topbp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	topbp1
Entrez Gene ID	407679
Full Name	DNA topoisomerase II binding protein 1
General protein information	Preferred Names DNA topoisomerase II binding protein 1 Names DNA topoisomerase 2-binding protein 1 topoisomerase (DNA) II binding protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297292.2	XP_009295567.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_005162567.2	XP_005162624.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_017353819.1	XP_017209308.1	DNA topoisomerase 2-binding protein 1 isoform X2
XM_005162567.3	XP_005162624.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_009297292.3	XP_009295567.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_017353819.2	XP_017209308.1	DNA topoisomerase 2-binding protein 1 isoform X2
NM_001199738.1	NP_001186667.1	DNA topoisomerase 2-binding protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TOPBP1	NP_001193695.1
Rattus norvegicus (Norway rat)	Topbp1	XP_236578.6
Homo sapiens (human)	TOPBP1	NP_008958.2
Danio rerio (zebrafish)	topbp1	NP_001186667.1
Xenopus tropicalis (tropical clawed frog)	topbp1	XP_004915426.1
	TOPBP1	XP_001112636.2
Mus musculus (house mouse)	Topbp1	NP_795953.2
Gallus gallus (chicken)	TOPBP1	XP_418794.1
Pan troglodytes (chimpanzee)	TOPBP1	XP_516761.3
Canis lupus familiaris (dog)	TOPBP1	XP_005634503.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis.
Gao L, Li D, Ma K, Zhang W, Xu T, Fu C, Jing C, Jia X, Wu S, Sun X, Dong M, Deng M, Chen Y, Zhu W, Peng J, Wan F, Zhou Y, Zon LI, Pan W
PLoS genetics11(7)e1005346(2015 Jul)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa57587	XM_017353819.2 Latest version!	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa28353	NM_001199738.1 Latest version!	Danio rerio DNA topoisomerase II binding protein 1 (topbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa28354	XM_009297292.3 Latest version!	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa28354	XM_005162567.3 Latest version!	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa28354	XM_005162567.2	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa28354	XM_009297292.2	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa57587	XM_017353819.1	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

topbp1 ( XM_017353819.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209308.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57587
Clone ID Related Accession (Same CDS sequence)	XM_017353819.1 , XM_017353819.2
Accession Version	XM_017353819.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG GGAGCGTTTG GCGGCTGGAT TGTGATGCTG 
AATATTGACC AGGCCAGAGA GGCTGGATTC AGAAGACTGC TGCAGTCTGG AGGAGCCAAG 
GTGCTGCCAG ATCCTTCTCC CTCACTGTTT AAAGAGACCA CTCACCTGTT TGTGGATTTC 
AGTCGGTTAA AACCAGGTGA TGTTAGAGTG GACATCAGTG AGGCTTCAGC TCACGGGGTC 
AAATGTCTTA AACCAGAGTA CATTGCTGAT TATCTCATGC AGGAACCGTC TCCTTCAATG 
GACGCTTACT ATCTTCCTGG AGCAGCTGCC GATGAGACAG AGAAGGTTGT AGATGCATCT 
TCACGCAAAC GGAAAGCCTC TGGGGACATG TCCACACTGA AGAAAAGCCG TATGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209308.1
CDS	227..4786
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_017353819.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353819.1

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG GGAGCGTTTG GCGGCTGGAT TGTGATGCTG 
AATATTGACC AGGCCAGAGA GGCTGGATTC AGAAGACTGC TGCAGTCTGG AGGAGCCAAG 
GTGCTGCCAG ATCCTTCTCC CTCACTGTTT AAAGAGACCA CTCACCTGTT TGTGGATTTC 
AGTCGGTTAA AACCAGGTGA TGTTAGAGTG GACATCAGTG AGGCTTCAGC TCACGGGGTC 
AAATGTCTTA AACCAGAGTA CATTGCTGAT TATCTCATGC AGGAACCGTC TCCTTCAATG 
GACGCTTACT ATCTTCCTGG AGCAGCTGCC GATGAGACAG AGAAGGTTGT AGATGCATCT 
TCACGCAAAC GGAAAGCCTC TGGGGACATG TCCACACTGA AGAAAAGCCG TATGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_017353819.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209308.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

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CloneID	ODa57587
Clone ID Related Accession (Same CDS sequence)	XM_017353819.1 , XM_017353819.2
Accession Version	XM_017353819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG GGAGCGTTTG GCGGCTGGAT TGTGATGCTG 
AATATTGACC AGGCCAGAGA GGCTGGATTC AGAAGACTGC TGCAGTCTGG AGGAGCCAAG 
GTGCTGCCAG ATCCTTCTCC CTCACTGTTT AAAGAGACCA CTCACCTGTT TGTGGATTTC 
AGTCGGTTAA AACCAGGTGA TGTTAGAGTG GACATCAGTG AGGCTTCAGC TCACGGGGTC 
AAATGTCTTA AACCAGAGTA CATTGCTGAT TATCTCATGC AGGAACCGTC TCCTTCAATG 
GACGCTTACT ATCTTCCTGG AGCAGCTGCC GATGAGACAG AGAAGGTTGT AGATGCATCT 
TCACGCAAAC GGAAAGCCTC TGGGGACATG TCCACACTGA AGAAAAGCCG TATGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209308.1
CDS	256..4815
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_017353819.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353819.2

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG GGAGCGTTTG GCGGCTGGAT TGTGATGCTG 
AATATTGACC AGGCCAGAGA GGCTGGATTC AGAAGACTGC TGCAGTCTGG AGGAGCCAAG 
GTGCTGCCAG ATCCTTCTCC CTCACTGTTT AAAGAGACCA CTCACCTGTT TGTGGATTTC 
AGTCGGTTAA AACCAGGTGA TGTTAGAGTG GACATCAGTG AGGCTTCAGC TCACGGGGTC 
AAATGTCTTA AACCAGAGTA CATTGCTGAT TATCTCATGC AGGAACCGTC TCCTTCAATG 
GACGCTTACT ATCTTCCTGG AGCAGCTGCC GATGAGACAG AGAAGGTTGT AGATGCATCT 
TCACGCAAAC GGAAAGCCTC TGGGGACATG TCCACACTGA AGAAAAGCCG TATGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( NM_001199738.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186667.1 Danio rerio DNA topoisomerase II binding protein 1 (topbp1), mRNA.

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CloneID	ODa28353
Clone ID Related Accession (Same CDS sequence)	NM_001199738.1
Accession Version	NM_001199738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649325.3. On Dec 17, 2010 this sequence version replaced XR_029693.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG CCTAATGTTA TTGTTGTGCA GGGAGCGTTT 
GGCGGCTGGA TTGTGATGCT GAATATTGAC CAGGCCAGAG AGGCTGGATT CAGAAGACTG 
CTGCAGTCTG GAGGAGCCAA GGTGCTGCCA GATCCTTCTC CCTCACTGTT TAAAGAGACC 
ACTCACCTGT TTGTGGATTT CAGTCGGTTA AAACCAGGTG ATGTTAGAGT GGACATCAGT 
GAGGCTTCAG CTCACGGGGT CAAATGTCTT AAACCAGAGT ACATTGCTGA TTATCTCATG 
CAGGAACCGT CTCCTTCAAT GGACGCTTAC TATCTTCCTG GAGCAGCTGC CGATGAGACA 
GAGAAGGTTG TAGATGCATC TTCACGCAAA CGGAAAGCCT CTGGGGACAT GTCCACACTG 
AAGAAAAGCC GTATGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186667.1
CDS	1..4581
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEPNVIVVQGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	NM_001199738.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA topoisomerase II binding protein 1 (topbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199738.1

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGAGCT GCCTCAGGAC ACCAGCAGTA GGCATAGCGT TTTACATCCA 
GAACCTCTGG TTGAGGGGAA GGACTCCACG TTGGCTGAAG CCTCAGAAGG TGGCCTTTTT 
TTTGGGAAGC GCTTCATCTT GGTTGGTTTC GGAGCTGAGG CTGAAGCCCA GCTGTCAGAG 
CTAGTCATGG AAAACGCTGG GAAGATTTTG GTTGGCAGAT CAAGAGCTGT GGCGCATTAT 
GCTATCGTGC CGCTGCTGGG CTGTGACGTG GAGGCAACAG TAGATGAGGT TGCCACAGAT 
ACTTGGCTGG CCATGTGTGT GGAGCAGCAG TGTGTGTTGC CACTTGCCTC TCATCCTCTG 
TTCACTCCAG TGGCGGTGAG AGAAGGCTTC TCTCCTCTTA AAGACTGTGT GCTGTCAGTC 
AGTCAGTTCA CTGGAGCTGA GAGAGATTCA CTTATTCAGC TCGCCAAACA CCTGGGAGCG 
AGTGTCCAGG ATTACTTTGT GCGGACGGCT AATCAGAGGA AGGGAATGCT GGCCAGCACA 
CATTTGGTTC TCCAGACACC TGAGGGCACC AAGTACCAGG CAGCTCAGAA GTGGGGTCTG 
CCTGCGGTCA CGATTCGCTG GGTTTTAGAG TCTGCTAGAA CAGGGAAACG TGCGGATGAG 
GGCCAATACC TGGTTGACCT GCCACCTTCT CCAGAGAGAA CAGAAGACAG TTTTGTTGGA 
GCATCTCAGA AAGATCCTCC ACCACTGCGC CTCCATCAGC GCTCACCAGA ACTTCCCTTA 
CTGGGCCCAC AAAATAGCGA TGCCATCACC CCTCTAGACA CGGCTCGCTT TCAGAGCCGT 
AGTGTCCGCT CGGCAGTCAG GAAGCTGAAA CAGGGAGAGG AAGAGCAGGC TGAGGTGACC 
ACCCCAGGAC AGGAGGAGGC CAAAGGAGCA CAGCAGAAAG ACTCATCTCT CCATCTAGAT 
ACTCCCTCTC GCTTCCTAAG CAGAGACAAA CTCTTCGGAC CGTCATTTAA CACAAAGGAT 
GTGTTTGACC ATTTACAGAC TCCTGGTAAT AAATCCCAGC AAGGTCAGGG GGCGGAGACT 
CCTCTGTCAG AGGTCATCGA GAGGAACCTT AAAGCTGCCG TTGCCAACAG CAACCGCAGT 
CACGTCACAA ACCTCGCAGC CATGACTGCC AGTCCACAAC TGGGTAAAGA GCCTGAACCT 
GAGGTGGAGC AGAAGGCTGC AGCTCCTCTC AGTGGGGTGG TGGTGTGTGT GAGCAAGAAA 
TTAAGCAAGA AACAGAGTGA ACTCAATGCA GTGGCTGCTT CTCTCGGTGC TGAGTTTAGA 
TGGTCTTGTG ACGACTCGGT CACTCATTAC ATCTACCAGG GGAAAGTGGG AGACAACAGT 
AAAGAATACA GAGCTGTCAA AGAGAGAGGA CTCTATATAG TTTCACAACA CTGGCTACAG 
GCTTGTGCAG ATGAGCAGAA GCATGTGTCG GAGTCGCTCT ACCCTTTTAC ATTCAACCCT 
AAAATGAGCC TCACCATGAG TCAGATGGCA GATTCCACTC AGAGATCACC TCCTTCTGCC 
ACCCCCCGCA CCAGACTGAG AGCTTTCGCT GAGAATGAGG AGACCAGGTT GGGCCCTGAT 
GATGACATCA CCGACGTCTC AACTCCTAAG CAAGGCAGTG TGGGAGAGCC TGAGAAAGAT 
CAAACCATCA GCACCGAGAA GAAAAATCTC ACGGAAACAC TGGAGATGAG GGAAAGTCTT 
CAAAGGCAGC TTCAGGAGAT CATGTCTGCC ACTAAACTAA CCAGCGGTCG TCGAGGCTCC 
TCGCGGTTGG CCAAAACAGG CCCAGGGGGG CTGGACTCTC CCCACACCCC GGACAGCCTG 
GGGCGGATGA GCAGACGCAG TCGCAATTTA GAGGCCATAC GGATGTCACG TCAAGTATGT 
GTGGATCTGA ACACAGAGCC ATCCCAGAGT GAGCAGATCG TTTGGGATGA CCCCACAGCA 
CGAGAAGAGA GAGCTAAACT GGCTGATAAC CTGCAGTGGC CTGGCAGCCC CTCTCAGCAC 
TCTGAACCTC TGGCGCTCGT CCCACCGCCA CACTCTGACC ACACAACCAA CGGCAGAGAC 
TCCATGACTG ACTCTGAACT GGTGGAAATG GCTGCGTTTG AAGTCATCGA TGCACAGCTA 
AAGCCAAAAG GAACCTCGTC TCGAGTCGAC ACACTACACA ACCCTAAAAC ACCTGAAGCA 
CCGAGTATTG CTTTCCCCAT CTCTAAGACT GCAGCCCTCC CTCTTCCACG GATTGAAGAT 
GAGGAGGAAG AGCTGAAGAA GGATCCTCCA CGGTTTTTGC TTTCATCATT GAATCCACAG 
GAACGCATCG ACTACAGCCA TCTCATAGAG GAACTTGGCG GTGTTGTGTT GGAGAAGCAG 
AGTTTTGACC CAAGCTGCAC TCATGTGATT GTGGGTCACC CTCTGAGGAA TGAGAAGTAT 
CTGGCCGCTA TGGCCGCGGG CAAGTGGATC CTTCACCGCT CATACCTGGA GGCCTGCCGA 
GCAGAAGGAC ACTTCATACA GGAGGATCAG TACGAGTGGG GAAGCAGCTC TATACTGGAG 
GCTTTGCCAT CCATTAACTC TCAGCAGAAG AGACTGGCAC TGGCCGCCAT GCGATGGAGG 
AAAACACTAC AAGGCTGCTC AAACAAGGAG CCTAATGTTA TTGTTGTGCA GGGAGCGTTT 
GGCGGCTGGA TTGTGATGCT GAATATTGAC CAGGCCAGAG AGGCTGGATT CAGAAGACTG 
CTGCAGTCTG GAGGAGCCAA GGTGCTGCCA GATCCTTCTC CCTCACTGTT TAAAGAGACC 
ACTCACCTGT TTGTGGATTT CAGTCGGTTA AAACCAGGTG ATGTTAGAGT GGACATCAGT 
GAGGCTTCAG CTCACGGGGT CAAATGTCTT AAACCAGAGT ACATTGCTGA TTATCTCATG 
CAGGAACCGT CTCCTTCAAT GGACGCTTAC TATCTTCCTG GAGCAGCTGC CGATGAGACA 
GAGAAGGTTG TAGATGCATC TTCACGCAAA CGGAAAGCCT CTGGGGACAT GTCCACACTG 
AAGAAAAGCC GTATGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_009297292.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295567.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

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CloneID	ODa28354
Clone ID Related Accession (Same CDS sequence)	XM_009297292.2 , XM_005162567.2 , XM_005162567.3 , XM_009297292.3
Accession Version	XM_009297292.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295567.1
CDS	216..4778
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_009297292.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297292.2

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_005162567.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162624.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

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CloneID	ODa28354
Clone ID Related Accession (Same CDS sequence)	XM_009297292.2 , XM_005162567.2 , XM_005162567.3 , XM_009297292.3
Accession Version	XM_005162567.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162624.1
CDS	227..4789
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_005162567.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162567.2

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_005162567.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162624.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28354
Clone ID Related Accession (Same CDS sequence)	XM_009297292.2 , XM_005162567.2 , XM_005162567.3 , XM_009297292.3
Accession Version	XM_005162567.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162624.1
CDS	256..4818
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_005162567.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162567.3

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_009297292.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295567.1 Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28354
Clone ID Related Accession (Same CDS sequence)	XM_009297292.2 , XM_005162567.2 , XM_005162567.3 , XM_009297292.3
Accession Version	XM_009297292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295567.1
CDS	245..4807
Translation	MSKASKDAYIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERGDKRKTDDKSGRPNTSTPTGQSRSKEDGPSLLGLSHISNISLNVNETALTTAGISHIEMPDPVDSFDITVCRVDDLLDGCKLYLCGLSGKKLEKLRRMVNTAGGLRFNQPTQELTHIVMGEADQGIKAFLDNATHRPHIVTVQWLLESFSRGSLLPENGYFHPSFLPPAPAAVDIPALHKAAPRASRPSVAPPPAPSPARQSRAEEDLLSQYMGNDQTVVELPQDTSSRHSVLHPEPLVEGKDSTLAEASEGGLFFGKRFILVGFGAEAEAQLSELVMENAGKILVGRSRAVAHYAIVPLLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSPLKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLVDLPPSPERTEDSFVGASQKDPPPLRLHQRSPELPLLGPQNSDAITPLDTARFQSRSVRSAVRKLKQGEEEQAEVTTPGQEEAKGAQQKDSSLHLDTPSRFLSRDKLFGPSFNTKDVFDHLQTPGNKSQQGQGAETPLSEVIERNLKAAVANSNRSHVTNLAAMTASPQLGKEPEPEVEQKAAAPLSGVVVCVSKKLSKKQSELNAVAASLGAEFRWSCDDSVTHYIYQGKVGDNSKEYRAVKERGLYIVSQHWLQACADEQKHVSESLYPFTFNPKMSLTMSQMADSTQRSPPSATPRTRLRAFAENEETRLGPDDDITDVSTPKQGSVGEPEKDQTISTEKKNLTETLEMRESLQRQLQEIMSATKLTSGRRGSSRLAKTGPGGLDSPHTPDSLGRMSRRSRNLEAIRMSRQVCVDLNTEPSQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLALVPPPHSDHTTNGRDSMTDSELVEMAAFEVIDAQLKPKGTSSRVDTLHNPKTPEAPSIAFPISKTAALPLPRIEDEEEELKKDPPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVDFSRLKPGDVRVDISEASAHGVKCLKPEYIADYLMQEPSPSMDAYYLPGAAADETEKVVDASSRKRKASGDMSTLKKSRMR

Target ORF information:

RefSeq Version	XM_009297292.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297292.3

ORF Insert Sequence:

ATGTCGAAAG CCAGCAAGGA TGCATACATT GTGAAGTTTG TTGAAAGCAA TGGACAAAAG 
AGTGATGTGG CTCTCCAGGC CTATAAGGCG ATAGTGGAAT TGCAGTCAGA AAAATATGTG 
CAGATGGTGG ATGAGGAAGC GGCTCTGGAG CTGGACCACA ATGACAAATC CCTGTTTGTG 
TTCAGTGACT TCACCAGTAG TGCCTTTGAC CACTGCAGAA GGCTGGGATG CAGGATTGTG 
AGTCCTCTAG TAGTGCTCTT CTGTCTGCAG AAGCAGAGAT GTGTGCCCAA AGCAGAGCAG 
CCCGTGTATA ACATGTCCAT GGCTGATGTC ACTGTTTCCT GCACTAACCT TGACAAAGAG 
GCACGGAGTG AAGTGATGGA TCTTATTCAG CTGATGGGTG GACGAGTCTA CCGGGATCTC 
AACGTGTCTG TTACTCACCT GGTGGCAGGT GAAGTTGGGA GTAAAAAATA TCTGGTGGCA 
GCAAGCCTTG GGAAGCCCAT CCTGTTACCC AGCTGGGTGA AAGCATGCTG GGAAAAGTCT 
CAGGACAGTG TTTTCCATCA CTCTGAGCTG AATACAGAGG ATTATCGGTG TCCAGTCCTT 
AAAGGCTGTA CAGTGTGTGT GACGGGCCTC TCTACTGTTG AACGTAAGGA GGTTCAGAGG 
TTGTGTGATC AGAATGGAGC CACCTACACA GGGCAGCTCA AGATGAATGA GTGCACTCAC 
CTTATTGTCA ATGAACCCAC AGGTCAGAAA TATGAGTTTG CCCGTAAATG GAACGTGTAC 
TGCGTGTCAC TCCACTGGCT GTTTGACAGC ATAGAGAAGG GCTTCTGTCA GGATGAGAGC 
AGGTATGCTG TGGAGCGTGG AGATAAGAGA AAAACTGATG ATAAGTCTGG CAGACCAAAC 
ACTTCTACTC CTACAGGACA GAGCAGGAGT AAAGAGGATG GTCCCTCGCT TTTGGGATTG 
AGTCATATCT CCAACATCAG CCTTAATGTC AATGAAACAG CCCTCACTAC AGCAGGGATC 
AGCCACATCG AGATGCCTGA TCCAGTTGAC TCATTTGACA TCACCGTCTG TCGAGTTGAT 
GACTTGCTGG ATGGCTGTAA GTTGTATTTG TGTGGCCTGT CAGGCAAGAA ACTGGAGAAA 
TTGAGGCGAA TGGTGAACAC TGCTGGAGGT CTGCGCTTTA ACCAGCCCAC CCAAGAACTT 
ACACATATAG TAATGGGAGA AGCAGACCAG GGAATCAAAG CTTTCCTTGA CAACGCAACT 
CACAGGCCTC ATATAGTGAC CGTGCAGTGG CTTCTAGAAA GTTTTTCCCG TGGTTCCTTG 
CTCCCCGAGA ATGGCTACTT CCACCCGTCT TTCCTGCCCC CAGCCCCAGC CGCTGTTGAT 
ATACCTGCTC TTCACAAAGC TGCCCCTCGT GCCTCCAGAC CCTCTGTAGC TCCCCCTCCT 
GCCCCTAGTC CAGCCCGACA GTCCAGAGCA GAGGAAGACC TGCTCTCCCA GTACATGGGG 
AACGACCAGA CTGTGGTAGA GCTGCCTCAG GACACCAGCA GTAGGCATAG CGTTTTACAT 
CCAGAACCTC TGGTTGAGGG GAAGGACTCC ACGTTGGCTG AAGCCTCAGA AGGTGGCCTT 
TTTTTTGGGA AGCGCTTCAT CTTGGTTGGT TTCGGAGCTG AGGCTGAAGC CCAGCTGTCA 
GAGCTAGTCA TGGAAAACGC TGGGAAGATT TTGGTTGGCA GATCAAGAGC TGTGGCGCAT 
TATGCTATCG TGCCGCTGCT GGGCTGTGAC GTGGAGGCAA CAGTAGATGA GGTTGCCACA 
GATACTTGGC TGGCCATGTG TGTGGAGCAG CAGTGTGTGT TGCCACTTGC CTCTCATCCT 
CTGTTCACTC CAGTGGCGGT GAGAGAAGGC TTCTCTCCTC TTAAAGACTG TGTGCTGTCA 
GTCAGTCAGT TCACTGGAGC TGAGAGAGAT TCACTTATTC AGCTCGCCAA ACACCTGGGA 
GCGAGTGTCC AGGATTACTT TGTGCGGACG GCTAATCAGA GGAAGGGAAT GCTGGCCAGC 
ACACATTTGG TTCTCCAGAC ACCTGAGGGC ACCAAGTACC AGGCAGCTCA GAAGTGGGGT 
CTGCCTGCGG TCACGATTCG CTGGGTTTTA GAGTCTGCTA GAACAGGGAA ACGTGCGGAT 
GAGGGCCAAT ACCTGGTTGA CCTGCCACCT TCTCCAGAGA GAACAGAAGA CAGTTTTGTT 
GGAGCATCTC AGAAAGATCC TCCACCACTG CGCCTCCATC AGCGCTCACC AGAACTTCCC 
TTACTGGGCC CACAAAATAG CGATGCCATC ACCCCTCTAG ACACGGCTCG CTTTCAGAGC 
CGTAGTGTCC GCTCGGCAGT CAGGAAGCTG AAACAGGGAG AGGAAGAGCA GGCTGAGGTG 
ACCACCCCAG GACAGGAGGA GGCCAAAGGA GCACAGCAGA AAGACTCATC TCTCCATCTA 
GATACTCCCT CTCGCTTCCT AAGCAGAGAC AAACTCTTCG GACCGTCATT TAACACAAAG 
GATGTGTTTG ACCATTTACA GACTCCTGGT AATAAATCCC AGCAAGGTCA GGGGGCGGAG 
ACTCCTCTGT CAGAGGTCAT CGAGAGGAAC CTTAAAGCTG CCGTTGCCAA CAGCAACCGC 
AGTCACGTCA CAAACCTCGC AGCCATGACT GCCAGTCCAC AACTGGGTAA AGAGCCTGAA 
CCTGAGGTGG AGCAGAAGGC TGCAGCTCCT CTCAGTGGGG TGGTGGTGTG TGTGAGCAAG 
AAATTAAGCA AGAAACAGAG TGAACTCAAT GCAGTGGCTG CTTCTCTCGG TGCTGAGTTT 
AGATGGTCTT GTGACGACTC GGTCACTCAT TACATCTACC AGGGGAAAGT GGGAGACAAC 
AGTAAAGAAT ACAGAGCTGT CAAAGAGAGA GGACTCTATA TAGTTTCACA ACACTGGCTA 
CAGGCTTGTG CAGATGAGCA GAAGCATGTG TCGGAGTCGC TCTACCCTTT TACATTCAAC 
CCTAAAATGA GCCTCACCAT GAGTCAGATG GCAGATTCCA CTCAGAGATC ACCTCCTTCT 
GCCACCCCCC GCACCAGACT GAGAGCTTTC GCTGAGAATG AGGAGACCAG GTTGGGCCCT 
GATGATGACA TCACCGACGT CTCAACTCCT AAGCAAGGCA GTGTGGGAGA GCCTGAGAAA 
GATCAAACCA TCAGCACCGA GAAGAAAAAT CTCACGGAAA CACTGGAGAT GAGGGAAAGT 
CTTCAAAGGC AGCTTCAGGA GATCATGTCT GCCACTAAAC TAACCAGCGG TCGTCGAGGC 
TCCTCGCGGT TGGCCAAAAC AGGCCCAGGG GGGCTGGACT CTCCCCACAC CCCGGACAGC 
CTGGGGCGGA TGAGCAGACG CAGTCGCAAT TTAGAGGCCA TACGGATGTC ACGTCAAGTA 
TGTGTGGATC TGAACACAGA GCCATCCCAG AGTGAGCAGA TCGTTTGGGA TGACCCCACA 
GCACGAGAAG AGAGAGCTAA ACTGGCTGAT AACCTGCAGT GGCCTGGCAG CCCCTCTCAG 
CACTCTGAAC CTCTGGCGCT CGTCCCACCG CCACACTCTG ACCACACAAC CAACGGCAGA 
GACTCCATGA CTGACTCTGA ACTGGTGGAA ATGGCTGCGT TTGAAGTCAT CGATGCACAG 
CTAAAGCCAA AAGGAACCTC GTCTCGAGTC GACACACTAC ACAACCCTAA AACACCTGAA 
GCACCGAGTA TTGCTTTCCC CATCTCTAAG ACTGCAGCCC TCCCTCTTCC ACGGATTGAA 
GATGAGGAGG AAGAGCTGAA GAAGGATCCT CCACGGTTTT TGCTTTCATC ATTGAATCCA 
CAGGAACGCA TCGACTACAG CCATCTCATA GAGGAACTTG GCGGTGTTGT GTTGGAGAAG 
CAGAGTTTTG ACCCAAGCTG CACTCATGTG ATTGTGGGTC ACCCTCTGAG GAATGAGAAG 
TATCTGGCCG CTATGGCCGC GGGCAAGTGG ATCCTTCACC GCTCATACCT GGAGGCCTGC 
CGAGCAGAAG GACACTTCAT ACAGGAGGAT CAGTACGAGT GGGGAAGCAG CTCTATACTG 
GAGGCTTTGC CATCCATTAA CTCTCAGCAG AAGAGACTGG CACTGGCCGC CATGCGATGG 
AGGAAAACAC TACAAGGCTG CTCAAACAAG GAGGGAGCGT TTGGCGGCTG GATTGTGATG 
CTGAATATTG ACCAGGCCAG AGAGGCTGGA TTCAGAAGAC TGCTGCAGTC TGGAGGAGCC 
AAGGTGCTGC CAGATCCTTC TCCCTCACTG TTTAAAGAGA CCACTCACCT GTTTGTGGAT 
TTCAGTCGGT TAAAACCAGG TGATGTTAGA GTGGACATCA GTGAGGCTTC AGCTCACGGG 
GTCAAATGTC TTAAACCAGA GTACATTGCT GATTATCTCA TGCAGGAACC GTCTCCTTCA 
ATGGACGCTT ACTATCTTCC TGGAGCAGCT GCCGATGAGA CAGAGAAGGT TGTAGATGCA 
TCTTCACGCA AACGGAAAGC CTCTGGGGAC ATGTCCACAC TGAAGAAAAG CCGTATGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis.
PLoS genetics11(7)e1005346(2015 Jul)
Gao L,Li D,Ma K,Zhang W,Xu T,Fu C,Jing C,Jia X,Wu S,Sun X,Dong M,Deng M,Chen Y,Zhu W,Peng J,Wan F,Zhou Y,Zon LI,Pan W

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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topbp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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