si:dkey-33b17.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:dkey-33b17.3
Entrez Gene ID	563286
Full Name	si:dkey-33b17.3
Synonyms	si:busm1-160c18.3,si:dz160c18.3,zgc:158775
General protein information	Preferred Names si:dkey-33b17.3 Names uncharacterized protein LOC563286 MHC class II beta chain
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304129.2	XP_009302404.1	uncharacterized protein LOC563286 isoform X1
NM_001080633.2	NP_001074102.2	uncharacterized protein LOC563286

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100331463	XP_002667912.2
Danio rerio (zebrafish)	mhc2dbb	NP_001070245.1
Danio rerio (zebrafish)	mhc2dcb	NP_571781.1
Danio rerio (zebrafish)	si:busm1-104n07.3	XP_005167291.1
Danio rerio (zebrafish)	si:zfos-2070c2.3	XP_002663212.1
Danio rerio (zebrafish)	mhc2dab	NP_571551.2
Danio rerio (zebrafish)	wu:fd58b11	XP_005168179.1
Danio rerio (zebrafish)	si:dkey-33b17.3	XP_005165041.1
Danio rerio (zebrafish)	si:busm1-228j01.6	NP_001001595.1
Danio rerio (zebrafish)	si:busm1-194e12.12	NP_001007207.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates.
Dijkstra JM, Grimholt U, Leong J, Koop BF, Hashimoto K
BMC evolutionary biology13260(2013 Nov)

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
Yang D, Liu Q, Yang M, Wu H, Wang Q, Xiao J, Zhang Y
BMC genomics13319(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following si:dkey-33b17.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-33b17.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa44028	NM_001080633.2 Latest version!	Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa44029	XM_009304129.2 Latest version!	Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:dkey-33b17.3 ( NM_001080633.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074102.2 Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), mRNA.

Price & Availability↑

CloneID	ODa44028
Clone ID Related Accession (Same CDS sequence)	NM_001080633.2
Accession Version	NM_001080633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC563286
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT583723.7. On or before Jun 4, 2016 this sequence version replaced XM_009304128.1, NM_001080633.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC129443.1, GFIL01035288.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGCA GCATTCACTT TGTATTCAGC GTGGCTAAAA TGTCACAGAA AAAGCTCTAT 
AGTTCTCTCA TATTGATTTT GGCTCTATTC TTTGTAGGGG AAAGTGCTAA TGTATATTAC 
ATGTACCGTG TATCAAGATG CATCTTCAGC TCCTCTAATA TCAGTGCCAT GGTGTATTTT 
GACCGCACGT ATTTCAATAA AAATCTATTC ATACAGTTTA ACAGCAATCT GGGGAGGTTT 
GAAGGGTTTA ACGAGTATGG ATTAAAACTT GCAGAGTTCT GGAACAATGG AACATTTGTA 
GATCAGGAGA AAGACGTTGT GGAATTTTTC TGCAAGTATA ATTCTCAAAT CTATGAAAAC 
AGCATCTTAG ATAAAGCAGT GAAACCAAAG GTTAAGCTTA GTTCAGTGAC CCGGGCCGGT 
GGAAGACAAT CAACAGTTTT GATGTGCAGC GCATATGATT TTTATCCTCC ACACATCAAC 
GTGTTCTGGT TGAGAAATGG TGAAGTAATG ACCTCTGAAG TGACCTCAAC TATGGAGATG 
GCTGATGGTG ACTGGTATTA CCAGATTCAC TCTGAGCTTG AATACAGTCC GAAACCTGGA 
GAAAGGATTT CCTGTGTGAT TGAACATGCC AGCTCTAACA AACCCATGAT TTATGACTGG 
GATCCATCTC TTCCTGTCTT TGAGAGGAAT AAAATTTCCA TCGGGGTTTC TGCTCTTGTG 
CTGGGAATCA TCACTGCAGC AGCTGGAATC ATTTACTACA AGAAGAAAAC TACAGGGAGG 
ACTTCAGTAG TTCCAAGTCG CCTGATTACA TTGCCACTTA TGTTCGGACG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074102.2
CDS	10..843
Translation	MFSSIHFVFSVAKMSQKKLYSSLILILALFFVGESANVYYMYRVSRCIFSSSNISAMVYFDRTYFNKNLFIQFNSNLGRFEGFNEYGLKLAEFWNNGTFVDQEKDVVEFFCKYNSQIYENSILDKAVKPKVKLSSVTRAGGRQSTVLMCSAYDFYPPHINVFWLRNGEVMTSEVTSTMEMADGDWYYQIHSELEYSPKPGERISCVIEHASSNKPMIYDWDPSLPVFERNKISIGVSALVLGIITAAAGIIYYKKKTTGRTSVVPSRLITLPLMFGR

Target ORF information:

RefSeq Version	NM_001080633.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080633.2

ORF Insert Sequence:

ATGTTCAGCA GCATTCACTT TGTATTCAGC GTGGCTAAAA TGTCACAGAA AAAGCTCTAT 
AGTTCTCTCA TATTGATTTT GGCTCTATTC TTTGTAGGGG AAAGTGCTAA TGTATATTAC 
ATGTACCGTG TATCAAGATG CATCTTCAGC TCCTCTAATA TCAGTGCCAT GGTGTATTTT 
GACCGCACGT ATTTCAATAA AAATCTATTC ATACAGTTTA ACAGCAATCT GGGGAGGTTT 
GAAGGGTTTA ACGAGTATGG ATTAAAACTT GCAGAGTTCT GGAACAATGG AACATTTGTA 
GATCAGGAGA AAGACGTTGT GGAATTTTTC TGCAAGTATA ATTCTCAAAT CTATGAAAAC 
AGCATCTTAG ATAAAGCAGT GAAACCAAAG GTTAAGCTTA GTTCAGTGAC CCGGGCCGGT 
GGAAGACAAT CAACAGTTTT GATGTGCAGC GCATATGATT TTTATCCTCC ACACATCAAC 
GTGTTCTGGT TGAGAAATGG TGAAGTAATG ACCTCTGAAG TGACCTCAAC TATGGAGATG 
GCTGATGGTG ACTGGTATTA CCAGATTCAC TCTGAGCTTG AATACAGTCC GAAACCTGGA 
GAAAGGATTT CCTGTGTGAT TGAACATGCC AGCTCTAACA AACCCATGAT TTATGACTGG 
GATCCATCTC TTCCTGTCTT TGAGAGGAAT AAAATTTCCA TCGGGGTTTC TGCTCTTGTG 
CTGGGAATCA TCACTGCAGC AGCTGGAATC ATTTACTACA AGAAGAAAAC TACAGGGAGG 
ACTTCAGTAG TTCCAAGTCG CCTGATTACA TTGCCACTTA TGTTCGGACG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-33b17.3 ( XM_009304129.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302404.1 Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44029
Clone ID Related Accession (Same CDS sequence)	XM_009304129.2
Accession Version	XM_009304129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC563286 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA GCATTCACTT TGTATTCAGC GTGGCTAAAA TGTCACAGAA AAAGCTCTAT 
AGTTCTCTCA TATTGATTTT GGCTCTATTC TTTGTAGGGG AAAGTGTGAA ACCAAAGGTT 
AAGCTTAGTT CAGTGACCCG GGCCGGTGGA AGACAATCAA CAGTTTTGAT GTGCAGCGCA 
TATGATTTTT ATCCTCCACA CATCAACGTG TTCTGGTTGA GAAATGGTGA AGTAATGACC 
TCTGAAGTGA CCTCAACTAT GGAGATGGCT GATGGTGACT GGTATTACCA GATTCACTCT 
GAGCTTGAAT ACAGTCCGAA ACCTGGAGAA AGGATTTCCT GTGTGATTGA ACATGCCAGC 
TCTAACAAAC CCATGATTTA TGACTGGGAT CCATCTCTTC CTGTCTTTGA GAGGAATAAA 
ATTTCCATCG GGGTTTCTGC TCTTGTGCTG GGAATCATCA CTGCAGCAGC TGGAATCATT 
TACTACAAGA AGAAAACTAC AGGGAGGACT TCAGTAGTTC CAAGTCGCCT GATTACATTG 
CCACTTATGT TCGGACGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302404.1
CDS	10..570
Translation	MFSSIHFVFSVAKMSQKKLYSSLILILALFFVGESVKPKVKLSSVTRAGGRQSTVLMCSAYDFYPPHINVFWLRNGEVMTSEVTSTMEMADGDWYYQIHSELEYSPKPGERISCVIEHASSNKPMIYDWDPSLPVFERNKISIGVSALVLGIITAAAGIIYYKKKTTGRTSVVPSRLITLPLMFGR

Target ORF information:

RefSeq Version	XM_009304129.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-33b17.3 (si:dkey-33b17.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304129.2

ORF Insert Sequence:

ATGTTCAGCA GCATTCACTT TGTATTCAGC GTGGCTAAAA TGTCACAGAA AAAGCTCTAT 
AGTTCTCTCA TATTGATTTT GGCTCTATTC TTTGTAGGGG AAAGTGTGAA ACCAAAGGTT 
AAGCTTAGTT CAGTGACCCG GGCCGGTGGA AGACAATCAA CAGTTTTGAT GTGCAGCGCA 
TATGATTTTT ATCCTCCACA CATCAACGTG TTCTGGTTGA GAAATGGTGA AGTAATGACC 
TCTGAAGTGA CCTCAACTAT GGAGATGGCT GATGGTGACT GGTATTACCA GATTCACTCT 
GAGCTTGAAT ACAGTCCGAA ACCTGGAGAA AGGATTTCCT GTGTGATTGA ACATGCCAGC 
TCTAACAAAC CCATGATTTA TGACTGGGAT CCATCTCTTC CTGTCTTTGA GAGGAATAAA 
ATTTCCATCG GGGTTTCTGC TCTTGTGCTG GGAATCATCA CTGCAGCAGC TGGAATCATT 
TACTACAAGA AGAAAACTAC AGGGAGGACT TCAGTAGTTC CAAGTCGCCT GATTACATTG 
CCACTTATGT TCGGACGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates.
BMC evolutionary biology13260(2013 Nov)
Dijkstra JM,Grimholt U,Leong J,Koop BF,Hashimoto K

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
BMC genomics13319(2012 Jul)
Yang D,Liu Q,Yang M,Wu H,Wang Q,Xiao J,Zhang Y