ORF cDNA Clones and Custom Clones » Species Summary » Danio rerio » gjd2b cDNA ORF clone

gjd2b cDNA ORF clone, Danio rerio(zebrafish)

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Summary

Gene Symbol	gjd2b
Entrez Gene ID	378444
Full Name	gap junction protein delta 2b
Synonyms	connexin35,cx35,cx35b,gja9
General protein information	Preferred Names gap junction protein delta 2b Names gap junction delta-2 protein connexin 35 connexin 35b cx35.1 gap junction protein alpha 9
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_194420.1	NP_919401.1	gap junction delta-2 protein

Homologies

Homo sapiens (human)	GJD2	NP_065711.1
Mus musculus (house mouse)	Gjd2	NP_034420.2
Gallus gallus (chicken)	GJD2	NP_989913.1
Bos taurus (cattle)	GJD2	NP_777108.1
Rattus norvegicus (Norway rat)	Gjd2	NP_062154.1
Danio rerio (zebrafish)	cx35b	NP_919401.1
Canis lupus familiaris (dog)	GJD2	XP_544602.2
Pan troglodytes (chimpanzee)	GJD2	XP_510284.1
Xenopus tropicalis (tropical clawed frog)	gjd2	XP_002932880.1

Bibliography

Related articles in PubMed

A genetic basis for molecular asymmetry at vertebrate electrical synapses.
Miller AC, Whitebirch AC, Shah AN, Marsden KC, Granato M, O'Brien J, Moens CB
eLife6(2017 May)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Hippocampal-like circuitry in the pallium of an electric fish: Possible substrates for recursive pattern separation and completion.
Elliott SB, Harvey-Girard E, Giassi AC, Maler L
The Journal of comparative neurology525(1)8-46(2017 Jan)

Rapid reverse genetic screening using CRISPR in zebrafish.
Shah AN, Davey CF, Whitebirch AC, Miller AC, Moens CB
Nature methods12(6)535-40(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study detect cx35b mRNA in secondary motor neurons (SMNs) and interneurons in the spinal cord of Danio rerio embryos and larvae.
Title: Connexin 35b expression in the spinal cord of Danio rerio embryos and larvae.

Connexin 35 expression is first seen in the developing central nervous system at 1 day post fertilization.
Title: Distribution of the gap junction protein connexin 35 in the central nervous system of developing zebrafish larvae.

the magnitude of photoreceptor coupling is controlled by the dynamic phosphorylation and dephosphorylation of Cx35
Title: Photoreceptor coupling is controlled by connexin 35 phosphorylation in zebrafish retina.

The gamma hemichannel of Cx35 hemichannels exhibited a pronounced membrane potential dependence
Title: Biophysical characterization of zebrafish connexin35 hemichannels.

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The following gjd2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gjd2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02054	NM_194420.1 Latest version!	Danio rerio gap junction protein delta 2b (gjd2b), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gjd2b ( NM_194420.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919401.1 Danio rerio gap junction protein delta 2b (gjd2b), mRNA.

Price & Availability↑

CloneID	ODa02054
Clone ID Related Accession (Same CDS sequence)	NM_194420.1
Accession Version	NM_194420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	gap junction delta-2 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF462040.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF462040.1, BC162925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGAAT GGACAATTCT CGAGCGTCTC CTGGAGGCGG CTGTCCAACA GCACTCTACT 
ATGATTGGGA GGATCCTGCT AACTGTTGTG GTGATCTTCC GGATTCTAAT TGTGGCGATT 
GTTGGAGAGA CCGTGTACGA CGACGAGCAG TCAATGTTTG TGTGTAACAC TCTGCAGCCA 
GGCTGTAACC AAGCTTGCTA TGACAAAGCG TTCCCTATAT CTCACATCAG ATACTGGGTT 
TTCCAGATCA TCATGGTTTG CACACCCAGT CTCTGCTTCA TCACATACTC TGTGCATCAG 
TCGGCCAAAC AGAAGGAGCG GAGGTATTCT ACCATCTACC TGTCCCTCGA CAAAGACCCC 
GATACGATGA GGCGAGACGA CAGCAAAAAG ATCAAAAACA CCATTGTGAA CGGAGTACTT 
CAAAACACGG AGAACTCCAC CAAAGAGTCC GAGCCTGACT GTCTAGAGGT CAAAGAGATC 
CCCAATTCAG CCATGAGAAC TACCAAATCT AAAATGAGAA GACAAGAGGG CATCTCCAGG 
TTTTACATCA TTCAGGTGGT GTTCAGAAAC GCGTTGGAAA TTGGCTTCCT GGTGGGCCAG 
TATTTCTTGT ACGGATTCAA CGTGCCCGCC GTGTACGAGT GCGACCGCTA TCCTTGCATC 
AAAGACGTCG AATGCTATGT ATCAAGACCT ACAGAGAAAA CTGTGTTCCT CGTCTTCATG 
TTTGCTGTCA GTGGGATTTG CGTAGTGCTC AACCTGGCTG AACTCAATCA CCTGGGCTGG 
AGGAAAATTA AAACAGCGGT GAGGGGGGTT CAGGCCAGGA GGAAGTCCAT CTATGAGATC 
AGAAACAAGG ACTTACCGAG GATGAGCATG CCGAATTTCG GAAGAACCCA GTCCAGTGAC 
TCTGCCTACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919401.1
CDS	1..915
Translation	MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQSMFVCNTLQPGCNQACYDKAFPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQKERRYSTIYLSLDKDPDTMRRDDSKKIKNTIVNGVLQNTENSTKESEPDCLEVKEIPNSAMRTTKSKMRRQEGISRFYIIQVVFRNALEIGFLVGQYFLYGFNVPAVYECDRYPCIKDVECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKTAVRGVQARRKSIYEIRNKDLPRMSMPNFGRTQSSDSAYV

Target ORF information:

RefSeq Version	NM_194420.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gap junction protein delta 2b (gjd2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194420.1

ORF Insert Sequence:

ATGGGGGAAT GGACAATTCT CGAGCGTCTC CTGGAGGCGG CTGTCCAACA GCACTCTACT 
ATGATTGGGA GGATCCTGCT AACTGTTGTG GTGATCTTCC GGATTCTAAT TGTGGCGATT 
GTTGGAGAGA CCGTGTACGA CGACGAGCAG TCAATGTTTG TGTGTAACAC TCTGCAGCCA 
GGCTGTAACC AAGCTTGCTA TGACAAAGCG TTCCCTATAT CTCACATCAG ATACTGGGTT 
TTCCAGATCA TCATGGTTTG CACACCCAGT CTCTGCTTCA TCACATACTC TGTGCATCAG 
TCGGCCAAAC AGAAGGAGCG GAGGTATTCT ACCATCTACC TGTCCCTCGA CAAAGACCCC 
GATACGATGA GGCGAGACGA CAGCAAAAAG ATCAAAAACA CCATTGTGAA CGGAGTACTT 
CAAAACACGG AGAACTCCAC CAAAGAGTCC GAGCCTGACT GTCTAGAGGT CAAAGAGATC 
CCCAATTCAG CCATGAGAAC TACCAAATCT AAAATGAGAA GACAAGAGGG CATCTCCAGG 
TTTTACATCA TTCAGGTGGT GTTCAGAAAC GCGTTGGAAA TTGGCTTCCT GGTGGGCCAG 
TATTTCTTGT ACGGATTCAA CGTGCCCGCC GTGTACGAGT GCGACCGCTA TCCTTGCATC 
AAAGACGTCG AATGCTATGT ATCAAGACCT ACAGAGAAAA CTGTGTTCCT CGTCTTCATG 
TTTGCTGTCA GTGGGATTTG CGTAGTGCTC AACCTGGCTG AACTCAATCA CCTGGGCTGG 
AGGAAAATTA AAACAGCGGT GAGGGGGGTT CAGGCCAGGA GGAAGTCCAT CTATGAGATC 
AGAAACAAGG ACTTACCGAG GATGAGCATG CCGAATTTCG GAAGAACCCA GTCCAGTGAC 
TCTGCCTACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A genetic basis for molecular asymmetry at vertebrate electrical synapses.
eLife6(2017 May)
Miller AC,Whitebirch AC,Shah AN,Marsden KC,Granato M,O'Brien J,Moens CB

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Hippocampal-like circuitry in the pallium of an electric fish: Possible substrates for recursive pattern separation and completion.
The Journal of comparative neurology525(1)8-46(2017 Jan)
Elliott SB,Harvey-Girard E,Giassi AC,Maler L

Rapid reverse genetic screening using CRISPR in zebrafish.
Nature methods12(6)535-40(2015 Jun)
Shah AN,Davey CF,Whitebirch AC,Miller AC,Moens CB

Collapse all
Expand all

gjd2b cDNA ORF clone, Danio rerio(zebrafish)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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