anln cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	anln
Entrez Gene ID	562517
Full Name	anillin, actin binding protein
Synonyms	si:ch211-197n10.2,wu:fb74d05,wu:fd10d09,wu:fi41f04
General protein information	Preferred Names anillin, actin binding protein Names anillin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159661.2	XP_005159718.1	actin-binding protein anillin isoform X1
NM_001102676.2	NP_001096146.2	anillin
XM_005159661.3	XP_005159718.1	anillin isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	anln	NP_001096146.2
Xenopus tropicalis (tropical clawed frog)	LOC100491625	XP_002939460.1
Pan troglodytes (chimpanzee)	ANLN	XP_001169875.1
	ANLN	XP_001103129.1
Bos taurus (cattle)	ANLN	XP_002686875.2
Homo sapiens (human)	ANLN	NP_061155.2
Canis lupus familiaris (dog)	ANLN	XP_539518.2
Rattus norvegicus (Norway rat)	Anln	XP_006242776.1
Mus musculus (house mouse)	Anln	NP_082666.2
Gallus gallus (chicken)	ANLN	XP_418836.3

Gene Ontology
Bibliography

Related articles in PubMed

Resolving Heart Regeneration by Replacement Histone Profiling.
Goldman JA, Kuzu G, Lee N, Karasik J, Gemberling M, Foglia MJ, Karra R, Dickson AL, Sun F, Tolstorukov MY, Poss KD
Developmental cell40(4)392-404.e5(2017 02)

The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages.
Cepero Malo M, Duchemin AL, Guglielmi L, Patzel E, Sel S, Auffarth GU, Carl M, Poggi L
PloS one12(1)e0170356(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study provides the in vivo evidence for the requirement of Anillin during asymmetric neurogenic divisions.
Title: Asymmetric inheritance of the apical domain and self-renewal of retinal ganglion cell progenitors depend on Anillin function.

The following anln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the anln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34169	NM_001102676.2 Latest version!	Danio rerio anillin, actin binding protein (anln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24321	XM_005159661.3 Latest version!	Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa24321	XM_005159661.2	Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

anln ( NM_001102676.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001096146.2 Danio rerio anillin, actin binding protein (anln), mRNA.

Price & Availability↑

CloneID	ODa34169
Clone ID Related Accession (Same CDS sequence)	NM_001102676.2
Accession Version	NM_001102676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	anillin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX957241.13. On or before Aug 4, 2009 this sequence version replaced NM_001044987.1, NM_001102676.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC134809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGG TGCCTTTTCT TTGTCCTTTG 
GAGGGGCATG TATATCTAAA AATGCAGTGT GAGGTTGGCT CACGGATAGA GGAAAAAGGC 
TTCCTGACTA TGTTTGAAGA TGTCAGTGGT TTTGGGGCCT GGCACAGGAG GTGGTGCGTC 
CTTTCAGGAT ACTGCATCTC CTATTGGACC TACCCTGATG ATGAAAAACG GAAGAATCCA 
ATGGGCCGCA TTAACCTTGC CAACTGTACC AGTCGTAAAG TGGAGCCGGC AAACCGAGAG 
TTCTGTGCTC GACCCAATAC ATTTGAGCTC ATCACTGTGA GGCCACAGAG AGAGGATGAC 
CGAGAGACCC TGGTCAGCCG GTGCAAAGAC ACACTCTGTG TTACCAAGAA CTGGCTGAGT 
GCTGACACTA AAGACGAGAG GAATCTGTGG ATGCAGAAGC TCAACCAGAT CCTGGTGGAC 
CTGCGCATGT GGCAGCCGGA TTCCTGCTAC AGACCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096146.2
CDS	141..3602
Translation	MDPFTEKLLERTRARRENLQKKMAERPTAANRPMAKRTREPLTDTNSVLSEPTIEKALPSSKPSPFKRRCSDENTFLAGEENKQPVAPQMTDPMTDQKPPLAPTTVRPIPVEQRSAPDMLPTRPPPETEKMVVCPPQSPAKRTDNVLRQKGLDGARETQKEAPTTFPSSMRSRLQRLAGQRQNWDSNGATEAVSESVVVSPLKSHNLDHPPATPTNSEVPVGRKGRLANLAATIGSWEDDLGHPPIRRDNAQAQPGTAYVRPPVSTVTSNSEQLQSARPTSHRPVNRGQQPAVYSPVKSTSVVPPSPQKTEFPQARWAQAVPSPVSSPLKIPPSTQPSSPLKSVTASPSSPHRGYVSQSPLKSHGQVDKLNFGAALNSSPAKPVLAPKPSAADDAVSRVRFTKDATPTQHRGPAATPGGVKSFLERFGERCQERNQNTPSMLGGPGHTSAASTPATPKFKLLQERLGGAQATSTTAALTEKQKMEREAELAQIRNRFQKGNMLRGKEDISEVKDDTENKEIELSLQSQTSLPLHDNKPSVHVPEVFNSPSKSIGKGMSCASSSPAKEIELKVEMPTVEEPEDVQEVDVVREIEMNVDASVNSEVINELFDGVLEESDEDADEDPLNISSMSLLAPLAETVAAVIKSPERKPMTSTPANSFLVKSSTPENPSRPSKFQRTRMLRADSSDSIDVLDEQQNQKLLYSIDAYRSTRVKSEPERPHVKQVIVRKEDVSQRMEMNGSSSHINIKQKMKMLTNEMNLQQTVIHQASQALNCCTDEEHGKGSQVEAEAERLLIIATERRAALKAELDRLKAEGPTAQKKSASSVPVDIGLPASKGSISLLELRLPLKADFICSSANKPECGNHYFFVMIRAGPENTVATPLSSTRTGLSGDALTFTTTFTLSDVSNDFEIDIEVYCLVQKRELNPDKRKKASKSKAITPKRFLNISKSNLQTPVMASPGGPNAVRTSSFVLVGSHKLTLASIGKNKFPLEKVPFLCPLEGHVYLKMQCEVGSRIEEKGFLTMFEDVSGFGAWHRRWCVLSGYCISYWTYPDDEKRKNPMGRINLANCTSRKVEPANREFCARPNTFELITVRPQREDDRETLVSRCKDTLCVTKNWLSADTKDERNLWMQKLNQILVDLRMWQPDSCYRPM

Target ORF information:

RefSeq Version	NM_001102676.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anillin, actin binding protein (anln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102676.2

ORF Insert Sequence:

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGG TGCCTTTTCT TTGTCCTTTG 
GAGGGGCATG TATATCTAAA AATGCAGTGT GAGGTTGGCT CACGGATAGA GGAAAAAGGC 
TTCCTGACTA TGTTTGAAGA TGTCAGTGGT TTTGGGGCCT GGCACAGGAG GTGGTGCGTC 
CTTTCAGGAT ACTGCATCTC CTATTGGACC TACCCTGATG ATGAAAAACG GAAGAATCCA 
ATGGGCCGCA TTAACCTTGC CAACTGTACC AGTCGTAAAG TGGAGCCGGC AAACCGAGAG 
TTCTGTGCTC GACCCAATAC ATTTGAGCTC ATCACTGTGA GGCCACAGAG AGAGGATGAC 
CGAGAGACCC TGGTCAGCCG GTGCAAAGAC ACACTCTGTG TTACCAAGAA CTGGCTGAGT 
GCTGACACTA AAGACGAGAG GAATCTGTGG ATGCAGAAGC TCAACCAGAT CCTGGTGGAC 
CTGCGCATGT GGCAGCCGGA TTCCTGCTAC AGACCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

anln ( XM_005159661.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159718.1 Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24321
Clone ID Related Accession (Same CDS sequence)	XM_005159661.2 , XM_005159661.3
Accession Version	XM_005159661.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	actin-binding protein anillin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGA TCAAATATGA AAGGAGTGAA 
AGAGAGCTGC TCAGTGACAT GTTTCACAAT AAGGTGCCTT TTCTTTGTCC TTTGGAGGGG 
CATGTATATC TAAAAATGCA GTGTGAGGTT GGCTCACGGA TAGAGGAAAA AGGCTTCCTG 
ACTATGTTTG AAGATGTCAG TGGTTTTGGG GCCTGGCACA GGAGGTGGTG CGTCCTTTCA 
GGATACTGCA TCTCCTATTG GACCTACCCT GATGATGAAA AACGGAAGAA TCCAATGGGC 
CGCATTAACC TTGCCAACTG TACCAGTCGT AAAGTGGAGC CGGCAAACCG AGAGTTCTGT 
GCTCGACCCA ATACATTTGA GCTCATCACT GTGAGGCCAC AGAGAGAGGA TGACCGAGAG 
ACCCTGGTCA GCCGGTGCAA AGACACACTC TGTGTTACCA AGAACTGGCT GAGTGCTGAC 
ACTAAAGACG AGAGGAATCT GTGGATGCAG AAGCTCAACC AGATCCTGGT GGACCTGCGC 
ATGTGGCAGC CGGATTCCTG CTACAGACCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159718.1
CDS	196..3711
Translation	MDPFTEKLLERTRARRENLQKKMAERPTAANRPMAKRTREPLTDTNSVLSEPTIEKALPSSKPSPFKRRCSDENTFLAGEENKQPVAPQMTDPMTDQKPPLAPTTVRPIPVEQRSAPDMLPTRPPPETEKMVVCPPQSPAKRTDNVLRQKGLDGARETQKEAPTTFPSSMRSRLQRLAGQRQNWDSNGATEAVSESVVVSPLKSHNLDHPPATPTNSEVPVGRKGRLANLAATIGSWEDDLGHPPIRRDNAQAQPGTAYVRPPVSTVTSNSEQLQSARPTSHRPVNRGQQPAVYSPVKSTSVVPPSPQKTEFPQARWAQAVPSPVSSPLKIPPSTQPSSPLKSVTASPSSPHRGYVSQSPLKSHGQVDKLNFGAALNSSPAKPVLAPKPSAADDAVSRVRFTKDATPTQHRGPAATPGGVKSFLERFGERCQERNQNTPSMLGGPGHTSAASTPATPKFKLLQERLGGAQATSTTAALTEKQKMEREAELAQIRNRFQKGNMLRGKEDISEVKDDTENKEIELSLQSQTSLPLHDNKPSVHVPEVFNSPSKSIGKGMSCASSSPAKEIELKVEMPTVEEPEDVQEVDVVREIEMNVDASVNSEVINELFDGVLEESDEDADEDPLNISSMSLLAPLAETVAAVIKSPERKPMTSTPANSFLVKSSTPENPSRPSKFQRTRMLRADSSDSIDVLDEQQNQKLLYSIDAYRSTRVKSEPERPHVKQVIVRKEDVSQRMEMNGSSSHINIKQKMKMLTNEMNLQQTVIHQASQALNCCTDEEHGKGSQVEAEAERLLIIATERRAALKAELDRLKAEGPTAQKKSASSVPVDIGLPASKGSISLLELRLPLKADFICSSANKPECGNHYFFVMIRAGPENTVATPLSSTRTGLSGDALTFTTTFTLSDVSNDFEIDIEVYCLVQKRELNPDKRKKASKSKAITPKRFLNISKSNLQTPVMASPGGPNAVRTSSFVLVGSHKLTLASIGKNKFPLEKIKYERSERELLSDMFHNKVPFLCPLEGHVYLKMQCEVGSRIEEKGFLTMFEDVSGFGAWHRRWCVLSGYCISYWTYPDDEKRKNPMGRINLANCTSRKVEPANREFCARPNTFELITVRPQREDDRETLVSRCKDTLCVTKNWLSADTKDERNLWMQKLNQILVDLRMWQPDSCYRPM

Target ORF information:

RefSeq Version	XM_005159661.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159661.2

ORF Insert Sequence:

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGA TCAAATATGA AAGGAGTGAA 
AGAGAGCTGC TCAGTGACAT GTTTCACAAT AAGGTGCCTT TTCTTTGTCC TTTGGAGGGG 
CATGTATATC TAAAAATGCA GTGTGAGGTT GGCTCACGGA TAGAGGAAAA AGGCTTCCTG 
ACTATGTTTG AAGATGTCAG TGGTTTTGGG GCCTGGCACA GGAGGTGGTG CGTCCTTTCA 
GGATACTGCA TCTCCTATTG GACCTACCCT GATGATGAAA AACGGAAGAA TCCAATGGGC 
CGCATTAACC TTGCCAACTG TACCAGTCGT AAAGTGGAGC CGGCAAACCG AGAGTTCTGT 
GCTCGACCCA ATACATTTGA GCTCATCACT GTGAGGCCAC AGAGAGAGGA TGACCGAGAG 
ACCCTGGTCA GCCGGTGCAA AGACACACTC TGTGTTACCA AGAACTGGCT GAGTGCTGAC 
ACTAAAGACG AGAGGAATCT GTGGATGCAG AAGCTCAACC AGATCCTGGT GGACCTGCGC 
ATGTGGCAGC CGGATTCCTG CTACAGACCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

anln ( XM_005159661.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159718.1 Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.

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CloneID	ODa24321
Clone ID Related Accession (Same CDS sequence)	XM_005159661.2 , XM_005159661.3
Accession Version	XM_005159661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGA TCAAATATGA AAGGAGTGAA 
AGAGAGCTGC TCAGTGACAT GTTTCACAAT AAGGTGCCTT TTCTTTGTCC TTTGGAGGGG 
CATGTATATC TAAAAATGCA GTGTGAGGTT GGCTCACGGA TAGAGGAAAA AGGCTTCCTG 
ACTATGTTTG AAGATGTCAG TGGTTTTGGG GCCTGGCACA GGAGGTGGTG CGTCCTTTCA 
GGATACTGCA TCTCCTATTG GACCTACCCT GATGATGAAA AACGGAAGAA TCCAATGGGC 
CGCATTAACC TTGCCAACTG TACCAGTCGT AAAGTGGAGC CGGCAAACCG AGAGTTCTGT 
GCTCGACCCA ATACATTTGA GCTCATCACT GTGAGGCCAC AGAGAGAGGA TGACCGAGAG 
ACCCTGGTCA GCCGGTGCAA AGACACACTC TGTGTTACCA AGAACTGGCT GAGTGCTGAC 
ACTAAAGACG AGAGGAATCT GTGGATGCAG AAGCTCAACC AGATCCTGGT GGACCTGCGC 
ATGTGGCAGC CGGATTCCTG CTACAGACCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159718.1
CDS	156..3671
Translation	MDPFTEKLLERTRARRENLQKKMAERPTAANRPMAKRTREPLTDTNSVLSEPTIEKALPSSKPSPFKRRCSDENTFLAGEENKQPVAPQMTDPMTDQKPPLAPTTVRPIPVEQRSAPDMLPTRPPPETEKMVVCPPQSPAKRTDNVLRQKGLDGARETQKEAPTTFPSSMRSRLQRLAGQRQNWDSNGATEAVSESVVVSPLKSHNLDHPPATPTNSEVPVGRKGRLANLAATIGSWEDDLGHPPIRRDNAQAQPGTAYVRPPVSTVTSNSEQLQSARPTSHRPVNRGQQPAVYSPVKSTSVVPPSPQKTEFPQARWAQAVPSPVSSPLKIPPSTQPSSPLKSVTASPSSPHRGYVSQSPLKSHGQVDKLNFGAALNSSPAKPVLAPKPSAADDAVSRVRFTKDATPTQHRGPAATPGGVKSFLERFGERCQERNQNTPSMLGGPGHTSAASTPATPKFKLLQERLGGAQATSTTAALTEKQKMEREAELAQIRNRFQKGNMLRGKEDISEVKDDTENKEIELSLQSQTSLPLHDNKPSVHVPEVFNSPSKSIGKGMSCASSSPAKEIELKVEMPTVEEPEDVQEVDVVREIEMNVDASVNSEVINELFDGVLEESDEDADEDPLNISSMSLLAPLAETVAAVIKSPERKPMTSTPANSFLVKSSTPENPSRPSKFQRTRMLRADSSDSIDVLDEQQNQKLLYSIDAYRSTRVKSEPERPHVKQVIVRKEDVSQRMEMNGSSSHINIKQKMKMLTNEMNLQQTVIHQASQALNCCTDEEHGKGSQVEAEAERLLIIATERRAALKAELDRLKAEGPTAQKKSASSVPVDIGLPASKGSISLLELRLPLKADFICSSANKPECGNHYFFVMIRAGPENTVATPLSSTRTGLSGDALTFTTTFTLSDVSNDFEIDIEVYCLVQKRELNPDKRKKASKSKAITPKRFLNISKSNLQTPVMASPGGPNAVRTSSFVLVGSHKLTLASIGKNKFPLEKIKYERSERELLSDMFHNKVPFLCPLEGHVYLKMQCEVGSRIEEKGFLTMFEDVSGFGAWHRRWCVLSGYCISYWTYPDDEKRKNPMGRINLANCTSRKVEPANREFCARPNTFELITVRPQREDDRETLVSRCKDTLCVTKNWLSADTKDERNLWMQKLNQILVDLRMWQPDSCYRPM

Target ORF information:

RefSeq Version	XM_005159661.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anillin, actin binding protein (anln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159661.3

ORF Insert Sequence:

ATGGATCCAT TCACCGAGAA ACTGTTGGAG CGCACACGTG CGCGTAGAGA AAATCTGCAG 
AAGAAGATGG CGGAGAGACC AACGGCAGCC AACCGTCCAA TGGCTAAAAG AACAAGAGAG 
CCACTTACAG ATACAAACAG TGTCTTAAGT GAGCCGACTA TTGAAAAAGC GCTTCCCTCC 
TCCAAGCCAT CCCCCTTCAA ACGCCGCTGT TCAGACGAAA ATACTTTTCT TGCTGGAGAA 
GAAAACAAGC AGCCGGTGGC CCCACAAATG ACAGACCCCA TGACTGACCA GAAACCACCC 
CTGGCACCAA CCACTGTTAG GCCGATACCT GTGGAACAAA GGTCTGCTCC AGATATGCTG 
CCCACTCGGC CTCCTCCTGA GACTGAAAAG ATGGTGGTGT GTCCTCCACA GTCTCCAGCA 
AAACGCACTG ATAATGTATT GAGACAAAAA GGTCTAGATG GAGCTAGAGA GACGCAGAAA 
GAAGCACCAA CCACATTTCC TTCCAGCATG AGATCTCGTC TGCAGAGACT GGCTGGTCAG 
AGGCAGAACT GGGACTCTAA TGGGGCCACA GAAGCTGTCT CAGAGAGTGT AGTTGTATCT 
CCTTTGAAGT CCCATAACTT GGATCACCCT CCTGCTACTC CAACAAATTC TGAGGTTCCT 
GTTGGAAGAA AGGGTAGACT TGCAAATCTT GCTGCCACAA TCGGCTCTTG GGAAGATGAC 
CTTGGACACC CGCCCATTCG CCGGGACAAT GCACAAGCAC AGCCTGGCAC GGCCTATGTG 
CGCCCACCAG TCAGTACAGT AACAAGTAAC AGTGAGCAAC TTCAGTCTGC CCGGCCAACT 
TCACACAGGC CTGTTAATAG AGGCCAGCAG CCTGCAGTGT ATTCTCCAGT CAAATCAACC 
AGTGTGGTTC CTCCTAGTCC TCAAAAGACA GAGTTTCCTC AGGCCAGATG GGCTCAAGCA 
GTGCCTTCTC CTGTTTCCAG CCCACTGAAG ATTCCCCCTT CCACCCAGCC CTCCAGTCCA 
CTCAAATCTG TGACGGCATC TCCCTCTAGT CCCCACAGAG GTTACGTCTC TCAGAGCCCA 
CTGAAGAGCC ATGGCCAAGT TGACAAGTTG AATTTTGGAG CAGCACTTAA TTCAAGTCCT 
GCGAAACCAG TTTTGGCACC TAAACCATCT GCCGCTGATG ATGCTGTGAG TCGAGTGAGG 
TTTACTAAAG ACGCAACACC CACACAGCAC AGAGGCCCAG CTGCAACGCC TGGAGGGGTC 
AAGTCATTTT TGGAACGTTT TGGAGAAAGG TGTCAGGAGC GTAACCAGAA CACTCCCTCC 
ATGTTAGGAG GTCCAGGCCA CACATCTGCA GCTTCTACTC CTGCTACCCC CAAATTTAAA 
CTGCTCCAGG AGAGGCTTGG AGGGGCTCAG GCCACTTCCA CCACAGCCGC CCTCACTGAG 
AAGCAGAAAA TGGAGCGAGA AGCAGAACTG GCACAAATCC GTAATAGGTT TCAGAAAGGC 
AACATGTTGA GAGGCAAAGA GGACATCAGT GAGGTCAAGG ATGACACTGA AAATAAGGAA 
ATTGAACTTT CTCTACAGAG CCAGACTTCC TTACCACTGC ATGATAATAA GCCTTCTGTA 
CATGTGCCTG AAGTATTCAA CAGCCCCTCT AAATCTATTG GAAAAGGCAT GAGCTGTGCT 
TCTTCAAGTC CAGCAAAAGA AATTGAACTA AAAGTGGAGA TGCCTACAGT GGAAGAGCCA 
GAGGATGTCC AAGAAGTTGA TGTAGTGCGT GAGATTGAGA TGAACGTGGA CGCCTCTGTT 
AACTCTGAGG TGATAAATGA ACTGTTTGAT GGAGTTCTGG AGGAAAGTGA TGAGGATGCA 
GATGAAGATC CTCTGAATAT CTCCTCCATG TCTCTGCTTG CCCCGCTTGC AGAGACTGTA 
GCAGCTGTGA TCAAGAGCCC AGAAAGAAAG CCTATGACAT CAACCCCTGC CAACTCATTT 
TTGGTGAAGA GTTCCACCCC TGAGAATCCA TCCAGGCCCA GTAAGTTCCA GAGGACACGC 
ATGTTACGAG CTGATTCATC TGACAGCATT GATGTTCTAG ATGAGCAACA AAACCAGAAA 
CTTCTCTATA GCATTGATGC CTACAGATCC ACCAGGGTTA AGAGCGAGCC AGAGAGACCT 
CATGTTAAAC AGGTGATTGT CAGGAAAGAG GATGTGTCTC AGAGAATGGA GATGAATGGG 
AGCTCCAGTC ACATCAACAT CAAGCAGAAA ATGAAGATGT TGACCAATGA GATGAACCTG 
CAGCAGACCG TGATCCATCA GGCTAGTCAG GCTCTTAACT GCTGCACAGA TGAAGAACAT 
GGAAAAGGAT CTCAGGTGGA GGCTGAGGCT GAAAGACTGC TGATTATTGC CACCGAGAGG 
AGGGCCGCTC TTAAGGCTGA GCTGGACCGA TTAAAAGCAG AAGGACCAAC AGCACAAAAG 
AAGAGCGCAA GCAGCGTCCC GGTGGATATT GGTTTACCTG CTTCTAAAGG CTCCATCTCA 
CTACTGGAGC TGAGACTTCC ACTGAAGGCC GACTTTATCT GCTCTTCTGC CAACAAGCCT 
GAGTGTGGAA ACCATTACTT CTTTGTGATG ATCCGTGCGG GACCTGAGAA CACTGTGGCA 
ACTCCTCTAT CAAGCACACG CACGGGTCTG AGTGGTGATG CTTTGACCTT CACCACCACA 
TTCACTTTGT CTGATGTGTC TAATGACTTT GAGATTGATA TTGAAGTCTA CTGTTTGGTT 
CAGAAACGAG AGCTGAACCC TGACAAGAGG AAAAAGGCCA GCAAGTCAAA GGCAATCACA 
CCAAAAAGGT TCCTCAATAT CTCTAAGAGT AACCTCCAAA CACCTGTGAT GGCCAGCCCT 
GGTGGTCCAA ATGCAGTGCG CACCAGTAGC TTTGTACTTG TTGGATCACA CAAGTTAACT 
CTGGCTTCTA TTGGGAAAAA CAAGTTCCCT TTGGAGAAGA TCAAATATGA AAGGAGTGAA 
AGAGAGCTGC TCAGTGACAT GTTTCACAAT AAGGTGCCTT TTCTTTGTCC TTTGGAGGGG 
CATGTATATC TAAAAATGCA GTGTGAGGTT GGCTCACGGA TAGAGGAAAA AGGCTTCCTG 
ACTATGTTTG AAGATGTCAG TGGTTTTGGG GCCTGGCACA GGAGGTGGTG CGTCCTTTCA 
GGATACTGCA TCTCCTATTG GACCTACCCT GATGATGAAA AACGGAAGAA TCCAATGGGC 
CGCATTAACC TTGCCAACTG TACCAGTCGT AAAGTGGAGC CGGCAAACCG AGAGTTCTGT 
GCTCGACCCA ATACATTTGA GCTCATCACT GTGAGGCCAC AGAGAGAGGA TGACCGAGAG 
ACCCTGGTCA GCCGGTGCAA AGACACACTC TGTGTTACCA AGAACTGGCT GAGTGCTGAC 
ACTAAAGACG AGAGGAATCT GTGGATGCAG AAGCTCAACC AGATCCTGGT GGACCTGCGC 
ATGTGGCAGC CGGATTCCTG CTACAGACCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resolving Heart Regeneration by Replacement Histone Profiling.
Developmental cell40(4)392-404.e5(2017 02)
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