pik3ap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pik3ap1
Entrez Gene ID	100002256
Full Name	phosphoinositide-3-kinase adaptor protein 1
Synonyms	si:dkey-226b20.7
General protein information	Preferred Names phosphoinositide-3-kinase adaptor protein 1 Names phosphoinositide 3-kinase adapter protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358966.2	XP_017214455.1	phosphoinositide 3-kinase adapter protein 1
XM_009307382.3	XP_009305657.1	phosphoinositide 3-kinase adapter protein 1
XM_009307382.2	XP_009305657.1	phosphoinositide 3-kinase adapter protein 1
XM_017358966.1	XP_017214455.1	phosphoinositide 3-kinase adapter protein 1
XM_021474293.1	XP_021329968.1	phosphoinositide 3-kinase adapter protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PIK3AP1	XP_850837.1
Rattus norvegicus (Norway rat)	Pik3ap1	NP_001099838.1
Homo sapiens (human)	PIK3AP1	NP_689522.2
Bos taurus (cattle)	PIK3AP1	XP_002698457.2
Danio rerio (zebrafish)	pik3ap1	XP_005156973.1
Xenopus tropicalis (tropical clawed frog)	pik3ap1	XP_002936021.2
Pan troglodytes (chimpanzee)	PIK3AP1	XP_507955.2
	PIK3AP1	XP_001101530.1
Mus musculus (house mouse)	Pik3ap1	NP_113553.1
Gallus gallus (chicken)	PIK3AP1	NP_989681.1

Gene Ontology

The following pik3ap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pik3ap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18852	XM_009307382.3 Latest version!	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa18852	XM_017358966.2 Latest version!	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa146684	XM_021474293.1 Latest version!	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa18852	XM_009307382.2	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa18852	XM_017358966.1	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pik3ap1 ( XM_017358966.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214455.1 Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18852
Clone ID Related Accession (Same CDS sequence)	XM_017358966.2 , XM_009307382.3 , XM_009307382.2 , XM_017358966.1
Accession Version	XM_017358966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214455.1
CDS	869..3382
Translation	MCEVLIVHSGEAREWADYLQQILAASCSFQEGSVVLYDVDEEICLKNYGPFGSSKCILLLLTSGLVNLQNDSVVLDTFRDLLHPPHKVVAFLCGVSEELMSGYFEYWEQWRKIFSEDEPQVYVSTIRESIDEGSVLQHQCQNTYEIEPEEPLEDLPLPDFSEILPLPDELNEHQLNGDEVCSFLQTSLNTSNDQICLSVQPDRILCGSGVSIFVMLVQKCEDEDSVEVEFHSQQSPPLRLPGTLVNQCIVTVQSPDMPAGEVSLTVYKNQSVLCSSTVTYYTEMEEICRYLKKATDPVQFMCQAFDITSKLQESLDNLLATSLEERMPVNGLQVFGISQIEENTAANHRDVELPTLLHFSAKYGLKKLTSLLMRCPGAMQAYSVMNKDGDYPNKLAEKSGFSDLRQFMDEYVETVDLVKTHIEESQNAPESEDIYEDMLRASQNFISEFDNENIYESMVKLNPEMGDELESALGDSNPERMLRKFFEGPPEKGFQSMKHNSTNGGLPEMDDEDYHDMGVKVDVYGDFEEEDPYNPCCPEQIYDTVEPHSSPEVMNRPPAPIPRPAKLPEPEENTTYISKVFCPKDPLYSVSQRPERDPSIAPVRLVRDRDMSSHHDPFAGMKTPGQRQLISLQERVKVGELTVEEAVQEFKAWQFDQDKRWRSLRFQQENLHRLRESITRRCKDKGTNELMITAPMQTNTQWGSQMNVNCSVYEPTPRTVVQPPSVNRAPQRGSWQTGSTSSTSSSSSHRLSIQSTVSNSSGVDGEEEPPDIPLPPRPLRPVVDSPPILPPPRVPPRVIERSPEILNERYVSNPSRPVPQNPAQRPIPPPPVPRRTW

Target ORF information:

RefSeq Version	XM_017358966.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358966.2

ORF Insert Sequence:

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3ap1 ( XM_009307382.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305657.1 Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18852
Clone ID Related Accession (Same CDS sequence)	XM_017358966.2 , XM_009307382.3 , XM_009307382.2 , XM_017358966.1
Accession Version	XM_009307382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305657.1
CDS	800..3313
Translation	MCEVLIVHSGEAREWADYLQQILAASCSFQEGSVVLYDVDEEICLKNYGPFGSSKCILLLLTSGLVNLQNDSVVLDTFRDLLHPPHKVVAFLCGVSEELMSGYFEYWEQWRKIFSEDEPQVYVSTIRESIDEGSVLQHQCQNTYEIEPEEPLEDLPLPDFSEILPLPDELNEHQLNGDEVCSFLQTSLNTSNDQICLSVQPDRILCGSGVSIFVMLVQKCEDEDSVEVEFHSQQSPPLRLPGTLVNQCIVTVQSPDMPAGEVSLTVYKNQSVLCSSTVTYYTEMEEICRYLKKATDPVQFMCQAFDITSKLQESLDNLLATSLEERMPVNGLQVFGISQIEENTAANHRDVELPTLLHFSAKYGLKKLTSLLMRCPGAMQAYSVMNKDGDYPNKLAEKSGFSDLRQFMDEYVETVDLVKTHIEESQNAPESEDIYEDMLRASQNFISEFDNENIYESMVKLNPEMGDELESALGDSNPERMLRKFFEGPPEKGFQSMKHNSTNGGLPEMDDEDYHDMGVKVDVYGDFEEEDPYNPCCPEQIYDTVEPHSSPEVMNRPPAPIPRPAKLPEPEENTTYISKVFCPKDPLYSVSQRPERDPSIAPVRLVRDRDMSSHHDPFAGMKTPGQRQLISLQERVKVGELTVEEAVQEFKAWQFDQDKRWRSLRFQQENLHRLRESITRRCKDKGTNELMITAPMQTNTQWGSQMNVNCSVYEPTPRTVVQPPSVNRAPQRGSWQTGSTSSTSSSSSHRLSIQSTVSNSSGVDGEEEPPDIPLPPRPLRPVVDSPPILPPPRVPPRVIERSPEILNERYVSNPSRPVPQNPAQRPIPPPPVPRRTW

Target ORF information:

RefSeq Version	XM_009307382.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307382.3

ORF Insert Sequence:

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3ap1 ( XM_009307382.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305657.1 Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18852
Clone ID Related Accession (Same CDS sequence)	XM_017358966.2 , XM_009307382.3 , XM_009307382.2 , XM_017358966.1
Accession Version	XM_009307382.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305657.1
CDS	798..3311
Translation	MCEVLIVHSGEAREWADYLQQILAASCSFQEGSVVLYDVDEEICLKNYGPFGSSKCILLLLTSGLVNLQNDSVVLDTFRDLLHPPHKVVAFLCGVSEELMSGYFEYWEQWRKIFSEDEPQVYVSTIRESIDEGSVLQHQCQNTYEIEPEEPLEDLPLPDFSEILPLPDELNEHQLNGDEVCSFLQTSLNTSNDQICLSVQPDRILCGSGVSIFVMLVQKCEDEDSVEVEFHSQQSPPLRLPGTLVNQCIVTVQSPDMPAGEVSLTVYKNQSVLCSSTVTYYTEMEEICRYLKKATDPVQFMCQAFDITSKLQESLDNLLATSLEERMPVNGLQVFGISQIEENTAANHRDVELPTLLHFSAKYGLKKLTSLLMRCPGAMQAYSVMNKDGDYPNKLAEKSGFSDLRQFMDEYVETVDLVKTHIEESQNAPESEDIYEDMLRASQNFISEFDNENIYESMVKLNPEMGDELESALGDSNPERMLRKFFEGPPEKGFQSMKHNSTNGGLPEMDDEDYHDMGVKVDVYGDFEEEDPYNPCCPEQIYDTVEPHSSPEVMNRPPAPIPRPAKLPEPEENTTYISKVFCPKDPLYSVSQRPERDPSIAPVRLVRDRDMSSHHDPFAGMKTPGQRQLISLQERVKVGELTVEEAVQEFKAWQFDQDKRWRSLRFQQENLHRLRESITRRCKDKGTNELMITAPMQTNTQWGSQMNVNCSVYEPTPRTVVQPPSVNRAPQRGSWQTGSTSSTSSSSSHRLSIQSTVSNSSGVDGEEEPPDIPLPPRPLRPVVDSPPILPPPRVPPRVIERSPEILNERYVSNPSRPVPQNPAQRPIPPPPVPRRTW

Target ORF information:

RefSeq Version	XM_009307382.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307382.2

ORF Insert Sequence:

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3ap1 ( XM_017358966.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214455.1 Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18852
Clone ID Related Accession (Same CDS sequence)	XM_017358966.2 , XM_009307382.3 , XM_009307382.2 , XM_017358966.1
Accession Version	XM_017358966.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214455.1
CDS	202..2715
Translation	MCEVLIVHSGEAREWADYLQQILAASCSFQEGSVVLYDVDEEICLKNYGPFGSSKCILLLLTSGLVNLQNDSVVLDTFRDLLHPPHKVVAFLCGVSEELMSGYFEYWEQWRKIFSEDEPQVYVSTIRESIDEGSVLQHQCQNTYEIEPEEPLEDLPLPDFSEILPLPDELNEHQLNGDEVCSFLQTSLNTSNDQICLSVQPDRILCGSGVSIFVMLVQKCEDEDSVEVEFHSQQSPPLRLPGTLVNQCIVTVQSPDMPAGEVSLTVYKNQSVLCSSTVTYYTEMEEICRYLKKATDPVQFMCQAFDITSKLQESLDNLLATSLEERMPVNGLQVFGISQIEENTAANHRDVELPTLLHFSAKYGLKKLTSLLMRCPGAMQAYSVMNKDGDYPNKLAEKSGFSDLRQFMDEYVETVDLVKTHIEESQNAPESEDIYEDMLRASQNFISEFDNENIYESMVKLNPEMGDELESALGDSNPERMLRKFFEGPPEKGFQSMKHNSTNGGLPEMDDEDYHDMGVKVDVYGDFEEEDPYNPCCPEQIYDTVEPHSSPEVMNRPPAPIPRPAKLPEPEENTTYISKVFCPKDPLYSVSQRPERDPSIAPVRLVRDRDMSSHHDPFAGMKTPGQRQLISLQERVKVGELTVEEAVQEFKAWQFDQDKRWRSLRFQQENLHRLRESITRRCKDKGTNELMITAPMQTNTQWGSQMNVNCSVYEPTPRTVVQPPSVNRAPQRGSWQTGSTSSTSSSSSHRLSIQSTVSNSSGVDGEEEPPDIPLPPRPLRPVVDSPPILPPPRVPPRVIERSPEILNERYVSNPSRPVPQNPAQRPIPPPPVPRRTW

Target ORF information:

RefSeq Version	XM_017358966.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358966.1

ORF Insert Sequence:

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG CGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCAG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCCCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TACCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTGG GGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAACCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTTGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAT GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3ap1 ( XM_021474293.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329968.1 Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

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CloneID	ODa146684
Clone ID Related Accession (Same CDS sequence)	XM_021474293.1
Accession Version	XM_021474293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395309.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG AGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCGG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCTCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TATCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTAG AGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAGCCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTCGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAC GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329968.1
CDS	382..2895
Translation	MCEVLIVHSGEAREWADYLQQILEASCSFQEGSVVLYDVDEEICLKNYGPFGSSKCILLLLTSGLVNLQNDSVVLDTFRDLLHPPHKVVAFLCGVSEELMSGYFEYWEQWRKIFSEDEPQVYVSTIRESIDEGSVLQHQCQNTYEIEPEEPLEDLPLPDFSEILPLPDELNEHQLNGDEVCSFLQTGLNTSNDQICLSVQPDRILCGSGVSIFVMLVQKCEDEDSVEVEFHSQQSPPLRLPGTLVNQCIVTVQSPDMPAGEVSLTVYKNQSVLCSSTVTYYTEMEEICRYLKKATDPVQFMCQAFDITSKLQESLDNLLATSLEERMPVNGLQVFGISQIEENTAANHRDVELPTLLHFSAKYGLKKLTSLLMRCPGAMQAYSVMNKDGDYPNKLAEKSGFSDLRQFMDEYVETVDLVKTHIEESQNAPESEDIYEDMLRASQNFISEFDNENIYESMVKLNPEMGDELESALEDSNPERMLRKFFEGPPEKGFQSMKHNSTNGGLPEMDDEDYHDMGVKVDVYGDFEEEDPYNPCCPEQIYDTVEPHSSPEVMNRPPAPIPRPAKLPEPEENTTYISKVFCPKDPLYSVSQRPERDPSIAPVRLVRDRDMSSHHDPFAGMKTPGQRQLISLQERVKVGELTVEEAVQEFKAWQFDQDKRWRSLRFQQENLHRLRESITRRCKDKGTNELMITAPMQTNTQWGSQMNVNCSVYEPTPRTVVQPPSVNRAPQRGSWQTGSTSSTSSSSSHRLSIQSTVSNSSGVDGEEEPPDIPLPPRPLRPVVDSPPILPPPRVPPRVIERSPEILNERYVSNPSRPVPQNPAQRPIPPPPVPRRTW

Target ORF information:

RefSeq Version	XM_021474293.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase adaptor protein 1 (pik3ap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474293.1

ORF Insert Sequence:

ATGTGTGAGG TATTAATTGT TCACAGCGGC GAGGCCAGAG AATGGGCAGA TTATCTCCAG 
CAGATCCTGG AGGCCTCATG TAGCTTTCAG GAGGGCTCTG TCGTTCTGTA TGATGTGGAT 
GAAGAGATTT GTCTAAAAAA CTATGGACCT TTTGGCTCCA GCAAGTGCAT TCTTCTTCTG 
CTGACCTCTG GGCTCGTGAA CCTGCAGAAT GACTCTGTGG TGCTGGACAC TTTTCGGGAT 
CTTCTTCACC CTCCGCATAA AGTTGTGGCC TTTTTGTGTG GTGTTTCAGA AGAGCTAATG 
TCAGGGTATT TTGAGTACTG GGAGCAATGG AGAAAAATCT TTTCAGAAGA TGAGCCACAA 
GTTTATGTGT CCACCATCCG GGAGTCTATT GATGAGGGAT CTGTCCTGCA ACATCAGTGC 
CAGAATACAT ATGAAATTGA ACCAGAAGAG CCCTTGGAGG ACTTGCCGTT GCCCGACTTT 
TCTGAGATTT TACCTTTGCC AGATGAGCTG AATGAGCATC AGCTTAATGG GGATGAAGTG 
TGTTCATTTC TGCAAACCGG TTTAAATACC TCTAATGACC AAATCTGTCT CTCTGTCCAG 
CCTGACCGAA TACTGTGTGG GAGTGGTGTG AGTATTTTTG TAATGCTGGT GCAGAAGTGT 
GAGGATGAGG ACAGTGTGGA GGTGGAGTTT CACTCTCAGC AGTCGCCACC TCTGCGTCTG 
CCTGGAACTC TAGTCAACCA GTGCATTGTT ACTGTTCAGT CACCTGATAT GCCAGCTGGG 
GAAGTATCAT TGACGGTTTA TAAAAATCAG TCTGTGTTAT GCTCATCAAC TGTAACATAT 
TATACAGAAA TGGAGGAAAT TTGCAGATAT CTAAAGAAAG CAACTGATCC AGTGCAGTTC 
ATGTGTCAGG CATTTGATAT CACATCAAAG CTCCAAGAGT CGTTAGATAA TTTGCTTGCT 
ACCTCTCTTG AAGAACGGAT GCCTGTAAAT GGATTACAAG TATTCGGAAT TAGTCAAATT 
GAAGAAAACA CAGCAGCAAA TCACCGTGAT GTGGAACTCC CAACGCTGCT TCACTTTTCT 
GCCAAATATG GCTTAAAAAA GTTAACATCT TTGTTAATGC GGTGCCCCGG AGCCATGCAG 
GCTTACAGTG TGATGAACAA GGATGGGGAT TATCCCAATA AGCTGGCCGA GAAGAGCGGT 
TTCTCTGATT TAAGACAGTT CATGGATGAA TATGTTGAGA CAGTGGATTT AGTGAAGACG 
CACATTGAGG AATCTCAGAA CGCCCCCGAA AGTGAGGACA TCTATGAGGA TATGCTGAGA 
GCTTCCCAGA ACTTCATCTC TGAGTTCGAT AATGAGAACA TTTATGAGTC TATGGTGAAG 
CTCAATCCTG AGATGGGCGA TGAGTTAGAA AGTGCTTTAG AGGACTCCAA CCCAGAGAGA 
ATGCTCAGAA AGTTTTTTGA AGGACCACCT GAAAAGGGTT TTCAGTCCAT GAAACATAAT 
TCAACCAATG GTGGATTGCC TGAAATGGAT GATGAGGATT ACCACGACAT GGGTGTTAAA 
GTAGATGTCT ATGGAGATTT TGAAGAGGAG GACCCTTATA ACCCCTGCTG CCCTGAGCAA 
ATTTATGACA CAGTGGAGCC TCATTCCAGT CCTGAAGTGA TGAACCGTCC ACCCGCACCC 
ATCCCTCGAC CAGCCAAACT GCCCGAGCCT GAAGAAAACA CAACCTACAT TTCTAAAGTA 
TTTTGCCCCA AGGACCCTCT GTACTCAGTA AGCCAGCGAC CAGAAAGGGA TCCCTCTATA 
GCACCTGTGC GGCTGGTGAG AGACAGAGAC ATGAGCAGTC ACCATGACCC ATTCGCGGGC 
ATGAAGACCC CTGGACAGAG GCAGCTCATC TCTCTGCAGG AGAGGGTGAA GGTTGGAGAG 
CTGACTGTTG AAGAGGCCGT GCAGGAGTTT AAGGCTTGGC AGTTTGACCA GGATAAAAGA 
TGGCGCTCTC TACGCTTTCA ACAAGAAAAT TTACACCGAT TAAGAGAAAG CATCACCCGA 
CGCTGTAAAG ACAAAGGAAC AAATGAGCTC ATGATTACAG CTCCGATGCA AACGAACACT 
CAGTGGGGAT CTCAAATGAA TGTGAACTGC TCAGTTTACG AACCCACACC TAGGACGGTT 
GTGCAACCCC CTTCTGTCAA TCGAGCACCG CAGAGGGGAA GCTGGCAGAC AGGAAGCACC 
TCCAGCACTT CTAGCAGTAG CAGTCACAGA TTGAGCATTC AGAGCACAGT CAGCAACAGC 
AGTGGTGTGG ACGGTGAAGA GGAGCCTCCA GACATTCCTC TTCCTCCACG CCCCCTGCGG 
CCTGTAGTGG ACTCTCCTCC AATCCTTCCT CCACCCAGAG TCCCTCCACG AGTGATAGAA 
AGGAGCCCAG AGATACTGAA CGAGCGTTAC GTATCCAATC CATCCCGCCC TGTTCCTCAG 
AATCCTGCAC AAAGACCTAT ACCACCTCCG CCGGTACCCA GACGGACATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pik3ap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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