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kcnt1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol kcnt1
Entrez Gene ID 100004419
Full Name potassium channel, subfamily T, member 1
General protein information
Preferred Names

potassium channel, subfamily T, member 1

Names

potassium channel subfamily T member 1
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_009295129.2 XP_009293404.1 potassium channel subfamily T member 1 isoform X2
XM_009295129.3 XP_009293404.1 potassium channel subfamily T member 1 isoform X2
XM_009295130.3 XP_009293405.1 potassium channel subfamily T member 1 isoform X3
XM_009295128.2 XP_009293403.1 potassium channel subfamily T member 1 isoform X1
XM_009295130.2 XP_009293405.1 potassium channel subfamily T member 1 isoform X3
XM_009295128.3 XP_009293403.1 potassium channel subfamily T member 1 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) KCNT1 NP_065873.2
Caenorhabditis elegans (roundworm) slo-2 NP_001129924.1
Xenopus tropicalis (tropical clawed frog) kcnt2 NP_001163984.2
LOC721830 XP_001118025.2
Mus musculus (house mouse) Kcnt1 NP_780671.2
Pan troglodytes (chimpanzee) KCNT1 XP_003312453.2
Canis lupus familiaris (dog) KCNT1 XP_005625191.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007585 XP_001687885.2
Drosophila melanogaster (fruit fly) CG42732 NP_001097259.2
Bos taurus (cattle) KCNT1 XP_002691660.3
Rattus norvegicus (Norway rat) Kcnt1 NP_068625.1
Gallus gallus (chicken) KCNT1 NP_989893.1
Danio rerio (zebrafish) kcnt1 XP_001920632.3

Gene Ontology

The following kcnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa47918 XM_009295128.3
Latest version!		 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
ODa47919 XM_009295129.3
Latest version!		 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
ODa47920 XM_009295130.3
Latest version!		 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa47919 XM_009295129.2 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
ODa47920 XM_009295130.2 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $629.30
$899.00
ODa47918 XM_009295128.2 Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa47918
Clone ID Related Accession (Same CDS sequence) XM_009295128.2 , XM_009295128.3
Accession Version XM_009295128.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product potassium channel subfamily T member 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAAAGATG TCACAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTTAA TTTGGGCTAT TTGCAAGATG AGTTGAATGA CCAGCAGAAC 
TCCATCTCAT ATGTGCTCAT AAACCCCGCC CCCGACACAC TGCTGCAGCT CAATGATGTC 
GTGTTTTTGA TTCGTCCGGA TCCACTGGCT CACGTACCTG AAGCTCCGCC TGGACAGAAG 
CGCCGCTGCC GCTCCACCAC AGACACACCA GAACTCAACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293403.1
CDS215..3736
Translation

Target ORF information:

RefSeq Version XM_009295128.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295128.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAAAGATG TCACAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTTAA TTTGGGCTAT TTGCAAGATG AGTTGAATGA CCAGCAGAAC 
TCCATCTCAT ATGTGCTCAT AAACCCCGCC CCCGACACAC TGCTGCAGCT CAATGATGTC 
GTGTTTTTGA TTCGTCCGGA TCCACTGGCT CACGTACCTG AAGCTCCGCC TGGACAGAAG 
CGCCGCTGCC GCTCCACCAC AGACACACCA GAACTCAACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa47918
Clone ID Related Accession (Same CDS sequence) XM_009295128.2 , XM_009295128.3
Accession Version XM_009295128.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product potassium channel subfamily T member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAAAGATG TCACAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTTAA TTTGGGCTAT TTGCAAGATG AGTTGAATGA CCAGCAGAAC 
TCCATCTCAT ATGTGCTCAT AAACCCCGCC CCCGACACAC TGCTGCAGCT CAATGATGTC 
GTGTTTTTGA TTCGTCCGGA TCCACTGGCT CACGTACCTG AAGCTCCGCC TGGACAGAAG 
CGCCGCTGCC GCTCCACCAC AGACACACCA GAACTCAACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293403.1
CDS196..3717
Translation

Target ORF information:

RefSeq Version XM_009295128.3
Organism Danio rerio(zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295128.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAAAGATG TCACAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTTAA TTTGGGCTAT TTGCAAGATG AGTTGAATGA CCAGCAGAAC 
TCCATCTCAT ATGTGCTCAT AAACCCCGCC CCCGACACAC TGCTGCAGCT CAATGATGTC 
GTGTTTTTGA TTCGTCCGGA TCCACTGGCT CACGTACCTG AAGCTCCGCC TGGACAGAAG 
CGCCGCTGCC GCTCCACCAC AGACACACCA GAACTCAACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa47919
Clone ID Related Accession (Same CDS sequence) XM_009295129.2 , XM_009295129.3
Accession Version XM_009295129.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product potassium channel subfamily T member 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAATGTCA CAAATTTAAC AGCCAGTGAT 
GTGATGAATC GGGTTAATTT GGGCTATTTG CAAGATGAGT TGAATGACCA GCAGAACTCC 
ATCTCATATG TGCTCATAAA CCCCGCCCCC GACACACTGC TGCAGCTCAA TGATGTCGTG 
TTTTTGATTC GTCCGGATCC ACTGGCTCAC GTACCTGAAG CTCCGCCTGG ACAGAAGCGC 
CGCTGCCGCT CCACCACAGA CACACCAGAA CTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293404.1
CDS215..3733
Translation

Target ORF information:

RefSeq Version XM_009295129.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295129.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAATGTCA CAAATTTAAC AGCCAGTGAT 
GTGATGAATC GGGTTAATTT GGGCTATTTG CAAGATGAGT TGAATGACCA GCAGAACTCC 
ATCTCATATG TGCTCATAAA CCCCGCCCCC GACACACTGC TGCAGCTCAA TGATGTCGTG 
TTTTTGATTC GTCCGGATCC ACTGGCTCAC GTACCTGAAG CTCCGCCTGG ACAGAAGCGC 
CGCTGCCGCT CCACCACAGA CACACCAGAA CTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa47919
Clone ID Related Accession (Same CDS sequence) XM_009295129.2 , XM_009295129.3
Accession Version XM_009295129.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product potassium channel subfamily T member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAATGTCA CAAATTTAAC AGCCAGTGAT 
GTGATGAATC GGGTTAATTT GGGCTATTTG CAAGATGAGT TGAATGACCA GCAGAACTCC 
ATCTCATATG TGCTCATAAA CCCCGCCCCC GACACACTGC TGCAGCTCAA TGATGTCGTG 
TTTTTGATTC GTCCGGATCC ACTGGCTCAC GTACCTGAAG CTCCGCCTGG ACAGAAGCGC 
CGCTGCCGCT CCACCACAGA CACACCAGAA CTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293404.1
CDS196..3714
Translation

Target ORF information:

RefSeq Version XM_009295129.3
Organism Danio rerio(zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295129.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GTTTTGTAAT 
TGGAATATTG CCAAATGTGT GTTCGATCAA CAGAAGGAAC GTGACAAAGG CTCAAATCTG 
GCCTTCATGT TTCGCCTTCC CTTTGCTGCT GGCCGAGTCT TCAGTATCAG CATGCTGGAC 
ACACTCCTGT ACCAGTGTTT TGTGAAGGAC TACATGATCC CCATAGCCAG ATTACTGCTG 
GGATTGGACA CCACACCTGG ATCTGGATAC CTGTGTGCTA TGCACATATG TGAGGCTGAT 
CTCTGGATCC GCACATACGG GAGACTCTTC CAGAAGTTCT GCTCATCCAG TTCAGAGATT 
CCCATCGGAA TCTACCGCAC AGAATCACAC GCACCACCTG AGCTTCAAAG CTCAGTCAGC 
ATAGACGGTT TAAACGACAC TAGAGAGAAA GGCGAGGAGT CAAAGATCCT CACCCGGAGC 
TCCTCAAGCA GCGACCAATC AGATCACCCA CTGCTGAGAA AGAAGAGCAT GCATTGGACA 
AGACGTCTCA GTAGGCGGAG TGTAAAGCGG AGCGACTCCT CCCTCAGCTC CATCCAGCAG 
CGTCTGGGTG TGTTACGGCG CTCTGAGAGG GAGGAGCTTA CTGAACTGGT CAGAAACCGC 
ATGCAGCATT TAGGCCTGCA CACTGGCGGA TACAATGTCA CAAATTTAAC AGCCAGTGAT 
GTGATGAATC GGGTTAATTT GGGCTATTTG CAAGATGAGT TGAATGACCA GCAGAACTCC 
ATCTCATATG TGCTCATAAA CCCCGCCCCC GACACACTGC TGCAGCTCAA TGATGTCGTG 
TTTTTGATTC GTCCGGATCC ACTGGCTCAC GTACCTGAAG CTCCGCCTGG ACAGAAGCGC 
CGCTGCCGCT CCACCACAGA CACACCAGAA CTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa47920
Clone ID Related Accession (Same CDS sequence) XM_009295130.3 , XM_009295130.2
Accession Version XM_009295130.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product potassium channel subfamily T member 1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GAAGGAACGT 
GACAAAGGCT CAAATCTGGC CTTCATGTTT CGCCTTCCCT TTGCTGCTGG CCGAGTCTTC 
AGTATCAGCA TGCTGGACAC ACTCCTGTAC CAGTGTTTTG TGAAGGACTA CATGATCCCC 
ATAGCCAGAT TACTGCTGGG ATTGGACACC ACACCTGGAT CTGGATACCT GTGTGCTATG 
CACATATGTG AGGCTGATCT CTGGATCCGC ACATACGGGA GACTCTTCCA GAAGTTCTGC 
TCATCCAGTT CAGAGATTCC CATCGGAATC TACCGCACAG AATCACACGC ACCACCTGAG 
CTTCAAAGCT CAGTCAGCAT AGACGGTTTA AACGACACTA GAGAGAAAGG CGAGGAGTCA 
AAGATCCTCA CCCGGAGCTC CTCAAGCAGC GACCAATCAG ATCACCCACT GCTGAGAAAG 
AAGAGCATGC ATTGGACAAG ACGTCTCAGT AGGCGGAGTG TAAAGCGGAG CGACTCCTCC 
CTCAGCTCCA TCCAGCAGCG TCTGGGTGTG TTACGGCGCT CTGAGAGGGA GGAGCTTACT 
GAACTGGTCA GAAACCGCAT GCAGCATTTA GGCCTGCACA CTGGCGGATA CAAAGATGTC 
ACAAATTTAA CAGCCAGTGA TGTGATGAAT CGGGTTAATT TGGGCTATTT GCAAGATGAG 
TTGAATGACC AGCAGAACTC CATCTCATAT GTGCTCATAA ACCCCGCCCC CGACACACTG 
CTGCAGCTCA ATGATGTCGT GTTTTTGATT CGTCCGGATC CACTGGCTCA CGTACCTGAA 
GCTCCGCCTG GACAGAAGCG CCGCTGCCGC TCCACCACAG ACACACCAGA ACTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293405.1
CDS195..3674
Translation

Target ORF information:

RefSeq Version XM_009295130.3
Organism Danio rerio(zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295130.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GAAGGAACGT 
GACAAAGGCT CAAATCTGGC CTTCATGTTT CGCCTTCCCT TTGCTGCTGG CCGAGTCTTC 
AGTATCAGCA TGCTGGACAC ACTCCTGTAC CAGTGTTTTG TGAAGGACTA CATGATCCCC 
ATAGCCAGAT TACTGCTGGG ATTGGACACC ACACCTGGAT CTGGATACCT GTGTGCTATG 
CACATATGTG AGGCTGATCT CTGGATCCGC ACATACGGGA GACTCTTCCA GAAGTTCTGC 
TCATCCAGTT CAGAGATTCC CATCGGAATC TACCGCACAG AATCACACGC ACCACCTGAG 
CTTCAAAGCT CAGTCAGCAT AGACGGTTTA AACGACACTA GAGAGAAAGG CGAGGAGTCA 
AAGATCCTCA CCCGGAGCTC CTCAAGCAGC GACCAATCAG ATCACCCACT GCTGAGAAAG 
AAGAGCATGC ATTGGACAAG ACGTCTCAGT AGGCGGAGTG TAAAGCGGAG CGACTCCTCC 
CTCAGCTCCA TCCAGCAGCG TCTGGGTGTG TTACGGCGCT CTGAGAGGGA GGAGCTTACT 
GAACTGGTCA GAAACCGCAT GCAGCATTTA GGCCTGCACA CTGGCGGATA CAAAGATGTC 
ACAAATTTAA CAGCCAGTGA TGTGATGAAT CGGGTTAATT TGGGCTATTT GCAAGATGAG 
TTGAATGACC AGCAGAACTC CATCTCATAT GTGCTCATAA ACCCCGCCCC CGACACACTG 
CTGCAGCTCA ATGATGTCGT GTTTTTGATT CGTCCGGATC CACTGGCTCA CGTACCTGAA 
GCTCCGCCTG GACAGAAGCG CCGCTGCCGC TCCACCACAG ACACACCAGA ACTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa47920
Clone ID Related Accession (Same CDS sequence) XM_009295130.3 , XM_009295130.2
Accession Version XM_009295130.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product potassium channel subfamily T member 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GAAGGAACGT 
GACAAAGGCT CAAATCTGGC CTTCATGTTT CGCCTTCCCT TTGCTGCTGG CCGAGTCTTC 
AGTATCAGCA TGCTGGACAC ACTCCTGTAC CAGTGTTTTG TGAAGGACTA CATGATCCCC 
ATAGCCAGAT TACTGCTGGG ATTGGACACC ACACCTGGAT CTGGATACCT GTGTGCTATG 
CACATATGTG AGGCTGATCT CTGGATCCGC ACATACGGGA GACTCTTCCA GAAGTTCTGC 
TCATCCAGTT CAGAGATTCC CATCGGAATC TACCGCACAG AATCACACGC ACCACCTGAG 
CTTCAAAGCT CAGTCAGCAT AGACGGTTTA AACGACACTA GAGAGAAAGG CGAGGAGTCA 
AAGATCCTCA CCCGGAGCTC CTCAAGCAGC GACCAATCAG ATCACCCACT GCTGAGAAAG 
AAGAGCATGC ATTGGACAAG ACGTCTCAGT AGGCGGAGTG TAAAGCGGAG CGACTCCTCC 
CTCAGCTCCA TCCAGCAGCG TCTGGGTGTG TTACGGCGCT CTGAGAGGGA GGAGCTTACT 
GAACTGGTCA GAAACCGCAT GCAGCATTTA GGCCTGCACA CTGGCGGATA CAAAGATGTC 
ACAAATTTAA CAGCCAGTGA TGTGATGAAT CGGGTTAATT TGGGCTATTT GCAAGATGAG 
TTGAATGACC AGCAGAACTC CATCTCATAT GTGCTCATAA ACCCCGCCCC CGACACACTG 
CTGCAGCTCA ATGATGTCGT GTTTTTGATT CGTCCGGATC CACTGGCTCA CGTACCTGAA 
GCTCCGCCTG GACAGAAGCG CCGCTGCCGC TCCACCACAG ACACACCAGA ACTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009293405.1
CDS215..3694
Translation

Target ORF information:

RefSeq Version XM_009295130.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio potassium channel, subfamily T, member 1 (kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009295130.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAACAGA AGAATGCAGT GCCATCCTGG GTTAACCCGG CTCTGGTCAA CTGGAGGCTG 
GACTCTTTCG GTAGTGATGC TGGGCAGAGG GTTCATGTGG AGTTTTATGT GGACGAGAAC 
ACATTTAAAG AGAGACTTAA ACTTTTTTTC ATCAAAAACC AGAGATCCAG TTTAAGGATC 
CGAATATTCA ACTTCTGCCT GAAGCTTTTG ACATGTGTTC TCTACATCAT ACGGGTGATG 
ACGGACAATC CAGCCCAGCT TCGCCACACA TGTGTGAACT GCAGCTCACA GAACGACATC 
AACTGGGAGC TGATATTCTG GGTTGACAGA AAGGTACCGG TGTGGGCCAT TCAGGTGATT 
GTAGCGTCCA TCAGTTTTAT GGAGGCCATG TTACTGACCT ACCTGAGCTA CAAGGGTAAT 
TTATGGGAGC AGATCTTTCA GGTGTCTTTC CTGCTGGAGA TGCTCAACAC CGTCCCGTTC 
ATCATCACGA TCTTCTGGCC TCCTCTCAGA AACCTCTTCA TCCCAGTGTT CCTCAACTGC 
TGGCTGGCCA AATGTGCTCT GGAGAGCATG ATAAATGATC TGCACAGAGC CATTCAGAGG 
ACTCACTCCG CCATGTTTAA TCAGGTCCTG ATCCTCATCT GCACCCTCCT GTGTCTCGTC 
TTTACCGGCA CGTGTGGGAT CCAGCATTTG GAGCGGGCAG GTAAGAAGAG TCTGTCGCTC 
TTCAACGCTC TGTATTTCTG CATCGTGACC TTCTCAACGG TGGGTTTCGG GGACGTAACG 
CCCCAGATCT GGCCTTCTCA GCTGCTGGTG GTGGTGATGA TCTGCGTGGC CCTCGTCGTG 
CTGCCGCTTC AGTTTGAGGA GTTGATTTAT CTGTGGATGG AAAGACAGAA ATCTGGAGGA 
AACTACAGCA GGCATCGCGC TCAGACAGAG AGACACGTGG TTTTATGTGT CAGTACTCTG 
AAAATCGACC TGCTGATGGA CTTTCTCAAT GAGTTCTACG CTCACCCCCA CACACAGGAT 
TATTATGTGG TCATCTTGTG TCCGTGTGAA GTGGACGTTC AGGTCAGGAG GATTTTACAG 
ATTCCTCTCT GGTCACAGAG GGTCATTTAT CTTCAAGGCT CCGCCCTCAA AAACCAGGAT 
TTGCTGAGAG CCAAGATGGA CGATGCGGAG GCATGTTTTA TTCTTAGCAG CAGGAATGAA 
GCGGATCGCA TGGCAGCAGA TCACCAGACC ATTCTGAGGG CTTGGGCTGT GAAAGACTTC 
GCTCCAAACT GCCCTCTGTA TGTCCAGATC CTTAAACCCG AAAACAAATT CCATGTTAAA 
TTTGCAGATC ACGTTGTGTG TGAGGAAGAG TTTAAATACG CCATGTTGGC TCTGAACTGT 
CTGTGTCCGG CCACTTCTAC TCTGATCACT TTACTGGTTC ACACATCTCG CGGACAAGAG 
GGCCAGCAGT CTCCGGAGCA GTGGCAGAGG ATGTATGGTC GATGTTCGGG TAATGAGGTC 
TATCATATCC GCCTGGGTGA CAGTATGTTC TTCAGGGAGT ACAACAGCAA AAGCTTTACC 
TATGCTGCCT TTCACGCTCA CAAAAAGTAC GGTGTGTGTA TGATTGGTGT GAAGAGAGAA 
GATAATAAGA GCATCTTGTT GAACCCTGGA CCGCGACACA TCATGTCTGC CAGCGACACC 
TGTTACTACA TCAACATCAC GAAAGAGGAA AACTCCGCCT TCATCTTCAG ACAGGAGGAA 
GACCAGGGAA AGGGGCGGAG CCACTGCGAC TTTTTAAACA GCCCGTCAGG ACTACCTGTT 
CATAGCATTA TTGCTAGCAT GGGAACGGTT GCCATGGATT TTCAGAACAC CAGTCCCACT 
GAGAGCGGAC TCAAACTGGC TCCGCCCACA GAGAACGAAC TGGGAAGTCG ACGACCAAGT 
ATAGCGCCCG TTTTGGAGAT TGCTGACTCC ACCTCCTTAC TGCCGTGTGA CCTGCTGAGC 
GACCAATCAG AGGACGAGGC CGGACACTCG AATGAAGATA TTACAGCCAC TGATTTTGTG 
AAAGGTTATC CCCCGAACTC TCCATATATT GGGAGCTCTC CAACCCTTTG CCATCTTTTA 
CCTCAGAAAG CCCCTTTCTG CTGTTTACGG CTTGACCAGA GCTGTGAGCA TGTTTCCTTT 
GAAGACGCAA AAGCATACGG CTTCAAAAAT AAATTAATTA TCGTTTCTGC GGAGACGGCT 
GGAAACGGCC TTTACAACTT CATAGTGCCT TTAAGAGCCT ATTACAGGCC CAGAAGAGAA 
CTCAACCCTA TAGTCCTGCT GTTAGACAAC CTACCCGATG ACCACTTCCT TGAGGCCATA 
TCCTGCTTCC CAATGGTGTA TTATATGGGT GGCACCATTG ACAACTTGGA CAGCCTATTG 
CAGTGTGGCG TTCTGTATGC TGATAATCTA GTGGTTGTTG ACAAAGAAAG CACGATGAGT 
GCAGAGGAAG ACTATATGGC TGACGCTAAA ACCATCGTCA ATGTGCAAAC CATGTTCAGG 
TTGTTTCCGA GTCTCAGTAT AATCACAGAA CTGACTCACC CGTCCAACAT GAGATTCATG 
CAGTTCAGAG CGAAGGACTG CTACTCGCTC GCCCTGTCCA AACTAGAGAA GAAGGAACGT 
GACAAAGGCT CAAATCTGGC CTTCATGTTT CGCCTTCCCT TTGCTGCTGG CCGAGTCTTC 
AGTATCAGCA TGCTGGACAC ACTCCTGTAC CAGTGTTTTG TGAAGGACTA CATGATCCCC 
ATAGCCAGAT TACTGCTGGG ATTGGACACC ACACCTGGAT CTGGATACCT GTGTGCTATG 
CACATATGTG AGGCTGATCT CTGGATCCGC ACATACGGGA GACTCTTCCA GAAGTTCTGC 
TCATCCAGTT CAGAGATTCC CATCGGAATC TACCGCACAG AATCACACGC ACCACCTGAG 
CTTCAAAGCT CAGTCAGCAT AGACGGTTTA AACGACACTA GAGAGAAAGG CGAGGAGTCA 
AAGATCCTCA CCCGGAGCTC CTCAAGCAGC GACCAATCAG ATCACCCACT GCTGAGAAAG 
AAGAGCATGC ATTGGACAAG ACGTCTCAGT AGGCGGAGTG TAAAGCGGAG CGACTCCTCC 
CTCAGCTCCA TCCAGCAGCG TCTGGGTGTG TTACGGCGCT CTGAGAGGGA GGAGCTTACT 
GAACTGGTCA GAAACCGCAT GCAGCATTTA GGCCTGCACA CTGGCGGATA CAAAGATGTC 
ACAAATTTAA CAGCCAGTGA TGTGATGAAT CGGGTTAATT TGGGCTATTT GCAAGATGAG 
TTGAATGACC AGCAGAACTC CATCTCATAT GTGCTCATAA ACCCCGCCCC CGACACACTG 
CTGCAGCTCA ATGATGTCGT GTTTTTGATT CGTCCGGATC CACTGGCTCA CGTACCTGAA 
GCTCCGCCTG GACAGAAGCG CCGCTGCCGC TCCACCACAG ACACACCAGA ACTCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed