• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

RASGRF2 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol RASGRF2
Entrez Gene ID 610203
Full Name Ras protein specific guanine nucleotide releasing factor 2
General protein information
Preferred Names

Ras protein specific guanine nucleotide releasing factor 2

Names

ras-specific guanine nucleotide-releasing factor 2
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_014111758.2 XP_013967233.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_014111758.1 XP_013967233.1 ras-specific guanine nucleotide-releasing factor 2
XM_022416755.1 XP_022272463.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) RASGRF2 XP_005618219.1
Danio rerio (zebrafish) rasgrf2b NP_001121705.1
Pan troglodytes (chimpanzee) RASGRF2 XP_517672.2
Xenopus tropicalis (tropical clawed frog) rasgrf2 XP_002942408.2
Homo sapiens (human) RASGRF2 NP_008840.1
Mus musculus (house mouse) Rasgrf2 NP_033053.2
Rattus norvegicus (Norway rat) Rasgrf2 NP_446173.1
Gallus gallus (chicken) RASGRF2 XP_004949517.1
Bos taurus (cattle) RASGRF2 XP_002689419.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001238 XP_321917.5

Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287134 XM_022416755.1
Latest version!		 Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OCe35549 XM_014111758.2
Latest version!		 Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OCe35549 XM_014111758.1 Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe287134
Clone ID Related Accession (Same CDS sequence) XM_022416755.1
Accession Version XM_022416755.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGGATT TAACAACACT GAGAGGACAT GTGACAAAGA GTTTATTATT 
CGAAGAACAG CCACCAATCG AGTCCTGAAT GTCCTCCGTC ACTGGGTCTC AAAGCACGCA 
CAGGATTTTG AACTCAACAA CGAACTAAAG ATGAATGTCC TAAATTTGCT AGAAGAAGTT 
TTACGAGACC CAGACCTTCT TCCCCAGGAA AGGAAAGCCA CGGCGAATAT CCTGAGGGCC 
CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAATTAG AGGATATAAT TCAACTGACA 
GACTGTCCGA AGGCTGAGTG CTTTGAGACC TTGTCAGCCA TGGAGCTGGC TGAGCAGATC 
ACCCTCCTGG ACCACATCAT CTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG 
TGGATGAAGC TGGATAAAAA TGAAAGAACA CCTTACATTA TGAAAACCAG CCAACACTTC 
AATGATATGA GTAACCTGGT GGCCTCCCAG ATAATGAACT ATGCGGACGT CAGCTCGAGG 
GCCAACGCGA TAGAGAAGTG GGTGGCCGTG GCGGACATCT GCCGATGCCT GCACAACTAC 
AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAGA 
ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACCGTT 
TCATCTGAAG GAAGATTTAA AAATCTCAGA GAAACCCTTA AAAACTGTAA CCCCCCAGCT 
GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCCTTTA TTGAAGAAGG AACACCAAAC 
TTTACTGAGG AAGGCCTCGT TAATTTCTCC AAAATGAGAA TGATATCACA CATCATCAGA 
GAGATACGCC AATTCCAGCA GACTTGCTAT CGAATAGACC ATCAGCCAAA GGGAGAGCTC 
TGCTTTACAA GAAGAAACAG CAAAATACAG AGTTCTTGGT ACCTGAGCCT ATTGCACCTG 
CTGGTTTCTC TTGGAGGCAT GAAGTATATT GATGCTGACA GTGAAAAAAA AAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022272463.1
CDS101..3637
Translation

Target ORF information:

RefSeq Version XM_022416755.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022416755.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGGATT TAACAACACT GAGAGGACAT GTGACAAAGA GTTTATTATT 
CGAAGAACAG CCACCAATCG AGTCCTGAAT GTCCTCCGTC ACTGGGTCTC AAAGCACGCA 
CAGGATTTTG AACTCAACAA CGAACTAAAG ATGAATGTCC TAAATTTGCT AGAAGAAGTT 
TTACGAGACC CAGACCTTCT TCCCCAGGAA AGGAAAGCCA CGGCGAATAT CCTGAGGGCC 
CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAATTAG AGGATATAAT TCAACTGACA 
GACTGTCCGA AGGCTGAGTG CTTTGAGACC TTGTCAGCCA TGGAGCTGGC TGAGCAGATC 
ACCCTCCTGG ACCACATCAT CTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG 
TGGATGAAGC TGGATAAAAA TGAAAGAACA CCTTACATTA TGAAAACCAG CCAACACTTC 
AATGATATGA GTAACCTGGT GGCCTCCCAG ATAATGAACT ATGCGGACGT CAGCTCGAGG 
GCCAACGCGA TAGAGAAGTG GGTGGCCGTG GCGGACATCT GCCGATGCCT GCACAACTAC 
AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAGA 
ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACCGTT 
TCATCTGAAG GAAGATTTAA AAATCTCAGA GAAACCCTTA AAAACTGTAA CCCCCCAGCT 
GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCCTTTA TTGAAGAAGG AACACCAAAC 
TTTACTGAGG AAGGCCTCGT TAATTTCTCC AAAATGAGAA TGATATCACA CATCATCAGA 
GAGATACGCC AATTCCAGCA GACTTGCTAT CGAATAGACC ATCAGCCAAA GGGAGAGCTC 
TGCTTTACAA GAAGAAACAG CAAAATACAG AGTTCTTGGT ACCTGAGCCT ATTGCACCTG 
CTGGTTTCTC TTGGAGGCAT GAAGTATATT GATGCTGACA GTGAAAAAAA AAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe35549
Clone ID Related Accession (Same CDS sequence) XM_014111758.2 , XM_014111758.1
Accession Version XM_014111758.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT 
TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT 
CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT 
GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC 
AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC 
CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA 
CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC 
CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT 
GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG 
GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT 
CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT 
TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT 
TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA 
ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG 
TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013967233.1
CDS100..3852
Translation

Target ORF information:

RefSeq Version XM_014111758.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014111758.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT 
TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT 
CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT 
GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC 
AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC 
CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA 
CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC 
CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT 
GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG 
GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT 
CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT 
TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT 
TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA 
ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG 
TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe35549
Clone ID Related Accession (Same CDS sequence) XM_014111758.2 , XM_014111758.1
Accession Version XM_014111758.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product ras-specific guanine nucleotide-releasing factor 2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT 
TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT 
CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT 
GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC 
AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC 
CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA 
CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC 
CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT 
GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG 
GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT 
CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT 
TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT 
TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA 
ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG 
TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013967233.1
CDS1..3753
Translation

Target ORF information:

RefSeq Version XM_014111758.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014111758.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC 
CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC 
GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA 
GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG 
TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG 
AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG 
TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC 
CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT 
CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG 
GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG 
GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT 
CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG 
GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT 
TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG 
AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT 
GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC 
TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT 
TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA 
AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC 
TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG 
ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA 
CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC 
CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT 
TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT 
CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT 
GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC 
AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC 
CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA 
CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC 
CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT 
GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG 
GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT 
CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT 
TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT 
TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA 
ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG 
TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed