acana cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	acana
Entrez Gene ID	497505
Full Name	aggrecan a
Synonyms	acan,agc1,im:7146049
General protein information	Preferred Names aggrecan a Names aggrecan a aggrecan core protein agca aggrecan 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_681090.9	XP_686182.6	aggrecan core protein isoform X3
XM_005166485.3	XP_005166542.1	aggrecan core protein isoform X1
XM_681090.8	XP_686182.6	aggrecan core protein isoform X2
XM_005166485.4	XP_005166542.1	aggrecan core protein isoform X1
XM_021477951.1	XP_021333626.1	aggrecan core protein isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acana	XP_005166542.1
Danio rerio (zebrafish)	acanb	XP_688042.4
Homo sapiens (human)	ACAN	NP_001126.3
Xenopus tropicalis (tropical clawed frog)	acan	XP_002932297.2

Gene Ontology

The following acana gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acana cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13397	XM_005166485.4 Latest version!	Danio rerio aggrecan a (acana), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa13398	XM_681090.9 Latest version!	Danio rerio aggrecan a (acana), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa143513	XM_021477951.1 Latest version!	Danio rerio aggrecan a (acana), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
ODa13398	XM_681090.8	Danio rerio aggrecan a (acana), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa13397	XM_005166485.3	Danio rerio aggrecan a (acana), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acana ( XM_005166485.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166542.1 Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13397
Clone ID Related Accession (Same CDS sequence)	XM_005166485.3 , XM_005166485.4
Accession Version	XM_005166485.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	aggrecan core protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT 
TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA 
TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC 
TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT 
GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG 
CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA 
GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA 
AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA 
AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT 
CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA 
GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC 
CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC 
ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA 
ACAGCATTGG AGAAACAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166542.1
CDS	157..4800
Translation	MLSLLLLSMCLWVFSATASFDYIDTEPESPLRVSIPNELPLRPLMGDTLVLPCYFQDNTVNDPGAPTIAPLSHRIKWSLVTKEKTTNILVASEGIVSIEKRYMDRITLVGYPMTPTDASIKITELHSNDSGVYRCEVMQGIEDSHDIVDVQVQGIVFHYRAITTRYTLNFEKAKAACIQNSAVIATPEQLQAAYDEGFHQCDAGWLADQTVRYPIHDPREACYGDKYEFPGVRTYGVRDVNETYDVYCFAERMTGKVFHTTSPNKFTFEEAEAQCSKLGAKLATTGQLYLAWKGGMDVCNAGWLADRSVRYPINIARPQCGGGLLGVRTVYLFPNQTGYPHPDSRYDAFCYSETGDEGSGFFPLDLELTTDGDNGVLTVDTITESHTVISKPASTESEVQGEVVTYKPSVTTSVENPYTALDISHVLPSPPTSSVEKDPDFGSGLSQSNKTEDTMSATGVVFHYRGGNCSRYPFGFVEAQLACKSIGAVIASAQQLQAAYEAGYHQCDAGWLSDQTVRYPIVSPRHQCSGDMEQVPGVRSYGLRPADERYDVYCYVDKLRGQVFHASSFEGFTYGEAVAHCQGQNASLASTAHLYAAWKQGFDKCRAGWLLDRSVRYSINIPRQQCGGGRTGVHTVYLFPNQTGSPDLHSKFDAYCFKEDLDLPLNESESQETVTEEKIVSISSHPALHRPEGTPIIAPSPTEPSGTEESVASSSGDLLSSGSGDQPSGSSVSSGDSSGTSSTDDLLTSGSGDQSGSGSSVTSGDYSGASGTDSLFSSGSGDRSSGSFSSSGDFSGASGTDDLLGSGSGEQPSGSFVSSGDFSGASGSGDMSSGSGSSSGSGSGLDILVTFSGIDTVFSGVGSTSGQLEEAGEGSTEILTFQLDQISGSGSGLGSGSGYSSEESGSTSDLSSSSGESGESGDLSGISSGSGFSEESSGFSGFASGIFPSGGSSDISFIDGSGIIMVDRPWMKISSFLTEQELGSGQVDFSGSGLFSGSGVSGDISGLSGEISGSSGDSSGISSDISGMSGDISGMSGDISGISGDITGMSGDLSGMSSSDHSGSGSGLAFSGVTFLGSGFTDLTEQSREQEASGLVFFGSGEGSGFRSGGYAIESGERSGDSASSDVIFFSNNDMVEMSNKPLQGMELGRGEVEYSGTVRLSSGSGDDQSVFISGHEWIPVTANTPSVKSTDPPPAELFDQGPSDLYSDTAGHLGPYLKPEEIINNLDAVPLAALSSTTETASLQRPAVVERPAVNDYLNPCEPNPCGAGVCSVKDGVGLCHCPPGLHGEECQFEVDVCHSNPCANGATCVEVEDTFKCLCLPSYEGDRCETDSHSCEKGWTKFQGNCYLHFSKRETWLDAEQRCRDLNAHLVSINTPEEQAFVNSNAQDYQWIGLNDKTVENDFRWSDGTQLQFENWRPNQPDNYFNSEEDCVVMIWHENGQWNDVPCNYHLPFTCKSGPVTCDKPPKVENAKMFGNKKERYQVNSIIRYQCSENFTQRHPPVIRCMADGQWEKPKVQCIPKVKFGLHDKSVHHSHKARTALEKQL

Target ORF information:

RefSeq Version	XM_005166485.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166485.3

ORF Insert Sequence:

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT 
TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA 
TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC 
TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT 
GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG 
CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA 
GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA 
AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA 
AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT 
CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA 
GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC 
CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC 
ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA 
ACAGCATTGG AGAAACAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acana ( XM_005166485.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166542.1 Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13397
Clone ID Related Accession (Same CDS sequence)	XM_005166485.3 , XM_005166485.4
Accession Version	XM_005166485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT 
TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA 
TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC 
TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT 
GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG 
CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA 
GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA 
AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA 
AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT 
CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA 
GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC 
CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC 
ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA 
ACAGCATTGG AGAAACAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166542.1
CDS	167..4810
Translation	MLSLLLLSMCLWVFSATASFDYIDTEPESPLRVSIPNELPLRPLMGDTLVLPCYFQDNTVNDPGAPTIAPLSHRIKWSLVTKEKTTNILVASEGIVSIEKRYMDRITLVGYPMTPTDASIKITELHSNDSGVYRCEVMQGIEDSHDIVDVQVQGIVFHYRAITTRYTLNFEKAKAACIQNSAVIATPEQLQAAYDEGFHQCDAGWLADQTVRYPIHDPREACYGDKYEFPGVRTYGVRDVNETYDVYCFAERMTGKVFHTTSPNKFTFEEAEAQCSKLGAKLATTGQLYLAWKGGMDVCNAGWLADRSVRYPINIARPQCGGGLLGVRTVYLFPNQTGYPHPDSRYDAFCYSETGDEGSGFFPLDLELTTDGDNGVLTVDTITESHTVISKPASTESEVQGEVVTYKPSVTTSVENPYTALDISHVLPSPPTSSVEKDPDFGSGLSQSNKTEDTMSATGVVFHYRGGNCSRYPFGFVEAQLACKSIGAVIASAQQLQAAYEAGYHQCDAGWLSDQTVRYPIVSPRHQCSGDMEQVPGVRSYGLRPADERYDVYCYVDKLRGQVFHASSFEGFTYGEAVAHCQGQNASLASTAHLYAAWKQGFDKCRAGWLLDRSVRYSINIPRQQCGGGRTGVHTVYLFPNQTGSPDLHSKFDAYCFKEDLDLPLNESESQETVTEEKIVSISSHPALHRPEGTPIIAPSPTEPSGTEESVASSSGDLLSSGSGDQPSGSSVSSGDSSGTSSTDDLLTSGSGDQSGSGSSVTSGDYSGASGTDSLFSSGSGDRSSGSFSSSGDFSGASGTDDLLGSGSGEQPSGSFVSSGDFSGASGSGDMSSGSGSSSGSGSGLDILVTFSGIDTVFSGVGSTSGQLEEAGEGSTEILTFQLDQISGSGSGLGSGSGYSSEESGSTSDLSSSSGESGESGDLSGISSGSGFSEESSGFSGFASGIFPSGGSSDISFIDGSGIIMVDRPWMKISSFLTEQELGSGQVDFSGSGLFSGSGVSGDISGLSGEISGSSGDSSGISSDISGMSGDISGMSGDISGISGDITGMSGDLSGMSSSDHSGSGSGLAFSGVTFLGSGFTDLTEQSREQEASGLVFFGSGEGSGFRSGGYAIESGERSGDSASSDVIFFSNNDMVEMSNKPLQGMELGRGEVEYSGTVRLSSGSGDDQSVFISGHEWIPVTANTPSVKSTDPPPAELFDQGPSDLYSDTAGHLGPYLKPEEIINNLDAVPLAALSSTTETASLQRPAVVERPAVNDYLNPCEPNPCGAGVCSVKDGVGLCHCPPGLHGEECQFEVDVCHSNPCANGATCVEVEDTFKCLCLPSYEGDRCETDSHSCEKGWTKFQGNCYLHFSKRETWLDAEQRCRDLNAHLVSINTPEEQAFVNSNAQDYQWIGLNDKTVENDFRWSDGTQLQFENWRPNQPDNYFNSEEDCVVMIWHENGQWNDVPCNYHLPFTCKSGPVTCDKPPKVENAKMFGNKKERYQVNSIIRYQCSENFTQRHPPVIRCMADGQWEKPKVQCIPKVKFGLHDKSVHHSHKARTALEKQL

Target ORF information:

RefSeq Version	XM_005166485.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166485.4

ORF Insert Sequence:

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT 
TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA 
TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC 
TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT 
GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG 
CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA 
GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA 
AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA 
AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT 
CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA 
GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC 
CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC 
ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA 
ACAGCATTGG AGAAACAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acana ( XM_681090.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686182.6 Danio rerio aggrecan a (acana), transcript variant X3, mRNA.

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CloneID	ODa13398
Clone ID Related Accession (Same CDS sequence)	XM_681090.9 , XM_681090.8
Accession Version	XM_681090.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	aggrecan core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_681090.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG 
AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG 
CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA 
GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT 
AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG 
AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG 
CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA 
TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC 
AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA 
CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT 
CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA 
GCTAGAACAG CATTGGAGAA ACAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686182.6
CDS	168..4697
Translation	MLSLLLLSMCLWVFSATASFDYIDTEPESPLRVSIPNELPLRPLMGDTLVLPCYFQDNTVNDPGAPTIAPLSHRIKWSLVTKEKTTNILVASEGIVSIEKRYMDRITLVGYPMTPTDASIKITELHSNDSGVYRCEVMQGIEDSHDIVDVQVQGIVFHYRAITTRYTLNFEKAKAACIQNSAVIATPEQLQAAYDEGFHQCDAGWLADQTVRYPIHDPREACYGDKYEFPGVRTYGVRDVNETYDVYCFAERMTGKVFHTTSPNKFTFEEAEAQCSKLGAKLATTGQLYLAWKGGMDVCNAGWLADRSVRYPINIARPQCGGGLLGVRTVYLFPNQTGYPHPDSRYDAFCYSETGDEGSGFFPLDLELTTDGDNGVLTVDTITESHTVISKPASTESEVQGEVVTYKPSVTTSVENPYTALDISHVLPSPPTSSVEKDPDFGSGLSQSNKTEDTMSATGVVFHYRGGNCSRYPFGFVEAQLACKSIGAVIASAQQLQAAYEAGYHQCDAGWLSDQTVRYPIVSPRHQCSGDMEQVPGVRSYGLRPADERYDVYCYVDKLRGQVFHASSFEGFTYGEAVAHCQGQNASLASTAHLYAAWKQGFDKCRAGWLLDRSVRYSINIPRQQCGGGRTGVHTVYLFPNQTGSPDLHSKFDAYCFKEDLDLPLNESESQETVTEEKIVSISSHPALHRPEGTPIIAPSPTEPSGTEESVASSSGDLLSSGSGDQPSGSSVSSGDSSGTSSTDDLLTSGSGDQSGSGSSVTSGDYSGASGTDSLFSSGSGDRSSGSFSSSGDFSGASGTDDLLGSGSGEQPSGSFVSSGDFSGASGSGDMSSGSGSSSGSGSGLDILVTFSGIDTVFSGVGSTSGQLEEAGEGSTEILTFQLDQISGSGSGLGSGSGYSSEESGSTSDLSSSSGESGESGDLSGISSGSGFSEESSGFSGFASGIFPSGGSSDISFIDGSGIIMVDRPWMKISSFLTEQELGSGQVDFSGSGLFSGSGVSGDISGLSGEISGSSGDSSGISSDISGMSGDISGMSGDISGISGDITGMSGDLSGMSSSDHSGSGSGLAFSGVTFLGSGFTDLTEQSREQEASGLVFFGSGEGSGFRSGGYAIESGERSGDSASSDVIFFSNNDMVEMSNKPLQGMELGRGEVEYSGTVRLSSGSGDDQSVFISGHEWIPVTANTPSVKSTDPPPAELFDQGPSDLYSDTAGHLGPYLKPEEIINNLDAVPLAALSSTTETASLQRPAVVERPAVNDYLNPCEPNPCGAGVCSVKDGVGLCHCPPGLHGEECQFDSHSCEKGWTKFQGNCYLHFSKRETWLDAEQRCRDLNAHLVSINTPEEQAFVNSNAQDYQWIGLNDKTVENDFRWSDGTQLQFENWRPNQPDNYFNSEEDCVVMIWHENGQWNDVPCNYHLPFTCKSGPVTCDKPPKVENAKMFGNKKERYQVNSIIRYQCSENFTQRHPPVIRCMADGQWEKPKVQCIPKVKFGLHDKSVHHSHKARTALEKQL

Target ORF information:

RefSeq Version	XM_681090.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio aggrecan a (acana), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_681090.9

ORF Insert Sequence:

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG 
AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG 
CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA 
GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT 
AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG 
AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG 
CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA 
TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC 
AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA 
CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT 
CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA 
GCTAGAACAG CATTGGAGAA ACAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acana ( XM_681090.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686182.6 Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

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CloneID	ODa13398
Clone ID Related Accession (Same CDS sequence)	XM_681090.9 , XM_681090.8
Accession Version	XM_681090.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	aggrecan core protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_681090.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG 
AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG 
CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA 
GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT 
AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG 
AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG 
CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA 
TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC 
AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA 
CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT 
CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA 
GCTAGAACAG CATTGGAGAA ACAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686182.6
CDS	157..4686
Translation	MLSLLLLSMCLWVFSATASFDYIDTEPESPLRVSIPNELPLRPLMGDTLVLPCYFQDNTVNDPGAPTIAPLSHRIKWSLVTKEKTTNILVASEGIVSIEKRYMDRITLVGYPMTPTDASIKITELHSNDSGVYRCEVMQGIEDSHDIVDVQVQGIVFHYRAITTRYTLNFEKAKAACIQNSAVIATPEQLQAAYDEGFHQCDAGWLADQTVRYPIHDPREACYGDKYEFPGVRTYGVRDVNETYDVYCFAERMTGKVFHTTSPNKFTFEEAEAQCSKLGAKLATTGQLYLAWKGGMDVCNAGWLADRSVRYPINIARPQCGGGLLGVRTVYLFPNQTGYPHPDSRYDAFCYSETGDEGSGFFPLDLELTTDGDNGVLTVDTITESHTVISKPASTESEVQGEVVTYKPSVTTSVENPYTALDISHVLPSPPTSSVEKDPDFGSGLSQSNKTEDTMSATGVVFHYRGGNCSRYPFGFVEAQLACKSIGAVIASAQQLQAAYEAGYHQCDAGWLSDQTVRYPIVSPRHQCSGDMEQVPGVRSYGLRPADERYDVYCYVDKLRGQVFHASSFEGFTYGEAVAHCQGQNASLASTAHLYAAWKQGFDKCRAGWLLDRSVRYSINIPRQQCGGGRTGVHTVYLFPNQTGSPDLHSKFDAYCFKEDLDLPLNESESQETVTEEKIVSISSHPALHRPEGTPIIAPSPTEPSGTEESVASSSGDLLSSGSGDQPSGSSVSSGDSSGTSSTDDLLTSGSGDQSGSGSSVTSGDYSGASGTDSLFSSGSGDRSSGSFSSSGDFSGASGTDDLLGSGSGEQPSGSFVSSGDFSGASGSGDMSSGSGSSSGSGSGLDILVTFSGIDTVFSGVGSTSGQLEEAGEGSTEILTFQLDQISGSGSGLGSGSGYSSEESGSTSDLSSSSGESGESGDLSGISSGSGFSEESSGFSGFASGIFPSGGSSDISFIDGSGIIMVDRPWMKISSFLTEQELGSGQVDFSGSGLFSGSGVSGDISGLSGEISGSSGDSSGISSDISGMSGDISGMSGDISGISGDITGMSGDLSGMSSSDHSGSGSGLAFSGVTFLGSGFTDLTEQSREQEASGLVFFGSGEGSGFRSGGYAIESGERSGDSASSDVIFFSNNDMVEMSNKPLQGMELGRGEVEYSGTVRLSSGSGDDQSVFISGHEWIPVTANTPSVKSTDPPPAELFDQGPSDLYSDTAGHLGPYLKPEEIINNLDAVPLAALSSTTETASLQRPAVVERPAVNDYLNPCEPNPCGAGVCSVKDGVGLCHCPPGLHGEECQFDSHSCEKGWTKFQGNCYLHFSKRETWLDAEQRCRDLNAHLVSINTPEEQAFVNSNAQDYQWIGLNDKTVENDFRWSDGTQLQFENWRPNQPDNYFNSEEDCVVMIWHENGQWNDVPCNYHLPFTCKSGPVTCDKPPKVENAKMFGNKKERYQVNSIIRYQCSENFTQRHPPVIRCMADGQWEKPKVQCIPKVKFGLHDKSVHHSHKARTALEKQL

Target ORF information:

RefSeq Version	XM_681090.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_681090.8

ORF Insert Sequence:

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT 
CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC 
AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA 
AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT 
GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT 
GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT 
GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA 
GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA 
TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA 
GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA 
TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC 
TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT 
GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT 
GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC 
AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA 
GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG 
AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT 
ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA 
GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC 
CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC 
ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT 
GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG 
GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC 
AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA 
CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA 
GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT 
CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA 
GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA 
ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC 
CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT 
TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG 
AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG 
CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA 
GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT 
AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG 
AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG 
CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA 
TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC 
AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA 
CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT 
CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA 
GCTAGAACAG CATTGGAGAA ACAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acana ( XM_021477951.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333626.1 Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

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CloneID	ODa143513
Clone ID Related Accession (Same CDS sequence)	XM_021477951.1
Accession Version	XM_021477951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGGTACCC CTATCATTGC TCCCAGTCCT 
ACAGAACCTT CTGGAACTGA GGAATCTGTG GCCAGCAGTT CTGGCGATCT GTTGAGCAGT 
GGCAGTGGGG ATCAGCCAAG TGGGTCATCC GTTTCAAGTG GTGACTCCTC CGGGACAAGT 
AGCACTGATG ATCTTTTGAC AAGTGGGAGC GGGGATCAGA GTGGAAGTGG CTCATCTGTT 
ACAAGTGGAG ACTATTCTGG AGCCAGTGGC ACAGACAGTC TGTTCAGTAG TGGCAGTGGC 
GATCGGTCGA GTGGGTCATT CAGTTCCAGT GGAGACTTCT CTGGAGCCAG TGGGACTGAT 
GATTTGTTGG GCAGTGGGAG TGGGGAACAG CCAAGTGGGT CATTTGTGTC AAGCGGAGAC 
TTCTCTGGGG CCAGTGGAAG TGGGGATATG TCAAGTGGTT CTGGAAGCTC TTCAGGATCT 
GGTAGTGGTT TAGATATCTT AGTGACTTTT TCAGGGATTG ACACAGTTTT TTCAGGAGTT 
GGATCAACTT CAGGGCAACT TGAGGAGGCT GGGGAGGGAA GCACTGAGAT TCTTACATTC 
CAATTAGATC AAATCTCTGG ATCTGGATCG GGATTAGGCA GTGGGTCAGG GTACAGCTCT 
GAAGAAAGTG GATCTACCTC AGACCTTTCC AGCAGCTCAG GAGAGAGTGG TGAGAGTGGA 
GATCTTTCTG GCATCTCCTC AGGATCAGGG TTCAGTGAGG AGTCCTCAGG ATTCAGTGGT 
TTTGCATCAG GTATTTTTCC CTCTGGTGGA TCCAGTGACA TTTCATTCAT TGATGGCAGT 
GGAATCATCA TGGTGGACAG GCCGTGGATG AAGATATCTT CATTTTTAAC AGAACAAGAG 
CTGGGTAGTG GTCAAGTCGA TTTCAGTGGA TCAGGACTTT TCTCAGGGTC TGGGGTGAGT 
GGTGATATCT CTGGCTTAAG TGGTGAAATC TCTGGATCGA GTGGTGATAG TTCAGGTATA 
AGTAGTGACA TTTCAGGAAT GAGTGGTGAT ATCTCAGGAA TGAGTGGTGA TATCTCAGGA 
ATAAGTGGTG ATATTACAGG AATGAGTGGT GATCTCTCTG GTATGAGTAG CAGTGACCAC 
AGTGGCTCAG GGAGTGGTTT AGCATTTTCT GGGGTGACAT TTTTGGGGTC AGGCTTCACT 
GATCTCACAG AACAATCTCG TGAACAGGAA GCTTCTGGAC TTGTGTTCTT TGGGTCTGGT 
GAGGGAAGTG GATTTAGATC TGGAGGCTAT GCAATTGAAT CAGGGGAACG ATCAGGGGAC 
TCGGCTAGTA GTGATGTCAT CTTCTTCAGC AATAATGACA TGGTGGAAAT GTCAAACAAG 
CCACTTCAAG GAATGGAATT AGGAAGAGGG GAAGTGGAAT ATAGTGGCAC AGTCAGACTG 
TCCTCAGGGA GTGGAGATGA CCAAAGTGTC TTTATAAGCG GGCATGAATG GATCCCAGTC 
ACTGCTAATA CACCATCTGT AAAATCCACT GATCCTCCCC CAGCTGAATT ATTTGATCAA 
GGTCCATCAG ACCTGTACAG TGATACTGCA GGACACCTAG GTCCTTATTT AAAACCAGAA 
GAGATCATCA ACAATCTTGA CGCTGTTCCT TTGGCAGCTT TGTCTTCTAC TACAGAAACT 
GCCTCTTTAC AGAGGCCTGC TGTCGTAGAA CGACCTGCTG TGAATGATTA TCTTAACCCA 
TGTGAACCAA ACCCTTGTGG AGCTGGTGTG TGTTCAGTGA AGGATGGAGT GGGACTTTGT 
CACTGCCCAC CTGGACTGCA TGGAGAAGAG TGCCAGTTTG AGGTTGATGT ATGCCATTCA 
AACCCTTGCG CCAATGGAGC CACCTGTGTG GAGGTAGAGG ACACTTTCAA ATGCTTATGT 
CTGCCCAGCT ACGAAGGAGA CCGGTGTGAG ACTGATTCAC ACAGCTGTGA GAAGGGCTGG 
ACTAAGTTTC AGGGCAACTG TTATTTACAT TTCTCGAAGA GAGAGACCTG GCTGGATGCT 
GAGCAACGCT GTCGAGACTT AAATGCTCAC CTGGTGAGCA TTAACACTCC AGAGGAGCAA 
GCCTTCGTCA ACTCAAATGC ACAAGACTAT CAGTGGATTG GACTAAATGA TAAAACAGTG 
GAAAATGACT TCCGCTGGTC AGATGGAACC CAGCTGCAAT TTGAGAACTG GAGACCAAAC 
CAGCCTGACA ATTATTTCAA CTCTGAAGAG GATTGCGTTG TGATGATCTG GCATGAAAAT 
GGCCAGTGGA ATGACGTGCC CTGCAACTAC CATCTGCCTT TTACATGCAA ATCAGGTCCA 
GTGACATGTG ATAAACCTCC GAAAGTGGAG AATGCCAAAA TGTTTGGAAA CAAGAAAGAG 
CGTTACCAGG TTAATTCTAT TATCAGATAC CAGTGCAGTG AAAACTTTAC ACAGCGCCAC 
CCACCTGTGA TCCGCTGCAT GGCAGACGGA CAGTGGGAGA AGCCAAAAGT TCAATGCATA 
CCCAAGGTCA AATTTGGACT TCACGACAAA TCTGTTCATC ACTCTCATAA AGCTAGAACA 
GCATTGGAGA AACAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333626.1
CDS	167..4807
Translation	MLSLLLLSMCLWVFSATASFDYIDTEPESPLRVSIPNELPLRPLMGDTLVLPCYFQDNTVNDPGAPTIAPLSHRIKWSLVTKEKTTNILVASEGIVSIEKRYMDRITLVGYPMTPTDASIKITELHSNDSGVYRCEVMQGIEDSHDIVDVQVQGIVFHYRAITTRYTLNFEKAKAACIQNSAVIATPEQLQAAYDEGFHQCDAGWLADQTVRYPIHDPREACYGDKYEFPGVRTYGVRDVNETYDVYCFAERMTGKVFHTTSPNKFTFEEAEAQCSKLGAKLATTGQLYLAWKGGMDVCNAGWLADRSVRYPINIARPQCGGGLLGVRTVYLFPNQTGYPHPDSRYDAFCYSETGDEGSGFFPLDLELTTDGDNGVLTVDTITESHTVISKPASTESEVQGEVVTYKPSVTTSVENPYTALDISHVLPSPPTSSVEKDPDFGSGLSQSNKTEDTMSATGVVFHYRGGNCSRYPFGFVEAQLACKSIGAVIASAQQLQAAYEAGYHQCDAGWLSDQTVRYPIVSPRHQCSGDMEQVPGVRSYGLRPADERYDVYCYVDKLRGQVFHASSFEGFTYGEAVAHCQGQNASLASTAHLYAAWKQGFDKCRAGWLLDRSVRYSINIPRQQCGGGRTGVHTVYLFPNQTGSPDLHSKFDAYCFKEDLDLPLNESESQETVTEEKIVSISSHPALHRPGTPIIAPSPTEPSGTEESVASSSGDLLSSGSGDQPSGSSVSSGDSSGTSSTDDLLTSGSGDQSGSGSSVTSGDYSGASGTDSLFSSGSGDRSSGSFSSSGDFSGASGTDDLLGSGSGEQPSGSFVSSGDFSGASGSGDMSSGSGSSSGSGSGLDILVTFSGIDTVFSGVGSTSGQLEEAGEGSTEILTFQLDQISGSGSGLGSGSGYSSEESGSTSDLSSSSGESGESGDLSGISSGSGFSEESSGFSGFASGIFPSGGSSDISFIDGSGIIMVDRPWMKISSFLTEQELGSGQVDFSGSGLFSGSGVSGDISGLSGEISGSSGDSSGISSDISGMSGDISGMSGDISGISGDITGMSGDLSGMSSSDHSGSGSGLAFSGVTFLGSGFTDLTEQSREQEASGLVFFGSGEGSGFRSGGYAIESGERSGDSASSDVIFFSNNDMVEMSNKPLQGMELGRGEVEYSGTVRLSSGSGDDQSVFISGHEWIPVTANTPSVKSTDPPPAELFDQGPSDLYSDTAGHLGPYLKPEEIINNLDAVPLAALSSTTETASLQRPAVVERPAVNDYLNPCEPNPCGAGVCSVKDGVGLCHCPPGLHGEECQFEVDVCHSNPCANGATCVEVEDTFKCLCLPSYEGDRCETDSHSCEKGWTKFQGNCYLHFSKRETWLDAEQRCRDLNAHLVSINTPEEQAFVNSNAQDYQWIGLNDKTVENDFRWSDGTQLQFENWRPNQPDNYFNSEEDCVVMIWHENGQWNDVPCNYHLPFTCKSGPVTCDKPPKVENAKMFGNKKERYQVNSIIRYQCSENFTQRHPPVIRCMADGQWEKPKVQCIPKVKFGLHDKSVHHSHKARTALEKQL

Target ORF information:

RefSeq Version	XM_021477951.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477951.1

ORF Insert Sequence:

ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC 
CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC 
AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC 
ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG 
AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT 
AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG 
ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT 
GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC 
AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG 
TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG 
GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG 
AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA 
ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC 
AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC 
GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT 
GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC 
CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC 
TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC 
ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG 
GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA 
CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC 
TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG 
GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG 
CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT 
GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC 
ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT 
TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG 
GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC 
TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA 
GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT 
ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC 
AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT 
CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG 
AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGGTACCC CTATCATTGC TCCCAGTCCT 
ACAGAACCTT CTGGAACTGA GGAATCTGTG GCCAGCAGTT CTGGCGATCT GTTGAGCAGT 
GGCAGTGGGG ATCAGCCAAG TGGGTCATCC GTTTCAAGTG GTGACTCCTC CGGGACAAGT 
AGCACTGATG ATCTTTTGAC AAGTGGGAGC GGGGATCAGA GTGGAAGTGG CTCATCTGTT 
ACAAGTGGAG ACTATTCTGG AGCCAGTGGC ACAGACAGTC TGTTCAGTAG TGGCAGTGGC 
GATCGGTCGA GTGGGTCATT CAGTTCCAGT GGAGACTTCT CTGGAGCCAG TGGGACTGAT 
GATTTGTTGG GCAGTGGGAG TGGGGAACAG CCAAGTGGGT CATTTGTGTC AAGCGGAGAC 
TTCTCTGGGG CCAGTGGAAG TGGGGATATG TCAAGTGGTT CTGGAAGCTC TTCAGGATCT 
GGTAGTGGTT TAGATATCTT AGTGACTTTT TCAGGGATTG ACACAGTTTT TTCAGGAGTT 
GGATCAACTT CAGGGCAACT TGAGGAGGCT GGGGAGGGAA GCACTGAGAT TCTTACATTC 
CAATTAGATC AAATCTCTGG ATCTGGATCG GGATTAGGCA GTGGGTCAGG GTACAGCTCT 
GAAGAAAGTG GATCTACCTC AGACCTTTCC AGCAGCTCAG GAGAGAGTGG TGAGAGTGGA 
GATCTTTCTG GCATCTCCTC AGGATCAGGG TTCAGTGAGG AGTCCTCAGG ATTCAGTGGT 
TTTGCATCAG GTATTTTTCC CTCTGGTGGA TCCAGTGACA TTTCATTCAT TGATGGCAGT 
GGAATCATCA TGGTGGACAG GCCGTGGATG AAGATATCTT CATTTTTAAC AGAACAAGAG 
CTGGGTAGTG GTCAAGTCGA TTTCAGTGGA TCAGGACTTT TCTCAGGGTC TGGGGTGAGT 
GGTGATATCT CTGGCTTAAG TGGTGAAATC TCTGGATCGA GTGGTGATAG TTCAGGTATA 
AGTAGTGACA TTTCAGGAAT GAGTGGTGAT ATCTCAGGAA TGAGTGGTGA TATCTCAGGA 
ATAAGTGGTG ATATTACAGG AATGAGTGGT GATCTCTCTG GTATGAGTAG CAGTGACCAC 
AGTGGCTCAG GGAGTGGTTT AGCATTTTCT GGGGTGACAT TTTTGGGGTC AGGCTTCACT 
GATCTCACAG AACAATCTCG TGAACAGGAA GCTTCTGGAC TTGTGTTCTT TGGGTCTGGT 
GAGGGAAGTG GATTTAGATC TGGAGGCTAT GCAATTGAAT CAGGGGAACG ATCAGGGGAC 
TCGGCTAGTA GTGATGTCAT CTTCTTCAGC AATAATGACA TGGTGGAAAT GTCAAACAAG 
CCACTTCAAG GAATGGAATT AGGAAGAGGG GAAGTGGAAT ATAGTGGCAC AGTCAGACTG 
TCCTCAGGGA GTGGAGATGA CCAAAGTGTC TTTATAAGCG GGCATGAATG GATCCCAGTC 
ACTGCTAATA CACCATCTGT AAAATCCACT GATCCTCCCC CAGCTGAATT ATTTGATCAA 
GGTCCATCAG ACCTGTACAG TGATACTGCA GGACACCTAG GTCCTTATTT AAAACCAGAA 
GAGATCATCA ACAATCTTGA CGCTGTTCCT TTGGCAGCTT TGTCTTCTAC TACAGAAACT 
GCCTCTTTAC AGAGGCCTGC TGTCGTAGAA CGACCTGCTG TGAATGATTA TCTTAACCCA 
TGTGAACCAA ACCCTTGTGG AGCTGGTGTG TGTTCAGTGA AGGATGGAGT GGGACTTTGT 
CACTGCCCAC CTGGACTGCA TGGAGAAGAG TGCCAGTTTG AGGTTGATGT ATGCCATTCA 
AACCCTTGCG CCAATGGAGC CACCTGTGTG GAGGTAGAGG ACACTTTCAA ATGCTTATGT 
CTGCCCAGCT ACGAAGGAGA CCGGTGTGAG ACTGATTCAC ACAGCTGTGA GAAGGGCTGG 
ACTAAGTTTC AGGGCAACTG TTATTTACAT TTCTCGAAGA GAGAGACCTG GCTGGATGCT 
GAGCAACGCT GTCGAGACTT AAATGCTCAC CTGGTGAGCA TTAACACTCC AGAGGAGCAA 
GCCTTCGTCA ACTCAAATGC ACAAGACTAT CAGTGGATTG GACTAAATGA TAAAACAGTG 
GAAAATGACT TCCGCTGGTC AGATGGAACC CAGCTGCAAT TTGAGAACTG GAGACCAAAC 
CAGCCTGACA ATTATTTCAA CTCTGAAGAG GATTGCGTTG TGATGATCTG GCATGAAAAT 
GGCCAGTGGA ATGACGTGCC CTGCAACTAC CATCTGCCTT TTACATGCAA ATCAGGTCCA 
GTGACATGTG ATAAACCTCC GAAAGTGGAG AATGCCAAAA TGTTTGGAAA CAAGAAAGAG 
CGTTACCAGG TTAATTCTAT TATCAGATAC CAGTGCAGTG AAAACTTTAC ACAGCGCCAC 
CCACCTGTGA TCCGCTGCAT GGCAGACGGA CAGTGGGAGA AGCCAAAAGT TCAATGCATA 
CCCAAGGTCA AATTTGGACT TCACGACAAA TCTGTTCATC ACTCTCATAA AGCTAGAACA 
GCATTGGAGA AACAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

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1-732-210-0262

acana cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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