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nle1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol nle1
Entrez Gene ID 553606
Full Name notchless homolog 1 (Drosophila)
Synonyms fc12b09,fc45f01,wu:fc12b09,wu:fc45f01,zgc:110281
General protein information
Preferred Names

notchless homolog 1 (Drosophila)

Names

notchless protein homolog 1
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001328517.1 NP_001315446.1 notchless protein homolog 1
NM_001328516.1 NP_001315445.1 notchless protein homolog 1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) NLE1 XP_001174396.2
NLE1 XP_001113853.1
Mus musculus (house mouse) Nle1 NP_663406.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002221 XP_307962.4
Schizosaccharomyces pombe (fission yeast) SPCC18.05c NP_588384.1
Neurospora crassa NCU00794 XP_964950.1
Rattus norvegicus (Norway rat) Nle1 NP_001121006.1
Caenorhabditis elegans (roundworm) W07E6.2 NP_493745.1
Kluyveromyces lactis KLLA0C08547g XP_452581.1
Eremothecium gossypii AGOS_AER263C NP_985120.1
Homo sapiens (human) NLE1 NP_060566.2
Canis lupus familiaris (dog) NLE1 XP_005624858.1
Danio rerio (zebrafish) nle1 NP_001018418.1
Drosophila melanogaster (fruit fly) Nle NP_477294.2
Gallus gallus (chicken) NLE1 XP_415857.3
Saccharomyces cerevisiae (baker's yeast) RSA4 NP_009997.2
Oryza sativa (rice) Os11g0594200 NP_001068204.1
Bos taurus (cattle) NLE1 NP_001014887.1
Magnaporthe oryzae (rice blast fungus) MGG_08708 XP_003719269.1
Arabidopsis thaliana (thale cress) AT5G52820 NP_200094.1
Oryza sativa (rice) Os10g0104500 NP_001064020.1
Xenopus tropicalis (tropical clawed frog) nle1 NP_001072540.1

Gene Ontology


Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa16090 NM_001328517.1
Latest version!		 Danio rerio notchless homolog 1 (Drosophila) (nle1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
ODa16090 NM_001328516.1
Latest version!		 Danio rerio notchless homolog 1 (Drosophila) (nle1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00

ORF Online Only Promotion

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2-4 Clone 40% OFF
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa16090
Clone ID Related Accession (Same CDS sequence) NM_001328517.1 , NM_001328516.1
Accession Version NM_001328517.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-23
Organism Danio rerio(zebrafish)
Product notchless protein homolog 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR926463.9. On Jun 16, 2016 this sequence version replaced XM_005155261.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01009948.1, GDQQ01003958.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGCGCGG ATGTGGAGCG TGTGTTGATC CAGCTCCAGG ATGAGGCCGG TGAGGTGCTC 
GGGTCTCCCT TTGATGTGCC GCTGGACATC AGTCCGGACA AACTGCAGCT GGTCTGCAAT 
GCGCTCTTAC AGAAGGAAGA GCCGGTGCCT TTCTTGTTCT TTGTGAAAGA TGCAGAGCTG 
GTGTCGTCTC TGGGCTCCTG CATTCAGACA TTGGGTCTGG AGACGGAGCA GGTGCTACCG 
GTGGTGTATC AGCCACAGGC TGTATTTCGT GTGCGGGCTG TGGCTCGGTG CACCAGCTCT 
CTGGAGGGCC ACACTGAGGC CGTCATATCT GTGGCATTCA GCCCGACTGG AAAATATCTG 
GCCAGTGGAT CTGGAGACAC GACAGTGAGG TTTTGGGATC TGAGCACAGA AACACCACAT 
CACACTTCTA GAGGACACAC ACACTGGGTT TTGTCCATTG CTTGGTCTCC AGATGGAAAG 
AAATTAGCAT CAGGCTGCAA AAACAGTCAG ATTTTCTTGT GGGATCCAGT CACAGGCAAA 
CAGATTGGAA AGACTTTGAC AGGACATACA AAGTGGATCA CCTGGCTGTG CTGGGAACCT 
CTCCATTTAA ACCCAGAGTG TCGCTATTTG GCAAGCACCA GTAAAGACTG TACGATTCGC 
ATATGGGACA CTGTTTTGGG TCACTATGAC AAGATCCTAA CAGGACACAC ACACTCTGTG 
ACGTGTGTGA AATGGGGAGG CGATGGTCTC CTCTACACAT CATCTCAGGA TAGGACTATT 
AAAGTCTGGA GAGCCAAAGA TGGTGTTCAG TGTCGTACAC TGCAAGGTCA CGCTCACTGG 
GTGAACACAC TGGCCCTCAG CACTGACTAT GTCCTGCGTA CAGGAGCATT TGAACCCGCG 
AACGCAACCA TAAACCCTCA GGATCTCACC GGATCATTGG AAGAGATCAA GGAAAAGGCT 
TTGAAGCGCT ACAACAGTGT CAGAGGTGAA GGTCATGAGA GGCTGGTTTC TGGATCAGAC 
GACTTCACCA TGTTCTTATG GAACCCAGCA GAGGACAAGA AGCCGGTCGC TCGTCTAACA 
GGTCACCAAG CATTGGTCAA TGAGGTGCTC TTCTCACCCG ACACACGACT CATAGCCAGC 
GCTTCCTTCG ACAAGTCCAT TAAGATCTGG GATGGAAAAA CAGGAAAATA TCTGAATTCA 
CTGCGGGGCC ACGTCGGGCC TGTGTACCAG GTGGCATGGT CAGCAGACAG TCGCCTGTTG 
GTCAGCGGCA GCAGTGACAG CACTCTGAAG GTGTGGGACA TCAAAACGGG AAAACTGAAC 
GCAGACCTGC CCGGCCATGC TGACGAGGTT TTTGCAGTCG ACTGGAGTCC TGATGGACAG 
CGAGTGGCCA GCGGAGGGAA AGACAAATGT TTAAGGATAT GGAGGAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001315446.1
CDS92..1522
Translation

Target ORF information:

RefSeq Version NM_001328517.1
Organism Danio rerio(zebrafish)
Definition Danio rerio notchless homolog 1 (Drosophila) (nle1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001328517.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGCGCGG ATGTGGAGCG TGTGTTGATC CAGCTCCAGG ATGAGGCCGG TGAGGTGCTC 
GGGTCTCCCT TTGATGTGCC GCTGGACATC AGTCCGGACA AACTGCAGCT GGTCTGCAAT 
GCGCTCTTAC AGAAGGAAGA GCCGGTGCCT TTCTTGTTCT TTGTGAAAGA TGCAGAGCTG 
GTGTCGTCTC TGGGCTCCTG CATTCAGACA TTGGGTCTGG AGACGGAGCA GGTGCTACCG 
GTGGTGTATC AGCCACAGGC TGTATTTCGT GTGCGGGCTG TGGCTCGGTG CACCAGCTCT 
CTGGAGGGCC ACACTGAGGC CGTCATATCT GTGGCATTCA GCCCGACTGG AAAATATCTG 
GCCAGTGGAT CTGGAGACAC GACAGTGAGG TTTTGGGATC TGAGCACAGA AACACCACAT 
CACACTTCTA GAGGACACAC ACACTGGGTT TTGTCCATTG CTTGGTCTCC AGATGGAAAG 
AAATTAGCAT CAGGCTGCAA AAACAGTCAG ATTTTCTTGT GGGATCCAGT CACAGGCAAA 
CAGATTGGAA AGACTTTGAC AGGACATACA AAGTGGATCA CCTGGCTGTG CTGGGAACCT 
CTCCATTTAA ACCCAGAGTG TCGCTATTTG GCAAGCACCA GTAAAGACTG TACGATTCGC 
ATATGGGACA CTGTTTTGGG TCACTATGAC AAGATCCTAA CAGGACACAC ACACTCTGTG 
ACGTGTGTGA AATGGGGAGG CGATGGTCTC CTCTACACAT CATCTCAGGA TAGGACTATT 
AAAGTCTGGA GAGCCAAAGA TGGTGTTCAG TGTCGTACAC TGCAAGGTCA CGCTCACTGG 
GTGAACACAC TGGCCCTCAG CACTGACTAT GTCCTGCGTA CAGGAGCATT TGAACCCGCG 
AACGCAACCA TAAACCCTCA GGATCTCACC GGATCATTGG AAGAGATCAA GGAAAAGGCT 
TTGAAGCGCT ACAACAGTGT CAGAGGTGAA GGTCATGAGA GGCTGGTTTC TGGATCAGAC 
GACTTCACCA TGTTCTTATG GAACCCAGCA GAGGACAAGA AGCCGGTCGC TCGTCTAACA 
GGTCACCAAG CATTGGTCAA TGAGGTGCTC TTCTCACCCG ACACACGACT CATAGCCAGC 
GCTTCCTTCG ACAAGTCCAT TAAGATCTGG GATGGAAAAA CAGGAAAATA TCTGAATTCA 
CTGCGGGGCC ACGTCGGGCC TGTGTACCAG GTGGCATGGT CAGCAGACAG TCGCCTGTTG 
GTCAGCGGCA GCAGTGACAG CACTCTGAAG GTGTGGGACA TCAAAACGGG AAAACTGAAC 
GCAGACCTGC CCGGCCATGC TGACGAGGTT TTTGCAGTCG ACTGGAGTCC TGATGGACAG 
CGAGTGGCCA GCGGAGGGAA AGACAAATGT TTAAGGATAT GGAGGAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa16090
Clone ID Related Accession (Same CDS sequence) NM_001328517.1 , NM_001328516.1
Accession Version NM_001328516.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-23
Organism Danio rerio(zebrafish)
Product notchless protein homolog 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR926463.9. On Jun 16, 2016 this sequence version replaced XM_005155262.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC154356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGCGCGG ATGTGGAGCG TGTGTTGATC CAGCTCCAGG ATGAGGCCGG TGAGGTGCTC 
GGGTCTCCCT TTGATGTGCC GCTGGACATC AGTCCGGACA AACTGCAGCT GGTCTGCAAT 
GCGCTCTTAC AGAAGGAAGA GCCGGTGCCT TTCTTGTTCT TTGTGAAAGA TGCAGAGCTG 
GTGTCGTCTC TGGGCTCCTG CATTCAGACA TTGGGTCTGG AGACGGAGCA GGTGCTACCG 
GTGGTGTATC AGCCACAGGC TGTATTTCGT GTGCGGGCTG TGGCTCGGTG CACCAGCTCT 
CTGGAGGGCC ACACTGAGGC CGTCATATCT GTGGCATTCA GCCCGACTGG AAAATATCTG 
GCCAGTGGAT CTGGAGACAC GACAGTGAGG TTTTGGGATC TGAGCACAGA AACACCACAT 
CACACTTCTA GAGGACACAC ACACTGGGTT TTGTCCATTG CTTGGTCTCC AGATGGAAAG 
AAATTAGCAT CAGGCTGCAA AAACAGTCAG ATTTTCTTGT GGGATCCAGT CACAGGCAAA 
CAGATTGGAA AGACTTTGAC AGGACATACA AAGTGGATCA CCTGGCTGTG CTGGGAACCT 
CTCCATTTAA ACCCAGAGTG TCGCTATTTG GCAAGCACCA GTAAAGACTG TACGATTCGC 
ATATGGGACA CTGTTTTGGG TCACTATGAC AAGATCCTAA CAGGACACAC ACACTCTGTG 
ACGTGTGTGA AATGGGGAGG CGATGGTCTC CTCTACACAT CATCTCAGGA TAGGACTATT 
AAAGTCTGGA GAGCCAAAGA TGGTGTTCAG TGTCGTACAC TGCAAGGTCA CGCTCACTGG 
GTGAACACAC TGGCCCTCAG CACTGACTAT GTCCTGCGTA CAGGAGCATT TGAACCCGCG 
AACGCAACCA TAAACCCTCA GGATCTCACC GGATCATTGG AAGAGATCAA GGAAAAGGCT 
TTGAAGCGCT ACAACAGTGT CAGAGGTGAA GGTCATGAGA GGCTGGTTTC TGGATCAGAC 
GACTTCACCA TGTTCTTATG GAACCCAGCA GAGGACAAGA AGCCGGTCGC TCGTCTAACA 
GGTCACCAAG CATTGGTCAA TGAGGTGCTC TTCTCACCCG ACACACGACT CATAGCCAGC 
GCTTCCTTCG ACAAGTCCAT TAAGATCTGG GATGGAAAAA CAGGAAAATA TCTGAATTCA 
CTGCGGGGCC ACGTCGGGCC TGTGTACCAG GTGGCATGGT CAGCAGACAG TCGCCTGTTG 
GTCAGCGGCA GCAGTGACAG CACTCTGAAG GTGTGGGACA TCAAAACGGG AAAACTGAAC 
GCAGACCTGC CCGGCCATGC TGACGAGGTT TTTGCAGTCG ACTGGAGTCC TGATGGACAG 
CGAGTGGCCA GCGGAGGGAA AGACAAATGT TTAAGGATAT GGAGGAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001315445.1
CDS135..1565
Translation

Target ORF information:

RefSeq Version NM_001328516.1
Organism Danio rerio(zebrafish)
Definition Danio rerio notchless homolog 1 (Drosophila) (nle1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001328516.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGCGCGG ATGTGGAGCG TGTGTTGATC CAGCTCCAGG ATGAGGCCGG TGAGGTGCTC 
GGGTCTCCCT TTGATGTGCC GCTGGACATC AGTCCGGACA AACTGCAGCT GGTCTGCAAT 
GCGCTCTTAC AGAAGGAAGA GCCGGTGCCT TTCTTGTTCT TTGTGAAAGA TGCAGAGCTG 
GTGTCGTCTC TGGGCTCCTG CATTCAGACA TTGGGTCTGG AGACGGAGCA GGTGCTACCG 
GTGGTGTATC AGCCACAGGC TGTATTTCGT GTGCGGGCTG TGGCTCGGTG CACCAGCTCT 
CTGGAGGGCC ACACTGAGGC CGTCATATCT GTGGCATTCA GCCCGACTGG AAAATATCTG 
GCCAGTGGAT CTGGAGACAC GACAGTGAGG TTTTGGGATC TGAGCACAGA AACACCACAT 
CACACTTCTA GAGGACACAC ACACTGGGTT TTGTCCATTG CTTGGTCTCC AGATGGAAAG 
AAATTAGCAT CAGGCTGCAA AAACAGTCAG ATTTTCTTGT GGGATCCAGT CACAGGCAAA 
CAGATTGGAA AGACTTTGAC AGGACATACA AAGTGGATCA CCTGGCTGTG CTGGGAACCT 
CTCCATTTAA ACCCAGAGTG TCGCTATTTG GCAAGCACCA GTAAAGACTG TACGATTCGC 
ATATGGGACA CTGTTTTGGG TCACTATGAC AAGATCCTAA CAGGACACAC ACACTCTGTG 
ACGTGTGTGA AATGGGGAGG CGATGGTCTC CTCTACACAT CATCTCAGGA TAGGACTATT 
AAAGTCTGGA GAGCCAAAGA TGGTGTTCAG TGTCGTACAC TGCAAGGTCA CGCTCACTGG 
GTGAACACAC TGGCCCTCAG CACTGACTAT GTCCTGCGTA CAGGAGCATT TGAACCCGCG 
AACGCAACCA TAAACCCTCA GGATCTCACC GGATCATTGG AAGAGATCAA GGAAAAGGCT 
TTGAAGCGCT ACAACAGTGT CAGAGGTGAA GGTCATGAGA GGCTGGTTTC TGGATCAGAC 
GACTTCACCA TGTTCTTATG GAACCCAGCA GAGGACAAGA AGCCGGTCGC TCGTCTAACA 
GGTCACCAAG CATTGGTCAA TGAGGTGCTC TTCTCACCCG ACACACGACT CATAGCCAGC 
GCTTCCTTCG ACAAGTCCAT TAAGATCTGG GATGGAAAAA CAGGAAAATA TCTGAATTCA 
CTGCGGGGCC ACGTCGGGCC TGTGTACCAG GTGGCATGGT CAGCAGACAG TCGCCTGTTG 
GTCAGCGGCA GCAGTGACAG CACTCTGAAG GTGTGGGACA TCAAAACGGG AAAACTGAAC 
GCAGACCTGC CCGGCCATGC TGACGAGGTT TTTGCAGTCG ACTGGAGTCC TGATGGACAG 
CGAGTGGCCA GCGGAGGGAA AGACAAATGT TTAAGGATAT GGAGGAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


book

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS