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CHRM4A cDNA ORF clone, Danio rerio(Zebrafish)

Summary

Gene Symbol CHRM4A
Entrez Gene ID 100150701
Full Name cholinergic receptor, muscarinic 4a
General protein information
Preferred Names

cholinergic receptor, muscarinic 4a

Names

muscarinic acetylcholine receptor M4
DrM4A
Gene Type protein-coding
Organism Danio rerio(Zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_001922407.5 XP_001922442.1 muscarinic acetylcholine receptor M4
XM_001922407.4 XP_001922442.1 muscarinic acetylcholine receptor M4

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) CHRM4 XP_005631350.1
Rattus norvegicus (Norway rat) Chrm4 NP_113735.1
Gallus gallus (chicken) CHRM4 NP_001026362.1
Homo sapiens (human) CHRM4 NP_000732.2
Mus musculus (house mouse) Chrm4 NP_031725.1
Danio rerio (zebrafish) chrm4a XP_001922442.1
CHRM4 XP_001112370.1
Xenopus tropicalis (tropical clawed frog) chrm4 NP_001106514.1

Gene Ontology

The following CHRM4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRM4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13287 XM_001922407.5
Latest version!		 Danio rerio cholinergic receptor, muscarinic 4a (chrm4a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa13287 XM_001922407.4 Danio rerio cholinergic receptor, muscarinic 4a (chrm4a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa13287
Clone ID Related Accession (Same CDS sequence) XM_001922407.5 , XM_001922407.4
Accession Version XM_001922407.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product muscarinic acetylcholine receptor M4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922407.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGTAA CTAACAGCAC TGGAGCAGAG GGACTCGCGC CCTGGAACTT CAACGGCTCT 
TTGAGCAACG TGTCCAGTGA TGCCAACTTC AGCTGTGACA GTGGCAGCGC CAATGGCTCA 
TGTGCAGCGG TTGAGGGCGA CGGCGAGAGC CCGTACAAGA CGGTGGAGAT GGTCTTCATC 
GCACTGGTCA CTGGATCTCT CAGTTTCGTC ACCGTCGTGG GCAACATCCT CGTCATGCTC 
TCAATCAAGG TTAATCGACA CCTGCAGACG GTCAACAACT ACTTCCTTTT CAGCCTGGCC 
TGTGCAGATC TGATCATCGG TGTGTTCTCC ATGAACCTTT ACACCGTCTA CATCATTAAG 
GGTTACTGGC CGCTTGGCCC AGTGGTGTGT GACCTGTGGC TCGCGCTGGA TTACGTGGTC 
AGCAACGCTT CAGTCATGAA CCTGCTAATC ATCAGCTTCG ACCGATACTT CTGTGTGACC 
AAACCCTTGA GCTACCCCAC ACGCAGAACC ACCAAGATGG CCGGCCTCAT GATTGCCTCT 
GCCTGGATCC TGTCCTTCAT CCTGTGGGCT CCCGCTATCC TGTTCTGGCA GTTTATCGTC 
GGAGCCCGAA CCGTAGCACC TGGGGAATGT TACATCCAGT TCCTTTCCAA CCCCGCGGTC 
ACATTTGGCA CAGCCATCGC TGCGTTCTAC CTTCCGGTGG TCATCATGAC AGTCCTTTAT 
GTGCACATCT CTCTGGCAAG CCGCAGTCGT GTGTCCAAAC AGAAGCCTGA AGCCAAGAAA 
GAGAAGAAAG CCCAGAAATC TAGCGGCCTG CTCAAGAGTC ACATCCTAAA GCAGAACAAC 
AATAACCAGT CTCCTCCTAA GCCCAGCCTG GACACCTGCT CTACAGTGGA CACCATGAAA 
AACGGTAAAC TGGAAGAGTC TGTGGTGTCC GCTAAAGCAG ACTCTTGCGT GCAGGCGGAG 
GAAAAGGAAA GCTCCAATGA TTCCAGCACA GCCAGCATAG CGCCAAAGGA GCCCAAAGAG 
CGAGCGAACA GTGAGGTCAC TTCAGACGCA GGTCTGACCC CTGCGCCAGC AGCTGCACCT 
AAACTCAACC CTCAATCCAA ATGGTCCAAG ATCAAGATAG TCACCAAACA GGCAGGAGAT 
GAGTGCATCA CAGCAATTGA GATCGTCCCA CCGAACAGCA CCGGCGAGAG CCGCTCCATC 
CCTGTGAGCC GTCCCCGCAC TGTGGCGCGC AAGTTTGCCA GCATTGCACG CAGTCAGGTG 
AAGAGGAAAC GGCAAATGGC GGCACGAGAA AAGAAAGTGA CAAAGACAAT CTTTGCCATC 
CTGCTGGCCT TTATCATCAC ATGGACGCCG TACAATGTAA TGGTTTTAAT TAGCACGTTC 
TGCCAGTCGT GTGTTCCAGA CACAGTCTGG GCCATTGGCT ACTGGCTCTG CTACGTCAAC 
AGCACCATCA ACCCCGCTTG CTATGCACTA TGTAACGCCA CTTTCAAAAA GACCTTCAAA 
AACCTGCTCA TGTGCCAGTA TAAGAACATC GGCACCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_001922442.1
CDS589..2130
Translation

Target ORF information:

RefSeq Version XM_001922407.5
Organism Danio rerio(zebrafish)
Definition Danio rerio cholinergic receptor, muscarinic 4a (chrm4a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_001922407.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGTAA CTAACAGCAC TGGAGCAGAG GGACTCGCGC CCTGGAACTT CAACGGCTCT 
TTGAGCAACG TGTCCAGTGA TGCCAACTTC AGCTGTGACA GTGGCAGCGC CAATGGCTCA 
TGTGCAGCGG TTGAGGGCGA CGGCGAGAGC CCGTACAAGA CGGTGGAGAT GGTCTTCATC 
GCACTGGTCA CTGGATCTCT CAGTTTCGTC ACCGTCGTGG GCAACATCCT CGTCATGCTC 
TCAATCAAGG TTAATCGACA CCTGCAGACG GTCAACAACT ACTTCCTTTT CAGCCTGGCC 
TGTGCAGATC TGATCATCGG TGTGTTCTCC ATGAACCTTT ACACCGTCTA CATCATTAAG 
GGTTACTGGC CGCTTGGCCC AGTGGTGTGT GACCTGTGGC TCGCGCTGGA TTACGTGGTC 
AGCAACGCTT CAGTCATGAA CCTGCTAATC ATCAGCTTCG ACCGATACTT CTGTGTGACC 
AAACCCTTGA GCTACCCCAC ACGCAGAACC ACCAAGATGG CCGGCCTCAT GATTGCCTCT 
GCCTGGATCC TGTCCTTCAT CCTGTGGGCT CCCGCTATCC TGTTCTGGCA GTTTATCGTC 
GGAGCCCGAA CCGTAGCACC TGGGGAATGT TACATCCAGT TCCTTTCCAA CCCCGCGGTC 
ACATTTGGCA CAGCCATCGC TGCGTTCTAC CTTCCGGTGG TCATCATGAC AGTCCTTTAT 
GTGCACATCT CTCTGGCAAG CCGCAGTCGT GTGTCCAAAC AGAAGCCTGA AGCCAAGAAA 
GAGAAGAAAG CCCAGAAATC TAGCGGCCTG CTCAAGAGTC ACATCCTAAA GCAGAACAAC 
AATAACCAGT CTCCTCCTAA GCCCAGCCTG GACACCTGCT CTACAGTGGA CACCATGAAA 
AACGGTAAAC TGGAAGAGTC TGTGGTGTCC GCTAAAGCAG ACTCTTGCGT GCAGGCGGAG 
GAAAAGGAAA GCTCCAATGA TTCCAGCACA GCCAGCATAG CGCCAAAGGA GCCCAAAGAG 
CGAGCGAACA GTGAGGTCAC TTCAGACGCA GGTCTGACCC CTGCGCCAGC AGCTGCACCT 
AAACTCAACC CTCAATCCAA ATGGTCCAAG ATCAAGATAG TCACCAAACA GGCAGGAGAT 
GAGTGCATCA CAGCAATTGA GATCGTCCCA CCGAACAGCA CCGGCGAGAG CCGCTCCATC 
CCTGTGAGCC GTCCCCGCAC TGTGGCGCGC AAGTTTGCCA GCATTGCACG CAGTCAGGTG 
AAGAGGAAAC GGCAAATGGC GGCACGAGAA AAGAAAGTGA CAAAGACAAT CTTTGCCATC 
CTGCTGGCCT TTATCATCAC ATGGACGCCG TACAATGTAA TGGTTTTAAT TAGCACGTTC 
TGCCAGTCGT GTGTTCCAGA CACAGTCTGG GCCATTGGCT ACTGGCTCTG CTACGTCAAC 
AGCACCATCA ACCCCGCTTG CTATGCACTA TGTAACGCCA CTTTCAAAAA GACCTTCAAA 
AACCTGCTCA TGTGCCAGTA TAAGAACATC GGCACCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa13287
Clone ID Related Accession (Same CDS sequence) XM_001922407.5 , XM_001922407.4
Accession Version XM_001922407.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product muscarinic acetylcholine receptor M4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922407.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGTAA CTAACAGCAC TGGAGCAGAG GGACTCGCGC CCTGGAACTT CAACGGCTCT 
TTGAGCAACG TGTCCAGTGA TGCCAACTTC AGCTGTGACA GTGGCAGCGC CAATGGCTCA 
TGTGCAGCGG TTGAGGGCGA CGGCGAGAGC CCGTACAAGA CGGTGGAGAT GGTCTTCATC 
GCACTGGTCA CTGGATCTCT CAGTTTCGTC ACCGTCGTGG GCAACATCCT CGTCATGCTC 
TCAATCAAGG TTAATCGACA CCTGCAGACG GTCAACAACT ACTTCCTTTT CAGCCTGGCC 
TGTGCAGATC TGATCATCGG TGTGTTCTCC ATGAACCTTT ACACCGTCTA CATCATTAAG 
GGTTACTGGC CGCTTGGCCC AGTGGTGTGT GACCTGTGGC TCGCGCTGGA TTACGTGGTC 
AGCAACGCTT CAGTCATGAA CCTGCTAATC ATCAGCTTCG ACCGATACTT CTGTGTGACC 
AAACCCTTGA GCTACCCCAC ACGCAGAACC ACCAAGATGG CCGGCCTCAT GATTGCCTCT 
GCCTGGATCC TGTCCTTCAT CCTGTGGGCT CCCGCTATCC TGTTCTGGCA GTTTATCGTC 
GGAGCCCGAA CCGTAGCACC TGGGGAATGT TACATCCAGT TCCTTTCCAA CCCCGCGGTC 
ACATTTGGCA CAGCCATCGC TGCGTTCTAC CTTCCGGTGG TCATCATGAC AGTCCTTTAT 
GTGCACATCT CTCTGGCAAG CCGCAGTCGT GTGTCCAAAC AGAAGCCTGA AGCCAAGAAA 
GAGAAGAAAG CCCAGAAATC TAGCGGCCTG CTCAAGAGTC ACATCCTAAA GCAGAACAAC 
AATAACCAGT CTCCTCCTAA GCCCAGCCTG GACACCTGCT CTACAGTGGA CACCATGAAA 
AACGGTAAAC TGGAAGAGTC TGTGGTGTCC GCTAAAGCAG ACTCTTGCGT GCAGGCGGAG 
GAAAAGGAAA GCTCCAATGA TTCCAGCACA GCCAGCATAG CGCCAAAGGA GCCCAAAGAG 
CGAGCGAACA GTGAGGTCAC TTCAGACGCA GGTCTGACCC CTGCGCCAGC AGCTGCACCT 
AAACTCAACC CTCAATCCAA ATGGTCCAAG ATCAAGATAG TCACCAAACA GGCAGGAGAT 
GAGTGCATCA CAGCAATTGA GATCGTCCCA CCGAACAGCA CCGGCGAGAG CCGCTCCATC 
CCTGTGAGCC GTCCCCGCAC TGTGGCGCGC AAGTTTGCCA GCATTGCACG CAGTCAGGTG 
AAGAGGAAAC GGCAAATGGC GGCACGAGAA AAGAAAGTGA CAAAGACAAT CTTTGCCATC 
CTGCTGGCCT TTATCATCAC ATGGACGCCG TACAATGTAA TGGTTTTAAT TAGCACGTTC 
TGCCAGTCGT GTGTTCCAGA CACAGTCTGG GCCATTGGCT ACTGGCTCTG CTACGTCAAC 
AGCACCATCA ACCCCGCTTG CTATGCACTA TGTAACGCCA CTTTCAAAAA GACCTTCAAA 
AACCTGCTCA TGTGCCAGTA TAAGAACATC GGCACCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_001922442.1
CDS559..2100
Translation

Target ORF information:

RefSeq Version XM_001922407.4
Organism Danio rerio(Zebrafish)
Definition Danio rerio cholinergic receptor, muscarinic 4a (chrm4a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_001922407.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGTAA CTAACAGCAC TGGAGCAGAG GGACTCGCGC CCTGGAACTT CAACGGCTCT 
TTGAGCAACG TGTCCAGTGA TGCCAACTTC AGCTGTGACA GTGGCAGCGC CAATGGCTCA 
TGTGCAGCGG TTGAGGGCGA CGGCGAGAGC CCGTACAAGA CGGTGGAGAT GGTCTTCATC 
GCACTGGTCA CTGGATCTCT CAGTTTCGTC ACCGTCGTGG GCAACATCCT CGTCATGCTC 
TCAATCAAGG TTAATCGACA CCTGCAGACG GTCAACAACT ACTTCCTTTT CAGCCTGGCC 
TGTGCAGATC TGATCATCGG TGTGTTCTCC ATGAACCTTT ACACCGTCTA CATCATTAAG 
GGTTACTGGC CGCTTGGCCC AGTGGTGTGT GACCTGTGGC TCGCGCTGGA TTACGTGGTC 
AGCAACGCTT CAGTCATGAA CCTGCTAATC ATCAGCTTCG ACCGATACTT CTGTGTGACC 
AAACCCTTGA GCTACCCCAC ACGCAGAACC ACCAAGATGG CCGGCCTCAT GATTGCCTCT 
GCCTGGATCC TGTCCTTCAT CCTGTGGGCT CCCGCTATCC TGTTCTGGCA GTTTATCGTC 
GGAGCCCGAA CCGTAGCACC TGGGGAATGT TACATCCAGT TCCTTTCCAA CCCCGCGGTC 
ACATTTGGCA CAGCCATCGC TGCGTTCTAC CTTCCGGTGG TCATCATGAC AGTCCTTTAT 
GTGCACATCT CTCTGGCAAG CCGCAGTCGT GTGTCCAAAC AGAAGCCTGA AGCCAAGAAA 
GAGAAGAAAG CCCAGAAATC TAGCGGCCTG CTCAAGAGTC ACATCCTAAA GCAGAACAAC 
AATAACCAGT CTCCTCCTAA GCCCAGCCTG GACACCTGCT CTACAGTGGA CACCATGAAA 
AACGGTAAAC TGGAAGAGTC TGTGGTGTCC GCTAAAGCAG ACTCTTGCGT GCAGGCGGAG 
GAAAAGGAAA GCTCCAATGA TTCCAGCACA GCCAGCATAG CGCCAAAGGA GCCCAAAGAG 
CGAGCGAACA GTGAGGTCAC TTCAGACGCA GGTCTGACCC CTGCGCCAGC AGCTGCACCT 
AAACTCAACC CTCAATCCAA ATGGTCCAAG ATCAAGATAG TCACCAAACA GGCAGGAGAT 
GAGTGCATCA CAGCAATTGA GATCGTCCCA CCGAACAGCA CCGGCGAGAG CCGCTCCATC 
CCTGTGAGCC GTCCCCGCAC TGTGGCGCGC AAGTTTGCCA GCATTGCACG CAGTCAGGTG 
AAGAGGAAAC GGCAAATGGC GGCACGAGAA AAGAAAGTGA CAAAGACAAT CTTTGCCATC 
CTGCTGGCCT TTATCATCAC ATGGACGCCG TACAATGTAA TGGTTTTAAT TAGCACGTTC 
TGCCAGTCGT GTGTTCCAGA CACAGTCTGG GCCATTGGCT ACTGGCTCTG CTACGTCAAC 
AGCACCATCA ACCCCGCTTG CTATGCACTA TGTAACGCCA CTTTCAAAAA GACCTTCAAA 
AACCTGCTCA TGTGCCAGTA TAAGAACATC GGCACCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed