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ngly1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol ngly1
Entrez Gene ID 553627
Full Name N-glycanase 1
Synonyms im:6895151,zgc:110561
General protein information
Preferred Names

N-glycanase 1

Names

peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
PNGase
peptide:N-glycanase
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001020601.1 NP_001018437.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Pathways from BioSystems


Homologies


Bos taurus (cattle) NGLY1 NP_001179716.1
Rattus norvegicus (Norway rat) Ngly1 NP_001014158.1
Neurospora crassa NCU00651 XP_965791.1
Mus musculus (house mouse) Ngly1 NP_067479.2
Danio rerio (zebrafish) ngly1 NP_001018437.1
Homo sapiens (human) NGLY1 NP_060767.2
NGLY1 XP_001092796.1
Canis lupus familiaris (dog) NGLY1 XP_003639867.1
Gallus gallus (chicken) NGLY1 NP_001026159.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007390 XP_308442.4
Caenorhabditis elegans (roundworm) png-1 NP_492913.1
Saccharomyces cerevisiae (baker's yeast) PNG1 NP_015229.1
Kluyveromyces lactis KLLA0E24223g XP_455043.1
Magnaporthe oryzae (rice blast fungus) MGG_03598 XP_003716307.1
Pan troglodytes (chimpanzee) NGLY1 XP_003309721.1
Schizosaccharomyces pombe (fission yeast) SPBC1709.14 NP_595447.1
Drosophila melanogaster (fruit fly) Pngl NP_724444.3
Eremothecium gossypii AGOS_ABR193W NP_983142.1
Xenopus tropicalis (tropical clawed frog) ngly1 XP_002932449.1

Gene Ontology


Bibliography

Related articles in PubMed

Molecular insights of organochlorine biocide-induced toxicity in zebrafish: Whole-adult-organism toxicogenomics, targeted gene expression and histological analyses.
Lui EY, Li C, Li ZH, Gong Z, Lam SH
Journal of genetics and genomics = Yi chuan xue bao43(8)525-8(2016 Aug)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ngly1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ngly1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa37856 NM_001020601.1
Latest version!		 Danio rerio N-glycanase 1 (ngly1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa37856
Clone ID Related Accession (Same CDS sequence) NM_001020601.1
Accession Version NM_001020601.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-23
Organism Danio rerio(zebrafish)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095313.1. ##Evidence-Data-START## Transcript exon combination :: BC095313.1, BC165632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476763, SAMEA4476792 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCTGGCT CTCAAGGAGT CCTTGCTTTG TGCGAAAATA CCACGGAAGT GTTTTTGGAC 
GTCTCTAAAT TGCTAATTAC ATACGCGGAC AACATCCTAA GGAACCCAAA TGAGGAGAAG 
TACAGATCAA TTCGTATTGG AAACCCAACA TTCTCCACCA AGCTTCTGCC TGTAAAAGGA 
GCAGTTGAAT GTCTCTTTGA GATGGGATTT GAGGAGGCTG AAACCCACCT GGTGTTTCCG 
AGGTCAGCAT CTGTGGAGAA GCTGAGGCAG GTGCGGGAGA CGATCGCTGC TGAGAGAGAC 
CAGAGACTTG GTGCGAGCAA TACTGCGTCC AGTCCACAGA ATGCCAGCGT TCCTCCAGTT 
CCTGCACCTC CACCATCACT ATCCTCCACT GCTTCTGCAG GCCCTGCTGC TGCTGCCGTC 
CCTACACCTG CACCTGCTTC TATACCATTC ACTTCAAGCA GTGCTACATT CCAAAGAACA 
GTACAGTCAA ACTTTCAACA TGTGTTAGTG TATGAGGATC CTGAATTACA GCAGAAAGCT 
CTGGAATGCA TACCTCATGA ACTGCTCAGG TCCAGGGCTA AAGAACGACT GAAACAAGCC 
AATGATGCAG ATGCAGCATG CAGTCTAGGT GAAGAAGACA TGTTAGTTCT TGACCTGCTG 
CAGTGGTTCA AAAGTGACTT CTTTTCCTGG GTGGATAATC TGCCATGTAT ACAGTGTGGA 
GGTAAAACCC AGCCTTCCGG ATCCCTCTCC CCGTCCAGTG ATGACCTGCA CTGGGATGCA 
GGCCGTGTAG AGAACCACTT TTGTCATACT TGCCAACTCT CCACTAGATT CCCAAGGTAC 
AATAACCCAG AGAAGCTTCT AGAGACCAGG AAAGGACGTT GTGGGGAATG GGCCAACTGT 
TTCACCCTGC TCTGCAGAGC CTTAGGCCTT GAAGCCAGAT ATATCTGGGA CAGCACAGAT 
CATGTGTGGA CAGAAGTGTA CTCTCAGTCT CAGCGCCGCT GGATACACTG TGACCCTTGT 
GAGAATGCAT GTGATAAACC CCTCCTGTAT GAAGTTGGCT GGGGCAAGAA ACTCTCCTAC 
ATCCTGGCCT TCTCCAAGGA TCAGGTGGCA GATGTGACGT GGAGATACTC CTGTAAGCAC 
CCAGAGGTTC TGTCCAGACG CACACAAGTC CAGGAGACGT GGCTGCTTCA TATGCTTAAC 
AAACTCAACG CTGAGAGGCA GCAGTTTCTA GGAGCAGAGA GAAAGCAGCA GCTGTTGCAA 
AGGCTGCTGG TGGAGCTGGT GGAGTTTATT TCTCCTAAAA GTCCTCAACC TGGAGAACTG 
GGTGGCCGCA TGTCAGGATC CTTGGCTTGG AGAGCAGCTC GAGGAGAAAC TGGTGCAAGC 
AATGCTAAAG AGGACACACA GGAAAATGTC TTCACACCCT CAGAATCTGA GAAGGAAAGC 
AAACGCTTCC ACATTTGTTA CAATGTGACC AAAGACTGTT ACTACAGAGT GAGCAATGGC 
CAAGAGACTA TTCAAGGATG GCAGAAAGGG GCCTGGAGGA CAGAAAACAT GTTCAGGAAA 
GAAGAGCATG ACTGGCAGAT GGTGTATCTG GCACGAACAG AGGGCGCTTC CTTTGGGAGG 
ATGAGCTGGA AGTTCAACTG CGCTCCAGCA GGAATGAAGA TGAAATCAGC ATCAGTCCGA 
GTCTTCAGCC AGACCTTCCA TTCAGGCACT GTGCGTTGGA GTTTAAGTAC CAACGAGACA 
ACCACAGAAT TCCCTGGAGA TGGTGAACTG CACTCATTTT CAAATGTGTC CGGAGGCACT 
GAGCTGATTG TGGAGGCCGA GCTCACTGGA GGAGATGGCA GCACTTCATG GCAACACGCT 
CAGCTCTTCA GACAGAGTTT AAAAGACACA GAGAAAGTTC TATTTGAAGT GATGGTTGAG 
ATGGAAAAGT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018437.1
CDS47..1981
Translation

Target ORF information:

RefSeq Version NM_001020601.1
Organism Danio rerio(zebrafish)
Definition Danio rerio N-glycanase 1 (ngly1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001020601.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCTGGCT CTCAAGGAGT CCTTGCTTTG TGCGAAAATA CCACGGAAGT GTTTTTGGAC 
GTCTCTAAAT TGCTAATTAC ATACGCGGAC AACATCCTAA GGAACCCAAA TGAGGAGAAG 
TACAGATCAA TTCGTATTGG AAACCCAACA TTCTCCACCA AGCTTCTGCC TGTAAAAGGA 
GCAGTTGAAT GTCTCTTTGA GATGGGATTT GAGGAGGCTG AAACCCACCT GGTGTTTCCG 
AGGTCAGCAT CTGTGGAGAA GCTGAGGCAG GTGCGGGAGA CGATCGCTGC TGAGAGAGAC 
CAGAGACTTG GTGCGAGCAA TACTGCGTCC AGTCCACAGA ATGCCAGCGT TCCTCCAGTT 
CCTGCACCTC CACCATCACT ATCCTCCACT GCTTCTGCAG GCCCTGCTGC TGCTGCCGTC 
CCTACACCTG CACCTGCTTC TATACCATTC ACTTCAAGCA GTGCTACATT CCAAAGAACA 
GTACAGTCAA ACTTTCAACA TGTGTTAGTG TATGAGGATC CTGAATTACA GCAGAAAGCT 
CTGGAATGCA TACCTCATGA ACTGCTCAGG TCCAGGGCTA AAGAACGACT GAAACAAGCC 
AATGATGCAG ATGCAGCATG CAGTCTAGGT GAAGAAGACA TGTTAGTTCT TGACCTGCTG 
CAGTGGTTCA AAAGTGACTT CTTTTCCTGG GTGGATAATC TGCCATGTAT ACAGTGTGGA 
GGTAAAACCC AGCCTTCCGG ATCCCTCTCC CCGTCCAGTG ATGACCTGCA CTGGGATGCA 
GGCCGTGTAG AGAACCACTT TTGTCATACT TGCCAACTCT CCACTAGATT CCCAAGGTAC 
AATAACCCAG AGAAGCTTCT AGAGACCAGG AAAGGACGTT GTGGGGAATG GGCCAACTGT 
TTCACCCTGC TCTGCAGAGC CTTAGGCCTT GAAGCCAGAT ATATCTGGGA CAGCACAGAT 
CATGTGTGGA CAGAAGTGTA CTCTCAGTCT CAGCGCCGCT GGATACACTG TGACCCTTGT 
GAGAATGCAT GTGATAAACC CCTCCTGTAT GAAGTTGGCT GGGGCAAGAA ACTCTCCTAC 
ATCCTGGCCT TCTCCAAGGA TCAGGTGGCA GATGTGACGT GGAGATACTC CTGTAAGCAC 
CCAGAGGTTC TGTCCAGACG CACACAAGTC CAGGAGACGT GGCTGCTTCA TATGCTTAAC 
AAACTCAACG CTGAGAGGCA GCAGTTTCTA GGAGCAGAGA GAAAGCAGCA GCTGTTGCAA 
AGGCTGCTGG TGGAGCTGGT GGAGTTTATT TCTCCTAAAA GTCCTCAACC TGGAGAACTG 
GGTGGCCGCA TGTCAGGATC CTTGGCTTGG AGAGCAGCTC GAGGAGAAAC TGGTGCAAGC 
AATGCTAAAG AGGACACACA GGAAAATGTC TTCACACCCT CAGAATCTGA GAAGGAAAGC 
AAACGCTTCC ACATTTGTTA CAATGTGACC AAAGACTGTT ACTACAGAGT GAGCAATGGC 
CAAGAGACTA TTCAAGGATG GCAGAAAGGG GCCTGGAGGA CAGAAAACAT GTTCAGGAAA 
GAAGAGCATG ACTGGCAGAT GGTGTATCTG GCACGAACAG AGGGCGCTTC CTTTGGGAGG 
ATGAGCTGGA AGTTCAACTG CGCTCCAGCA GGAATGAAGA TGAAATCAGC ATCAGTCCGA 
GTCTTCAGCC AGACCTTCCA TTCAGGCACT GTGCGTTGGA GTTTAAGTAC CAACGAGACA 
ACCACAGAAT TCCCTGGAGA TGGTGAACTG CACTCATTTT CAAATGTGTC CGGAGGCACT 
GAGCTGATTG TGGAGGCCGA GCTCACTGGA GGAGATGGCA GCACTTCATG GCAACACGCT 
CAGCTCTTCA GACAGAGTTT AAAAGACACA GAGAAAGTTC TATTTGAAGT GATGGTTGAG 
ATGGAAAAGT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Molecular insights of organochlorine biocide-induced toxicity in zebrafish: Whole-adult-organism toxicogenomics, targeted gene expression and histological analyses.
Journal of genetics and genomics = Yi chuan xue bao43(8)525-8(2016 Aug)
Lui EY,Li C,Li ZH,Gong Z,Lam SH


book

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R