KCNH1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Gene Symbol	KCNH1B
Entrez Gene ID	101154640
Full Name	potassium voltage-gated channel, subfamily H (eag-related), member 1b
Synonyms	Eag1,Kv10.1
General protein information	Preferred Names potassium voltage-gated channel, subfamily H (eag-related), member 1b Names potassium voltage-gated channel, subfamily H (eag-related), member 1b potassium voltage-gated channel subfamily H member 1b zmp:0000000565
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278814.1	NP_001265743.1	potassium voltage-gated channel, subfamily H (eag-related), member 1b
XM_009296521.3	XP_009294796.1	potassium voltage-gated channel, subfamily H (eag-related), member 1b isoform X1
XM_009296521.2	XP_009294796.1	potassium voltage-gated channel, subfamily H (eag-related), member 1b isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnh1	NP_113930.1
Xenopus tropicalis (tropical clawed frog)	kcnh1	XP_002934755.2
Canis lupus familiaris (dog)	LOC490277	XP_005623012.1
Mus musculus (house mouse)	Kcnh1	NP_001033696.1
Gallus gallus (chicken)	KCNH1	XP_419440.4
Homo sapiens (human)	KCNH1	NP_758872.1
Pan troglodytes (chimpanzee)	KCNH1	XP_001169423.2
	KCNH1	XP_001109251.1
Danio rerio (zebrafish)	kcnh1b	NP_001265743.1
Bos taurus (cattle)	KCNH1	NP_776797.1
Danio rerio (zebrafish)	kcnh1a	NP_001038396.1
Oryza sativa (rice)	Os01g0756700	NP_001044290.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Kcnh1 voltage-gated potassium channels are essential for early zebrafish development.
Stengel R, Rivera-Milla E, Sahoo N, Ebert C, Bollig F, Heinemann SH, Schönherr R, Englert C
The Journal of biological chemistry287(42)35565-75(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KCNH1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNH1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36778	NM_001278814.1 Latest version!	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa50696	XM_009296521.3 Latest version!	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa50696	XM_009296521.2	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNH1B ( NM_001278814.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001265743.1 Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), mRNA.

Price & Availability↑

CloneID	ODa36778
Clone ID Related Accession (Same CDS sequence)	NM_001278814.1
Accession Version	NM_001278814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	potassium voltage-gated channel, subfamily H (eag-related), member 1b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HQ703598.1. ##Evidence-Data-START## Transcript exon combination :: HQ703598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGACACCAA TTTTGTTTTG GGGAACGCCC AGATTGTGGA CTGGCCGATC 
GTCTACAGTA ATGACGGATT CTGCAAGCTG TCCGGTTACC ATCGTGCTGA AGTCATGCAG 
AAGAGCAGCA CATGCAGCTT CATGTACGGG GAACTTTCTG ACAAAGAAAC CAGCGAGAAA 
CTCCGCCAGA CCTTCGAGAA CTACGAGATG AACTCCTTTG AGTTACTGAT GTACAAGAAG 
AACCGGATGC CGGTGTGGTT CTTCGTCAAA ATCGCTCCCA TCCGAAACGA ACAGGACAAA 
GTTGTGCTTT TCCTCTGCAC CTTCAGCGAC ATCACTGCCT TCAAGCAGCC AATTGAAGAC 
GACTCCTCTA AAGGCTGGGG GAAGTTTGCT CGCCTGACAC GGGCGCTGAC CAGCAGTCGT 
GGTGTTTTAC AGCAGCTGGC GCCGACTGTC CAACGGGGAG AAAACGTGCT TAAACACTCA 
CGTCTGGCTG AGGTGTTGCA GCTGGGATCA GACATTCTCC CGCAGTACAA GCAAGAGGCC 
CCCAAAACGC CTCCACACAT CATCCTGCAC TACTGCACCT TCAAGACCAC CTGGGACTGG 
GTGATCCTCA TCCTCACCTT CTACACCGCC ATCATGGTGC CCTACAACGT GTCCTTTAAG 
ACCAAGCAGA ACAACGTCAC CTGGCTGGTG GTGGACAGCA TTGTGGATGT GATTTTCCTG 
GTGGACATTG TGCTCAACTT CCACACCACC TTTGTTGGGC CGGCAGGAGA GGTGATCTCA 
GACCCCAAAC TGATCCGTAT GAACTACGTC AAGACCTGGT TCGTGATCGA CCTGCTGTCC 
TGCCTGCCGT ACGACGTCAT TAACGCCTTC GAGAATGTTG ACGAGGGCAT CAGCAGTCTG 
TTCAGCTCTC TGAAGGTGGT CCGGTTGCTG CGTCTCGGCC GTGTGGCCCG TAAACTGGAC 
CACTACATTG AATACGGCGC AGCAGTGCTG GTTTTACTGG TGTGTGTGTT TGGTCTGGCC 
GCTCACTGGC TGGCCTGCAT CTGGTACAGC ATCGGAGACT ATGAGGTTAT CGATCAAGAC 
GCCAACATGG TGCGCACTGA CAGCTGGCTG TATCTGCTGG GCGAAGCTGT GGGTCAGCCC 
TATCACTTCA ACGCCAGCGG CTCAGGGAAG TGGGAAGGGG GTCCCAGCAA GGATTCGGTC 
TACATCACGT CTCTGTATTT CACCATGACC AGCCTGACCA GCATTGGATT TGGGAACATC 
GCTCCCACCA CCGACGGGGA GAAGATCTTC GCCGTGGCCA TGATGATGAT CGGATCTCTC 
CTTTATGCCA CCATCTTTGG TAACGTAACC AACATCTTCC AGCAAATGTA CGCCAACACC 
AACCGCTACC ACGAGATGTT AAACAGCGTG AGAGATTTCC TGAAGCTCTA CCAGGTTCCC 
AAAGGCCTCA GCGAGCGGGT CATGGATTAT ATCGTCTCCA CGTGGTCCAT GTCCAGAGGA 
ATCGACACTG ATAAGGTTTT GCAAATCTGC CCCAAAGACA TGCGAGCCGA CATCTGCGTT 
CATCTCAACA GGAAGGTGTT CAAAGAGCAC CCTGCGTTTC GCCTGGCCAG CGACGGCTGT 
TTAAGAGCTC TCGCTATGGA GTTTCAGACC ATCCACAGCG CGCCGGGAGA CCTCATATAC 
CACGCGGGCG AGAGTGTGGA CAGCCTCTGC TTTGTGGTGT CGGGGTCTTT GGAGGTCATC 
CAGGATGATG AAGTTGTTGC AATTTTGGGA AAGGGCGATG TGTTCGGCGA TGTGTTCTGG 
AAGGAGGTGA ATTTGGCTCA ATCCTGTGCA AACGTGCGAG CGCTGACCTA CTGCGACCTC 
CACATCATCA AGCGGGACGC TTTACAGAAG GTGCTGGAGT TTTACACCGC CTTCGCCGGA 
CACTTCTCAC GCAATCTGGT GCTCACCTAC AACCTGAGGA AAAGGATAGT CTTCCGCAAG 
ATCAGTGACG TGAAACGAGA GGAGGAGGAG ATGCTGCGGA GGAAGAACGA AGCTCCTCTG 
AATCTCCCTC CAGATCACCC GGTGCGGCGT CTTTTCCAGC GCTTTCGCCA GCAGAGAGAG 
GCTCGTCTGG CAGCGGAGCG GGCCAATCAG GTAGAGCAGG ACGTGGAGAA AGGTAAATCA 
GAGCAAACCG TGGTGAAAGA AATCACCTCA CCCACCAGCA TCATCACCGT AACAGAGAGT 
CCAGCAACAT TGAATACCTT ATCCCCGACC ACACCATCCA CCAAAACATT ATGCAGAGCA 
AAACTGAACA CTCCCCTAGC CCCGAACACA GCACAAAAAT CAGCAGAGGC TCCCAAAAGT 
AAAGGCTGGA GTCGGTTTAA GGAAGCCACG GCCAAATCTG ACCTCTCCTT AAGTTCTGTG 
TCCAAAGCCG AGTCCATGGA GATGCTGCCG GACAGGACTA AATCTCAAGA TGAGTTATTG 
TCCCTGAAGA AGACAGACTC CTGCGATAGT GGCATCACCA AGAGTGACCC GCGCCTAGAC 
AATATGGGCA GTGCCGGGAC CCCTCAGGAC CGGAGTCCGG TTCAGCCTGA AGACAAGCGC 
CTGTTTTACA CTATCCCGGA GCACAGTCTG CAGGCCTGCT TCTCGGAGCT AAGGCAAGAT 
CTGCAGGGGG ATATCCACTC GCTGGGCTGC AGGATGAGTG CACTTGAAGG ACAGCTGGCC 
GAGATGCTTA GACTGCTCGC AGAGAGAAAC TCCTCAGGAC CTGCAGAGCT GCACCACATT 
TCAAGACCAA ACTCGCCTGA TTCAGAAAAG GATGACAATA TATCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265743.1
CDS	1..2871
Translation	MAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFMYGELSDKETSEKLRQTFENYEMNSFELLMYKKNRMPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPIEDDSSKGWGKFARLTRALTSSRGVLQQLAPTVQRGENVLKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNVTWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEVISDPKLIRMNYVKTWFVIDLLSCLPYDVINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWLACIWYSIGDYEVIDQDANMVRTDSWLYLLGEAVGQPYHFNASGSGKWEGGPSKDSVYITSLYFTMTSLTSIGFGNIAPTTDGEKIFAVAMMMIGSLLYATIFGNVTNIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTDKVLQICPKDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTIHSAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEVNLAQSCANVRALTYCDLHIIKRDALQKVLEFYTAFAGHFSRNLVLTYNLRKRIVFRKISDVKREEEEMLRRKNEAPLNLPPDHPVRRLFQRFRQQREARLAAERANQVEQDVEKGKSEQTVVKEITSPTSIITVTESPATLNTLSPTTPSTKTLCRAKLNTPLAPNTAQKSAEAPKSKGWSRFKEATAKSDLSLSSVSKAESMEMLPDRTKSQDELLSLKKTDSCDSGITKSDPRLDNMGSAGTPQDRSPVQPEDKRLFYTIPEHSLQACFSELRQDLQGDIHSLGCRMSALEGQLAEMLRLLAERNSSGPAELHHISRPNSPDSEKDDNISS

Target ORF information:

RefSeq Version	NM_001278814.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278814.1

ORF Insert Sequence:

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGACACCAA TTTTGTTTTG GGGAACGCCC AGATTGTGGA CTGGCCGATC 
GTCTACAGTA ATGACGGATT CTGCAAGCTG TCCGGTTACC ATCGTGCTGA AGTCATGCAG 
AAGAGCAGCA CATGCAGCTT CATGTACGGG GAACTTTCTG ACAAAGAAAC CAGCGAGAAA 
CTCCGCCAGA CCTTCGAGAA CTACGAGATG AACTCCTTTG AGTTACTGAT GTACAAGAAG 
AACCGGATGC CGGTGTGGTT CTTCGTCAAA ATCGCTCCCA TCCGAAACGA ACAGGACAAA 
GTTGTGCTTT TCCTCTGCAC CTTCAGCGAC ATCACTGCCT TCAAGCAGCC AATTGAAGAC 
GACTCCTCTA AAGGCTGGGG GAAGTTTGCT CGCCTGACAC GGGCGCTGAC CAGCAGTCGT 
GGTGTTTTAC AGCAGCTGGC GCCGACTGTC CAACGGGGAG AAAACGTGCT TAAACACTCA 
CGTCTGGCTG AGGTGTTGCA GCTGGGATCA GACATTCTCC CGCAGTACAA GCAAGAGGCC 
CCCAAAACGC CTCCACACAT CATCCTGCAC TACTGCACCT TCAAGACCAC CTGGGACTGG 
GTGATCCTCA TCCTCACCTT CTACACCGCC ATCATGGTGC CCTACAACGT GTCCTTTAAG 
ACCAAGCAGA ACAACGTCAC CTGGCTGGTG GTGGACAGCA TTGTGGATGT GATTTTCCTG 
GTGGACATTG TGCTCAACTT CCACACCACC TTTGTTGGGC CGGCAGGAGA GGTGATCTCA 
GACCCCAAAC TGATCCGTAT GAACTACGTC AAGACCTGGT TCGTGATCGA CCTGCTGTCC 
TGCCTGCCGT ACGACGTCAT TAACGCCTTC GAGAATGTTG ACGAGGGCAT CAGCAGTCTG 
TTCAGCTCTC TGAAGGTGGT CCGGTTGCTG CGTCTCGGCC GTGTGGCCCG TAAACTGGAC 
CACTACATTG AATACGGCGC AGCAGTGCTG GTTTTACTGG TGTGTGTGTT TGGTCTGGCC 
GCTCACTGGC TGGCCTGCAT CTGGTACAGC ATCGGAGACT ATGAGGTTAT CGATCAAGAC 
GCCAACATGG TGCGCACTGA CAGCTGGCTG TATCTGCTGG GCGAAGCTGT GGGTCAGCCC 
TATCACTTCA ACGCCAGCGG CTCAGGGAAG TGGGAAGGGG GTCCCAGCAA GGATTCGGTC 
TACATCACGT CTCTGTATTT CACCATGACC AGCCTGACCA GCATTGGATT TGGGAACATC 
GCTCCCACCA CCGACGGGGA GAAGATCTTC GCCGTGGCCA TGATGATGAT CGGATCTCTC 
CTTTATGCCA CCATCTTTGG TAACGTAACC AACATCTTCC AGCAAATGTA CGCCAACACC 
AACCGCTACC ACGAGATGTT AAACAGCGTG AGAGATTTCC TGAAGCTCTA CCAGGTTCCC 
AAAGGCCTCA GCGAGCGGGT CATGGATTAT ATCGTCTCCA CGTGGTCCAT GTCCAGAGGA 
ATCGACACTG ATAAGGTTTT GCAAATCTGC CCCAAAGACA TGCGAGCCGA CATCTGCGTT 
CATCTCAACA GGAAGGTGTT CAAAGAGCAC CCTGCGTTTC GCCTGGCCAG CGACGGCTGT 
TTAAGAGCTC TCGCTATGGA GTTTCAGACC ATCCACAGCG CGCCGGGAGA CCTCATATAC 
CACGCGGGCG AGAGTGTGGA CAGCCTCTGC TTTGTGGTGT CGGGGTCTTT GGAGGTCATC 
CAGGATGATG AAGTTGTTGC AATTTTGGGA AAGGGCGATG TGTTCGGCGA TGTGTTCTGG 
AAGGAGGTGA ATTTGGCTCA ATCCTGTGCA AACGTGCGAG CGCTGACCTA CTGCGACCTC 
CACATCATCA AGCGGGACGC TTTACAGAAG GTGCTGGAGT TTTACACCGC CTTCGCCGGA 
CACTTCTCAC GCAATCTGGT GCTCACCTAC AACCTGAGGA AAAGGATAGT CTTCCGCAAG 
ATCAGTGACG TGAAACGAGA GGAGGAGGAG ATGCTGCGGA GGAAGAACGA AGCTCCTCTG 
AATCTCCCTC CAGATCACCC GGTGCGGCGT CTTTTCCAGC GCTTTCGCCA GCAGAGAGAG 
GCTCGTCTGG CAGCGGAGCG GGCCAATCAG GTAGAGCAGG ACGTGGAGAA AGGTAAATCA 
GAGCAAACCG TGGTGAAAGA AATCACCTCA CCCACCAGCA TCATCACCGT AACAGAGAGT 
CCAGCAACAT TGAATACCTT ATCCCCGACC ACACCATCCA CCAAAACATT ATGCAGAGCA 
AAACTGAACA CTCCCCTAGC CCCGAACACA GCACAAAAAT CAGCAGAGGC TCCCAAAAGT 
AAAGGCTGGA GTCGGTTTAA GGAAGCCACG GCCAAATCTG ACCTCTCCTT AAGTTCTGTG 
TCCAAAGCCG AGTCCATGGA GATGCTGCCG GACAGGACTA AATCTCAAGA TGAGTTATTG 
TCCCTGAAGA AGACAGACTC CTGCGATAGT GGCATCACCA AGAGTGACCC GCGCCTAGAC 
AATATGGGCA GTGCCGGGAC CCCTCAGGAC CGGAGTCCGG TTCAGCCTGA AGACAAGCGC 
CTGTTTTACA CTATCCCGGA GCACAGTCTG CAGGCCTGCT TCTCGGAGCT AAGGCAAGAT 
CTGCAGGGGG ATATCCACTC GCTGGGCTGC AGGATGAGTG CACTTGAAGG ACAGCTGGCC 
GAGATGCTTA GACTGCTCGC AGAGAGAAAC TCCTCAGGAC CTGCAGAGCT GCACCACATT 
TCAAGACCAA ACTCGCCTGA TTCAGAAAAG GATGACAATA TATCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNH1B ( XM_009296521.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294796.1 Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa50696
Clone ID Related Accession (Same CDS sequence)	XM_009296521.3 , XM_009296521.2
Accession Version	XM_009296521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium voltage-gated channel, subfamily H (eag-related), member 1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGTTTGGAC ATTTTCTCAT AGACAGACAG ACACCAATTT TGTTTTGGGG 
AACGCCCAGA TTGTGGACTG GCCGATCGTC TACAGTAATG ACGGATTCTG CAAGCTGTCC 
GGTTACCATC GTGCTGAAGT CATGCAGAAG AGCAGCACAT GCAGCTTCAT GTACGGGGAA 
CTTTCTGACA AAGAAACCAG TGAGAAACTC CGCCAGACCT TCGAGAACTA CGAGATGAAC 
TCCTTTGAGT TACTGATGTA CAAGAAGAAC CGGATGCCAG TGTGGTTCTT CGTCAAAATC 
GCTCCCATCC GAAACGAACA GGACAAAGTT GTGCTTTTCC TCTGCACCTT CAGCGACATC 
ACTGCCTTCA AGCAGCCAAT TGAAGACGAC TCCTCTAAAG GCTGGGGGAA GTTTGCTCGC 
CTGACACGGG CGCTGACCAG CAGTCGTGGT GTTTTACAGC AGCTGGCGCC GACTGTCCAA 
CGGGGAGAAA ACGTGCTTAA ACACTCACGT CTGGCTGAGG TGTTGCAGCT GGGATCAGAC 
ATTCTCCCGC AGTACAAGCA AGAGGCTCCC AAAACGCCTC CGCACATCAT CCTGCACTAC 
TGCACCTTCA AGACCACCTG GGACTGGGTG ATCCTCATCC TCACCTTCTA CACCGCCATC 
ATGGTGCCCT ACAACGTGTC CTTTAAGACC AAGCAGAACA ACGTCACCTG GCTGGTGGTG 
GACAGCATTG TGGATGTGAT TTTCCTGGTG GACATTGTGC TCAACTTCCA CACCACCTTT 
GTTGGGCCGG CGGGAGAGGT GATCTCAGAC CCCAAACTGA TCCGTATGAA CTACGTCAAG 
ACCTGGTTCG TGATCGACCT GCTGTCCTGC CTGCCGTACG ACGTCATTAA CGCCTTCGAG 
AATGTTGACG AGGGCATCAG CAGTCTGTTC AGCTCTCTGA AGGTGGTCCG GTTGCTGCGT 
CTCGGCCGTG TGGCCCGTAA ACTGGACCAC TACATTGAAT ACGGCGCAGC AGTGCTGGTT 
TTACTGGTGT GTGTGTTTGG TCTGGCCGCT CACTGGCTGG CCTGCATCTG GTACAGCATC 
GGAGACTATG AGGTTATCGA TCAAGACGCC AACATGGTGC GCACTGACAG CTGGCTGTAT 
CTGCTGGGCG AAGCTGTGGG TCAGCCCTAT CACTTCAACG CCAGCGGCTC AGGGAAGTGG 
GAAGGGGGTC CCAGCAAGGA TTCGGTCTAC ATCACGTCTC TGTATTTCAC CATGACCAGC 
CTGACCAGCA TTGGATTTGG GAACATCGCT CCCACCACCG ACGGGGAGAA GATCTTCGCC 
GTGGCCATGA TGATGATCGG ATCTCTCCTT TATGCCACCA TCTTTGGTAA CGTAACCAAC 
ATCTTCCAGC AAATGTACGC CAACACCAAC CGCTACCACG AGATGTTAAA CAGCGTGAGA 
GATTTCCTGA AGCTCTACCA GGTTCCCAAA GGCCTCAGCG AGCGGGTCAT GGATTATATC 
GTCTCCACGT GGTCCATGTC CAGAGGAATC GACACTGATA AGGTTTTGCA AATCTGCCCC 
AAAGACATGC GAGCCGACAT CTGCGTTCAT CTCAACAGGA AGGTGTTCAA AGAGCACCCT 
GCGTTTCGCC TGGCCAGCGA CGGCTGTTTA AGAGCTCTCG CTATGGAGTT TCAGACCATC 
CACAGCGCGC CGGGAGACCT CATATACCAC GCGGGCGAGA GTGTGGACAG CCTCTGCTTT 
GTGGTGTCGG GGTCTTTGGA GGTCATCCAG GATGATGAAG TTGTTGCAAT TTTGGGAAAG 
GGCGATGTGT TCGGCGATGT GTTCTGGAAG GAGGTGAATT TGGCTCAATC CTGTGCAAAC 
GTGCGAGCGC TGACCTACTG CGACCTCCAC ATCATCAAGC GGGACGCTTT ACAGAAGGTG 
CTGGAGTTTT ACACCGCCTT CGCCGGACAC TTCTCACGCA ATCTGGTGCT CACCTACAAC 
CTGAGGAAAA GGATAGTCTT CCGCAAGATC AGTGACGTGA AACGAGAGGA AGAGGAGATG 
CTGCGGAGGA AGAACGAAGC TCCTCTGAAT CTCCCTCCAG ATCACCCGGT GCGGCGTCTT 
TTCCAGCGCT TTCGGCAGCA GAGAGAGGCT CGTCTGGCAG CGGAGCGGGC CAATCAGGTA 
GAGCAGGACG TGGAGAAAGG TAAATCAGAG CAAACCGTGG TGAAAGAAAT CACCTCACCC 
ACCAGCATCA TCACCGTAAC AGAGAGTCCA GCCACATTGA ATACCTTATC CCCGACCACA 
CCATCCACCA AAACATTATG CAGAGCAAAA CTGAACACTC CCCTAGCCCC GAACACAGCA 
CAAAAATCAG CAGAAGCTCC CAAAAGTAAA GGCTGGAGTC GGTTTAAGGA AGCCACGGCC 
AAATCTGACC TCTCCTTAAG TTCTGTGTCC AAAGCCGAGT CCATGGAGAT GCTGCCGGAC 
AGGACTAAAT CTCAAGATGA GTTATTGTCC CTGAAGAAGA CAGACTCCTG CGATAGTGGC 
ATCACCAAGA GTGACCCGCG CCTAGACAAT ATGGGCAGTG CCGAGACCCC TCAGGACCGG 
AGCCCGGTTC AGCCTGAAGA AAAGCGCCTG TTTTACCCTA TCCCGGAGCA CAGTCTGCAG 
GCCTGCTTCT CGGAGCTAAG GCAAGATCTG CAGGGGGATA TCCACTCGCT GGGCTGCAGG 
ATGAGTGCAC TTGAAGGACA GCTGGCCGAG ATGCTAAGAC TGCTCGCAGA GAGAAACTCC 
TCAGGACCTG CAGAGCTGCA CCACATTTCA AGACCAAACT CGCCTGATTC AGAAAAGGAT 
GACAATATAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294796.1
CDS	103..3000
Translation	MAGGRRGLVAPQNTFLENIVRRSNVWTFSHRQTDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFMYGELSDKETSEKLRQTFENYEMNSFELLMYKKNRMPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPIEDDSSKGWGKFARLTRALTSSRGVLQQLAPTVQRGENVLKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNVTWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEVISDPKLIRMNYVKTWFVIDLLSCLPYDVINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWLACIWYSIGDYEVIDQDANMVRTDSWLYLLGEAVGQPYHFNASGSGKWEGGPSKDSVYITSLYFTMTSLTSIGFGNIAPTTDGEKIFAVAMMMIGSLLYATIFGNVTNIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTDKVLQICPKDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTIHSAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEVNLAQSCANVRALTYCDLHIIKRDALQKVLEFYTAFAGHFSRNLVLTYNLRKRIVFRKISDVKREEEEMLRRKNEAPLNLPPDHPVRRLFQRFRQQREARLAAERANQVEQDVEKGKSEQTVVKEITSPTSIITVTESPATLNTLSPTTPSTKTLCRAKLNTPLAPNTAQKSAEAPKSKGWSRFKEATAKSDLSLSSVSKAESMEMLPDRTKSQDELLSLKKTDSCDSGITKSDPRLDNMGSAETPQDRSPVQPEEKRLFYPIPEHSLQACFSELRQDLQGDIHSLGCRMSALEGQLAEMLRLLAERNSSGPAELHHISRPNSPDSEKDDNISS

Target ORF information:

RefSeq Version	XM_009296521.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296521.3

ORF Insert Sequence:

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGTTTGGAC ATTTTCTCAT AGACAGACAG ACACCAATTT TGTTTTGGGG 
AACGCCCAGA TTGTGGACTG GCCGATCGTC TACAGTAATG ACGGATTCTG CAAGCTGTCC 
GGTTACCATC GTGCTGAAGT CATGCAGAAG AGCAGCACAT GCAGCTTCAT GTACGGGGAA 
CTTTCTGACA AAGAAACCAG TGAGAAACTC CGCCAGACCT TCGAGAACTA CGAGATGAAC 
TCCTTTGAGT TACTGATGTA CAAGAAGAAC CGGATGCCAG TGTGGTTCTT CGTCAAAATC 
GCTCCCATCC GAAACGAACA GGACAAAGTT GTGCTTTTCC TCTGCACCTT CAGCGACATC 
ACTGCCTTCA AGCAGCCAAT TGAAGACGAC TCCTCTAAAG GCTGGGGGAA GTTTGCTCGC 
CTGACACGGG CGCTGACCAG CAGTCGTGGT GTTTTACAGC AGCTGGCGCC GACTGTCCAA 
CGGGGAGAAA ACGTGCTTAA ACACTCACGT CTGGCTGAGG TGTTGCAGCT GGGATCAGAC 
ATTCTCCCGC AGTACAAGCA AGAGGCTCCC AAAACGCCTC CGCACATCAT CCTGCACTAC 
TGCACCTTCA AGACCACCTG GGACTGGGTG ATCCTCATCC TCACCTTCTA CACCGCCATC 
ATGGTGCCCT ACAACGTGTC CTTTAAGACC AAGCAGAACA ACGTCACCTG GCTGGTGGTG 
GACAGCATTG TGGATGTGAT TTTCCTGGTG GACATTGTGC TCAACTTCCA CACCACCTTT 
GTTGGGCCGG CGGGAGAGGT GATCTCAGAC CCCAAACTGA TCCGTATGAA CTACGTCAAG 
ACCTGGTTCG TGATCGACCT GCTGTCCTGC CTGCCGTACG ACGTCATTAA CGCCTTCGAG 
AATGTTGACG AGGGCATCAG CAGTCTGTTC AGCTCTCTGA AGGTGGTCCG GTTGCTGCGT 
CTCGGCCGTG TGGCCCGTAA ACTGGACCAC TACATTGAAT ACGGCGCAGC AGTGCTGGTT 
TTACTGGTGT GTGTGTTTGG TCTGGCCGCT CACTGGCTGG CCTGCATCTG GTACAGCATC 
GGAGACTATG AGGTTATCGA TCAAGACGCC AACATGGTGC GCACTGACAG CTGGCTGTAT 
CTGCTGGGCG AAGCTGTGGG TCAGCCCTAT CACTTCAACG CCAGCGGCTC AGGGAAGTGG 
GAAGGGGGTC CCAGCAAGGA TTCGGTCTAC ATCACGTCTC TGTATTTCAC CATGACCAGC 
CTGACCAGCA TTGGATTTGG GAACATCGCT CCCACCACCG ACGGGGAGAA GATCTTCGCC 
GTGGCCATGA TGATGATCGG ATCTCTCCTT TATGCCACCA TCTTTGGTAA CGTAACCAAC 
ATCTTCCAGC AAATGTACGC CAACACCAAC CGCTACCACG AGATGTTAAA CAGCGTGAGA 
GATTTCCTGA AGCTCTACCA GGTTCCCAAA GGCCTCAGCG AGCGGGTCAT GGATTATATC 
GTCTCCACGT GGTCCATGTC CAGAGGAATC GACACTGATA AGGTTTTGCA AATCTGCCCC 
AAAGACATGC GAGCCGACAT CTGCGTTCAT CTCAACAGGA AGGTGTTCAA AGAGCACCCT 
GCGTTTCGCC TGGCCAGCGA CGGCTGTTTA AGAGCTCTCG CTATGGAGTT TCAGACCATC 
CACAGCGCGC CGGGAGACCT CATATACCAC GCGGGCGAGA GTGTGGACAG CCTCTGCTTT 
GTGGTGTCGG GGTCTTTGGA GGTCATCCAG GATGATGAAG TTGTTGCAAT TTTGGGAAAG 
GGCGATGTGT TCGGCGATGT GTTCTGGAAG GAGGTGAATT TGGCTCAATC CTGTGCAAAC 
GTGCGAGCGC TGACCTACTG CGACCTCCAC ATCATCAAGC GGGACGCTTT ACAGAAGGTG 
CTGGAGTTTT ACACCGCCTT CGCCGGACAC TTCTCACGCA ATCTGGTGCT CACCTACAAC 
CTGAGGAAAA GGATAGTCTT CCGCAAGATC AGTGACGTGA AACGAGAGGA AGAGGAGATG 
CTGCGGAGGA AGAACGAAGC TCCTCTGAAT CTCCCTCCAG ATCACCCGGT GCGGCGTCTT 
TTCCAGCGCT TTCGGCAGCA GAGAGAGGCT CGTCTGGCAG CGGAGCGGGC CAATCAGGTA 
GAGCAGGACG TGGAGAAAGG TAAATCAGAG CAAACCGTGG TGAAAGAAAT CACCTCACCC 
ACCAGCATCA TCACCGTAAC AGAGAGTCCA GCCACATTGA ATACCTTATC CCCGACCACA 
CCATCCACCA AAACATTATG CAGAGCAAAA CTGAACACTC CCCTAGCCCC GAACACAGCA 
CAAAAATCAG CAGAAGCTCC CAAAAGTAAA GGCTGGAGTC GGTTTAAGGA AGCCACGGCC 
AAATCTGACC TCTCCTTAAG TTCTGTGTCC AAAGCCGAGT CCATGGAGAT GCTGCCGGAC 
AGGACTAAAT CTCAAGATGA GTTATTGTCC CTGAAGAAGA CAGACTCCTG CGATAGTGGC 
ATCACCAAGA GTGACCCGCG CCTAGACAAT ATGGGCAGTG CCGAGACCCC TCAGGACCGG 
AGCCCGGTTC AGCCTGAAGA AAAGCGCCTG TTTTACCCTA TCCCGGAGCA CAGTCTGCAG 
GCCTGCTTCT CGGAGCTAAG GCAAGATCTG CAGGGGGATA TCCACTCGCT GGGCTGCAGG 
ATGAGTGCAC TTGAAGGACA GCTGGCCGAG ATGCTAAGAC TGCTCGCAGA GAGAAACTCC 
TCAGGACCTG CAGAGCTGCA CCACATTTCA AGACCAAACT CGCCTGATTC AGAAAAGGAT 
GACAATATAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNH1B ( XM_009296521.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294796.1 Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.

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CloneID	ODa50696
Clone ID Related Accession (Same CDS sequence)	XM_009296521.3 , XM_009296521.2
Accession Version	XM_009296521.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	potassium voltage-gated channel, subfamily H (eag-related), member 1b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGTTTGGAC ATTTTCTCAT AGACAGACAG ACACCAATTT TGTTTTGGGG 
AACGCCCAGA TTGTGGACTG GCCGATCGTC TACAGTAATG ACGGATTCTG CAAGCTGTCC 
GGTTACCATC GTGCTGAAGT CATGCAGAAG AGCAGCACAT GCAGCTTCAT GTACGGGGAA 
CTTTCTGACA AAGAAACCAG TGAGAAACTC CGCCAGACCT TCGAGAACTA CGAGATGAAC 
TCCTTTGAGT TACTGATGTA CAAGAAGAAC CGGATGCCAG TGTGGTTCTT CGTCAAAATC 
GCTCCCATCC GAAACGAACA GGACAAAGTT GTGCTTTTCC TCTGCACCTT CAGCGACATC 
ACTGCCTTCA AGCAGCCAAT TGAAGACGAC TCCTCTAAAG GCTGGGGGAA GTTTGCTCGC 
CTGACACGGG CGCTGACCAG CAGTCGTGGT GTTTTACAGC AGCTGGCGCC GACTGTCCAA 
CGGGGAGAAA ACGTGCTTAA ACACTCACGT CTGGCTGAGG TGTTGCAGCT GGGATCAGAC 
ATTCTCCCGC AGTACAAGCA AGAGGCTCCC AAAACGCCTC CGCACATCAT CCTGCACTAC 
TGCACCTTCA AGACCACCTG GGACTGGGTG ATCCTCATCC TCACCTTCTA CACCGCCATC 
ATGGTGCCCT ACAACGTGTC CTTTAAGACC AAGCAGAACA ACGTCACCTG GCTGGTGGTG 
GACAGCATTG TGGATGTGAT TTTCCTGGTG GACATTGTGC TCAACTTCCA CACCACCTTT 
GTTGGGCCGG CGGGAGAGGT GATCTCAGAC CCCAAACTGA TCCGTATGAA CTACGTCAAG 
ACCTGGTTCG TGATCGACCT GCTGTCCTGC CTGCCGTACG ACGTCATTAA CGCCTTCGAG 
AATGTTGACG AGGGCATCAG CAGTCTGTTC AGCTCTCTGA AGGTGGTCCG GTTGCTGCGT 
CTCGGCCGTG TGGCCCGTAA ACTGGACCAC TACATTGAAT ACGGCGCAGC AGTGCTGGTT 
TTACTGGTGT GTGTGTTTGG TCTGGCCGCT CACTGGCTGG CCTGCATCTG GTACAGCATC 
GGAGACTATG AGGTTATCGA TCAAGACGCC AACATGGTGC GCACTGACAG CTGGCTGTAT 
CTGCTGGGCG AAGCTGTGGG TCAGCCCTAT CACTTCAACG CCAGCGGCTC AGGGAAGTGG 
GAAGGGGGTC CCAGCAAGGA TTCGGTCTAC ATCACGTCTC TGTATTTCAC CATGACCAGC 
CTGACCAGCA TTGGATTTGG GAACATCGCT CCCACCACCG ACGGGGAGAA GATCTTCGCC 
GTGGCCATGA TGATGATCGG ATCTCTCCTT TATGCCACCA TCTTTGGTAA CGTAACCAAC 
ATCTTCCAGC AAATGTACGC CAACACCAAC CGCTACCACG AGATGTTAAA CAGCGTGAGA 
GATTTCCTGA AGCTCTACCA GGTTCCCAAA GGCCTCAGCG AGCGGGTCAT GGATTATATC 
GTCTCCACGT GGTCCATGTC CAGAGGAATC GACACTGATA AGGTTTTGCA AATCTGCCCC 
AAAGACATGC GAGCCGACAT CTGCGTTCAT CTCAACAGGA AGGTGTTCAA AGAGCACCCT 
GCGTTTCGCC TGGCCAGCGA CGGCTGTTTA AGAGCTCTCG CTATGGAGTT TCAGACCATC 
CACAGCGCGC CGGGAGACCT CATATACCAC GCGGGCGAGA GTGTGGACAG CCTCTGCTTT 
GTGGTGTCGG GGTCTTTGGA GGTCATCCAG GATGATGAAG TTGTTGCAAT TTTGGGAAAG 
GGCGATGTGT TCGGCGATGT GTTCTGGAAG GAGGTGAATT TGGCTCAATC CTGTGCAAAC 
GTGCGAGCGC TGACCTACTG CGACCTCCAC ATCATCAAGC GGGACGCTTT ACAGAAGGTG 
CTGGAGTTTT ACACCGCCTT CGCCGGACAC TTCTCACGCA ATCTGGTGCT CACCTACAAC 
CTGAGGAAAA GGATAGTCTT CCGCAAGATC AGTGACGTGA AACGAGAGGA AGAGGAGATG 
CTGCGGAGGA AGAACGAAGC TCCTCTGAAT CTCCCTCCAG ATCACCCGGT GCGGCGTCTT 
TTCCAGCGCT TTCGGCAGCA GAGAGAGGCT CGTCTGGCAG CGGAGCGGGC CAATCAGGTA 
GAGCAGGACG TGGAGAAAGG TAAATCAGAG CAAACCGTGG TGAAAGAAAT CACCTCACCC 
ACCAGCATCA TCACCGTAAC AGAGAGTCCA GCCACATTGA ATACCTTATC CCCGACCACA 
CCATCCACCA AAACATTATG CAGAGCAAAA CTGAACACTC CCCTAGCCCC GAACACAGCA 
CAAAAATCAG CAGAAGCTCC CAAAAGTAAA GGCTGGAGTC GGTTTAAGGA AGCCACGGCC 
AAATCTGACC TCTCCTTAAG TTCTGTGTCC AAAGCCGAGT CCATGGAGAT GCTGCCGGAC 
AGGACTAAAT CTCAAGATGA GTTATTGTCC CTGAAGAAGA CAGACTCCTG CGATAGTGGC 
ATCACCAAGA GTGACCCGCG CCTAGACAAT ATGGGCAGTG CCGAGACCCC TCAGGACCGG 
AGCCCGGTTC AGCCTGAAGA AAAGCGCCTG TTTTACCCTA TCCCGGAGCA CAGTCTGCAG 
GCCTGCTTCT CGGAGCTAAG GCAAGATCTG CAGGGGGATA TCCACTCGCT GGGCTGCAGG 
ATGAGTGCAC TTGAAGGACA GCTGGCCGAG ATGCTAAGAC TGCTCGCAGA GAGAAACTCC 
TCAGGACCTG CAGAGCTGCA CCACATTTCA AGACCAAACT CGCCTGATTC AGAAAAGGAT 
GACAATATAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294796.1
CDS	103..3000
Translation	MAGGRRGLVAPQNTFLENIVRRSNVWTFSHRQTDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFMYGELSDKETSEKLRQTFENYEMNSFELLMYKKNRMPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPIEDDSSKGWGKFARLTRALTSSRGVLQQLAPTVQRGENVLKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNVTWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEVISDPKLIRMNYVKTWFVIDLLSCLPYDVINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWLACIWYSIGDYEVIDQDANMVRTDSWLYLLGEAVGQPYHFNASGSGKWEGGPSKDSVYITSLYFTMTSLTSIGFGNIAPTTDGEKIFAVAMMMIGSLLYATIFGNVTNIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTDKVLQICPKDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTIHSAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEVNLAQSCANVRALTYCDLHIIKRDALQKVLEFYTAFAGHFSRNLVLTYNLRKRIVFRKISDVKREEEEMLRRKNEAPLNLPPDHPVRRLFQRFRQQREARLAAERANQVEQDVEKGKSEQTVVKEITSPTSIITVTESPATLNTLSPTTPSTKTLCRAKLNTPLAPNTAQKSAEAPKSKGWSRFKEATAKSDLSLSSVSKAESMEMLPDRTKSQDELLSLKKTDSCDSGITKSDPRLDNMGSAETPQDRSPVQPEEKRLFYPIPEHSLQACFSELRQDLQGDIHSLGCRMSALEGQLAEMLRLLAERNSSGPAELHHISRPNSPDSEKDDNISS

Target ORF information:

RefSeq Version	XM_009296521.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio potassium voltage-gated channel, subfamily H (eag-related), member 1b (kcnh1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296521.2

ORF Insert Sequence:

ATGGCGGGGG GACGCAGAGG ACTGGTGGCC CCTCAGAACA CATTCCTGGA GAATATAGTG 
CGGCGCTCCA ACGTTTGGAC ATTTTCTCAT AGACAGACAG ACACCAATTT TGTTTTGGGG 
AACGCCCAGA TTGTGGACTG GCCGATCGTC TACAGTAATG ACGGATTCTG CAAGCTGTCC 
GGTTACCATC GTGCTGAAGT CATGCAGAAG AGCAGCACAT GCAGCTTCAT GTACGGGGAA 
CTTTCTGACA AAGAAACCAG TGAGAAACTC CGCCAGACCT TCGAGAACTA CGAGATGAAC 
TCCTTTGAGT TACTGATGTA CAAGAAGAAC CGGATGCCAG TGTGGTTCTT CGTCAAAATC 
GCTCCCATCC GAAACGAACA GGACAAAGTT GTGCTTTTCC TCTGCACCTT CAGCGACATC 
ACTGCCTTCA AGCAGCCAAT TGAAGACGAC TCCTCTAAAG GCTGGGGGAA GTTTGCTCGC 
CTGACACGGG CGCTGACCAG CAGTCGTGGT GTTTTACAGC AGCTGGCGCC GACTGTCCAA 
CGGGGAGAAA ACGTGCTTAA ACACTCACGT CTGGCTGAGG TGTTGCAGCT GGGATCAGAC 
ATTCTCCCGC AGTACAAGCA AGAGGCTCCC AAAACGCCTC CGCACATCAT CCTGCACTAC 
TGCACCTTCA AGACCACCTG GGACTGGGTG ATCCTCATCC TCACCTTCTA CACCGCCATC 
ATGGTGCCCT ACAACGTGTC CTTTAAGACC AAGCAGAACA ACGTCACCTG GCTGGTGGTG 
GACAGCATTG TGGATGTGAT TTTCCTGGTG GACATTGTGC TCAACTTCCA CACCACCTTT 
GTTGGGCCGG CGGGAGAGGT GATCTCAGAC CCCAAACTGA TCCGTATGAA CTACGTCAAG 
ACCTGGTTCG TGATCGACCT GCTGTCCTGC CTGCCGTACG ACGTCATTAA CGCCTTCGAG 
AATGTTGACG AGGGCATCAG CAGTCTGTTC AGCTCTCTGA AGGTGGTCCG GTTGCTGCGT 
CTCGGCCGTG TGGCCCGTAA ACTGGACCAC TACATTGAAT ACGGCGCAGC AGTGCTGGTT 
TTACTGGTGT GTGTGTTTGG TCTGGCCGCT CACTGGCTGG CCTGCATCTG GTACAGCATC 
GGAGACTATG AGGTTATCGA TCAAGACGCC AACATGGTGC GCACTGACAG CTGGCTGTAT 
CTGCTGGGCG AAGCTGTGGG TCAGCCCTAT CACTTCAACG CCAGCGGCTC AGGGAAGTGG 
GAAGGGGGTC CCAGCAAGGA TTCGGTCTAC ATCACGTCTC TGTATTTCAC CATGACCAGC 
CTGACCAGCA TTGGATTTGG GAACATCGCT CCCACCACCG ACGGGGAGAA GATCTTCGCC 
GTGGCCATGA TGATGATCGG ATCTCTCCTT TATGCCACCA TCTTTGGTAA CGTAACCAAC 
ATCTTCCAGC AAATGTACGC CAACACCAAC CGCTACCACG AGATGTTAAA CAGCGTGAGA 
GATTTCCTGA AGCTCTACCA GGTTCCCAAA GGCCTCAGCG AGCGGGTCAT GGATTATATC 
GTCTCCACGT GGTCCATGTC CAGAGGAATC GACACTGATA AGGTTTTGCA AATCTGCCCC 
AAAGACATGC GAGCCGACAT CTGCGTTCAT CTCAACAGGA AGGTGTTCAA AGAGCACCCT 
GCGTTTCGCC TGGCCAGCGA CGGCTGTTTA AGAGCTCTCG CTATGGAGTT TCAGACCATC 
CACAGCGCGC CGGGAGACCT CATATACCAC GCGGGCGAGA GTGTGGACAG CCTCTGCTTT 
GTGGTGTCGG GGTCTTTGGA GGTCATCCAG GATGATGAAG TTGTTGCAAT TTTGGGAAAG 
GGCGATGTGT TCGGCGATGT GTTCTGGAAG GAGGTGAATT TGGCTCAATC CTGTGCAAAC 
GTGCGAGCGC TGACCTACTG CGACCTCCAC ATCATCAAGC GGGACGCTTT ACAGAAGGTG 
CTGGAGTTTT ACACCGCCTT CGCCGGACAC TTCTCACGCA ATCTGGTGCT CACCTACAAC 
CTGAGGAAAA GGATAGTCTT CCGCAAGATC AGTGACGTGA AACGAGAGGA AGAGGAGATG 
CTGCGGAGGA AGAACGAAGC TCCTCTGAAT CTCCCTCCAG ATCACCCGGT GCGGCGTCTT 
TTCCAGCGCT TTCGGCAGCA GAGAGAGGCT CGTCTGGCAG CGGAGCGGGC CAATCAGGTA 
GAGCAGGACG TGGAGAAAGG TAAATCAGAG CAAACCGTGG TGAAAGAAAT CACCTCACCC 
ACCAGCATCA TCACCGTAAC AGAGAGTCCA GCCACATTGA ATACCTTATC CCCGACCACA 
CCATCCACCA AAACATTATG CAGAGCAAAA CTGAACACTC CCCTAGCCCC GAACACAGCA 
CAAAAATCAG CAGAAGCTCC CAAAAGTAAA GGCTGGAGTC GGTTTAAGGA AGCCACGGCC 
AAATCTGACC TCTCCTTAAG TTCTGTGTCC AAAGCCGAGT CCATGGAGAT GCTGCCGGAC 
AGGACTAAAT CTCAAGATGA GTTATTGTCC CTGAAGAAGA CAGACTCCTG CGATAGTGGC 
ATCACCAAGA GTGACCCGCG CCTAGACAAT ATGGGCAGTG CCGAGACCCC TCAGGACCGG 
AGCCCGGTTC AGCCTGAAGA AAAGCGCCTG TTTTACCCTA TCCCGGAGCA CAGTCTGCAG 
GCCTGCTTCT CGGAGCTAAG GCAAGATCTG CAGGGGGATA TCCACTCGCT GGGCTGCAGG 
ATGAGTGCAC TTGAAGGACA GCTGGCCGAG ATGCTAAGAC TGCTCGCAGA GAGAAACTCC 
TCAGGACCTG CAGAGCTGCA CCACATTTCA AGACCAAACT CGCCTGATTC AGAAAAGGAT 
GACAATATAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Kcnh1 voltage-gated potassium channels are essential for early zebrafish development.
The Journal of biological chemistry287(42)35565-75(2012 Oct)
Stengel R,Rivera-Milla E,Sahoo N,Ebert C,Bollig F,Heinemann SH,Schönherr R,Englert C

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KCNH1B cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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