papola cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	papola
Entrez Gene ID	404041
Full Name	poly(A) polymerase alpha
Synonyms	papolb,si:ch211-199l3.6,wu:fc43b06,wu:fp01g10,zgc:109706
General protein information	Preferred Names poly(A) polymerase alpha Names poly(A) polymerase alpha poly(A) polymerase beta (testis specific)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294541.1	XP_009292816.1	poly(A) polymerase alpha isoform X2
NM_205758.2	NP_991321.2	poly(A) polymerase alpha
XM_005160349.1	XP_005160406.1	poly(A) polymerase alpha isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PAPOLA	NP_788820.1
Rattus norvegicus (Norway rat)	Papola	NP_001101526.1
Gallus gallus (chicken)	PAPOLA	NP_001185530.1
Oryza sativa (rice)	Os06g0558700	NP_001174846.1
	PAPOLA	XP_002805246.1
Mus musculus (house mouse)	Papola	NP_035242.1
Caenorhabditis elegans (roundworm)	pap-1	NP_505683.1
Caenorhabditis elegans (roundworm)	T15H9.6	NP_496067.1
Danio rerio (zebrafish)	papola	NP_991321.2
Caenorhabditis elegans (roundworm)	CELE_F43G6.5	NP_496524.1
Arabidopsis thaliana (thale cress)	PAPS2	NP_001031417.1
Oryza sativa (rice)	Os02g0227400	NP_001046352.2
Xenopus tropicalis (tropical clawed frog)	papola	NP_001136382.1
Homo sapiens (human)	PAPOLA	NP_116021.2
Pan troglodytes (chimpanzee)	PAPOLA	XP_003314562.1
Canis lupus familiaris (dog)	PAPOLA	XP_005623838.1
Arabidopsis thaliana (thale cress)	nPAP	NP_974668.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Zhou S, Zhao H, Thongda W, Zhang D, Su B, Yu D, Peatman E, Li C
Fish & shellfish immunology49324-35(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following papola gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the papola cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36524	NM_205758.2 Latest version!	Danio rerio poly(A) polymerase alpha (papola), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
ODa24629	XM_005160349.1 Latest version!	Danio rerio poly(A) polymerase alpha (papola), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
ODa40773	XM_009294541.1 Latest version!	Danio rerio poly(A) polymerase alpha (papola), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

papola ( NM_205758.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991321.2 Danio rerio poly(A) polymerase alpha (papola), mRNA.

Price & Availability↑

CloneID	ODa36524
Clone ID Related Accession (Same CDS sequence)	NM_205758.2
Accession Version	NM_205758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	poly(A) polymerase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL953887.10. On Jan 5, 2007 this sequence version replaced NM_205758.1. ##Evidence-Data-START## Transcript exon combination :: GDQH01007699.1, GFIL01029448.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACC TGTCAGCCCC AGAAGCAAAG TCCTCAGGTG AAACTGATGG CCATGTGGCT 
CCAGCGATCG TTGAAGTGAT TCCTTCAGAC GGAGAATTGA AAACAGAGAC TGAATTAATG 
GTGGAGGACA CAGCAGAGAA GGTTCCACTT GCTCAGAAAA CATCCAACGC TGATCTATCG 
GACGTCCCGG TCCTCCCAAG CAACCCCATC CCTGTGGTCA AGAATTCGAT CAAGCTCCGA 
CTCAGCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991321.2
CDS	172..2343
Translation	MPFPITSQVQTAQPTPKHYGITSPISLAPPKEADLVLTRKLTETLRPFGVFEEELELQRRILVLGKLNTLVKFWIREVSEAKNIPASVIDSVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERTDFFSSFYEKLKEQEEVKDLRAVEEAFVPVIKLAFDGIEIDILFARLALQTIPENLDLRDDGLLKNLDIRCIRSLNGCRVTDEILHLVPNIENFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAVAATLVHKFFLVFSKWEWPNPVLLKQPEDCNLNLPVWDPRVTPSDRYHLMPIITPAYPQQNSTYNVSASTRAIMVEEFKRGLAITDEILQNKADWSKLFEAPNFFQKYKHYIVLLASAQTEKQHLEWVGLVESKIRILVGNLEKNEFITLAHVNPQSFPGPKEGNDKEEFSTMWVIGIVFKKMEGSENLNVDLTFDIQSFTDTVYRQAITSKMFEQDMKITAMHVKRKQLHQLLPEVAIHRSKRKHSSDGVRAMNDSNLDLSLDSDNSMSVPSPTGPSAAKIARAPSPQGSTGTATNTSELQGKTEATGENTHLASVDGTSSSNASGKGAAPPQSTSPIQSEKKPSEQNPLPSPCLSAEPAVKRPASALENEMTKKAKVEDLSAPEAKSSGETDGHVAPAIVEVIPSDGELKTETELMVEDTAEKVPLAQKTSNADLSDVPVLPSNPIPVVKNSIKLRLSR

Target ORF information:

RefSeq Version	NM_205758.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio poly(A) polymerase alpha (papola), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205758.2

ORF Insert Sequence:

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACC TGTCAGCCCC AGAAGCAAAG TCCTCAGGTG AAACTGATGG CCATGTGGCT 
CCAGCGATCG TTGAAGTGAT TCCTTCAGAC GGAGAATTGA AAACAGAGAC TGAATTAATG 
GTGGAGGACA CAGCAGAGAA GGTTCCACTT GCTCAGAAAA CATCCAACGC TGATCTATCG 
GACGTCCCGG TCCTCCCAAG CAACCCCATC CCTGTGGTCA AGAATTCGAT CAAGCTCCGA 
CTCAGCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

papola ( XM_005160349.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160406.1 Danio rerio poly(A) polymerase alpha (papola), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24629
Clone ID Related Accession (Same CDS sequence)	XM_005160349.1
Accession Version	XM_005160349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	poly(A) polymerase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACC TGTCAGCCCC AGAAGCAAAG TCCTCAGGTG AAACTGATGG CCATGTGGCT 
CCAGCGCAGA TCGTTGAAGT GATTCCTTCA GACGGAGAAT TGAAAACAGA GACTGAATTA 
ATGGTGGAGG ACACAGCAGA GAAGGTTCCA CTTGCTCAGA AAACATCCAA CGCTGATCTA 
TCGGACGTCC CGGTCCTCCC AAGCAACCCC ATCCCTGTGG TCAAGAATTC GATCAAGCTC 
CGACTCAGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160406.1
CDS	180..2354
Translation	MPFPITSQVQTAQPTPKHYGITSPISLAPPKEADLVLTRKLTETLRPFGVFEEELELQRRILVLGKLNTLVKFWIREVSEAKNIPASVIDSVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERTDFFSSFYEKLKEQEEVKDLRAVEEAFVPVIKLAFDGIEIDILFARLALQTIPENLDLRDDGLLKNLDIRCIRSLNGCRVTDEILHLVPNIENFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAVAATLVHKFFLVFSKWEWPNPVLLKQPEDCNLNLPVWDPRVTPSDRYHLMPIITPAYPQQNSTYNVSASTRAIMVEEFKRGLAITDEILQNKADWSKLFEAPNFFQKYKHYIVLLASAQTEKQHLEWVGLVESKIRILVGNLEKNEFITLAHVNPQSFPGPKEGNDKEEFSTMWVIGIVFKKMEGSENLNVDLTFDIQSFTDTVYRQAITSKMFEQDMKITAMHVKRKQLHQLLPEVAIHRSKRKHSSDGVRAMNDSNLDLSLDSDNSMSVPSPTGPSAAKIARAPSPQGSTGTATNTSELQGKTEATGENTHLASVDGTSSSNASGKGAAPPQSTSPIQSEKKPSEQNPLPSPCLSAEPAVKRPASALENEMTKKAKVEDLSAPEAKSSGETDGHVAPAQIVEVIPSDGELKTETELMVEDTAEKVPLAQKTSNADLSDVPVLPSNPIPVVKNSIKLRLSR

Target ORF information:

RefSeq Version	XM_005160349.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio poly(A) polymerase alpha (papola), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160349.1

ORF Insert Sequence:

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACC TGTCAGCCCC AGAAGCAAAG TCCTCAGGTG AAACTGATGG CCATGTGGCT 
CCAGCGCAGA TCGTTGAAGT GATTCCTTCA GACGGAGAAT TGAAAACAGA GACTGAATTA 
ATGGTGGAGG ACACAGCAGA GAAGGTTCCA CTTGCTCAGA AAACATCCAA CGCTGATCTA 
TCGGACGTCC CGGTCCTCCC AAGCAACCCC ATCCCTGTGG TCAAGAATTC GATCAAGCTC 
CGACTCAGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

papola ( XM_009294541.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292816.1 Danio rerio poly(A) polymerase alpha (papola), transcript variant X2, mRNA.

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CloneID	ODa40773
Clone ID Related Accession (Same CDS sequence)	XM_009294541.1
Accession Version	XM_009294541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	poly(A) polymerase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACA TCGTTGAAGT GATTCCTTCA GACGGAGAAT TGAAAACAGA GACTGAATTA 
ATGGTGGAGG ACACAGCAGA GAAGGTTCCA CTTGCTCAGA AAACATCCAA CGCTGATCTA 
TCGGACGTCC CGGTCCTCCC AAGCAACCCC ATCCCTGTGG TCAAGAATTC GATCAAGCTC 
CGACTCAGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292816.1
CDS	180..2294
Translation	MPFPITSQVQTAQPTPKHYGITSPISLAPPKEADLVLTRKLTETLRPFGVFEEELELQRRILVLGKLNTLVKFWIREVSEAKNIPASVIDSVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERTDFFSSFYEKLKEQEEVKDLRAVEEAFVPVIKLAFDGIEIDILFARLALQTIPENLDLRDDGLLKNLDIRCIRSLNGCRVTDEILHLVPNIENFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAVAATLVHKFFLVFSKWEWPNPVLLKQPEDCNLNLPVWDPRVTPSDRYHLMPIITPAYPQQNSTYNVSASTRAIMVEEFKRGLAITDEILQNKADWSKLFEAPNFFQKYKHYIVLLASAQTEKQHLEWVGLVESKIRILVGNLEKNEFITLAHVNPQSFPGPKEGNDKEEFSTMWVIGIVFKKMEGSENLNVDLTFDIQSFTDTVYRQAITSKMFEQDMKITAMHVKRKQLHQLLPEVAIHRSKRKHSSDGVRAMNDSNLDLSLDSDNSMSVPSPTGPSAAKIARAPSPQGSTGTATNTSELQGKTEATGENTHLASVDGTSSSNASGKGAAPPQSTSPIQSEKKPSEQNPLPSPCLSAEPAVKRPASALENEMTKKAKVEDIVEVIPSDGELKTETELMVEDTAEKVPLAQKTSNADLSDVPVLPSNPIPVVKNSIKLRLSR

Target ORF information:

RefSeq Version	XM_009294541.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio poly(A) polymerase alpha (papola), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294541.1

ORF Insert Sequence:

ATGCCTTTTC CAATTACCAG CCAGGTGCAA ACTGCCCAGC CGACCCCAAA ACATTATGGG 
ATCACATCTC CTATCAGCTT GGCACCGCCT AAAGAGGCTG ATCTGGTTCT CACGCGGAAG 
CTGACAGAGA CTCTCCGACC TTTCGGCGTC TTTGAGGAGG AGCTGGAGCT GCAGCGCAGA 
ATCCTGGTGC TCGGGAAGTT AAATACATTG GTTAAGTTTT GGATCCGGGA AGTCAGTGAA 
GCGAAGAATA TTCCAGCTTC AGTGATTGAC AGTGTTGGTG GAAAGATCTT CACGTTTGGA 
TCCTACAGGC TTGGAGTTCA TACCAAGGGT GCTGATATTG ATGCTCTCTG TGTCGCACCC 
CGCCATGTGG AAAGAACCGA CTTCTTCTCA TCATTTTATG AGAAGTTAAA AGAACAGGAG 
GAAGTCAAAG ATCTGAGGGC AGTGGAAGAA GCTTTTGTGC CTGTGATAAA GCTGGCTTTT 
GATGGAATTG AGATTGACAT ACTGTTTGCC AGACTGGCAC TACAAACCAT TCCAGAGAAC 
TTGGATTTGC GAGATGATGG CTTACTGAAG AACCTTGACA TTCGCTGTAT CCGGAGTTTG 
AACGGTTGCA GAGTCACTGA TGAGATTCTT CACCTGGTGC CCAACATAGA GAATTTCCGA 
CTGACTCTTA GAGCCATCAA ACTGTGGGCC AAACGTCACA ATATCTACTC CAATATCCTG 
GGTTTTCTCG GGGGTGTATC GTGGGCCATG CTAGTGGCAA GAACCTGCCA GCTGTACCCC 
AATGCTGTGG CAGCCACACT GGTCCATAAG TTCTTTTTGG TTTTCTCAAA GTGGGAATGG 
CCAAACCCAG TCCTTCTGAA GCAGCCTGAA GACTGTAACC TCAACCTCCC TGTCTGGGAC 
CCCAGGGTAA CCCCCAGTGA CCGGTACCAC CTCATGCCAA TCATCACACC AGCTTACCCT 
CAGCAAAACT CCACTTACAA CGTTTCAGCC TCCACTCGGG CCATCATGGT GGAAGAGTTC 
AAGCGGGGTC TCGCAATCAC GGATGAAATT TTGCAGAATA AAGCAGATTG GTCCAAACTA 
TTTGAAGCAC CAAACTTCTT TCAGAAATAC AAGCATTATA TAGTGTTGCT AGCAAGCGCA 
CAGACAGAGA AGCAGCATTT AGAGTGGGTT GGCCTTGTGG AATCAAAGAT CCGAATCCTC 
GTGGGAAACC TGGAGAAGAA TGAATTCATT ACACTCGCTC ATGTGAATCC ACAGTCATTC 
CCGGGACCAA AAGAGGGAAA TGACAAAGAG GAGTTTAGCA CAATGTGGGT AATAGGTATT 
GTGTTCAAAA AAATGGAGGG ATCAGAAAAT CTTAATGTGG ACCTGACGTT TGACATCCAG 
TCCTTCACAG ACACAGTTTA TCGTCAAGCG ATCACTAGTA AAATGTTTGA GCAAGATATG 
AAAATCACAG CCATGCATGT CAAGAGGAAA CAGCTGCATC AACTACTGCC CGAAGTCGCC 
ATTCACAGGA GCAAGAGAAA GCATTCATCT GATGGTGTTC GTGCAATGAA TGACAGCAAT 
CTGGACCTTT CACTTGACAG CGACAACAGC ATGTCAGTTC CCTCCCCAAC GGGCCCTTCA 
GCTGCAAAGA TAGCCCGAGC CCCCAGCCCT CAGGGATCCA CTGGGACCGC CACAAACACC 
TCAGAACTGC AGGGAAAAAC AGAGGCTACT GGCGAAAACA CACACCTGGC TAGTGTTGAT 
GGGACCTCCA GCAGCAATGC CTCTGGAAAA GGAGCGGCCC CTCCTCAGTC TACTTCACCT 
ATTCAGTCTG AAAAGAAACC CTCTGAACAG AACCCTCTGC CCAGTCCATG TTTATCTGCT 
GAACCTGCTG TTAAGAGACC TGCCTCTGCC CTCGAGAATG AGATGACCAA GAAAGCCAAA 
GTAGAGGACA TCGTTGAAGT GATTCCTTCA GACGGAGAAT TGAAAACAGA GACTGAATTA 
ATGGTGGAGG ACACAGCAGA GAAGGTTCCA CTTGCTCAGA AAACATCCAA CGCTGATCTA 
TCGGACGTCC CGGTCCTCCC AAGCAACCCC ATCCCTGTGG TCAAGAATTC GATCAAGCTC 
CGACTCAGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Fish & shellfish immunology49324-35(2016 Feb)
Zhou S,Zhao H,Thongda W,Zhang D,Su B,Yu D,Peatman E,Li C

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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papola cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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