dhx34 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhx34
Entrez Gene ID	555838
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 34
Synonyms	si:ch211-255l12.1
General protein information	Preferred Names DEAH (Asp-Glu-Ala-His) box polypeptide 34 Names probable ATP-dependent RNA helicase DHX34
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351927.1	XP_017207416.1	probable ATP-dependent RNA helicase DHX34 isoform X1
XM_017351927.2	XP_017207416.1	probable ATP-dependent RNA helicase DHX34 isoform X1
NM_001128333.1	NP_001121805.1	probable ATP-dependent RNA helicase DHX34

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DHX34	XP_005616451.1
Caenorhabditis elegans (roundworm)	smgl-2	NP_500405.3
Homo sapiens (human)	DHX34	NP_055496.2
	LOC718100	XP_002801360.1
Bos taurus (cattle)	DHX34	NP_001179053.1
Drosophila melanogaster (fruit fly)	CG32533	NP_728244.1
Pan troglodytes (chimpanzee)	DHX34	XP_001169079.1
Mus musculus (house mouse)	Dhx34	NP_001272860.1
Rattus norvegicus (Norway rat)	Dhx34	NP_001258381.1
Danio rerio (zebrafish)	dhx34	NP_001121805.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001338	XP_321806.4
Xenopus tropicalis (tropical clawed frog)	dhx34	NP_001121459.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.
Longman D, Hug N, Keith M, Anastasaki C, Patton EE, Grimes G, C?ceres JF
Nucleic acids research41(17)8319-31(2013 Sep)

Dhx34 and Nbas function in the NMD pathway and are required for embryonic development in zebrafish.
Anastasaki C, Longman D, Capper A, Patton EE, C?ceres JF
Nucleic acids research39(9)3686-94(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DHX34 and NBAS act in concert with core nonsense-mediated mRNA decay factors to co-regulate a large number of endogenous RNA targets.
Title: DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.

Dhx34 is required for degradation of premature termination codons-containing mRNAs in zebrafish embryos.
Title: Dhx34 and Nbas function in the NMD pathway and are required for embryonic development in zebrafish.

The following dhx34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhx34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55802	XM_017351927.2 Latest version!	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa03953	NM_001128333.1 Latest version!	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa55802	XM_017351927.1	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhx34 ( XM_017351927.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207416.1 Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55802
Clone ID Related Accession (Same CDS sequence)	XM_017351927.1 , XM_017351927.2
Accession Version	XM_017351927.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent RNA helicase DHX34 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGTACTG ATAAAGGCCA GAGCAACAGG 
CATCAGTTGC TAGCATTCGT CACTCTCCTG GAAACAAACA AGCCTTACCT TTCAAACTGT 
GTGCGGGTCC CAGCCCTGCA GGCTCTGCTG CTGGTGGCCA ACTCGGTGAA CAGTAATGCG 
GACTGCACGC GGTTGGTGGT GGACGGCTGG CTGGAACTGA GCTTGACTGA TGGAGACGCG 
GCTCTAAAGG TGCTCTCCTC GGCCCTCAGT CTGCGGGCGG AATGGGAAAG GCTGCTTCAG 
GCTCAGCTCA GACAGAGCGG GTCTTGTTCC TCTGCTGGAT TCGGCTCAAG TGGACCAAGA 
GTGTCCCGCA GAGAACTAGA GAAGCTCTGT GAGGGACTTG TGCGGTTTCT GCTGTACACT 
GAGGTTAATT ATAGTTTGAA GCGTCTAACG GCGCTGCAGT CACAGAACCT GTATATCGGA 
CCTCAGCAGG ATCCGGATCT TTCTTCAGCT GCAGCTATCG GTTCTCTGTT TCCTGGGATG 
AAGGCAGAAC CAGATCCCAT TAAAGGAGGC CTGCGTTTGT GCAGCTACTT CACATACAAC 
TGTCTCTGTG ACTCTCAGGA CTTGTACAGT GAGTGTTTGC GCAAGTTTTG GAGTTGCCCT 
CGCTGTGACT TTTACATGCC TCTCACACCT CTGGAGCGCA TGCAGCACGA GGCCTCCTGC 
AGGCCGGCAG AGGAACAGCA GTCCGAGGAC GAGTCTGAAA GTGTAAATCC GTCCTCGTCG 
TCTTCATCTG CTCTGACCAG GGTTTACCAC TGTGATATCT GTAATAAAGA CCTGACTCTC 
ACCTCAACTG AGATCCTGAA ACACAAACGG CAGCATCTGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207416.1
CDS	196..3660
Translation	MSRKDEESRSWDWDSPRCRAQLDEVFFRQHDYIQAGSAEHKEFWSFFERFQSFKAKKEMTASKSSRQEKHVALREGKAKKKTGCVDLGLPKDYDARYRINISVCSRDIEERLAGSDHRRRESSGPGRQEITDGRLALLHFLDFNQKQSFSKLSKLRREQKSLPIFQYRDKIVELVRTHPVVVVAGDTGCGKSTQVPQYLLASGINNIACTQPRRIACISLAKRVSFESLNQYGSKVGYQIRFETSRTLATKLLFLTEGLLLRQIQQDASLGQYQVLIVDEVHERHLHCDFLLGVLRSLLSLRPDLRLVLMSATINIKLFSSYFNDAPVLQVPGRLFPIQVIYQPIPPEEQVSRSEKLDPRPYLRVLQGIDQRYPQEERGDLLLFLSGVAEISTIQEACQTYATHTQRWIVLPLHSTLSLAQQDKVFDIAPPGVRKCIISTNIAETSVTIDGVRFVVDSGKVKEMSFDPKAKMQRLQEFWISRASSEQRKGRAGRTGPGVCYRLYSESDYDAFAPYPVPEIHRVALDSLILQMKSMGLGDPRTFTFIDPPPASSIQTAVTYLREQGALDEHGELTSIGRLLAQLPVDVVIGKMLVLGSVFNLVEPVLTVAAALSVQSPFLRSAQHNPDCSTARQPLNSNHGDPFTLMNTFNAWVQVKADRGSGSRKWCRRRGLEEQRLYEMVNLRRQFKELLRSHGLLEDDTGRATEPQDRARRRERFTERQKLHQLKRDHELQENTKRKVLRLEEGLEGDASSGSDAEESSTSRKKRKDGAEQTMDIQEVKFKLRHNVNELQESAYASAALSSLQQALLKLLLCRGLYPQLAIPDEHNATRKDSDQVFHTRNKQGVVIHPTSVFASDPEVLHVPEHETSEFGTDKGQSNRHQLLAFVTLLETNKPYLSNCVRVPALQALLLVANSVNSNADCTRLVVDGWLELSLTDGDAALKVLSSALSLRAEWERLLQAQLRQSGSCSSAGFGSSGPRVSRRELEKLCEGLVRFLLYTEVNYSLKRLTALQSQNLYIGPQQDPDLSSAAAIGSLFPGMKAEPDPIKGGLRLCSYFTYNCLCDSQDLYSECLRKFWSCPRCDFYMPLTPLERMQHEASCRPAEEQQSEDESESVNPSSSSSSALTRVYHCDICNKDLTLTSTEILKHKRQHLS

Target ORF information:

RefSeq Version	XM_017351927.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351927.1

ORF Insert Sequence:

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGTACTG ATAAAGGCCA GAGCAACAGG 
CATCAGTTGC TAGCATTCGT CACTCTCCTG GAAACAAACA AGCCTTACCT TTCAAACTGT 
GTGCGGGTCC CAGCCCTGCA GGCTCTGCTG CTGGTGGCCA ACTCGGTGAA CAGTAATGCG 
GACTGCACGC GGTTGGTGGT GGACGGCTGG CTGGAACTGA GCTTGACTGA TGGAGACGCG 
GCTCTAAAGG TGCTCTCCTC GGCCCTCAGT CTGCGGGCGG AATGGGAAAG GCTGCTTCAG 
GCTCAGCTCA GACAGAGCGG GTCTTGTTCC TCTGCTGGAT TCGGCTCAAG TGGACCAAGA 
GTGTCCCGCA GAGAACTAGA GAAGCTCTGT GAGGGACTTG TGCGGTTTCT GCTGTACACT 
GAGGTTAATT ATAGTTTGAA GCGTCTAACG GCGCTGCAGT CACAGAACCT GTATATCGGA 
CCTCAGCAGG ATCCGGATCT TTCTTCAGCT GCAGCTATCG GTTCTCTGTT TCCTGGGATG 
AAGGCAGAAC CAGATCCCAT TAAAGGAGGC CTGCGTTTGT GCAGCTACTT CACATACAAC 
TGTCTCTGTG ACTCTCAGGA CTTGTACAGT GAGTGTTTGC GCAAGTTTTG GAGTTGCCCT 
CGCTGTGACT TTTACATGCC TCTCACACCT CTGGAGCGCA TGCAGCACGA GGCCTCCTGC 
AGGCCGGCAG AGGAACAGCA GTCCGAGGAC GAGTCTGAAA GTGTAAATCC GTCCTCGTCG 
TCTTCATCTG CTCTGACCAG GGTTTACCAC TGTGATATCT GTAATAAAGA CCTGACTCTC 
ACCTCAACTG AGATCCTGAA ACACAAACGG CAGCATCTGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx34 ( XM_017351927.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207416.1 Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55802
Clone ID Related Accession (Same CDS sequence)	XM_017351927.1 , XM_017351927.2
Accession Version	XM_017351927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DHX34 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGTACTG ATAAAGGCCA GAGCAACAGG 
CATCAGTTGC TAGCATTCGT CACTCTCCTG GAAACAAACA AGCCTTACCT TTCAAACTGT 
GTGCGGGTCC CAGCCCTGCA GGCTCTGCTG CTGGTGGCCA ACTCGGTGAA CAGTAATGCG 
GACTGCACGC GGTTGGTGGT GGACGGCTGG CTGGAACTGA GCTTGACTGA TGGAGACGCG 
GCTCTAAAGG TGCTCTCCTC GGCCCTCAGT CTGCGGGCGG AATGGGAAAG GCTGCTTCAG 
GCTCAGCTCA GACAGAGCGG GTCTTGTTCC TCTGCTGGAT TCGGCTCAAG TGGACCAAGA 
GTGTCCCGCA GAGAACTAGA GAAGCTCTGT GAGGGACTTG TGCGGTTTCT GCTGTACACT 
GAGGTTAATT ATAGTTTGAA GCGTCTAACG GCGCTGCAGT CACAGAACCT GTATATCGGA 
CCTCAGCAGG ATCCGGATCT TTCTTCAGCT GCAGCTATCG GTTCTCTGTT TCCTGGGATG 
AAGGCAGAAC CAGATCCCAT TAAAGGAGGC CTGCGTTTGT GCAGCTACTT CACATACAAC 
TGTCTCTGTG ACTCTCAGGA CTTGTACAGT GAGTGTTTGC GCAAGTTTTG GAGTTGCCCT 
CGCTGTGACT TTTACATGCC TCTCACACCT CTGGAGCGCA TGCAGCACGA GGCCTCCTGC 
AGGCCGGCAG AGGAACAGCA GTCCGAGGAC GAGTCTGAAA GTGTAAATCC GTCCTCGTCG 
TCTTCATCTG CTCTGACCAG GGTTTACCAC TGTGATATCT GTAATAAAGA CCTGACTCTC 
ACCTCAACTG AGATCCTGAA ACACAAACGG CAGCATCTGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207416.1
CDS	196..3660
Translation	MSRKDEESRSWDWDSPRCRAQLDEVFFRQHDYIQAGSAEHKEFWSFFERFQSFKAKKEMTASKSSRQEKHVALREGKAKKKTGCVDLGLPKDYDARYRINISVCSRDIEERLAGSDHRRRESSGPGRQEITDGRLALLHFLDFNQKQSFSKLSKLRREQKSLPIFQYRDKIVELVRTHPVVVVAGDTGCGKSTQVPQYLLASGINNIACTQPRRIACISLAKRVSFESLNQYGSKVGYQIRFETSRTLATKLLFLTEGLLLRQIQQDASLGQYQVLIVDEVHERHLHCDFLLGVLRSLLSLRPDLRLVLMSATINIKLFSSYFNDAPVLQVPGRLFPIQVIYQPIPPEEQVSRSEKLDPRPYLRVLQGIDQRYPQEERGDLLLFLSGVAEISTIQEACQTYATHTQRWIVLPLHSTLSLAQQDKVFDIAPPGVRKCIISTNIAETSVTIDGVRFVVDSGKVKEMSFDPKAKMQRLQEFWISRASSEQRKGRAGRTGPGVCYRLYSESDYDAFAPYPVPEIHRVALDSLILQMKSMGLGDPRTFTFIDPPPASSIQTAVTYLREQGALDEHGELTSIGRLLAQLPVDVVIGKMLVLGSVFNLVEPVLTVAAALSVQSPFLRSAQHNPDCSTARQPLNSNHGDPFTLMNTFNAWVQVKADRGSGSRKWCRRRGLEEQRLYEMVNLRRQFKELLRSHGLLEDDTGRATEPQDRARRRERFTERQKLHQLKRDHELQENTKRKVLRLEEGLEGDASSGSDAEESSTSRKKRKDGAEQTMDIQEVKFKLRHNVNELQESAYASAALSSLQQALLKLLLCRGLYPQLAIPDEHNATRKDSDQVFHTRNKQGVVIHPTSVFASDPEVLHVPEHETSEFGTDKGQSNRHQLLAFVTLLETNKPYLSNCVRVPALQALLLVANSVNSNADCTRLVVDGWLELSLTDGDAALKVLSSALSLRAEWERLLQAQLRQSGSCSSAGFGSSGPRVSRRELEKLCEGLVRFLLYTEVNYSLKRLTALQSQNLYIGPQQDPDLSSAAAIGSLFPGMKAEPDPIKGGLRLCSYFTYNCLCDSQDLYSECLRKFWSCPRCDFYMPLTPLERMQHEASCRPAEEQQSEDESESVNPSSSSSSALTRVYHCDICNKDLTLTSTEILKHKRQHLS

Target ORF information:

RefSeq Version	XM_017351927.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351927.2

ORF Insert Sequence:

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGTACTG ATAAAGGCCA GAGCAACAGG 
CATCAGTTGC TAGCATTCGT CACTCTCCTG GAAACAAACA AGCCTTACCT TTCAAACTGT 
GTGCGGGTCC CAGCCCTGCA GGCTCTGCTG CTGGTGGCCA ACTCGGTGAA CAGTAATGCG 
GACTGCACGC GGTTGGTGGT GGACGGCTGG CTGGAACTGA GCTTGACTGA TGGAGACGCG 
GCTCTAAAGG TGCTCTCCTC GGCCCTCAGT CTGCGGGCGG AATGGGAAAG GCTGCTTCAG 
GCTCAGCTCA GACAGAGCGG GTCTTGTTCC TCTGCTGGAT TCGGCTCAAG TGGACCAAGA 
GTGTCCCGCA GAGAACTAGA GAAGCTCTGT GAGGGACTTG TGCGGTTTCT GCTGTACACT 
GAGGTTAATT ATAGTTTGAA GCGTCTAACG GCGCTGCAGT CACAGAACCT GTATATCGGA 
CCTCAGCAGG ATCCGGATCT TTCTTCAGCT GCAGCTATCG GTTCTCTGTT TCCTGGGATG 
AAGGCAGAAC CAGATCCCAT TAAAGGAGGC CTGCGTTTGT GCAGCTACTT CACATACAAC 
TGTCTCTGTG ACTCTCAGGA CTTGTACAGT GAGTGTTTGC GCAAGTTTTG GAGTTGCCCT 
CGCTGTGACT TTTACATGCC TCTCACACCT CTGGAGCGCA TGCAGCACGA GGCCTCCTGC 
AGGCCGGCAG AGGAACAGCA GTCCGAGGAC GAGTCTGAAA GTGTAAATCC GTCCTCGTCG 
TCTTCATCTG CTCTGACCAG GGTTTACCAC TGTGATATCT GTAATAAAGA CCTGACTCTC 
ACCTCAACTG AGATCCTGAA ACACAAACGG CAGCATCTGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx34 ( NM_001128333.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121805.1 Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), mRNA.

Price & Availability↑

CloneID	ODa03953
Clone ID Related Accession (Same CDS sequence)	NM_001128333.1
Accession Version	NM_001128333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DHX34
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX908775.18. On Jul 31, 2008 this sequence version replaced XM_678482.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGCCAGA GCAACAGGCA TCAGTTGCTA 
GCATTCGTCA CTCTCCTGGA AACAAACAAG CCTTACCTTT CAAACTGTGT GCGGGTCCCA 
GCCCTGCAGG CTCTGCTGCT GGTGGCCAAC TCGGTGAACA GTAATGCGGA CTGCACGCGG 
TTGGTGGTGG ACGGCTGGCT GGAACTGAGC TTGACTGATG GAGACGCGGC TCTAAAGGTG 
CTCTCCTCGG CCCTCAGTCT GCGGGCGGAA TGGGAAAGGC TGCTTCAGGC TCAGCTCAGA 
CAGAGCGGGT CTTGTTCCTC TGCTGGATTC GGCTCAAGTG GACCAAGAGT GTCCCGCAGA 
GAACTAGAGA AGCTCTGTGA GGGACTTGTG CGGTTTCTGC TGTACACTGA GGTTAATTAT 
AGTTTGAAGC GTCTAACGGC GCTGCAGTCA CAGAACCTGT ATATCGGACC TCAGCAGGAT 
CCGGATCTTT CTTCAGCTGC AGCTATCGGT TCTCTGTTTC CTGGGATGAA GGCAGAACCA 
GATCCCATTA AAGGAGGCCT GCGTTTGTGC AGCTACTTCA CATACAACTG TCTCTGTGAC 
TCTCAGGACT TGTACAGTGA GTGTTTGCGC AAGTTTTGGA GTTGCCCTCG CTGTGACTTT 
TACATGCCTC TCACACCTCT GGAGCGCATG CAGCACGAGG CCTCCTGCAG GCCGGCAGAG 
GAACAGCAGT CCGAGGACGA GTCTGAAAGT GTAAATCCGT CCTCGTCGTC TTCATCTGCT 
CTGACCAGGG TTTACCACTG TGATATCTGT AATAAAGACC TGACTCTCAC CTCAACTGAG 
ATCCTGAAAC ACAAACGGCA GCATCTGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121805.1
CDS	196..3648
Translation	MSRKDEESRSWDWDSPRCRAQLDEVFFRQHDYIQAGSAEHKEFWSFFERFQSFKAKKEMTASKSSRQEKHVALREGKAKKKTGCVDLGLPKDYDARYRINISVCSRDIEERLAGSDHRRRESSGPGRQEITDGRLALLHFLDFNQKQSFSKLSKLRREQKSLPIFQYRDKIVELVRTHPVVVVAGDTGCGKSTQVPQYLLASGINNIACTQPRRIACISLAKRVSFESLNQYGSKVGYQIRFETSRTLATKLLFLTEGLLLRQIQQDASLGQYQVLIVDEVHERHLHCDFLLGVLRSLLSLRPDLRLVLMSATINIKLFSSYFNDAPVLQVPGRLFPIQVIYQPIPPEEQVSRSEKLDPRPYLRVLQGIDQRYPQEERGDLLLFLSGVAEISTIQEACQTYATHTQRWIVLPLHSTLSLAQQDKVFDIAPPGVRKCIISTNIAETSVTIDGVRFVVDSGKVKEMSFDPKAKMQRLQEFWISRASSEQRKGRAGRTGPGVCYRLYSESDYDAFAPYPVPEIHRVALDSLILQMKSMGLGDPRTFTFIDPPPASSIQTAVTYLREQGALDEHGELTSIGRLLAQLPVDVVIGKMLVLGSVFNLVEPVLTVAAALSVQSPFLRSAQHNPDCSTARQPLNSNHGDPFTLMNTFNAWVQVKADRGSGSRKWCRRRGLEEQRLYEMVNLRRQFKELLRSHGLLEDDTGRATEPQDRARRRERFTERQKLHQLKRDHELQENTKRKVLRLEEGLEGDASSGSDAEESSTSRKKRKDGAEQTMDIQEVKFKLRHNVNELQESAYASAALSSLQQALLKLLLCRGLYPQLAIPDEHNATRKDSDQVFHTRNKQGVVIHPTSVFASDPEVLHVPEHETSEFGQSNRHQLLAFVTLLETNKPYLSNCVRVPALQALLLVANSVNSNADCTRLVVDGWLELSLTDGDAALKVLSSALSLRAEWERLLQAQLRQSGSCSSAGFGSSGPRVSRRELEKLCEGLVRFLLYTEVNYSLKRLTALQSQNLYIGPQQDPDLSSAAAIGSLFPGMKAEPDPIKGGLRLCSYFTYNCLCDSQDLYSECLRKFWSCPRCDFYMPLTPLERMQHEASCRPAEEQQSEDESESVNPSSSSSSALTRVYHCDICNKDLTLTSTEILKHKRQHLS

Target ORF information:

RefSeq Version	NM_001128333.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 34 (dhx34), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128333.1

ORF Insert Sequence:

ATGTCCCGTA AGGATGAAGA GAGTCGGAGC TGGGACTGGG ACAGCCCTCG CTGCCGAGCC 
CAGCTGGATG AGGTGTTTTT CCGCCAGCAT GACTACATCC AGGCGGGCAG CGCCGAACAC 
AAGGAGTTCT GGAGCTTCTT CGAGCGGTTC CAGAGTTTTA AAGCCAAGAA AGAGATGACG 
GCCTCCAAAT CCAGCAGACA AGAAAAACAC GTCGCTCTGC GTGAGGGGAA AGCCAAAAAG 
AAGACTGGAT GTGTGGATCT GGGCCTTCCA AAAGACTACG ATGCCAGATA CCGGATCAAC 
ATCTCGGTGT GCTCCAGAGA CATAGAGGAG CGGCTGGCAG GATCAGACCA TCGGCGGAGA 
GAGAGCTCGG GTCCCGGCAG ACAGGAGATC ACAGACGGCC GCCTGGCTCT TTTACATTTC 
CTAGACTTTA ACCAGAAACA GAGCTTCAGC AAACTTTCTA AGCTGAGAAG AGAGCAGAAG 
AGCCTGCCCA TCTTCCAGTA CCGGGACAAG ATCGTGGAGC TGGTGAGGAC TCACCCTGTG 
GTGGTGGTGG CTGGAGACAC CGGATGTGGA AAGTCCACGC AGGTGCCCCA GTATCTGCTG 
GCGTCAGGAA TCAACAACAT CGCCTGCACT CAACCACGCC GCATCGCCTG CATCTCACTG 
GCCAAGAGAG TCAGCTTCGA GAGCCTCAAC CAGTACGGCT CCAAGGTCGG GTACCAGATC 
CGCTTTGAGA CGAGCCGCAC CCTAGCCACC AAACTGCTGT TCCTCACCGA GGGCCTGCTG 
CTCAGGCAGA TCCAGCAGGA CGCGTCGCTG GGCCAGTACC AGGTGCTGAT CGTAGACGAG 
GTGCACGAGC GACACCTCCA CTGTGACTTC CTACTGGGAG TTCTGCGCTC CCTGTTGTCT 
CTGCGTCCTG ACCTGCGGCT CGTGCTCATG TCGGCCACCA TCAACATCAA GCTCTTCTCC 
AGCTACTTCA ATGACGCTCC TGTGCTGCAG GTGCCAGGCA GACTCTTCCC CATTCAGGTA 
ATCTACCAGC CTATTCCTCC AGAAGAGCAG GTGTCACGCT CTGAGAAGCT GGACCCGCGG 
CCGTACCTGC GTGTTCTGCA GGGGATTGAC CAGCGCTACC CGCAGGAGGA GAGAGGAGAC 
CTGCTGCTGT TCCTCAGCGG AGTGGCTGAG ATCTCCACCA TCCAGGAGGC CTGCCAGACG 
TACGCCACAC ACACACAGCG CTGGATCGTC CTCCCTCTGC ACAGCACACT CTCGCTGGCA 
CAGCAGGATA AGGTGTTTGA CATTGCACCT CCAGGGGTAA GAAAATGCAT AATTTCCACC 
AATATTGCAG AGACCTCAGT CACCATCGAT GGAGTTCGCT TTGTGGTTGA TTCAGGAAAA 
GTGAAAGAGA TGAGTTTTGA CCCCAAGGCC AAGATGCAGA GGCTGCAGGA GTTCTGGATC 
AGCAGGGCCA GTTCCGAACA GAGGAAAGGT CGTGCGGGCA GAACTGGACC GGGGGTCTGT 
TACCGACTCT ACTCTGAATC TGATTACGAT GCCTTTGCAC CATATCCCGT CCCTGAGATC 
CACAGGGTGG CGCTGGACTC GCTCATCCTA CAGATGAAGA GTATGGGTCT GGGGGACCCT 
CGCACCTTTA CCTTCATCGA CCCTCCTCCT GCCTCCAGTA TTCAGACTGC AGTGACGTAC 
CTGAGAGAAC AGGGGGCGCT GGATGAACAC GGGGAGCTCA CTTCCATTGG CAGACTGCTG 
GCTCAACTGC CTGTGGATGT GGTCATTGGT AAAATGTTAG TTTTGGGTTC TGTCTTCAAC 
CTGGTGGAGC CGGTGCTGAC GGTGGCCGCT GCTCTGAGTG TCCAGTCTCC ATTCCTGCGC 
AGTGCTCAAC ACAACCCAGA CTGCTCCACA GCTCGCCAGC CTCTCAACAG CAACCACGGA 
GACCCGTTTA CTCTCATGAA CACCTTTAAC GCCTGGGTGC AGGTAAAAGC AGACAGAGGA 
AGTGGCTCCA GAAAGTGGTG CAGACGGAGA GGTCTGGAGG AACAGAGGCT TTACGAAATG 
GTGAACTTGA GAAGGCAATT CAAAGAGCTG CTGCGCTCTC ATGGTCTTCT GGAGGATGAT 
ACGGGCAGAG CAACCGAACC GCAGGACCGA GCTCGACGCC GGGAGAGATT CACTGAGAGA 
CAGAAGCTCC ACCAGCTGAA GCGAGACCAC GAGCTCCAGG AAAACACCAA GAGGAAAGTG 
CTGCGGCTGG AGGAGGGACT AGAAGGAGAC GCTTCATCTG GGTCTGATGC GGAGGAATCA 
AGCACAAGCA GGAAAAAAAG AAAAGATGGT GCTGAACAGA CTATGGATAT ACAGGAAGTC 
AAGTTCAAGT TGCGTCACAA TGTGAATGAG CTGCAGGAGT CTGCATATGC CAGTGCTGCT 
CTGTCGTCAT TGCAGCAGGC TCTGCTCAAA CTGCTGCTGT GTCGGGGTCT GTACCCACAG 
CTCGCTATTC CAGATGAGCA CAACGCTACG CGCAAAGACT CTGATCAGGT ATTCCACACA 
CGAAATAAAC AAGGTGTTGT GATCCACCCG ACCAGTGTGT TTGCCAGTGA TCCTGAAGTC 
CTGCACGTCC CTGAACATGA GACCAGCGAA TTCGGCCAGA GCAACAGGCA TCAGTTGCTA 
GCATTCGTCA CTCTCCTGGA AACAAACAAG CCTTACCTTT CAAACTGTGT GCGGGTCCCA 
GCCCTGCAGG CTCTGCTGCT GGTGGCCAAC TCGGTGAACA GTAATGCGGA CTGCACGCGG 
TTGGTGGTGG ACGGCTGGCT GGAACTGAGC TTGACTGATG GAGACGCGGC TCTAAAGGTG 
CTCTCCTCGG CCCTCAGTCT GCGGGCGGAA TGGGAAAGGC TGCTTCAGGC TCAGCTCAGA 
CAGAGCGGGT CTTGTTCCTC TGCTGGATTC GGCTCAAGTG GACCAAGAGT GTCCCGCAGA 
GAACTAGAGA AGCTCTGTGA GGGACTTGTG CGGTTTCTGC TGTACACTGA GGTTAATTAT 
AGTTTGAAGC GTCTAACGGC GCTGCAGTCA CAGAACCTGT ATATCGGACC TCAGCAGGAT 
CCGGATCTTT CTTCAGCTGC AGCTATCGGT TCTCTGTTTC CTGGGATGAA GGCAGAACCA 
GATCCCATTA AAGGAGGCCT GCGTTTGTGC AGCTACTTCA CATACAACTG TCTCTGTGAC 
TCTCAGGACT TGTACAGTGA GTGTTTGCGC AAGTTTTGGA GTTGCCCTCG CTGTGACTTT 
TACATGCCTC TCACACCTCT GGAGCGCATG CAGCACGAGG CCTCCTGCAG GCCGGCAGAG 
GAACAGCAGT CCGAGGACGA GTCTGAAAGT GTAAATCCGT CCTCGTCGTC TTCATCTGCT 
CTGACCAGGG TTTACCACTG TGATATCTGT AATAAAGACC TGACTCTCAC CTCAACTGAG 
ATCCTGAAAC ACAAACGGCA GCATCTGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.
Nucleic acids research41(17)8319-31(2013 Sep)
Longman D,Hug N,Keith M,Anastasaki C,Patton EE,Grimes G,C?ceres JF

Dhx34 and Nbas function in the NMD pathway and are required for embryonic development in zebrafish.
Nucleic acids research39(9)3686-94(2011 May)
Anastasaki C,Longman D,Capper A,Patton EE,C?ceres JF

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

dhx34 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

FAX