mcm3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	mcm3
Entrez Gene ID	192323
Full Name	minichromosome maintenance complex component 3
Synonyms	MCM3z,cb32,chunp6867,wu:fa26g03
General protein information	Preferred Names minichromosome maintenance complex component 3 Names DNA replication licensing factor MCM3 MCM3 minichromosome maintenance deficient 3 zygotic Mcm3 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_212567.2 NP_997732.2 DNA replication licensing factor MCM3
Pathways from BioSystems

mRNA	Protein	Name
NM_212567.2	NP_997732.2	DNA replication licensing factor MCM3

Homologies

Mus musculus (house mouse)	Mcm3	NP_032589.1
Eremothecium gossypii	AGOS_AFR355C	NP_985902.2
Arabidopsis thaliana (thale cress)	MCM3	NP_199440.1
Homo sapiens (human)	MCM3	NP_002379.3
	MCM3	XP_001106966.1
Gallus gallus (chicken)	MCM3	NP_001006421.1
Rattus norvegicus (Norway rat)	Mcm3	NP_001178734.2
Saccharomyces cerevisiae (baker's yeast)	MCM3	NP_010882.1
Schizosaccharomyces pombe (fission yeast)	mcm3	NP_587795.1
Magnaporthe oryzae (rice blast fungus)	MGG_01759	XP_003714758.1
Oryza sativa (rice)	Os05g0476200	NP_001055835.1
Pan troglodytes (chimpanzee)	MCM3	XP_001151750.2
Canis lupus familiaris (dog)	MCM3	XP_538960.3
Danio rerio (zebrafish)	mcm3	NP_997732.1
Drosophila melanogaster (fruit fly)	Mcm3	NP_511048.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001135	XP_322026.2
Kluyveromyces lactis	KLLA0E22243g	XP_454957.1
Neurospora crassa	NCU08009	XP_962679.1
Xenopus tropicalis (tropical clawed frog)	zmcm3	NP_001039231.1
Bos taurus (cattle)	MCM3	NP_001013604.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Leontovich AA, Intine RV, Sarras MP Jr
Journal of diabetes research20162860780(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Telomerase Is Essential for Zebrafish Heart Regeneration.
Bednarek D, Gonz?lez-Rosa JM, Guzm?n-Mart?nez G, Guti?rrez-Guti?rrez ?, Aguado T, S?nchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, G?mez MJ, Cort?s A, Zapata A, Jim?nez-Borreguero LJ, Mercader N, Flores I
Cell reports12(10)1691-703(2015 Sep)

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Jacob V, Chernyavskaya Y, Chen X, Tan PS, Kent B, Hoshida Y, Sadler KC
Development (Cambridge, England)142(3)510-21(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mcm3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcm3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53312	NM_212567.2 Latest version!	Danio rerio minichromosome maintenance complex component 3 (mcm3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcm3 ( NM_212567.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997732.2 Danio rerio minichromosome maintenance complex component 3 (mcm3), mRNA.

Price & Availability↑

CloneID	ODa53312
Clone ID Related Accession (Same CDS sequence)	NM_212567.2
Accession Version	NM_212567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA replication licensing factor MCM3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX279523.5. On May 4, 2016 this sequence version replaced NM_212567.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056718.1, GFIL01026088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG AAGTTGTGGA CGATCAGGAA ATGAGGGAGG CTCAGAGGGA TTACTTGGAT 
TTTCTTGACG ACGACCAAGA CCAGGGCATC TACCAGAGCA AAGTACGGCA GATGATCAGC 
GAAAACAAAT CCAGGCTCAT TGTAAACCTC AACGACCTGC GACGACGAAA TGAAAAGCGA 
GCAGCAAAGC TGTTGAATAA CGCTTTTGAA GAGCTCCTGG CATTCCAGCG AGCCCTGAAA 
GATCTGGTGG CTTCAATAGA TGCCACTTAC GCCAAGCAAT GTGAGGAGTT CTTCATTGGT 
TTGGAGGGCA GCTTTGGCAG CAAACATGTC TCCCCTCGAA CCCTGACCTC TCGCCTGCTG 
GGCAGCATGG TGTGTTTGGA GGGCATCGTC ACTAAATGCT CTTTGGTGCG TCCCAAAGTA 
GTACGCAGTG TGCACTACTG TCCAGCCACT AAAAAGACCA TGGAGCGCAA ATACACCGAT 
TTGACTTCTC TGGATGCCTT CCCTTCAAGT GCCATTTACC CCACCAAGGA TGAAGAGAAC 
AATCCATTGG AGACAGAGTT TGGTCTGTCT GTCTATAAGG ACCACCAAAC CATCACTATA 
CAGGAGATGC CGGAGAAGGC CCCTGCTGGT CAGCTGCCCC GCTCTGTGGA CATCATCCTG 
GACAATGACC TGGTGGACGC CGTGAAGCCT GGAGACCGAA CACAGGTGAT CGGCACCTAC 
CGCTGCCTGC CGGGCAAGAA GGGTGGCTTC ACCTCGGGCA CCTTCAGGAC CATCATGATT 
GCCTGCCATG TCAAACAGAT GAGCAAAGAA ATTTCCCACT ACTTCTCTGC GGATGATGTG 
GCCAAAATCA AGAGCTTCTG CAGGTCTCGC TCTAAGAATG TGTTTGATCA GTTGGCTCGC 
TCTCTGGCCC CCAGTATTCA CGGTCATGAG TACATAAAGA AGGCCATCCT GTGCATGCTT 
CTAGGAGGAG TGGAGAAGGT GCTGGAGAAC GGTTCGCGTA TTCGAGGAGA CATTAACGTG 
CTTCTCATAG GTGACCCATC AGTGGCCAAG TCTCAGTTGC TCCGGTACGT TCTTCACACT 
GCTCCTCGGG CCATTCCCAC AACAGGCCGT GGTTCATCAG GTGTGGGTCT GACCGCAGCC 
GTCACCACTG ACCAGGAGAC AGGCGAGCGG CGTCTGGAAG CTGGGGCCAT GGTTTTGGGG 
GATCGAGGCG TTGTCTGCAT TGACGAGTTC GACAAAATGT CAGATATGGA TCGCACTGCT 
ATACATGAAG TCATGGAGCA GGGCCGAGTC ACCATCGCTA AAGCTGGCAT CCACGCTAGA 
CTTAATGCCC GCTGCAGCGT CCTGGCAGCT GCCAACCCCG TCTACGGCAG ATATGATCAG 
TATAAGACAC CCATGGAGAA CATCGGTCTG CAGGACTCTC TGCTGTCTCG TTTTGATCTG 
CTCTTCATCA TGCTGGATCA GATGGACCCT GAGCAGGACC GCGAGATCTC GGACCACGTG 
CTGCGTATGC ACCGCTACAG AGATCCACAT GAGCAGGACG GGACAGCTCT TGCTCTTGGT 
GGTACAATCG ATGCTTTGGC CACGGAAGAC CCTGATGCTA CGCAGGAGGA GGAAGAGGAG 
CTCCAGGTTT ACGAGAAACA CAATCCACTC CTCCACGGCA GCAAAAAAAA CAAGGATCGA 
GTAGTGAGCA AAGCCTTCAT GAGGAAGTAC ATCCATGTCG CCAAAGCCAT TTCTCCAGTT 
TTGACCCAAG ATGCAGCCAA TCACATCGCA GAGGAATATT CACGACTGCG CAACCAGGAG 
CAGCTGGGCT CCGACATCGC ACGGACGTCA CCGGTCACTG CCCGAACCCT GGAGACTTTG 
ATCCGTCTGT CCACGGCTCA CGCTAAAGCC CGCATGAGTA AAGCTGTGGA GCTGGTGGAC 
ACTGAAGTCG CCGTGGAGCT CGTGCAGTTC GCTTATTTCA AGAAGGTTTT GGAGAAGGAA 
CGTAAACGCT CTAGAAATGG ACAGCAGGAT GCTGCTTCAG AAGACGAGGA AGAAGAGGAA 
GATGAAGCTC AAGACACACC ACGACCACCC AGAAAAAGGC GCAGGGGATC TCAGGGAAGC 
GAGTCTTATG ACCCCTATGA CTTCAACACT GAGACTGATA TTCCACAGAT TCAGTCTCCG 
GCTCCTGCGT TGCAGGCTGA TGAAGAGCCT GATCAGAATG GCCACACAGA GTTGTCTGAG 
GACAGGTTTA AGGAGTTTAA AGCGGCTCTG CATAAGGTTT TCCGCTCTTC TCACGCTCAG 
TCGGTGGGTT TGATTGCTCT GATGGAGAGC ATCAACAAGT CTTGCCCATC AGCGTTTAAT 
GAAACAGACT TCCGGGCTGC TCTCGCGCGC ATGCAGGACG ACAACCAGGT CATGCTGGCT 
GATGACATCA TCTTCCTGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997732.2
CDS	34..2457
Translation	MAAEVVDDQEMREAQRDYLDFLDDDQDQGIYQSKVRQMISENKSRLIVNLNDLRRRNEKRAAKLLNNAFEELLAFQRALKDLVASIDATYAKQCEEFFIGLEGSFGSKHVSPRTLTSRLLGSMVCLEGIVTKCSLVRPKVVRSVHYCPATKKTMERKYTDLTSLDAFPSSAIYPTKDEENNPLETEFGLSVYKDHQTITIQEMPEKAPAGQLPRSVDIILDNDLVDAVKPGDRTQVIGTYRCLPGKKGGFTSGTFRTIMIACHVKQMSKEISHYFSADDVAKIKSFCRSRSKNVFDQLARSLAPSIHGHEYIKKAILCMLLGGVEKVLENGSRIRGDINVLLIGDPSVAKSQLLRYVLHTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLGDRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRDPHEQDGTALALGGTIDALATEDPDATQEEEEELQVYEKHNPLLHGSKKNKDRVVSKAFMRKYIHVAKAISPVLTQDAANHIAEEYSRLRNQEQLGSDIARTSPVTARTLETLIRLSTAHAKARMSKAVELVDTEVAVELVQFAYFKKVLEKERKRSRNGQQDAASEDEEEEEDEAQDTPRPPRKRRRGSQGSESYDPYDFNTETDIPQIQSPAPALQADEEPDQNGHTELSEDRFKEFKAALHKVFRSSHAQSVGLIALMESINKSCPSAFNETDFRAALARMQDDNQVMLADDIIFLI

Target ORF information:

RefSeq Version	NM_212567.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio minichromosome maintenance complex component 3 (mcm3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212567.2

ORF Insert Sequence:

ATGGCTGCTG AAGTTGTGGA CGATCAGGAA ATGAGGGAGG CTCAGAGGGA TTACTTGGAT 
TTTCTTGACG ACGACCAAGA CCAGGGCATC TACCAGAGCA AAGTACGGCA GATGATCAGC 
GAAAACAAAT CCAGGCTCAT TGTAAACCTC AACGACCTGC GACGACGAAA TGAAAAGCGA 
GCAGCAAAGC TGTTGAATAA CGCTTTTGAA GAGCTCCTGG CATTCCAGCG AGCCCTGAAA 
GATCTGGTGG CTTCAATAGA TGCCACTTAC GCCAAGCAAT GTGAGGAGTT CTTCATTGGT 
TTGGAGGGCA GCTTTGGCAG CAAACATGTC TCCCCTCGAA CCCTGACCTC TCGCCTGCTG 
GGCAGCATGG TGTGTTTGGA GGGCATCGTC ACTAAATGCT CTTTGGTGCG TCCCAAAGTA 
GTACGCAGTG TGCACTACTG TCCAGCCACT AAAAAGACCA TGGAGCGCAA ATACACCGAT 
TTGACTTCTC TGGATGCCTT CCCTTCAAGT GCCATTTACC CCACCAAGGA TGAAGAGAAC 
AATCCATTGG AGACAGAGTT TGGTCTGTCT GTCTATAAGG ACCACCAAAC CATCACTATA 
CAGGAGATGC CGGAGAAGGC CCCTGCTGGT CAGCTGCCCC GCTCTGTGGA CATCATCCTG 
GACAATGACC TGGTGGACGC CGTGAAGCCT GGAGACCGAA CACAGGTGAT CGGCACCTAC 
CGCTGCCTGC CGGGCAAGAA GGGTGGCTTC ACCTCGGGCA CCTTCAGGAC CATCATGATT 
GCCTGCCATG TCAAACAGAT GAGCAAAGAA ATTTCCCACT ACTTCTCTGC GGATGATGTG 
GCCAAAATCA AGAGCTTCTG CAGGTCTCGC TCTAAGAATG TGTTTGATCA GTTGGCTCGC 
TCTCTGGCCC CCAGTATTCA CGGTCATGAG TACATAAAGA AGGCCATCCT GTGCATGCTT 
CTAGGAGGAG TGGAGAAGGT GCTGGAGAAC GGTTCGCGTA TTCGAGGAGA CATTAACGTG 
CTTCTCATAG GTGACCCATC AGTGGCCAAG TCTCAGTTGC TCCGGTACGT TCTTCACACT 
GCTCCTCGGG CCATTCCCAC AACAGGCCGT GGTTCATCAG GTGTGGGTCT GACCGCAGCC 
GTCACCACTG ACCAGGAGAC AGGCGAGCGG CGTCTGGAAG CTGGGGCCAT GGTTTTGGGG 
GATCGAGGCG TTGTCTGCAT TGACGAGTTC GACAAAATGT CAGATATGGA TCGCACTGCT 
ATACATGAAG TCATGGAGCA GGGCCGAGTC ACCATCGCTA AAGCTGGCAT CCACGCTAGA 
CTTAATGCCC GCTGCAGCGT CCTGGCAGCT GCCAACCCCG TCTACGGCAG ATATGATCAG 
TATAAGACAC CCATGGAGAA CATCGGTCTG CAGGACTCTC TGCTGTCTCG TTTTGATCTG 
CTCTTCATCA TGCTGGATCA GATGGACCCT GAGCAGGACC GCGAGATCTC GGACCACGTG 
CTGCGTATGC ACCGCTACAG AGATCCACAT GAGCAGGACG GGACAGCTCT TGCTCTTGGT 
GGTACAATCG ATGCTTTGGC CACGGAAGAC CCTGATGCTA CGCAGGAGGA GGAAGAGGAG 
CTCCAGGTTT ACGAGAAACA CAATCCACTC CTCCACGGCA GCAAAAAAAA CAAGGATCGA 
GTAGTGAGCA AAGCCTTCAT GAGGAAGTAC ATCCATGTCG CCAAAGCCAT TTCTCCAGTT 
TTGACCCAAG ATGCAGCCAA TCACATCGCA GAGGAATATT CACGACTGCG CAACCAGGAG 
CAGCTGGGCT CCGACATCGC ACGGACGTCA CCGGTCACTG CCCGAACCCT GGAGACTTTG 
ATCCGTCTGT CCACGGCTCA CGCTAAAGCC CGCATGAGTA AAGCTGTGGA GCTGGTGGAC 
ACTGAAGTCG CCGTGGAGCT CGTGCAGTTC GCTTATTTCA AGAAGGTTTT GGAGAAGGAA 
CGTAAACGCT CTAGAAATGG ACAGCAGGAT GCTGCTTCAG AAGACGAGGA AGAAGAGGAA 
GATGAAGCTC AAGACACACC ACGACCACCC AGAAAAAGGC GCAGGGGATC TCAGGGAAGC 
GAGTCTTATG ACCCCTATGA CTTCAACACT GAGACTGATA TTCCACAGAT TCAGTCTCCG 
GCTCCTGCGT TGCAGGCTGA TGAAGAGCCT GATCAGAATG GCCACACAGA GTTGTCTGAG 
GACAGGTTTA AGGAGTTTAA AGCGGCTCTG CATAAGGTTT TCCGCTCTTC TCACGCTCAG 
TCGGTGGGTT TGATTGCTCT GATGGAGAGC ATCAACAAGT CTTGCCCATC AGCGTTTAAT 
GAAACAGACT TCCGGGCTGC TCTCGCGCGC ATGCAGGACG ACAACCAGGT CATGCTGGCT 
GATGACATCA TCTTCCTGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Journal of diabetes research20162860780(2016)
Leontovich AA,Intine RV,Sarras MP Jr

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Telomerase Is Essential for Zebrafish Heart Regeneration.
Cell reports12(10)1691-703(2015 Sep)
Bednarek D,Gonz?lez-Rosa JM,Guzm?n-Mart?nez G,Guti?rrez-Guti?rrez ?,Aguado T,S?nchez-Ferrer C,Marques IJ,Galardi-Castilla M,de Diego I,G?mez MJ,Cort?s A,Zapata A,Jim?nez-Borreguero LJ,Mercader N,Flores I

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Development (Cambridge, England)142(3)510-21(2015 Feb)
Jacob V,Chernyavskaya Y,Chen X,Tan PS,Kent B,Hoshida Y,Sadler KC

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mcm3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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