ORF cDNA Clones and Custom Clones » Species Summary » Danio rerio » spc24 cDNA ORF clone

spc24 cDNA ORF clone, Danio rerio(zebrafish)

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Summary

Gene Symbol	spc24
Entrez Gene ID	554147
Full Name	SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Synonyms	wu:fb79a02,zgc:110417
General protein information	Preferred Names SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) Names kinetochore protein Spc24
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017354329.1	XP_017209818.1	kinetochore protein Spc24 isoform X1
NM_001024440.1	NP_001019611.1	kinetochore protein Spc24

Homologies

Homo sapiens (human)	SPC24	NP_872319.1
Pan troglodytes (chimpanzee)	SPC24	XP_001167473.1
Xenopus tropicalis (tropical clawed frog)	spc24	NP_989057.1
	SPC24	XP_001104117.1
Danio rerio (zebrafish)	spc24	NP_001019611.1
Mus musculus (house mouse)	Spc24	NP_080558.1
Rattus norvegicus (Norway rat)	Spc24	XP_003750496.2
Canis lupus familiaris (dog)	SPC24	XP_853911.1
Bos taurus (cattle)	SPC24	NP_001068859.1

Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Jia XE, Ma K, Xu T, Gao L, Wu S, Fu C, Zhang W, Wang Z, Liu K, Dong M, Jing C, Ren C, Dong Z, Chen Y, Jin Y, Huang Q, Chang X, Deng M, Li L, Luo L, Zhu J, Dang Y, Chang HC, Zon LI, Zhou Y, Chen S, Pan W
Cell research25(8)946-62(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following spc24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spc24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38568	NM_001024440.1 Latest version!	Danio rerio SPC24 component of NDC80 kinetochore complex (spc24), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$174.30 $249.00
ODa58052	XM_017354329.1 Latest version!	Danio rerio SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (spc24), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spc24 ( NM_001024440.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001019611.1 Danio rerio SPC24 component of NDC80 kinetochore complex (spc24), mRNA.

Price & Availability↑

CloneID	ODa38568
Clone ID Related Accession (Same CDS sequence)	NM_001024440.1
Accession Version	NM_001024440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	kinetochore protein Spc24
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095251.1. ##Evidence-Data-START## Transcript exon combination :: BC095251.1, BC076486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCATA GCATTTCGAT GCATGATGAT GACCTTGAGA AATTAGGAGA ATCTTTGAAT 
GAGATCATCA TGAGCAGTAA GGCAGAAAGT GTTCTTCAAG CAGCAAGGAA AAAAAAGCAG 
GAGTTCTTCG ACCACTACAT CAAGACTAAG AAGACGCTTA CTGAGCTCCT AAATGATCTT 
GTGCACACAG AAGGGGTGGT CGGCCAGAAG CTTCTGGATT TAGACCAACA GAAAAGCCAG 
ATGATGCAGG AGATCATGAA GATGGAGCAG GAGGTGCAGA GGAGCCAGTC TAAAAGTCAA 
AACCTGGACT CAGAGCTGGA ATTCTTGCAG AAAGAGCTGG AAAGACTCCG AGATGCTGAG 
CAAGAAATCC AAACCCTGCA ACAGGAAGTG GATGAGGACA CAACTGAGGT CATTCCTTCA 
GCCATATATG TTGCTCAGCT CTACTCTAAA GTGACCAAGA TAAAGCTGGA GTTTGACACA 
GAGCCGCACA TACTGAAAGG AGTTCACTAT GGTGCGGATG TGGTGACCCC CATCAACATA 
GACACCTCCA CCCGTACACC CTGTGAGGTC AGCGATGAGC TCTGGAGCCT GGTTAAGACA 
GAATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019611.1
CDS	53..661
Translation	MSHSISMHDDDLEKLGESLNEIIMSSKAESVLQAARKKKQEFFDHYIKTKKTLTELLNDLVHTEGVVGQKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVDEDTTEVIPSAIYVAQLYSKVTKIKLEFDTEPHILKGVHYGADVVTPINIDTSTRTPCEVSDELWSLVKTEW

Target ORF information:

RefSeq Version	NM_001024440.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPC24 component of NDC80 kinetochore complex (spc24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024440.1

ORF Insert Sequence:

ATGTCCCATA GCATTTCGAT GCATGATGAT GACCTTGAGA AATTAGGAGA ATCTTTGAAT 
GAGATCATCA TGAGCAGTAA GGCAGAAAGT GTTCTTCAAG CAGCAAGGAA AAAAAAGCAG 
GAGTTCTTCG ACCACTACAT CAAGACTAAG AAGACGCTTA CTGAGCTCCT AAATGATCTT 
GTGCACACAG AAGGGGTGGT CGGCCAGAAG CTTCTGGATT TAGACCAACA GAAAAGCCAG 
ATGATGCAGG AGATCATGAA GATGGAGCAG GAGGTGCAGA GGAGCCAGTC TAAAAGTCAA 
AACCTGGACT CAGAGCTGGA ATTCTTGCAG AAAGAGCTGG AAAGACTCCG AGATGCTGAG 
CAAGAAATCC AAACCCTGCA ACAGGAAGTG GATGAGGACA CAACTGAGGT CATTCCTTCA 
GCCATATATG TTGCTCAGCT CTACTCTAAA GTGACCAAGA TAAAGCTGGA GTTTGACACA 
GAGCCGCACA TACTGAAAGG AGTTCACTAT GGTGCGGATG TGGTGACCCC CATCAACATA 
GACACCTCCA CCCGTACACC CTGTGAGGTC AGCGATGAGC TCTGGAGCCT GGTTAAGACA 
GAATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spc24 ( XM_017354329.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209818.1 Danio rerio SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (spc24), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58052
Clone ID Related Accession (Same CDS sequence)	XM_017354329.1
Accession Version	XM_017354329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinetochore protein Spc24 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCATA GCATTTCGAT GCATGATGAT GACCTTGAGA AATTAGGAGA ATCTTTGAAT 
GAGATCATCA TGAGCAGTAA GGCAGAAAGT GTTCTTCAAG CAGCAAGGAA AAAAAAGCAG 
GAGTTCTTCG ACCACTACAT CAAGACTAAG AAGACGCTTA CTGAGCTCCT AAATGATCTT 
GTGCACACAG AAGAGGTGGT CGGCCAGAAG CTTCTGGATT TAGACCAACA GAAAAGCCAG 
ATGATGCAGG AGATCATGAA GATGGAGCAG GAGGTGCAGA GGAGCCAGTC TAAAAGTCAA 
AACCTGGACT CAGAGCTGGA ATTCTTGCAG AAAGAGCTGG AAAGACTCCG AGATGCTGAG 
CAAGAAATCC AAACCCTGCA ACAGGAAGTG GATGAGGACA CAACTGAGGT CATTCCTTCA 
GCCATATATG TTGCTCAGCT CTACTCTAAA GTGACCAAGA TAAAGCTGGA GTTTGACACA 
GAGCCGCACA TACTGAAAGG AGTTCACTAT GGTGCGGATG TGGTGACCCC CATCAACATA 
GACACCTCCA CCCGTACACC CTGTGAGGTC AGCGATGAGC TCTGGAGCCT GGTTAAGACA 
GAATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209818.1
CDS	40..648
Translation	MSHSISMHDDDLEKLGESLNEIIMSSKAESVLQAARKKKQEFFDHYIKTKKTLTELLNDLVHTEEVVGQKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVDEDTTEVIPSAIYVAQLYSKVTKIKLEFDTEPHILKGVHYGADVVTPINIDTSTRTPCEVSDELWSLVKTEW

Target ORF information:

RefSeq Version	XM_017354329.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (spc24), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354329.1

ORF Insert Sequence:

ATGTCCCATA GCATTTCGAT GCATGATGAT GACCTTGAGA AATTAGGAGA ATCTTTGAAT 
GAGATCATCA TGAGCAGTAA GGCAGAAAGT GTTCTTCAAG CAGCAAGGAA AAAAAAGCAG 
GAGTTCTTCG ACCACTACAT CAAGACTAAG AAGACGCTTA CTGAGCTCCT AAATGATCTT 
GTGCACACAG AAGAGGTGGT CGGCCAGAAG CTTCTGGATT TAGACCAACA GAAAAGCCAG 
ATGATGCAGG AGATCATGAA GATGGAGCAG GAGGTGCAGA GGAGCCAGTC TAAAAGTCAA 
AACCTGGACT CAGAGCTGGA ATTCTTGCAG AAAGAGCTGG AAAGACTCCG AGATGCTGAG 
CAAGAAATCC AAACCCTGCA ACAGGAAGTG GATGAGGACA CAACTGAGGT CATTCCTTCA 
GCCATATATG TTGCTCAGCT CTACTCTAAA GTGACCAAGA TAAAGCTGGA GTTTGACACA 
GAGCCGCACA TACTGAAAGG AGTTCACTAT GGTGCGGATG TGGTGACCCC CATCAACATA 
GACACCTCCA CCCGTACACC CTGTGAGGTC AGCGATGAGC TCTGGAGCCT GGTTAAGACA 
GAATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Cell research25(8)946-62(2015 Aug)
Jia XE,Ma K,Xu T,Gao L,Wu S,Fu C,Zhang W,Wang Z,Liu K,Dong M,Jing C,Ren C,Dong Z,Chen Y,Jin Y,Huang Q,Chang X,Deng M,Li L,Luo L,Zhu J,Dang Y,Chang HC,Zon LI,Zhou Y,Chen S,Pan W

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spc24 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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