hspa4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hspa4a
Entrez Gene ID	335865
Full Name	heat shock protein 4a
Synonyms	hspa4l,wu:fc41d05,wu:fi59h02,wu:fj35c08,zgc:55506
General protein information	Preferred Names heat shock protein 4a Names heat shock protein 4a heat shock protein 4, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214716.1	NP_999881.1	heat shock protein 4a

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HSPA4	XP_414655.2
Caenorhabditis elegans (roundworm)	C30C11.4	NP_498868.1
Saccharomyces cerevisiae (baker's yeast)	SSE1	NP_015219.1
Eremothecium gossypii	AGOS_AGR212W	NP_986878.1
Magnaporthe oryzae (rice blast fungus)	MGG_06065	XP_003711919.1
Arabidopsis thaliana (thale cress)	AT1G79920	NP_850984.4
Oryza sativa (rice)	Os05g0181000	NP_001054812.1
Homo sapiens (human)	HSPA4	NP_002145.3
Mus musculus (house mouse)	Hspa4	NP_032326.3
Danio rerio (zebrafish)	hspa4a	NP_999881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010331	XP_315285.3
Kluyveromyces lactis	KLLA0E24597g	XP_455059.1
Rattus norvegicus (Norway rat)	Hspa4	NP_705893.1
	HSPA4	XP_001106968.1
Danio rerio (zebrafish)	hspa4b	NP_956151.1
Drosophila melanogaster (fruit fly)	Hsc70Cb	NP_001189103.1
Schizosaccharomyces pombe (fission yeast)	pss1	XP_001713060.1
Xenopus tropicalis (tropical clawed frog)	hspa4	NP_989252.1
Pan troglodytes (chimpanzee)	HSPA4	XP_517930.2
Canis lupus familiaris (dog)	HSPA4	NP_001041481.1
Bos taurus (cattle)	HSPA4	NP_001107664.1
Neurospora crassa	NCU05269	XP_961759.2
Arabidopsis thaliana (thale cress)	HSP91	NP_178111.1
Oryza sativa (rice)	Os01g0180800	NP_001042210.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Jia XE, Ma K, Xu T, Gao L, Wu S, Fu C, Zhang W, Wang Z, Liu K, Dong M, Jing C, Ren C, Dong Z, Chen Y, Jin Y, Huang Q, Chang X, Deng M, Li L, Luo L, Zhu J, Dang Y, Chang HC, Zon LI, Zhou Y, Chen S, Pan W
Cell research25(8)946-62(2015 Aug)

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Feng H, Zhang YB, Zhang QM, Li Z, Zhang QY, Gui JF
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hspa4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hspa4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36496	NM_214716.1 Latest version!	Danio rerio heat shock protein 4a (hspa4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hspa4a ( NM_214716.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999881.1 Danio rerio heat shock protein 4a (hspa4a), mRNA.

Price & Availability↑

CloneID	ODa36496
Clone ID Related Accession (Same CDS sequence)	NM_214716.1
Accession Version	NM_214716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-22
Organism	Danio rerio(zebrafish)
Product	heat shock protein 4a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC051152.1. On Jul 6, 2005 this sequence version replaced XM_695894.1. ##Evidence-Data-START## Transcript exon combination :: BC051152.1, BC165369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGTGG TGGGGTTTGA TGTGGGTTTC CAGAGCTGTT ATGTTGCTGT GGCTCGGGCC 
GGTGGGATTG AAACCGTAGC AAACGAATAC AGCGACAGAT GTACACCGTC ATTTGTATCA 
TTCGGTCCTC GTAATCGGTC GATCGGGGCA GCTGCTAAAA GCCAGGTGGT CACAAACTGC 
AAGAACACTG TTCAGGGTTT TAAGCGATTC CATGGCAGGG CGTTCAGCGA CCCGTATGTG 
GAAACCACAC AGTCCAGTCT GGTCTATGAT CTTGCACAGA TGCCTAATGG AACCACCGGC 
ATTAAGGTCA TGTATATGGA GGAAGAGAAG CTGTTTGGCA TCGAGCAGGT CACTGCAATG 
CTGCTGACCA AACTGAAGGA GACCGCAGAA TCTGCACTCA AAAAGCCTGT GGCGGACTGT 
GTCATCTCTG TTCCCAGCTA TTTTACAGAT GCCGAGAGAA GATCAGTGAT GGATGCTGCA 
CAGATCGCAG GCCTCAACTG TCTCAGACTC ATGAATGACA CTACAGCAGT TGCACTGGCG 
TATGGCATCT ACAAACAGGA CCTGCCTGCT CCAGAGGAAA AGCCCAGGAC TGTTGTGTTT 
GTGGATGTTG GCCATGCTGG TTACCAGGTC TCTGCATGTG CCTTCAACAA AGGCAAGTTA 
AAGGTTCTGG GCTCTGCGTT TGACCCTGAG CTGGGCGGAA AGGACTTTGA CGAGGTGCTA 
GTGAAGCATT TCTGTGAGGA ATTTGCCCAG AAGTACAAGC TGGATGTGCG ATCGAAGCCC 
CGCGCTCTGG TTCGCCTTTA TCAGGAGTGC GAGAAGCTGA AAAAACTGAT GAGCGCAAAC 
TCCTCAGACC TGCCACTCAA CATCGAGTGC TTCATGAACG ACATCGATGT CAGCAGCAAA 
CTCAACAGAG CCAAGTTTGA GGAGCTTTGT GCGGGGCTGC TGGCCAAGGT GGAGGCTCCC 
TTGCAAAGCA TCATGGAACA AACCAGGCTG AAGAAAGAGG ACATCTATGC AGTGGAGATC 
ATCGGCGGCG CCTCCAGGAT TCCAGCCATT AAAGAGCGAA TCAGCAAGTT TTTTGGAAAG 
GAGTTGAGCA CCACGTTGAA TCTGGATGAA GCTGTGGCCA GAGGATGTGC ACTGCAGTGT 
GCAATCCTGT CGCCTGCTTT TAAAGTGCGC GAGTTCTCCA TCACAGACGT GGTGCCTTAC 
CCAATCTCTC TGAAGTGGAC TTCAGCAGCA GATGAAGGCG TCAGTGATTG TGAAGTTTTC 
CCAAAGAATC ACGCCGCACC CTTCTCGAAA GTGTTAACTT TCTACCGGAA GGAACCCTTC 
ACCTTGGAGG CCTACTACAA CAATCCTAAA GCCCTGCCCT ATCCCGATCC TACCATAGGT 
CAGTTTACCA TCCATAAGGT GGTTCCTCAG GCATCAGGAG AGAGCTCCAA AGTGAAAGTG 
AAGGTCCGAG TAAATGTTCA CGGGGTCTTC AGTGTTTCCA GCGCCTCATT GGTGGAACTG 
CTGAAACCCG GCGAGGGAGA AGAGCCAATG GAGACGGACA CCCCTGCCAA AGACGAAGAG 
AACAAGATGC AAGTGGATCA AGAGGCGCAG AAGGCTCAAG CTGATGATCA GAAAGAACAA 
GCTGACAAGA AATCAGACAC TGAAGACATG GAGACTTCAC CAGAAGACAA ACAGGAGAAG 
AAGAATGATC AGCCTCCACA GGCCAAGAAA GCCAAAGTGA AAACCAAGAC GGTGGATCTG 
CCCATCATGA ACAGCCTGCA GTGGCAGCTC GCCAGCGACG CGCTCAATCT CTTCATGGAG 
AACGAGGGGA AGATGATCAT GCAGGATAAA CTGGAGAAAG AAAGGAACGA TGCGAAGAAC 
TACGTGGAGG AATACGTTTA TGAAATGAGA GATAAACTGC ATGGAGTGCT GGAGAACTTT 
GTCAGCGAGG CGGAGCGCGA TTCTTTCTCC CTTAAACTGG AGGACACTGA AAACTGGTTG 
TATGAAGAAG GAGAGGACCA ACAGAAACAA GTGTACATTG ACAAACTGGC AGAGTTAAAG 
AAACTGGGTG ATCCTATTCA GAGCCGATAC ATTGAGGCGG AGGTGCGACC TAAAGCTTTC 
GAGGAGCTCG GAAGACAAAT CCAGCTGTAC ATGAAAGTTG TAGAGGCTTT TAAAGCAAAG 
GATGAGCTGT ATGATCATCT GGATGAGCTG GAGATGGTGA AGGTGGAGAA GCAGGTGAAT 
GATGCCATGA CCTGGATGAA CAACAAAATG AACCTGCAGA GCAAACAGAG TCTCAGCCAG 
GACCCCGCCG TTAAAGTGCA AGAGATCCAG ACCAAAACAA AGGAGCTGTA CTCCGCTTGC 
AATCCCGTTG TCACCAAATC CAAACCTAAA GTAGAGCCTC CAAAAGAGGA GACGGCAGCC 
GAACAGAACG GCCCCGTGAA CGGACAGCAG CCTTCAGACG CTCAGCCAGA GAAATCTCCA 
GAAAACACAG AGGCCAAACT TCCCGAAATG GACATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999881.1
CDS	185..2686
Translation	MSVVGFDVGFQSCYVAVARAGGIETVANEYSDRCTPSFVSFGPRNRSIGAAAKSQVVTNCKNTVQGFKRFHGRAFSDPYVETTQSSLVYDLAQMPNGTTGIKVMYMEEEKLFGIEQVTAMLLTKLKETAESALKKPVADCVISVPSYFTDAERRSVMDAAQIAGLNCLRLMNDTTAVALAYGIYKQDLPAPEEKPRTVVFVDVGHAGYQVSACAFNKGKLKVLGSAFDPELGGKDFDEVLVKHFCEEFAQKYKLDVRSKPRALVRLYQECEKLKKLMSANSSDLPLNIECFMNDIDVSSKLNRAKFEELCAGLLAKVEAPLQSIMEQTRLKKEDIYAVEIIGGASRIPAIKERISKFFGKELSTTLNLDEAVARGCALQCAILSPAFKVREFSITDVVPYPISLKWTSAADEGVSDCEVFPKNHAAPFSKVLTFYRKEPFTLEAYYNNPKALPYPDPTIGQFTIHKVVPQASGESSKVKVKVRVNVHGVFSVSSASLVELLKPGEGEEPMETDTPAKDEENKMQVDQEAQKAQADDQKEQADKKSDTEDMETSPEDKQEKKNDQPPQAKKAKVKTKTVDLPIMNSLQWQLASDALNLFMENEGKMIMQDKLEKERNDAKNYVEEYVYEMRDKLHGVLENFVSEAERDSFSLKLEDTENWLYEEGEDQQKQVYIDKLAELKKLGDPIQSRYIEAEVRPKAFEELGRQIQLYMKVVEAFKAKDELYDHLDELEMVKVEKQVNDAMTWMNNKMNLQSKQSLSQDPAVKVQEIQTKTKELYSACNPVVTKSKPKVEPPKEETAAEQNGPVNGQQPSDAQPEKSPENTEAKLPEMDID

Target ORF information:

RefSeq Version	NM_214716.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock protein 4a (hspa4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214716.1

ORF Insert Sequence:

ATGTCAGTGG TGGGGTTTGA TGTGGGTTTC CAGAGCTGTT ATGTTGCTGT GGCTCGGGCC 
GGTGGGATTG AAACCGTAGC AAACGAATAC AGCGACAGAT GTACACCGTC ATTTGTATCA 
TTCGGTCCTC GTAATCGGTC GATCGGGGCA GCTGCTAAAA GCCAGGTGGT CACAAACTGC 
AAGAACACTG TTCAGGGTTT TAAGCGATTC CATGGCAGGG CGTTCAGCGA CCCGTATGTG 
GAAACCACAC AGTCCAGTCT GGTCTATGAT CTTGCACAGA TGCCTAATGG AACCACCGGC 
ATTAAGGTCA TGTATATGGA GGAAGAGAAG CTGTTTGGCA TCGAGCAGGT CACTGCAATG 
CTGCTGACCA AACTGAAGGA GACCGCAGAA TCTGCACTCA AAAAGCCTGT GGCGGACTGT 
GTCATCTCTG TTCCCAGCTA TTTTACAGAT GCCGAGAGAA GATCAGTGAT GGATGCTGCA 
CAGATCGCAG GCCTCAACTG TCTCAGACTC ATGAATGACA CTACAGCAGT TGCACTGGCG 
TATGGCATCT ACAAACAGGA CCTGCCTGCT CCAGAGGAAA AGCCCAGGAC TGTTGTGTTT 
GTGGATGTTG GCCATGCTGG TTACCAGGTC TCTGCATGTG CCTTCAACAA AGGCAAGTTA 
AAGGTTCTGG GCTCTGCGTT TGACCCTGAG CTGGGCGGAA AGGACTTTGA CGAGGTGCTA 
GTGAAGCATT TCTGTGAGGA ATTTGCCCAG AAGTACAAGC TGGATGTGCG ATCGAAGCCC 
CGCGCTCTGG TTCGCCTTTA TCAGGAGTGC GAGAAGCTGA AAAAACTGAT GAGCGCAAAC 
TCCTCAGACC TGCCACTCAA CATCGAGTGC TTCATGAACG ACATCGATGT CAGCAGCAAA 
CTCAACAGAG CCAAGTTTGA GGAGCTTTGT GCGGGGCTGC TGGCCAAGGT GGAGGCTCCC 
TTGCAAAGCA TCATGGAACA AACCAGGCTG AAGAAAGAGG ACATCTATGC AGTGGAGATC 
ATCGGCGGCG CCTCCAGGAT TCCAGCCATT AAAGAGCGAA TCAGCAAGTT TTTTGGAAAG 
GAGTTGAGCA CCACGTTGAA TCTGGATGAA GCTGTGGCCA GAGGATGTGC ACTGCAGTGT 
GCAATCCTGT CGCCTGCTTT TAAAGTGCGC GAGTTCTCCA TCACAGACGT GGTGCCTTAC 
CCAATCTCTC TGAAGTGGAC TTCAGCAGCA GATGAAGGCG TCAGTGATTG TGAAGTTTTC 
CCAAAGAATC ACGCCGCACC CTTCTCGAAA GTGTTAACTT TCTACCGGAA GGAACCCTTC 
ACCTTGGAGG CCTACTACAA CAATCCTAAA GCCCTGCCCT ATCCCGATCC TACCATAGGT 
CAGTTTACCA TCCATAAGGT GGTTCCTCAG GCATCAGGAG AGAGCTCCAA AGTGAAAGTG 
AAGGTCCGAG TAAATGTTCA CGGGGTCTTC AGTGTTTCCA GCGCCTCATT GGTGGAACTG 
CTGAAACCCG GCGAGGGAGA AGAGCCAATG GAGACGGACA CCCCTGCCAA AGACGAAGAG 
AACAAGATGC AAGTGGATCA AGAGGCGCAG AAGGCTCAAG CTGATGATCA GAAAGAACAA 
GCTGACAAGA AATCAGACAC TGAAGACATG GAGACTTCAC CAGAAGACAA ACAGGAGAAG 
AAGAATGATC AGCCTCCACA GGCCAAGAAA GCCAAAGTGA AAACCAAGAC GGTGGATCTG 
CCCATCATGA ACAGCCTGCA GTGGCAGCTC GCCAGCGACG CGCTCAATCT CTTCATGGAG 
AACGAGGGGA AGATGATCAT GCAGGATAAA CTGGAGAAAG AAAGGAACGA TGCGAAGAAC 
TACGTGGAGG AATACGTTTA TGAAATGAGA GATAAACTGC ATGGAGTGCT GGAGAACTTT 
GTCAGCGAGG CGGAGCGCGA TTCTTTCTCC CTTAAACTGG AGGACACTGA AAACTGGTTG 
TATGAAGAAG GAGAGGACCA ACAGAAACAA GTGTACATTG ACAAACTGGC AGAGTTAAAG 
AAACTGGGTG ATCCTATTCA GAGCCGATAC ATTGAGGCGG AGGTGCGACC TAAAGCTTTC 
GAGGAGCTCG GAAGACAAAT CCAGCTGTAC ATGAAAGTTG TAGAGGCTTT TAAAGCAAAG 
GATGAGCTGT ATGATCATCT GGATGAGCTG GAGATGGTGA AGGTGGAGAA GCAGGTGAAT 
GATGCCATGA CCTGGATGAA CAACAAAATG AACCTGCAGA GCAAACAGAG TCTCAGCCAG 
GACCCCGCCG TTAAAGTGCA AGAGATCCAG ACCAAAACAA AGGAGCTGTA CTCCGCTTGC 
AATCCCGTTG TCACCAAATC CAAACCTAAA GTAGAGCCTC CAAAAGAGGA GACGGCAGCC 
GAACAGAACG GCCCCGTGAA CGGACAGCAG CCTTCAGACG CTCAGCCAGA GAAATCTCCA 
GAAAACACAG AGGCCAAACT TCCCGAAATG GACATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Cell research25(8)946-62(2015 Aug)
Jia XE,Ma K,Xu T,Gao L,Wu S,Fu C,Zhang W,Wang Z,Liu K,Dong M,Jing C,Ren C,Dong Z,Chen Y,Jin Y,Huang Q,Chang X,Deng M,Li L,Luo L,Zhu J,Dang Y,Chang HC,Zon LI,Zhou Y,Chen S,Pan W

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)
Feng H,Zhang YB,Zhang QM,Li Z,Zhang QY,Gui JF

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hspa4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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