col4a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col4a2
Entrez Gene ID	553355
Full Name	collagen, type IV, alpha 2
Synonyms	zCanstatin
General protein information	Preferred Names collagen, type IV, alpha 2 Names collagen alpha-2(IV) chain type IV collagen alpha 2 chain
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478837.1	XP_021334512.1	collagen alpha-2(IV) chain isoform X1
XM_017357758.2	XP_017213247.1	collagen alpha-2(IV) chain isoform X1
XM_021478840.1	XP_021334515.1	collagen alpha-2(IV) chain isoform X2
XM_017357758.1	XP_017213247.1	collagen alpha-2(IV) chain
XM_021478838.1	XP_021334513.1	collagen alpha-2(IV) chain isoform X1
XM_021478839.1	XP_021334514.1	collagen alpha-2(IV) chain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology

The following col4a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144045	XM_021478840.1 Latest version!	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa144045	XM_021478839.1 Latest version!	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa60301	XM_021478837.1 Latest version!	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60301	XM_017357758.2 Latest version!	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60301	XM_021478838.1 Latest version!	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
ODa60301	XM_017357758.1	Danio rerio collagen, type IV, alpha 2 (col4a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col4a2 ( XM_021478840.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334515.1 Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X5, mRNA.

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CloneID	ODa144045
Clone ID Related Accession (Same CDS sequence)	XM_021478840.1 , XM_021478839.1
Accession Version	XM_021478840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAGCGAC TTACTTAGAT GGCGTCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334515.1
CDS	265..5175
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFASDLLRWRL

Target ORF information:

RefSeq Version	XM_021478840.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478840.1

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAGCGAC TTACTTAGAT GGCGTCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_021478839.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334514.1 Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.

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CloneID	ODa144045
Clone ID Related Accession (Same CDS sequence)	XM_021478840.1 , XM_021478839.1
Accession Version	XM_021478839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAGCGAC TTACTTAGAT GGCGTCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334514.1
CDS	265..5175
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFASDLLRWRL

Target ORF information:

RefSeq Version	XM_021478839.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478839.1

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAGCGAC TTACTTAGAT GGCGTCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_021478837.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334512.1 Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa60301
Clone ID Related Accession (Same CDS sequence)	XM_021478837.1 , XM_017357758.2 , XM_017357758.1 , XM_021478838.1
Accession Version	XM_021478837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334512.1
CDS	265..5277
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFANKHSFWLTSIDESFQSSPSSETLKAGQLLSRISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_021478837.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478837.1

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_017357758.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213247.1 Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

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CloneID	ODa60301
Clone ID Related Accession (Same CDS sequence)	XM_021478837.1 , XM_017357758.2 , XM_017357758.1 , XM_021478838.1
Accession Version	XM_017357758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213247.1
CDS	265..5277
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFANKHSFWLTSIDESFQSSPSSETLKAGQLLSRISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_017357758.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357758.2

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_017357758.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213247.1 Danio rerio collagen, type IV, alpha 2 (col4a2), mRNA.

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CloneID	ODa60301
Clone ID Related Accession (Same CDS sequence)	XM_021478837.1 , XM_017357758.2 , XM_017357758.1 , XM_021478838.1
Accession Version	XM_017357758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(IV) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213247.1
CDS	279..5291
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFANKHSFWLTSIDESFQSSPSSETLKAGQLLSRISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_017357758.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357758.1

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_021478838.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334513.1 Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.

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CloneID	ODa60301
Clone ID Related Accession (Same CDS sequence)	XM_021478837.1 , XM_017357758.2 , XM_017357758.1 , XM_021478838.1
Accession Version	XM_021478838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334513.1
CDS	163..5175
Translation	MEGENHLQHWNTLRCFLLCLVVLILTTDVNAGVKKSTGPCGGRDCSGGCQCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKGEHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPSGARGKAGPDGCNGTRGDSGMPGFPGLEGGQGTPGWPGMKGEKGDPLEVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGLKGAKGQKGERTKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLGYLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGPPGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQPGPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKPPPGPPGPPGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGDPGDPGHSGFPGEQGRPGIDGRDGEPGFRGPPGEGPVGEPGAKGYSGPPGRSGLIGPKGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGDPGPPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTKGVKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPGLPGHRGDTGLTGDPGLPGYFGMEGMPGMTGLKGESGPPGLQGEQGRPGPPGTFGFPGQTGYTGPPGPPGNIGEPGPSGRRGDQGEPGLIGARGVKGAIGDPGNIGVTGNIGPPGDQGETGGPGFFGLPGLKGSKGGQGMTGFPGKTGERGLKGFSGTKGEIGLPGHQGAKGAPGSPGQKGDRGLTGPPGDKPVIHITPHMKEVMKGSKGDHGNMGDPGFTGPRGTKGFPGIPGGEGKDGHPGEPSVVKGVKGLPGEPGLEGPKGMPGPTGLPGIEGFPGMSGPKGNKGSSGAYGRPGDSGIKGFKGDLGPTISLPGSTGLRGETGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGRSGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNPGTKGELGETGIPSTTTGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQGDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGDRGNVGDFGLFGEKGVQGDRGEKGSSGESGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYPPVPQKLPGEQGPPGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGMPGLPGIPGSPGFRGEPGSMGHSGLQGEQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQDLGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEAPSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNGAKGTCHYFANKHSFWLTSIDESFQSSPSSETLKAGQLLSRISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_021478838.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478838.1

ORF Insert Sequence:

ATGGAGGGTG AGAATCATCT GCAGCACTGG AACACATTAA GGTGTTTTCT GTTGTGTCTT 
GTAGTCCTTA TCCTTACGAC AGATGTCAAC GCAGGAGTAA AGAAGAGCAC TGGACCATGT 
GGAGGAAGAG ACTGCAGTGG TGGGTGTCAG TGTTACCCTG AGAAGGGCGC AAGGGGATTG 
CCTGGACCAC TTGGCCCCCA GGGTCCCACA GGCCCTAAAG GGCGACAGGG AGAGCCTGGC 
CTTCAGGGCC CTAAAGGAAT GAAGGGAGAG CATGGCGAAG CAGGCTTCGT GGGTCCAAAA 
GGAAGTGTGG GAATGGAAGG GGTTCCAGGG TTTAATGGAG CTGATGGTGT ACCTGGCCAT 
CCTGGACCAT CTGGAGCTCG AGGTAAAGCA GGACCTGATG GCTGTAATGG AACCCGGGGA 
GATTCAGGAA TGCCGGGCTT CCCAGGTTTA GAAGGCGGAC AAGGAACACC AGGCTGGCCA 
GGAATGAAGG GGGAGAAAGG TGATCCTTTG GAGGTTTGGG TCTATATGGA AAGATTCAGG 
GGAGATCCAG GGCCAGCTGG TTTCCCAGGG ATTGTTGGAC CGACTGGTGA CCCAGGATAC 
AGAGGATTGC CAGGATTCCA AGGTCCTTGG GGTCCTCAAG GTCTGAAAGG TGCCAAGGGA 
CAAAAGGGTG AAAGAACTAA AATCTTAAAA GGAGTCAAAG GAGAATTGGG AGAAATTGGA 
GAGCCAGGCC CACCAGGAGT TTTCTCGCAA ACCAGCCCAA TCCCTTCAGA CTGGCAGGGT 
GAAAAAGGAA AAATGGGTTC TAAAGGAGAT AAGGGAGATT TGGGATATTT ATCCACAAAA 
GCAGAGAGCG GCGTGTCAGG GTTTGCAGGA GCTCGGGGAA ACCCAGGATT AGATGGCTGG 
CCAGGACCTA GGGGTGACAC TGGACTGCCA GGTTTCCCAG GAAATAATGG CCGCAAGGGA 
GACGTAGGAG AACCCGGGGA GCTAGTAGGA AGTCCATTTG ATGATACTGG AGTGGGTCCT 
CCAGGACCTC CAGGAGAGCG TGGCCCTGTT GGAAATTATG GCCGTAAAGG TGCTAAAGGA 
CAACCAGGTC CTCCAGGACC CCCTCCCTAT GGACAACAAA CAGTCGAATT GTGGGGTCCA 
GAAGGACCTC GGGGGCCAAA GGGAGCCAGT GGTGAAACTG GAGAACCTGG TAATCCTGCC 
ACTCAGCCTG GGCCACCTGG TCCTGATGGG TCACCTGGAA GTGTTGGGCC ACCTGGACCC 
AAGGGATCAT TGGAAGAGTA CTTCAAAGGT TCTCCTGGAC ACCGTGGACG TCCAGGGAGT 
GCAGGAAAAA AGGGGCCAAA GGGTGACCAT GGTCTGTGCG AATGCAGTGT AAAACCCCCA 
CCTGGTCCCC CGGGACCTCC TGGTGATTCT GGGGATCCTG GGATGGCAGG TGAGTGGGGT 
CAGCAGGGTG ATCAGGGAGA TCCAGGAACC AAAGGTGCCA ATGGTTTGCC TGGTTTTCCT 
GGCACAGAAG GATTACCTGG GCCCAAAGGC AGAAAAGGAG AGCTTATGGA AGCTGTGCAG 
AAAGGATCAG CTGGTGACCC AGGAGACCCA GGTCACAGTG GTTTCCCTGG AGAACAGGGA 
AGACCAGGGA TAGATGGCAG AGATGGTGAA CCAGGTTTTA GAGGGCCTCC TGGTGAAGGT 
CCTGTTGGTG AACCTGGAGC AAAGGGATAC TCAGGTCCAC CAGGTCGTTC AGGTCTAATC 
GGCCCAAAAG GAAATCCTGG CCAAGTTCTC GGAGCAACTA AAGGTTTAAG TGGCTTGCCT 
GGAGATGATG GACAGACTGG TCCTGATGGG AGAACAGGGC CTCCTGGTCC TCCAGGAGAC 
TGTAGCTCAA GAGGAGGAAG TCAGTGGAGT ATCCCAGTGG AGTGTATTGG AGATCCTGGG 
CCACCTGGAG AGAGTGGGCT ACCTGGACCA CGAGGGTTGG AAGGATTTCC AGGTGTACCA 
GGACCTAAAG GCACAGCAGG TTTTTCTGGA GATCTCGGGG ATAAAGGAGA GCGAGGTGAA 
CCAGGAAGAG GAGGCCCACC TGGACCAAAG GGTTTTACTG GACCTCGGGG AGATTTAGGA 
TATCCTGGCA CCAAAGGTGT GAAAGGTCCC CCAGGTTTAT CTGGTAAACC AGGTTTGGAT 
GGAGCTTCAG GGGGAAAGGG TGAACATGGT GAAGTATTTG GAGCATCTTC TGGTGCCCCT 
GGAGATCCGG GACTGCCAGG ACATAGAGGA GACACCGGCC TGACTGGAGA CCCAGGCTTA 
CCAGGATACT TTGGTATGGA GGGTATGCCA GGTATGACTG GCCTTAAAGG TGAAAGTGGC 
CCTCCAGGCC TTCAAGGAGA GCAAGGACGA CCTGGACCAC CAGGAACATT TGGTTTTCCT 
GGGCAGACTG GGTACACAGG ACCTCCTGGA CCACCTGGAA ATATTGGAGA ACCAGGTCCA 
AGTGGAAGAA GGGGAGATCA GGGAGAGCCA GGACTAATAG GAGCAAGGGG AGTAAAGGGT 
GCCATTGGAG ACCCTGGCAA TATTGGTGTG ACAGGAAATA TCGGGCCCCC GGGAGATCAA 
GGAGAAACGG GTGGACCTGG TTTTTTTGGT CTCCCAGGCT TAAAAGGTTC CAAAGGTGGT 
CAGGGCATGA CCGGTTTCCC TGGAAAGACC GGTGAGAGAG GTTTGAAAGG ATTTTCAGGA 
ACGAAAGGGG AGATCGGTTT ACCAGGACAC CAAGGTGCAA AAGGAGCTCC TGGTTCTCCA 
GGTCAAAAAG GTGACCGAGG GCTGACGGGG CCTCCAGGTG ACAAACCAGT CATACATATA 
ACCCCACATA TGAAAGAGGT CATGAAGGGA TCTAAAGGTG ACCATGGAAA TATGGGAGAT 
CCTGGCTTCA CTGGTCCCAG AGGTACAAAA GGCTTTCCAG GTATACCAGG TGGTGAGGGA 
AAAGATGGAC ATCCAGGTGA GCCCAGTGTT GTAAAAGGTG TAAAAGGTCT TCCGGGAGAA 
CCGGGTTTAG AAGGGCCCAA GGGAATGCCA GGGCCTACCG GACTCCCTGG AATTGAAGGT 
TTCCCTGGAA TGTCTGGACC CAAGGGCAAC AAAGGCTCCT CTGGTGCCTA TGGACGACCT 
GGAGATTCTG GAATCAAAGG ATTCAAAGGT GACTTGGGGC CTACCATCAG TCTTCCAGGA 
TCAACAGGAC TGAGAGGTGA AACTGGACAT CCTGGAAGTA CAGGTGCGAA GGGACTCTAT 
GGTATGCCTG GAGAAAGAGG AAGTTCAGGA TTAGATGGAA TTGAAGGGAT GAAAGGCTAC 
CAAGGTGAAC CTGGGACAGT TGGACCACCA GGCTCTGATG GACTACAGGG TTTTCCAGGA 
AGCCAAGGTC ACAAGGGCAG GTCAGGAGAC CCAGGACAGA CAGGAACCAC TGGAATCCAA 
GGCCAGCCAG GCCAAAAAGG ATTTCCTGGT CTTAAAGGCA TTTTTGGGTT GGATGGACTG 
AAAGGTCAAA AGGGTAACCA GGGTGTCCCA GGTACTGACA ACTGGGGCCA ACCAGGAAAC 
CCAGGAACTA AAGGAGAGTT AGGAGAGACT GGCATCCCCA GCACAACAAC TGGTGTCCCC 
GGGTCACTGG GACACAAGGG TACCACTGGA GATTCAGGAA ATAAGGGTGA AATTGGGCAG 
CGAGGTGTAC CGGGGCCTCA AGGTCAGCAA GGATTTTCTG ATATAAAAGG AACGCAGGGC 
GACTATGGTT TTCCAGGCAT AAAAGGAATA CCAGGTTTTC CTGGTACCAG AGGAACCCCA 
GGGATCCCAT CTCCCTTCGG AGCAAAAGGA GATAGAGGAA ATGTTGGTGA TTTCGGATTA 
TTTGGAGAAA AAGGAGTCCA GGGTGACAGA GGAGAAAAGG GAAGTTCTGG GGAATCTGGA 
GTGTCAGGAA GAAAAGGTGA TAAAGGAGAG CCAGGGATGA TGGGATTTCC AGGAAGACGT 
GGTTTTCAAG GTGACAGGGG CCCTTTTGGA CCTAAAGGAG ATACCGGACC CCCAGGTTTT 
CCTGGATTAC CTGGGGCTAA AGGATATCCA CCAGTTCCAC AGAAACTTCC AGGGGAGCAA 
GGGCCGCCTG GTCAGATGGG TATTCAGGGA CCATCGGGGC GCAATGGAAA CCCAGGCCCT 
GCGGGACCTC CTGGTGATTC AGGATTTGTA GGTCCAAGTG GACACAAGGG GATGCCAGGT 
CTTCCTGGAA TACCAGGAAG TCCAGGCTTC CGTGGAGAAC CTGGATCAAT GGGCCACTCT 
GGTCTGCAAG GCGAACAAGG GACTCGAGGA CGACCTGGTA TGCCCGGTCC TCTTGGAATG 
GCTGGCCGCA GTGTGAACGT TGGCTATCTG CTGGTGAAGC ACAGTCAGTC TGAGGAGATC 
CCCATGTGTC CTCAGGGCAT GTCCTTGTTA TGGATGGGTT ACAGTCTTCT GTACTTCGAG 
GGCCAGGAAA AGGCCCACAA CCAAGACCTG GGTCTAGCCG GATCATGCCT CCCCCGTTTT 
AACACCATGC CCTTTCTGTA CTGTAATCCG GGAGACATAT GCTACTACGC CAGCCGCAAT 
GACAAATCCT ATTGGCTGTC CACAACCCAG CCGATTCCCA TGATGCCCGT AGAGGAGTCT 
GAAATAAAGC CGTACATCAG CCGCTGCTCT GTGTGTGAAG CTCCATCTGT GGCCATCGCC 
GTACACAGTC AAGATACAAC CATTCCAAAC TGCCCAGTCG GCTGGAGAAG CCTCTGGATT 
GGCTATTCCT TCCTCATGCA CACAGCAGCT GGAGATGAAG GTGGAGGTCA GTCTCTGGCG 
TCTCCAGGCT CGTGTCTTGA GGATTTCCGC ACCACCCCCT TCATCGAGTG CAACGGGGCC 
AAGGGAACGT GTCACTACTT CGCCAACAAG CACAGCTTCT GGCTGACGTC TATCGACGAG 
TCTTTCCAGA GCTCCCCTTC CTCTGAGACA CTCAAAGCCG GTCAGCTCCT GTCCCGCATC 
AGCCGCTGCC AAGTGTGCAT GAAGAACCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col4a2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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