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HEG1 cDNA ORF clone, Canis lupus familiaris(Dog)

Summary

Gene Symbol HEG1
Entrez Gene ID 488015
Full Name heart development protein with EGF like domains 1
General protein information
Preferred Names

heart development protein with EGF like domains 1

Names

protein HEG homolog 1
HEG homolog 1
Gene Type protein-coding
Organism Canis lupus familiaris(Dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_022414008.1 XP_022269716.1 protein HEG homolog 1
XM_014110221.1 XP_013965696.1 protein HEG homolog 1

Pathways from BioSystems


Homologies


Homo sapiens (human) HEG1 NP_065784.1
HEG1 XP_002802724.1
Bos taurus (cattle) HEG1 XP_002684860.1
Mus musculus (house mouse) Heg1 NP_780465.4
Rattus norvegicus (Norway rat) Heg1 XP_006248505.1
Pan troglodytes (chimpanzee) HEG1 XP_516708.4
Canis lupus familiaris (dog) HEG1 XP_005639641.1

Gene Ontology

The following HEG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe44718 XM_014110221.1
Latest version!		 Canis lupus familiaris heart development protein with EGF-like domains 1 (HEG1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $797.30
$1139.00
OCe284336 XM_022414008.1
Latest version!		 Canis lupus familiaris heart development protein with EGF like domains 1 (HEG1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe44718
Clone ID Related Accession (Same CDS sequence) XM_014110221.1
Accession Version XM_014110221.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product protein HEG homolog 1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAACAGTT GGCCAGAAAG TAACACTGAA CCACACGTAG AAAACGGCAC CTTTGATCAG 
AATCAAGTGG ACTTTTCAAC AGTGGTTTCC AAGGAGGGTG TGATGACTCA GACCTCCAGA 
AAGAGCCACA CTTCTTCAAA TGCTCCAGAA AACCTCACTC CACAAACAGA AGCGGCAGAT 
GCTAGAGGAA GAAACGACAC TTCCAGGAAA ACAAATTTCA CTGTTTCCCC TGTTGGGCCT 
GACGTAGCAA CAGCTCTGAC TTCCCAGAGT ATCACCACGG CTTTGGGAAT GCTTCACTTG 
CCATCCAGCT CAGGGTCCGA AGGAAGAACT GCCCCTTCTC ACACGGAGAG CACAACATCC 
CGGGGTTCCC TGGCGAGACG AAAGGGGCTC CCAGACGAAG TGACTGTGCA CACCCAAGTG 
GCTGCCACTT GGGTCTTAGG CCAGTTCCCT CTTCCTGCTT TGGAGACTGG AGAAGAGACC 
ACACTCTCCA GGAAGAGGAA CACCTCAGGA CTGGCGCTCT CCTGGCCACA TTTCTCCAGG 
ACACCAGCTT ACTCCTCTCC CTTGGAGCCC TCCTCGGCTA CTAGGAGCAC AGAGGAGCTC 
AACGACTCCA CTGTTCTCCC GAGTGCTTTG GTGAGTTGGA CAGAGAGTGT GCGTGTTGCT 
GCCACGTTCC CCAGCGGTGT CCCCGGGAGG CTCCAGTCTG TAAGCGTCAG TCTGCAACCT 
CTGAATGAGA CAGAGAGTTT CCCCAAGGAC TCGGAAATTG CCACGACTTC ACTATCAAAC 
CATTCCTCAT CCTTGGAGGC AGAGTTTCAA GGAACCAAGG AGGTCACCAG GAAGCCAGGG 
GCTGGAGAAT CCATTGAGCC ATCCACAGAG AATGGATTTG GCCCCATATC TTCAGGGGTC 
TCAGTGAGAT TTTGGCAGAA TGATTCCCCA ACCTCGGGAG GGGACCAGCC GGCCAGCAGC 
TCGGAGGCGG GGAGCGGAAG GCCCGCGGCC CGCACCGAGG CCGTGTCTCA GTCGGACCCG 
TTGGGCAGAA GCGGAGACAG CGCGGCGCGC CGGGCCTTGA CCAGCAGCCA CACCGCCGAG 
GCCGTGAGCG GCGGCTCCAC TTCCCAGCCC GCCGCCCCCC TCTCCTCCTC CGGCCCCCGC 
TCTAGGGGAG GCGACCCGGC GCTGGGCACC AGGAGCCACG CTGGGCCGGC CCCCGAGTCT 
TTGTCGTCCT CCTCCTCAGA GAGCCTGGCT GCCTCGGCGC CGCGTGACGA GCGCTCCACC 
ACCGAGGACG GTGCAGAGCC CGGAGGCAGT TCGGACGTGG AGCAGAGGAC GGCGCAGGCC 
CACCCCCCGC ACACGGCTGC CACCTTCACC CGGAACGGGG AGCGCACGCT GCGGTCTCTC 
GCCAACGCCA GCACGACTTC TGGGGACGTG GGGCGCTCTG CCGTCGTGCC CGGGGAGACC 
GACGGCGCCA CCCCGCAAGG GGACGTGCCC GTGACTGGTG ACGCCCGCCT GGTCTCGGGT 
TCACGGGCGG CCTCGCCAGC CCCGGGAGTC ACCGGAGTCA GCGACAGTGG AGTGAGCGGC 
ACGGACACTG AACAGAGGAC TTCCGGTGAC CACACAGACC ACACCTATGT CTCATCCACT 
TTCACCAAAG GAGAACGGGT GTTGCTTTCC ATTATAGACA ACAGCTCATC GGCGGACTTA 
CCGGAGAGTT CCACCTCTGC TGCTAGGATC TCCAACTCTT CACACTCAGA CCATTCCTCC 
TCTTCTCAGG CTCAGACTGA AAGAAGTAAC GTGTCATCCT ATGAAAGGGA GCGTGCTCAG 
CCCTCCACGG AGTCGCTGGC TCTGAGAACA GCCAACGTCC CATCCTCCAC ACCCACCGTT 
AACATGCCAA ACACTTTGGT TCTTCTGGAC ACTGATTCTT CTTCTGTTTC TTCTTCTGTT 
TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT TCTTCAGGAC CCCTTTCACC CCTGGCCTCA 
GTGTCCCAGG CCCATCAATT GTTGTCATCA GTCTTACCAT CAATCGGGGC CTCCACCCTT 
CCACTGCAGT CCACTCCCGA GGCGCCCACA CCTATGTCTT CCTCGCCACC ACCCTTACCA 
ATATCCCTGA CAGCGTCTAC CCCTGCGTCA CCATCTGCCT CCCAAACAAC CCTCCCACCT 
TCACCTTCTA CCCTGGTCCT CCCCAGAACA AGAGACACTC CTGTGACTTC GGTTCAGATG 
GAGACAGTGG CATTATCTGT GGCAATGCTC CCCAGTACTC AAACGGCAGA TCCTAAGAAC 
CAGAGTAACC CATACCATGA CAAAGTCATT ACAGAGTCAA AGCCACCAAG CCTGGCATCT 
CTGCCCACAG AGGCCACCGG AGCTGTAACA ACGAGGTCTA CTTCAGGGTT CCCTACGCCC 
TCGACCTTAA CAGATTCCTC CACTCTGCAA ACCCTTGCAG CCACGAGCAC CAGCATCACC 
CCGACATCTC CAGCATTGAC AACCACCGTT CTTGAGACCT CTCGTCCCTT CAGCCCTGCG 
TCAACCACAG CAGCTCTGAT GGCTCACCCC ACAACAGTAC AGACCACGGC TGAAAAACAG 
CCCTTGACAA CCAGCCCTGA AATTCTGGCA CCACGGAGCT CAACAGAAAG TGCCATCACC 
ACAGAAAGGA GCCAGATCCA TGTCGATGGT ACCAGCCCGT TGACCCCCCT GACCAGCACA 
CCCACATCAC TGAAAGGATT GACCCCAGGG CCTGGTATGA CCAAAGAGTA CAGCCCGGCT 
TCACATTTCC TCAGAACATC TCCCCAAACC ACAGGCGTTT CCACGCTCGA AGTACTGCCT 
CCTGAATCTA CCACCTTTGC TGCTCAGAGC AGCACGCAGC CACCAAAAGC ATTGTCATCT 
CCAGCCTCAG TTAACAGCTG CGCCACTAAC CCTTGTCTTC ATGATGGGAA ATGCATTGTG 
GACTCCACCA GCCATGGGTA TCGATGCGAA TGCTCACCTT CCTGGCAAGG GGATGATTGC 
AGTGTGGATG TGAATGAATG CCTCTTGAGC CCCTGCCCAC CCCTGGCCAC GTGCAATAAT 
ACTCAGGGAT CCTTCACCTG CAAATGCCCA GTTGGGTACC AGCTGGAAAA AGGAATATGC 
AATTTGGTTA GAACCTTCGT GACAGAGTTC AAATTAAAGA AAACATTTCT CAATACCACC 
ATGGAGAAAC ACGCAGACCT ACATGAAGTT GAAAATGAGA TCACCAAAAC ATTAAATATG 
TGTTTTTCAA CGTTACCTGG TTACACCCGA TCCACAGTTC ATGCTTCTAG GGAGTCCAAT 
GTGGTGGTAA TGTCCTTGAA AACCACTTTT TCTTTGGCTT CAAAGGTGAC ACTGTTTGCC 
TTGGCCGAGG GGATGCAGAA ATGTGTCAAT TCCTGTAGTT CTTCTGCGGA GGCTTGCAAA 
TTCTTGGGAT TTCAGAGGGG GATCTTTAAA GCGGGCAGCC TGTGCAAGCG GAAGACTCCA 
GAATGTGACA GAGAGACCTC CATCTGCACA GACCTGGACG GCGTCGCCCT GTGCCAATGC 
AAGTCGGGCT ACTTCCAGTT CAACAAGATG GATCACTCCT GCCGAGCATG CGAAGATGGA 
TATAGGCTTG AAAATGAAAC CTGTATGAGT TGCCCATTTG GGCTTGGTGG TCTCAACTGT 
GGAAACCCCT ATCAGCTGAT CACCGTAGTG ATCGCAGCAG CAGGAGGAGG GCTTCTACTT 
ATTCTGGGCA TCGCACTGAT TGTCACCTGC TGCCGAAAGA GTAAAAATGA CATAAGCAAA 
CTCATCTTCA AAAGTGGGGA TTTCCAAATG TCCCCATATG CTGAGTACCC CAAAAACCCT 
CGCTCACAAG AATGGGGCCG AGAAGCCATT GAAATGCATG AGAACGGAAG CACCAAAAAC 
CTCCTCCAGA TGACGGATGT GTATTACTCG CCCACAAGTG TAAGGAATCC TGAACTTGAA 
CGAAATGGAC TGTACCCGGC CTACACTGGA CTGCCGGGGT CCCGGCACTC GTGCATTTTC 
CCCGGACAGT ATAACCCGTC TTTCATCAGT GATGAGAGCA GGAGAAGAGA CTACTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013965696.1
CDS27..4106
Translation

Target ORF information:

RefSeq Version XM_014110221.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris heart development protein with EGF-like domains 1 (HEG1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014110221.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAACAGTT GGCCAGAAAG TAACACTGAA CCACACGTAG AAAACGGCAC CTTTGATCAG 
AATCAAGTGG ACTTTTCAAC AGTGGTTTCC AAGGAGGGTG TGATGACTCA GACCTCCAGA 
AAGAGCCACA CTTCTTCAAA TGCTCCAGAA AACCTCACTC CACAAACAGA AGCGGCAGAT 
GCTAGAGGAA GAAACGACAC TTCCAGGAAA ACAAATTTCA CTGTTTCCCC TGTTGGGCCT 
GACGTAGCAA CAGCTCTGAC TTCCCAGAGT ATCACCACGG CTTTGGGAAT GCTTCACTTG 
CCATCCAGCT CAGGGTCCGA AGGAAGAACT GCCCCTTCTC ACACGGAGAG CACAACATCC 
CGGGGTTCCC TGGCGAGACG AAAGGGGCTC CCAGACGAAG TGACTGTGCA CACCCAAGTG 
GCTGCCACTT GGGTCTTAGG CCAGTTCCCT CTTCCTGCTT TGGAGACTGG AGAAGAGACC 
ACACTCTCCA GGAAGAGGAA CACCTCAGGA CTGGCGCTCT CCTGGCCACA TTTCTCCAGG 
ACACCAGCTT ACTCCTCTCC CTTGGAGCCC TCCTCGGCTA CTAGGAGCAC AGAGGAGCTC 
AACGACTCCA CTGTTCTCCC GAGTGCTTTG GTGAGTTGGA CAGAGAGTGT GCGTGTTGCT 
GCCACGTTCC CCAGCGGTGT CCCCGGGAGG CTCCAGTCTG TAAGCGTCAG TCTGCAACCT 
CTGAATGAGA CAGAGAGTTT CCCCAAGGAC TCGGAAATTG CCACGACTTC ACTATCAAAC 
CATTCCTCAT CCTTGGAGGC AGAGTTTCAA GGAACCAAGG AGGTCACCAG GAAGCCAGGG 
GCTGGAGAAT CCATTGAGCC ATCCACAGAG AATGGATTTG GCCCCATATC TTCAGGGGTC 
TCAGTGAGAT TTTGGCAGAA TGATTCCCCA ACCTCGGGAG GGGACCAGCC GGCCAGCAGC 
TCGGAGGCGG GGAGCGGAAG GCCCGCGGCC CGCACCGAGG CCGTGTCTCA GTCGGACCCG 
TTGGGCAGAA GCGGAGACAG CGCGGCGCGC CGGGCCTTGA CCAGCAGCCA CACCGCCGAG 
GCCGTGAGCG GCGGCTCCAC TTCCCAGCCC GCCGCCCCCC TCTCCTCCTC CGGCCCCCGC 
TCTAGGGGAG GCGACCCGGC GCTGGGCACC AGGAGCCACG CTGGGCCGGC CCCCGAGTCT 
TTGTCGTCCT CCTCCTCAGA GAGCCTGGCT GCCTCGGCGC CGCGTGACGA GCGCTCCACC 
ACCGAGGACG GTGCAGAGCC CGGAGGCAGT TCGGACGTGG AGCAGAGGAC GGCGCAGGCC 
CACCCCCCGC ACACGGCTGC CACCTTCACC CGGAACGGGG AGCGCACGCT GCGGTCTCTC 
GCCAACGCCA GCACGACTTC TGGGGACGTG GGGCGCTCTG CCGTCGTGCC CGGGGAGACC 
GACGGCGCCA CCCCGCAAGG GGACGTGCCC GTGACTGGTG ACGCCCGCCT GGTCTCGGGT 
TCACGGGCGG CCTCGCCAGC CCCGGGAGTC ACCGGAGTCA GCGACAGTGG AGTGAGCGGC 
ACGGACACTG AACAGAGGAC TTCCGGTGAC CACACAGACC ACACCTATGT CTCATCCACT 
TTCACCAAAG GAGAACGGGT GTTGCTTTCC ATTATAGACA ACAGCTCATC GGCGGACTTA 
CCGGAGAGTT CCACCTCTGC TGCTAGGATC TCCAACTCTT CACACTCAGA CCATTCCTCC 
TCTTCTCAGG CTCAGACTGA AAGAAGTAAC GTGTCATCCT ATGAAAGGGA GCGTGCTCAG 
CCCTCCACGG AGTCGCTGGC TCTGAGAACA GCCAACGTCC CATCCTCCAC ACCCACCGTT 
AACATGCCAA ACACTTTGGT TCTTCTGGAC ACTGATTCTT CTTCTGTTTC TTCTTCTGTT 
TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT TCTTCAGGAC CCCTTTCACC CCTGGCCTCA 
GTGTCCCAGG CCCATCAATT GTTGTCATCA GTCTTACCAT CAATCGGGGC CTCCACCCTT 
CCACTGCAGT CCACTCCCGA GGCGCCCACA CCTATGTCTT CCTCGCCACC ACCCTTACCA 
ATATCCCTGA CAGCGTCTAC CCCTGCGTCA CCATCTGCCT CCCAAACAAC CCTCCCACCT 
TCACCTTCTA CCCTGGTCCT CCCCAGAACA AGAGACACTC CTGTGACTTC GGTTCAGATG 
GAGACAGTGG CATTATCTGT GGCAATGCTC CCCAGTACTC AAACGGCAGA TCCTAAGAAC 
CAGAGTAACC CATACCATGA CAAAGTCATT ACAGAGTCAA AGCCACCAAG CCTGGCATCT 
CTGCCCACAG AGGCCACCGG AGCTGTAACA ACGAGGTCTA CTTCAGGGTT CCCTACGCCC 
TCGACCTTAA CAGATTCCTC CACTCTGCAA ACCCTTGCAG CCACGAGCAC CAGCATCACC 
CCGACATCTC CAGCATTGAC AACCACCGTT CTTGAGACCT CTCGTCCCTT CAGCCCTGCG 
TCAACCACAG CAGCTCTGAT GGCTCACCCC ACAACAGTAC AGACCACGGC TGAAAAACAG 
CCCTTGACAA CCAGCCCTGA AATTCTGGCA CCACGGAGCT CAACAGAAAG TGCCATCACC 
ACAGAAAGGA GCCAGATCCA TGTCGATGGT ACCAGCCCGT TGACCCCCCT GACCAGCACA 
CCCACATCAC TGAAAGGATT GACCCCAGGG CCTGGTATGA CCAAAGAGTA CAGCCCGGCT 
TCACATTTCC TCAGAACATC TCCCCAAACC ACAGGCGTTT CCACGCTCGA AGTACTGCCT 
CCTGAATCTA CCACCTTTGC TGCTCAGAGC AGCACGCAGC CACCAAAAGC ATTGTCATCT 
CCAGCCTCAG TTAACAGCTG CGCCACTAAC CCTTGTCTTC ATGATGGGAA ATGCATTGTG 
GACTCCACCA GCCATGGGTA TCGATGCGAA TGCTCACCTT CCTGGCAAGG GGATGATTGC 
AGTGTGGATG TGAATGAATG CCTCTTGAGC CCCTGCCCAC CCCTGGCCAC GTGCAATAAT 
ACTCAGGGAT CCTTCACCTG CAAATGCCCA GTTGGGTACC AGCTGGAAAA AGGAATATGC 
AATTTGGTTA GAACCTTCGT GACAGAGTTC AAATTAAAGA AAACATTTCT CAATACCACC 
ATGGAGAAAC ACGCAGACCT ACATGAAGTT GAAAATGAGA TCACCAAAAC ATTAAATATG 
TGTTTTTCAA CGTTACCTGG TTACACCCGA TCCACAGTTC ATGCTTCTAG GGAGTCCAAT 
GTGGTGGTAA TGTCCTTGAA AACCACTTTT TCTTTGGCTT CAAAGGTGAC ACTGTTTGCC 
TTGGCCGAGG GGATGCAGAA ATGTGTCAAT TCCTGTAGTT CTTCTGCGGA GGCTTGCAAA 
TTCTTGGGAT TTCAGAGGGG GATCTTTAAA GCGGGCAGCC TGTGCAAGCG GAAGACTCCA 
GAATGTGACA GAGAGACCTC CATCTGCACA GACCTGGACG GCGTCGCCCT GTGCCAATGC 
AAGTCGGGCT ACTTCCAGTT CAACAAGATG GATCACTCCT GCCGAGCATG CGAAGATGGA 
TATAGGCTTG AAAATGAAAC CTGTATGAGT TGCCCATTTG GGCTTGGTGG TCTCAACTGT 
GGAAACCCCT ATCAGCTGAT CACCGTAGTG ATCGCAGCAG CAGGAGGAGG GCTTCTACTT 
ATTCTGGGCA TCGCACTGAT TGTCACCTGC TGCCGAAAGA GTAAAAATGA CATAAGCAAA 
CTCATCTTCA AAAGTGGGGA TTTCCAAATG TCCCCATATG CTGAGTACCC CAAAAACCCT 
CGCTCACAAG AATGGGGCCG AGAAGCCATT GAAATGCATG AGAACGGAAG CACCAAAAAC 
CTCCTCCAGA TGACGGATGT GTATTACTCG CCCACAAGTG TAAGGAATCC TGAACTTGAA 
CGAAATGGAC TGTACCCGGC CTACACTGGA CTGCCGGGGT CCCGGCACTC GTGCATTTTC 
CCCGGACAGT ATAACCCGTC TTTCATCAGT GATGAGAGCA GGAGAAGAGA CTACTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe284336
Clone ID Related Accession (Same CDS sequence) XM_022414008.1
Accession Version XM_022414008.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product protein HEG homolog 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAACAGTT GGCCAGAAAG TAACACTGAA CCACACGTAG AAAACGGCAC CTTTGATCAG 
AATCAAGTGG ACTTTTCAAC AGTGGTTTCC AAGGAGGGTG TGATGACTCA GACCTCCAGA 
AAGAGCCACA CTTCTTCAAA TGCTCCAGAA AACCTCACTC CACAAACAGA AGCGGCAGAT 
GCTAGAGGAA GAAACGACAC TTCCAGGAAA ACAAATTTCA CTGTTTCCCC TGTTGGGCCT 
GACGTAGCAA CAGCTCTGAC TTCCCAGAGT ATCACCACGG CTTTGGGAAT GCTTCACTTG 
CCATCCAGCT CAGGGTCCGA AGGAAGAACT GCCCCTTCTC ACACGGAGAG CACAACATCC 
CGGGGTTCCC TGGCGAGACG AAAGGGGCTC CCAGACGAAG TGACTGTGCA CACCCAAGTG 
GCTGCCACTT GGGTCTTAGG CCAGTTCCCT CTTCCTGCTT TGGAGACTGG AGAAGAGACC 
ACACTCTCCA GGAAGAGGAA CACCTCAGGA CTGGCGCTCT CCTGGCCACA TTTCTCCAGG 
ACACCAGCTT ACTCCTCTCC CTTGGAGCCC TCCTCGGCTA CTAGGAGCAC AGAGGAGCTC 
AACGACTCCA CTGTTCTCCC GAGTGCTTTG GTGAGTTGGA CAGAGAGTGT GCGTGTTGCT 
GCCACGTTCC CCAGCGGTGT CCCCGGGAGG CTCCAGTCTG TAAGCGTCAG TCTGCAACCT 
CTGAATGAGA CAGAGAGTTT CCCCAAGGAC TCGGAAATTG CCACGACTTC ACTATCAAAC 
CATTCCTCAT CCTTGGAGGC AGAGTTTCAA GGAACCAAGG AGGTCACCAG GAAGCCAGGG 
GCTGGAGAAT CCATTGAGCC ATCCACAGAG AATGGATTTG GCCCCATATC TTCAGGGGTC 
TCAGTGAGAT TTTGGCAGAA TGATTCCCCA ACCTCGGGAG GGGACCAGCC GGCCAGCAGC 
TCGGAGGCGG GGAGCGGAAG GCCCGCGGCC CGCACCGAGG CCGTGTCTCA GTCGGACCCG 
TTGGGCAGAA GCGGAGACAG CGCGGCGCGC CGGGCCTTGA CCAGCAGCCA CACCGCCGAG 
GCCGTGAGCG GCGGCTCCAC TTCCCAGCCC GCCGCCCCCC TCTCCTCCTC CGGCCCCCGC 
TCTAGGGGAG GCGACCCGGC GCTGGGCACC AGGAGCCACG CTGGGCCGGC CCCCGAGTCT 
TTGTCGTCCT CCTCCTCAGA GAGCCTGGCT GCCTCGGCGC CGCGTGACGA GCGCTCCACC 
ACCGAGGACG GTGCAGAGCC CGGAGGCAGT TCGGACGTGG AGCAGAGGAC GGCGCAGGCC 
CACCCCCCGC ACACGGCTGC CACCTTCACC CGGAACGGGG AGCGCACGCT GCGGTCTCTC 
GCCAACGCCA GCACGACTTC TGGGGACGTG GGGCGCTCTG CCGTCGTGCC CGGGGAGACC 
GACGGCGCCA CCCCGCAAGG GGACGTGCCC GTGACTGGTG ACGCCCGCCT GGTCTCGGGT 
TCACGGGCGG CCTCGCCAGC CCCGGGAGTC ACCGGAGTCA GCGACAGTGG AGTGAGCGGC 
ACGGACACTG AACAGAGGAC TTCCGGTGAC CACACAGACC ACACCTATGT CTCATCCACT 
TTCACCAAAG GAGAACGGGT GTTGCTTTCC ATTATAGACA ACAGCTCATC GGCGGACTTA 
CCGGAGAGTT CCACCTCTGC TGCTAGGATC TCCAACTCTT CACACTCAGA CCATTCCTCC 
TCTTCTCAGG CTCAGACTGA AAGAAGTAAC GTGTCATCCT ATGAAAGGGA GCGTGCTCAG 
CCCTCCACGG AGTCGCTGGC TCTGAGAACA GCCAACGTCC CATCCTCCAC ACCCACCGTT 
AACATGCCAA ACACTTTGGT TCTTCTGGAC ACTGAGGCCA GATCGATGGA TGATGACTCT 
TCTTCTTCTT CTGTTTCTTC TTCTTCAGTT TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT 
TCTTCTTCTT CTGTTTCTTC TTCAGTTTCT TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT 
TCTTCTTCTT CTTCTGTTCC TTCTTCTTCT TCTGTTTCTT CTTCTGTTTC TTCTTCTTCT 
TCTTCTTCTT CTTCTTCTTC TTCAGGACCC CTTTCACCCC TGGCCTCAGT GTCCCAGGCC 
CATCAATTGT TGTCATCAGT CTTACCATCA ATCGGGGCCT CCACCCTTCC ACTGCAGTCC 
ACTCCCGAGG CGCCCACACC TATGTCTTCC TCGCCACCAC CCTTACCAAT ATCCCTGACA 
GCGTCTACCC CTGCGTCACC ATCTGCCTCC CAAACAACCC TCCCACCTTC ACCTTCTACC 
CTGGTCCTCC CCAGAACAAG AGACACTCCT GTGACTTCGG TTCAGATGGA GACAGTGGCA 
TTATCTGTGG CAATGCTCCC CAGTACTCAA ACGGCAGATC CTAAGAACCA GAGTAACCCA 
TACCATGACA AAGTCATTAC AGAGTCAAAG CCACCAAGCC TGGCATCTCT GCCCACAGAG 
GCCACCGGAG CTGTAACAAC GAGGTCTACT TCAGGGTTCC CTACGCCCTC GACCTTAACA 
GATTCCTCCA CTCTGCAAAC CCTTGCAGCC ACGAGCACCA GCATCACCCC GACATCTCCA 
GCATTGACAA CCACCGTTCT TGAGACCTCT CGTCCCTTCA GCCCTGCGTC AACCACAGCA 
GCTCTGATGG CTCACCCCAC AACAGTACAG ACCACGGCTG AAAAACAGCC CTTGACAACC 
AGCCCTGAAA TTCTGGCACC ACGGAGCTCA ACAGAAAGTG CCATCACCAC AGAAAGGAGC 
CAGATCCATG TCGATGGTAC CAGCCCGTTG ACCCCCCTGA CCAGCACACC CACATCACTG 
AAAGGATTGA CCCCAGGGCC TGGTATGACC AAAGAGTACA GCCCGGCTTC ACATTTCCTC 
AGAACATCTC CCCAAACCAC AGGCGTTTCC ACGCTCGAAG TACTGCCTCC TGAATCTACC 
ACCTTTGCTG CTCAGAGCAG CACGCAGCCA CCAAAAGCAT TGTCATCTCC AGCCTCAGTT 
AACAGCTGCG CCACTAACCC TTGTCTTCAT GATGGGAAAT GCATTGTGGA CTCCACCAGC 
CATGGGTATC GATGCGAATG CTCACCTTCC TGGCAAGGGG ATGATTGCAG TGTGGATGTG 
AATGAATGCC TCTTGAGCCC CTGCCCACCC CTGGCCACGT GCAATAATAC TCAGGGATCC 
TTCACCTGCA AATGCCCAGT TGGGTACCAG CTGGAAAAAG GAATATGCAA TTTGGTTAGA 
ACCTTCGTGA CAGAGTTCAA ATTAAAGAAA ACATTTCTCA ATACCACCAT GGAGAAACAC 
GCAGACCTAC ATGAAGTTGA AAATGAGATC ACCAAAACAT TAAATATGTG TTTTTCAACG 
TTACCTGGTT ACACCCGATC CACAGTTCAT GCTTCTAGGG AGTCCAATGT GGTGGTAATG 
TCCTTGAAAA CCACTTTTTC TTTGGCTTCA AAGGTGACAC TGTTTGCCTT GGCCGAGGGG 
ATGCAGAAAT GTGTCAATTC CTGTAGTTCT TCTGCGGAGG CTTGCAAATT CTTGGGATTT 
CAGAGGGGGA TCTTTAAAGC GGGCAGCCTG TGCAAGCGGA AGACTCCAGA ATGTGACAGA 
GAGACCTCCA TCTGCACAGA CCTGGACGGC GTCGCCCTGT GCCAATGCAA GTCGGGCTAC 
TTCCAGTTCA ACAAGATGGA TCACTCCTGC CGAGCATGCG AAGATGGATA TAGGCTTGAA 
AATGAAACCT GTATGAGTTG CCCATTTGGG CTTGGTGGTC TCAACTGTGG AAACCCCTAT 
CAGCTGATCA CCGTAGTGAT CGCAGCAGCA GGAGGAGGGC TTCTACTTAT TCTGGGCATC 
GCACTGATTG TCACCTGCTG CCGAAAGAGT AAAAATGACA TAAGCAAACT CATCTTCAAA 
AGTGGGGATT TCCAAATGTC CCCATATGCT GAGTACCCCA AAAACCCTCG CTCACAAGAA 
TGGGGCCGAG AAGCCATTGA AATGCATGAG AACGGAAGCA CCAAAAACCT CCTCCAGATG 
ACGGATGTGT ATTACTCGCC CACAAGTGTA AGGAATCCTG AACTTGAACG AAATGGACTG 
TACCCGGCCT ACACTGGACT GCCGGGGTCC CGGCACTCGT GCATTTTCCC CGGACAGTAT 
AACCCGTCTT TCATCAGTGA TGAGAGCAGG AGAAGAGACT ACTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022269716.1
CDS25..4272
Translation

Target ORF information:

RefSeq Version XM_022414008.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris heart development protein with EGF like domains 1 (HEG1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022414008.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAACAGTT GGCCAGAAAG TAACACTGAA CCACACGTAG AAAACGGCAC CTTTGATCAG 
AATCAAGTGG ACTTTTCAAC AGTGGTTTCC AAGGAGGGTG TGATGACTCA GACCTCCAGA 
AAGAGCCACA CTTCTTCAAA TGCTCCAGAA AACCTCACTC CACAAACAGA AGCGGCAGAT 
GCTAGAGGAA GAAACGACAC TTCCAGGAAA ACAAATTTCA CTGTTTCCCC TGTTGGGCCT 
GACGTAGCAA CAGCTCTGAC TTCCCAGAGT ATCACCACGG CTTTGGGAAT GCTTCACTTG 
CCATCCAGCT CAGGGTCCGA AGGAAGAACT GCCCCTTCTC ACACGGAGAG CACAACATCC 
CGGGGTTCCC TGGCGAGACG AAAGGGGCTC CCAGACGAAG TGACTGTGCA CACCCAAGTG 
GCTGCCACTT GGGTCTTAGG CCAGTTCCCT CTTCCTGCTT TGGAGACTGG AGAAGAGACC 
ACACTCTCCA GGAAGAGGAA CACCTCAGGA CTGGCGCTCT CCTGGCCACA TTTCTCCAGG 
ACACCAGCTT ACTCCTCTCC CTTGGAGCCC TCCTCGGCTA CTAGGAGCAC AGAGGAGCTC 
AACGACTCCA CTGTTCTCCC GAGTGCTTTG GTGAGTTGGA CAGAGAGTGT GCGTGTTGCT 
GCCACGTTCC CCAGCGGTGT CCCCGGGAGG CTCCAGTCTG TAAGCGTCAG TCTGCAACCT 
CTGAATGAGA CAGAGAGTTT CCCCAAGGAC TCGGAAATTG CCACGACTTC ACTATCAAAC 
CATTCCTCAT CCTTGGAGGC AGAGTTTCAA GGAACCAAGG AGGTCACCAG GAAGCCAGGG 
GCTGGAGAAT CCATTGAGCC ATCCACAGAG AATGGATTTG GCCCCATATC TTCAGGGGTC 
TCAGTGAGAT TTTGGCAGAA TGATTCCCCA ACCTCGGGAG GGGACCAGCC GGCCAGCAGC 
TCGGAGGCGG GGAGCGGAAG GCCCGCGGCC CGCACCGAGG CCGTGTCTCA GTCGGACCCG 
TTGGGCAGAA GCGGAGACAG CGCGGCGCGC CGGGCCTTGA CCAGCAGCCA CACCGCCGAG 
GCCGTGAGCG GCGGCTCCAC TTCCCAGCCC GCCGCCCCCC TCTCCTCCTC CGGCCCCCGC 
TCTAGGGGAG GCGACCCGGC GCTGGGCACC AGGAGCCACG CTGGGCCGGC CCCCGAGTCT 
TTGTCGTCCT CCTCCTCAGA GAGCCTGGCT GCCTCGGCGC CGCGTGACGA GCGCTCCACC 
ACCGAGGACG GTGCAGAGCC CGGAGGCAGT TCGGACGTGG AGCAGAGGAC GGCGCAGGCC 
CACCCCCCGC ACACGGCTGC CACCTTCACC CGGAACGGGG AGCGCACGCT GCGGTCTCTC 
GCCAACGCCA GCACGACTTC TGGGGACGTG GGGCGCTCTG CCGTCGTGCC CGGGGAGACC 
GACGGCGCCA CCCCGCAAGG GGACGTGCCC GTGACTGGTG ACGCCCGCCT GGTCTCGGGT 
TCACGGGCGG CCTCGCCAGC CCCGGGAGTC ACCGGAGTCA GCGACAGTGG AGTGAGCGGC 
ACGGACACTG AACAGAGGAC TTCCGGTGAC CACACAGACC ACACCTATGT CTCATCCACT 
TTCACCAAAG GAGAACGGGT GTTGCTTTCC ATTATAGACA ACAGCTCATC GGCGGACTTA 
CCGGAGAGTT CCACCTCTGC TGCTAGGATC TCCAACTCTT CACACTCAGA CCATTCCTCC 
TCTTCTCAGG CTCAGACTGA AAGAAGTAAC GTGTCATCCT ATGAAAGGGA GCGTGCTCAG 
CCCTCCACGG AGTCGCTGGC TCTGAGAACA GCCAACGTCC CATCCTCCAC ACCCACCGTT 
AACATGCCAA ACACTTTGGT TCTTCTGGAC ACTGAGGCCA GATCGATGGA TGATGACTCT 
TCTTCTTCTT CTGTTTCTTC TTCTTCAGTT TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT 
TCTTCTTCTT CTGTTTCTTC TTCAGTTTCT TCTTCTTCTT CTTCTTCTTC TTCTTCTTCT 
TCTTCTTCTT CTTCTGTTCC TTCTTCTTCT TCTGTTTCTT CTTCTGTTTC TTCTTCTTCT 
TCTTCTTCTT CTTCTTCTTC TTCAGGACCC CTTTCACCCC TGGCCTCAGT GTCCCAGGCC 
CATCAATTGT TGTCATCAGT CTTACCATCA ATCGGGGCCT CCACCCTTCC ACTGCAGTCC 
ACTCCCGAGG CGCCCACACC TATGTCTTCC TCGCCACCAC CCTTACCAAT ATCCCTGACA 
GCGTCTACCC CTGCGTCACC ATCTGCCTCC CAAACAACCC TCCCACCTTC ACCTTCTACC 
CTGGTCCTCC CCAGAACAAG AGACACTCCT GTGACTTCGG TTCAGATGGA GACAGTGGCA 
TTATCTGTGG CAATGCTCCC CAGTACTCAA ACGGCAGATC CTAAGAACCA GAGTAACCCA 
TACCATGACA AAGTCATTAC AGAGTCAAAG CCACCAAGCC TGGCATCTCT GCCCACAGAG 
GCCACCGGAG CTGTAACAAC GAGGTCTACT TCAGGGTTCC CTACGCCCTC GACCTTAACA 
GATTCCTCCA CTCTGCAAAC CCTTGCAGCC ACGAGCACCA GCATCACCCC GACATCTCCA 
GCATTGACAA CCACCGTTCT TGAGACCTCT CGTCCCTTCA GCCCTGCGTC AACCACAGCA 
GCTCTGATGG CTCACCCCAC AACAGTACAG ACCACGGCTG AAAAACAGCC CTTGACAACC 
AGCCCTGAAA TTCTGGCACC ACGGAGCTCA ACAGAAAGTG CCATCACCAC AGAAAGGAGC 
CAGATCCATG TCGATGGTAC CAGCCCGTTG ACCCCCCTGA CCAGCACACC CACATCACTG 
AAAGGATTGA CCCCAGGGCC TGGTATGACC AAAGAGTACA GCCCGGCTTC ACATTTCCTC 
AGAACATCTC CCCAAACCAC AGGCGTTTCC ACGCTCGAAG TACTGCCTCC TGAATCTACC 
ACCTTTGCTG CTCAGAGCAG CACGCAGCCA CCAAAAGCAT TGTCATCTCC AGCCTCAGTT 
AACAGCTGCG CCACTAACCC TTGTCTTCAT GATGGGAAAT GCATTGTGGA CTCCACCAGC 
CATGGGTATC GATGCGAATG CTCACCTTCC TGGCAAGGGG ATGATTGCAG TGTGGATGTG 
AATGAATGCC TCTTGAGCCC CTGCCCACCC CTGGCCACGT GCAATAATAC TCAGGGATCC 
TTCACCTGCA AATGCCCAGT TGGGTACCAG CTGGAAAAAG GAATATGCAA TTTGGTTAGA 
ACCTTCGTGA CAGAGTTCAA ATTAAAGAAA ACATTTCTCA ATACCACCAT GGAGAAACAC 
GCAGACCTAC ATGAAGTTGA AAATGAGATC ACCAAAACAT TAAATATGTG TTTTTCAACG 
TTACCTGGTT ACACCCGATC CACAGTTCAT GCTTCTAGGG AGTCCAATGT GGTGGTAATG 
TCCTTGAAAA CCACTTTTTC TTTGGCTTCA AAGGTGACAC TGTTTGCCTT GGCCGAGGGG 
ATGCAGAAAT GTGTCAATTC CTGTAGTTCT TCTGCGGAGG CTTGCAAATT CTTGGGATTT 
CAGAGGGGGA TCTTTAAAGC GGGCAGCCTG TGCAAGCGGA AGACTCCAGA ATGTGACAGA 
GAGACCTCCA TCTGCACAGA CCTGGACGGC GTCGCCCTGT GCCAATGCAA GTCGGGCTAC 
TTCCAGTTCA ACAAGATGGA TCACTCCTGC CGAGCATGCG AAGATGGATA TAGGCTTGAA 
AATGAAACCT GTATGAGTTG CCCATTTGGG CTTGGTGGTC TCAACTGTGG AAACCCCTAT 
CAGCTGATCA CCGTAGTGAT CGCAGCAGCA GGAGGAGGGC TTCTACTTAT TCTGGGCATC 
GCACTGATTG TCACCTGCTG CCGAAAGAGT AAAAATGACA TAAGCAAACT CATCTTCAAA 
AGTGGGGATT TCCAAATGTC CCCATATGCT GAGTACCCCA AAAACCCTCG CTCACAAGAA 
TGGGGCCGAG AAGCCATTGA AATGCATGAG AACGGAAGCA CCAAAAACCT CCTCCAGATG 
ACGGATGTGT ATTACTCGCC CACAAGTGTA AGGAATCCTG AACTTGAACG AAATGGACTG 
TACCCGGCCT ACACTGGACT GCCGGGGTCC CGGCACTCGT GCATTTTCCC CGGACAGTAT 
AACCCGTCTT TCATCAGTGA TGAGAGCAGG AGAAGAGACT ACTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed