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sema7a cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol sema7a
Entrez Gene ID 324910
Full Name semaphorin 7A
Synonyms fc47g07,wu:fc47g07,zgc:174881
General protein information
Preferred Names

semaphorin 7A

Names

semaphorin-7A
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001114885.2 NP_001108357.2 semaphorin-7A isoform 2
NM_001328508.1 NP_001315437.1 semaphorin-7A isoform 1

Pathways from BioSystems


Homologies


Bos taurus (cattle) SEMA7A NP_001193114.1
Homo sapiens (human) SEMA7A NP_003603.1
Gallus gallus (chicken) SEMA7A NP_001186678.1
Danio rerio (zebrafish) sema7a NP_001108357.1
SEMA7A XP_001099042.1
Canis lupus familiaris (dog) SEMA7A XP_005638673.1
Rattus norvegicus (Norway rat) Sema7a NP_001101623.1
Pan troglodytes (chimpanzee) SEMA7A XP_523219.2
Mus musculus (house mouse) Sema7a NP_035482.1
Xenopus tropicalis (tropical clawed frog) sema7a XP_002937527.2

Gene Ontology


Bibliography

Related articles in PubMed

Gene expression profiling of granule cells and Purkinje cells in the zebrafish cerebellum.
Takeuchi M, Yamaguchi S, Sakakibara Y, Hayashi T, Matsuda K, Hara Y, Tanegashima C, Shimizu T, Kuraku S, Hibi M
The Journal of comparative neurology525(7)1558-1585(2017 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sema7a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema7a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa29527 NM_001328508.1
Latest version!		 Danio rerio semaphorin 7A (sema7a), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
ODa53393 NM_001114885.2
Latest version!		 Danio rerio semaphorin 7A (sema7a), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa29527
Clone ID Related Accession (Same CDS sequence) NM_001328508.1
Accession Version NM_001328508.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-23
Organism Danio rerio(zebrafish)
Product semaphorin-7A isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR391977.5 and CR376804.6. On Jun 16, 2016 this sequence version replaced XM_005163112.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01006601.1, GDQQ01002660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA4476731 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATTCGAC ATTATTCTTC TCTCAAATTC AAAGGGAATT TGGCGTATCC TTGGCTTATT 
TATGGAGCGT GTTTTCAATG CGTGTTTTGC TCTTGTAATC CCAGATTAAC TCTGAAACAA 
GATAATGCTC CATTGAATTA CAACACCAAC TACAGTCATA CAGTTTTGTT CCATGAAGAA 
GGATCGAAAA GTTTATTTGT GGGAGGATCA AACATTGTTC TGCACTTTGA TGTAGACAGT 
TACGAGATTT TGGAGAATTT TACTCTAAAT GCAAACTTGC CAAGATGTGG TGAGAGCTCT 
TGTGAAAATG TAGTCACAGT AATTGAGACT TTTCAAAACT GCAAATTTGT ATGTGGGACA 
AATGGAGAAG AGCCACAGTG CTGGGAACTG TATCCAAGAG AAAGCAATCA TTCTGTTGTT 
AAGTCTGTAG AAGGCACAGG GATCTCGCCA CATTCTAGTG AACAAAACTC CTTGTCTCTT 
GTAGCAGATG GAGATCTATA TGCAGCAGCT CCTCTATACA GTGATGGAAC ACTTCTGCAG 
TTCCGTAGAA AAGCTGGAAA GCGAATCAAC GTATGGATGC ATGATCAGTG GGTCTCAGAA 
CCTACTTTTA TCTCCAGTTT CCTGGCCAAG CGCAAAAATG ACTCCTTGAA TGAGAAGATA 
TATGTCCTAT TCCGAGAAAA AAACTCAGAC ACTAGTCCAG AGGCGGATCC TTGGATATCT 
AGAGTTGCCA GAGTGTGTAA GATTGATGAG GGCGGTTCCA AACAGTTCCT CCAAAACATA 
TGGACCTCTT TCCTGAAGGC ACGACTTGTT TGTGGGATAC CAAAGGAGTC ACTGTATTTT 
AACCGCCTGC AAGATGTTTT TGTCCAGCAT GCTGAGGACT GGAGGGATTC ACTAGTTTAT 
GCACTCTTCT CCAGTAGCTG GAACTCTACC GCTGTCTGCA TTTACTCTTT AGCAGATCTT 
GATGACATAT TTGAGAAATC AAACTATAAA GGTTTTTCTG AGGCCATTCC CAACCCAAGA 
CCAGGAAAAT GTGTGGAAAA CAGCAATTCC CTTCCAATTC CTACTCTCAA AATTATTAAA 
GACTATCCAG AGATGAAGGA CTGGATACAT CCCATTCAAA AAGACACACC TTTCTTCACA 
AGTAGCAAGA ATTTTACCAA GATAGTTGTT GATCAAATTC GAGCTTTGGA TGAAAATGTA 
TACAATGTGC TGCTGCTTGC GACAGACGAT GGGATGATCT TCAAAATCCT ACAGCATGGC 
TCTAAACCTT TCATCATTTC TGAAACGCAT TTGTGCAATG GCTGTGCACC CATTCAATCA 
ATGAAACTTG ACTCTAAAAA GCGGAAGCTC TTTGTTGGAT ATCCTGGTCA AATGTCGGTG 
TTAGAACTAC AGAGATGCCA AGACTATGGT GCTTCTTGTG AAGAATGCGT TCTTTCTCGT 
GATCCATACT GCGCATGGTC TGAAAAAGGC TGCACGTCAC AAACTAGAGG AGGAATACAA 
AACATTTCTG ATGGCAATGT CACTAAAGTA TGTGACCACA AATCAGTGGG AAGAGTTAGA 
CGAGACGCTC AACAGTCATC TACTGCTCCC TCAAGGATTC AACACACTGT GGCCACTGAT 
GTCCCCTTCT ACTTATCTTG TCCGGTCGAT TCACATCATG CCAGTTATAG ATGGGAACAC 
GGGGGCAAAA GCAACCCATG TCAACAGACC CAATCGGAAT ACCTCCTCCT AATCCCAGCT 
ATGACGGCTG AGAATTATGG CAATTACAGT TGTGTTTCCC AGGAACGTGA TTACATCAAA 
GTTGTGAGAG AATATGAACT CCTTGCTAAG TCTGTTTTTA ATGATGCTTT CAAGCTGAAA 
ATACAAGACC GGCAGCTAAT GGTAGCATTT GTCACGAGTG CCTTCTATCT GTGCTCACTT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001315437.1
CDS90..2012
Translation

Target ORF information:

RefSeq Version NM_001328508.1
Organism Danio rerio(zebrafish)
Definition Danio rerio semaphorin 7A (sema7a), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001328508.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATTCGAC ATTATTCTTC TCTCAAATTC AAAGGGAATT TGGCGTATCC TTGGCTTATT 
TATGGAGCGT GTTTTCAATG CGTGTTTTGC TCTTGTAATC CCAGATTAAC TCTGAAACAA 
GATAATGCTC CATTGAATTA CAACACCAAC TACAGTCATA CAGTTTTGTT CCATGAAGAA 
GGATCGAAAA GTTTATTTGT GGGAGGATCA AACATTGTTC TGCACTTTGA TGTAGACAGT 
TACGAGATTT TGGAGAATTT TACTCTAAAT GCAAACTTGC CAAGATGTGG TGAGAGCTCT 
TGTGAAAATG TAGTCACAGT AATTGAGACT TTTCAAAACT GCAAATTTGT ATGTGGGACA 
AATGGAGAAG AGCCACAGTG CTGGGAACTG TATCCAAGAG AAAGCAATCA TTCTGTTGTT 
AAGTCTGTAG AAGGCACAGG GATCTCGCCA CATTCTAGTG AACAAAACTC CTTGTCTCTT 
GTAGCAGATG GAGATCTATA TGCAGCAGCT CCTCTATACA GTGATGGAAC ACTTCTGCAG 
TTCCGTAGAA AAGCTGGAAA GCGAATCAAC GTATGGATGC ATGATCAGTG GGTCTCAGAA 
CCTACTTTTA TCTCCAGTTT CCTGGCCAAG CGCAAAAATG ACTCCTTGAA TGAGAAGATA 
TATGTCCTAT TCCGAGAAAA AAACTCAGAC ACTAGTCCAG AGGCGGATCC TTGGATATCT 
AGAGTTGCCA GAGTGTGTAA GATTGATGAG GGCGGTTCCA AACAGTTCCT CCAAAACATA 
TGGACCTCTT TCCTGAAGGC ACGACTTGTT TGTGGGATAC CAAAGGAGTC ACTGTATTTT 
AACCGCCTGC AAGATGTTTT TGTCCAGCAT GCTGAGGACT GGAGGGATTC ACTAGTTTAT 
GCACTCTTCT CCAGTAGCTG GAACTCTACC GCTGTCTGCA TTTACTCTTT AGCAGATCTT 
GATGACATAT TTGAGAAATC AAACTATAAA GGTTTTTCTG AGGCCATTCC CAACCCAAGA 
CCAGGAAAAT GTGTGGAAAA CAGCAATTCC CTTCCAATTC CTACTCTCAA AATTATTAAA 
GACTATCCAG AGATGAAGGA CTGGATACAT CCCATTCAAA AAGACACACC TTTCTTCACA 
AGTAGCAAGA ATTTTACCAA GATAGTTGTT GATCAAATTC GAGCTTTGGA TGAAAATGTA 
TACAATGTGC TGCTGCTTGC GACAGACGAT GGGATGATCT TCAAAATCCT ACAGCATGGC 
TCTAAACCTT TCATCATTTC TGAAACGCAT TTGTGCAATG GCTGTGCACC CATTCAATCA 
ATGAAACTTG ACTCTAAAAA GCGGAAGCTC TTTGTTGGAT ATCCTGGTCA AATGTCGGTG 
TTAGAACTAC AGAGATGCCA AGACTATGGT GCTTCTTGTG AAGAATGCGT TCTTTCTCGT 
GATCCATACT GCGCATGGTC TGAAAAAGGC TGCACGTCAC AAACTAGAGG AGGAATACAA 
AACATTTCTG ATGGCAATGT CACTAAAGTA TGTGACCACA AATCAGTGGG AAGAGTTAGA 
CGAGACGCTC AACAGTCATC TACTGCTCCC TCAAGGATTC AACACACTGT GGCCACTGAT 
GTCCCCTTCT ACTTATCTTG TCCGGTCGAT TCACATCATG CCAGTTATAG ATGGGAACAC 
GGGGGCAAAA GCAACCCATG TCAACAGACC CAATCGGAAT ACCTCCTCCT AATCCCAGCT 
ATGACGGCTG AGAATTATGG CAATTACAGT TGTGTTTCCC AGGAACGTGA TTACATCAAA 
GTTGTGAGAG AATATGAACT CCTTGCTAAG TCTGTTTTTA ATGATGCTTT CAAGCTGAAA 
ATACAAGACC GGCAGCTAAT GGTAGCATTT GTCACGAGTG CCTTCTATCT GTGCTCACTT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa53393
Clone ID Related Accession (Same CDS sequence) NM_001114885.2
Accession Version NM_001114885.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-23
Organism Danio rerio(zebrafish)
Product semaphorin-7A isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR391977.5. On Jun 16, 2016 this sequence version replaced NM_001114885.1. Transcript Variant: This variant (2) lacks several exons, and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC155290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505392 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGATTCGAC ATTATTCTTC TCTCAAATTC AAAGGGAATT TGGCGTATCC TTGGCTTATT 
TATGGAGCGT GTTTTCAATG CGTGTTTTGC TCTTGTAATC CCAGATTAAC TCTGAAACAA 
GATAATGCTC CATTGAATTA CAACACCAAC TACAGTCATA CAGTTTTGTT CCATGAAGAA 
GGATCGAAAA GTTTATTTGT GGGAGGATCA AACATTGTTC TGCACTTTGA TGTAGACAGT 
TACGAGATTT TGGAGAATTT TACTCTAAAT GCAAACTTGC CAAGATGTGG TGAGAGCTCT 
TGTGAAAATG TAGTCACAGT AATTGAGACT TTTCAAAACT GCAAATTTGT ATGTGGGACA 
AATGGAGAAG AGCCACAGTG CTGGGAACTG TATCCAAGAG AAAGCAATCA TTCTGTTGTT 
AAGTCTGTAG AAGGCACAGG GATCTCGCCA CATTCTAGTG AACAAAACTC CTTGTCTCTT 
GTAGCAGATG GAGATCTATA TGCAGCAGCT CCTCTATACA GTGATGGAAC ACTTCTGCAG 
TTCCGTAGAA AAGCTGGAAA GCGAATCAAC GTATGGATGC ATGATCAGTG GGTCTCAGAA 
CCTACTTTTA TCTCCAGTTT CCTGGCCAAG CGCAAAAATG ACTCCTTGAA TGAGAAGATA 
TATGTCCTAT TCCGAGAAAA AAACTCAGAC ACTAGTCCAG AGGCGGATCC TTGGATATCT 
AGAGTTGCCA GAGTGTGTAA GATTGATGAG GGCGGTTCCA AACAGTTCCT CCAAAACATA 
TGGACCTCTT TCCTGAAGGC ACGACTTGTT TGTGGGATAC CAAAGGAGTC ACTGTATTTT 
AACCGCCTGC AAGATGTTTT TGTCCAGCAT GCTGAGGACT GGAGGGATTC ACTAGTTTAT 
GCACTCTTCT CCAGTAGCTG GAACTCTACC GCTGTCTGCA TTTACTCTTT AGCAGATCTT 
GATGACATAT TTGAGAAATC AAACTATAAA GGTTTTTCTG AGGCCATTCC CAACCCAAGA 
CCAGGAAAAT GTGTGGAAAA CAGCAATTCC CTTCCAATTC CTACTCTCAA AATTATTAAA 
GACTATCCAG AGATGAAGGA CTGGATACAT CCCATTCAAA AAGACACACC TTTCTTCACA 
AGTAGCAAGA ATTTTACCAA GATAGTTGTT GATCAAATTC GAGCTTTGGA TGAAAATGTA 
TACAATGTGC TGCTGCTTGC GACAGGTACA CATAGATGGT ATATGGGTTC AGTCTTTCTG 
TTCATTGATG TTTGCTTTGT GACTTTCTCC ACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001108357.2
CDS43..1338
Translation

Target ORF information:

RefSeq Version NM_001114885.2
Organism Danio rerio(zebrafish)
Definition Danio rerio semaphorin 7A (sema7a), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001114885.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGATTCGAC ATTATTCTTC TCTCAAATTC AAAGGGAATT TGGCGTATCC TTGGCTTATT 
TATGGAGCGT GTTTTCAATG CGTGTTTTGC TCTTGTAATC CCAGATTAAC TCTGAAACAA 
GATAATGCTC CATTGAATTA CAACACCAAC TACAGTCATA CAGTTTTGTT CCATGAAGAA 
GGATCGAAAA GTTTATTTGT GGGAGGATCA AACATTGTTC TGCACTTTGA TGTAGACAGT 
TACGAGATTT TGGAGAATTT TACTCTAAAT GCAAACTTGC CAAGATGTGG TGAGAGCTCT 
TGTGAAAATG TAGTCACAGT AATTGAGACT TTTCAAAACT GCAAATTTGT ATGTGGGACA 
AATGGAGAAG AGCCACAGTG CTGGGAACTG TATCCAAGAG AAAGCAATCA TTCTGTTGTT 
AAGTCTGTAG AAGGCACAGG GATCTCGCCA CATTCTAGTG AACAAAACTC CTTGTCTCTT 
GTAGCAGATG GAGATCTATA TGCAGCAGCT CCTCTATACA GTGATGGAAC ACTTCTGCAG 
TTCCGTAGAA AAGCTGGAAA GCGAATCAAC GTATGGATGC ATGATCAGTG GGTCTCAGAA 
CCTACTTTTA TCTCCAGTTT CCTGGCCAAG CGCAAAAATG ACTCCTTGAA TGAGAAGATA 
TATGTCCTAT TCCGAGAAAA AAACTCAGAC ACTAGTCCAG AGGCGGATCC TTGGATATCT 
AGAGTTGCCA GAGTGTGTAA GATTGATGAG GGCGGTTCCA AACAGTTCCT CCAAAACATA 
TGGACCTCTT TCCTGAAGGC ACGACTTGTT TGTGGGATAC CAAAGGAGTC ACTGTATTTT 
AACCGCCTGC AAGATGTTTT TGTCCAGCAT GCTGAGGACT GGAGGGATTC ACTAGTTTAT 
GCACTCTTCT CCAGTAGCTG GAACTCTACC GCTGTCTGCA TTTACTCTTT AGCAGATCTT 
GATGACATAT TTGAGAAATC AAACTATAAA GGTTTTTCTG AGGCCATTCC CAACCCAAGA 
CCAGGAAAAT GTGTGGAAAA CAGCAATTCC CTTCCAATTC CTACTCTCAA AATTATTAAA 
GACTATCCAG AGATGAAGGA CTGGATACAT CCCATTCAAA AAGACACACC TTTCTTCACA 
AGTAGCAAGA ATTTTACCAA GATAGTTGTT GATCAAATTC GAGCTTTGGA TGAAAATGTA 
TACAATGTGC TGCTGCTTGC GACAGGTACA CATAGATGGT ATATGGGTTC AGTCTTTCTG 
TTCATTGATG TTTGCTTTGT GACTTTCTCC ACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene expression profiling of granule cells and Purkinje cells in the zebrafish cerebellum.
The Journal of comparative neurology525(7)1558-1585(2017 May)
Takeuchi M,Yamaguchi S,Sakakibara Y,Hayashi T,Matsuda K,Hara Y,Tanegashima C,Shimizu T,Kuraku S,Hibi M


book

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R