLIRYRQHKIYTYTGSILMAVNPFQVLPLYTLEQVQLYYNRHMGELPPHVFAIANNCYFNMKRNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTVRNDNSSRFGKYIDIHFNPNGVIEGARIEQFLLEKSRVCRQAPEERNYHIFYGMLLGMTAEEKKLLSLGTPSEYHYLTTGNCTSCEGLNDAKDYAHVRSAMKILMFSDSENWDLSKLLAAILHLGNVEFMAAVFENLDSSDVMETPAFPTVLKLLEVEYQALRDCLIKHSIIIRGEFVTRPLNITQAADRRDAFVKGIYGHLFLWIVKKINTAIFTPPAQDPKNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVQHVFTMEQEEYLSENIAWDYIHYNDNRPTLDLLALKPMSIISLLDEESHFPQGTDTTMLQKLNSVHANNKAYLQPRNIHDARFGIAHFAGEVYYQTEGFLEKNRDMLSTDILTLVYSSKNKFLKEIFKLESAGTKLGQSTIIQASAGSQLFKSADSSKQPSTLAGQFKKSLDQLMKILTSCQPYFIRCIKPNEYKKPLLFDRDLCLRQLRYSGMMETVHIRRSGFPIRYTFQEFAQRFGVLLPSAVRLQLRDKARQMTLRIAEMQLGTDKEWKVGKTKIFLKDNQDTLLEVQRTQTLDKAAINIQRVLRGYKYRKEFLRQRRAAVTLQARWRGYYNKRNFKQILLGFERLQAIARSQWLAKQYQTMRQRMVQLQALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQQKANGPLVIPAEGQKSQSILPTRKRKSIYDTITDTEMVEQVFGFLPSMIGGQEGQAPWRFEDLEAKSQKLPEVDLDTVPLVEESEDDVDGLAEYTFPKFAATYFQKSASHTHIRRPLRYPLLYHEEDADCSAALAVWNIILRFMGDLPEPVLFARNGPPGGSVTRQIRDSLGREGGTRSPPHCGSTQKSSRRDRGSKDISSMKLKRSSKITSQVTSQLNIGEEAFEPDGPISDRPMSNLEKVHFIVGYAILRPNLRDEIYCQICKQLSENFKTSCLARGWILLSLCLGCFPPSERFMKYLLNFIGQGPAGYGPFCAERLRRTYANGVRTEPPTWLELQAVKSKKHIPIHVILVTGESLTVMVDSASTSREVCLHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHVMDTVAQCEQLARDRGENERQSPWRIYFRKEFFTPWHDSQEDPVSTQLIYRQVLHGVWSGEYNFEKEDDLVQLLAKHCYVQLGASVGSEAVQELLPSCIPSKLYKTKPPEKWASLVTAAHAKAQYTQKQAKPLAVQEQVVDTARLQWPLLFSRLFEVTTLSGPRLPKTQLILAVNWKGMYFLDQREKILLELSFPEVVSLITNREAQGGQRLVLSTLHEEEYKFMSPSSVAIAELVAMFLEGLKERSVFAMALQNRKATDDTTILPFKKGDLLILTKKQGLLASENWTLGQNDRTGKTGLVPTACLYTIPTVTKPSAQLMSLLAMSPEKRKLAAQEVQPTEPLPEEQPKEKPHTLEEFSYEFFRAPEKETVRRTMLPLARTQGHLWAHSSEPLRQPLLKQVHANAELRDPACQIFIGILRYMGDYPSRQAWTSVELADQIFSLALQESALRDEVYCQILKQLTHNTTRYSEERGWQLLWLCTGLFPPGKALLPHAQKFVDTRRKKLLAPDCSRRIQRILRTGPRKQPPHPVEVEAIEQNISRICHKIYFPNDTSEMLEVGTYTRVRDVCQGIATRLQLSSWEGCSLFIKIADKVISQKEGDFFFDSLRQVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNVTPGKDVNADTVLHYHQELPKYLRGFHKCSQEDAVHLAGLIYKAQFDNNRSQLASIPKILRELVPENLTRLMSSEEWRKNILLAYDKHKDKTVEEAKVAFLKGICRWPTFGSAFFEVKQTSEPSYPDIILIAINRHGVLLIHPKTKELLTTYPFTKISSWSSGSTYFHMALGSLARGSRLLCETSLGYKMDDLLTSYVQQLLSAMNKQRGSQTPALADP

Target ORF information:

RefSeq Version	XM_014121387.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin VIIB (MYO7B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121387.1

ORF Insert Sequence:

ATGGTCCAGC CTCCCTTGAG AGAACTTCTG GGAGCCAGGA TTCAGGCTGG GAGGACCTGC 
TGCCTCAGGA TGTCAGCCTT CAGGCTGGGT GATCATGTGT GGCTAAACCC CCTCTCTGCC 
AACAAGACCA GTGTGGCCAT TGGATCCATT GTCAAGGAAA CAAAGCCTGG CAAGATCTTG 
GTTGTAGATG ATGAAGGCAA GGAACACTGG ATCCAAGCAA AAGACCTTGA CACCCTCAGC 
CCCATGCACC CCAACTCAGT GCAAGGCGTG GATGACATGA TCCGCCTGGG GGAACTGAAC 
GAGGCAGGCA TGGTGCACAA CCTCCTGATC CGCTACCGGC AGCACAAGAT CTATACATAC 
ACAGGCTCCA TTCTGATGGC CGTGAACCCC TTCCAAGTGT TGCCCCTCTA CACCCTGGAG 
CAGGTACAGC TGTACTACAA CCGTCACATG GGCGAACTAC CACCCCATGT CTTTGCCATC 
GCCAACAACT GCTACTTCAA CATGAAGAGG AACAAGAGGG ACCAGTGCTG CATTATCAGT 
GGTGAGTCTG GGGCAGGGAA GACAGAGACC ACGAAGCTCA TCCTGCAGTT CCTGGCCACC 
GTCAGTGGCC AACATTCCTG GATTGAGCAG CAGGTCTTGG AGGCCAACCC CATCCTGGAA 
GCCTTCGGAA ATGCCAAGAC AGTCAGAAAT GATAACTCAA GCCGCTTTGG GAAATATATC 
GACATCCACT TCAACCCCAA TGGGGTGATT GAGGGCGCAC GCATCGAGCA ATTTCTCCTG 
GAGAAGTCCC GGGTCTGCCG GCAGGCTCCC GAGGAGCGGA ACTACCACAT CTTCTACGGC 
ATGCTCCTGG GGATGACCGC GGAGGAGAAG AAGCTGCTGA GCCTGGGGAC GCCCTCCGAG 
TACCACTACC TGACCACGGG AAACTGCACA TCCTGTGAGG GCCTCAATGA TGCCAAGGAC 
TATGCCCATG TTCGCTCTGC CATGAAGATC CTCATGTTCT CCGACTCTGA GAACTGGGAC 
CTCAGCAAGC TGCTGGCAGC CATTCTCCAC CTGGGCAATG TGGAGTTTAT GGCTGCAGTC 
TTCGAGAACC TGGATTCCTC CGACGTGATG GAGACGCCTG CCTTTCCCAC CGTGCTGAAG 
TTACTGGAGG TGGAGTACCA GGCACTCCGG GACTGTCTGA TCAAGCATTC CATCATCATC 
CGAGGGGAGT TTGTCACCAG GCCCCTGAAC ATCACTCAGG CTGCAGACCG GAGGGATGCC 
TTTGTCAAGG GCATCTATGG GCACCTCTTC CTGTGGATTG TCAAGAAGAT CAACACTGCA 
ATTTTCACTC CACCTGCCCA GGACCCCAAA AATGTGCGAA GAGCCATTGG CCTCCTGGAC 
ATATTTGGCT TTGAAAATTT CCAGAACAAT AGCTTCGAGC AGCTCTGCAT CAACTTTGCC 
AACGAGCACC TGCAGCAGTT CTTCGTGCAG CATGTGTTCA CCATGGAGCA GGAGGAGTAC 
CTCTCAGAGA ACATTGCCTG GGACTACATC CACTACAATG ACAATCGGCC CACCCTGGAT 
CTGCTGGCCC TTAAGCCCAT GAGCATCATC TCCCTCCTGG ACGAGGAGAG CCACTTCCCA 
CAGGGGACAG ATACAACCAT GCTGCAGAAA CTGAACAGTG TCCATGCCAA CAATAAGGCC 
TACCTACAGC CCAGGAACAT CCATGATGCC AGGTTTGGCA TCGCCCACTT TGCAGGCGAG 
GTGTACTACC AGACAGAAGG CTTCCTGGAG AAGAATCGAG ACATGCTGAG CACAGATATC 
CTCACTCTGG TCTACTCCTC CAAAAACAAG TTCCTGAAGG AGATATTCAA GCTGGAGTCA 
GCGGGGACCA AGCTGGGCCA AAGCACCATC ATCCAGGCTA GCGCAGGGAG CCAGCTCTTC 
AAGTCTGCAG ACTCCAGCAA GCAGCCCTCC ACATTGGCAG GCCAGTTCAA GAAGTCCCTG 
GACCAGCTGA TGAAAATCCT GACCAGCTGC CAGCCCTACT TTATCCGCTG CATCAAACCC 
AATGAGTACA AGAAGCCACT GCTCTTCGAC CGCGACCTGT GCCTCCGGCA GCTGCGTTAC 
TCGGGGATGA TGGAGACCGT GCACATCCGC AGGTCCGGCT TCCCCATCCG GTACACGTTC 
CAGGAGTTCG CGCAGAGGTT CGGCGTGCTG CTGCCCAGCG CCGTTCGCCT GCAGCTTCGA 
GACAAGGCTC GCCAGATGAC CCTGCGCATT GCTGAAATGC AGCTGGGGAC GGACAAAGAA 
TGGAAAGTGG GAAAGACCAA AATTTTCCTG AAGGATAACC AGGACACTCT ACTTGAGGTA 
CAGAGGACCC AGACCCTGGA CAAGGCAGCA ATCAACATCC AAAGAGTACT GCGAGGCTAC 
AAATACAGGA AGGAGTTCTT GAGACAGAGG CGGGCCGCTG TGACCCTCCA GGCCAGGTGG 
AGAGGCTACT ACAACAAGCG GAACTTCAAG CAGATCCTCT TGGGTTTTGA GCGCCTGCAG 
GCCATTGCCC GGAGCCAGTG GCTAGCAAAG CAGTACCAGA CCATGCGGCA GAGGATGGTC 
CAGCTGCAGG CACTCTGCAG GGGCTACCTT GTGCGCCAGC AAGTCCAGGC CAAGAGAAGG 
GCAGTGGTGG TCATACAGGC ACATGCCAGG GGCATGGCTG CGCGGCGGAA CTTCCAGCAG 
CAGAAAGCCA ATGGGCCTCT GGTCATCCCA GCTGAAGGGC AGAAGAGCCA AAGTATTCTC 
CCCACCAGGA AGCGTAAGTC AATCTACGAT ACCATCACTG ACACGGAGAT GGTGGAGCAA 
GTGTTTGGCT TCCTCCCTTC CATGATTGGT GGGCAGGAGG GCCAGGCTCC ATGGCGCTTT 
GAGGACCTGG AAGCGAAGAG CCAGAAGCTG CCCGAGGTTG ACCTGGACAC GGTCCCCCTG 
GTGGAGGAGT CTGAGGACGA TGTGGACGGG CTGGCCGAGT ACACCTTCCC CAAGTTTGCT 
GCCACCTACT TCCAGAAATC GGCCAGCCAC ACGCACATCC GGCGGCCCCT CCGATACCCA 
CTGCTCTACC ATGAGGAAGA TGCTGACTGC TCGGCCGCCC TGGCCGTGTG GAACATCATT 
CTGAGATTCA TGGGTGACCT TCCAGAGCCA GTGCTTTTTG CCAGGAATGG CCCGCCCGGC 
GGCTCCGTGA CGCGGCAGAT CCGTGACAGC CTGGGCAGGG AGGGCGGCAC CCGGTCCCCA 
CCGCACTGCG GGTCCACACA GAAGTCCAGC CGCAGAGACA GAGGGAGCAA GGACATCTCC 
TCCATGAAGC TAAAGCGGTC CTCCAAGATC ACAAGCCAGG TAACCAGCCA GCTGAATATT 
GGAGAGGAGG CATTTGAGCC TGATGGCCCC ATCTCAGACC GACCCATGTC CAATCTGGAG 
AAGGTACACT TCATTGTGGG CTATGCTATC CTGCGGCCTA ACCTCAGGGA CGAGATTTAC 
TGCCAGATCT GCAAGCAGCT GTCCGAGAAC TTCAAAACAA GCTGCCTGGC CCGGGGCTGG 
ATCCTGCTCA GCCTTTGCTT GGGCTGTTTC CCACCCTCCG AGAGGTTCAT GAAGTATCTG 
CTAAACTTCA TTGGTCAAGG GCCAGCTGGC TATGGGCCCT TCTGTGCAGA ACGCCTGAGA 
CGAACCTATG CTAATGGGGT ACGCACGGAG CCCCCCACGT GGCTGGAGCT GCAGGCAGTC 
AAATCCAAGA AGCACATCCC AATCCATGTC ATCTTGGTGA CCGGAGAGAG CCTGACTGTC 
ATGGTTGACT CTGCCTCGAC ATCTCGGGAA GTCTGCCTGC ACATTGCCCA CAAGCAGGGC 
CTCAGCGATC ACCTGGGCTT TTCTCTCCAG GTGGCCGTGT ACGACAAGTT CTGGTCCCTG 
GGCAGCGGGC GCGACCACGT GATGGATACA GTCGCCCAGT GTGAGCAGCT GGCCCGGGAC 
AGGGGGGAGA ACGAGCGCCA GTCGCCCTGG CGCATCTACT TCCGGAAGGA GTTCTTCACC 
CCCTGGCATG ACTCCCAGGA GGACCCCGTC AGTACCCAGC TCATCTACCG CCAAGTGCTG 
CATGGGGTCT GGTCTGGCGA GTACAACTTC GAGAAGGAGG ATGATCTGGT ACAGCTGCTG 
GCCAAGCACT GCTACGTGCA GCTCGGTGCC TCGGTGGGGA GTGAAGCTGT CCAGGAGCTG 
CTGCCCAGCT GCATCCCCTC CAAGCTGTAC AAGACCAAGC CTCCAGAGAA GTGGGCTAGC 
CTCGTCACTG CTGCCCACGC CAAGGCACAG TACACCCAGA AGCAGGCAAA GCCGCTGGCT 
GTGCAGGAGC AGGTGGTGGA CACGGCCCGC CTGCAGTGGC CGCTGCTCTT CTCCCGGCTC 
TTTGAGGTCA CCACGCTCTC CGGCCCCCGA CTGCCCAAGA CTCAGCTGAT CTTAGCTGTT 
AACTGGAAGG GGATGTACTT CCTGGACCAG AGAGAGAAGA TACTCCTAGA ACTCTCTTTT 
CCAGAGGTCG TGAGCCTGAT CACCAACAGA GAGGCGCAGG GTGGGCAGAG GCTGGTACTC 
TCCACGCTGC ACGAAGAGGA GTACAAGTTC ATGTCCCCCA GCAGCGTGGC CATCGCCGAG 
CTGGTAGCTA TGTTTCTGGA AGGCTTGAAG GAGAGGTCCG TGTTCGCCAT GGCTCTGCAG 
AACAGGAAGG CCACAGATGA CACCACCATC CTGCCCTTCA AGAAGGGGGA CCTGCTGATC 
CTGACAAAGA AGCAGGGGTT GTTGGCCTCT GAGAACTGGA CCTTGGGCCA GAACGACAGG 
ACAGGCAAGA CGGGGCTGGT GCCCACAGCT TGCCTCTACA CCATCCCAAC GGTCACCAAG 
CCCTCGGCAC AGCTGATGAG TTTGCTGGCC ATGTCACCAG AGAAGCGGAA GCTGGCAGCC 
CAGGAGGTGC AGCCCACAGA GCCACTGCCT GAGGAGCAGC CCAAGGAGAA GCCACACACC 
CTGGAGGAAT TCTCCTACGA GTTCTTTAGG GCCCCAGAGA AGGAGACAGT CAGGAGGACC 
ATGTTGCCCC TGGCCCGGAC ACAGGGCCAC CTGTGGGCCC ACTCATCCGA GCCACTGCGG 
CAGCCTCTGC TCAAGCAAGT CCACGCCAAT GCCGAGCTCC GGGACCCTGC CTGTCAGATC 
TTCATTGGCA TCCTCCGGTA CATGGGTGAC TACCCATCTC GACAGGCTTG GACCTCCGTG 
GAGCTCGCCG ACCAGATCTT CTCGTTGGCC CTCCAGGAGT CAGCCCTGCG GGATGAGGTC 
TACTGCCAGA TCCTGAAGCA GCTGACACAC AACACCACGA GGTACAGTGA GGAGCGGGGC 
TGGCAGCTGC TGTGGCTCTG CACTGGCCTC TTCCCCCCGG GAAAGGCGCT GCTGCCTCAT 
GCTCAGAAGT TCGTAGACAC GCGGAGGAAG AAGCTATTGG CTCCCGACTG CAGCCGGCGC 
ATCCAGAGGA TCCTGAGGAC AGGACCCCGG AAGCAGCCCC CACACCCAGT GGAGGTGGAG 
GCCATAGAGC AGAACATCTC TCGCATCTGC CACAAGATCT ACTTCCCTAA CGACACTAGC 
GAGATGCTGG AGGTGGGCAC CTACACTCGG GTGCGAGATG TATGCCAGGG CATCGCCACC 
AGGCTGCAGC TGTCTTCCTG GGAAGGCTGC AGCCTCTTCA TCAAGATTGC TGACAAGGTC 
ATCAGCCAGA AGGAGGGAGA CTTCTTCTTT GACTCCTTGC GGCAAGTGTC TGATTGGGTG 
AAGAAAAACA AGCCCCAGAA AGAAGGGGCG CCCGTGACAC TCCCCTACCA GGTGTACTTC 
ATGCGGAAAT TGTGGCTCAA CGTGACCCCG GGGAAGGATG TGAATGCAGA CACCGTACTT 
CATTACCATC AGGAGCTGCC CAAGTACCTT CGTGGATTCC ACAAGTGTTC ACAGGAGGAT 
GCTGTCCACC TGGCCGGCCT CATCTACAAG GCCCAGTTTG ACAACAACCG GTCCCAGCTA 
GCTAGTATCC CCAAGATCCT GAGGGAGCTT GTGCCTGAGA ACCTGACACG TCTCATGTCC 
TCCGAGGAAT GGAGAAAGAA CATCCTTCTG GCCTATGACA AGCACAAGGA CAAGACAGTA 
GAGGAGGCCA AGGTGGCGTT CCTCAAGGGG ATCTGCCGGT GGCCCACATT TGGGTCTGCC 
TTCTTCGAGG TGAAGCAAAC CTCGGAGCCT TCCTACCCAG ACATCATCCT CATCGCCATC 
AACCGGCACG GGGTTCTGCT TATCCACCCC AAGACCAAGG AGCTGCTCAC CACCTACCCC 
TTCACCAAGA TCTCCAGCTG GAGCAGCGGC AGCACCTACT TCCACATGGC ACTGGGGAGC 
CTGGCCCGGG GCAGCCGCCT GCTGTGTGAA ACCTCTCTGG GCTACAAGAT GGATGACCTG 
CTGACCTCGT ATGTGCAGCA GCTCCTGAGC GCCATGAACA AGCAGCGGGG CTCCCAGACC 
CCGGCCCTGG CTGATCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

MYO7B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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