rars2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	rars2
Entrez Gene ID	393589
Full Name	arginyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms	CH73-317M8.1,zgc:63702
General protein information	Preferred Names arginyl-tRNA synthetase 2, mitochondrial (putative) Names probable arginine--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294528.2	XP_009292803.1	probable arginine--tRNA ligase, mitochondrial isoform X1
XM_009294528.3	XP_009292803.1	probable arginine--tRNA ligase, mitochondrial isoform X1
NM_200617.1	NP_956911.1	probable arginine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RARS2	NP_001069346.1
Mus musculus (house mouse)	Rars2	NP_852071.2
Gallus gallus (chicken)	RARS2	NP_001264948.1
Danio rerio (zebrafish)	rars2	NP_956911.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010086	XP_319244.3
Schizosaccharomyces pombe (fission yeast)	mrs1	NP_596077.1
Xenopus tropicalis (tropical clawed frog)	rars2	XP_002933968.2
Homo sapiens (human)	RARS2	NP_064716.2
Pan troglodytes (chimpanzee)	RARS2	XP_518630.2
	RARS2	XP_001092575.2
Canis lupus familiaris (dog)	RARS2	XP_539032.2
Kluyveromyces lactis	KLLA0A07040g	XP_451312.1
Eremothecium gossypii	AGOS_AFL206C	NP_985344.1
Rattus norvegicus (Norway rat)	Rars2	NP_001100113.1
Drosophila melanogaster (fruit fly)	CG10092	NP_001262351.1
Saccharomyces cerevisiae (baker's yeast)	YDR341C	NP_010628.3
Magnaporthe oryzae (rice blast fungus)	MGG_09080	XP_003711182.1
Neurospora crassa	NCU08195	XP_959285.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Impairment of the tRNA-splicing endonuclease subunit 54 (tsen54) gene causes neurological abnormalities and larval death in zebrafish models of pontocerebellar hypoplasia.
Kasher PR, Namavar Y, van Tijn P, Fluiter K, Sizarov A, Kamermans M, Grierson AJ, Zivkovic D, Baas F
Human molecular genetics20(8)1574-84(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37239	NM_200617.1 Latest version!	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (rars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40474	XM_009294528.3 Latest version!	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa40474	XM_009294528.2	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rars2 ( NM_200617.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956911.1 Danio rerio arginyl-tRNA synthetase 2, mitochondrial (rars2), mRNA.

Price & Availability↑

CloneID	ODa37239
Clone ID Related Accession (Same CDS sequence)	NM_200617.1
Accession Version	NM_200617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Danio rerio(zebrafish)
Product	probable arginine--tRNA ligase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056803.1. ##Evidence-Data-START## Transcript exon combination :: BC056803.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
ATCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCGGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CAGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTGGGGAACG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGTTCT CAGGAGAAGC TAAGGACGCA ACCACTAGAG 
CATCTGTTTG AGTCATCTGT TGGTGGATGC TGTGTGTATC TGTATGGCTC AGCTGTGTGT 
GTCCGCCCGC AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTG GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGATG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCA GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAAGAGGCG 
CAGAGCCGGA TGATCCACAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAACTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA TGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CAGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCGCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCACTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956911.1
CDS	81..1874
Translation	MACFFRRSAALQLAKACGQNEETLIPLISVAPVNKKQSVADLRVSVSHLLQSGAVRPDGDLQNTTHTLARQIRTDAVVEEIIPGRGVIHFRLNKLLLAQKLLDQLRSDPDGFGVKSEILRQLRVGRTLVEFSSPNIAKKFHAGHLRSTIIGNFIANLKKALGNEVIRVNYLGDWGMQFGLLGSGFERFGSQEKLRTQPLEHLFESSVGGCCVYLYGSAVCVRPQVYVQVNREAESDASVRSAAAHFFRRLEQSEEQALELWRHFREITIHEYKRIYQRLGVEFDHYSGESFHRAETQTVLDELRSRCLLKTSQKGTGVVDLSDKGDMSNYATLVRSDGTSLYITRDVAAALDRKQRFSFDEMIYVTDKSQTMHFQQLFRILQAMGHQWAGSCHHVAFGLVQGMSTRRGEVLFLEDVLEEAQSRMIHNMRQSRTSKELEDPELTAERIGISALIVQDFKGPLISDYRFEWDKVLQAQGDTGVFLQYTHARLCSLLRQQDCEISCSDFSPLTDSRSVSLLQHLLRYDEVLLQCATDLQPKHLVNFLMTLSHLVASAHRELPVKGSSEAVAQARLCLFSSVHSVLANGMRILGITPVDKM

Target ORF information:

RefSeq Version	NM_200617.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (rars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200617.1

ORF Insert Sequence:

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
ATCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCGGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CAGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTGGGGAACG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGTTCT CAGGAGAAGC TAAGGACGCA ACCACTAGAG 
CATCTGTTTG AGTCATCTGT TGGTGGATGC TGTGTGTATC TGTATGGCTC AGCTGTGTGT 
GTCCGCCCGC AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTG GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGATG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCA GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAAGAGGCG 
CAGAGCCGGA TGATCCACAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAACTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA TGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CAGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCGCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCACTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rars2 ( XM_009294528.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292803.1 Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40474
Clone ID Related Accession (Same CDS sequence)	XM_009294528.2 , XM_009294528.3
Accession Version	XM_009294528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable arginine--tRNA ligase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
GTCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCAGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CGGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTAGGGAATG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGCTCT CAGGAGAAGC TAAGGACGCA ACCACTGGAG 
CATCTGTTTG AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTT GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGACG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCC GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAGGAGGCG 
CAGAGCCGGA TGATCCATAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAAGTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA CGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CTGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCTCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCGCTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292803.1
CDS	89..1822
Translation	MACFFRRSAALQLAKACGQNEETLIPLISVAPVNKKQSVADLRVSVSHLLQSGAVRPDGDLQNTTHTLARQIRTDAVVEEVIPGRGVIHFRLNKLLLAQKLLDQLRSDPDGFGVKSEILRRLRVGRTLVEFSSPNIAKKFHAGHLRSTIIGNFIANLKKALGNEVIRVNYLGDWGMQFGLLGSGFERFGSQEKLRTQPLEHLFEVYVQVNREAESDASVRSAAAHFFRRLEQSEEQALELWRHFREITIHEYKRIYQRLGVEFDHYSGESFHRAETQTVLDELRSRRLLKTSQKGTGVVDLSDKGDMSNYATLVRSDGTSLYITRDVAAALDRKQRFSFDEMIYVTDKSQTMHFQQLFRILQAMGHPWAGSCHHVAFGLVQGMSTRRGEVLFLEDVLEEAQSRMIHNMRQSRTSKELEDPEVTAERIGISALIVQDFKGPLISDYRFEWDKVLQAQGDTGVFLQYTHARLCSLLRQQDCEISCSDFSPLTDSRSVSLLQHLLRYDEVLLQCATDLQPKHLVNFLMTLSHLVASAHRELPVKGSSEAVAQARLCLFSSVRSVLANGMRILGITPVDKM

Target ORF information:

RefSeq Version	XM_009294528.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294528.2

ORF Insert Sequence:

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
GTCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCAGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CGGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTAGGGAATG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGCTCT CAGGAGAAGC TAAGGACGCA ACCACTGGAG 
CATCTGTTTG AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTT GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGACG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCC GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAGGAGGCG 
CAGAGCCGGA TGATCCATAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAAGTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA CGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CTGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCTCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCGCTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rars2 ( XM_009294528.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292803.1 Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40474
Clone ID Related Accession (Same CDS sequence)	XM_009294528.2 , XM_009294528.3
Accession Version	XM_009294528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable arginine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
GTCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCAGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CGGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTAGGGAATG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGCTCT CAGGAGAAGC TAAGGACGCA ACCACTGGAG 
CATCTGTTTG AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTT GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGACG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCC GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAGGAGGCG 
CAGAGCCGGA TGATCCATAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAAGTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA CGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CTGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCTCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCGCTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292803.1
CDS	104..1837
Translation	MACFFRRSAALQLAKACGQNEETLIPLISVAPVNKKQSVADLRVSVSHLLQSGAVRPDGDLQNTTHTLARQIRTDAVVEEVIPGRGVIHFRLNKLLLAQKLLDQLRSDPDGFGVKSEILRRLRVGRTLVEFSSPNIAKKFHAGHLRSTIIGNFIANLKKALGNEVIRVNYLGDWGMQFGLLGSGFERFGSQEKLRTQPLEHLFEVYVQVNREAESDASVRSAAAHFFRRLEQSEEQALELWRHFREITIHEYKRIYQRLGVEFDHYSGESFHRAETQTVLDELRSRRLLKTSQKGTGVVDLSDKGDMSNYATLVRSDGTSLYITRDVAAALDRKQRFSFDEMIYVTDKSQTMHFQQLFRILQAMGHPWAGSCHHVAFGLVQGMSTRRGEVLFLEDVLEEAQSRMIHNMRQSRTSKELEDPEVTAERIGISALIVQDFKGPLISDYRFEWDKVLQAQGDTGVFLQYTHARLCSLLRQQDCEISCSDFSPLTDSRSVSLLQHLLRYDEVLLQCATDLQPKHLVNFLMTLSHLVASAHRELPVKGSSEAVAQARLCLFSSVRSVLANGMRILGITPVDKM

Target ORF information:

RefSeq Version	XM_009294528.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio arginyl-tRNA synthetase 2, mitochondrial (putative) (rars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294528.3

ORF Insert Sequence:

ATGGCGTGTT TTTTCAGGAG GAGTGCAGCT CTGCAGCTGG CGAAGGCCTG TGGTCAGAAT 
GAGGAGACGC TCATCCCACT GATCTCTGTG GCGCCGGTCA ACAAGAAACA GAGTGTGGCA 
GATCTGCGTG TGTCAGTCAG TCATCTTCTG CAGAGCGGAG CTGTTCGTCC TGATGGAGAC 
CTGCAGAACA CCACACACAC GCTGGCCCGA CAGATCAGAA CGGATGCTGT GGTGGAGGAG 
GTCATCCCCG GCAGAGGAGT GATTCACTTC AGACTCAACA AACTCCTCCT GGCGCAGAAA 
CTTTTAGACC AGCTGCGCTC AGATCCAGAC GGCTTCGGTG TGAAGAGTGA AATCCTGCGC 
CGGCTTCGGG TCGGCCGGAC ACTGGTGGAG TTCAGTTCTC CAAACATCGC CAAGAAGTTC 
CACGCTGGAC ATCTCAGATC CACCATAATC GGAAATTTCA TTGCCAACCT AAAGAAGGCT 
TTAGGGAATG AGGTGATCCG AGTCAATTAT CTGGGCGACT GGGGAATGCA GTTCGGTCTG 
TTGGGCTCAG GGTTTGAACG ATTCGGCTCT CAGGAGAAGC TAAGGACGCA ACCACTGGAG 
CATCTGTTTG AGGTGTATGT GCAGGTGAAC CGCGAGGCGG AGAGCGATGC CAGTGTGCGT 
TCGGCTGCTG CTCATTTCTT CAGGCGTCTT GAGCAGAGCG AGGAGCAGGC GCTGGAGCTG 
TGGAGACACT TCAGGGAGAT CACCATCCAT GAGTACAAGC GCATCTATCA GCGTTTAGGA 
GTGGAGTTTG ATCATTACTC CGGAGAATCT TTTCATCGAG CTGAAACACA GACTGTGCTG 
GACGAGCTGA GGAGCAGACG CCTGCTGAAG ACCTCACAGA AGGGAACGGG TGTGGTGGAT 
CTGTCTGATA AGGGCGACAT GTCCAATTAC GCCACACTGG TCCGCAGTGA TGGGACATCC 
CTGTACATCA CCAGAGACGT GGCAGCGGCG CTGGACAGGA AGCAGAGGTT TAGCTTTGAT 
GAGATGATCT ACGTGACAGA TAAGAGTCAG ACTATGCACT TCCAGCAGCT CTTCAGAATC 
TTACAGGCCA TGGGGCACCC GTGGGCCGGC AGTTGTCATC ATGTGGCGTT CGGCCTGGTG 
CAGGGCATGA GCACTCGACG TGGGGAGGTG CTGTTCCTGG AGGACGTGCT GGAGGAGGCG 
CAGAGCCGGA TGATCCATAA CATGAGGCAG TCCCGCACTT CTAAAGAGCT GGAGGATCCG 
GAAGTGACCG CAGAGAGAAT CGGGATCAGT GCTTTAATCG TACAGGACTT CAAAGGGCCG 
CTGATTTCTG ACTACAGGTT TGAATGGGAC AAAGTCCTGC AGGCTCAAGG AGACACGGGT 
GTGTTTCTGC AGTACACACA CGCTCGCCTC TGCAGTTTAT TGCGGCAGCA GGACTGCGAG 
ATCTCCTGCT CTGACTTCTC TCCTCTAACA GACAGCAGGA GTGTGTCTCT CCTCCAGCAC 
CTGCTCAGGT ATGATGAAGT GCTGCTTCAG TGTGCAACTG ACCTGCAGCC CAAACACCTG 
GTCAACTTCC TCATGACTCT AAGTCATCTA GTGGCATCGG CTCATCGAGA GCTGCCTGTG 
AAGGGTAGTT CAGAAGCGGT GGCGCAGGCC AGACTGTGTC TGTTCAGCTC CGTCCGCTCA 
GTTCTGGCCA ATGGCATGAG GATTCTGGGC ATCACTCCGG TTGATAAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Impairment of the tRNA-splicing endonuclease subunit 54 (tsen54) gene causes neurological abnormalities and larval death in zebrafish models of pontocerebellar hypoplasia.
Human molecular genetics20(8)1574-84(2011 Apr)
Kasher PR,Namavar Y,van Tijn P,Fluiter K,Sizarov A,Kamermans M,Grierson AJ,Zivkovic D,Baas F

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rars2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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