TMTC4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Gene Symbol	TMTC4
Entrez Gene ID	560605
Full Name	transmembrane and tetratricopeptide repeat containing 4
Synonyms	si:ch211-168k14.1,wu:fc58d04
General protein information	Preferred Names transmembrane and tetratricopeptide repeat containing 4 Names transmembrane and TPR repeat-containing protein 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001173502.1	NP_001166973.1	transmembrane and TPR repeat-containing protein 4
XM_009304747.3	XP_009303022.1	transmembrane and TPR repeat-containing protein 4 isoform X1
XM_021478692.1	XP_021334367.1	transmembrane and TPR repeat-containing protein 4 isoform X1
XM_009304747.2	XP_009303022.1	transmembrane and TPR repeat-containing protein 4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMTC4	NP_116202.2
Pan troglodytes (chimpanzee)	TMTC4	XP_509717.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003363	XP_314265.4
Caenorhabditis elegans (roundworm)	F38B6.6	NP_509123.2
Rattus norvegicus (Norway rat)	Tmtc4	NP_001127886.1
Gallus gallus (chicken)	TMTC4	XP_416968.4
Mus musculus (house mouse)	Tmtc4	NP_082927.1
Danio rerio (zebrafish)	tmtc4	NP_001166973.1
Drosophila melanogaster (fruit fly)	CG5038	NP_650451.1
Xenopus tropicalis (tropical clawed frog)	tmtc4	NP_001121486.1
Canis lupus familiaris (dog)	TMTC4	XP_005634114.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMTC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMTC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32572	NM_001173502.1 Latest version!	Danio rerio transmembrane O-mannosyltransferase targeting cadherins 4 (tmtc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa32572	XM_021478692.1 Latest version!	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa32572	XM_009304747.3 Latest version!	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa32572	XM_009304747.2	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMTC4 ( NM_001173502.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001166973.1 Danio rerio transmembrane O-mannosyltransferase targeting cadherins 4 (tmtc4), mRNA.

Price & Availability↑

CloneID	ODa32572
Clone ID Related Accession (Same CDS sequence)	NM_001173502.1 , XM_009304747.3 , XM_021478692.1 , XM_009304747.2
Accession Version	NM_001173502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Danio rerio(zebrafish)
Product	transmembrane and TPR repeat-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571700.8, CT627906.2, EB786153.1 and BC133908.1. On May 20, 2010 this sequence version replaced XM_684003.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01021254.1, GFIL01041342.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166973.1
CDS	487..2706
Translation	MVLSEPYWDHHIPLPKLGPLHSRLTTAFVALICFINSYDGDFVFDDSEAIINNKDLNPDTPLSNIWKNDFWGSNLSSNSSHKSYRPLTVLTFRINYLLAGGLHPVGFHVLNVALHCVISVLMIDVFAILIGGPVQDGKGAKLSLPPKASFLAALFFAAHPVHTESVAGIVGRADLLCALFFQLSFLVYCKAFQVGGDKKFSVLWIFGSILLCAVAMLCKEQGITALGVNAAYDILMVCNLNIYELAHRLLSRRKSADIGGLPRNGLLMRLALLFLGGSLLMYTRWKIMGTGPPSFTEVDNPASFAENIILRIVNYNYYYSLNAWLLLCPWWLCFDWSMGCVPLISSAADWRIVWPAVLWFCLIGLVSQALFSQDSNKRRTLTFGLVLLVIPFLPASNVFFRVGFVIAERVLYLSSAGYCLILAYAVGYCSCHWRKHKRLLRAATLTLLCVNVTRSAQRSQQWRSEQSLFTSALSVCPLNAKVHYNVGKNLADRGNQSAAVQYYREAVRLHPKYVHAMNNLGNILKERNELKEAEELLSAAVHIQPDFAAAWMNLGIVQNSLKKFNKAEQSYWNAIRFRKKYPDCYYNLGRLYADLNRSIDALNAWRNATMLKPDHSLAWNNMVILLDNTGNLAQAELIGKEALKILPKDHTIMFSLANVLGKQEKYKESEGFFLNALKINPNVASCHGNLAVLYHRWGKLDLAKRHYELSLRLDPSAPGTKENYNMLKRKLEQRQRTVG

Target ORF information:

RefSeq Version	NM_001173502.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane O-mannosyltransferase targeting cadherins 4 (tmtc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173502.1

ORF Insert Sequence:

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMTC4 ( XM_009304747.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303022.1 Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa32572
Clone ID Related Accession (Same CDS sequence)	NM_001173502.1 , XM_009304747.3 , XM_021478692.1 , XM_009304747.2
Accession Version	XM_009304747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane and TPR repeat-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303022.1
CDS	400..2619
Translation	MVLSEPYWDHHIPLPKLGPLHSRLTTAFVALICFINSYDGDFVFDDSEAIINNKDLNPDTPLSNIWKNDFWGSNLSSNSSHKSYRPLTVLTFRINYLLAGGLHPVGFHVLNVALHCVISVLMIDVFAILIGGPVQDGKGAKLSLPPKASFLAALFFAAHPVHTESVAGIVGRADLLCALFFQLSFLVYCKAFQVGGDKKFSVLWIFGSILLCAVAMLCKEQGITALGVNAAYDILMVCNLNIYELAHRLLSRRKSADIGGLPRNGLLMRLALLFLGGSLLMYTRWKIMGTGPPSFTEVDNPASFAENIILRIVNYNYYYSLNAWLLLCPWWLCFDWSMGCVPLISSAADWRIVWPAVLWFCLIGLVSQALFSQDSNKRRTLTFGLVLLVIPFLPASNVFFRVGFVIAERVLYLSSAGYCLILAYAVGYCSCHWRKHKRLLRAATLTLLCVNVTRSAQRSQQWRSEQSLFTSALSVCPLNAKVHYNVGKNLADRGNQSAAVQYYREAVRLHPKYVHAMNNLGNILKERNELKEAEELLSAAVHIQPDFAAAWMNLGIVQNSLKKFNKAEQSYWNAIRFRKKYPDCYYNLGRLYADLNRSIDALNAWRNATMLKPDHSLAWNNMVILLDNTGNLAQAELIGKEALKILPKDHTIMFSLANVLGKQEKYKESEGFFLNALKINPNVASCHGNLAVLYHRWGKLDLAKRHYELSLRLDPSAPGTKENYNMLKRKLEQRQRTVG

Target ORF information:

RefSeq Version	XM_009304747.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304747.3

ORF Insert Sequence:

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMTC4 ( XM_021478692.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334367.1 Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X2, mRNA.

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CloneID	ODa32572
Clone ID Related Accession (Same CDS sequence)	NM_001173502.1 , XM_009304747.3 , XM_021478692.1 , XM_009304747.2
Accession Version	XM_021478692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane and TPR repeat-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334367.1
CDS	490..2709
Translation	MVLSEPYWDHHIPLPKLGPLHSRLTTAFVALICFINSYDGDFVFDDSEAIINNKDLNPDTPLSNIWKNDFWGSNLSSNSSHKSYRPLTVLTFRINYLLAGGLHPVGFHVLNVALHCVISVLMIDVFAILIGGPVQDGKGAKLSLPPKASFLAALFFAAHPVHTESVAGIVGRADLLCALFFQLSFLVYCKAFQVGGDKKFSVLWIFGSILLCAVAMLCKEQGITALGVNAAYDILMVCNLNIYELAHRLLSRRKSADIGGLPRNGLLMRLALLFLGGSLLMYTRWKIMGTGPPSFTEVDNPASFAENIILRIVNYNYYYSLNAWLLLCPWWLCFDWSMGCVPLISSAADWRIVWPAVLWFCLIGLVSQALFSQDSNKRRTLTFGLVLLVIPFLPASNVFFRVGFVIAERVLYLSSAGYCLILAYAVGYCSCHWRKHKRLLRAATLTLLCVNVTRSAQRSQQWRSEQSLFTSALSVCPLNAKVHYNVGKNLADRGNQSAAVQYYREAVRLHPKYVHAMNNLGNILKERNELKEAEELLSAAVHIQPDFAAAWMNLGIVQNSLKKFNKAEQSYWNAIRFRKKYPDCYYNLGRLYADLNRSIDALNAWRNATMLKPDHSLAWNNMVILLDNTGNLAQAELIGKEALKILPKDHTIMFSLANVLGKQEKYKESEGFFLNALKINPNVASCHGNLAVLYHRWGKLDLAKRHYELSLRLDPSAPGTKENYNMLKRKLEQRQRTVG

Target ORF information:

RefSeq Version	XM_021478692.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478692.1

ORF Insert Sequence:

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMTC4 ( XM_009304747.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303022.1 Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.

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CloneID	ODa32572
Clone ID Related Accession (Same CDS sequence)	NM_001173502.1 , XM_009304747.3 , XM_021478692.1 , XM_009304747.2
Accession Version	XM_009304747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane and TPR repeat-containing protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303022.1
CDS	386..2605
Translation	MVLSEPYWDHHIPLPKLGPLHSRLTTAFVALICFINSYDGDFVFDDSEAIINNKDLNPDTPLSNIWKNDFWGSNLSSNSSHKSYRPLTVLTFRINYLLAGGLHPVGFHVLNVALHCVISVLMIDVFAILIGGPVQDGKGAKLSLPPKASFLAALFFAAHPVHTESVAGIVGRADLLCALFFQLSFLVYCKAFQVGGDKKFSVLWIFGSILLCAVAMLCKEQGITALGVNAAYDILMVCNLNIYELAHRLLSRRKSADIGGLPRNGLLMRLALLFLGGSLLMYTRWKIMGTGPPSFTEVDNPASFAENIILRIVNYNYYYSLNAWLLLCPWWLCFDWSMGCVPLISSAADWRIVWPAVLWFCLIGLVSQALFSQDSNKRRTLTFGLVLLVIPFLPASNVFFRVGFVIAERVLYLSSAGYCLILAYAVGYCSCHWRKHKRLLRAATLTLLCVNVTRSAQRSQQWRSEQSLFTSALSVCPLNAKVHYNVGKNLADRGNQSAAVQYYREAVRLHPKYVHAMNNLGNILKERNELKEAEELLSAAVHIQPDFAAAWMNLGIVQNSLKKFNKAEQSYWNAIRFRKKYPDCYYNLGRLYADLNRSIDALNAWRNATMLKPDHSLAWNNMVILLDNTGNLAQAELIGKEALKILPKDHTIMFSLANVLGKQEKYKESEGFFLNALKINPNVASCHGNLAVLYHRWGKLDLAKRHYELSLRLDPSAPGTKENYNMLKRKLEQRQRTVG

Target ORF information:

RefSeq Version	XM_009304747.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane and tetratricopeptide repeat containing 4 (tmtc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304747.2

ORF Insert Sequence:

ATGGTGCTTT CTGAGCCTTA CTGGGATCAT CACATCCCCC TGCCAAAACT GGGACCCTTG 
CACTCCCGGC TGACTACTGC CTTTGTTGCC CTGATCTGTT TTATAAACAG CTATGATGGG 
GACTTTGTCT TTGATGATTC AGAAGCCATC ATCAATAACA AGGATTTGAA TCCTGACACT 
CCTCTCAGTA ACATCTGGAA AAATGATTTT TGGGGAAGTA ACTTAAGTAG CAACTCTAGT 
CACAAATCCT ACCGGCCGCT GACCGTCCTG ACATTCAGGA TTAACTACCT CTTGGCTGGG 
GGATTGCATC CAGTTGGCTT TCACGTGCTC AACGTTGCTC TCCACTGTGT AATTTCTGTG 
TTAATGATTG ATGTGTTTGC AATATTAATT GGTGGCCCGG TTCAAGATGG AAAAGGGGCG 
AAACTCAGTC TCCCTCCGAA AGCGTCCTTC CTGGCGGCGC TTTTCTTTGC TGCACACCCA 
GTTCACACAG AAAGTGTGGC TGGCATTGTT GGCAGAGCAG ATTTGTTGTG TGCCCTCTTT 
TTTCAGCTCT CTTTTCTGGT TTACTGCAAA GCTTTTCAAG TTGGTGGCGA TAAAAAGTTC 
TCTGTGTTGT GGATATTTGG CAGCATTCTT CTGTGTGCTG TAGCCATGCT CTGCAAGGAA 
CAAGGCATTA CGGCTTTGGG AGTTAATGCT GCTTATGACA TTCTCATGGT GTGCAATCTG 
AACATTTATG AGCTTGCTCA CCGGCTGCTG TCCAGGAGAA AATCAGCTGA CATTGGTGGC 
TTGCCAAGAA ACGGTTTGCT TATGAGGTTG GCTCTACTCT TTCTTGGTGG TTCCTTACTT 
ATGTACACGA GATGGAAAAT CATGGGAACT GGACCTCCAT CTTTCACCGA GGTGGATAAC 
CCAGCATCCT TTGCAGAAAA TATCATCCTG AGGATTGTGA ACTATAATTA CTACTACTCT 
TTGAATGCCT GGTTGCTGCT GTGTCCCTGG TGGCTGTGTT TTGATTGGTC AATGGGCTGT 
GTGCCTCTGA TTAGCTCAGC TGCTGATTGG AGGATCGTCT GGCCTGCTGT GCTGTGGTTC 
TGTCTGATTG GCTTGGTGAG CCAAGCTCTG TTCTCACAGG ACAGTAATAA AAGAAGAACT 
CTGACATTTG GCTTGGTACT GTTGGTGATC CCCTTCCTGC CAGCTAGTAA TGTGTTCTTC 
AGAGTGGGTT TTGTGATTGC GGAGCGTGTG CTGTACCTCT CCTCAGCGGG CTACTGCCTC 
ATTCTGGCCT ACGCTGTGGG CTACTGTAGC TGCCACTGGA GGAAACACAA GAGGCTGCTG 
AGAGCAGCCA CGTTGACGCT GCTGTGTGTG AATGTGACTC GCTCTGCACA GCGCAGTCAA 
CAGTGGAGGT CTGAACAGAG TCTCTTCACC AGCGCCCTTT CTGTGTGCCC GCTCAACGCC 
AAGGTGCATT ATAATGTTGG CAAAAATCTT GCTGACAGGG GAAACCAGAG TGCTGCAGTC 
CAGTACTACA GGGAGGCAGT CAGATTACAT CCCAAGTACG TTCATGCTAT GAACAATCTA 
GGCAATATAT TAAAGGAAAG AAATGAATTA AAGGAAGCTG AAGAGCTCCT GTCAGCTGCG 
GTTCATATTC AGCCTGATTT TGCTGCAGCA TGGATGAACT TGGGAATTGT GCAGAACAGT 
CTAAAAAAAT TTAACAAAGC TGAACAGAGC TATTGGAACG CCATTCGATT CAGGAAGAAA 
TACCCAGACT GTTATTACAA CTTGGGACGT CTGTATGCAG ACCTGAACCG TAGCATTGAT 
GCGCTAAATG CCTGGAGGAA TGCCACCATG CTGAAGCCTG ACCACAGCTT GGCATGGAAC 
AATATGGTCA TACTGTTAGA CAACACTGGT AATCTGGCAC AAGCTGAGCT TATTGGAAAG 
GAGGCATTAA AGATATTGCC AAAAGATCAC ACCATCATGT TCTCCCTCGC CAATGTCCTT 
GGGAAACAGG AAAAATACAA GGAGTCAGAA GGATTCTTTC TTAACGCTCT GAAGATCAAC 
CCAAACGTTG CCAGTTGCCA TGGTAATCTT GCTGTTCTTT ATCACCGGTG GGGAAAACTA 
GACTTAGCAA AGCGACACTA TGAACTGTCT CTGCGACTGG ATCCCAGCGC TCCAGGGACC 
AAGGAAAACT ACAACATGCT GAAACGCAAG CTGGAACAGC GGCAAAGGAC TGTTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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TMTC4 cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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