raph1b cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following raph1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the raph1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa01678 NM_001128244.1
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa05820 NM_001365585.1
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05821 NM_001365587.1
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05822 NM_001365589.1
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05820 XM_005159954.4
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05820 XM_009291866.3
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05821 XM_005159955.4
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05822 XM_005159956.4
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05823 XM_005159957.4
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa43264 XM_009291873.3
Latest version!
Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa05821 XM_005159955.3 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05820 XM_009291866.2 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05820 XM_005159954.3 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa05823 XM_005159957.3 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa05822 XM_005159956.3 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43264 XM_009291873.2 Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa01678
    Clone ID Related Accession (Same CDS sequence) NM_001128244.1
    Accession Version NM_001128244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-03-01
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929045.7. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA708836, SAMEA898404 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGGTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCC
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TCAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TATGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCCCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTAC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATT
    CAGTCACAGC CTCTACCGCC TCCTCCACCC ATAACACCCA TGCAGTCACA TGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCAAT AATGCCCATG CAGTCAGAGC CTCTACCGCC GCCTGCACAA
    ATCATGCCCA TGCAATCACA TCCTCTTCCA CCACCTCCAC CTTTAGTGCA AATGCAGTCA
    CAGCCCATGC CACCACCTCC TCCCACAGTG TCATTCCAAT CACAGCCTTT ACCACCTCCT
    CCACCCCCTC TCCAGGCTAA TGGCCTTCCC CCTCCTCCAC CTTTAATCAC TAATGGGCTT
    AAGCAGGCTT TTGCCCATAA ATTTCCCAGC AGACCACAGG ATGTATTACC CCCAGCTAAT
    CAAAACAATT TTCCTCCACC TCCCCCACCA CCACCACCTC CACCACTTGC ACCTACGCTC
    CCTCCTTACC AGCATGTTAC TTCAACTTCA AATCCACCAC CACCCCCACC ACCACCACCT
    CTTCCCCCAC CAGCCCCCAT ACATCTACCA AATCAACCTG CGGTTTTACC CAAATTTTTC
    ACTGGTGGAT TTCCACCTCA GAAAGCACCC AAACCTTCAT GTGGCATTCT TCCAGTATCC
    CCAGTAGTTC CAGCACTTCA ACCTCCACCA CCGCCTCCTC CTCCACCTCC TCCTCCACCA
    CCTGCACCAT TGAAGAATCA GCCTCCACCT ACACTGCCCA AGCAGCACAG CCTCTCCAAG
    ACATTGCCCT TCTCCAGTCA AACTTCATCA GTACCGTCTA TGGTTAAGCA GCTTGCTAGT
    CAATTTCCTG TTGCATCTCC TGCATTAACC AATCAAGCAG ATAGTCTCAA AGCTCCCCAG
    TCTCCACCTG CAGTGAAGGT TAAGCCAAAA TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA
    CAGAGGTCAC CTGAATTTCC ACCACCTCCA CCAGACAATG CCATTGGCTT TGCTTCACCA
    ACACCTCCAC CACCACCACC TCCTCCTCTT CCTCCAGGTC CTACTCCTCC TCCTCCCCCT
    CCTCCTCCTC CTCTGATGTC AGGCTCCCCT ATCAAAAAGT CTCCCTCTGG CTCATCTGCA
    GGTGTTAAGA AACCACCGCC AACTCCTCAG CGCAACTCCA GTGTGAAGTA CGATGCCTCA
    ACGGAATACC AGGAGTCCCG GAAGAACTTG GTGAGCAAGT TTAACCCAAG TTCTGCTTCA
    TCAACAACAT CAACCTCCAG CTGTTCACCC TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA
    CCCCCCAAAC CAGGGAAGCT CAATCTGGCA AATCTTCCTT TGGCTTTGCA GAACAAATTC
    AATCAGAACC GCCAGTCAGC GGCAGACTTT CCATCTCCTC CACCCCCAGA AAATGATTTT
    TTCCCACCTC CGCCAATAGA GTCCGACCTC CCTCCACCAC CACCTCTTCC AGGGGATCTA
    AATGGTGTGT CTCCCAAAGT GGCCGTAGTC AATCCTCAGC CTCAGCCTGC TTGGGGCAAA
    AGTTCCCTAA AGAAGACACA ACCTCCAGCA TCACTTCGCT GTAATAACAC TGTTAGAGAG
    TCTCCACCAC TCTCACCACC TTCCATACAC GGGGGGCCAA TTCCCCATTC TCCCAAAGGC
    ACCACACAGC CAAACTTTTT GGAGGACCTT AACAAGACAC TGAAACGCAA GTCTTCAAGA
    GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC ACAATGGATG ACATGGTTTT GCCACCACCT
    CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC
    ATCTCAGGCT ATGCAACACT ACGGCGAGGG CCTCCTCCTG CCCCACCCAA GCGGGACCAC
    AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121716.1
    CDS252..4712
    Translation

    Target ORF information:

    RefSeq Version NM_001128244.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGGTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCC
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TCAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TATGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCCCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTAC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATT
    CAGTCACAGC CTCTACCGCC TCCTCCACCC ATAACACCCA TGCAGTCACA TGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCAAT AATGCCCATG CAGTCAGAGC CTCTACCGCC GCCTGCACAA
    ATCATGCCCA TGCAATCACA TCCTCTTCCA CCACCTCCAC CTTTAGTGCA AATGCAGTCA
    CAGCCCATGC CACCACCTCC TCCCACAGTG TCATTCCAAT CACAGCCTTT ACCACCTCCT
    CCACCCCCTC TCCAGGCTAA TGGCCTTCCC CCTCCTCCAC CTTTAATCAC TAATGGGCTT
    AAGCAGGCTT TTGCCCATAA ATTTCCCAGC AGACCACAGG ATGTATTACC CCCAGCTAAT
    CAAAACAATT TTCCTCCACC TCCCCCACCA CCACCACCTC CACCACTTGC ACCTACGCTC
    CCTCCTTACC AGCATGTTAC TTCAACTTCA AATCCACCAC CACCCCCACC ACCACCACCT
    CTTCCCCCAC CAGCCCCCAT ACATCTACCA AATCAACCTG CGGTTTTACC CAAATTTTTC
    ACTGGTGGAT TTCCACCTCA GAAAGCACCC AAACCTTCAT GTGGCATTCT TCCAGTATCC
    CCAGTAGTTC CAGCACTTCA ACCTCCACCA CCGCCTCCTC CTCCACCTCC TCCTCCACCA
    CCTGCACCAT TGAAGAATCA GCCTCCACCT ACACTGCCCA AGCAGCACAG CCTCTCCAAG
    ACATTGCCCT TCTCCAGTCA AACTTCATCA GTACCGTCTA TGGTTAAGCA GCTTGCTAGT
    CAATTTCCTG TTGCATCTCC TGCATTAACC AATCAAGCAG ATAGTCTCAA AGCTCCCCAG
    TCTCCACCTG CAGTGAAGGT TAAGCCAAAA TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA
    CAGAGGTCAC CTGAATTTCC ACCACCTCCA CCAGACAATG CCATTGGCTT TGCTTCACCA
    ACACCTCCAC CACCACCACC TCCTCCTCTT CCTCCAGGTC CTACTCCTCC TCCTCCCCCT
    CCTCCTCCTC CTCTGATGTC AGGCTCCCCT ATCAAAAAGT CTCCCTCTGG CTCATCTGCA
    GGTGTTAAGA AACCACCGCC AACTCCTCAG CGCAACTCCA GTGTGAAGTA CGATGCCTCA
    ACGGAATACC AGGAGTCCCG GAAGAACTTG GTGAGCAAGT TTAACCCAAG TTCTGCTTCA
    TCAACAACAT CAACCTCCAG CTGTTCACCC TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA
    CCCCCCAAAC CAGGGAAGCT CAATCTGGCA AATCTTCCTT TGGCTTTGCA GAACAAATTC
    AATCAGAACC GCCAGTCAGC GGCAGACTTT CCATCTCCTC CACCCCCAGA AAATGATTTT
    TTCCCACCTC CGCCAATAGA GTCCGACCTC CCTCCACCAC CACCTCTTCC AGGGGATCTA
    AATGGTGTGT CTCCCAAAGT GGCCGTAGTC AATCCTCAGC CTCAGCCTGC TTGGGGCAAA
    AGTTCCCTAA AGAAGACACA ACCTCCAGCA TCACTTCGCT GTAATAACAC TGTTAGAGAG
    TCTCCACCAC TCTCACCACC TTCCATACAC GGGGGGCCAA TTCCCCATTC TCCCAAAGGC
    ACCACACAGC CAAACTTTTT GGAGGACCTT AACAAGACAC TGAAACGCAA GTCTTCAAGA
    GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC ACAATGGATG ACATGGTTTT GCCACCACCT
    CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC
    ATCTCAGGCT ATGCAACACT ACGGCGAGGG CCTCCTCCTG CCCCACCCAA GCGGGACCAC
    AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05820
    Clone ID Related Accession (Same CDS sequence) NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
    Accession Version NM_001365585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-21
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On Sep 12, 2018 this sequence version replaced XM_009291866.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898401 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352514.1
    CDS298..4995
    Translation

    Target ORF information:

    RefSeq Version NM_001365585.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05821
    Clone ID Related Accession (Same CDS sequence) XM_005159955.4 , XM_005159955.3 , NM_001365587.1
    Accession Version NM_001365587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-21
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On Sep 12, 2018 this sequence version replaced XM_005159955.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898401 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352516.1
    CDS298..4908
    Translation

    Target ORF information:

    RefSeq Version NM_001365587.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05822
    Clone ID Related Accession (Same CDS sequence) XM_005159956.3 , NM_001365589.1 , XM_005159956.4
    Accession Version NM_001365589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-21
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005426.17. On or before Sep 5, 2018 this sequence version replaced NM_001128244.1, XM_005159956.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898404 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352518.1
    CDS298..4833
    Translation

    Target ORF information:

    RefSeq Version NM_001365589.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05820
    Clone ID Related Accession (Same CDS sequence) NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
    Accession Version XM_005159954.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159954.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160011.1
    CDS312..5009
    Translation

    Target ORF information:

    RefSeq Version XM_005159954.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159954.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05820
    Clone ID Related Accession (Same CDS sequence) NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
    Accession Version XM_009291866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290141.1
    CDS309..5006
    Translation

    Target ORF information:

    RefSeq Version XM_009291866.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05820
    Clone ID Related Accession (Same CDS sequence) NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
    Accession Version XM_009291866.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290141.1
    CDS367..5064
    Translation

    Target ORF information:

    RefSeq Version XM_009291866.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05820
    Clone ID Related Accession (Same CDS sequence) NM_001365585.1 , XM_005159954.4 , XM_009291866.3 , XM_009291866.2 , XM_005159954.3
    Accession Version XM_005159954.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160011.1
    CDS370..5067
    Translation

    Target ORF information:

    RefSeq Version XM_005159954.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159954.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT CACTCGTATC TGGACAGAGA GACCTCTCTG
    ATTCTGAAAA ACATTGCAGG AAAACCCTCT CACCTGCTGA CCAAGGAAGA GCAGGCTGCC
    AAAGCGAAGG CTGAGAAAAT ACGTGTGGCT CTAGAGAAAA TTAAAGAAGC CCAAGTGAAA
    AAGCTGGTGA TACGTGTACA CATGTCAGAT GAGAGCTCAA AGACCATGAT GGTGGATGAG
    AGGCAAACGG TCAGGCAGTT GCTGGACAGT CTGCTGGATA AATCTCACTG CGGTTACAGT
    CCTGATTGGG CTCTGGTGGA GACCATCCCT GAGCTACAGA TGGAGAGAAT TTTTGAAGAT
    CACGAGAACC TTGTAGAGAA CTTGCTCAAC TGGACCAGAG ACAGTCAGAA CAAACTCATG
    TTCATTGAAC GGATTGAAAA GTATGCACTT TTCAAAAACC CTCAGAACTA TTTACTTGGG
    AGGAAGGAGA CTTCTGAGAT GGCAGACAGA AACAAGGAGG CACTGCTAGA GGAATGTTTT
    TGTGGCAGCT CGGTGTCAGT TCCTGAAATC GAAGGAGTGT TGTGGTTGAA GGAAGATGGT
    AAAAAGTCTT GGAAGAAGCG CTACTTTCTG CTAAGGGCAT CTGGCATCTA TTTTGTGCCA
    AAAGGCAAAG CCAAGGCTTC CAGGGATTTG GTGTGCTTCC TGCAGTTGGA CCATGTTAAC
    GTGTATTATG GTCAGGACTA CAGAAGCAAG TACAAGGCCC CCACTGACTA CTGCCTGGCC
    CTCAAGCACC CTCAGATCCA GAAAAAGTCA CAGTACATCA AATATCTATG CTGTGATGAT
    GTCAGAACTT TACACCAGTG GGTAAATGGC ATCCGTATTG CTAAGTACGG GAAGCAGTTG
    TATGTGAACT ACCAGGAAGC TATGAAGCGA ACAGAGGCTG CCTATGACTG GTCTTCTCTC
    TCCAGCACCA GCATCAAGTC AGGAACAAGC TCAGTCAGTA TACCTGAGTC GCAGTCAAAC
    CACTCCAGCC AGACAGACAG TGGTATGTCA GATGGTACTT CGTCCTCTCA TGCCCGCTCT
    CAAAGTGTGG TCAGTTCAAT CTTTTCTGAG GCCTGGAAGA GAGGAACTCA AATAGAGGAG
    AGCACTAAGG CAAGGCCAGA ATCAATCCGT TCTACTCATT CAAGCCATAG CAGTCATTCT
    TCTCATCACG TATCTCAACA GCAACATCTA CACGCACAAC AGCCACATGT GCATTCAATT
    CTACAATCAC GTGGAAGTGT CACCCAGGCT CCGGCACAGG CACCAACACA AACACTGGCA
    CAAGCACCAC CCCAAGTGGC TCCAGCTCCA CCACCTCCTC CTCCACCTCC TCCTCCACCA
    CCTCCTCCTC CTATTTCCAG CATCCCTCAC CATGCAACTC CTACCATGTT TGCCAAATAC
    AGCACTATCA CTCGGCTGCA AAATGCCTCT CAGGCTACAA GTCACCACAA GCCTCAGCCT
    CAAATTCAGC AGCAAGTGCC ACCAGTACCA CCACCCTCCA AACCGCAGTC AAACAATATT
    CCATCAGGTG CTAACATCCC ACCCCCTCCT CCTCCACCTC CACCTGCCTC AATGCCTCCA
    CCTGGTTCAG CCATGGCTGT TTTGAAGCTC GGCCCTCCAG CTGCAGCAGT AATTCCACAG
    ATCTCCCCTT CCCCCCCCTC ACCTCCTCCA CCACCACCTC CTCCTCCCAT AATGCCAGTG
    CAGTCACAGC CTCAACCACC TCCTCCACCC ATAATGCCCA TGCAGTCACA GCCTCTACCG
    CCTCCTCCAC CAATAACACC CATGCAGTCA CACAATCTAC CACCTCCTCC ACCCATGCAG
    TCACACGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCCATAACAC CCATGCAGTC ACATGCTCTA
    CCACCTCCTC CACCAATAAT GCCCATGCAG TCAGAGCCTC TACCGCCGCC TGCACAAATC
    ATGCCCATGC AATCACATCC TCTTCCACCA CCTCCACCTT TAGTGCAAAT GCAGTCACAG
    CCCATGCCAC CACCTCCTCC CACAGTGTCA TTCCAATCAC AGCCTTTACC ACCTCCTCCA
    CCCCCTCTCC AGGCTAATGG CCTTCCCCCT CCTCCACCTT TAATCACTAA TGGGCTTAAG
    CAGGCTTTTG CCCATAAATT TCCCAGCACA CCACAGGATG TATTACCCCC AGCTAATCAA
    AACAATTTTC CTCCACCTCC CCCACCACCA CCACCTCCAC CACTTGCACC TACGCTCCCT
    CCTTACCAGC ATGTTACTTC AACTTCAAAT CCACCACCAC CCCCACCACC ACCACCTCCT
    CCCCCACCAG CCCCCATACA TCTAGCAAAT CAACCTGCGG TTTTACCCAA ATTTTTCACT
    GGTGGATTTC CACCTCAGAA AGCACCCAAA CCTTCATGTG GCATTCTTCC AGTATCCCCA
    GTAGCTCCAG CACTTCAACC TCCACCACCG CCTCCTCCTC CACCTCCTCC TCCACCACCT
    GCACCATTGA AGAATCAGCC TCCACCTACA CTGCCCAAGC AGCACAGCCT CTCCAAGACA
    CTGCCCTTCT CCAGTCAAAC TTCTTCAGTA CCGTCTATGG TTAAGCAGCT TGCTAGTCAA
    TTTCCTGTTG CATCTCCTGC ATTAACCAAT CAAACAGATA GTCTCAAAGC TCCCCAGTCT
    CCACCTGCAG TGAAGGTTAA GCCAAAATGG CAGCCTGTAG GTCAAGGCCA GCAGCAACAG
    AGGTCACCTG AATTTCCACC ACCTCCACCA GACAATGCCC TTGGCTTTGC TTCACCAACA
    CCTCCACCAC CACCACCTCC TCCTCTTCCT CCAGGTCCTA CTCCTCCTCC TCCCCCTCCT
    CCTCCTCCTC TGATGTCAGG CTCCCCTATC AAAAAGTCTC CCTCTGGCTC ATCTGCAGGT
    GTTAAGAAAC CACCGCCAAC TCCTCAGCGC AACTCCAGTG TGAAGTACGA TGCCTCAACG
    GAATACCAGG AGTCCCGGAA GAACTTGGTG AGCAAGTTTA ACCCAAGTTC TGCTTCATCA
    ACAACATCAA CCTCCAGCTG TTCACCCTCA AAGGAGCTCT CTGCAGGCCC TCAAGCACCC
    CCCAAACCAG GGAAGCTCAA TCTGGCAAAT CTTCCTTTGG CTTTGCAGAA CAAATTCAAT
    CAGAACCGCC AGTCAGCGGC AGACTTTCCA TCTCCTCCAC CCCCAGAAAA TGATTTTTTC
    CCACCTCCGC CAATAGAGTC CGACCTCCCT CCACCACCAC CTCTTCCAGG GGATCTAAAT
    GGTGTGTCTC CCAAAGTGGC CGTAGTCAAT CCTCAGCCTC AGCCTGCTTG GGGCAAAAGT
    TCCCTAAAGA AGACACAACC TCCAGCATCA CTTCGCTGTA ATAACACTGT TAGAGAGTCT
    CCACCACTCT CACCACCTTC CATACACGGG GGGCCAATTC CCCATTCTCC CAAAGGCACC
    ACACAGCCAA ACTTTTTGGA GGACCTTAAC AAGACACTGA AACGCAAGTC TTCAAGAGTC
    TCTGGAGACA AAGTGGACCC TGTGGCCACA ATGGATGACA TGGTTTTGCC ACCACCTCCC
    CCTGAACTTC TGCAGGACCA GCAGAGGCTC AGTGGCAGTG GTTTCATGTC CGGGAACATC
    TCAGGCTATG CAACACTACG GCGAGGGCCT CCTCCTGCCC CACCCAAGCG GGACCACAGT
    ACCAAACTTA CAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05821
    Clone ID Related Accession (Same CDS sequence) XM_005159955.4 , XM_005159955.3 , NM_001365587.1
    Accession Version XM_005159955.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159955.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160012.1
    CDS309..4919
    Translation

    Target ORF information:

    RefSeq Version XM_005159955.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159955.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05821
    Clone ID Related Accession (Same CDS sequence) XM_005159955.4 , XM_005159955.3 , NM_001365587.1
    Accession Version XM_005159955.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160012.1
    CDS367..4977
    Translation

    Target ORF information:

    RefSeq Version XM_005159955.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159955.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCACTCGT ATCTGGACAG AGAGACCTCT CTGATTCTGA AAAACATTGC AGGAAAACCC
    TCTCACCTGC TGACCAAGGA AGAGCAGGCT GCCAAAGCGA AGGCTGAGAA AATACGTGTG
    GCTCTAGAGA AAATTAAAGA AGCCCAAGTG AAAAAGCTGG TGATACGTGT ACACATGTCA
    GATGAGAGCT CAAAGACCAT GATGGTGGAT GAGAGGCAAA CGGTCAGGCA GTTGCTGGAC
    AGTCTGCTGG ATAAATCTCA CTGCGGTTAC AGTCCTGATT GGGCTCTGGT GGAGACCATC
    CCTGAGCTAC AGATGGAGAG AATTTTTGAA GATCACGAGA ACCTTGTAGA GAACTTGCTC
    AACTGGACCA GAGACAGTCA GAACAAACTC ATGTTCATTG AACGGATTGA AAAGTATGCA
    CTTTTCAAAA ACCCTCAGAA CTATTTACTT GGGAGGAAGG AGACTTCTGA GATGGCAGAC
    AGAAACAAGG AGGCACTGCT AGAGGAATGT TTTTGTGGCA GCTCGGTGTC AGTTCCTGAA
    ATCGAAGGAG TGTTGTGGTT GAAGGAAGAT GGTAAAAAGT CTTGGAAGAA GCGCTACTTT
    CTGCTAAGGG CATCTGGCAT CTATTTTGTG CCAAAAGGCA AAGCCAAGGC TTCCAGGGAT
    TTGGTGTGCT TCCTGCAGTT GGACCATGTT AACGTGTATT ATGGTCAGGA CTACAGAAGC
    AAGTACAAGG CCCCCACTGA CTACTGCCTG GCCCTCAAGC ACCCTCAGAT CCAGAAAAAG
    TCACAGTACA TCAAATATCT ATGCTGTGAT GATGTCAGAA CTTTACACCA GTGGGTAAAT
    GGCATCCGTA TTGCTAAGTA CGGGAAGCAG TTGTATGTGA ACTACCAGGA AGCTATGAAG
    CGAACAGAGG CTGCCTATGA CTGGTCTTCT CTCTCCAGCA CCAGCATCAA GTCAGGAACA
    AGCTCAGTCA GTATACCTGA GTCGCAGTCA AACCACTCCA GCCAGACAGA CAGTGGTATG
    TCAGATGGTA CTTCGTCCTC TCATGCCCGC TCTCAAAGTG TGGTCAGTTC AATCTTTTCT
    GAGGCCTGGA AGAGAGGAAC TCAAATAGAG GAGAGCACTA AGGCAAGGCC AGAATCAATC
    CGTTCTACTC ATTCAAGCCA TAGCAGTCAT TCTTCTCATC ACGTATCTCA ACAGCAACAT
    CTACACGCAC AACAGCCACA TGTGCATTCA ATTCTACAAT CACGTGGAAG TGTCACCCAG
    GCTCCGGCAC AGGCACCAAC ACAAACACTG GCACAAGCAC CACCCCAAGT GGCTCCAGCT
    CCACCACCTC CTCCTCCACC TCCTCCTCCA CCACCTCCTC CTCCTATTTC CAGCATCCCT
    CACCATGCAA CTCCTACCAT GTTTGCCAAA TACAGCACTA TCACTCGGCT GCAAAATGCC
    TCTCAGGCTA CAAGTCACCA CAAGCCTCAG CCTCAAATTC AGCAGCAAGT GCCACCAGTA
    CCACCACCCT CCAAACCGCA GTCAAACAAT ATTCCATCAG GTGCTAACAT CCCACCCCCT
    CCTCCTCCAC CTCCACCTGC CTCAATGCCT CCACCTGGTT CAGCCATGGC TGTTTTGAAG
    CTCGGCCCTC CAGCTGCAGC AGTAATTCCA CAGATCTCCC CTTCCCCCCC CTCACCTCCT
    CCACCACCAC CTCCTCCTCC CATAATGCCA GTGCAGTCAC AGCCTCAACC ACCTCCTCCA
    CCCATAATGC CCATGCAGTC ACAGCCTCTA CCGCCTCCTC CACCAATAAC ACCCATGCAG
    TCACACAATC TACCACCTCC TCCACCCATG CAGTCACACG CTCTACCACC TCCTCCACCC
    ATAACACCCA TGCAGTCACA TGCTCTACCA CCTCCTCCAC CCATAACACC CATGCAGTCA
    CATGCTCTAC CACCTCCTCC ACCCATAACA CCCATGCAGT CACATGCTCT ACCACCTCCT
    CCACCCATAA CACCCATGCA GTCACATGCT CTACCACCTC CTCCACCAAT AATGCCCATG
    CAGTCAGAGC CTCTACCGCC GCCTGCACAA ATCATGCCCA TGCAATCACA TCCTCTTCCA
    CCACCTCCAC CTTTAGTGCA AATGCAGTCA CAGCCCATGC CACCACCTCC TCCCACAGTG
    TCATTCCAAT CACAGCCTTT ACCACCTCCT CCACCCCCTC TCCAGGCTAA TGGCCTTCCC
    CCTCCTCCAC CTTTAATCAC TAATGGGCTT AAGCAGGCTT TTGCCCATAA ATTTCCCAGC
    ACACCACAGG ATGTATTACC CCCAGCTAAT CAAAACAATT TTCCTCCACC TCCCCCACCA
    CCACCACCTC CACCACTTGC ACCTACGCTC CCTCCTTACC AGCATGTTAC TTCAACTTCA
    AATCCACCAC CACCCCCACC ACCACCACCT CCTCCCCCAC CAGCCCCCAT ACATCTAGCA
    AATCAACCTG CGGTTTTACC CAAATTTTTC ACTGGTGGAT TTCCACCTCA GAAAGCACCC
    AAACCTTCAT GTGGCATTCT TCCAGTATCC CCAGTAGCTC CAGCACTTCA ACCTCCACCA
    CCGCCTCCTC CTCCACCTCC TCCTCCACCA CCTGCACCAT TGAAGAATCA GCCTCCACCT
    ACACTGCCCA AGCAGCACAG CCTCTCCAAG ACACTGCCCT TCTCCAGTCA AACTTCTTCA
    GTACCGTCTA TGGTTAAGCA GCTTGCTAGT CAATTTCCTG TTGCATCTCC TGCATTAACC
    AATCAAACAG ATAGTCTCAA AGCTCCCCAG TCTCCACCTG CAGTGAAGGT TAAGCCAAAA
    TGGCAGCCTG TAGGTCAAGG CCAGCAGCAA CAGAGGTCAC CTGAATTTCC ACCACCTCCA
    CCAGACAATG CCCTTGGCTT TGCTTCACCA ACACCTCCAC CACCACCACC TCCTCCTCTT
    CCTCCAGGTC CTACTCCTCC TCCTCCCCCT CCTCCTCCTC CTCTGATGTC AGGCTCCCCT
    ATCAAAAAGT CTCCCTCTGG CTCATCTGCA GGTGTTAAGA AACCACCGCC AACTCCTCAG
    CGCAACTCCA GTGTGAAGTA CGATGCCTCA ACGGAATACC AGGAGTCCCG GAAGAACTTG
    GTGAGCAAGT TTAACCCAAG TTCTGCTTCA TCAACAACAT CAACCTCCAG CTGTTCACCC
    TCAAAGGAGC TCTCTGCAGG CCCTCAAGCA CCCCCCAAAC CAGGGAAGCT CAATCTGGCA
    AATCTTCCTT TGGCTTTGCA GAACAAATTC AATCAGAACC GCCAGTCAGC GGCAGACTTT
    CCATCTCCTC CACCCCCAGA AAATGATTTT TTCCCACCTC CGCCAATAGA GTCCGACCTC
    CCTCCACCAC CACCTCTTCC AGGGGATCTA AATGGTGTGT CTCCCAAAGT GGCCGTAGTC
    AATCCTCAGC CTCAGCCTGC TTGGGGCAAA AGTTCCCTAA AGAAGACACA ACCTCCAGCA
    TCACTTCGCT GTAATAACAC TGTTAGAGAG TCTCCACCAC TCTCACCACC TTCCATACAC
    GGGGGGCCAA TTCCCCATTC TCCCAAAGGC ACCACACAGC CAAACTTTTT GGAGGACCTT
    AACAAGACAC TGAAACGCAA GTCTTCAAGA GTCTCTGGAG ACAAAGTGGA CCCTGTGGCC
    ACAATGGATG ACATGGTTTT GCCACCACCT CCCCCTGAAC TTCTGCAGGA CCAGCAGAGG
    CTCAGTGGCA GTGGTTTCAT GTCCGGGAAC ATCTCAGGCT ATGCAACACT ACGGCGAGGG
    CCTCCTCCTG CCCCACCCAA GCGGGACCAC AGTACCAAAC TTACAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05822
    Clone ID Related Accession (Same CDS sequence) XM_005159956.3 , NM_001365589.1 , XM_005159956.4
    Accession Version XM_005159956.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160013.1
    CDS366..4901
    Translation

    Target ORF information:

    RefSeq Version XM_005159956.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05822
    Clone ID Related Accession (Same CDS sequence) XM_005159956.3 , NM_001365589.1 , XM_005159956.4
    Accession Version XM_005159956.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159956.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160013.1
    CDS252..4787
    Translation

    Target ORF information:

    RefSeq Version XM_005159956.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159956.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGGAAGAGC AGGCTGCCAA AGCGAAGGCT GAGAAAATAC GTGTGGCTCT AGAGAAAATT
    AAAGAAGCCC AAGTGAAAAA GCTGGTGATA CGTGTACACA TGTCAGATGA GAGCTCAAAG
    ACCATGATGG TGGATGAGAG GCAAACGGTC AGGCAGTTGC TGGACAGTCT GCTGGATAAA
    TCTCACTGCG GTTACAGTCC TGATTGGGCT CTGGTGGAGA CCATCCCTGA GCTACAGATG
    GAGAGAATTT TTGAAGATCA CGAGAACCTT GTAGAGAACT TGCTCAACTG GACCAGAGAC
    AGTCAGAACA AACTCATGTT CATTGAACGG ATTGAAAAGT ATGCACTTTT CAAAAACCCT
    CAGAACTATT TACTTGGGAG GAAGGAGACT TCTGAGATGG CAGACAGAAA CAAGGAGGCA
    CTGCTAGAGG AATGTTTTTG TGGCAGCTCG GTGTCAGTTC CTGAAATCGA AGGAGTGTTG
    TGGTTGAAGG AAGATGGTAA AAAGTCTTGG AAGAAGCGCT ACTTTCTGCT AAGGGCATCT
    GGCATCTATT TTGTGCCAAA AGGCAAAGCC AAGGCTTCCA GGGATTTGGT GTGCTTCCTG
    CAGTTGGACC ATGTTAACGT GTATTATGGT CAGGACTACA GAAGCAAGTA CAAGGCCCCC
    ACTGACTACT GCCTGGCCCT CAAGCACCCT CAGATCCAGA AAAAGTCACA GTACATCAAA
    TATCTATGCT GTGATGATGT CAGAACTTTA CACCAGTGGG TAAATGGCAT CCGTATTGCT
    AAGTACGGGA AGCAGTTGTA TGTGAACTAC CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC
    TATGACTGGT CTTCTCTCTC CAGCACCAGC ATCAAGTCAG GAACAAGCTC AGTCAGTATA
    CCTGAGTCGC AGTCAAACCA CTCCAGCCAG ACAGACAGTG GTATGTCAGA TGGTACTTCG
    TCCTCTCATG CCCGCTCTCA AAGTGTGGTC AGTTCAATCT TTTCTGAGGC CTGGAAGAGA
    GGAACTCAAA TAGAGGAGAG CACTAAGGCA AGGCCAGAAT CAATCCGTTC TACTCATTCA
    AGCCATAGCA GTCATTCTTC TCATCACGTA TCTCAACAGC AACATCTACA CGCACAACAG
    CCACATGTGC ATTCAATTCT ACAATCACGT GGAAGTGTCA CCCAGGCTCC GGCACAGGCA
    CCAACACAAA CACTGGCACA AGCACCACCC CAAGTGGCTC CAGCTCCACC ACCTCCTCCT
    CCACCTCCTC CTCCACCACC TCCTCCTCCT ATTTCCAGCA TCCCTCACCA TGCAACTCCT
    ACCATGTTTG CCAAATACAG CACTATCACT CGGCTGCAAA ATGCCTCTCA GGCTACAAGT
    CACCACAAGC CTCAGCCTCA AATTCAGCAG CAAGTGCCAC CAGTACCACC ACCCTCCAAA
    CCGCAGTCAA ACAATATTCC ATCAGGTGCT AACATCCCAC CCCCTCCTCC TCCACCTCCA
    CCTGCCTCAA TGCCTCCACC TGGTTCAGCC ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT
    GCAGCAGTAA TTCCACAGAT CTCCCCTTCC CCCCCCTCAC CTCCTCCACC ACCACCTCCT
    CCTCCCATAA TGCCAGTGCA GTCACAGCCT CAACCACCTC CTCCACCCAT AATGCCCATG
    CAGTCACAGC CTCTACCGCC TCCTCCACCA ATAACACCCA TGCAGTCACA CAATCTACCA
    CCTCCTCCAC CCATGCAGTC ACACGCTCTA CCACCTCCTC CACCCATAAC ACCCATGCAG
    TCACATGCTC TACCACCTCC TCCACCCATA ACACCCATGC AGTCACATGC TCTACCACCT
    CCTCCACCCA TAACACCCAT GCAGTCACAT GCTCTACCAC CTCCTCCACC CATAACACCC
    ATGCAGTCAC ATGCTCTACC ACCTCCTCCA CCAATAATGC CCATGCAGTC AGAGCCTCTA
    CCGCCGCCTG CACAAATCAT GCCCATGCAA TCACATCCTC TTCCACCACC TCCACCTTTA
    GTGCAAATGC AGTCACAGCC CATGCCACCA CCTCCTCCCA CAGTGTCATT CCAATCACAG
    CCTTTACCAC CTCCTCCACC CCCTCTCCAG GCTAATGGCC TTCCCCCTCC TCCACCTTTA
    ATCACTAATG GGCTTAAGCA GGCTTTTGCC CATAAATTTC CCAGCACACC ACAGGATGTA
    TTACCCCCAG CTAATCAAAA CAATTTTCCT CCACCTCCCC CACCACCACC ACCTCCACCA
    CTTGCACCTA CGCTCCCTCC TTACCAGCAT GTTACTTCAA CTTCAAATCC ACCACCACCC
    CCACCACCAC CACCTCCTCC CCCACCAGCC CCCATACATC TAGCAAATCA ACCTGCGGTT
    TTACCCAAAT TTTTCACTGG TGGATTTCCA CCTCAGAAAG CACCCAAACC TTCATGTGGC
    ATTCTTCCAG TATCCCCAGT AGCTCCAGCA CTTCAACCTC CACCACCGCC TCCTCCTCCA
    CCTCCTCCTC CACCACCTGC ACCATTGAAG AATCAGCCTC CACCTACACT GCCCAAGCAG
    CACAGCCTCT CCAAGACACT GCCCTTCTCC AGTCAAACTT CTTCAGTACC GTCTATGGTT
    AAGCAGCTTG CTAGTCAATT TCCTGTTGCA TCTCCTGCAT TAACCAATCA AACAGATAGT
    CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG AAGGTTAAGC CAAAATGGCA GCCTGTAGGT
    CAAGGCCAGC AGCAACAGAG GTCACCTGAA TTTCCACCAC CTCCACCAGA CAATGCCCTT
    GGCTTTGCTT CACCAACACC TCCACCACCA CCACCTCCTC CTCTTCCTCC AGGTCCTACT
    CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG ATGTCAGGCT CCCCTATCAA AAAGTCTCCC
    TCTGGCTCAT CTGCAGGTGT TAAGAAACCA CCGCCAACTC CTCAGCGCAA CTCCAGTGTG
    AAGTACGATG CCTCAACGGA ATACCAGGAG TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC
    CCAAGTTCTG CTTCATCAAC AACATCAACC TCCAGCTGTT CACCCTCAAA GGAGCTCTCT
    GCAGGCCCTC AAGCACCCCC CAAACCAGGG AAGCTCAATC TGGCAAATCT TCCTTTGGCT
    TTGCAGAACA AATTCAATCA GAACCGCCAG TCAGCGGCAG ACTTTCCATC TCCTCCACCC
    CCAGAAAATG ATTTTTTCCC ACCTCCGCCA ATAGAGTCCG ACCTCCCTCC ACCACCACCT
    CTTCCAGGGG ATCTAAATGG TGTGTCTCCC AAAGTGGCCG TAGTCAATCC TCAGCCTCAG
    CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG ACACAACCTC CAGCATCACT TCGCTGTAAT
    AACACTGTTA GAGAGTCTCC ACCACTCTCA CCACCTTCCA TACACGGGGG GCCAATTCCC
    CATTCTCCCA AAGGCACCAC ACAGCCAAAC TTTTTGGAGG ACCTTAACAA GACACTGAAA
    CGCAAGTCTT CAAGAGTCTC TGGAGACAAA GTGGACCCTG TGGCCACAAT GGATGACATG
    GTTTTGCCAC CACCTCCCCC TGAACTTCTG CAGGACCAGC AGAGGCTCAG TGGCAGTGGT
    TTCATGTCCG GGAACATCTC AGGCTATGCA ACACTACGGC GAGGGCCTCC TCCTGCCCCA
    CCCAAGCGGG ACCACAGTAC CAAACTTACA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05823
    Clone ID Related Accession (Same CDS sequence) XM_005159957.3 , XM_005159957.4
    Accession Version XM_005159957.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
    GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA
    CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC
    AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT
    CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT
    GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG
    ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT
    TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG
    GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG
    AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC
    AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT
    CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT
    CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA
    CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC
    CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC
    ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG
    ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC
    AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA
    AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA
    TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT
    GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC
    CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT
    ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT
    CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG
    CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT
    AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC
    ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC
    CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT
    CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA
    ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA
    CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA
    ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT
    GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA
    CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA
    TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA
    CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG
    GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC
    CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT
    CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT
    GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC
    CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA
    CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA
    CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG
    AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC
    AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA
    TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG
    AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA
    TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA
    CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG
    ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA
    CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG
    TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC
    TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG
    AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG
    TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA
    ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC
    AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG
    ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA
    CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC
    TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA
    GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG
    CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA
    ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160014.1
    CDS1258..5043
    Translation

    Target ORF information:

    RefSeq Version XM_005159957.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159957.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
    GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA
    CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC
    AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT
    CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT
    GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG
    ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT
    TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG
    GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG
    AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC
    AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT
    CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT
    CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA
    CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC
    CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC
    ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG
    ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC
    AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA
    AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA
    TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT
    GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC
    CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT
    ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT
    CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG
    CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT
    AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC
    ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC
    CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT
    CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA
    ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA
    CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA
    ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT
    GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA
    CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA
    TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA
    CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG
    GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC
    CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT
    CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT
    GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC
    CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA
    CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA
    CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG
    AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC
    AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA
    TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG
    AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA
    TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA
    CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG
    ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA
    CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG
    TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC
    TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG
    AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG
    TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA
    ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC
    AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG
    ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA
    CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC
    TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA
    GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG
    CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA
    ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05823
    Clone ID Related Accession (Same CDS sequence) XM_005159957.3 , XM_005159957.4
    Accession Version XM_005159957.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159957.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
    GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA
    CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC
    AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT
    CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT
    GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG
    ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT
    TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG
    GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG
    AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC
    AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT
    CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT
    CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA
    CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC
    CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC
    ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG
    ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC
    AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA
    AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA
    TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT
    GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC
    CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT
    ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT
    CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG
    CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT
    AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC
    ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC
    CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT
    CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA
    ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA
    CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA
    ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT
    GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA
    CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA
    TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA
    CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG
    GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC
    CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT
    CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT
    GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC
    CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA
    CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA
    CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG
    AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC
    AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA
    TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG
    AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA
    TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA
    CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG
    ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA
    CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG
    TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC
    TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG
    AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG
    TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA
    ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC
    AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG
    ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA
    CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC
    TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA
    GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG
    CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA
    ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160014.1
    CDS927..4712
    Translation

    Target ORF information:

    RefSeq Version XM_005159957.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159957.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGA TGACTGCATG CTGGAGGGGG CAGGAAGAGC AGGCTGCCAA AGCGAAGGCT 
    GAGAAAATAC GTGTGGCTCT AGAGAAAATT AAAGAAGCCC AAGTGAAAAA GCTGGTGATA
    CGTGTACACA TGTCAGATGA GAGCTCAAAG ACCATGATGG TGGATGAGAG GCAAACGGTC
    AGGCAGTTGC TGGACAGTCT GCTGGATAAA TCTCACTGCG GTTACAGTCC TGATTGGGCT
    CTGGTGGAGA CCATCCCTGA GCTACAGATG GAGAGAATTT TTGAAGATCA CGAGAACCTT
    GTAGAGAACT TGCTCAACTG GACCAGAGAC AGTCAGAACA AACTCATGTT CATTGAACGG
    ATTGAAAAGT ATGCACTTTT CAAAAACCCT CAGAACTATT TACTTGGGAG GAAGGAGACT
    TCTGAGATGG CAGACAGAAA CAAGGAGGCA CTGCTAGAGG AATGTTTTTG TGGCAGCTCG
    GTGTCAGTTC CTGAAATCGA AGGAGTGTTG TGGTTGAAGG AAGATGGTAA AAAGTCTTGG
    AAGAAGCGCT ACTTTCTGCT AAGGGCATCT GGCATCTATT TTGTGCCAAA AGGCAAAGCC
    AAGGCTTCCA GGGATTTGGT GTGCTTCCTG CAGTTGGACC ATGTTAACGT GTATTATGGT
    CAGGACTACA GAAGCAAGTA CAAGGCCCCC ACTGACTACT GCCTGGCCCT CAAGCACCCT
    CAGATCCAGA AAAAGTCACA GTACATCAAA TATCTATGCT GTGATGATGT CAGAACTTTA
    CACCAGTGGG TAAATGGCAT CCGTATTGCT AAGTACGGGA AGCAGTTGTA TGTGAACTAC
    CAGGAAGCTA TGAAGCGAAC AGAGGCTGCC TATGACTGGT CTTCTCTCTC CAGCACCAGC
    ATCAAGTCAG GAACAAGCTC AGTCAGTATA CCTGAGTCGC AGTCAAACCA CTCCAGCCAG
    ACAGACAGTG GTATGTCAGA TGGTACTTCG TCCTCTCATG CCCGCTCTCA AAGTGTGGTC
    AGTTCAATCT TTTCTGAGGC CTGGAAGAGA GGAACTCAAA TAGAGGAGAG CACTAAGGCA
    AGGCCAGAAT CAATCCGTTC TACTCATTCA AGCCATAGCA GTCATTCTTC TCATCACGTA
    TCTCAACAGC AACATCTACA CGCACAACAG CCACATGTGC ATTCAATTCT ACAATCACGT
    GGAAGTGTCA CCCAGGCTCC GGCACAGGCA CCAACACAAA CACTGGCACA AGCACCACCC
    CAAGTGGCTC CAGCTCCACC ACCTCCTCCT CCACCTCCTC CTCCACCACC TCCTCCTCCT
    ATTTCCAGCA TCCCTCACCA TGCAACTCCT ACCATGTTTG CCAAATACAG CACTATCACT
    CGGCTGCAAA ATGCCTCTCA GGCTACAAGT CACCACAAGC CTCAGCCTCA AATTCAGCAG
    CAAGTGCCAC CAGTACCACC ACCCTCCAAA CCGCAGTCAA ACAATATTCC ATCAGGTGCT
    AACATCCCAC CCCCTCCTCC TCCACCTCCA CCTGCCTCAA TGCCTCCACC TGGTTCAGCC
    ATGGCTGTTT TGAAGCTCGG CCCTCCAGCT GCAGCAGTAA TTCCACAGAT CTCCCCTTCC
    CCCCCCTCAC CTCCTCCACC ACCACCTCCT CCTCCCATAA TGCCAGTGCA GTCACAGCCT
    CAACCACCTC CTCCACCCAT AATGCCCATG CAGTCACAGC CTCTACCGCC TCCTCCACCA
    ATAACACCCA TGCAGTCACA CAATCTACCA CCTCCTCCAC CCATGCAGTC ACACGCTCTA
    CCACCTCCTC CACCCATAAC ACCCATGCAG TCACATGCTC TACCACCTCC TCCACCCATA
    ACACCCATGC AGTCACATGC TCTACCACCT CCTCCACCCA TAACACCCAT GCAGTCACAT
    GCTCTACCAC CTCCTCCACC CATAACACCC ATGCAGTCAC ATGCTCTACC ACCTCCTCCA
    CCAATAATGC CCATGCAGTC AGAGCCTCTA CCGCCGCCTG CACAAATCAT GCCCATGCAA
    TCACATCCTC TTCCACCACC TCCACCTTTA GTGCAAATGC AGTCACAGCC CATGCCACCA
    CCTCCTCCCA CAGTGTCATT CCAATCACAG CCTTTACCAC CTCCTCCACC CCCTCTCCAG
    GCTAATGGCC TTCCCCCTCC TCCACCTTTA ATCACTAATG GGCTTAAGCA GGCTTTTGCC
    CATAAATTTC CCAGCACACC ACAGGATGTA TTACCCCCAG CTAATCAAAA CAATTTTCCT
    CCACCTCCCC CACCACCACC ACCTCCACCA CTTGCACCTA CGCTCCCTCC TTACCAGCAT
    GTTACTTCAA CTTCAAATCC ACCACCACCC CCACCACCAC CACCTCCTCC CCCACCAGCC
    CCCATACATC TAGCAAATCA ACCTGCGGTT TTACCCAAAT TTTTCACTGG TGGATTTCCA
    CCTCAGAAAG CACCCAAACC TTCATGTGGC ATTCTTCCAG TATCCCCAGT AGCTCCAGCA
    CTTCAACCTC CACCACCGCC TCCTCCTCCA CCTCCTCCTC CACCACCTGC ACCATTGAAG
    AATCAGCCTC CACCTACACT GCCCAAGCAG CACAGCCTCT CCAAGACACT GCCCTTCTCC
    AGTCAAACTT CTTCAGTACC GTCTATGGTT AAGCAGCTTG CTAGTCAATT TCCTGTTGCA
    TCTCCTGCAT TAACCAATCA AACAGATAGT CTCAAAGCTC CCCAGTCTCC ACCTGCAGTG
    AAGGTTAAGC CAAAATGGCA GCCTGTAGGT CAAGGCCAGC AGCAACAGAG GTCACCTGAA
    TTTCCACCAC CTCCACCAGA CAATGCCCTT GGCTTTGCTT CACCAACACC TCCACCACCA
    CCACCTCCTC CTCTTCCTCC AGGTCCTACT CCTCCTCCTC CCCCTCCTCC TCCTCCTCTG
    ATGTCAGGCT CCCCTATCAA AAAGTCTCCC TCTGGCTCAT CTGCAGGTGT TAAGAAACCA
    CCGCCAACTC CTCAGCGCAA CTCCAGTGTG AAGTACGATG CCTCAACGGA ATACCAGGAG
    TCCCGGAAGA ACTTGGTGAG CAAGTTTAAC CCAAGTTCTG CTTCATCAAC AACATCAACC
    TCCAGCTGTT CACCCTCAAA GGAGCTCTCT GCAGGCCCTC AAGCACCCCC CAAACCAGGG
    AAGCTCAATC TGGCAAATCT TCCTTTGGCT TTGCAGAACA AATTCAATCA GAACCGCCAG
    TCAGCGGCAG ACTTTCCATC TCCTCCACCC CCAGAAAATG ATTTTTTCCC ACCTCCGCCA
    ATAGAGTCCG ACCTCCCTCC ACCACCACCT CTTCCAGGGG ATCTAAATGG TGTGTCTCCC
    AAAGTGGCCG TAGTCAATCC TCAGCCTCAG CCTGCTTGGG GCAAAAGTTC CCTAAAGAAG
    ACACAACCTC CAGCATCACT TCGCTGTAAT AACACTGTTA GAGAGTCTCC ACCACTCTCA
    CCACCTTCCA TACACGGGGG GCCAATTCCC CATTCTCCCA AAGGCACCAC ACAGCCAAAC
    TTTTTGGAGG ACCTTAACAA GACACTGAAA CGCAAGTCTT CAAGAGTCTC TGGAGACAAA
    GTGGACCCTG TGGCCACAAT GGATGACATG GTTTTGCCAC CACCTCCCCC TGAACTTCTG
    CAGGACCAGC AGAGGCTCAG TGGCAGTGGT TTCATGTCCG GGAACATCTC AGGCTATGCA
    ACACTACGGC GAGGGCCTCC TCCTGCCCCA CCCAAGCGGG ACCACAGTAC CAAACTTACA
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43264
    Clone ID Related Accession (Same CDS sequence) XM_009291873.3 , XM_009291873.2
    Accession Version XM_009291873.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG
    AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT
    GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG
    CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG
    GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA
    GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT
    GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT
    GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG
    TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG
    AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG
    GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG
    GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG
    ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC
    CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG
    GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC
    AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA
    GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT
    TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG
    CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT
    CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA
    AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA
    GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT
    TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG
    CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA
    GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC
    ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG
    GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC
    CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA
    CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA
    ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA
    ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA
    CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT
    CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT
    AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT
    AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA
    CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT
    ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC
    ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT
    CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT
    CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT
    CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT
    CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT
    CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG
    GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT
    CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA
    CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG
    TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG
    CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC
    CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC
    AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG
    CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA
    GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA
    GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA
    GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA
    CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA
    CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT
    TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG
    GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG
    GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA
    CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290148.1
    CDS309..4931
    Translation

    Target ORF information:

    RefSeq Version XM_009291873.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291873.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG
    AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT
    GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG
    CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG
    GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA
    GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT
    GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT
    GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG
    TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG
    AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG
    GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG
    GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG
    ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC
    CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG
    GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC
    AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA
    GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT
    TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG
    CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT
    CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA
    AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA
    GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT
    TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG
    CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA
    GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC
    ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG
    GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC
    CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA
    CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA
    ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA
    ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA
    CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT
    CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT
    AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT
    AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA
    CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT
    ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC
    ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT
    CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT
    CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT
    CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT
    CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT
    CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG
    GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT
    CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA
    CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG
    TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG
    CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC
    CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC
    AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG
    CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA
    GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA
    GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA
    GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA
    CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA
    CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT
    TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG
    GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG
    GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA
    CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43264
    Clone ID Related Accession (Same CDS sequence) XM_009291873.3 , XM_009291873.2
    Accession Version XM_009291873.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-associated and pleckstrin homology domains-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG
    AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT
    GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG
    CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG
    GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA
    GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT
    GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT
    GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG
    TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG
    AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG
    GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG
    GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG
    ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC
    CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG
    GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC
    AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA
    GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT
    TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG
    CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT
    CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA
    AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA
    GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT
    TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG
    CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA
    GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC
    ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG
    GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC
    CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA
    CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA
    ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA
    ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA
    CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT
    CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT
    AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT
    AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA
    CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT
    ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC
    ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT
    CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT
    CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT
    CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT
    CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT
    CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG
    GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT
    CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA
    CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG
    TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG
    CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC
    CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC
    AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG
    CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA
    GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA
    GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA
    GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA
    CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA
    CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT
    TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG
    GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG
    GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA
    CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290148.1
    CDS367..4989
    Translation

    Target ORF information:

    RefSeq Version XM_009291873.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras association (RalGDS/AF-6) and pleckstrin homology domains 1b (raph1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGCAGG TATCAGATGA TGAGCTGGAC CATGCTGCCG AAGAAGACAG CGATAAGGAG 
    GACCAGGATC TGGACAAGAT GTTTGGTGCC TGGTTGGGAG AACTGGACAA GCTCACACAG
    AGTTTGGATG ATGGGCGTCC TGAGAAAGTA CAAAAAGCTC CTCTCAGACA GGAAACAAAC
    CTTGCCAATT TCTCCTATCG TTTCTCTATG TACAACATCA ATGAAGCCAT TAATCAGGGT
    GAGACGGTGG ACCTGGATGC TCTCATGGCT GACCTGTGCT CAATAGAGCA AGAACTCAGC
    ACCATAGGCA AACCCATAAG CAAGACCTTG GATGGCAAAT CCAAACAAAG GCCTACAGGA
    CGCTCAGCCA GCGCAAAACA CAGCGGAGGG GGCAGCAGTG GAGGAAGCAC CAGCAGCAGT
    ACCCGAGCCT CACCCGCATC CACTGTTCGG GGTGGCAACA GTCAATCTCG ACCCCTGGCT
    TCCAACTTCT CCCTGGATGA CATCACTGCT CAGCTGGAAA AGGCGTCTTT GAGCATGGAT
    GAAGCAGCGA AACAGACTTC TGAGTCTTCC TCCTCATCTT ATTCATCTGC CAATCCACCT
    TCTAACTCAC ATCATCGGCG AACAGGGTCA GTCGGGACAG TCAGTGACCA GGAAGTGCGT
    TCTATCGGTC ATTCATCCAG GTCAAGCGTC AACTCTGCCT CTGCATCCAG CATGGACTCC
    CTGGATATTC GAGGACAGGA GAACGAGACT CAGTCACAAA ACCAAAGCCA GACTAGCACA
    GAGCATGTGT TACTGCGCAG AGCCAATGGT AAAACTACCA GACGGCATCT TGACTTTACC
    TCACAGGAGG GGGAGGTGGA TCAAAGTACT GAAGAGCAGG CTGCCAAAGC GAAGGCTGAG
    AAAATACGTG TGGCTCTAGA GAAAATTAAA GAAGCCCAAG TGAAAAAGCT GGTGATACGT
    GTACACATGT CAGATGAGAG CTCAAAGACC ATGATGGTGG ATGAGAGGCA AACGGTCAGG
    CAGTTGCTGG ACAGTCTGCT GGATAAATCT CACTGCGGTT ACAGTCCTGA TTGGGCTCTG
    GTGGAGACCA TCCCTGAGCT ACAGATGGAG AGAATTTTTG AAGATCACGA GAACCTTGTA
    GAGAACTTGC TCAACTGGAC CAGAGACAGT CAGAACAAAC TCATGTTCAT TGAACGGATT
    GAAAAGTATG CACTTTTCAA AAACCCTCAG AACTATTTAC TTGGGAGGAA GGAGACTTCT
    GAGATGGCAG ACAGAAACAA GGAGGCACTG CTAGAGGAAT GTTTTTGTGG CAGCTCGGTG
    TCAGTTCCTG AAATCGAAGG AGTGTTGTGG TTGAAGGAAG ATGGTAAAAA GTCTTGGAAG
    AAGCGCTACT TTCTGCTAAG GGCATCTGGC ATCTATTTTG TGCCAAAAGG CAAAGCCAAG
    GCTTCCAGGG ATTTGGTGTG CTTCCTGCAG TTGGACCATG TTAACGTGTA TTATGGTCAG
    GACTACAGAA GCAAGTACAA GGCCCCCACT GACTACTGCC TGGCCCTCAA GCACCCTCAG
    ATCCAGAAAA AGTCACAGTA CATCAAATAT CTATGCTGTG ATGATGTCAG AACTTTACAC
    CAGTGGGTAA ATGGCATCCG TATTGCTAAG TACGGGAAGC AGTTGTATGT GAACTACCAG
    GAAGCTATGA AGCGAACAGA GGCTGCCTAT GACTGGTCTT CTCTCTCCAG CACCAGCATC
    AAGTCAGGAA CAAGCTCAGT CAGTATACCT GAGTCGCAGT CAAACCACTC CAGCCAGACA
    GACAGTGGTA TGTCAGATGG TACTTCGTCC TCTCATGCCC GCTCTCAAAG TGTGGTCAGT
    TCAATCTTTT CTGAGGCCTG GAAGAGAGGA ACTCAAATAG AGGAGAGCAC TAAGGCAAGG
    CCAGAATCAA TCCGTTCTAC TCATTCAAGC CATAGCAGTC ATTCTTCTCA TCACGTATCT
    CAACAGCAAC ATCTACACGC ACAACAGCCA CATGTGCATT CAATTCTACA ATCACGTGGA
    AGTGTCACCC AGGCTCCGGC ACAGGCACCA ACACAAACAC TGGCACAAGC ACCACCCCAA
    GTGGCTCCAG CTCCACCACC TCCTCCTCCA CCTCCTCCTC CACCACCTCC TCCTCCTATT
    TCCAGCATCC CTCACCATGC AACTCCTACC ATGTTTGCCA AATACAGCAC TATCACTCGG
    CTGCAAAATG CCTCTCAGGC TACAAGTCAC CACAAGCCTC AGCCTCAAAT TCAGCAGCAA
    GTGCCACCAG TACCACCACC CTCCAAACCG CAGTCAAACA ATATTCCATC AGGTGCTAAC
    ATCCCACCCC CTCCTCCTCC ACCTCCACCT GCCTCAATGC CTCCACCTGG TTCAGCCATG
    GCTGTTTTGA AGCTCGGCCC TCCAGCTGCA GCAGTAATTC CACAGATCTC CCCTTCCCCC
    CCCTCACCTC CTCCACCACC ACCTCCTCCT CCCATAATGC CAGTGCAGTC ACAGCCTCAA
    CCACCTCCTC CACCCATAAT GCCCATGCAG TCACAGCCTC TACCGCCTCC TCCACCAATA
    ACACCCATGC AGTCACACAA TCTACCACCT CCTCCACCCA TGCAGTCACA CGCTCTACCA
    CCTCCTCCAC CCATAACACC CATGCAGTCA CATGCTCTAC CACCTCCTCC ACCCATAACA
    CCCATGCAGT CACATGCTCT ACCACCTCCT CCACCCATAA CACCCATGCA GTCACATGCT
    CTACCACCTC CTCCACCCAT AACACCCATG CAGTCACATG CTCTACCACC TCCTCCACCA
    ATAATGCCCA TGCAGTCAGA GCCTCTACCG CCGCCTGCAC AAATCATGCC CATGCAATCA
    CATCCTCTTC CACCACCTCC ACCTTTAGTG CAAATGCAGT CACAGCCCAT GCCACCACCT
    CCTCCCACAG TGTCATTCCA ATCACAGCCT TTACCACCTC CTCCACCCCC TCTCCAGGCT
    AATGGCCTTC CCCCTCCTCC ACCTTTAATC ACTAATGGGC TTAAGCAGGC TTTTGCCCAT
    AAATTTCCCA GCACACCACA GGATGTATTA CCCCCAGCTA ATCAAAACAA TTTTCCTCCA
    CCTCCCCCAC CACCACCACC TCCACCACTT GCACCTACGC TCCCTCCTTA CCAGCATGTT
    ACTTCAACTT CAAATCCACC ACCACCCCCA CCACCACCAC CTCCTCCCCC ACCAGCCCCC
    ATACATCTAG CAAATCAACC TGCGGTTTTA CCCAAATTTT TCACTGGTGG ATTTCCACCT
    CAGAAAGCAC CCAAACCTTC ATGTGGCATT CTTCCAGTAT CCCCAGTAGC TCCAGCACTT
    CAACCTCCAC CACCGCCTCC TCCTCCACCT CCTCCTCCAC CACCTGCACC ATTGAAGAAT
    CAGCCTCCAC CTACACTGCC CAAGCAGCAC AGCCTCTCCA AGACACTGCC CTTCTCCAGT
    CAAACTTCTT CAGTACCGTC TATGGTTAAG CAGCTTGCTA GTCAATTTCC TGTTGCATCT
    CCTGCATTAA CCAATCAAAC AGATAGTCTC AAAGCTCCCC AGTCTCCACC TGCAGTGAAG
    GTTAAGCCAA AATGGCAGCC TGTAGGTCAA GGCCAGCAGC AACAGAGGTC ACCTGAATTT
    CCACCACCTC CACCAGACAA TGCCCTTGGC TTTGCTTCAC CAACACCTCC ACCACCACCA
    CCTCCTCCTC TTCCTCCAGG TCCTACTCCT CCTCCTCCCC CTCCTCCTCC TCCTCTGATG
    TCAGGCTCCC CTATCAAAAA GTCTCCCTCT GGCTCATCTG CAGGTGTTAA GAAACCACCG
    CCAACTCCTC AGCGCAACTC CAGTGTGAAG TACGATGCCT CAACGGAATA CCAGGAGTCC
    CGGAAGAACT TGGTGAGCAA GTTTAACCCA AGTTCTGCTT CATCAACAAC ATCAACCTCC
    AGCTGTTCAC CCTCAAAGGA GCTCTCTGCA GGCCCTCAAG CACCCCCCAA ACCAGGGAAG
    CTCAATCTGG CAAATCTTCC TTTGGCTTTG CAGAACAAAT TCAATCAGAA CCGCCAGTCA
    GCGGCAGACT TTCCATCTCC TCCACCCCCA GAAAATGATT TTTTCCCACC TCCGCCAATA
    GAGTCCGACC TCCCTCCACC ACCACCTCTT CCAGGGGATC TAAATGGTGT GTCTCCCAAA
    GTGGCCGTAG TCAATCCTCA GCCTCAGCCT GCTTGGGGCA AAAGTTCCCT AAAGAAGACA
    CAACCTCCAG CATCACTTCG CTGTAATAAC ACTGTTAGAG AGTCTCCACC ACTCTCACCA
    CCTTCCATAC ACGGGGGGCC AATTCCCCAT TCTCCCAAAG GCACCACACA GCCAAACTTT
    TTGGAGGACC TTAACAAGAC ACTGAAACGC AAGTCTTCAA GAGTCTCTGG AGACAAAGTG
    GACCCTGTGG CCACAATGGA TGACATGGTT TTGCCACCAC CTCCCCCTGA ACTTCTGCAG
    GACCAGCAGA GGCTCAGTGG CAGTGGTTTC ATGTCCGGGA ACATCTCAGG CTATGCAACA
    CTACGGCGAG GGCCTCCTCC TGCCCCACCC AAGCGGGACC ACAGTACCAA ACTTACAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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