ORF » Species Summary » Danio rerio » SPAST cDNA ORF clone

SPAST cDNA ORF clone, Danio rerio(Zebrafish)

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa06190 XM_005155397.3
Latest version!		 Danio rerio spastin (spast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 Quote Price
ODa98506 NM_212915.2
Latest version!		 Danio rerio spastin (spast), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 Quote Price
View Old Accession Versions >>
ODa06190 XM_005155397.2 Danio rerio spastin (spast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPAST ( XM_005155397.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155454.1 Danio rerio spastin (spast), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa06190
    Clone ID Related Accession (Same CDS sequence) XM_005155397.3 , XM_005155397.2
    Accession Version XM_005155397.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
    GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
    CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
    GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
    AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
    AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
    AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
    CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
    GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
    GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
    TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
    CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
    CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
    GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
    GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
    CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
    AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
    GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
    CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
    AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
    ACTGGCGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155454.1
    CDS140..1771
    Translation

    Target ORF information:

    RefSeq Version XM_005155397.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastin (spast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005155397.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
    GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
    CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
    GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
    AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
    AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
    AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
    CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
    GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
    GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
    TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
    CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
    CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
    GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
    GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
    CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
    AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
    GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
    CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
    AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
    ACTGGCGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPAST ( XM_005155397.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155454.1 Danio rerio spastin (spast), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa06190
    Clone ID Related Accession (Same CDS sequence) XM_005155397.3 , XM_005155397.2
    Accession Version XM_005155397.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spastin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005155397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
    GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
    CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
    GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
    AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
    AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
    AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
    CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
    GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
    GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
    TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
    CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
    CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
    GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
    GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
    CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
    AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
    GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
    CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
    AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
    ACTGGCGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155454.1
    CDS121..1752
    Translation

    Target ORF information:

    RefSeq Version XM_005155397.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spastin (spast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005155397.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
    GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
    CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
    GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
    AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
    AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
    AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
    CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
    GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
    GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
    TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
    CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
    CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
    GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
    GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
    CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
    AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
    GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
    CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
    AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
    ACTGGCGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPAST ( NM_212915.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_998080.2 Danio rerio spastin (spast), mRNA.
    Price & Availability↑
    CloneID ODa98506
    Clone ID Related Accession (Same CDS sequence) NM_212915.2
    Accession Version NM_212915.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Danio rerio(zebrafish)
    Product spastin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY304504.1 and CU651563.7. On Oct 9, 2016 this sequence version replaced NM_212915.1. ##Evidence-Data-START## Transcript exon combination :: AY304504.1, BC067715.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGGCAAT 
    CTGCTTTCAC AGTCTCCAGC AGAGAGCAGC TCTGATGATT CTTTCTACTC TTTCTCCAAT 
    GGTAATCTGC GTCCAGCTCC AGCCAGTGGA GCAGTCTCCA AGAAGAAGGA CACTCTCACA 
    ATCACAAATC AAACTTCTCT GCGGCCCAAA AATCCTCCCA AATCTACCCC GAATGCATCA 
    GGTCTGAACT GCACTCCCTC AGCAGCTCAG AGCAGCAGGA CCGGCCCTCA AAACAATCAG 
    AAGGGGCCCA CAGTCAAAGG CAAAAATAAT GTTAAAGCCA GTACCACTGC CACTGCGTCA 
    CCTCAAAGGA AAAGAGACAT GAAGAACTTC AAAAATGTGG ACAGCAAACT GGCAAGCCTC 
    ATCCTCAACG AGATCGTGGA CAGCGGGTCT GTGGTGAGGT TTGATGATAT AGCAGGACAG 
    GATTTGGCCA AGCAGGCGCT GCAGGAAATC GTCATCCTGC CTGCCCTACG TCCAGAGTTG 
    TTTACTGGTC TCCGGGCTCC AGCTAGAGGA CTCCTGCTCT TCGGCCCTCC TGGTAATGGG 
    AAAACCATGC TGGCGAAAGC CGTCGCAATG GAATCGAATG CTACTTTCTT CAACATCAGC 
    GCTGCCACTC TCACCTCCAA ATATGTGGGA GAGGGAGAGA AGCTGGTTCG AGCTCTGTTT 
    GCGGTCGCTC GAGAGCTGCA GCCGTCGATT ATCTTCATCG ATGAGATCGA CAGTCTGCTG 
    TGTGAGAGGA GGGAAGGCGA ACACGATGCC AGCAGACGAC TCAAAACAGA GTTTCTCATC 
    GAGTTTGATG GAGTGCAGTC AGGTGGAGAT GAGCGTGTGT TAGTGATGGG AGCCACTAAC 
    AGGCCTCAGG AGCTGGACGA GGCTGTTCTC AGGAGATTTG CTAAACGCAT TTATGTGGCT 
    TTACCTACAG AGGAGACTCG ACTGAAGCTG CTGAAGAATC TCCTCAGTAA ACACAGAAAT 
    CCTCTGTCTC AGAAAGAGCT CAGTCAGCTG GCCAGGCTGA CCGACGGTTA CTCTGGCAGC 
    GATCTCACCT CTCTGGCTAA AGACGCTGCT CTCGGCCCGA TACGAGAGCT GAAGCCGGAG 
    CAGGTGAGGA ACATGTCAGC CCATGAGATG CGTGACATTC GCATCTCTGA TTTCCTGGAG 
    TCTCTGAAGC GGATCAAGCG GAGCGTCAGT CCGCAGACCT TGGATCAGTA CGTGCGCTGG 
    AACCGCGAGT ACGGAGACAC GACTGGCGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_998080.2
    CDS119..1831
    Translation

    Target ORF information:

    RefSeq Version NM_212915.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastin (spast), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_212915.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
    GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
    TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
    ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
    CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
    GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
    AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
    CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGGCAAT 
    CTGCTTTCAC AGTCTCCAGC AGAGAGCAGC TCTGATGATT CTTTCTACTC TTTCTCCAAT 
    GGTAATCTGC GTCCAGCTCC AGCCAGTGGA GCAGTCTCCA AGAAGAAGGA CACTCTCACA 
    ATCACAAATC AAACTTCTCT GCGGCCCAAA AATCCTCCCA AATCTACCCC GAATGCATCA 
    GGTCTGAACT GCACTCCCTC AGCAGCTCAG AGCAGCAGGA CCGGCCCTCA AAACAATCAG 
    AAGGGGCCCA CAGTCAAAGG CAAAAATAAT GTTAAAGCCA GTACCACTGC CACTGCGTCA 
    CCTCAAAGGA AAAGAGACAT GAAGAACTTC AAAAATGTGG ACAGCAAACT GGCAAGCCTC 
    ATCCTCAACG AGATCGTGGA CAGCGGGTCT GTGGTGAGGT TTGATGATAT AGCAGGACAG 
    GATTTGGCCA AGCAGGCGCT GCAGGAAATC GTCATCCTGC CTGCCCTACG TCCAGAGTTG 
    TTTACTGGTC TCCGGGCTCC AGCTAGAGGA CTCCTGCTCT TCGGCCCTCC TGGTAATGGG 
    AAAACCATGC TGGCGAAAGC CGTCGCAATG GAATCGAATG CTACTTTCTT CAACATCAGC 
    GCTGCCACTC TCACCTCCAA ATATGTGGGA GAGGGAGAGA AGCTGGTTCG AGCTCTGTTT 
    GCGGTCGCTC GAGAGCTGCA GCCGTCGATT ATCTTCATCG ATGAGATCGA CAGTCTGCTG 
    TGTGAGAGGA GGGAAGGCGA ACACGATGCC AGCAGACGAC TCAAAACAGA GTTTCTCATC 
    GAGTTTGATG GAGTGCAGTC AGGTGGAGAT GAGCGTGTGT TAGTGATGGG AGCCACTAAC 
    AGGCCTCAGG AGCTGGACGA GGCTGTTCTC AGGAGATTTG CTAAACGCAT TTATGTGGCT 
    TTACCTACAG AGGAGACTCG ACTGAAGCTG CTGAAGAATC TCCTCAGTAA ACACAGAAAT 
    CCTCTGTCTC AGAAAGAGCT CAGTCAGCTG GCCAGGCTGA CCGACGGTTA CTCTGGCAGC 
    GATCTCACCT CTCTGGCTAA AGACGCTGCT CTCGGCCCGA TACGAGAGCT GAAGCCGGAG 
    CAGGTGAGGA ACATGTCAGC CCATGAGATG CGTGACATTC GCATCTCTGA TTTCCTGGAG 
    TCTCTGAAGC GGATCAAGCG GAGCGTCAGT CCGCAGACCT TGGATCAGTA CGTGCGCTGG 
    AACCGCGAGT ACGGAGACAC GACTGGCGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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