ORF cDNA Clones and Custom Clones » Species Summary » Danio rerio » nelfb cDNA ORF clone

nelfb cDNA ORF clone, Danio rerio(zebrafish)

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Summary

Gene Symbol	nelfb
Entrez Gene ID	393137
Full Name	negative elongation factor complex member B
Synonyms	NELF-B,bZ1O1.4,cobra1,cobra2,fe01e12,wu:fe01e12,zgc:55628
General protein information	Preferred Names negative elongation factor complex member B Names negative elongation factor B cofactor of BRCA1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200168.1	NP_956462.1	negative elongation factor B

Homologies

	COBRA1	XP_001087434.1
Rattus norvegicus (Norway rat)	Nelfb	NP_001101287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012109	XP_320419.3
Homo sapiens (human)	NELFB	NP_056271.2
Bos taurus (cattle)	NELFB	XP_002691742.3
Xenopus tropicalis (tropical clawed frog)	nelfb	NP_001011448.1
Mus musculus (house mouse)	Nelfb	NP_067368.2
Gallus gallus (chicken)	COBRA1	NP_001006202.1
Pan troglodytes (chimpanzee)	COBRA1	XP_001136264.3
Canis lupus familiaris (dog)	NELFB	XP_548351.2
Danio rerio (zebrafish)	nelfb	NP_956462.1
Drosophila melanogaster (fruit fly)	NELF-B	NP_572402.1

Bibliography

Related articles in PubMed

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Yang Q, Liu X, Zhou T, Cook J, Nguyen K, Bai X
Blood128(13)1701-10(2016 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of retinoid-X receptor genes rxra, rxrba, rxrbb and rxrg during zebrafish development.
Tallafuss A, Hale LA, Yan YL, Dudley L, Eisen JS, Postlethwait JH
Gene expression patterns : GEP6(5)556-65(2006 Jun)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following nelfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nelfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35972	NM_200168.1 Latest version!	Danio rerio negative elongation factor complex member B (nelfb), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nelfb ( NM_200168.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956462.1 Danio rerio negative elongation factor complex member B (nelfb), mRNA.

Price & Availability↑

CloneID	ODa35972
Clone ID Related Accession (Same CDS sequence)	NM_200168.1
Accession Version	NM_200168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	negative elongation factor B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045409.1. On or before Jun 13, 2012 this sequence version replaced NM_001020786.1, XM_679656.1, XM_702544.1. ##Evidence-Data-START## Transcript exon combination :: BC045409.1, BC164663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTCGCCG GGCTACCGGA GCTGGGAATA TCCAATGGAG AGGATCTGAA AGAAACTCTG 
ACTAACTGTA CTGAACCTCA CAAAGCAATC GACCAGTTTC AGACGGAAAA TGGCATATTG 
CTGCCAACCC TACAGTCTGC ATTGCCTTTT CTGGATCTTC ACGGGACTCC ACGGCTGGAG 
TTTCATCAGT CGGTCTTTGA TGAACTTCGA GACAAGCTAA TGGAGCGTGT TGCCACCATT 
GCAGAGGGCA AGGAAGAGGA CAGGTACGTA AAGCTGGAGG AACTACTGGA GAAAAGCTTT 
CCCCTAGTGA AGATGCCATC CATACAGCCG GTTGTCATGC AAGTTCTGAA GCATCTGCCA 
AAGGTGCCTG AGAAGAAGCT GAAGCAAGTG ATGGCGGATA AAGAGCTGTA CAAAGTCTGC 
GCGGTGGAGG TGAAACGGCA AATCTGGCAG GACAATCAGG CTCTTTTTGG AGACGAGGTG 
TCTCCTCTCT TGAAACAGTA TATTGTGGAG AAGGAAGCAG CTCTTTTTAG CAATGACCTC 
TCTATCCTGC ACAACTTTTT CAGCCCTTCA CCCAAAACAC GGCGACAGGG GGAGGTGGTG 
CTGAAGCTGA CTCAGATGAT TGGGAAGAAC GTGAAGCTCT ATGATATGGT GCTGCAGTTC 
TTGCGAACGC TGTTTCTGCG TACACGTAAT GTACATTACC GCACCTTGCG AGCGGAGCTG 
CTCATGTCCC TTCATGACCT CGACATCAGC GAGATCTGTT CAGTCGACCC CTGTCACAAG 
TTTTCCTGGT GTTTGGATGC CTGCATCAGG GAGAAGTTTG TGGATGCTAA ACGGGCCCGA 
GAGTTACAAG GCTTTTTGGA TGGTGTGAAG AAAGGCCAGG AGCAAGTGTT AGGTGACCTT 
TCTATGATCC TCTGTGACCC CTTTGCCTGT AACACACTGG TGTTGAGCAC TGTTAGGAAC 
CTTCAGGAGT TGTTGAGTCA GGACGCCCTG CCCAGAGACA GTCCTGACCT TCTCCTCCTG 
CTGCGGATGC TCTCTCTGGG GCAGGGCGCC TGGGACATGA TCGACAGTCA GGTTTTCAAA 
GAGCCTCGAA TGGATCTGGA GGTGGTGACA CGCTTTCTGC CTGCCATGAT GTCGATAGTG 
GTGGACGACT ACACGTTTAC TGTGGAGCAG AAACTGCCGA GCGAAGAGAA GACTTCTCTC 
GCGTACCCTA CCACTCTGCC TGAAACCTTC TCCAGGTATA TACTGGATAA CAGGGTGGCG 
TGTGAGATTG GCCTTTACTA TGCTCTGCAT ATTGCCAAGC TGAGGAATAA AAATGCTTTA 
CAGCGGCTTC TTCCTGCATT GGTGGATACA TATAATGACA TGGCATTCGG AGATATATTT 
CTCCATCTGC TCACTGGTCA TCTGACTCTT CTCTCTGATG AGTTTGGAAC AGAGGGGTTT 
TGTACTGCTG TCTTTGATAA CTTCCTACTC ACGTCTTTCT CAAGTAAGGA GAACGTTCAC 
AGGCATTGCC TTCGCTTGCT GAATCATCTC CATCAGAAGG TGCTGCCGAC TTACATGGAA 
ACCTTGATGA AGACGCTTGA ACCACCAAAA CAGAGCAGTG AGTCGGTGAA GGAGCTGTAC 
AACTTGCTGA AGGAGAAGCT GGAGGCCTCT AAGAAAAGCC CCCCGCAACC AGAGGAAGCC 
CCTTCTCTAG ATCTCGGCCT TCATCCTGTT ACTGTGCCGC CCACTCCCTC CACCCCCACA 
ACCCCTGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956462.1
CDS	170..1921
Translation	MFAGLPELGISNGEDLKETLTNCTEPHKAIDQFQTENGILLPTLQSALPFLDLHGTPRLEFHQSVFDELRDKLMERVATIAEGKEEDRYVKLEELLEKSFPLVKMPSIQPVVMQVLKHLPKVPEKKLKQVMADKELYKVCAVEVKRQIWQDNQALFGDEVSPLLKQYIVEKEAALFSNDLSILHNFFSPSPKTRRQGEVVLKLTQMIGKNVKLYDMVLQFLRTLFLRTRNVHYRTLRAELLMSLHDLDISEICSVDPCHKFSWCLDACIREKFVDAKRARELQGFLDGVKKGQEQVLGDLSMILCDPFACNTLVLSTVRNLQELLSQDALPRDSPDLLLLLRMLSLGQGAWDMIDSQVFKEPRMDLEVVTRFLPAMMSIVVDDYTFTVEQKLPSEEKTSLAYPTTLPETFSRYILDNRVACEIGLYYALHIAKLRNKNALQRLLPALVDTYNDMAFGDIFLHLLTGHLTLLSDEFGTEGFCTAVFDNFLLTSFSSKENVHRHCLRLLNHLHQKVLPTYMETLMKTLEPPKQSSESVKELYNLLKEKLEASKKSPPQPEEAPSLDLGLHPVTVPPTPSTPTTPV

Target ORF information:

RefSeq Version	NM_200168.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio negative elongation factor complex member B (nelfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200168.1

ORF Insert Sequence:

ATGTTCGCCG GGCTACCGGA GCTGGGAATA TCCAATGGAG AGGATCTGAA AGAAACTCTG 
ACTAACTGTA CTGAACCTCA CAAAGCAATC GACCAGTTTC AGACGGAAAA TGGCATATTG 
CTGCCAACCC TACAGTCTGC ATTGCCTTTT CTGGATCTTC ACGGGACTCC ACGGCTGGAG 
TTTCATCAGT CGGTCTTTGA TGAACTTCGA GACAAGCTAA TGGAGCGTGT TGCCACCATT 
GCAGAGGGCA AGGAAGAGGA CAGGTACGTA AAGCTGGAGG AACTACTGGA GAAAAGCTTT 
CCCCTAGTGA AGATGCCATC CATACAGCCG GTTGTCATGC AAGTTCTGAA GCATCTGCCA 
AAGGTGCCTG AGAAGAAGCT GAAGCAAGTG ATGGCGGATA AAGAGCTGTA CAAAGTCTGC 
GCGGTGGAGG TGAAACGGCA AATCTGGCAG GACAATCAGG CTCTTTTTGG AGACGAGGTG 
TCTCCTCTCT TGAAACAGTA TATTGTGGAG AAGGAAGCAG CTCTTTTTAG CAATGACCTC 
TCTATCCTGC ACAACTTTTT CAGCCCTTCA CCCAAAACAC GGCGACAGGG GGAGGTGGTG 
CTGAAGCTGA CTCAGATGAT TGGGAAGAAC GTGAAGCTCT ATGATATGGT GCTGCAGTTC 
TTGCGAACGC TGTTTCTGCG TACACGTAAT GTACATTACC GCACCTTGCG AGCGGAGCTG 
CTCATGTCCC TTCATGACCT CGACATCAGC GAGATCTGTT CAGTCGACCC CTGTCACAAG 
TTTTCCTGGT GTTTGGATGC CTGCATCAGG GAGAAGTTTG TGGATGCTAA ACGGGCCCGA 
GAGTTACAAG GCTTTTTGGA TGGTGTGAAG AAAGGCCAGG AGCAAGTGTT AGGTGACCTT 
TCTATGATCC TCTGTGACCC CTTTGCCTGT AACACACTGG TGTTGAGCAC TGTTAGGAAC 
CTTCAGGAGT TGTTGAGTCA GGACGCCCTG CCCAGAGACA GTCCTGACCT TCTCCTCCTG 
CTGCGGATGC TCTCTCTGGG GCAGGGCGCC TGGGACATGA TCGACAGTCA GGTTTTCAAA 
GAGCCTCGAA TGGATCTGGA GGTGGTGACA CGCTTTCTGC CTGCCATGAT GTCGATAGTG 
GTGGACGACT ACACGTTTAC TGTGGAGCAG AAACTGCCGA GCGAAGAGAA GACTTCTCTC 
GCGTACCCTA CCACTCTGCC TGAAACCTTC TCCAGGTATA TACTGGATAA CAGGGTGGCG 
TGTGAGATTG GCCTTTACTA TGCTCTGCAT ATTGCCAAGC TGAGGAATAA AAATGCTTTA 
CAGCGGCTTC TTCCTGCATT GGTGGATACA TATAATGACA TGGCATTCGG AGATATATTT 
CTCCATCTGC TCACTGGTCA TCTGACTCTT CTCTCTGATG AGTTTGGAAC AGAGGGGTTT 
TGTACTGCTG TCTTTGATAA CTTCCTACTC ACGTCTTTCT CAAGTAAGGA GAACGTTCAC 
AGGCATTGCC TTCGCTTGCT GAATCATCTC CATCAGAAGG TGCTGCCGAC TTACATGGAA 
ACCTTGATGA AGACGCTTGA ACCACCAAAA CAGAGCAGTG AGTCGGTGAA GGAGCTGTAC 
AACTTGCTGA AGGAGAAGCT GGAGGCCTCT AAGAAAAGCC CCCCGCAACC AGAGGAAGCC 
CCTTCTCTAG ATCTCGGCCT TCATCCTGTT ACTGTGCCGC CCACTCCCTC CACCCCCACA 
ACCCCTGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Blood128(13)1701-10(2016 09)
Yang Q,Liu X,Zhou T,Cook J,Nguyen K,Bai X

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of retinoid-X receptor genes rxra, rxrba, rxrbb and rxrg during zebrafish development.
Gene expression patterns : GEP6(5)556-65(2006 Jun)
Tallafuss A,Hale LA,Yan YL,Dudley L,Eisen JS,Postlethwait JH

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Collapse all
Expand all

nelfb cDNA ORF clone, Danio rerio(zebrafish)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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