hectd1 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa47647 XM_009293116.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa36285 NM_001002504.2
Latest version!
Danio rerio HECT domain containing 1 (hectd1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47642 XM_009293111.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47643 XM_009293112.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47644 XM_009293113.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47645 XM_009293114.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47646 XM_009293115.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47648 XM_009293117.3
Latest version!
Danio rerio HECT domain containing 1 (hectd1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
ODa47642 XM_009293111.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47643 XM_009293112.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47644 XM_009293113.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47648 XM_009293117.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47647 XM_009293116.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa47645 XM_009293114.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa47646 XM_009293115.2 Danio rerio HECT domain containing 1 (hectd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa47647
    Clone ID Related Accession (Same CDS sequence) XM_009293116.3 , XM_009293116.2
    Accession Version XM_009293116.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293116.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGTTTGCTAT TTTGAAGTGC AACCTTTATC CCTCCCCCCC
    AACTTAACAG GTAACATTTT TGATGTACTA CTCATATTCT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291391.1
    CDS463..4467
    Translation

    Target ORF information:

    RefSeq Version XM_009293116.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293116.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGTTTGCTAT TTTGAAGTGC AACCTTTATC CCTCCCCCCC
    AACTTAACAG GTAACATTTT TGATGTACTA CTCATATTCT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47647
    Clone ID Related Accession (Same CDS sequence) XM_009293116.3 , XM_009293116.2
    Accession Version XM_009293116.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGTTTGCTAT TTTGAAGTGC AACCTTTATC CCTCCCCCCC
    AACTTAACAG GTAACATTTT TGATGTACTA CTCATATTCT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291391.1
    CDS463..4467
    Translation

    Target ORF information:

    RefSeq Version XM_009293116.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293116.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGTTTGCTAT TTTGAAGTGC AACCTTTATC CCTCCCCCCC
    AACTTAACAG GTAACATTTT TGATGTACTA CTCATATTCT GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa36285
    Clone ID Related Accession (Same CDS sequence) NM_001002504.2
    Accession Version NM_001002504.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL593845.5. On or before Feb 8, 2008 this sequence version replaced XM_001337775.1, NM_001002504.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505392, SAMEA4476736 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGGACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT GCTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCCACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAT
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AGGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCCACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAACC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGTGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGTGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGACTTCC TGGACAGTTG TCGGGCCAAC
    ACTTTGCTTG CAGAGTTGGA TGATGAAGAA GATTTGCCGG AGCCAGATGA TGACGATGAT
    GAAAATGAGG ATGACAATCA GGAGGAACAG GAGTATGAGG AAGTTTTGGT ACGGTCCAGG
    GTCAACCTTG GTTACCACGT TCATATTCAT AGGGAGGAAG AGGAGTATGA AACCAAAGGG
    GGCCGTCGCA GGACCTGGGA TGACGACTTT GTGCTGAAAC GACAGTTTTC TGCTTTAGTA
    CCTGCATTTG ACCCTAGACC AGGCAGGACC AACGTCCAGC AAACTACTGA CCTGGAAATC
    CCCCCACCAG GAACACCTCG TTCAGAGGTA CAGGAGGAGG TGGAGTGCGC TCCTTCACCT
    CGTCTGGCTC TCATCTTAAA AGTAGCAGGT CTAGGAACTA CTCGAGAAGT AGAACTACCT
    CTCACCAATT ACAAGTCCAC CATTTTCTAT TATGTCCAAA AACTTCTCCA GCTGTCATGC
    AACGGTGCTA TTAAACCTGA CAAGCTCAGA CGCATCTGGG AGCCTACGTA TACGATAATG
    TATAGAGAAT TGAAGGATTC CGACAAAGAA AGAGAGAGCG GAAAGATGGG TTGCTGGTCT
    GTAGAGCATG TGGAACAGTA CCTTGGCACA GATGAATTAC CAAAGAATGA CTTGATAACC
    TACATGCAGA AGAATGCAGA CTCAACTTTC CTGCGCCACT GGAAATTAAC CGGCTCTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCG CAGCTCATAG CTGCATACAA GGACTTCTGT
    GAGCGTGGCT GCAGATCTTC AGGCCTGAGT TCAGGGACGT TGTCCACGAC GCAAAGCTGT
    GATATCTTGA GTGCTGCAAG AGAGCAGGCT CAGGCTAAAG CGGGCTCGGG TCAGAGCGCC
    TGCAGCGTAG AGGATGTACT GCAGCTCTTG CGCATCCTTT TCACCATCGG AGGAGAACCT
    ACATCTGGAC GCACACTACA GGAAGATGTG GAAGAGCTAC AGTTCAATGC ATCACCTGAG
    GAATTCACCA GCAAAAAAAT TACAACCAAA ATCCTGCAGC AGATTGAAGA GCCATTGGCT
    CTGGCTAGCG GGGCTCTCCC CGACTGGTGT GAACAGTTAA CCAGCAAGTG TCCTTTCCTC
    ATACCCTTTG AAACCCGGCA GCTTTATTTT ACCTGCACTG CATTTGGAGC CTCCAGGGCA
    ATTGTTTGGC TCCAGAATCG AAGGGAAGCC ACTATGGAGC GTTCCCGGCC ATCCACGACT
    GTTCGCCGTG ATGATCCCGG AGAGTTCCGT GTTGGTCGGC TTAAGCATGA ACGTGTCAAG
    GTGCCTCGAG GAGAGAGTAT GATGGAGTGG GCTGAGAGTG TGATGCAGAT ACACGCTGAC
    AGAAAGTCTG TACTGGAGGT GGAGTTCCAA GGGGAGGAGG GCACTGGTCT CGGACCCACC
    CTGGAGTTCT ATGCTCTTGT TGCGGCAGAG TTTCAGAGGA CCTCCCTTGG CATCTGGCTC
    TGTGATGATG ACTTCCCAGA TGATGAGTCA CGTCAGGTGG ATCTGGGCGG TGGCTTGAAA
    CCGCCAGGCT ATTACGTGCA GCGTTCCTGC GGCCTTTTCC CTGCTCCCTT CCCTCAGGAC
    AGCGATGAAC TGGAGCGTAT TACCAAACTT TTCTTGTTCC TTGGCATCTT TCTGGCCAAA
    TGCATCCAGG ACAACCGGCT CGTGGATCTG CCCATATCCC AGCCTTTCTT TAAACTGCTC
    TGTATGGGTG ACATCAAAAG CAACATGAGT AAGCTACTTT ATCAAACACG TGGTGAGTCG
    GACTGTCACT TCTCTGAAAT CCAGTCTGAG GCCTCCACTG AGGAGGGTCA GGACACGTAC
    TCTGTGGGTA GTTTTGATGA AGACTCCAAG TCTGAGTTTA TTCTTGACCC ACCGAAGCCC
    AAACCACCAG CCTGGTATCA TGGCATCTTG ACCTGGGAGG ACTTTGAACT GGTAAACCCT
    CACAGAGCAC TGTTTCTTAA AGAGCTGAAG GCACTTTCTG TGAAGCGCAG GCAGATTCTA
    GGCAATAAGA GTCTGTCCGA GGATGAGAAG AACACACGGC TTCAGGATCT CATGCTGAAG
    AATCCTATGG GCTCTGGCCC TCCACTGTGT GTTGAAGATC TAGGATTAAA TTTCCAGTTC
    TGTCCATCAT CCAAAGTACA TGGTTTCTCT TCTGTAGACT TGAAGCCTAA TGGAGAGGAT
    GAGATGGTCA CCATGGACAA TGCAGAGGAG TATGTAGAGC TGATGTTTGA CTTCTGCATG
    CACACAGGAA TCCAGAAGCA AATGGAGGCC TTTAGAGAGG GCTTTAATAA AGTGTTCCCT
    ATGGAGAAAC TGAGCTCATT TAGCCATAAA GAGGTGCAGA TGATCCTGTG TGGAAACCAG
    TCTCCATCCT GGACTGCTGA AGACATTGTT AACTACACAG AACCTAAACT GGGCTATACA
    CGGGACAGTC CTGGTTTCCT GCGTTTCGTG CGGGTTCTGT GTGGAATGTC TTCTGATGAG
    AGGAAAGCTT TCCTCCAGTT CACTACAGGC TGCTCAACTC TGCCCCCTGG TGGACTGGCC
    AACCTCCATC CACGGCTCAC AATAGTCAGA AAGGTCGATG CAACAGATGC CAGCTATCCT
    TCAGTCAACA CGTGTGTGCA CTACTTGAAG CTGCCGGAAT ATTCTTCAGA AGAGATTATG
    AGAGAGCGCC TTCTCGCTGC CACTATGGAG AAGGGTTTCC ACCTCAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002504.2
    CDS102..7832
    Translation

    Target ORF information:

    RefSeq Version NM_001002504.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGGACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT GCTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCCACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAT
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AGGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCCACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAACC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGTGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGTGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGACTTCC TGGACAGTTG TCGGGCCAAC
    ACTTTGCTTG CAGAGTTGGA TGATGAAGAA GATTTGCCGG AGCCAGATGA TGACGATGAT
    GAAAATGAGG ATGACAATCA GGAGGAACAG GAGTATGAGG AAGTTTTGGT ACGGTCCAGG
    GTCAACCTTG GTTACCACGT TCATATTCAT AGGGAGGAAG AGGAGTATGA AACCAAAGGG
    GGCCGTCGCA GGACCTGGGA TGACGACTTT GTGCTGAAAC GACAGTTTTC TGCTTTAGTA
    CCTGCATTTG ACCCTAGACC AGGCAGGACC AACGTCCAGC AAACTACTGA CCTGGAAATC
    CCCCCACCAG GAACACCTCG TTCAGAGGTA CAGGAGGAGG TGGAGTGCGC TCCTTCACCT
    CGTCTGGCTC TCATCTTAAA AGTAGCAGGT CTAGGAACTA CTCGAGAAGT AGAACTACCT
    CTCACCAATT ACAAGTCCAC CATTTTCTAT TATGTCCAAA AACTTCTCCA GCTGTCATGC
    AACGGTGCTA TTAAACCTGA CAAGCTCAGA CGCATCTGGG AGCCTACGTA TACGATAATG
    TATAGAGAAT TGAAGGATTC CGACAAAGAA AGAGAGAGCG GAAAGATGGG TTGCTGGTCT
    GTAGAGCATG TGGAACAGTA CCTTGGCACA GATGAATTAC CAAAGAATGA CTTGATAACC
    TACATGCAGA AGAATGCAGA CTCAACTTTC CTGCGCCACT GGAAATTAAC CGGCTCTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCG CAGCTCATAG CTGCATACAA GGACTTCTGT
    GAGCGTGGCT GCAGATCTTC AGGCCTGAGT TCAGGGACGT TGTCCACGAC GCAAAGCTGT
    GATATCTTGA GTGCTGCAAG AGAGCAGGCT CAGGCTAAAG CGGGCTCGGG TCAGAGCGCC
    TGCAGCGTAG AGGATGTACT GCAGCTCTTG CGCATCCTTT TCACCATCGG AGGAGAACCT
    ACATCTGGAC GCACACTACA GGAAGATGTG GAAGAGCTAC AGTTCAATGC ATCACCTGAG
    GAATTCACCA GCAAAAAAAT TACAACCAAA ATCCTGCAGC AGATTGAAGA GCCATTGGCT
    CTGGCTAGCG GGGCTCTCCC CGACTGGTGT GAACAGTTAA CCAGCAAGTG TCCTTTCCTC
    ATACCCTTTG AAACCCGGCA GCTTTATTTT ACCTGCACTG CATTTGGAGC CTCCAGGGCA
    ATTGTTTGGC TCCAGAATCG AAGGGAAGCC ACTATGGAGC GTTCCCGGCC ATCCACGACT
    GTTCGCCGTG ATGATCCCGG AGAGTTCCGT GTTGGTCGGC TTAAGCATGA ACGTGTCAAG
    GTGCCTCGAG GAGAGAGTAT GATGGAGTGG GCTGAGAGTG TGATGCAGAT ACACGCTGAC
    AGAAAGTCTG TACTGGAGGT GGAGTTCCAA GGGGAGGAGG GCACTGGTCT CGGACCCACC
    CTGGAGTTCT ATGCTCTTGT TGCGGCAGAG TTTCAGAGGA CCTCCCTTGG CATCTGGCTC
    TGTGATGATG ACTTCCCAGA TGATGAGTCA CGTCAGGTGG ATCTGGGCGG TGGCTTGAAA
    CCGCCAGGCT ATTACGTGCA GCGTTCCTGC GGCCTTTTCC CTGCTCCCTT CCCTCAGGAC
    AGCGATGAAC TGGAGCGTAT TACCAAACTT TTCTTGTTCC TTGGCATCTT TCTGGCCAAA
    TGCATCCAGG ACAACCGGCT CGTGGATCTG CCCATATCCC AGCCTTTCTT TAAACTGCTC
    TGTATGGGTG ACATCAAAAG CAACATGAGT AAGCTACTTT ATCAAACACG TGGTGAGTCG
    GACTGTCACT TCTCTGAAAT CCAGTCTGAG GCCTCCACTG AGGAGGGTCA GGACACGTAC
    TCTGTGGGTA GTTTTGATGA AGACTCCAAG TCTGAGTTTA TTCTTGACCC ACCGAAGCCC
    AAACCACCAG CCTGGTATCA TGGCATCTTG ACCTGGGAGG ACTTTGAACT GGTAAACCCT
    CACAGAGCAC TGTTTCTTAA AGAGCTGAAG GCACTTTCTG TGAAGCGCAG GCAGATTCTA
    GGCAATAAGA GTCTGTCCGA GGATGAGAAG AACACACGGC TTCAGGATCT CATGCTGAAG
    AATCCTATGG GCTCTGGCCC TCCACTGTGT GTTGAAGATC TAGGATTAAA TTTCCAGTTC
    TGTCCATCAT CCAAAGTACA TGGTTTCTCT TCTGTAGACT TGAAGCCTAA TGGAGAGGAT
    GAGATGGTCA CCATGGACAA TGCAGAGGAG TATGTAGAGC TGATGTTTGA CTTCTGCATG
    CACACAGGAA TCCAGAAGCA AATGGAGGCC TTTAGAGAGG GCTTTAATAA AGTGTTCCCT
    ATGGAGAAAC TGAGCTCATT TAGCCATAAA GAGGTGCAGA TGATCCTGTG TGGAAACCAG
    TCTCCATCCT GGACTGCTGA AGACATTGTT AACTACACAG AACCTAAACT GGGCTATACA
    CGGGACAGTC CTGGTTTCCT GCGTTTCGTG CGGGTTCTGT GTGGAATGTC TTCTGATGAG
    AGGAAAGCTT TCCTCCAGTT CACTACAGGC TGCTCAACTC TGCCCCCTGG TGGACTGGCC
    AACCTCCATC CACGGCTCAC AATAGTCAGA AAGGTCGATG CAACAGATGC CAGCTATCCT
    TCAGTCAACA CGTGTGTGCA CTACTTGAAG CTGCCGGAAT ATTCTTCAGA AGAGATTATG
    AGAGAGCGCC TTCTCGCTGC CACTATGGAG AAGGGTTTCC ACCTCAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47642
    Clone ID Related Accession (Same CDS sequence) XM_009293111.3 , XM_009293111.2
    Accession Version XM_009293111.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGGTTG CTGGTCTGTA GAGCATGTGG AACAGTACCT TGGCACAGAT
    GAATTACCAA AGAATGACTT GATAACCTAC ATGCAGAAGA ATGCAGACTC AACTTTCCTG
    CGCCACTGGA AATTAACCGG CTCTAATAAA AGTATTAGGA AAAACAGAAA TTGTTCGCAG
    CTCATAGCTG CATACAAGGA CTTCTGTGAG CGTGGCTGCA GATCTTCAGG CCTGAGTTCA
    GGGACGTTGT CCACGACGCA AAGCTGTGAT ATCTTGAGTG CTGCAAGAGA GCAGGCTCAG
    GCTAAAGCGG GCTCGGGTCA GAGCGCCTGC AGCGTAGAGG ATGTACTGCA GCTCTTGCGC
    ATCCTTTTCA CCATCGGAGG AGAACCTACA TCTGGACGCA CACTACAGGA AGATGTGGAA
    GAGCTACAGT TCAATGCATC ACCTGAGGAA TTCACCAGCA AAAAAATTAC AACCAAAATC
    CTGCAGCAGA TTGAAGAGCC ATTGGCTCTG GCTAGTGGGG CTCTCCCCGA CTGGTGTGAA
    CAGTTAACCA GCAAGTGTCC TTTCCTCATA CCCTTTGAAA CCCGGCAGCT TTATTTTACC
    TGCACTGCAT TTGGAGCCTC TAGGGCAATT GTTTGGCTCC AGAATCGAAG GGAAGCCACT
    ATGGAGCGTT CCCGGCCATC CACGACTGTT CGCCGTGATG ATCCCGGAGA GTTCCGTGTT
    GGTCGGCTTA AGCATGAACG TGTCAAGGTG CCTCGAGGAG AGAGTATGAT GGAGTGGGCT
    GAGAGTGTGA TGCAGATACA CGCTGACAGA AAGTCTGTAC TGGAGGTGGA GTTCCAAGGG
    GAGGAGGGCA CTGGTCTCGG ACCCACCCTG GAGTTCTATG CTCTTGTTGC GGCAGAGTTT
    CAGAGGACCT CCCTTGGCAT CTGGCTCTGT GATGATGACT TCCCAGATGA TGAGTCACGT
    CAGGTGGATC TGGGCGGTGG CTTGAAACCG CCAGGCTATT ACGTGCAGCG TTCCTGCGGC
    CTTTTCCCTG CTCCCTTCCC TCAGGACAGC GATGAACTGG AGCGTATTAC CAAACTTTTC
    TTGTTCCTTG GCATCTTTCT GGCCAAATGC ATCCAGGACA ACCGGCTCGT GGATCTGCCC
    ATATCCCAGC CTTTCTTTAA ACTGCTCTGT ATGGGTGACA TCAAAAGCAA CATGAGTAAG
    CTACTTTATC AAACCCGTGG TGAGTCGGAC TGTCACTTCT CTGAAATCCA GTCTGAGGCC
    TCCACCGAGG AGGGTCAGGA CACGTACTCT GTGGGTAGTT TTGATGAAGA CTCCAAGTCT
    GAGTTTATTC TTGACCCACC GAAGCCCAAA CCACCAGCCT GGTATCATGG CATCTTGACC
    TGGGAGGACT TTGAACTGGT AAACCCTCAC AGAGCACTGT TTCTTAAAGA GCTGAAGGCA
    CTTTCTGTGA AGCGCAGGCA GATTCTAGGC AATAAGAGTC TGTCCGAGGA TGAGAAGAAC
    ACACGGCTTC AGGATCTCAT GCTGAAGAAT CCTATGGGCT CTGGCCCTCC ACTGTGTGTT
    GAAGATCTAG GATTAAATTT CCAGTTCTGT CCATCATCCA AAGTACATGG TTTCTCTTCT
    GTAGACTTGA AGCCTAATGG AGAGGATGAG ATGGTCACCA TGGACAATGC AGAGGAGTAT
    GTAGAGCTGA TGTTTGACTT CTGCATGCAC ACAGGAATCC AGAAGCAAAT GGAGGCCTTT
    AGAGAGGGCT TTAATAAAGT GTTCCCTATG GAGAAACTGA GCTCATTTAG CCATAAAGAG
    GTGCAGATGA TCCTGTGTGG AAACCAGTCT CCATCCTGGA CTGCTGAAGA CATTGTTAAC
    TACACAGAAC CTAAACTGGG CTATACACGG GACAGTCCTG GTTTCCTGCG TTTCGTGCGG
    GTTCTGTGTG GAATGTCTTC TGATGAGAGG AAAGCTTTCC TCCAGTTCAC TACAGGCTGC
    TCAACTCTGC CCCCTGGTGG ACTGGCCAAC CTCCATCCAC GGCTCACAAT AGTCCGAAAG
    GTTGATGCAA CAGATGCCAG CTATCCTTCA GTCAACACGT GTGTGCACTA CTTGAAGCTG
    CCGGAATATT CTTCAGAAGA GATTATGAGA GAGCGCCTTC TCGCTGCCAC TATGGAGAAG
    GGTTTCCACC TCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291386.1
    CDS463..8220
    Translation

    Target ORF information:

    RefSeq Version XM_009293111.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293111.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGGTTG CTGGTCTGTA GAGCATGTGG AACAGTACCT TGGCACAGAT
    GAATTACCAA AGAATGACTT GATAACCTAC ATGCAGAAGA ATGCAGACTC AACTTTCCTG
    CGCCACTGGA AATTAACCGG CTCTAATAAA AGTATTAGGA AAAACAGAAA TTGTTCGCAG
    CTCATAGCTG CATACAAGGA CTTCTGTGAG CGTGGCTGCA GATCTTCAGG CCTGAGTTCA
    GGGACGTTGT CCACGACGCA AAGCTGTGAT ATCTTGAGTG CTGCAAGAGA GCAGGCTCAG
    GCTAAAGCGG GCTCGGGTCA GAGCGCCTGC AGCGTAGAGG ATGTACTGCA GCTCTTGCGC
    ATCCTTTTCA CCATCGGAGG AGAACCTACA TCTGGACGCA CACTACAGGA AGATGTGGAA
    GAGCTACAGT TCAATGCATC ACCTGAGGAA TTCACCAGCA AAAAAATTAC AACCAAAATC
    CTGCAGCAGA TTGAAGAGCC ATTGGCTCTG GCTAGTGGGG CTCTCCCCGA CTGGTGTGAA
    CAGTTAACCA GCAAGTGTCC TTTCCTCATA CCCTTTGAAA CCCGGCAGCT TTATTTTACC
    TGCACTGCAT TTGGAGCCTC TAGGGCAATT GTTTGGCTCC AGAATCGAAG GGAAGCCACT
    ATGGAGCGTT CCCGGCCATC CACGACTGTT CGCCGTGATG ATCCCGGAGA GTTCCGTGTT
    GGTCGGCTTA AGCATGAACG TGTCAAGGTG CCTCGAGGAG AGAGTATGAT GGAGTGGGCT
    GAGAGTGTGA TGCAGATACA CGCTGACAGA AAGTCTGTAC TGGAGGTGGA GTTCCAAGGG
    GAGGAGGGCA CTGGTCTCGG ACCCACCCTG GAGTTCTATG CTCTTGTTGC GGCAGAGTTT
    CAGAGGACCT CCCTTGGCAT CTGGCTCTGT GATGATGACT TCCCAGATGA TGAGTCACGT
    CAGGTGGATC TGGGCGGTGG CTTGAAACCG CCAGGCTATT ACGTGCAGCG TTCCTGCGGC
    CTTTTCCCTG CTCCCTTCCC TCAGGACAGC GATGAACTGG AGCGTATTAC CAAACTTTTC
    TTGTTCCTTG GCATCTTTCT GGCCAAATGC ATCCAGGACA ACCGGCTCGT GGATCTGCCC
    ATATCCCAGC CTTTCTTTAA ACTGCTCTGT ATGGGTGACA TCAAAAGCAA CATGAGTAAG
    CTACTTTATC AAACCCGTGG TGAGTCGGAC TGTCACTTCT CTGAAATCCA GTCTGAGGCC
    TCCACCGAGG AGGGTCAGGA CACGTACTCT GTGGGTAGTT TTGATGAAGA CTCCAAGTCT
    GAGTTTATTC TTGACCCACC GAAGCCCAAA CCACCAGCCT GGTATCATGG CATCTTGACC
    TGGGAGGACT TTGAACTGGT AAACCCTCAC AGAGCACTGT TTCTTAAAGA GCTGAAGGCA
    CTTTCTGTGA AGCGCAGGCA GATTCTAGGC AATAAGAGTC TGTCCGAGGA TGAGAAGAAC
    ACACGGCTTC AGGATCTCAT GCTGAAGAAT CCTATGGGCT CTGGCCCTCC ACTGTGTGTT
    GAAGATCTAG GATTAAATTT CCAGTTCTGT CCATCATCCA AAGTACATGG TTTCTCTTCT
    GTAGACTTGA AGCCTAATGG AGAGGATGAG ATGGTCACCA TGGACAATGC AGAGGAGTAT
    GTAGAGCTGA TGTTTGACTT CTGCATGCAC ACAGGAATCC AGAAGCAAAT GGAGGCCTTT
    AGAGAGGGCT TTAATAAAGT GTTCCCTATG GAGAAACTGA GCTCATTTAG CCATAAAGAG
    GTGCAGATGA TCCTGTGTGG AAACCAGTCT CCATCCTGGA CTGCTGAAGA CATTGTTAAC
    TACACAGAAC CTAAACTGGG CTATACACGG GACAGTCCTG GTTTCCTGCG TTTCGTGCGG
    GTTCTGTGTG GAATGTCTTC TGATGAGAGG AAAGCTTTCC TCCAGTTCAC TACAGGCTGC
    TCAACTCTGC CCCCTGGTGG ACTGGCCAAC CTCCATCCAC GGCTCACAAT AGTCCGAAAG
    GTTGATGCAA CAGATGCCAG CTATCCTTCA GTCAACACGT GTGTGCACTA CTTGAAGCTG
    CCGGAATATT CTTCAGAAGA GATTATGAGA GAGCGCCTTC TCGCTGCCAC TATGGAGAAG
    GGTTTCCACC TCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47642
    Clone ID Related Accession (Same CDS sequence) XM_009293111.3 , XM_009293111.2
    Accession Version XM_009293111.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGGTTG CTGGTCTGTA GAGCATGTGG AACAGTACCT TGGCACAGAT
    GAATTACCAA AGAATGACTT GATAACCTAC ATGCAGAAGA ATGCAGACTC AACTTTCCTG
    CGCCACTGGA AATTAACCGG CTCTAATAAA AGTATTAGGA AAAACAGAAA TTGTTCGCAG
    CTCATAGCTG CATACAAGGA CTTCTGTGAG CGTGGCTGCA GATCTTCAGG CCTGAGTTCA
    GGGACGTTGT CCACGACGCA AAGCTGTGAT ATCTTGAGTG CTGCAAGAGA GCAGGCTCAG
    GCTAAAGCGG GCTCGGGTCA GAGCGCCTGC AGCGTAGAGG ATGTACTGCA GCTCTTGCGC
    ATCCTTTTCA CCATCGGAGG AGAACCTACA TCTGGACGCA CACTACAGGA AGATGTGGAA
    GAGCTACAGT TCAATGCATC ACCTGAGGAA TTCACCAGCA AAAAAATTAC AACCAAAATC
    CTGCAGCAGA TTGAAGAGCC ATTGGCTCTG GCTAGTGGGG CTCTCCCCGA CTGGTGTGAA
    CAGTTAACCA GCAAGTGTCC TTTCCTCATA CCCTTTGAAA CCCGGCAGCT TTATTTTACC
    TGCACTGCAT TTGGAGCCTC TAGGGCAATT GTTTGGCTCC AGAATCGAAG GGAAGCCACT
    ATGGAGCGTT CCCGGCCATC CACGACTGTT CGCCGTGATG ATCCCGGAGA GTTCCGTGTT
    GGTCGGCTTA AGCATGAACG TGTCAAGGTG CCTCGAGGAG AGAGTATGAT GGAGTGGGCT
    GAGAGTGTGA TGCAGATACA CGCTGACAGA AAGTCTGTAC TGGAGGTGGA GTTCCAAGGG
    GAGGAGGGCA CTGGTCTCGG ACCCACCCTG GAGTTCTATG CTCTTGTTGC GGCAGAGTTT
    CAGAGGACCT CCCTTGGCAT CTGGCTCTGT GATGATGACT TCCCAGATGA TGAGTCACGT
    CAGGTGGATC TGGGCGGTGG CTTGAAACCG CCAGGCTATT ACGTGCAGCG TTCCTGCGGC
    CTTTTCCCTG CTCCCTTCCC TCAGGACAGC GATGAACTGG AGCGTATTAC CAAACTTTTC
    TTGTTCCTTG GCATCTTTCT GGCCAAATGC ATCCAGGACA ACCGGCTCGT GGATCTGCCC
    ATATCCCAGC CTTTCTTTAA ACTGCTCTGT ATGGGTGACA TCAAAAGCAA CATGAGTAAG
    CTACTTTATC AAACCCGTGG TGAGTCGGAC TGTCACTTCT CTGAAATCCA GTCTGAGGCC
    TCCACCGAGG AGGGTCAGGA CACGTACTCT GTGGGTAGTT TTGATGAAGA CTCCAAGTCT
    GAGTTTATTC TTGACCCACC GAAGCCCAAA CCACCAGCCT GGTATCATGG CATCTTGACC
    TGGGAGGACT TTGAACTGGT AAACCCTCAC AGAGCACTGT TTCTTAAAGA GCTGAAGGCA
    CTTTCTGTGA AGCGCAGGCA GATTCTAGGC AATAAGAGTC TGTCCGAGGA TGAGAAGAAC
    ACACGGCTTC AGGATCTCAT GCTGAAGAAT CCTATGGGCT CTGGCCCTCC ACTGTGTGTT
    GAAGATCTAG GATTAAATTT CCAGTTCTGT CCATCATCCA AAGTACATGG TTTCTCTTCT
    GTAGACTTGA AGCCTAATGG AGAGGATGAG ATGGTCACCA TGGACAATGC AGAGGAGTAT
    GTAGAGCTGA TGTTTGACTT CTGCATGCAC ACAGGAATCC AGAAGCAAAT GGAGGCCTTT
    AGAGAGGGCT TTAATAAAGT GTTCCCTATG GAGAAACTGA GCTCATTTAG CCATAAAGAG
    GTGCAGATGA TCCTGTGTGG AAACCAGTCT CCATCCTGGA CTGCTGAAGA CATTGTTAAC
    TACACAGAAC CTAAACTGGG CTATACACGG GACAGTCCTG GTTTCCTGCG TTTCGTGCGG
    GTTCTGTGTG GAATGTCTTC TGATGAGAGG AAAGCTTTCC TCCAGTTCAC TACAGGCTGC
    TCAACTCTGC CCCCTGGTGG ACTGGCCAAC CTCCATCCAC GGCTCACAAT AGTCCGAAAG
    GTTGATGCAA CAGATGCCAG CTATCCTTCA GTCAACACGT GTGTGCACTA CTTGAAGCTG
    CCGGAATATT CTTCAGAAGA GATTATGAGA GAGCGCCTTC TCGCTGCCAC TATGGAGAAG
    GGTTTCCACC TCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291386.1
    CDS463..8220
    Translation

    Target ORF information:

    RefSeq Version XM_009293111.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293111.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGGTTG CTGGTCTGTA GAGCATGTGG AACAGTACCT TGGCACAGAT
    GAATTACCAA AGAATGACTT GATAACCTAC ATGCAGAAGA ATGCAGACTC AACTTTCCTG
    CGCCACTGGA AATTAACCGG CTCTAATAAA AGTATTAGGA AAAACAGAAA TTGTTCGCAG
    CTCATAGCTG CATACAAGGA CTTCTGTGAG CGTGGCTGCA GATCTTCAGG CCTGAGTTCA
    GGGACGTTGT CCACGACGCA AAGCTGTGAT ATCTTGAGTG CTGCAAGAGA GCAGGCTCAG
    GCTAAAGCGG GCTCGGGTCA GAGCGCCTGC AGCGTAGAGG ATGTACTGCA GCTCTTGCGC
    ATCCTTTTCA CCATCGGAGG AGAACCTACA TCTGGACGCA CACTACAGGA AGATGTGGAA
    GAGCTACAGT TCAATGCATC ACCTGAGGAA TTCACCAGCA AAAAAATTAC AACCAAAATC
    CTGCAGCAGA TTGAAGAGCC ATTGGCTCTG GCTAGTGGGG CTCTCCCCGA CTGGTGTGAA
    CAGTTAACCA GCAAGTGTCC TTTCCTCATA CCCTTTGAAA CCCGGCAGCT TTATTTTACC
    TGCACTGCAT TTGGAGCCTC TAGGGCAATT GTTTGGCTCC AGAATCGAAG GGAAGCCACT
    ATGGAGCGTT CCCGGCCATC CACGACTGTT CGCCGTGATG ATCCCGGAGA GTTCCGTGTT
    GGTCGGCTTA AGCATGAACG TGTCAAGGTG CCTCGAGGAG AGAGTATGAT GGAGTGGGCT
    GAGAGTGTGA TGCAGATACA CGCTGACAGA AAGTCTGTAC TGGAGGTGGA GTTCCAAGGG
    GAGGAGGGCA CTGGTCTCGG ACCCACCCTG GAGTTCTATG CTCTTGTTGC GGCAGAGTTT
    CAGAGGACCT CCCTTGGCAT CTGGCTCTGT GATGATGACT TCCCAGATGA TGAGTCACGT
    CAGGTGGATC TGGGCGGTGG CTTGAAACCG CCAGGCTATT ACGTGCAGCG TTCCTGCGGC
    CTTTTCCCTG CTCCCTTCCC TCAGGACAGC GATGAACTGG AGCGTATTAC CAAACTTTTC
    TTGTTCCTTG GCATCTTTCT GGCCAAATGC ATCCAGGACA ACCGGCTCGT GGATCTGCCC
    ATATCCCAGC CTTTCTTTAA ACTGCTCTGT ATGGGTGACA TCAAAAGCAA CATGAGTAAG
    CTACTTTATC AAACCCGTGG TGAGTCGGAC TGTCACTTCT CTGAAATCCA GTCTGAGGCC
    TCCACCGAGG AGGGTCAGGA CACGTACTCT GTGGGTAGTT TTGATGAAGA CTCCAAGTCT
    GAGTTTATTC TTGACCCACC GAAGCCCAAA CCACCAGCCT GGTATCATGG CATCTTGACC
    TGGGAGGACT TTGAACTGGT AAACCCTCAC AGAGCACTGT TTCTTAAAGA GCTGAAGGCA
    CTTTCTGTGA AGCGCAGGCA GATTCTAGGC AATAAGAGTC TGTCCGAGGA TGAGAAGAAC
    ACACGGCTTC AGGATCTCAT GCTGAAGAAT CCTATGGGCT CTGGCCCTCC ACTGTGTGTT
    GAAGATCTAG GATTAAATTT CCAGTTCTGT CCATCATCCA AAGTACATGG TTTCTCTTCT
    GTAGACTTGA AGCCTAATGG AGAGGATGAG ATGGTCACCA TGGACAATGC AGAGGAGTAT
    GTAGAGCTGA TGTTTGACTT CTGCATGCAC ACAGGAATCC AGAAGCAAAT GGAGGCCTTT
    AGAGAGGGCT TTAATAAAGT GTTCCCTATG GAGAAACTGA GCTCATTTAG CCATAAAGAG
    GTGCAGATGA TCCTGTGTGG AAACCAGTCT CCATCCTGGA CTGCTGAAGA CATTGTTAAC
    TACACAGAAC CTAAACTGGG CTATACACGG GACAGTCCTG GTTTCCTGCG TTTCGTGCGG
    GTTCTGTGTG GAATGTCTTC TGATGAGAGG AAAGCTTTCC TCCAGTTCAC TACAGGCTGC
    TCAACTCTGC CCCCTGGTGG ACTGGCCAAC CTCCATCCAC GGCTCACAAT AGTCCGAAAG
    GTTGATGCAA CAGATGCCAG CTATCCTTCA GTCAACACGT GTGTGCACTA CTTGAAGCTG
    CCGGAATATT CTTCAGAAGA GATTATGAGA GAGCGCCTTC TCGCTGCCAC TATGGAGAAG
    GGTTTCCACC TCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47643
    Clone ID Related Accession (Same CDS sequence) XM_009293112.3 , XM_009293112.2
    Accession Version XM_009293112.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GGTTGCTGGT CTGTAGAGCA TGTGGAACAG
    TACCTTGGCA CAGATGAATT ACCAAAGAAT GACTTGATAA CCTACATGCA GAAGAATGCA
    GACTCAACTT TCCTGCGCCA CTGGAAATTA ACCGGCTCTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CGCAGCTCAT AGCTGCATAC AAGGACTTCT GTGAGCGTGG CTGCAGATCT
    TCAGGCCTGA GTTCAGGGAC GTTGTCCACG ACGCAAAGCT GTGATATCTT GAGTGCTGCA
    AGAGAGCAGG CTCAGGCTAA AGCGGGCTCG GGTCAGAGCG CCTGCAGCGT AGAGGATGTA
    CTGCAGCTCT TGCGCATCCT TTTCACCATC GGAGGAGAAC CTACATCTGG ACGCACACTA
    CAGGAAGATG TGGAAGAGCT ACAGTTCAAT GCATCACCTG AGGAATTCAC CAGCAAAAAA
    ATTACAACCA AAATCCTGCA GCAGATTGAA GAGCCATTGG CTCTGGCTAG TGGGGCTCTC
    CCCGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TCATACCCTT TGAAACCCGG
    CAGCTTTATT TTACCTGCAC TGCATTTGGA GCCTCTAGGG CAATTGTTTG GCTCCAGAAT
    CGAAGGGAAG CCACTATGGA GCGTTCCCGG CCATCCACGA CTGTTCGCCG TGATGATCCC
    GGAGAGTTCC GTGTTGGTCG GCTTAAGCAT GAACGTGTCA AGGTGCCTCG AGGAGAGAGT
    ATGATGGAGT GGGCTGAGAG TGTGATGCAG ATACACGCTG ACAGAAAGTC TGTACTGGAG
    GTGGAGTTCC AAGGGGAGGA GGGCACTGGT CTCGGACCCA CCCTGGAGTT CTATGCTCTT
    GTTGCGGCAG AGTTTCAGAG GACCTCCCTT GGCATCTGGC TCTGTGATGA TGACTTCCCA
    GATGATGAGT CACGTCAGGT GGATCTGGGC GGTGGCTTGA AACCGCCAGG CTATTACGTG
    CAGCGTTCCT GCGGCCTTTT CCCTGCTCCC TTCCCTCAGG ACAGCGATGA ACTGGAGCGT
    ATTACCAAAC TTTTCTTGTT CCTTGGCATC TTTCTGGCCA AATGCATCCA GGACAACCGG
    CTCGTGGATC TGCCCATATC CCAGCCTTTC TTTAAACTGC TCTGTATGGG TGACATCAAA
    AGCAACATGA GTAAGCTACT TTATCAAACC CGTGGTGAGT CGGACTGTCA CTTCTCTGAA
    ATCCAGTCTG AGGCCTCCAC CGAGGAGGGT CAGGACACGT ACTCTGTGGG TAGTTTTGAT
    GAAGACTCCA AGTCTGAGTT TATTCTTGAC CCACCGAAGC CCAAACCACC AGCCTGGTAT
    CATGGCATCT TGACCTGGGA GGACTTTGAA CTGGTAAACC CTCACAGAGC ACTGTTTCTT
    AAAGAGCTGA AGGCACTTTC TGTGAAGCGC AGGCAGATTC TAGGCAATAA GAGTCTGTCC
    GAGGATGAGA AGAACACACG GCTTCAGGAT CTCATGCTGA AGAATCCTAT GGGCTCTGGC
    CCTCCACTGT GTGTTGAAGA TCTAGGATTA AATTTCCAGT TCTGTCCATC ATCCAAAGTA
    CATGGTTTCT CTTCTGTAGA CTTGAAGCCT AATGGAGAGG ATGAGATGGT CACCATGGAC
    AATGCAGAGG AGTATGTAGA GCTGATGTTT GACTTCTGCA TGCACACAGG AATCCAGAAG
    CAAATGGAGG CCTTTAGAGA GGGCTTTAAT AAAGTGTTCC CTATGGAGAA ACTGAGCTCA
    TTTAGCCATA AAGAGGTGCA GATGATCCTG TGTGGAAACC AGTCTCCATC CTGGACTGCT
    GAAGACATTG TTAACTACAC AGAACCTAAA CTGGGCTATA CACGGGACAG TCCTGGTTTC
    CTGCGTTTCG TGCGGGTTCT GTGTGGAATG TCTTCTGATG AGAGGAAAGC TTTCCTCCAG
    TTCACTACAG GCTGCTCAAC TCTGCCCCCT GGTGGACTGG CCAACCTCCA TCCACGGCTC
    ACAATAGTCC GAAAGGTTGA TGCAACAGAT GCCAGCTATC CTTCAGTCAA CACGTGTGTG
    CACTACTTGA AGCTGCCGGA ATATTCTTCA GAAGAGATTA TGAGAGAGCG CCTTCTCGCT
    GCCACTATGG AGAAGGGTTT CCACCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291387.1
    CDS463..8175
    Translation

    Target ORF information:

    RefSeq Version XM_009293112.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293112.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GGTTGCTGGT CTGTAGAGCA TGTGGAACAG
    TACCTTGGCA CAGATGAATT ACCAAAGAAT GACTTGATAA CCTACATGCA GAAGAATGCA
    GACTCAACTT TCCTGCGCCA CTGGAAATTA ACCGGCTCTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CGCAGCTCAT AGCTGCATAC AAGGACTTCT GTGAGCGTGG CTGCAGATCT
    TCAGGCCTGA GTTCAGGGAC GTTGTCCACG ACGCAAAGCT GTGATATCTT GAGTGCTGCA
    AGAGAGCAGG CTCAGGCTAA AGCGGGCTCG GGTCAGAGCG CCTGCAGCGT AGAGGATGTA
    CTGCAGCTCT TGCGCATCCT TTTCACCATC GGAGGAGAAC CTACATCTGG ACGCACACTA
    CAGGAAGATG TGGAAGAGCT ACAGTTCAAT GCATCACCTG AGGAATTCAC CAGCAAAAAA
    ATTACAACCA AAATCCTGCA GCAGATTGAA GAGCCATTGG CTCTGGCTAG TGGGGCTCTC
    CCCGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TCATACCCTT TGAAACCCGG
    CAGCTTTATT TTACCTGCAC TGCATTTGGA GCCTCTAGGG CAATTGTTTG GCTCCAGAAT
    CGAAGGGAAG CCACTATGGA GCGTTCCCGG CCATCCACGA CTGTTCGCCG TGATGATCCC
    GGAGAGTTCC GTGTTGGTCG GCTTAAGCAT GAACGTGTCA AGGTGCCTCG AGGAGAGAGT
    ATGATGGAGT GGGCTGAGAG TGTGATGCAG ATACACGCTG ACAGAAAGTC TGTACTGGAG
    GTGGAGTTCC AAGGGGAGGA GGGCACTGGT CTCGGACCCA CCCTGGAGTT CTATGCTCTT
    GTTGCGGCAG AGTTTCAGAG GACCTCCCTT GGCATCTGGC TCTGTGATGA TGACTTCCCA
    GATGATGAGT CACGTCAGGT GGATCTGGGC GGTGGCTTGA AACCGCCAGG CTATTACGTG
    CAGCGTTCCT GCGGCCTTTT CCCTGCTCCC TTCCCTCAGG ACAGCGATGA ACTGGAGCGT
    ATTACCAAAC TTTTCTTGTT CCTTGGCATC TTTCTGGCCA AATGCATCCA GGACAACCGG
    CTCGTGGATC TGCCCATATC CCAGCCTTTC TTTAAACTGC TCTGTATGGG TGACATCAAA
    AGCAACATGA GTAAGCTACT TTATCAAACC CGTGGTGAGT CGGACTGTCA CTTCTCTGAA
    ATCCAGTCTG AGGCCTCCAC CGAGGAGGGT CAGGACACGT ACTCTGTGGG TAGTTTTGAT
    GAAGACTCCA AGTCTGAGTT TATTCTTGAC CCACCGAAGC CCAAACCACC AGCCTGGTAT
    CATGGCATCT TGACCTGGGA GGACTTTGAA CTGGTAAACC CTCACAGAGC ACTGTTTCTT
    AAAGAGCTGA AGGCACTTTC TGTGAAGCGC AGGCAGATTC TAGGCAATAA GAGTCTGTCC
    GAGGATGAGA AGAACACACG GCTTCAGGAT CTCATGCTGA AGAATCCTAT GGGCTCTGGC
    CCTCCACTGT GTGTTGAAGA TCTAGGATTA AATTTCCAGT TCTGTCCATC ATCCAAAGTA
    CATGGTTTCT CTTCTGTAGA CTTGAAGCCT AATGGAGAGG ATGAGATGGT CACCATGGAC
    AATGCAGAGG AGTATGTAGA GCTGATGTTT GACTTCTGCA TGCACACAGG AATCCAGAAG
    CAAATGGAGG CCTTTAGAGA GGGCTTTAAT AAAGTGTTCC CTATGGAGAA ACTGAGCTCA
    TTTAGCCATA AAGAGGTGCA GATGATCCTG TGTGGAAACC AGTCTCCATC CTGGACTGCT
    GAAGACATTG TTAACTACAC AGAACCTAAA CTGGGCTATA CACGGGACAG TCCTGGTTTC
    CTGCGTTTCG TGCGGGTTCT GTGTGGAATG TCTTCTGATG AGAGGAAAGC TTTCCTCCAG
    TTCACTACAG GCTGCTCAAC TCTGCCCCCT GGTGGACTGG CCAACCTCCA TCCACGGCTC
    ACAATAGTCC GAAAGGTTGA TGCAACAGAT GCCAGCTATC CTTCAGTCAA CACGTGTGTG
    CACTACTTGA AGCTGCCGGA ATATTCTTCA GAAGAGATTA TGAGAGAGCG CCTTCTCGCT
    GCCACTATGG AGAAGGGTTT CCACCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47643
    Clone ID Related Accession (Same CDS sequence) XM_009293112.3 , XM_009293112.2
    Accession Version XM_009293112.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GGTTGCTGGT CTGTAGAGCA TGTGGAACAG
    TACCTTGGCA CAGATGAATT ACCAAAGAAT GACTTGATAA CCTACATGCA GAAGAATGCA
    GACTCAACTT TCCTGCGCCA CTGGAAATTA ACCGGCTCTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CGCAGCTCAT AGCTGCATAC AAGGACTTCT GTGAGCGTGG CTGCAGATCT
    TCAGGCCTGA GTTCAGGGAC GTTGTCCACG ACGCAAAGCT GTGATATCTT GAGTGCTGCA
    AGAGAGCAGG CTCAGGCTAA AGCGGGCTCG GGTCAGAGCG CCTGCAGCGT AGAGGATGTA
    CTGCAGCTCT TGCGCATCCT TTTCACCATC GGAGGAGAAC CTACATCTGG ACGCACACTA
    CAGGAAGATG TGGAAGAGCT ACAGTTCAAT GCATCACCTG AGGAATTCAC CAGCAAAAAA
    ATTACAACCA AAATCCTGCA GCAGATTGAA GAGCCATTGG CTCTGGCTAG TGGGGCTCTC
    CCCGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TCATACCCTT TGAAACCCGG
    CAGCTTTATT TTACCTGCAC TGCATTTGGA GCCTCTAGGG CAATTGTTTG GCTCCAGAAT
    CGAAGGGAAG CCACTATGGA GCGTTCCCGG CCATCCACGA CTGTTCGCCG TGATGATCCC
    GGAGAGTTCC GTGTTGGTCG GCTTAAGCAT GAACGTGTCA AGGTGCCTCG AGGAGAGAGT
    ATGATGGAGT GGGCTGAGAG TGTGATGCAG ATACACGCTG ACAGAAAGTC TGTACTGGAG
    GTGGAGTTCC AAGGGGAGGA GGGCACTGGT CTCGGACCCA CCCTGGAGTT CTATGCTCTT
    GTTGCGGCAG AGTTTCAGAG GACCTCCCTT GGCATCTGGC TCTGTGATGA TGACTTCCCA
    GATGATGAGT CACGTCAGGT GGATCTGGGC GGTGGCTTGA AACCGCCAGG CTATTACGTG
    CAGCGTTCCT GCGGCCTTTT CCCTGCTCCC TTCCCTCAGG ACAGCGATGA ACTGGAGCGT
    ATTACCAAAC TTTTCTTGTT CCTTGGCATC TTTCTGGCCA AATGCATCCA GGACAACCGG
    CTCGTGGATC TGCCCATATC CCAGCCTTTC TTTAAACTGC TCTGTATGGG TGACATCAAA
    AGCAACATGA GTAAGCTACT TTATCAAACC CGTGGTGAGT CGGACTGTCA CTTCTCTGAA
    ATCCAGTCTG AGGCCTCCAC CGAGGAGGGT CAGGACACGT ACTCTGTGGG TAGTTTTGAT
    GAAGACTCCA AGTCTGAGTT TATTCTTGAC CCACCGAAGC CCAAACCACC AGCCTGGTAT
    CATGGCATCT TGACCTGGGA GGACTTTGAA CTGGTAAACC CTCACAGAGC ACTGTTTCTT
    AAAGAGCTGA AGGCACTTTC TGTGAAGCGC AGGCAGATTC TAGGCAATAA GAGTCTGTCC
    GAGGATGAGA AGAACACACG GCTTCAGGAT CTCATGCTGA AGAATCCTAT GGGCTCTGGC
    CCTCCACTGT GTGTTGAAGA TCTAGGATTA AATTTCCAGT TCTGTCCATC ATCCAAAGTA
    CATGGTTTCT CTTCTGTAGA CTTGAAGCCT AATGGAGAGG ATGAGATGGT CACCATGGAC
    AATGCAGAGG AGTATGTAGA GCTGATGTTT GACTTCTGCA TGCACACAGG AATCCAGAAG
    CAAATGGAGG CCTTTAGAGA GGGCTTTAAT AAAGTGTTCC CTATGGAGAA ACTGAGCTCA
    TTTAGCCATA AAGAGGTGCA GATGATCCTG TGTGGAAACC AGTCTCCATC CTGGACTGCT
    GAAGACATTG TTAACTACAC AGAACCTAAA CTGGGCTATA CACGGGACAG TCCTGGTTTC
    CTGCGTTTCG TGCGGGTTCT GTGTGGAATG TCTTCTGATG AGAGGAAAGC TTTCCTCCAG
    TTCACTACAG GCTGCTCAAC TCTGCCCCCT GGTGGACTGG CCAACCTCCA TCCACGGCTC
    ACAATAGTCC GAAAGGTTGA TGCAACAGAT GCCAGCTATC CTTCAGTCAA CACGTGTGTG
    CACTACTTGA AGCTGCCGGA ATATTCTTCA GAAGAGATTA TGAGAGAGCG CCTTCTCGCT
    GCCACTATGG AGAAGGGTTT CCACCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291387.1
    CDS463..8175
    Translation

    Target ORF information:

    RefSeq Version XM_009293112.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GGTTGCTGGT CTGTAGAGCA TGTGGAACAG
    TACCTTGGCA CAGATGAATT ACCAAAGAAT GACTTGATAA CCTACATGCA GAAGAATGCA
    GACTCAACTT TCCTGCGCCA CTGGAAATTA ACCGGCTCTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CGCAGCTCAT AGCTGCATAC AAGGACTTCT GTGAGCGTGG CTGCAGATCT
    TCAGGCCTGA GTTCAGGGAC GTTGTCCACG ACGCAAAGCT GTGATATCTT GAGTGCTGCA
    AGAGAGCAGG CTCAGGCTAA AGCGGGCTCG GGTCAGAGCG CCTGCAGCGT AGAGGATGTA
    CTGCAGCTCT TGCGCATCCT TTTCACCATC GGAGGAGAAC CTACATCTGG ACGCACACTA
    CAGGAAGATG TGGAAGAGCT ACAGTTCAAT GCATCACCTG AGGAATTCAC CAGCAAAAAA
    ATTACAACCA AAATCCTGCA GCAGATTGAA GAGCCATTGG CTCTGGCTAG TGGGGCTCTC
    CCCGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TCATACCCTT TGAAACCCGG
    CAGCTTTATT TTACCTGCAC TGCATTTGGA GCCTCTAGGG CAATTGTTTG GCTCCAGAAT
    CGAAGGGAAG CCACTATGGA GCGTTCCCGG CCATCCACGA CTGTTCGCCG TGATGATCCC
    GGAGAGTTCC GTGTTGGTCG GCTTAAGCAT GAACGTGTCA AGGTGCCTCG AGGAGAGAGT
    ATGATGGAGT GGGCTGAGAG TGTGATGCAG ATACACGCTG ACAGAAAGTC TGTACTGGAG
    GTGGAGTTCC AAGGGGAGGA GGGCACTGGT CTCGGACCCA CCCTGGAGTT CTATGCTCTT
    GTTGCGGCAG AGTTTCAGAG GACCTCCCTT GGCATCTGGC TCTGTGATGA TGACTTCCCA
    GATGATGAGT CACGTCAGGT GGATCTGGGC GGTGGCTTGA AACCGCCAGG CTATTACGTG
    CAGCGTTCCT GCGGCCTTTT CCCTGCTCCC TTCCCTCAGG ACAGCGATGA ACTGGAGCGT
    ATTACCAAAC TTTTCTTGTT CCTTGGCATC TTTCTGGCCA AATGCATCCA GGACAACCGG
    CTCGTGGATC TGCCCATATC CCAGCCTTTC TTTAAACTGC TCTGTATGGG TGACATCAAA
    AGCAACATGA GTAAGCTACT TTATCAAACC CGTGGTGAGT CGGACTGTCA CTTCTCTGAA
    ATCCAGTCTG AGGCCTCCAC CGAGGAGGGT CAGGACACGT ACTCTGTGGG TAGTTTTGAT
    GAAGACTCCA AGTCTGAGTT TATTCTTGAC CCACCGAAGC CCAAACCACC AGCCTGGTAT
    CATGGCATCT TGACCTGGGA GGACTTTGAA CTGGTAAACC CTCACAGAGC ACTGTTTCTT
    AAAGAGCTGA AGGCACTTTC TGTGAAGCGC AGGCAGATTC TAGGCAATAA GAGTCTGTCC
    GAGGATGAGA AGAACACACG GCTTCAGGAT CTCATGCTGA AGAATCCTAT GGGCTCTGGC
    CCTCCACTGT GTGTTGAAGA TCTAGGATTA AATTTCCAGT TCTGTCCATC ATCCAAAGTA
    CATGGTTTCT CTTCTGTAGA CTTGAAGCCT AATGGAGAGG ATGAGATGGT CACCATGGAC
    AATGCAGAGG AGTATGTAGA GCTGATGTTT GACTTCTGCA TGCACACAGG AATCCAGAAG
    CAAATGGAGG CCTTTAGAGA GGGCTTTAAT AAAGTGTTCC CTATGGAGAA ACTGAGCTCA
    TTTAGCCATA AAGAGGTGCA GATGATCCTG TGTGGAAACC AGTCTCCATC CTGGACTGCT
    GAAGACATTG TTAACTACAC AGAACCTAAA CTGGGCTATA CACGGGACAG TCCTGGTTTC
    CTGCGTTTCG TGCGGGTTCT GTGTGGAATG TCTTCTGATG AGAGGAAAGC TTTCCTCCAG
    TTCACTACAG GCTGCTCAAC TCTGCCCCCT GGTGGACTGG CCAACCTCCA TCCACGGCTC
    ACAATAGTCC GAAAGGTTGA TGCAACAGAT GCCAGCTATC CTTCAGTCAA CACGTGTGTG
    CACTACTTGA AGCTGCCGGA ATATTCTTCA GAAGAGATTA TGAGAGAGCG CCTTCTCGCT
    GCCACTATGG AGAAGGGTTT CCACCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47644
    Clone ID Related Accession (Same CDS sequence) XM_009293113.2 , XM_009293113.3
    Accession Version XM_009293113.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGTACGG
    TCCAGGGTCA ACCTTGGTTA CCACGTTCAT ATTCATAGGG AGGAAGAGGA GTATGAAACC
    AAAGGGGGCC GTCGCAGGAC CTGGGATGAC GACTTTGTGC TGAAACGACA GTTTTCTGCT
    TTAGTACCTG CATTTGACCC TAGACCAGGC AGGACCAACG TCCAGCAAAC TACTGACCTG
    GAAATCCCCC CACCAGGAAC ACCTCGTTCA GAGGTACAGG AGGAGGTGGA GTGCGCTCCT
    TCACCTCGTC TGGCTCTCAT CTTAAAAGTA GCAGGTCTAG GAACTACTCG AGAAGTAGAA
    CTACCTCTCA CCAATTACAA GTCCACCATT TTCTATTATG TCCAAAAACT TCTCCAGCTG
    TCATGCAACG GTGCTATTAA ACCTGACAAG CTCAGACGCA TCTGGGAGCC TACGTATACG
    ATAATGTATA GAGAATTGAA GGATTCCGAC AAAGAAAGAG AGAGCGGAAA GATGGGTTGC
    TGGTCTGTAG AGCATGTGGA ACAGTACCTT GGCACAGATG AATTACCAAA GAATGACTTG
    ATAACCTACA TGCAGAAGAA TGCAGACTCA ACTTTCCTGC GCCACTGGAA ATTAACCGGC
    TCTAATAAAA GTATTAGGAA AAACAGAAAT TGTTCGCAGC TCATAGCTGC ATACAAGGAC
    TTCTGTGAGC GTGGCTGCAG ATCTTCAGGC CTGAGTTCAG GGACGTTGTC CACGACGCAA
    AGCTGTGATA TCTTGAGTGC TGCAAGAGAG CAGGCTCAGG CTAAAGCGGG CTCGGGTCAG
    AGCGCCTGCA GCGTAGAGGA TGTACTGCAG CTCTTGCGCA TCCTTTTCAC CATCGGAGGA
    GAACCTACAT CTGGACGCAC ACTACAGGAA GATGTGGAAG AGCTACAGTT CAATGCATCA
    CCTGAGGAAT TCACCAGCAA AAAAATTACA ACCAAAATCC TGCAGCAGAT TGAAGAGCCA
    TTGGCTCTGG CTAGTGGGGC TCTCCCCGAC TGGTGTGAAC AGTTAACCAG CAAGTGTCCT
    TTCCTCATAC CCTTTGAAAC CCGGCAGCTT TATTTTACCT GCACTGCATT TGGAGCCTCT
    AGGGCAATTG TTTGGCTCCA GAATCGAAGG GAAGCCACTA TGGAGCGTTC CCGGCCATCC
    ACGACTGTTC GCCGTGATGA TCCCGGAGAG TTCCGTGTTG GTCGGCTTAA GCATGAACGT
    GTCAAGGTGC CTCGAGGAGA GAGTATGATG GAGTGGGCTG AGAGTGTGAT GCAGATACAC
    GCTGACAGAA AGTCTGTACT GGAGGTGGAG TTCCAAGGGG AGGAGGGCAC TGGTCTCGGA
    CCCACCCTGG AGTTCTATGC TCTTGTTGCG GCAGAGTTTC AGAGGACCTC CCTTGGCATC
    TGGCTCTGTG ATGATGACTT CCCAGATGAT GAGTCACGTC AGGTGGATCT GGGCGGTGGC
    TTGAAACCGC CAGGCTATTA CGTGCAGCGT TCCTGCGGCC TTTTCCCTGC TCCCTTCCCT
    CAGGACAGCG ATGAACTGGA GCGTATTACC AAACTTTTCT TGTTCCTTGG CATCTTTCTG
    GCCAAATGCA TCCAGGACAA CCGGCTCGTG GATCTGCCCA TATCCCAGCC TTTCTTTAAA
    CTGCTCTGTA TGGGTGACAT CAAAAGCAAC ATGAGTAAGC TACTTTATCA AACCCGTGGT
    GAGTCGGACT GTCACTTCTC TGAAATCCAG TCTGAGGCCT CCACCGAGGA GGGTCAGGAC
    ACGTACTCTG TGGGTAGTTT TGATGAAGAC TCCAAGTCTG AGTTTATTCT TGACCCACCG
    AAGCCCAAAC CACCAGCCTG GTATCATGGC ATCTTGACCT GGGAGGACTT TGAACTGGTA
    AACCCTCACA GAGCACTGTT TCTTAAAGAG CTGAAGGCAC TTTCTGTGAA GCGCAGGCAG
    ATTCTAGGCA ATAAGAGTCT GTCCGAGGAT GAGAAGAACA CACGGCTTCA GGATCTCATG
    CTGAAGAATC CTATGGGCTC TGGCCCTCCA CTGTGTGTTG AAGATCTAGG ATTAAATTTC
    CAGTTCTGTC CATCATCCAA AGTACATGGT TTCTCTTCTG TAGACTTGAA GCCTAATGGA
    GAGGATGAGA TGGTCACCAT GGACAATGCA GAGGAGTATG TAGAGCTGAT GTTTGACTTC
    TGCATGCACA CAGGAATCCA GAAGCAAATG GAGGCCTTTA GAGAGGGCTT TAATAAAGTG
    TTCCCTATGG AGAAACTGAG CTCATTTAGC CATAAAGAGG TGCAGATGAT CCTGTGTGGA
    AACCAGTCTC CATCCTGGAC TGCTGAAGAC ATTGTTAACT ACACAGAACC TAAACTGGGC
    TATACACGGG ACAGTCCTGG TTTCCTGCGT TTCGTGCGGG TTCTGTGTGG AATGTCTTCT
    GATGAGAGGA AAGCTTTCCT CCAGTTCACT ACAGGCTGCT CAACTCTGCC CCCTGGTGGA
    CTGGCCAACC TCCATCCACG GCTCACAATA GTCCGAAAGG TTGATGCAAC AGATGCCAGC
    TATCCTTCAG TCAACACGTG TGTGCACTAC TTGAAGCTGC CGGAATATTC TTCAGAAGAG
    ATTATGAGAG AGCGCCTTCT CGCTGCCACT ATGGAGAAGG GTTTCCACCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291388.1
    CDS463..8079
    Translation

    Target ORF information:

    RefSeq Version XM_009293113.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGTACGG
    TCCAGGGTCA ACCTTGGTTA CCACGTTCAT ATTCATAGGG AGGAAGAGGA GTATGAAACC
    AAAGGGGGCC GTCGCAGGAC CTGGGATGAC GACTTTGTGC TGAAACGACA GTTTTCTGCT
    TTAGTACCTG CATTTGACCC TAGACCAGGC AGGACCAACG TCCAGCAAAC TACTGACCTG
    GAAATCCCCC CACCAGGAAC ACCTCGTTCA GAGGTACAGG AGGAGGTGGA GTGCGCTCCT
    TCACCTCGTC TGGCTCTCAT CTTAAAAGTA GCAGGTCTAG GAACTACTCG AGAAGTAGAA
    CTACCTCTCA CCAATTACAA GTCCACCATT TTCTATTATG TCCAAAAACT TCTCCAGCTG
    TCATGCAACG GTGCTATTAA ACCTGACAAG CTCAGACGCA TCTGGGAGCC TACGTATACG
    ATAATGTATA GAGAATTGAA GGATTCCGAC AAAGAAAGAG AGAGCGGAAA GATGGGTTGC
    TGGTCTGTAG AGCATGTGGA ACAGTACCTT GGCACAGATG AATTACCAAA GAATGACTTG
    ATAACCTACA TGCAGAAGAA TGCAGACTCA ACTTTCCTGC GCCACTGGAA ATTAACCGGC
    TCTAATAAAA GTATTAGGAA AAACAGAAAT TGTTCGCAGC TCATAGCTGC ATACAAGGAC
    TTCTGTGAGC GTGGCTGCAG ATCTTCAGGC CTGAGTTCAG GGACGTTGTC CACGACGCAA
    AGCTGTGATA TCTTGAGTGC TGCAAGAGAG CAGGCTCAGG CTAAAGCGGG CTCGGGTCAG
    AGCGCCTGCA GCGTAGAGGA TGTACTGCAG CTCTTGCGCA TCCTTTTCAC CATCGGAGGA
    GAACCTACAT CTGGACGCAC ACTACAGGAA GATGTGGAAG AGCTACAGTT CAATGCATCA
    CCTGAGGAAT TCACCAGCAA AAAAATTACA ACCAAAATCC TGCAGCAGAT TGAAGAGCCA
    TTGGCTCTGG CTAGTGGGGC TCTCCCCGAC TGGTGTGAAC AGTTAACCAG CAAGTGTCCT
    TTCCTCATAC CCTTTGAAAC CCGGCAGCTT TATTTTACCT GCACTGCATT TGGAGCCTCT
    AGGGCAATTG TTTGGCTCCA GAATCGAAGG GAAGCCACTA TGGAGCGTTC CCGGCCATCC
    ACGACTGTTC GCCGTGATGA TCCCGGAGAG TTCCGTGTTG GTCGGCTTAA GCATGAACGT
    GTCAAGGTGC CTCGAGGAGA GAGTATGATG GAGTGGGCTG AGAGTGTGAT GCAGATACAC
    GCTGACAGAA AGTCTGTACT GGAGGTGGAG TTCCAAGGGG AGGAGGGCAC TGGTCTCGGA
    CCCACCCTGG AGTTCTATGC TCTTGTTGCG GCAGAGTTTC AGAGGACCTC CCTTGGCATC
    TGGCTCTGTG ATGATGACTT CCCAGATGAT GAGTCACGTC AGGTGGATCT GGGCGGTGGC
    TTGAAACCGC CAGGCTATTA CGTGCAGCGT TCCTGCGGCC TTTTCCCTGC TCCCTTCCCT
    CAGGACAGCG ATGAACTGGA GCGTATTACC AAACTTTTCT TGTTCCTTGG CATCTTTCTG
    GCCAAATGCA TCCAGGACAA CCGGCTCGTG GATCTGCCCA TATCCCAGCC TTTCTTTAAA
    CTGCTCTGTA TGGGTGACAT CAAAAGCAAC ATGAGTAAGC TACTTTATCA AACCCGTGGT
    GAGTCGGACT GTCACTTCTC TGAAATCCAG TCTGAGGCCT CCACCGAGGA GGGTCAGGAC
    ACGTACTCTG TGGGTAGTTT TGATGAAGAC TCCAAGTCTG AGTTTATTCT TGACCCACCG
    AAGCCCAAAC CACCAGCCTG GTATCATGGC ATCTTGACCT GGGAGGACTT TGAACTGGTA
    AACCCTCACA GAGCACTGTT TCTTAAAGAG CTGAAGGCAC TTTCTGTGAA GCGCAGGCAG
    ATTCTAGGCA ATAAGAGTCT GTCCGAGGAT GAGAAGAACA CACGGCTTCA GGATCTCATG
    CTGAAGAATC CTATGGGCTC TGGCCCTCCA CTGTGTGTTG AAGATCTAGG ATTAAATTTC
    CAGTTCTGTC CATCATCCAA AGTACATGGT TTCTCTTCTG TAGACTTGAA GCCTAATGGA
    GAGGATGAGA TGGTCACCAT GGACAATGCA GAGGAGTATG TAGAGCTGAT GTTTGACTTC
    TGCATGCACA CAGGAATCCA GAAGCAAATG GAGGCCTTTA GAGAGGGCTT TAATAAAGTG
    TTCCCTATGG AGAAACTGAG CTCATTTAGC CATAAAGAGG TGCAGATGAT CCTGTGTGGA
    AACCAGTCTC CATCCTGGAC TGCTGAAGAC ATTGTTAACT ACACAGAACC TAAACTGGGC
    TATACACGGG ACAGTCCTGG TTTCCTGCGT TTCGTGCGGG TTCTGTGTGG AATGTCTTCT
    GATGAGAGGA AAGCTTTCCT CCAGTTCACT ACAGGCTGCT CAACTCTGCC CCCTGGTGGA
    CTGGCCAACC TCCATCCACG GCTCACAATA GTCCGAAAGG TTGATGCAAC AGATGCCAGC
    TATCCTTCAG TCAACACGTG TGTGCACTAC TTGAAGCTGC CGGAATATTC TTCAGAAGAG
    ATTATGAGAG AGCGCCTTCT CGCTGCCACT ATGGAGAAGG GTTTCCACCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47644
    Clone ID Related Accession (Same CDS sequence) XM_009293113.2 , XM_009293113.3
    Accession Version XM_009293113.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGTACGG
    TCCAGGGTCA ACCTTGGTTA CCACGTTCAT ATTCATAGGG AGGAAGAGGA GTATGAAACC
    AAAGGGGGCC GTCGCAGGAC CTGGGATGAC GACTTTGTGC TGAAACGACA GTTTTCTGCT
    TTAGTACCTG CATTTGACCC TAGACCAGGC AGGACCAACG TCCAGCAAAC TACTGACCTG
    GAAATCCCCC CACCAGGAAC ACCTCGTTCA GAGGTACAGG AGGAGGTGGA GTGCGCTCCT
    TCACCTCGTC TGGCTCTCAT CTTAAAAGTA GCAGGTCTAG GAACTACTCG AGAAGTAGAA
    CTACCTCTCA CCAATTACAA GTCCACCATT TTCTATTATG TCCAAAAACT TCTCCAGCTG
    TCATGCAACG GTGCTATTAA ACCTGACAAG CTCAGACGCA TCTGGGAGCC TACGTATACG
    ATAATGTATA GAGAATTGAA GGATTCCGAC AAAGAAAGAG AGAGCGGAAA GATGGGTTGC
    TGGTCTGTAG AGCATGTGGA ACAGTACCTT GGCACAGATG AATTACCAAA GAATGACTTG
    ATAACCTACA TGCAGAAGAA TGCAGACTCA ACTTTCCTGC GCCACTGGAA ATTAACCGGC
    TCTAATAAAA GTATTAGGAA AAACAGAAAT TGTTCGCAGC TCATAGCTGC ATACAAGGAC
    TTCTGTGAGC GTGGCTGCAG ATCTTCAGGC CTGAGTTCAG GGACGTTGTC CACGACGCAA
    AGCTGTGATA TCTTGAGTGC TGCAAGAGAG CAGGCTCAGG CTAAAGCGGG CTCGGGTCAG
    AGCGCCTGCA GCGTAGAGGA TGTACTGCAG CTCTTGCGCA TCCTTTTCAC CATCGGAGGA
    GAACCTACAT CTGGACGCAC ACTACAGGAA GATGTGGAAG AGCTACAGTT CAATGCATCA
    CCTGAGGAAT TCACCAGCAA AAAAATTACA ACCAAAATCC TGCAGCAGAT TGAAGAGCCA
    TTGGCTCTGG CTAGTGGGGC TCTCCCCGAC TGGTGTGAAC AGTTAACCAG CAAGTGTCCT
    TTCCTCATAC CCTTTGAAAC CCGGCAGCTT TATTTTACCT GCACTGCATT TGGAGCCTCT
    AGGGCAATTG TTTGGCTCCA GAATCGAAGG GAAGCCACTA TGGAGCGTTC CCGGCCATCC
    ACGACTGTTC GCCGTGATGA TCCCGGAGAG TTCCGTGTTG GTCGGCTTAA GCATGAACGT
    GTCAAGGTGC CTCGAGGAGA GAGTATGATG GAGTGGGCTG AGAGTGTGAT GCAGATACAC
    GCTGACAGAA AGTCTGTACT GGAGGTGGAG TTCCAAGGGG AGGAGGGCAC TGGTCTCGGA
    CCCACCCTGG AGTTCTATGC TCTTGTTGCG GCAGAGTTTC AGAGGACCTC CCTTGGCATC
    TGGCTCTGTG ATGATGACTT CCCAGATGAT GAGTCACGTC AGGTGGATCT GGGCGGTGGC
    TTGAAACCGC CAGGCTATTA CGTGCAGCGT TCCTGCGGCC TTTTCCCTGC TCCCTTCCCT
    CAGGACAGCG ATGAACTGGA GCGTATTACC AAACTTTTCT TGTTCCTTGG CATCTTTCTG
    GCCAAATGCA TCCAGGACAA CCGGCTCGTG GATCTGCCCA TATCCCAGCC TTTCTTTAAA
    CTGCTCTGTA TGGGTGACAT CAAAAGCAAC ATGAGTAAGC TACTTTATCA AACCCGTGGT
    GAGTCGGACT GTCACTTCTC TGAAATCCAG TCTGAGGCCT CCACCGAGGA GGGTCAGGAC
    ACGTACTCTG TGGGTAGTTT TGATGAAGAC TCCAAGTCTG AGTTTATTCT TGACCCACCG
    AAGCCCAAAC CACCAGCCTG GTATCATGGC ATCTTGACCT GGGAGGACTT TGAACTGGTA
    AACCCTCACA GAGCACTGTT TCTTAAAGAG CTGAAGGCAC TTTCTGTGAA GCGCAGGCAG
    ATTCTAGGCA ATAAGAGTCT GTCCGAGGAT GAGAAGAACA CACGGCTTCA GGATCTCATG
    CTGAAGAATC CTATGGGCTC TGGCCCTCCA CTGTGTGTTG AAGATCTAGG ATTAAATTTC
    CAGTTCTGTC CATCATCCAA AGTACATGGT TTCTCTTCTG TAGACTTGAA GCCTAATGGA
    GAGGATGAGA TGGTCACCAT GGACAATGCA GAGGAGTATG TAGAGCTGAT GTTTGACTTC
    TGCATGCACA CAGGAATCCA GAAGCAAATG GAGGCCTTTA GAGAGGGCTT TAATAAAGTG
    TTCCCTATGG AGAAACTGAG CTCATTTAGC CATAAAGAGG TGCAGATGAT CCTGTGTGGA
    AACCAGTCTC CATCCTGGAC TGCTGAAGAC ATTGTTAACT ACACAGAACC TAAACTGGGC
    TATACACGGG ACAGTCCTGG TTTCCTGCGT TTCGTGCGGG TTCTGTGTGG AATGTCTTCT
    GATGAGAGGA AAGCTTTCCT CCAGTTCACT ACAGGCTGCT CAACTCTGCC CCCTGGTGGA
    CTGGCCAACC TCCATCCACG GCTCACAATA GTCCGAAAGG TTGATGCAAC AGATGCCAGC
    TATCCTTCAG TCAACACGTG TGTGCACTAC TTGAAGCTGC CGGAATATTC TTCAGAAGAG
    ATTATGAGAG AGCGCCTTCT CGCTGCCACT ATGGAGAAGG GTTTCCACCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291388.1
    CDS463..8079
    Translation

    Target ORF information:

    RefSeq Version XM_009293113.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGTACGG
    TCCAGGGTCA ACCTTGGTTA CCACGTTCAT ATTCATAGGG AGGAAGAGGA GTATGAAACC
    AAAGGGGGCC GTCGCAGGAC CTGGGATGAC GACTTTGTGC TGAAACGACA GTTTTCTGCT
    TTAGTACCTG CATTTGACCC TAGACCAGGC AGGACCAACG TCCAGCAAAC TACTGACCTG
    GAAATCCCCC CACCAGGAAC ACCTCGTTCA GAGGTACAGG AGGAGGTGGA GTGCGCTCCT
    TCACCTCGTC TGGCTCTCAT CTTAAAAGTA GCAGGTCTAG GAACTACTCG AGAAGTAGAA
    CTACCTCTCA CCAATTACAA GTCCACCATT TTCTATTATG TCCAAAAACT TCTCCAGCTG
    TCATGCAACG GTGCTATTAA ACCTGACAAG CTCAGACGCA TCTGGGAGCC TACGTATACG
    ATAATGTATA GAGAATTGAA GGATTCCGAC AAAGAAAGAG AGAGCGGAAA GATGGGTTGC
    TGGTCTGTAG AGCATGTGGA ACAGTACCTT GGCACAGATG AATTACCAAA GAATGACTTG
    ATAACCTACA TGCAGAAGAA TGCAGACTCA ACTTTCCTGC GCCACTGGAA ATTAACCGGC
    TCTAATAAAA GTATTAGGAA AAACAGAAAT TGTTCGCAGC TCATAGCTGC ATACAAGGAC
    TTCTGTGAGC GTGGCTGCAG ATCTTCAGGC CTGAGTTCAG GGACGTTGTC CACGACGCAA
    AGCTGTGATA TCTTGAGTGC TGCAAGAGAG CAGGCTCAGG CTAAAGCGGG CTCGGGTCAG
    AGCGCCTGCA GCGTAGAGGA TGTACTGCAG CTCTTGCGCA TCCTTTTCAC CATCGGAGGA
    GAACCTACAT CTGGACGCAC ACTACAGGAA GATGTGGAAG AGCTACAGTT CAATGCATCA
    CCTGAGGAAT TCACCAGCAA AAAAATTACA ACCAAAATCC TGCAGCAGAT TGAAGAGCCA
    TTGGCTCTGG CTAGTGGGGC TCTCCCCGAC TGGTGTGAAC AGTTAACCAG CAAGTGTCCT
    TTCCTCATAC CCTTTGAAAC CCGGCAGCTT TATTTTACCT GCACTGCATT TGGAGCCTCT
    AGGGCAATTG TTTGGCTCCA GAATCGAAGG GAAGCCACTA TGGAGCGTTC CCGGCCATCC
    ACGACTGTTC GCCGTGATGA TCCCGGAGAG TTCCGTGTTG GTCGGCTTAA GCATGAACGT
    GTCAAGGTGC CTCGAGGAGA GAGTATGATG GAGTGGGCTG AGAGTGTGAT GCAGATACAC
    GCTGACAGAA AGTCTGTACT GGAGGTGGAG TTCCAAGGGG AGGAGGGCAC TGGTCTCGGA
    CCCACCCTGG AGTTCTATGC TCTTGTTGCG GCAGAGTTTC AGAGGACCTC CCTTGGCATC
    TGGCTCTGTG ATGATGACTT CCCAGATGAT GAGTCACGTC AGGTGGATCT GGGCGGTGGC
    TTGAAACCGC CAGGCTATTA CGTGCAGCGT TCCTGCGGCC TTTTCCCTGC TCCCTTCCCT
    CAGGACAGCG ATGAACTGGA GCGTATTACC AAACTTTTCT TGTTCCTTGG CATCTTTCTG
    GCCAAATGCA TCCAGGACAA CCGGCTCGTG GATCTGCCCA TATCCCAGCC TTTCTTTAAA
    CTGCTCTGTA TGGGTGACAT CAAAAGCAAC ATGAGTAAGC TACTTTATCA AACCCGTGGT
    GAGTCGGACT GTCACTTCTC TGAAATCCAG TCTGAGGCCT CCACCGAGGA GGGTCAGGAC
    ACGTACTCTG TGGGTAGTTT TGATGAAGAC TCCAAGTCTG AGTTTATTCT TGACCCACCG
    AAGCCCAAAC CACCAGCCTG GTATCATGGC ATCTTGACCT GGGAGGACTT TGAACTGGTA
    AACCCTCACA GAGCACTGTT TCTTAAAGAG CTGAAGGCAC TTTCTGTGAA GCGCAGGCAG
    ATTCTAGGCA ATAAGAGTCT GTCCGAGGAT GAGAAGAACA CACGGCTTCA GGATCTCATG
    CTGAAGAATC CTATGGGCTC TGGCCCTCCA CTGTGTGTTG AAGATCTAGG ATTAAATTTC
    CAGTTCTGTC CATCATCCAA AGTACATGGT TTCTCTTCTG TAGACTTGAA GCCTAATGGA
    GAGGATGAGA TGGTCACCAT GGACAATGCA GAGGAGTATG TAGAGCTGAT GTTTGACTTC
    TGCATGCACA CAGGAATCCA GAAGCAAATG GAGGCCTTTA GAGAGGGCTT TAATAAAGTG
    TTCCCTATGG AGAAACTGAG CTCATTTAGC CATAAAGAGG TGCAGATGAT CCTGTGTGGA
    AACCAGTCTC CATCCTGGAC TGCTGAAGAC ATTGTTAACT ACACAGAACC TAAACTGGGC
    TATACACGGG ACAGTCCTGG TTTCCTGCGT TTCGTGCGGG TTCTGTGTGG AATGTCTTCT
    GATGAGAGGA AAGCTTTCCT CCAGTTCACT ACAGGCTGCT CAACTCTGCC CCCTGGTGGA
    CTGGCCAACC TCCATCCACG GCTCACAATA GTCCGAAAGG TTGATGCAAC AGATGCCAGC
    TATCCTTCAG TCAACACGTG TGTGCACTAC TTGAAGCTGC CGGAATATTC TTCAGAAGAG
    ATTATGAGAG AGCGCCTTCT CGCTGCCACT ATGGAGAAGG GTTTCCACCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47645
    Clone ID Related Accession (Same CDS sequence) XM_009293114.2 , XM_009293114.3
    Accession Version XM_009293114.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGACTT CTGTGAGCGT GGCTGCAGAT CTTCAGGCCT GAGTTCAGGG
    ACGTTGTCCA CGACGCAAAG CTGTGATATC TTGAGTGCTG CAAGAGAGCA GGCTCAGGCT
    AAAGCGGGCT CGGGTCAGAG CGCCTGCAGC GTAGAGGATG TACTGCAGCT CTTGCGCATC
    CTTTTCACCA TCGGAGGAGA ACCTACATCT GGACGCACAC TACAGGAAGA TGTGGAAGAG
    CTACAGTTCA ATGCATCACC TGAGGAATTC ACCAGCAAAA AAATTACAAC CAAAATCCTG
    CAGCAGATTG AAGAGCCATT GGCTCTGGCT AGTGGGGCTC TCCCCGACTG GTGTGAACAG
    TTAACCAGCA AGTGTCCTTT CCTCATACCC TTTGAAACCC GGCAGCTTTA TTTTACCTGC
    ACTGCATTTG GAGCCTCTAG GGCAATTGTT TGGCTCCAGA ATCGAAGGGA AGCCACTATG
    GAGCGTTCCC GGCCATCCAC GACTGTTCGC CGTGATGATC CCGGAGAGTT CCGTGTTGGT
    CGGCTTAAGC ATGAACGTGT CAAGGTGCCT CGAGGAGAGA GTATGATGGA GTGGGCTGAG
    AGTGTGATGC AGATACACGC TGACAGAAAG TCTGTACTGG AGGTGGAGTT CCAAGGGGAG
    GAGGGCACTG GTCTCGGACC CACCCTGGAG TTCTATGCTC TTGTTGCGGC AGAGTTTCAG
    AGGACCTCCC TTGGCATCTG GCTCTGTGAT GATGACTTCC CAGATGATGA GTCACGTCAG
    GTGGATCTGG GCGGTGGCTT GAAACCGCCA GGCTATTACG TGCAGCGTTC CTGCGGCCTT
    TTCCCTGCTC CCTTCCCTCA GGACAGCGAT GAACTGGAGC GTATTACCAA ACTTTTCTTG
    TTCCTTGGCA TCTTTCTGGC CAAATGCATC CAGGACAACC GGCTCGTGGA TCTGCCCATA
    TCCCAGCCTT TCTTTAAACT GCTCTGTATG GGTGACATCA AAAGCAACAT GAGTAAGCTA
    CTTTATCAAA CCCGTGGTGA GTCGGACTGT CACTTCTCTG AAATCCAGTC TGAGGCCTCC
    ACCGAGGAGG GTCAGGACAC GTACTCTGTG GGTAGTTTTG ATGAAGACTC CAAGTCTGAG
    TTTATTCTTG ACCCACCGAA GCCCAAACCA CCAGCCTGGT ATCATGGCAT CTTGACCTGG
    GAGGACTTTG AACTGGTAAA CCCTCACAGA GCACTGTTTC TTAAAGAGCT GAAGGCACTT
    TCTGTGAAGC GCAGGCAGAT TCTAGGCAAT AAGAGTCTGT CCGAGGATGA GAAGAACACA
    CGGCTTCAGG ATCTCATGCT GAAGAATCCT ATGGGCTCTG GCCCTCCACT GTGTGTTGAA
    GATCTAGGAT TAAATTTCCA GTTCTGTCCA TCATCCAAAG TACATGGTTT CTCTTCTGTA
    GACTTGAAGC CTAATGGAGA GGATGAGATG GTCACCATGG ACAATGCAGA GGAGTATGTA
    GAGCTGATGT TTGACTTCTG CATGCACACA GGAATCCAGA AGCAAATGGA GGCCTTTAGA
    GAGGGCTTTA ATAAAGTGTT CCCTATGGAG AAACTGAGCT CATTTAGCCA TAAAGAGGTG
    CAGATGATCC TGTGTGGAAA CCAGTCTCCA TCCTGGACTG CTGAAGACAT TGTTAACTAC
    ACAGAACCTA AACTGGGCTA TACACGGGAC AGTCCTGGTT TCCTGCGTTT CGTGCGGGTT
    CTGTGTGGAA TGTCTTCTGA TGAGAGGAAA GCTTTCCTCC AGTTCACTAC AGGCTGCTCA
    ACTCTGCCCC CTGGTGGACT GGCCAACCTC CATCCACGGC TCACAATAGT CCGAAAGGTT
    GATGCAACAG ATGCCAGCTA TCCTTCAGTC AACACGTGTG TGCACTACTT GAAGCTGCCG
    GAATATTCTT CAGAAGAGAT TATGAGAGAG CGCCTTCTCG CTGCCACTAT GGAGAAGGGT
    TTCCACCTCA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291389.1
    CDS463..8037
    Translation

    Target ORF information:

    RefSeq Version XM_009293114.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293114.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGACTT CTGTGAGCGT GGCTGCAGAT CTTCAGGCCT GAGTTCAGGG
    ACGTTGTCCA CGACGCAAAG CTGTGATATC TTGAGTGCTG CAAGAGAGCA GGCTCAGGCT
    AAAGCGGGCT CGGGTCAGAG CGCCTGCAGC GTAGAGGATG TACTGCAGCT CTTGCGCATC
    CTTTTCACCA TCGGAGGAGA ACCTACATCT GGACGCACAC TACAGGAAGA TGTGGAAGAG
    CTACAGTTCA ATGCATCACC TGAGGAATTC ACCAGCAAAA AAATTACAAC CAAAATCCTG
    CAGCAGATTG AAGAGCCATT GGCTCTGGCT AGTGGGGCTC TCCCCGACTG GTGTGAACAG
    TTAACCAGCA AGTGTCCTTT CCTCATACCC TTTGAAACCC GGCAGCTTTA TTTTACCTGC
    ACTGCATTTG GAGCCTCTAG GGCAATTGTT TGGCTCCAGA ATCGAAGGGA AGCCACTATG
    GAGCGTTCCC GGCCATCCAC GACTGTTCGC CGTGATGATC CCGGAGAGTT CCGTGTTGGT
    CGGCTTAAGC ATGAACGTGT CAAGGTGCCT CGAGGAGAGA GTATGATGGA GTGGGCTGAG
    AGTGTGATGC AGATACACGC TGACAGAAAG TCTGTACTGG AGGTGGAGTT CCAAGGGGAG
    GAGGGCACTG GTCTCGGACC CACCCTGGAG TTCTATGCTC TTGTTGCGGC AGAGTTTCAG
    AGGACCTCCC TTGGCATCTG GCTCTGTGAT GATGACTTCC CAGATGATGA GTCACGTCAG
    GTGGATCTGG GCGGTGGCTT GAAACCGCCA GGCTATTACG TGCAGCGTTC CTGCGGCCTT
    TTCCCTGCTC CCTTCCCTCA GGACAGCGAT GAACTGGAGC GTATTACCAA ACTTTTCTTG
    TTCCTTGGCA TCTTTCTGGC CAAATGCATC CAGGACAACC GGCTCGTGGA TCTGCCCATA
    TCCCAGCCTT TCTTTAAACT GCTCTGTATG GGTGACATCA AAAGCAACAT GAGTAAGCTA
    CTTTATCAAA CCCGTGGTGA GTCGGACTGT CACTTCTCTG AAATCCAGTC TGAGGCCTCC
    ACCGAGGAGG GTCAGGACAC GTACTCTGTG GGTAGTTTTG ATGAAGACTC CAAGTCTGAG
    TTTATTCTTG ACCCACCGAA GCCCAAACCA CCAGCCTGGT ATCATGGCAT CTTGACCTGG
    GAGGACTTTG AACTGGTAAA CCCTCACAGA GCACTGTTTC TTAAAGAGCT GAAGGCACTT
    TCTGTGAAGC GCAGGCAGAT TCTAGGCAAT AAGAGTCTGT CCGAGGATGA GAAGAACACA
    CGGCTTCAGG ATCTCATGCT GAAGAATCCT ATGGGCTCTG GCCCTCCACT GTGTGTTGAA
    GATCTAGGAT TAAATTTCCA GTTCTGTCCA TCATCCAAAG TACATGGTTT CTCTTCTGTA
    GACTTGAAGC CTAATGGAGA GGATGAGATG GTCACCATGG ACAATGCAGA GGAGTATGTA
    GAGCTGATGT TTGACTTCTG CATGCACACA GGAATCCAGA AGCAAATGGA GGCCTTTAGA
    GAGGGCTTTA ATAAAGTGTT CCCTATGGAG AAACTGAGCT CATTTAGCCA TAAAGAGGTG
    CAGATGATCC TGTGTGGAAA CCAGTCTCCA TCCTGGACTG CTGAAGACAT TGTTAACTAC
    ACAGAACCTA AACTGGGCTA TACACGGGAC AGTCCTGGTT TCCTGCGTTT CGTGCGGGTT
    CTGTGTGGAA TGTCTTCTGA TGAGAGGAAA GCTTTCCTCC AGTTCACTAC AGGCTGCTCA
    ACTCTGCCCC CTGGTGGACT GGCCAACCTC CATCCACGGC TCACAATAGT CCGAAAGGTT
    GATGCAACAG ATGCCAGCTA TCCTTCAGTC AACACGTGTG TGCACTACTT GAAGCTGCCG
    GAATATTCTT CAGAAGAGAT TATGAGAGAG CGCCTTCTCG CTGCCACTAT GGAGAAGGGT
    TTCCACCTCA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47645
    Clone ID Related Accession (Same CDS sequence) XM_009293114.2 , XM_009293114.3
    Accession Version XM_009293114.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2641
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    3001
    3061
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    3181
    3241
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    3421
    3481
    3541
    3601
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    3721
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    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGACTT CTGTGAGCGT GGCTGCAGAT CTTCAGGCCT GAGTTCAGGG
    ACGTTGTCCA CGACGCAAAG CTGTGATATC TTGAGTGCTG CAAGAGAGCA GGCTCAGGCT
    AAAGCGGGCT CGGGTCAGAG CGCCTGCAGC GTAGAGGATG TACTGCAGCT CTTGCGCATC
    CTTTTCACCA TCGGAGGAGA ACCTACATCT GGACGCACAC TACAGGAAGA TGTGGAAGAG
    CTACAGTTCA ATGCATCACC TGAGGAATTC ACCAGCAAAA AAATTACAAC CAAAATCCTG
    CAGCAGATTG AAGAGCCATT GGCTCTGGCT AGTGGGGCTC TCCCCGACTG GTGTGAACAG
    TTAACCAGCA AGTGTCCTTT CCTCATACCC TTTGAAACCC GGCAGCTTTA TTTTACCTGC
    ACTGCATTTG GAGCCTCTAG GGCAATTGTT TGGCTCCAGA ATCGAAGGGA AGCCACTATG
    GAGCGTTCCC GGCCATCCAC GACTGTTCGC CGTGATGATC CCGGAGAGTT CCGTGTTGGT
    CGGCTTAAGC ATGAACGTGT CAAGGTGCCT CGAGGAGAGA GTATGATGGA GTGGGCTGAG
    AGTGTGATGC AGATACACGC TGACAGAAAG TCTGTACTGG AGGTGGAGTT CCAAGGGGAG
    GAGGGCACTG GTCTCGGACC CACCCTGGAG TTCTATGCTC TTGTTGCGGC AGAGTTTCAG
    AGGACCTCCC TTGGCATCTG GCTCTGTGAT GATGACTTCC CAGATGATGA GTCACGTCAG
    GTGGATCTGG GCGGTGGCTT GAAACCGCCA GGCTATTACG TGCAGCGTTC CTGCGGCCTT
    TTCCCTGCTC CCTTCCCTCA GGACAGCGAT GAACTGGAGC GTATTACCAA ACTTTTCTTG
    TTCCTTGGCA TCTTTCTGGC CAAATGCATC CAGGACAACC GGCTCGTGGA TCTGCCCATA
    TCCCAGCCTT TCTTTAAACT GCTCTGTATG GGTGACATCA AAAGCAACAT GAGTAAGCTA
    CTTTATCAAA CCCGTGGTGA GTCGGACTGT CACTTCTCTG AAATCCAGTC TGAGGCCTCC
    ACCGAGGAGG GTCAGGACAC GTACTCTGTG GGTAGTTTTG ATGAAGACTC CAAGTCTGAG
    TTTATTCTTG ACCCACCGAA GCCCAAACCA CCAGCCTGGT ATCATGGCAT CTTGACCTGG
    GAGGACTTTG AACTGGTAAA CCCTCACAGA GCACTGTTTC TTAAAGAGCT GAAGGCACTT
    TCTGTGAAGC GCAGGCAGAT TCTAGGCAAT AAGAGTCTGT CCGAGGATGA GAAGAACACA
    CGGCTTCAGG ATCTCATGCT GAAGAATCCT ATGGGCTCTG GCCCTCCACT GTGTGTTGAA
    GATCTAGGAT TAAATTTCCA GTTCTGTCCA TCATCCAAAG TACATGGTTT CTCTTCTGTA
    GACTTGAAGC CTAATGGAGA GGATGAGATG GTCACCATGG ACAATGCAGA GGAGTATGTA
    GAGCTGATGT TTGACTTCTG CATGCACACA GGAATCCAGA AGCAAATGGA GGCCTTTAGA
    GAGGGCTTTA ATAAAGTGTT CCCTATGGAG AAACTGAGCT CATTTAGCCA TAAAGAGGTG
    CAGATGATCC TGTGTGGAAA CCAGTCTCCA TCCTGGACTG CTGAAGACAT TGTTAACTAC
    ACAGAACCTA AACTGGGCTA TACACGGGAC AGTCCTGGTT TCCTGCGTTT CGTGCGGGTT
    CTGTGTGGAA TGTCTTCTGA TGAGAGGAAA GCTTTCCTCC AGTTCACTAC AGGCTGCTCA
    ACTCTGCCCC CTGGTGGACT GGCCAACCTC CATCCACGGC TCACAATAGT CCGAAAGGTT
    GATGCAACAG ATGCCAGCTA TCCTTCAGTC AACACGTGTG TGCACTACTT GAAGCTGCCG
    GAATATTCTT CAGAAGAGAT TATGAGAGAG CGCCTTCTCG CTGCCACTAT GGAGAAGGGT
    TTCCACCTCA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291389.1
    CDS463..8037
    Translation

    Target ORF information:

    RefSeq Version XM_009293114.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGTACG GTCCAGGGTC AACCTTGGTT ACCACGTTCA TATTCATAGG
    GAGGAAGAGG AGTATGAAAC CAAAGGGGGC CGTCGCAGGA CCTGGGATGA CGACTTTGTG
    CTGAAACGAC AGTTTTCTGC TTTAGTACCT GCATTTGACC CTAGACCAGG CAGGACCAAC
    GTCCAGCAAA CTACTGACCT GGAAATCCCC CCACCAGGAA CACCTCGTTC AGAGGTACAG
    GAGGAGGTGG AGTGCGCTCC TTCACCTCGT CTGGCTCTCA TCTTAAAAGT AGCAGGTCTA
    GGAACTACTC GAGAAGTAGA ACTACCTCTC ACCAATTACA AGTCCACCAT TTTCTATTAT
    GTCCAAAAAC TTCTCCAGCT GTCATGCAAC GGTGCTATTA AACCTGACAA GCTCAGACGC
    ATCTGGGAGC CTACGTATAC GATAATGTAT AGAGAATTGA AGGATTCCGA CAAAGAAAGA
    GAGAGCGGAA AGATGGACTT CTGTGAGCGT GGCTGCAGAT CTTCAGGCCT GAGTTCAGGG
    ACGTTGTCCA CGACGCAAAG CTGTGATATC TTGAGTGCTG CAAGAGAGCA GGCTCAGGCT
    AAAGCGGGCT CGGGTCAGAG CGCCTGCAGC GTAGAGGATG TACTGCAGCT CTTGCGCATC
    CTTTTCACCA TCGGAGGAGA ACCTACATCT GGACGCACAC TACAGGAAGA TGTGGAAGAG
    CTACAGTTCA ATGCATCACC TGAGGAATTC ACCAGCAAAA AAATTACAAC CAAAATCCTG
    CAGCAGATTG AAGAGCCATT GGCTCTGGCT AGTGGGGCTC TCCCCGACTG GTGTGAACAG
    TTAACCAGCA AGTGTCCTTT CCTCATACCC TTTGAAACCC GGCAGCTTTA TTTTACCTGC
    ACTGCATTTG GAGCCTCTAG GGCAATTGTT TGGCTCCAGA ATCGAAGGGA AGCCACTATG
    GAGCGTTCCC GGCCATCCAC GACTGTTCGC CGTGATGATC CCGGAGAGTT CCGTGTTGGT
    CGGCTTAAGC ATGAACGTGT CAAGGTGCCT CGAGGAGAGA GTATGATGGA GTGGGCTGAG
    AGTGTGATGC AGATACACGC TGACAGAAAG TCTGTACTGG AGGTGGAGTT CCAAGGGGAG
    GAGGGCACTG GTCTCGGACC CACCCTGGAG TTCTATGCTC TTGTTGCGGC AGAGTTTCAG
    AGGACCTCCC TTGGCATCTG GCTCTGTGAT GATGACTTCC CAGATGATGA GTCACGTCAG
    GTGGATCTGG GCGGTGGCTT GAAACCGCCA GGCTATTACG TGCAGCGTTC CTGCGGCCTT
    TTCCCTGCTC CCTTCCCTCA GGACAGCGAT GAACTGGAGC GTATTACCAA ACTTTTCTTG
    TTCCTTGGCA TCTTTCTGGC CAAATGCATC CAGGACAACC GGCTCGTGGA TCTGCCCATA
    TCCCAGCCTT TCTTTAAACT GCTCTGTATG GGTGACATCA AAAGCAACAT GAGTAAGCTA
    CTTTATCAAA CCCGTGGTGA GTCGGACTGT CACTTCTCTG AAATCCAGTC TGAGGCCTCC
    ACCGAGGAGG GTCAGGACAC GTACTCTGTG GGTAGTTTTG ATGAAGACTC CAAGTCTGAG
    TTTATTCTTG ACCCACCGAA GCCCAAACCA CCAGCCTGGT ATCATGGCAT CTTGACCTGG
    GAGGACTTTG AACTGGTAAA CCCTCACAGA GCACTGTTTC TTAAAGAGCT GAAGGCACTT
    TCTGTGAAGC GCAGGCAGAT TCTAGGCAAT AAGAGTCTGT CCGAGGATGA GAAGAACACA
    CGGCTTCAGG ATCTCATGCT GAAGAATCCT ATGGGCTCTG GCCCTCCACT GTGTGTTGAA
    GATCTAGGAT TAAATTTCCA GTTCTGTCCA TCATCCAAAG TACATGGTTT CTCTTCTGTA
    GACTTGAAGC CTAATGGAGA GGATGAGATG GTCACCATGG ACAATGCAGA GGAGTATGTA
    GAGCTGATGT TTGACTTCTG CATGCACACA GGAATCCAGA AGCAAATGGA GGCCTTTAGA
    GAGGGCTTTA ATAAAGTGTT CCCTATGGAG AAACTGAGCT CATTTAGCCA TAAAGAGGTG
    CAGATGATCC TGTGTGGAAA CCAGTCTCCA TCCTGGACTG CTGAAGACAT TGTTAACTAC
    ACAGAACCTA AACTGGGCTA TACACGGGAC AGTCCTGGTT TCCTGCGTTT CGTGCGGGTT
    CTGTGTGGAA TGTCTTCTGA TGAGAGGAAA GCTTTCCTCC AGTTCACTAC AGGCTGCTCA
    ACTCTGCCCC CTGGTGGACT GGCCAACCTC CATCCACGGC TCACAATAGT CCGAAAGGTT
    GATGCAACAG ATGCCAGCTA TCCTTCAGTC AACACGTGTG TGCACTACTT GAAGCTGCCG
    GAATATTCTT CAGAAGAGAT TATGAGAGAG CGCCTTCTCG CTGCCACTAT GGAGAAGGGT
    TTCCACCTCA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47646
    Clone ID Related Accession (Same CDS sequence) XM_009293115.2 , XM_009293115.3
    Accession Version XM_009293115.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GACTTCTGTG AGCGTGGCTG CAGATCTTCA
    GGCCTGAGTT CAGGGACGTT GTCCACGACG CAAAGCTGTG ATATCTTGAG TGCTGCAAGA
    GAGCAGGCTC AGGCTAAAGC GGGCTCGGGT CAGAGCGCCT GCAGCGTAGA GGATGTACTG
    CAGCTCTTGC GCATCCTTTT CACCATCGGA GGAGAACCTA CATCTGGACG CACACTACAG
    GAAGATGTGG AAGAGCTACA GTTCAATGCA TCACCTGAGG AATTCACCAG CAAAAAAATT
    ACAACCAAAA TCCTGCAGCA GATTGAAGAG CCATTGGCTC TGGCTAGTGG GGCTCTCCCC
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTCA TACCCTTTGA AACCCGGCAG
    CTTTATTTTA CCTGCACTGC ATTTGGAGCC TCTAGGGCAA TTGTTTGGCT CCAGAATCGA
    AGGGAAGCCA CTATGGAGCG TTCCCGGCCA TCCACGACTG TTCGCCGTGA TGATCCCGGA
    GAGTTCCGTG TTGGTCGGCT TAAGCATGAA CGTGTCAAGG TGCCTCGAGG AGAGAGTATG
    ATGGAGTGGG CTGAGAGTGT GATGCAGATA CACGCTGACA GAAAGTCTGT ACTGGAGGTG
    GAGTTCCAAG GGGAGGAGGG CACTGGTCTC GGACCCACCC TGGAGTTCTA TGCTCTTGTT
    GCGGCAGAGT TTCAGAGGAC CTCCCTTGGC ATCTGGCTCT GTGATGATGA CTTCCCAGAT
    GATGAGTCAC GTCAGGTGGA TCTGGGCGGT GGCTTGAAAC CGCCAGGCTA TTACGTGCAG
    CGTTCCTGCG GCCTTTTCCC TGCTCCCTTC CCTCAGGACA GCGATGAACT GGAGCGTATT
    ACCAAACTTT TCTTGTTCCT TGGCATCTTT CTGGCCAAAT GCATCCAGGA CAACCGGCTC
    GTGGATCTGC CCATATCCCA GCCTTTCTTT AAACTGCTCT GTATGGGTGA CATCAAAAGC
    AACATGAGTA AGCTACTTTA TCAAACCCGT GGTGAGTCGG ACTGTCACTT CTCTGAAATC
    CAGTCTGAGG CCTCCACCGA GGAGGGTCAG GACACGTACT CTGTGGGTAG TTTTGATGAA
    GACTCCAAGT CTGAGTTTAT TCTTGACCCA CCGAAGCCCA AACCACCAGC CTGGTATCAT
    GGCATCTTGA CCTGGGAGGA CTTTGAACTG GTAAACCCTC ACAGAGCACT GTTTCTTAAA
    GAGCTGAAGG CACTTTCTGT GAAGCGCAGG CAGATTCTAG GCAATAAGAG TCTGTCCGAG
    GATGAGAAGA ACACACGGCT TCAGGATCTC ATGCTGAAGA ATCCTATGGG CTCTGGCCCT
    CCACTGTGTG TTGAAGATCT AGGATTAAAT TTCCAGTTCT GTCCATCATC CAAAGTACAT
    GGTTTCTCTT CTGTAGACTT GAAGCCTAAT GGAGAGGATG AGATGGTCAC CATGGACAAT
    GCAGAGGAGT ATGTAGAGCT GATGTTTGAC TTCTGCATGC ACACAGGAAT CCAGAAGCAA
    ATGGAGGCCT TTAGAGAGGG CTTTAATAAA GTGTTCCCTA TGGAGAAACT GAGCTCATTT
    AGCCATAAAG AGGTGCAGAT GATCCTGTGT GGAAACCAGT CTCCATCCTG GACTGCTGAA
    GACATTGTTA ACTACACAGA ACCTAAACTG GGCTATACAC GGGACAGTCC TGGTTTCCTG
    CGTTTCGTGC GGGTTCTGTG TGGAATGTCT TCTGATGAGA GGAAAGCTTT CCTCCAGTTC
    ACTACAGGCT GCTCAACTCT GCCCCCTGGT GGACTGGCCA ACCTCCATCC ACGGCTCACA
    ATAGTCCGAA AGGTTGATGC AACAGATGCC AGCTATCCTT CAGTCAACAC GTGTGTGCAC
    TACTTGAAGC TGCCGGAATA TTCTTCAGAA GAGATTATGA GAGAGCGCCT TCTCGCTGCC
    ACTATGGAGA AGGGTTTCCA CCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291390.1
    CDS463..7992
    Translation

    Target ORF information:

    RefSeq Version XM_009293115.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293115.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GACTTCTGTG AGCGTGGCTG CAGATCTTCA
    GGCCTGAGTT CAGGGACGTT GTCCACGACG CAAAGCTGTG ATATCTTGAG TGCTGCAAGA
    GAGCAGGCTC AGGCTAAAGC GGGCTCGGGT CAGAGCGCCT GCAGCGTAGA GGATGTACTG
    CAGCTCTTGC GCATCCTTTT CACCATCGGA GGAGAACCTA CATCTGGACG CACACTACAG
    GAAGATGTGG AAGAGCTACA GTTCAATGCA TCACCTGAGG AATTCACCAG CAAAAAAATT
    ACAACCAAAA TCCTGCAGCA GATTGAAGAG CCATTGGCTC TGGCTAGTGG GGCTCTCCCC
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTCA TACCCTTTGA AACCCGGCAG
    CTTTATTTTA CCTGCACTGC ATTTGGAGCC TCTAGGGCAA TTGTTTGGCT CCAGAATCGA
    AGGGAAGCCA CTATGGAGCG TTCCCGGCCA TCCACGACTG TTCGCCGTGA TGATCCCGGA
    GAGTTCCGTG TTGGTCGGCT TAAGCATGAA CGTGTCAAGG TGCCTCGAGG AGAGAGTATG
    ATGGAGTGGG CTGAGAGTGT GATGCAGATA CACGCTGACA GAAAGTCTGT ACTGGAGGTG
    GAGTTCCAAG GGGAGGAGGG CACTGGTCTC GGACCCACCC TGGAGTTCTA TGCTCTTGTT
    GCGGCAGAGT TTCAGAGGAC CTCCCTTGGC ATCTGGCTCT GTGATGATGA CTTCCCAGAT
    GATGAGTCAC GTCAGGTGGA TCTGGGCGGT GGCTTGAAAC CGCCAGGCTA TTACGTGCAG
    CGTTCCTGCG GCCTTTTCCC TGCTCCCTTC CCTCAGGACA GCGATGAACT GGAGCGTATT
    ACCAAACTTT TCTTGTTCCT TGGCATCTTT CTGGCCAAAT GCATCCAGGA CAACCGGCTC
    GTGGATCTGC CCATATCCCA GCCTTTCTTT AAACTGCTCT GTATGGGTGA CATCAAAAGC
    AACATGAGTA AGCTACTTTA TCAAACCCGT GGTGAGTCGG ACTGTCACTT CTCTGAAATC
    CAGTCTGAGG CCTCCACCGA GGAGGGTCAG GACACGTACT CTGTGGGTAG TTTTGATGAA
    GACTCCAAGT CTGAGTTTAT TCTTGACCCA CCGAAGCCCA AACCACCAGC CTGGTATCAT
    GGCATCTTGA CCTGGGAGGA CTTTGAACTG GTAAACCCTC ACAGAGCACT GTTTCTTAAA
    GAGCTGAAGG CACTTTCTGT GAAGCGCAGG CAGATTCTAG GCAATAAGAG TCTGTCCGAG
    GATGAGAAGA ACACACGGCT TCAGGATCTC ATGCTGAAGA ATCCTATGGG CTCTGGCCCT
    CCACTGTGTG TTGAAGATCT AGGATTAAAT TTCCAGTTCT GTCCATCATC CAAAGTACAT
    GGTTTCTCTT CTGTAGACTT GAAGCCTAAT GGAGAGGATG AGATGGTCAC CATGGACAAT
    GCAGAGGAGT ATGTAGAGCT GATGTTTGAC TTCTGCATGC ACACAGGAAT CCAGAAGCAA
    ATGGAGGCCT TTAGAGAGGG CTTTAATAAA GTGTTCCCTA TGGAGAAACT GAGCTCATTT
    AGCCATAAAG AGGTGCAGAT GATCCTGTGT GGAAACCAGT CTCCATCCTG GACTGCTGAA
    GACATTGTTA ACTACACAGA ACCTAAACTG GGCTATACAC GGGACAGTCC TGGTTTCCTG
    CGTTTCGTGC GGGTTCTGTG TGGAATGTCT TCTGATGAGA GGAAAGCTTT CCTCCAGTTC
    ACTACAGGCT GCTCAACTCT GCCCCCTGGT GGACTGGCCA ACCTCCATCC ACGGCTCACA
    ATAGTCCGAA AGGTTGATGC AACAGATGCC AGCTATCCTT CAGTCAACAC GTGTGTGCAC
    TACTTGAAGC TGCCGGAATA TTCTTCAGAA GAGATTATGA GAGAGCGCCT TCTCGCTGCC
    ACTATGGAGA AGGGTTTCCA CCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47646
    Clone ID Related Accession (Same CDS sequence) XM_009293115.2 , XM_009293115.3
    Accession Version XM_009293115.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GACTTCTGTG AGCGTGGCTG CAGATCTTCA
    GGCCTGAGTT CAGGGACGTT GTCCACGACG CAAAGCTGTG ATATCTTGAG TGCTGCAAGA
    GAGCAGGCTC AGGCTAAAGC GGGCTCGGGT CAGAGCGCCT GCAGCGTAGA GGATGTACTG
    CAGCTCTTGC GCATCCTTTT CACCATCGGA GGAGAACCTA CATCTGGACG CACACTACAG
    GAAGATGTGG AAGAGCTACA GTTCAATGCA TCACCTGAGG AATTCACCAG CAAAAAAATT
    ACAACCAAAA TCCTGCAGCA GATTGAAGAG CCATTGGCTC TGGCTAGTGG GGCTCTCCCC
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTCA TACCCTTTGA AACCCGGCAG
    CTTTATTTTA CCTGCACTGC ATTTGGAGCC TCTAGGGCAA TTGTTTGGCT CCAGAATCGA
    AGGGAAGCCA CTATGGAGCG TTCCCGGCCA TCCACGACTG TTCGCCGTGA TGATCCCGGA
    GAGTTCCGTG TTGGTCGGCT TAAGCATGAA CGTGTCAAGG TGCCTCGAGG AGAGAGTATG
    ATGGAGTGGG CTGAGAGTGT GATGCAGATA CACGCTGACA GAAAGTCTGT ACTGGAGGTG
    GAGTTCCAAG GGGAGGAGGG CACTGGTCTC GGACCCACCC TGGAGTTCTA TGCTCTTGTT
    GCGGCAGAGT TTCAGAGGAC CTCCCTTGGC ATCTGGCTCT GTGATGATGA CTTCCCAGAT
    GATGAGTCAC GTCAGGTGGA TCTGGGCGGT GGCTTGAAAC CGCCAGGCTA TTACGTGCAG
    CGTTCCTGCG GCCTTTTCCC TGCTCCCTTC CCTCAGGACA GCGATGAACT GGAGCGTATT
    ACCAAACTTT TCTTGTTCCT TGGCATCTTT CTGGCCAAAT GCATCCAGGA CAACCGGCTC
    GTGGATCTGC CCATATCCCA GCCTTTCTTT AAACTGCTCT GTATGGGTGA CATCAAAAGC
    AACATGAGTA AGCTACTTTA TCAAACCCGT GGTGAGTCGG ACTGTCACTT CTCTGAAATC
    CAGTCTGAGG CCTCCACCGA GGAGGGTCAG GACACGTACT CTGTGGGTAG TTTTGATGAA
    GACTCCAAGT CTGAGTTTAT TCTTGACCCA CCGAAGCCCA AACCACCAGC CTGGTATCAT
    GGCATCTTGA CCTGGGAGGA CTTTGAACTG GTAAACCCTC ACAGAGCACT GTTTCTTAAA
    GAGCTGAAGG CACTTTCTGT GAAGCGCAGG CAGATTCTAG GCAATAAGAG TCTGTCCGAG
    GATGAGAAGA ACACACGGCT TCAGGATCTC ATGCTGAAGA ATCCTATGGG CTCTGGCCCT
    CCACTGTGTG TTGAAGATCT AGGATTAAAT TTCCAGTTCT GTCCATCATC CAAAGTACAT
    GGTTTCTCTT CTGTAGACTT GAAGCCTAAT GGAGAGGATG AGATGGTCAC CATGGACAAT
    GCAGAGGAGT ATGTAGAGCT GATGTTTGAC TTCTGCATGC ACACAGGAAT CCAGAAGCAA
    ATGGAGGCCT TTAGAGAGGG CTTTAATAAA GTGTTCCCTA TGGAGAAACT GAGCTCATTT
    AGCCATAAAG AGGTGCAGAT GATCCTGTGT GGAAACCAGT CTCCATCCTG GACTGCTGAA
    GACATTGTTA ACTACACAGA ACCTAAACTG GGCTATACAC GGGACAGTCC TGGTTTCCTG
    CGTTTCGTGC GGGTTCTGTG TGGAATGTCT TCTGATGAGA GGAAAGCTTT CCTCCAGTTC
    ACTACAGGCT GCTCAACTCT GCCCCCTGGT GGACTGGCCA ACCTCCATCC ACGGCTCACA
    ATAGTCCGAA AGGTTGATGC AACAGATGCC AGCTATCCTT CAGTCAACAC GTGTGTGCAC
    TACTTGAAGC TGCCGGAATA TTCTTCAGAA GAGATTATGA GAGAGCGCCT TCTCGCTGCC
    ACTATGGAGA AGGGTTTCCA CCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291390.1
    CDS463..7992
    Translation

    Target ORF information:

    RefSeq Version XM_009293115.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293115.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCTGGATT GATGTCACCT GGGATGCCGG TGGCTCAAAC
    TCATATCGTA TGGGTGCGGA AGGGAAGTTT GACCTCAAGC TTGCGCCAGG GTACGACCCT
    GAGTCAGCGC CATCACCCAA ACCTGTCTCA TCCACTGTTG CAGGCACACC GCAGTCCTGG
    AGCAGCCTGG TGAAAAATAA CTGTCCAGAC AAGGGTGGCT CATCCTCCAC AGCGGGGGCC
    AGTTCCTCCA GCCGCAAGGG TAGTAGCAGT TCCGTGTGTA GCGTGGCCAG CAGCAGCGAT
    ATAAGCCTCA GCTCCACCCG AGTGGAGCGC AGGGTCGAGA GCCTGTTCGA GCAGGGAGTA
    GGCGTCATCG GAGGGCCCCC AGGAGCTGAG GGCCAAGAAC CCATAGTTGT GCTTTCCTCA
    GCCGAAGCTG GCTCCGCCTC CAGCACCAGC ACATTGACAG CAGACACAGG AAGCGAAAGC
    GAGCGTAAAA CACCAGGACC TGATGGCACT AGACAGTCAG CGGAGTCCAC AGCCATCTCT
    ATGGGAATCG TGAGCGTGAG CTCGCCTGAT GTCAGCTCTG TTTCAGAGTC GTCTAGTAAA
    GACGCTGCAT CCCAGAGGCC CTTGTGCTCT GCCACCAGTG CGCGGCTGTC TGTCAGTTCA
    CTTTTGGCAG CTGGAGCACC CATGAGTTCT AGTGCCAGCG TCCCCAACCT GTCCTCTCGC
    GAGGCCAGCC TGATGGAGTC GTTCGTTCGG AGGGCACCCA ACATGTCTCG CACTAATGCC
    ACCAACAACA TGAACCTGAG CCGAAGCAGC AGTGATAACA ACACTAACAC GCTGGGACGA
    AATGTCATGA GCACTGCCAC TTCTCCACTT ATGGGTGCGC AGAGCTTTCC TAACCTTACA
    ACCACTGGTA CCACCTCAAC TGTTACAATG TCCACCTCTA TAGTAACCAG CAGCAATAAT
    GTAGCCACGG CAACTACAGG TTTGTCCGTC GGCCAGTTGC TCAGTAACAC GCTGACGACC
    AGCCTGACCT CTACATCTAG CGAGAGTGAC ACAGGTCAGG AGGCTGAATT TTCCCTCTAT
    GACTTCCTGG ACAGTTGTCG GGCCAACACT TTGCTTGCAG AGTTGGATGA TGAAGAAGAT
    TTGCCGGAGC CAGATGATGA CGATGATGAA AATGAGGATG ACAATCAGGA GGAACAGGAG
    TATGAGGAAG TTTTGGAGGA AGAGGAGTAT GAAACCAAAG GGGGCCGTCG CAGGACCTGG
    GATGACGACT TTGTGCTGAA ACGACAGTTT TCTGCTTTAG TACCTGCATT TGACCCTAGA
    CCAGGCAGGA CCAACGTCCA GCAAACTACT GACCTGGAAA TCCCCCCACC AGGAACACCT
    CGTTCAGAGG TACAGGAGGA GGTGGAGTGC GCTCCTTCAC CTCGTCTGGC TCTCATCTTA
    AAAGTAGCAG GTCTAGGAAC TACTCGAGAA GTAGAACTAC CTCTCACCAA TTACAAGTCC
    ACCATTTTCT ATTATGTCCA AAAACTTCTC CAGCTGTCAT GCAACGGTGC TATTAAACCT
    GACAAGCTCA GACGCATCTG GGAGCCTACG TATACGATAA TGTATAGAGA ATTGAAGGAT
    TCCGACAAAG AAAGAGAGAG CGGAAAGATG GACTTCTGTG AGCGTGGCTG CAGATCTTCA
    GGCCTGAGTT CAGGGACGTT GTCCACGACG CAAAGCTGTG ATATCTTGAG TGCTGCAAGA
    GAGCAGGCTC AGGCTAAAGC GGGCTCGGGT CAGAGCGCCT GCAGCGTAGA GGATGTACTG
    CAGCTCTTGC GCATCCTTTT CACCATCGGA GGAGAACCTA CATCTGGACG CACACTACAG
    GAAGATGTGG AAGAGCTACA GTTCAATGCA TCACCTGAGG AATTCACCAG CAAAAAAATT
    ACAACCAAAA TCCTGCAGCA GATTGAAGAG CCATTGGCTC TGGCTAGTGG GGCTCTCCCC
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTCA TACCCTTTGA AACCCGGCAG
    CTTTATTTTA CCTGCACTGC ATTTGGAGCC TCTAGGGCAA TTGTTTGGCT CCAGAATCGA
    AGGGAAGCCA CTATGGAGCG TTCCCGGCCA TCCACGACTG TTCGCCGTGA TGATCCCGGA
    GAGTTCCGTG TTGGTCGGCT TAAGCATGAA CGTGTCAAGG TGCCTCGAGG AGAGAGTATG
    ATGGAGTGGG CTGAGAGTGT GATGCAGATA CACGCTGACA GAAAGTCTGT ACTGGAGGTG
    GAGTTCCAAG GGGAGGAGGG CACTGGTCTC GGACCCACCC TGGAGTTCTA TGCTCTTGTT
    GCGGCAGAGT TTCAGAGGAC CTCCCTTGGC ATCTGGCTCT GTGATGATGA CTTCCCAGAT
    GATGAGTCAC GTCAGGTGGA TCTGGGCGGT GGCTTGAAAC CGCCAGGCTA TTACGTGCAG
    CGTTCCTGCG GCCTTTTCCC TGCTCCCTTC CCTCAGGACA GCGATGAACT GGAGCGTATT
    ACCAAACTTT TCTTGTTCCT TGGCATCTTT CTGGCCAAAT GCATCCAGGA CAACCGGCTC
    GTGGATCTGC CCATATCCCA GCCTTTCTTT AAACTGCTCT GTATGGGTGA CATCAAAAGC
    AACATGAGTA AGCTACTTTA TCAAACCCGT GGTGAGTCGG ACTGTCACTT CTCTGAAATC
    CAGTCTGAGG CCTCCACCGA GGAGGGTCAG GACACGTACT CTGTGGGTAG TTTTGATGAA
    GACTCCAAGT CTGAGTTTAT TCTTGACCCA CCGAAGCCCA AACCACCAGC CTGGTATCAT
    GGCATCTTGA CCTGGGAGGA CTTTGAACTG GTAAACCCTC ACAGAGCACT GTTTCTTAAA
    GAGCTGAAGG CACTTTCTGT GAAGCGCAGG CAGATTCTAG GCAATAAGAG TCTGTCCGAG
    GATGAGAAGA ACACACGGCT TCAGGATCTC ATGCTGAAGA ATCCTATGGG CTCTGGCCCT
    CCACTGTGTG TTGAAGATCT AGGATTAAAT TTCCAGTTCT GTCCATCATC CAAAGTACAT
    GGTTTCTCTT CTGTAGACTT GAAGCCTAAT GGAGAGGATG AGATGGTCAC CATGGACAAT
    GCAGAGGAGT ATGTAGAGCT GATGTTTGAC TTCTGCATGC ACACAGGAAT CCAGAAGCAA
    ATGGAGGCCT TTAGAGAGGG CTTTAATAAA GTGTTCCCTA TGGAGAAACT GAGCTCATTT
    AGCCATAAAG AGGTGCAGAT GATCCTGTGT GGAAACCAGT CTCCATCCTG GACTGCTGAA
    GACATTGTTA ACTACACAGA ACCTAAACTG GGCTATACAC GGGACAGTCC TGGTTTCCTG
    CGTTTCGTGC GGGTTCTGTG TGGAATGTCT TCTGATGAGA GGAAAGCTTT CCTCCAGTTC
    ACTACAGGCT GCTCAACTCT GCCCCCTGGT GGACTGGCCA ACCTCCATCC ACGGCTCACA
    ATAGTCCGAA AGGTTGATGC AACAGATGCC AGCTATCCTT CAGTCAACAC GTGTGTGCAC
    TACTTGAAGC TGCCGGAATA TTCTTCAGAA GAGATTATGA GAGAGCGCCT TCTCGCTGCC
    ACTATGGAGA AGGGTTTCCA CCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47648
    Clone ID Related Accession (Same CDS sequence) XM_009293117.2 , XM_009293117.3
    Accession Version XM_009293117.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGAGGAA
    GAGGAGTATG AAACCAAAGG GGGCCGTCGC AGGACCTGGG ATGACGACTT TGTGCTGAAA
    CGACAGTTTT CTGCTTTAGT ACCTGCATTT GACCCTAGAC CAGGCAGGAC CAACGTCCAG
    CAAACTACTG ACCTGGAAAT CCCCCCACCA GGAACACCTC GTTCAGAGGT ACAGGAGGAG
    GTGGAGTGCG CTCCTTCACC TCGTCTGGCT CTCATCTTAA AAGTAGCAGG TCTAGGAACT
    ACTCGAGAAG TAGAACTACC TCTCACCAAT TACAAGTCCA CCATTTTCTA TTATGTCCAA
    AAACTTCTCC AGCTGTCATG CAACGGTGCT ATTAAACCTG ACAAGCTCAG ACGCATCTGG
    GAGCCTACGT ATACGATAAT GTATAGAGAA TTGAAGGATT CCGACAAAGA AAGAGAGAGC
    GGAAAGATGG GTTGCTGGTC TGTAGAGCAT GTGGAACAGT ACCTTGGCAC AGATGAATTA
    CCAAAGAATG ACTTGATAAC CTACATGCAG AAGAATGCAG ACTCAACTTT CCTGCGCCAC
    TGGAAATTAA CCGGCTCTAA TAAAAGTATT AGGAAAAACA GAAATTGTTC GCAGCTCATA
    GCTGCATACA AGGACTTCTG TGAGCGTGGC TGCAGATCTT CAGGCCTGAG TTCAGGGACG
    TTGTCCACGA CGCAAAGCTG TGATATCTTG AGTGCTGCAA GAGAGCAGGC TCAGGCTAAA
    GCGGGCTCGG GTCAGAGCGC CTGCAGCGTA GAGGATGTAC TGCAGCTCTT GCGCATCCTT
    TTCACCATCG GAGGAGAACC TACATCTGGA CGCACACTAC AGGAAGATGT GGAAGAGCTA
    CAGTTCAATG CATCACCTGA GGAATTCACC AGCAAAAAAA TTACAACCAA AATCCTGCAG
    CAGATTGAAG AGCCATTGGC TCTGGCTAGT GGGGCTCTCC CCGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT CATACCCTTT GAAACCCGGC AGCTTTATTT TACCTGCACT
    GCATTTGGAG CCTCTAGGGC AATTGTTTGG CTCCAGAATC GAAGGGAAGC CACTATGGAG
    CGTTCCCGGC CATCCACGAC TGTTCGCCGT GATGATCCCG GAGAGTTCCG TGTTGGTCGG
    CTTAAGCATG AACGTGTCAA GGTGCCTCGA GGAGAGAGTA TGATGGAGTG GGCTGAGAGT
    GTGATGCAGA TACACGCTGA CAGAAAGTCT GTACTGGAGG TGGAGTTCCA AGGGGAGGAG
    GGCACTGGTC TCGGACCCAC CCTGGAGTTC TATGCTCTTG TTGCGGCAGA GTTTCAGAGG
    ACCTCCCTTG GCATCTGGCT CTGTGATGAT GACTTCCCAG ATGATGAGTC ACGTCAGGTG
    GATCTGGGCG GTGGCTTGAA ACCGCCAGGC TATTACGTGC AGCGTTCCTG CGGCCTTTTC
    CCTGCTCCCT TCCCTCAGGA CAGCGATGAA CTGGAGCGTA TTACCAAACT TTTCTTGTTC
    CTTGGCATCT TTCTGGCCAA ATGCATCCAG GACAACCGGC TCGTGGATCT GCCCATATCC
    CAGCCTTTCT TTAAACTGCT CTGTATGGGT GACATCAAAA GCAACATGAG TAAGCTACTT
    TATCAAACCC GTGGTGAGTC GGACTGTCAC TTCTCTGAAA TCCAGTCTGA GGCCTCCACC
    GAGGAGGGTC AGGACACGTA CTCTGTGGGT AGTTTTGATG AAGACTCCAA GTCTGAGTTT
    ATTCTTGACC CACCGAAGCC CAAACCACCA GCCTGGTATC ATGGCATCTT GACCTGGGAG
    GACTTTGAAC TGGTAAACCC TCACAGAGCA CTGTTTCTTA AAGAGCTGAA GGCACTTTCT
    GTGAAGCGCA GGCAGATTCT AGGCAATAAG AGTCTGTCCG AGGATGAGAA GAACACACGG
    CTTCAGGATC TCATGCTGAA GAATCCTATG GGCTCTGGCC CTCCACTGTG TGTTGAAGAT
    CTAGGATTAA ATTTCCAGTT CTGTCCATCA TCCAAAGTAC ATGGTTTCTC TTCTGTAGAC
    TTGAAGCCTA ATGGAGAGGA TGAGATGGTC ACCATGGACA ATGCAGAGGA GTATGTAGAG
    CTGATGTTTG ACTTCTGCAT GCACACAGGA ATCCAGAAGC AAATGGAGGC CTTTAGAGAG
    GGCTTTAATA AAGTGTTCCC TATGGAGAAA CTGAGCTCAT TTAGCCATAA AGAGGTGCAG
    ATGATCCTGT GTGGAAACCA GTCTCCATCC TGGACTGCTG AAGACATTGT TAACTACACA
    GAACCTAAAC TGGGCTATAC ACGGGACAGT CCTGGTTTCC TGCGTTTCGT GCGGGTTCTG
    TGTGGAATGT CTTCTGATGA GAGGAAAGCT TTCCTCCAGT TCACTACAGG CTGCTCAACT
    CTGCCCCCTG GTGGACTGGC CAACCTCCAT CCACGGCTCA CAATAGTCCG AAAGGTTGAT
    GCAACAGATG CCAGCTATCC TTCAGTCAAC ACGTGTGTGC ACTACTTGAA GCTGCCGGAA
    TATTCTTCAG AAGAGATTAT GAGAGAGCGC CTTCTCGCTG CCACTATGGA GAAGGGTTTC
    CACCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291392.1
    CDS463..8034
    Translation

    Target ORF information:

    RefSeq Version XM_009293117.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293117.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGAGGAA
    GAGGAGTATG AAACCAAAGG GGGCCGTCGC AGGACCTGGG ATGACGACTT TGTGCTGAAA
    CGACAGTTTT CTGCTTTAGT ACCTGCATTT GACCCTAGAC CAGGCAGGAC CAACGTCCAG
    CAAACTACTG ACCTGGAAAT CCCCCCACCA GGAACACCTC GTTCAGAGGT ACAGGAGGAG
    GTGGAGTGCG CTCCTTCACC TCGTCTGGCT CTCATCTTAA AAGTAGCAGG TCTAGGAACT
    ACTCGAGAAG TAGAACTACC TCTCACCAAT TACAAGTCCA CCATTTTCTA TTATGTCCAA
    AAACTTCTCC AGCTGTCATG CAACGGTGCT ATTAAACCTG ACAAGCTCAG ACGCATCTGG
    GAGCCTACGT ATACGATAAT GTATAGAGAA TTGAAGGATT CCGACAAAGA AAGAGAGAGC
    GGAAAGATGG GTTGCTGGTC TGTAGAGCAT GTGGAACAGT ACCTTGGCAC AGATGAATTA
    CCAAAGAATG ACTTGATAAC CTACATGCAG AAGAATGCAG ACTCAACTTT CCTGCGCCAC
    TGGAAATTAA CCGGCTCTAA TAAAAGTATT AGGAAAAACA GAAATTGTTC GCAGCTCATA
    GCTGCATACA AGGACTTCTG TGAGCGTGGC TGCAGATCTT CAGGCCTGAG TTCAGGGACG
    TTGTCCACGA CGCAAAGCTG TGATATCTTG AGTGCTGCAA GAGAGCAGGC TCAGGCTAAA
    GCGGGCTCGG GTCAGAGCGC CTGCAGCGTA GAGGATGTAC TGCAGCTCTT GCGCATCCTT
    TTCACCATCG GAGGAGAACC TACATCTGGA CGCACACTAC AGGAAGATGT GGAAGAGCTA
    CAGTTCAATG CATCACCTGA GGAATTCACC AGCAAAAAAA TTACAACCAA AATCCTGCAG
    CAGATTGAAG AGCCATTGGC TCTGGCTAGT GGGGCTCTCC CCGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT CATACCCTTT GAAACCCGGC AGCTTTATTT TACCTGCACT
    GCATTTGGAG CCTCTAGGGC AATTGTTTGG CTCCAGAATC GAAGGGAAGC CACTATGGAG
    CGTTCCCGGC CATCCACGAC TGTTCGCCGT GATGATCCCG GAGAGTTCCG TGTTGGTCGG
    CTTAAGCATG AACGTGTCAA GGTGCCTCGA GGAGAGAGTA TGATGGAGTG GGCTGAGAGT
    GTGATGCAGA TACACGCTGA CAGAAAGTCT GTACTGGAGG TGGAGTTCCA AGGGGAGGAG
    GGCACTGGTC TCGGACCCAC CCTGGAGTTC TATGCTCTTG TTGCGGCAGA GTTTCAGAGG
    ACCTCCCTTG GCATCTGGCT CTGTGATGAT GACTTCCCAG ATGATGAGTC ACGTCAGGTG
    GATCTGGGCG GTGGCTTGAA ACCGCCAGGC TATTACGTGC AGCGTTCCTG CGGCCTTTTC
    CCTGCTCCCT TCCCTCAGGA CAGCGATGAA CTGGAGCGTA TTACCAAACT TTTCTTGTTC
    CTTGGCATCT TTCTGGCCAA ATGCATCCAG GACAACCGGC TCGTGGATCT GCCCATATCC
    CAGCCTTTCT TTAAACTGCT CTGTATGGGT GACATCAAAA GCAACATGAG TAAGCTACTT
    TATCAAACCC GTGGTGAGTC GGACTGTCAC TTCTCTGAAA TCCAGTCTGA GGCCTCCACC
    GAGGAGGGTC AGGACACGTA CTCTGTGGGT AGTTTTGATG AAGACTCCAA GTCTGAGTTT
    ATTCTTGACC CACCGAAGCC CAAACCACCA GCCTGGTATC ATGGCATCTT GACCTGGGAG
    GACTTTGAAC TGGTAAACCC TCACAGAGCA CTGTTTCTTA AAGAGCTGAA GGCACTTTCT
    GTGAAGCGCA GGCAGATTCT AGGCAATAAG AGTCTGTCCG AGGATGAGAA GAACACACGG
    CTTCAGGATC TCATGCTGAA GAATCCTATG GGCTCTGGCC CTCCACTGTG TGTTGAAGAT
    CTAGGATTAA ATTTCCAGTT CTGTCCATCA TCCAAAGTAC ATGGTTTCTC TTCTGTAGAC
    TTGAAGCCTA ATGGAGAGGA TGAGATGGTC ACCATGGACA ATGCAGAGGA GTATGTAGAG
    CTGATGTTTG ACTTCTGCAT GCACACAGGA ATCCAGAAGC AAATGGAGGC CTTTAGAGAG
    GGCTTTAATA AAGTGTTCCC TATGGAGAAA CTGAGCTCAT TTAGCCATAA AGAGGTGCAG
    ATGATCCTGT GTGGAAACCA GTCTCCATCC TGGACTGCTG AAGACATTGT TAACTACACA
    GAACCTAAAC TGGGCTATAC ACGGGACAGT CCTGGTTTCC TGCGTTTCGT GCGGGTTCTG
    TGTGGAATGT CTTCTGATGA GAGGAAAGCT TTCCTCCAGT TCACTACAGG CTGCTCAACT
    CTGCCCCCTG GTGGACTGGC CAACCTCCAT CCACGGCTCA CAATAGTCCG AAAGGTTGAT
    GCAACAGATG CCAGCTATCC TTCAGTCAAC ACGTGTGTGC ACTACTTGAA GCTGCCGGAA
    TATTCTTCAG AAGAGATTAT GAGAGAGCGC CTTCTCGCTG CCACTATGGA GAAGGGTTTC
    CACCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47648
    Clone ID Related Accession (Same CDS sequence) XM_009293117.2 , XM_009293117.3
    Accession Version XM_009293117.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGAGGAA
    GAGGAGTATG AAACCAAAGG GGGCCGTCGC AGGACCTGGG ATGACGACTT TGTGCTGAAA
    CGACAGTTTT CTGCTTTAGT ACCTGCATTT GACCCTAGAC CAGGCAGGAC CAACGTCCAG
    CAAACTACTG ACCTGGAAAT CCCCCCACCA GGAACACCTC GTTCAGAGGT ACAGGAGGAG
    GTGGAGTGCG CTCCTTCACC TCGTCTGGCT CTCATCTTAA AAGTAGCAGG TCTAGGAACT
    ACTCGAGAAG TAGAACTACC TCTCACCAAT TACAAGTCCA CCATTTTCTA TTATGTCCAA
    AAACTTCTCC AGCTGTCATG CAACGGTGCT ATTAAACCTG ACAAGCTCAG ACGCATCTGG
    GAGCCTACGT ATACGATAAT GTATAGAGAA TTGAAGGATT CCGACAAAGA AAGAGAGAGC
    GGAAAGATGG GTTGCTGGTC TGTAGAGCAT GTGGAACAGT ACCTTGGCAC AGATGAATTA
    CCAAAGAATG ACTTGATAAC CTACATGCAG AAGAATGCAG ACTCAACTTT CCTGCGCCAC
    TGGAAATTAA CCGGCTCTAA TAAAAGTATT AGGAAAAACA GAAATTGTTC GCAGCTCATA
    GCTGCATACA AGGACTTCTG TGAGCGTGGC TGCAGATCTT CAGGCCTGAG TTCAGGGACG
    TTGTCCACGA CGCAAAGCTG TGATATCTTG AGTGCTGCAA GAGAGCAGGC TCAGGCTAAA
    GCGGGCTCGG GTCAGAGCGC CTGCAGCGTA GAGGATGTAC TGCAGCTCTT GCGCATCCTT
    TTCACCATCG GAGGAGAACC TACATCTGGA CGCACACTAC AGGAAGATGT GGAAGAGCTA
    CAGTTCAATG CATCACCTGA GGAATTCACC AGCAAAAAAA TTACAACCAA AATCCTGCAG
    CAGATTGAAG AGCCATTGGC TCTGGCTAGT GGGGCTCTCC CCGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT CATACCCTTT GAAACCCGGC AGCTTTATTT TACCTGCACT
    GCATTTGGAG CCTCTAGGGC AATTGTTTGG CTCCAGAATC GAAGGGAAGC CACTATGGAG
    CGTTCCCGGC CATCCACGAC TGTTCGCCGT GATGATCCCG GAGAGTTCCG TGTTGGTCGG
    CTTAAGCATG AACGTGTCAA GGTGCCTCGA GGAGAGAGTA TGATGGAGTG GGCTGAGAGT
    GTGATGCAGA TACACGCTGA CAGAAAGTCT GTACTGGAGG TGGAGTTCCA AGGGGAGGAG
    GGCACTGGTC TCGGACCCAC CCTGGAGTTC TATGCTCTTG TTGCGGCAGA GTTTCAGAGG
    ACCTCCCTTG GCATCTGGCT CTGTGATGAT GACTTCCCAG ATGATGAGTC ACGTCAGGTG
    GATCTGGGCG GTGGCTTGAA ACCGCCAGGC TATTACGTGC AGCGTTCCTG CGGCCTTTTC
    CCTGCTCCCT TCCCTCAGGA CAGCGATGAA CTGGAGCGTA TTACCAAACT TTTCTTGTTC
    CTTGGCATCT TTCTGGCCAA ATGCATCCAG GACAACCGGC TCGTGGATCT GCCCATATCC
    CAGCCTTTCT TTAAACTGCT CTGTATGGGT GACATCAAAA GCAACATGAG TAAGCTACTT
    TATCAAACCC GTGGTGAGTC GGACTGTCAC TTCTCTGAAA TCCAGTCTGA GGCCTCCACC
    GAGGAGGGTC AGGACACGTA CTCTGTGGGT AGTTTTGATG AAGACTCCAA GTCTGAGTTT
    ATTCTTGACC CACCGAAGCC CAAACCACCA GCCTGGTATC ATGGCATCTT GACCTGGGAG
    GACTTTGAAC TGGTAAACCC TCACAGAGCA CTGTTTCTTA AAGAGCTGAA GGCACTTTCT
    GTGAAGCGCA GGCAGATTCT AGGCAATAAG AGTCTGTCCG AGGATGAGAA GAACACACGG
    CTTCAGGATC TCATGCTGAA GAATCCTATG GGCTCTGGCC CTCCACTGTG TGTTGAAGAT
    CTAGGATTAA ATTTCCAGTT CTGTCCATCA TCCAAAGTAC ATGGTTTCTC TTCTGTAGAC
    TTGAAGCCTA ATGGAGAGGA TGAGATGGTC ACCATGGACA ATGCAGAGGA GTATGTAGAG
    CTGATGTTTG ACTTCTGCAT GCACACAGGA ATCCAGAAGC AAATGGAGGC CTTTAGAGAG
    GGCTTTAATA AAGTGTTCCC TATGGAGAAA CTGAGCTCAT TTAGCCATAA AGAGGTGCAG
    ATGATCCTGT GTGGAAACCA GTCTCCATCC TGGACTGCTG AAGACATTGT TAACTACACA
    GAACCTAAAC TGGGCTATAC ACGGGACAGT CCTGGTTTCC TGCGTTTCGT GCGGGTTCTG
    TGTGGAATGT CTTCTGATGA GAGGAAAGCT TTCCTCCAGT TCACTACAGG CTGCTCAACT
    CTGCCCCCTG GTGGACTGGC CAACCTCCAT CCACGGCTCA CAATAGTCCG AAAGGTTGAT
    GCAACAGATG CCAGCTATCC TTCAGTCAAC ACGTGTGTGC ACTACTTGAA GCTGCCGGAA
    TATTCTTCAG AAGAGATTAT GAGAGAGCGC CTTCTCGCTG CCACTATGGA GAAGGGTTTC
    CACCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291392.1
    CDS463..8034
    Translation

    Target ORF information:

    RefSeq Version XM_009293117.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain containing 1 (hectd1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293117.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCGGACG TGGACCCGGA CACGTTGCTG GAATGGTTGC AGATGGGACA GGGCGATGAG 
    CGGGATATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGTTGCTCAT GTCAGACAAT
    GTGGACCGCT GCTTCGAAAC GTGTCCTCCA CGGACGTTTC TTCCAGCACT GTGTAAGATC
    TTTCTGGATG AGAGTGCACC CGATAATGTG CTGGAAGTGA CAGCCAGAGC CATCACCTAC
    TACCTGGACG TGTCCGCTGA ATGCACTCGC AGGATTGTGG GAGTAGATGG AGCCATCAAA
    GCCCTGTGTA ACCGGCTGGT GGTGGTGGAG CTCAACAACA GGACCAGCAG AGATCTGGCT
    GAACAATGTG TAAAGGTTCT GGAGCTGATC TGCACTCGGG AGTCTGGAGC TGTATTTGAA
    GCAGGAGGAC TGAACTGTGT GTTGAGCTTC ATTAGGGACA GCGGTCATCT TGTGCACAAA
    GACACATTGC ACTCTGCCAT GGCAGTAGTT TCTCGCCTCT GCAGTAAGAT GGAGCCACAG
    GACTCTTCCC TTGAGACATG CGTGGAGTCC CTCTCTAGTC TGCTGAAACA TGAAGACCAT
    CAGGTTTCTG ATGGAGCTCT GCGCTGCTTC GCTTCACTGG CGGACCGGTT TACAAGACGT
    GGAGTTGACC CAGCCCCTTT AGCAAAGCAT GGACTGACAG AAGAGTTGCT GTCCCGTATG
    GCTGCGGCAG GAGGTACTGC ATCTGGACCC TCCTCCACCT GCAAGCCTGG CAGAACCTCT
    AGTGGAGCCG CTCCATCTGC TGCAGACTCG AAACTGAGCA ACCAAGTGTC CACCATCGTC
    AGCCTGCTGT CCACACTCTG CAGAGGCTCA CCACTTGTCA CGCATGATCT TCTGCGCTCA
    GAGCTGCCCG ACTCTATGGA GAGTGCTCTA CAGGGGGACG AGCGGTGTGT ACTGGACACT
    ATGCGGCTGG TGGACTTACT GCTGGTGTTG CTCTTTGAAG GCCGCAAGGC TTTGCCCAAA
    TCTACAGCTG GGTCTACAGG CCGTATTCCT GGGCTGCGGC GTCTCGACAG CTCCGGGGAA
    CGCTCCCACC GACAGCTTAT TGACTGTATA CGCAGCAAAG ACACCGATGC TCTCATTGAT
    GCCATTGATA CCGGTGCATT TGAAGTGAAT TTCATGGATG ATGTAGGACA GACTCTCTTA
    AACTGGGCGT CTGCATTTGG AACGCAAGAA ATGGTGGAGT TCCTTTGCGA GAGAGGAGCT
    GATGTCAATA GAGGTCAAAG GTCATCCTCC CTGCACTATG CTGCCTGTTT TGGCAGGCCA
    CAAGTAGCCA AGACTCTATT GCGCCATGGG GCCAACCCTG ACCTGAGAGA TGAAGATGGG
    AAAACTCCTT TAGACAAAGC TAGAGAGAGG GGACACAGCG AGGTTGTAGC AATTCTCCAG
    TCTCCTGGAG ACTGGATGTG CCCTGTAAAC AAAGGGGATG ACAAGAAAAA GAAGGATGTC
    AATAAAGAAG AGGAGGAAGG CAGTGAGCCG AAAGGTGACC CTGAAATGGC GCCAATCTAC
    CTGAAAAGGC TACTTCCAGT ATTTGCACAG ACCTTTCAGC AAACCATGCT GCCTTCAATA
    AGGAAAGCTA GTTTAGCTCT GATCAGAAAA ATGGTGCACT ACAGTTCTGA AGTTCTGCTT
    AAGGAAGTGT GTGATTCTGA TGCTGGACAC AACTTGCCCA CTGTACTTGT GGAGATCACT
    GCGACTGTGC TGGATCAGGA GGATGATGAT GATGGTCACC TGCTGGCACT TCAGATCATT
    AGGGATCTAG TGGATAAGGG AGGTGACGTG TTCTTAGATC AGCTGGCCAG GCTGGGTGTC
    ATTAATAAGG TGTCCACTCT GGCAGGACCC ACGTCAGATG ACGAAAATGA GGAAGAGGCC
    AAGCCTGAGA AGGATGACGA ACCACAAGAG GATGCCAAAG AGATTCAACA GGGAAAGCCT
    TACCACTGGA GAGACTGGTC CATCATCAGA GGCAGAGACT GCCTTTATAT CTGGAGTGAC
    GCAGCGGCTT TGGAGCTCTC CAATGGCAGC AATGGTTGGT TCCGTTTTAT CCTAGATGGT
    AAACTAGCAA CCATGTACTC CAGTGGAAGC CCAGAGGGAG GCTCTGACAG CTCAGAAAGC
    AGAAGTGAGT TTCTTGAGAA GCTGCAGCGT GCCAGAAGTC AAGTCAAACC AGTGACAGCT
    AGTCAGCCCA TCCTGTCCAC ACAAGGGCCC ACCAAACTCA CTGTGGGTAA TTGGTCTCTC
    ACTTGTCTGA AAGAAGGTGA GATTGCCATC CATAACTCAG ATGGCCAGCA GGCTACCATC
    CTCAAAGAGG ACTTGCCAGG CTTTGTGTTC GAGTCAAATC GAGGCACTAA GCACTCTTTC
    ACTGCAGAAA CTTCTCTAGG GTCTGAATTT GTTACTGGCT GGACTGGTAA GCGAGGAAGA
    AAGCTGAAAT CCAAATTGGA AAAAACAAAA CAGAAGGTGA AAACCATGGC CAGAGATCTG
    TATGATGATC ATTTCAAGGC TGTGGAGAGC ATGCCGAGAG GTGTAGTTGT CACTTTGAGA
    AACATTGCCA CTCAGCTGGA GTCTGCTTGG GAGCTACATA CTAACAGACA GTGCATTGAA
    GGTGAAAACA CATGGCGAGA CCTCATGAAA ACAGCACTAG AGAATTTAAT AGTAGTTCTC
    AAGGATGAGA ACACCATTTC TCCTTATGAA ATGTGCAGCA GTGGCCTCGT ACAAGCGCTT
    TTTACTGTCC TCAGCAATAG TGTGGAACTT GACATAAAAC ATGATTGTAA GCCTCTGATG
    GAAAGAATAA ATGTGTTCAA GACTGCATTC ACTGAAAATG AAGATGACGA AAGCCGACCC
    GCAGTTGCCT TAATCCGCAA ACTGATAGCA GTGCTGGAAT CAATAGAGCG GCTACCTTTG
    CACTTATATG ATACCCCAGG CTCAACATAT AATTTACAGA TACTGACAAG GAGGTTACGC
    TTCCGCCTGG AACGTGCTCC AGGTGAAACA GCCCTGATTG ATCGAACTGG AAGAATGTTG
    AAGATGGAGC CGCTGGCCAC TGTTGAGTCA CTTGAGCAGT ATCTGCTCAA GATGGTGGCC
    AAGCAGTGGT ATGACTTTGA CCGGTCCTCA TTCATCTTCG TTAGGAAGCT CAGAGAGGGC
    CAGAACTTCA CCTTCAGGCA CCAGCATGAC TTTGATGAGA ATGGAATTGT GTATTGGATT
    GGTACCAATG CAAAGACTGC GTATGAATGG GTGAACCCTG CTGCTTATGG ATTAGTGGTT
    GTGACCTCCT CCGAGGGCAG AAACTTGCCT TATGGCCGAC TTGAAGACAT CTTGAGCAGA
    GACAGCTCCG CCCTCAACTG TCACACCAAC GATGATAAAA ACGCCTGGTT TGCCATTGAC
    CTCGGTTTAT GGTTTGTCCC CTCTGCATAC ACACTTCGTC ACGCTCGTGG ATACGGTCGG
    TCCGCTCTCA GAAATTGGGT GTTTCAAGTG TCCAAAGATG GTCAGAACTG GATGACTCTC
    TACACCCACG TAGATGACAG CTCTCTCAAT GAACCAGGGT CAACAGCCAC ATGGCCTCTG
    GATCCATCCA AAGATGAGAA GCAGGGCTGG AGACACATTC GTATTAAACA GATGGGAAAG
    AATGCCAGCG GACAAACACA CTACCTCTCC CTGTCTGGTC TAGAGATCTA TGGCACTGTT
    ACTGGTGTGT GTGAGGATCA GCTGGGTAAA GCAGTGAAAG AGGCAGAGGC CAACCTGAGA
    CGGCAGCGGC GACTCTTTCG CTCGCAGGTG ATGAAGTACA TTGTTCCAGG AGCGCGGGTG
    GTGCGAGGCA TTGACTGGAA GTGGCGAGAC CAGGATGGCA ATCCTGCTGG AGAAGGCACT
    GTGACTGGAG AAGCCCACAA TGGCACACCG CAGTCCTGGA GCAGCCTGGT GAAAAATAAC
    TGTCCAGACA AGGGTGGCTC ATCCTCCACA GCGGGGGCCA GTTCCTCCAG CCGCAAGGGT
    AGTAGCAGTT CCGTGTGTAG CGTGGCCAGC AGCAGCGATA TAAGCCTCAG CTCCACCCGA
    GTGGAGCGCA GGGTCGAGAG CCTGTTCGAG CAGGGAGTAG GCGTCATCGG AGGGCCCCCA
    GGAGCTGAGG GCCAAGAACC CATAGTTGTG CTTTCCTCAG CCGAAGCTGG CTCCGCCTCC
    AGCACCAGCA CATTGACAGC AGACACAGGA AGCGAAAGCG AGCGTAAAAC ACCAGGACCT
    GATGGCACTA GACAGTCAGC GGAGTCCACA GCCATCTCTA TGGGAATCGT GAGCGTGAGC
    TCGCCTGATG TCAGCTCTGT TTCAGAGTCG TCTAGTAAAG ACGCTGCATC CCAGAGGCCC
    TTGTGCTCTG CCACCAGTGC GCGGCTGTCT GTCAGTTCAC TTTTGGCAGC TGGAGCACCC
    ATGAGTTCTA GTGCCAGCGT CCCCAACCTG TCCTCTCGCG AGGCCAGCCT GATGGAGTCG
    TTCGTTCGGA GGGCACCCAA CATGTCTCGC ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGAAGCAGCA GTGATAACAA CACTAACACG CTGGGACGAA ATGTCATGAG CACTGCCACT
    TCTCCACTTA TGGGTGCGCA GAGCTTTCCT AACCTTACAA CCACTGGTAC CACCTCAACT
    GTTACAATGT CCACCTCTAT AGTAACCAGC AGCAATAATG TAGCCACGGC AACTACAGGT
    TTGTCCGTCG GCCAGTTGCT CAGTAACACG CTGACGACCA GCCTGACCTC TACATCTAGC
    GAGAGTGACA CAGGTCAGGA GGCTGAATTT TCCCTCTATG ACTTCCTGGA CAGTTGTCGG
    GCCAACACTT TGCTTGCAGA GTTGGATGAT GAAGAAGATT TGCCGGAGCC AGATGATGAC
    GATGATGAAA ATGAGGATGA CAATCAGGAG GAACAGGAGT ATGAGGAAGT TTTGGAGGAA
    GAGGAGTATG AAACCAAAGG GGGCCGTCGC AGGACCTGGG ATGACGACTT TGTGCTGAAA
    CGACAGTTTT CTGCTTTAGT ACCTGCATTT GACCCTAGAC CAGGCAGGAC CAACGTCCAG
    CAAACTACTG ACCTGGAAAT CCCCCCACCA GGAACACCTC GTTCAGAGGT ACAGGAGGAG
    GTGGAGTGCG CTCCTTCACC TCGTCTGGCT CTCATCTTAA AAGTAGCAGG TCTAGGAACT
    ACTCGAGAAG TAGAACTACC TCTCACCAAT TACAAGTCCA CCATTTTCTA TTATGTCCAA
    AAACTTCTCC AGCTGTCATG CAACGGTGCT ATTAAACCTG ACAAGCTCAG ACGCATCTGG
    GAGCCTACGT ATACGATAAT GTATAGAGAA TTGAAGGATT CCGACAAAGA AAGAGAGAGC
    GGAAAGATGG GTTGCTGGTC TGTAGAGCAT GTGGAACAGT ACCTTGGCAC AGATGAATTA
    CCAAAGAATG ACTTGATAAC CTACATGCAG AAGAATGCAG ACTCAACTTT CCTGCGCCAC
    TGGAAATTAA CCGGCTCTAA TAAAAGTATT AGGAAAAACA GAAATTGTTC GCAGCTCATA
    GCTGCATACA AGGACTTCTG TGAGCGTGGC TGCAGATCTT CAGGCCTGAG TTCAGGGACG
    TTGTCCACGA CGCAAAGCTG TGATATCTTG AGTGCTGCAA GAGAGCAGGC TCAGGCTAAA
    GCGGGCTCGG GTCAGAGCGC CTGCAGCGTA GAGGATGTAC TGCAGCTCTT GCGCATCCTT
    TTCACCATCG GAGGAGAACC TACATCTGGA CGCACACTAC AGGAAGATGT GGAAGAGCTA
    CAGTTCAATG CATCACCTGA GGAATTCACC AGCAAAAAAA TTACAACCAA AATCCTGCAG
    CAGATTGAAG AGCCATTGGC TCTGGCTAGT GGGGCTCTCC CCGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT CATACCCTTT GAAACCCGGC AGCTTTATTT TACCTGCACT
    GCATTTGGAG CCTCTAGGGC AATTGTTTGG CTCCAGAATC GAAGGGAAGC CACTATGGAG
    CGTTCCCGGC CATCCACGAC TGTTCGCCGT GATGATCCCG GAGAGTTCCG TGTTGGTCGG
    CTTAAGCATG AACGTGTCAA GGTGCCTCGA GGAGAGAGTA TGATGGAGTG GGCTGAGAGT
    GTGATGCAGA TACACGCTGA CAGAAAGTCT GTACTGGAGG TGGAGTTCCA AGGGGAGGAG
    GGCACTGGTC TCGGACCCAC CCTGGAGTTC TATGCTCTTG TTGCGGCAGA GTTTCAGAGG
    ACCTCCCTTG GCATCTGGCT CTGTGATGAT GACTTCCCAG ATGATGAGTC ACGTCAGGTG
    GATCTGGGCG GTGGCTTGAA ACCGCCAGGC TATTACGTGC AGCGTTCCTG CGGCCTTTTC
    CCTGCTCCCT TCCCTCAGGA CAGCGATGAA CTGGAGCGTA TTACCAAACT TTTCTTGTTC
    CTTGGCATCT TTCTGGCCAA ATGCATCCAG GACAACCGGC TCGTGGATCT GCCCATATCC
    CAGCCTTTCT TTAAACTGCT CTGTATGGGT GACATCAAAA GCAACATGAG TAAGCTACTT
    TATCAAACCC GTGGTGAGTC GGACTGTCAC TTCTCTGAAA TCCAGTCTGA GGCCTCCACC
    GAGGAGGGTC AGGACACGTA CTCTGTGGGT AGTTTTGATG AAGACTCCAA GTCTGAGTTT
    ATTCTTGACC CACCGAAGCC CAAACCACCA GCCTGGTATC ATGGCATCTT GACCTGGGAG
    GACTTTGAAC TGGTAAACCC TCACAGAGCA CTGTTTCTTA AAGAGCTGAA GGCACTTTCT
    GTGAAGCGCA GGCAGATTCT AGGCAATAAG AGTCTGTCCG AGGATGAGAA GAACACACGG
    CTTCAGGATC TCATGCTGAA GAATCCTATG GGCTCTGGCC CTCCACTGTG TGTTGAAGAT
    CTAGGATTAA ATTTCCAGTT CTGTCCATCA TCCAAAGTAC ATGGTTTCTC TTCTGTAGAC
    TTGAAGCCTA ATGGAGAGGA TGAGATGGTC ACCATGGACA ATGCAGAGGA GTATGTAGAG
    CTGATGTTTG ACTTCTGCAT GCACACAGGA ATCCAGAAGC AAATGGAGGC CTTTAGAGAG
    GGCTTTAATA AAGTGTTCCC TATGGAGAAA CTGAGCTCAT TTAGCCATAA AGAGGTGCAG
    ATGATCCTGT GTGGAAACCA GTCTCCATCC TGGACTGCTG AAGACATTGT TAACTACACA
    GAACCTAAAC TGGGCTATAC ACGGGACAGT CCTGGTTTCC TGCGTTTCGT GCGGGTTCTG
    TGTGGAATGT CTTCTGATGA GAGGAAAGCT TTCCTCCAGT TCACTACAGG CTGCTCAACT
    CTGCCCCCTG GTGGACTGGC CAACCTCCAT CCACGGCTCA CAATAGTCCG AAAGGTTGAT
    GCAACAGATG CCAGCTATCC TTCAGTCAAC ACGTGTGTGC ACTACTTGAA GCTGCCGGAA
    TATTCTTCAG AAGAGATTAT GAGAGAGCGC CTTCTCGCTG CCACTATGGA GAAGGGTTTC
    CACCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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