cacna1g cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following cacna1g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa54517 XM_017358535.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa54518 XM_017358536.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148360 XM_021480344.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148361 XM_021480345.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148362 XM_017358537.2
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148363 XM_021480346.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148364 XM_021480347.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148365 XM_017358535.2
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148366 XM_017358536.2
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148367 XM_021480348.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa148368 XM_021480349.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa54519 XM_017358537.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa54517
    Clone ID Related Accession (Same CDS sequence) XM_017358535.1
    Accession Version XM_017358535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT
    GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG
    CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA
    GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA
    GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC
    AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC
    TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT
    GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA
    GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG
    GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC
    CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG
    CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT
    TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG
    GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC
    TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC
    ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC
    GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC
    AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC
    TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG
    GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC
    CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC
    AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA
    AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT
    TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA
    GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA
    CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC
    GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG
    CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC
    GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA
    CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAATAT AATGCTGACC
    GGTGTCAGTT GGTCCAGTCC TGATCACACA CAAGAAGCCC AGAGTAAACC CTTTTACTCC
    GATTACTGCC CAACACGACG TCTCATCTAC AACATGTGCA AGAGCCAGTA TCTGGACCTG
    TTCATCACCA TCGTCATCGC CCTCAATGTC ATCACCATGT CTATGGAGCA CTACCACCAA
    CCAAAGGTTT TGAGCGATTC CCTAAAGATC TGTAACTACA TCTTCACAGT CATATTTGTG
    CTGGAGTCCA TCTTCAAACT TGTTGCTTTT GGATTTCGCC GCTTCTTCAA GGACAGATGG
    AACCAGTTGG ATCTGGCCAT TGTTTTGTTG TCTATCATGG GCATAACACT GGAGGAAATC
    GAGGTCAACG CTTCCCTTCC CATCAATCCT ACCATCATTC GCATCATGAG GGTGTTGCGC
    ATCACTCGAG TTCTAAAGCT ACTGAAGATG GCGGTGGGAA TGCGAGCTCT ACTAGACACT
    GTGATGCAGG CCCTTCCTCA GGTGGGGAAT CTGGGTCTTC TCTTCATGTT GCTTTTCTTC
    ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT GGTGACCTGA TATGTGATGA GCTACACCCC
    TGTGAAGGTC TTGGCCGTTA TGCCACGTTC AAAAATTTTG GCATGGCTTT CCTCCTGCTG
    TTCCGTGTAT CCACCGGGGA CAACTGGAAT GGCATCATGA AGGATACGCT CAGAGACTGC
    TCCCAAGACA CCGGTATATG CTACAACACG GTGGTGTCCC CCATTTACTT TGTGTCCTTT
    GTGCTGACAG CTCAGTTTGT GCTGGTCAAT GTGGTGATCG CTGTGCTCAT GAAGCACCTA
    GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG
    GAGATGGAAG CTTTGGGTAG AGATAGAGAA ATGCAGAGAG GGCACATGCC ACGTTTGGGT
    TCTGGCGATT TGGGTGGGCC AGGAGGGTCA CCTTGGATAT CAAGAGACCA TCGAGACCTG
    TCAGGACCAC TTTACCCCAT AGACAGCCCA ACAGCAGACA TACGCAGAGA TTTTGAAGAT
    CATTTAAGGA CAGATCCTGA TCCACCTCCG AATCTGGAGC CACCATTAGA GCGGAGGCCT
    ATGTTTGACT CAGTTTCCCT GGTGATCCAG GGCTCGCTGG AAGGAGAGCT CAGCCTCATG
    GACAACCTGT CTGGCTCCAT CTGCCACTAC TACGCTCTGC CCCCCCTTCC CAACAAACAC
    TGCACTGAGA AACAGATTCC TCTAGCCGAG ATGGAGGCTC TATCTCTGGC ATCGGAGAAA
    TCCTGGTCCT TAGCTCTGAC GGATGACTCT GTCCCAGATG ATTTTAACCC CCTCTTACTA
    ACCAGTCAGG AGTGCAATAC CACAGATCCT TCAGATCCAC CAGACACCCG GCAGGCGGAT
    GAACCGACGG ATGAACACTT GCTATCCGTG AAGAAGACCT CAGTGGGCCG CACACATTCT
    CTACCTAATG ACAGCTACAT GTTCCTGCCG CTGCAGTCCA ACTCAAACCA CAGCACCCAC
    ACAACCTCAC CTCACCTCGC TCAGACAGGG TCTACAGCTT CTGTTCAGTC TCAAACAGAA
    GAAGCCAGCC AGCACTTGAC GGTGCCCAGC GATCTGTTCA GACCCATCAG TCCTCACAGT
    TTATCAGACT CAGAGAGCAT CCCTCGAATT CCCCCACCTC GGCGGGCACA CACTCTCTCA
    CGCACACTCC GCAGACAGGT GGCAGTGAGT GCTGACTCTC AGGAGACGCT TTACACAGAG
    GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC AGGGAACTGT CGGACCCCCT TCCACCTCAG
    CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG GTCCCTGCCA CACCAGGCGC CTCCCCAAAA
    CCTTCACGAC CTAGCGTCCA CACGCAGCAC AACCCCTACG ACCAGTACAA TGTGTTCTCC
    AGGAGTTCCC ACTCTCCACC TGTACCACCA CCACCACCAG ACTATAAGAA GCAGGAGGAT
    GTTGACTCGG TGGATCAGGA GGTATCTCGA ATTATCCGAG CGGGACTCAC AGGGAGAAGC
    GATGATGTCA ACCGAGAAGG GACAGGAGAT CAATGTGCAA AACGTCAGCT TAAGAAATAC
    CATAGCGTGG ACACTCAAGG CCAGCGAGCT CTGCTGTTAC CGCGGCCCTT GTCATGGCTT
    GATGACCCAC GCCGGCACTC CATCGAAGTA TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC
    AGTTCCATAT CGTCAGGGTT TGTATCACGA GCCGACAGCC TCCAGCAACA ATCTTCGCCC
    CGTGGACGCA AGAAGAAGAT GAGTCCACCG TGCATTTCTG TAGATCCACC CGAGGATCCG
    GAACTTCCGG GAGGATTCCA TCCTGCTCTG GGGATTGTGC CACCTCCATT GCCAAGCCGG
    GACACTTGCC TGAGGAGGCG CGCTCCCTCC AGTGAATCCA AGGACTCGTT TGATTTGGGA
    GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT CCAAACTCCA AGCTATTGAC TCTGCCTAGC
    TTCTCTTTCG AGAAAACAAG CTCTGAGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214024.1
    CDS12..4664
    Translation

    Target ORF information:

    RefSeq Version XM_017358535.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT
    GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG
    CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA
    GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA
    GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC
    AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC
    TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT
    GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA
    GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG
    GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC
    CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG
    CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT
    TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG
    GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC
    TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC
    ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC
    GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC
    AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC
    TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG
    GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC
    CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC
    AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA
    AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT
    TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA
    GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA
    CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC
    GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG
    CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC
    GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA
    CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAATAT AATGCTGACC
    GGTGTCAGTT GGTCCAGTCC TGATCACACA CAAGAAGCCC AGAGTAAACC CTTTTACTCC
    GATTACTGCC CAACACGACG TCTCATCTAC AACATGTGCA AGAGCCAGTA TCTGGACCTG
    TTCATCACCA TCGTCATCGC CCTCAATGTC ATCACCATGT CTATGGAGCA CTACCACCAA
    CCAAAGGTTT TGAGCGATTC CCTAAAGATC TGTAACTACA TCTTCACAGT CATATTTGTG
    CTGGAGTCCA TCTTCAAACT TGTTGCTTTT GGATTTCGCC GCTTCTTCAA GGACAGATGG
    AACCAGTTGG ATCTGGCCAT TGTTTTGTTG TCTATCATGG GCATAACACT GGAGGAAATC
    GAGGTCAACG CTTCCCTTCC CATCAATCCT ACCATCATTC GCATCATGAG GGTGTTGCGC
    ATCACTCGAG TTCTAAAGCT ACTGAAGATG GCGGTGGGAA TGCGAGCTCT ACTAGACACT
    GTGATGCAGG CCCTTCCTCA GGTGGGGAAT CTGGGTCTTC TCTTCATGTT GCTTTTCTTC
    ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT GGTGACCTGA TATGTGATGA GCTACACCCC
    TGTGAAGGTC TTGGCCGTTA TGCCACGTTC AAAAATTTTG GCATGGCTTT CCTCCTGCTG
    TTCCGTGTAT CCACCGGGGA CAACTGGAAT GGCATCATGA AGGATACGCT CAGAGACTGC
    TCCCAAGACA CCGGTATATG CTACAACACG GTGGTGTCCC CCATTTACTT TGTGTCCTTT
    GTGCTGACAG CTCAGTTTGT GCTGGTCAAT GTGGTGATCG CTGTGCTCAT GAAGCACCTA
    GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG
    GAGATGGAAG CTTTGGGTAG AGATAGAGAA ATGCAGAGAG GGCACATGCC ACGTTTGGGT
    TCTGGCGATT TGGGTGGGCC AGGAGGGTCA CCTTGGATAT CAAGAGACCA TCGAGACCTG
    TCAGGACCAC TTTACCCCAT AGACAGCCCA ACAGCAGACA TACGCAGAGA TTTTGAAGAT
    CATTTAAGGA CAGATCCTGA TCCACCTCCG AATCTGGAGC CACCATTAGA GCGGAGGCCT
    ATGTTTGACT CAGTTTCCCT GGTGATCCAG GGCTCGCTGG AAGGAGAGCT CAGCCTCATG
    GACAACCTGT CTGGCTCCAT CTGCCACTAC TACGCTCTGC CCCCCCTTCC CAACAAACAC
    TGCACTGAGA AACAGATTCC TCTAGCCGAG ATGGAGGCTC TATCTCTGGC ATCGGAGAAA
    TCCTGGTCCT TAGCTCTGAC GGATGACTCT GTCCCAGATG ATTTTAACCC CCTCTTACTA
    ACCAGTCAGG AGTGCAATAC CACAGATCCT TCAGATCCAC CAGACACCCG GCAGGCGGAT
    GAACCGACGG ATGAACACTT GCTATCCGTG AAGAAGACCT CAGTGGGCCG CACACATTCT
    CTACCTAATG ACAGCTACAT GTTCCTGCCG CTGCAGTCCA ACTCAAACCA CAGCACCCAC
    ACAACCTCAC CTCACCTCGC TCAGACAGGG TCTACAGCTT CTGTTCAGTC TCAAACAGAA
    GAAGCCAGCC AGCACTTGAC GGTGCCCAGC GATCTGTTCA GACCCATCAG TCCTCACAGT
    TTATCAGACT CAGAGAGCAT CCCTCGAATT CCCCCACCTC GGCGGGCACA CACTCTCTCA
    CGCACACTCC GCAGACAGGT GGCAGTGAGT GCTGACTCTC AGGAGACGCT TTACACAGAG
    GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC AGGGAACTGT CGGACCCCCT TCCACCTCAG
    CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG GTCCCTGCCA CACCAGGCGC CTCCCCAAAA
    CCTTCACGAC CTAGCGTCCA CACGCAGCAC AACCCCTACG ACCAGTACAA TGTGTTCTCC
    AGGAGTTCCC ACTCTCCACC TGTACCACCA CCACCACCAG ACTATAAGAA GCAGGAGGAT
    GTTGACTCGG TGGATCAGGA GGTATCTCGA ATTATCCGAG CGGGACTCAC AGGGAGAAGC
    GATGATGTCA ACCGAGAAGG GACAGGAGAT CAATGTGCAA AACGTCAGCT TAAGAAATAC
    CATAGCGTGG ACACTCAAGG CCAGCGAGCT CTGCTGTTAC CGCGGCCCTT GTCATGGCTT
    GATGACCCAC GCCGGCACTC CATCGAAGTA TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC
    AGTTCCATAT CGTCAGGGTT TGTATCACGA GCCGACAGCC TCCAGCAACA ATCTTCGCCC
    CGTGGACGCA AGAAGAAGAT GAGTCCACCG TGCATTTCTG TAGATCCACC CGAGGATCCG
    GAACTTCCGG GAGGATTCCA TCCTGCTCTG GGGATTGTGC CACCTCCATT GCCAAGCCGG
    GACACTTGCC TGAGGAGGCG CGCTCCCTCC AGTGAATCCA AGGACTCGTT TGATTTGGGA
    GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT CCAAACTCCA AGCTATTGAC TCTGCCTAGC
    TTCTCTTTCG AGAAAACAAG CTCTGAGCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54518
    Clone ID Related Accession (Same CDS sequence) XM_017358536.1
    Accession Version XM_017358536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT
    GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG
    CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA
    GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA
    GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC
    AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC
    TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT
    GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA
    GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG
    GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC
    CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG
    CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT
    TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG
    GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC
    TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC
    ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC
    GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC
    AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC
    TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG
    GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC
    CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC
    AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA
    AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT
    TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA
    GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA
    CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC
    GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG
    CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC
    GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA
    CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAGTAA GGAGAAGGAG
    CTAGCTGAAG CCCAGAGTAA ACCCTTTTAC TCCGATTACT GCCCAACACG ACGTCTCATC
    TACAACATGT GCAAGAGCCA GTATCTGGAC CTGTTCATCA CCATCGTCAT CGCCCTCAAT
    GTCATCACCA TGTCTATGGA GCACTACCAC CAACCAAAGG TTTTGAGCGA TTCCCTAAAG
    ATCTGTAACT ACATCTTCAC AGTCATATTT GTGCTGGAGT CCATCTTCAA ACTTGTTGCT
    TTTGGATTTC GCCGCTTCTT CAAGGACAGA TGGAACCAGT TGGATCTGGC CATTGTTTTG
    TTGTCTATCA TGGGCATAAC ACTGGAGGAA ATCGAGGTCA ACGCTTCCCT TCCCATCAAT
    CCTACCATCA TTCGCATCAT GAGGGTGTTG CGCATCACTC GAGTTCTAAA GCTACTGAAG
    ATGGCGGTGG GAATGCGAGC TCTACTAGAC ACTGTGATGC AGGCCCTTCC TCAGGTGGGG
    AATCTGGGTC TTCTCTTCAT GTTGCTTTTC TTCATCTTTG CTGCTCTGGG AGTAGAGCTG
    TTTGGTGACC TGATATGTGA TGAGCTACAC CCCTGTGAAG GTCTTGGCCG TTATGCCACG
    TTCAAAAATT TTGGCATGGC TTTCCTCCTG CTGTTCCGTG TATCCACCGG GGACAACTGG
    AATGGCATCA TGAAGGATAC GCTCAGAGAC TGCTCCCAAG ACACCGGTAT ATGCTACAAC
    ACGGTGGTGT CCCCCATTTA CTTTGTGTCC TTTGTGCTGA CAGCTCAGTT TGTGCTGGTC
    AATGTGGTGA TCGCTGTGCT CATGAAGCAC CTAGAGGAAA GCAACAAAGA GGCCAAGGAG
    GCTGAAGCAG AGGCCGAGCT GGAGCTGGAG CAGGAGATGG AAGCTTTGGG TAGAGATAGA
    GAAATGCAGA GAGGGCACAT GCCACGTTTG GGTTCTGGCG ATTTGGGTGG GCCAGGAGGG
    TCACCTTGGA TATCAAGAGA CCATCGAGAC CTGTCAGGAC CACTTTACCC CATAGACAGC
    CCAACAGCAG ACATACGCAG AGATTTTGAA GATCATTTAA GGACAGATCC TGATCCACCT
    CCGAATCTGG AGCCACCATT AGAGCGGAGG CCTATGTTTG ACTCAGTTTC CCTGGTGATC
    CAGGGCTCGC TGGAAGGAGA GCTCAGCCTC ATGGACAACC TGTCTGGCTC CATCTGCCAC
    TACTACGCTC TGCCCCCCCT TCCCAACAAA CACTGCACTG AGAAACAGAT TCCTCTAGCC
    GAGATGGAGG CTCTATCTCT GGCATCGGAG AAATCCTGGT CCTTAGCTCT GACGGATGAC
    TCTGTCCCAG ATGATTTTAA CCCCCTCTTA CTAACCAGTC AGGAGTGCAA TACCACAGAT
    CCTTCAGATC CACCAGACAC CCGGCAGGCG GATGAACCGA CGGATGAACA CTTGCTATCC
    GTGAAGAAGA CCTCAGTGGG CCGCACACAT TCTCTACCTA ATGACAGCTA CATGTTCCTG
    CCGCTGCAGT CCAACTCAAA CCACAGCACC CACACAACCT CACCTCACCT CGCTCAGACA
    GGGTCTACAG CTTCTGTTCA GTCTCAAACA GAAGAAGCCA GCCAGCACTT GACGGTGCCC
    AGCGATCTGT TCAGACCCAT CAGTCCTCAC AGTTTATCAG ACTCAGAGAG CATCCCTCGA
    ATTCCCCCAC CTCGGCGGGC ACACACTCTC TCACGCACAC TCCGCAGACA GGTGGCAGTG
    AGTGCTGACT CTCAGGAGAC GCTTTACACA GAGGGAGGGG AAGCTGAGGG TCCTCTGGGG
    CTCAGGGAAC TGTCGGACCC CCTTCCACCT CAGCAGCAGC AGCACCAGCC TTCCCTCTTT
    CTGGTCCCTG CCACACCAGG CGCCTCCCCA AAACCTTCAC GACCTAGCGT CCACACGCAG
    CACAACCCCT ACGACCAGTA CAATGTGTTC TCCAGGAGTT CCCACTCTCC ACCTGTACCA
    CCACCACCAC CAGACTATAA GAAGCAGGAG GATGTTGACT CGGTGGATCA GGAGGTATCT
    CGAATTATCC GAGCGGGACT CACAGGGAGA AGCGATGATG TCAACCGAGA AGGGACAGGA
    GATCAATGTG CAAAACGTCA GCTTAAGAAA TACCATAGCG TGGACACTCA AGGCCAGCGA
    GCTCTGCTGT TACCGCGGCC CTTGTCATGG CTTGATGACC CACGCCGGCA CTCCATCGAA
    GTATGCTCGT CTGTAGAAAG CAGTCCGCAG CGCAGTTCCA TATCGTCAGG GTTTGTATCA
    CGAGCCGACA GCCTCCAGCA ACAATCTTCG CCCCGTGGAC GCAAGAAGAA GATGAGTCCA
    CCGTGCATTT CTGTAGATCC ACCCGAGGAT CCGGAACTTC CGGGAGGATT CCATCCTGCT
    CTGGGGATTG TGCCACCTCC ATTGCCAAGC CGGGACACTT GCCTGAGGAG GCGCGCTCCC
    TCCAGTGAAT CCAAGGACTC GTTTGATTTG GGAGGAGGAG AGGCGCTGCC TCAAGAGGGC
    GTTCCAAACT CCAAGCTATT GACTCTGCCT AGCTTCTCTT TCGAGAAAAC AAGCTCTGAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214025.1
    CDS12..4637
    Translation

    Target ORF information:

    RefSeq Version XM_017358536.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT
    GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG
    CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA
    GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA
    GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC
    AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC
    TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT
    GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA
    GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG
    GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC
    CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG
    CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT
    TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG
    GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC
    TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC
    ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC
    GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC
    AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC
    TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG
    GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC
    CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC
    AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA
    AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT
    TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA
    GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA
    CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC
    GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG
    CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC
    GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA
    CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAGTAA GGAGAAGGAG
    CTAGCTGAAG CCCAGAGTAA ACCCTTTTAC TCCGATTACT GCCCAACACG ACGTCTCATC
    TACAACATGT GCAAGAGCCA GTATCTGGAC CTGTTCATCA CCATCGTCAT CGCCCTCAAT
    GTCATCACCA TGTCTATGGA GCACTACCAC CAACCAAAGG TTTTGAGCGA TTCCCTAAAG
    ATCTGTAACT ACATCTTCAC AGTCATATTT GTGCTGGAGT CCATCTTCAA ACTTGTTGCT
    TTTGGATTTC GCCGCTTCTT CAAGGACAGA TGGAACCAGT TGGATCTGGC CATTGTTTTG
    TTGTCTATCA TGGGCATAAC ACTGGAGGAA ATCGAGGTCA ACGCTTCCCT TCCCATCAAT
    CCTACCATCA TTCGCATCAT GAGGGTGTTG CGCATCACTC GAGTTCTAAA GCTACTGAAG
    ATGGCGGTGG GAATGCGAGC TCTACTAGAC ACTGTGATGC AGGCCCTTCC TCAGGTGGGG
    AATCTGGGTC TTCTCTTCAT GTTGCTTTTC TTCATCTTTG CTGCTCTGGG AGTAGAGCTG
    TTTGGTGACC TGATATGTGA TGAGCTACAC CCCTGTGAAG GTCTTGGCCG TTATGCCACG
    TTCAAAAATT TTGGCATGGC TTTCCTCCTG CTGTTCCGTG TATCCACCGG GGACAACTGG
    AATGGCATCA TGAAGGATAC GCTCAGAGAC TGCTCCCAAG ACACCGGTAT ATGCTACAAC
    ACGGTGGTGT CCCCCATTTA CTTTGTGTCC TTTGTGCTGA CAGCTCAGTT TGTGCTGGTC
    AATGTGGTGA TCGCTGTGCT CATGAAGCAC CTAGAGGAAA GCAACAAAGA GGCCAAGGAG
    GCTGAAGCAG AGGCCGAGCT GGAGCTGGAG CAGGAGATGG AAGCTTTGGG TAGAGATAGA
    GAAATGCAGA GAGGGCACAT GCCACGTTTG GGTTCTGGCG ATTTGGGTGG GCCAGGAGGG
    TCACCTTGGA TATCAAGAGA CCATCGAGAC CTGTCAGGAC CACTTTACCC CATAGACAGC
    CCAACAGCAG ACATACGCAG AGATTTTGAA GATCATTTAA GGACAGATCC TGATCCACCT
    CCGAATCTGG AGCCACCATT AGAGCGGAGG CCTATGTTTG ACTCAGTTTC CCTGGTGATC
    CAGGGCTCGC TGGAAGGAGA GCTCAGCCTC ATGGACAACC TGTCTGGCTC CATCTGCCAC
    TACTACGCTC TGCCCCCCCT TCCCAACAAA CACTGCACTG AGAAACAGAT TCCTCTAGCC
    GAGATGGAGG CTCTATCTCT GGCATCGGAG AAATCCTGGT CCTTAGCTCT GACGGATGAC
    TCTGTCCCAG ATGATTTTAA CCCCCTCTTA CTAACCAGTC AGGAGTGCAA TACCACAGAT
    CCTTCAGATC CACCAGACAC CCGGCAGGCG GATGAACCGA CGGATGAACA CTTGCTATCC
    GTGAAGAAGA CCTCAGTGGG CCGCACACAT TCTCTACCTA ATGACAGCTA CATGTTCCTG
    CCGCTGCAGT CCAACTCAAA CCACAGCACC CACACAACCT CACCTCACCT CGCTCAGACA
    GGGTCTACAG CTTCTGTTCA GTCTCAAACA GAAGAAGCCA GCCAGCACTT GACGGTGCCC
    AGCGATCTGT TCAGACCCAT CAGTCCTCAC AGTTTATCAG ACTCAGAGAG CATCCCTCGA
    ATTCCCCCAC CTCGGCGGGC ACACACTCTC TCACGCACAC TCCGCAGACA GGTGGCAGTG
    AGTGCTGACT CTCAGGAGAC GCTTTACACA GAGGGAGGGG AAGCTGAGGG TCCTCTGGGG
    CTCAGGGAAC TGTCGGACCC CCTTCCACCT CAGCAGCAGC AGCACCAGCC TTCCCTCTTT
    CTGGTCCCTG CCACACCAGG CGCCTCCCCA AAACCTTCAC GACCTAGCGT CCACACGCAG
    CACAACCCCT ACGACCAGTA CAATGTGTTC TCCAGGAGTT CCCACTCTCC ACCTGTACCA
    CCACCACCAC CAGACTATAA GAAGCAGGAG GATGTTGACT CGGTGGATCA GGAGGTATCT
    CGAATTATCC GAGCGGGACT CACAGGGAGA AGCGATGATG TCAACCGAGA AGGGACAGGA
    GATCAATGTG CAAAACGTCA GCTTAAGAAA TACCATAGCG TGGACACTCA AGGCCAGCGA
    GCTCTGCTGT TACCGCGGCC CTTGTCATGG CTTGATGACC CACGCCGGCA CTCCATCGAA
    GTATGCTCGT CTGTAGAAAG CAGTCCGCAG CGCAGTTCCA TATCGTCAGG GTTTGTATCA
    CGAGCCGACA GCCTCCAGCA ACAATCTTCG CCCCGTGGAC GCAAGAAGAA GATGAGTCCA
    CCGTGCATTT CTGTAGATCC ACCCGAGGAT CCGGAACTTC CGGGAGGATT CCATCCTGCT
    CTGGGGATTG TGCCACCTCC ATTGCCAAGC CGGGACACTT GCCTGAGGAG GCGCGCTCCC
    TCCAGTGAAT CCAAGGACTC GTTTGATTTG GGAGGAGGAG AGGCGCTGCC TCAAGAGGGC
    GTTCCAAACT CCAAGCTATT GACTCTGCCT AGCTTCTCTT TCGAGAAAAC AAGCTCTGAG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148360
    Clone ID Related Accession (Same CDS sequence) XM_021480344.1
    Accession Version XM_021480344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT
    CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG
    GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC
    ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG
    CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG
    TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT
    CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG
    GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC
    CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG
    GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT
    CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC
    CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC
    ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT
    GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG
    GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG
    ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC
    CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC
    ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT
    GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG
    AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT
    CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC
    GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA
    GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC
    TCTGAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336019.1
    CDS785..8296
    Translation

    Target ORF information:

    RefSeq Version XM_021480344.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT
    CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG
    GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC
    ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG
    CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG
    TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT
    CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG
    GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC
    CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG
    GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT
    CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC
    CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC
    ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT
    GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG
    GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG
    ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC
    CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC
    ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT
    GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG
    AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT
    CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC
    GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA
    GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC
    TCTGAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148361
    Clone ID Related Accession (Same CDS sequence) XM_021480345.1
    Accession Version XM_021480345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA ATATAATGCT GACCGGTGTC AGTTGGTCCA GTCCTGATCA CACACAAGAA
    GCCCAGAGTA AACCCTTTTA CTCCGATTAC TGCCCAACAC GACGTCTCAT CTACAACATG
    TGCAAGAGCC AGTATCTGGA CCTGTTCATC ACCATCGTCA TCGCCCTCAA TGTCATCACC
    ATGTCTATGG AGCACTACCA CCAACCAAAG GTTTTGAGCG ATTCCCTAAA GATCTGTAAC
    TACATCTTCA CAGTCATATT TGTGCTGGAG TCCATCTTCA AACTTGTTGC TTTTGGATTT
    CGCCGCTTCT TCAAGGACAG ATGGAACCAG TTGGATCTGG CCATTGTTTT GTTGTCTATC
    ATGGGCATAA CACTGGAGGA AATCGAGGTC AATGCTTCCC TTCCCATCAA CCCTACAATC
    ATTCGCATCA TGAGGGTGTT GCGCATCACT CGAGTTCTAA AGCTACTGAA GATGGCGGTG
    GGAATGCGAG CTCTACTAGA CACTGTGATG CAGGCCCTTC CTCAGGTGGG GAATCTGGGT
    CTTCTCTTCA TGTTGCTTTT CTTCATCTTT GCTGCTCTGG GAGTAGAGCT GTTTGGTGAC
    CTGATATGTG ATGAGCTACA CCCCTGTGAA GGTCTTGGCC GTTATGCCAC GTTCAAGAAT
    TTTGGCATGG CTTTCCTCCT GCTGTTCCGT GTATCCACCG GGGACAACTG GAATGGCATC
    ATGAAGGATA CGCTCAGAGA CTGCTCCCAA GACACCGGTA TATGCTACAA CACGGTGGTG
    TCCCCCATTT ACTTTGTGTC CTTTGTGCTG ACAGCTCAGT TTGTGCTGGT CAATGTGGTG
    ATCGCTGTGC TCATGAAGCA CCTAGAGGAA AGCAACAAAG AGGCCAAGGA GGCTGAAGCA
    GAGGCCGAGC TGGAGCTGGA GCAGGAGATG GAAGCTTTGG GTAGAGATAG AGAAATGCAG
    AGAGGGCACA TGCCACGTTT GGGTTCTGGC GATTTGGGTG GGCCAGGAGG GTCACCTTGG
    ATATCAAGAG ACCATCGAGA CCTGTCAGGA CCACTTTACC CCATAGACAG CCCAACAGCA
    GACATACGCA GAGATTTTGA AGATCATTTA AGGACAGATC CTGATCCACC TCCGAATCTG
    GAGCCACCAT TAGAGCGGAG GCCTATGTTT GACTCAGTTT CCCTGGTGAT CCAGGGCTCG
    CTGGAAGGAG AGCTCAGCCT CATGGACAAC CTGTCTGGCT CCATCTGCCA CTACTACGCT
    CTGCCCCCCC TTCCCAACAA ACACTGCACT GAGAAACAGA TTCCTCTAGC CGAGATGGAG
    GCTCTATCTC TGGCATCGGA GAAATCCTGG TCCTTAGCTC TGACGGATGA CTCTGTCCCA
    GATGATTTTA ACCCCCTCTT ACTAACCAGT CAGGAGTGCA ATACCACAGA TCCTTCAGAT
    CCACCAGACA CCCGGCAGGC GGATGAACCG ACGGATGAAC ACTTGCTATC CGTGAAGAAG
    ACCTCAGTGG GCCGCACACA TTCTCTACCT AATGACAGCT ACATGTTCCT GCCGCTGCAG
    TCCAACTCAA ACCACAGCAC CCACACAACC TCACCTCACC TCGCTCAGAC AGGGTCTACA
    GCTTCTGTTC AGTCTCAAAC AGAAGAAGCC AGCCAGCACT TGACGGTGCC CAGCGATCTG
    TTCAGACCCA TCAGTCCTCA CAGTTTATCA GACTCAGAGA GCATCCCTCG AATTCCCCCA
    CCTCGGCGGG CACACACTCT CTCACGCACA CTCCGCAGAC AGGTGGCAGT GAGTGCTGAC
    TCTCAGGAGA CGCTTTACAC AGAGGGAGGG GAAGCTGAGG GTCCTCTGGG GCTCAGGGAA
    CTGTCGGACC CCCTTCCACC TCAGCAGCAG CAGCACCAGC CTTCCCTCTT TCTGGTCCCT
    GCCACACCAG GCGCCTCCCC AAAACCTTCA CGACCTAGCG TCCACACGCA GCACAACCCC
    TACGACCAGT ACAATGTGTT CTCCAGGAGT TCCCACTCTC CACCTGTACC ACCACCACCA
    CCAGACTATA AGAAGCAGGA GGATGTTGAC TCGGTGGATC AGGAGGTATC TCGAATTATC
    CGAGCGGGAC TCACAGGGAG AAGCGATGAT GTCAACCGAG AAGGGACAGG AGATCAATGT
    GCAAAACGTC AGCTTAAGAA ATACCATAGC GTGGACACTC AAGGCCAGCG AGCTCTGCTG
    TTACCGCGGC CCTTGTCATG GCTTGATGAC CCACGCCGGC ACTCCATCGA AGTATGCTCG
    TCTGTAGAAA GCAGTCCGCA GCGCAGTTCC ATATCGTCAG GGTTTGTATC ACGAGCCGAC
    AGCCTCCAGC AACAATCTTC GCCCCGTGGA CGCAAGAAGA AGATGAGTCC ACCGTGCATT
    TCTGTAGATC CACCCGAGGA TCCGGAACTT CCGGGAGGAT TCCATCCTGC TCTGGGGATT
    GTGCCACCTC CATTGCCAAG CCGGGACACT TGCCTGAGGA GGCGCGCTCC CTCCAGTGAA
    TCCAAGGACT CGTTTGATTT GGGAGGAGGA GAGGCGCTGC CTCAAGAGGG CGTTCCAAAC
    TCCAAGCTAT TGACTCTGCC TAGCTTCTCT TTCGAGAAAA CAAGCTCTGA GCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336020.1
    CDS784..8220
    Translation

    Target ORF information:

    RefSeq Version XM_021480345.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA ATATAATGCT GACCGGTGTC AGTTGGTCCA GTCCTGATCA CACACAAGAA
    GCCCAGAGTA AACCCTTTTA CTCCGATTAC TGCCCAACAC GACGTCTCAT CTACAACATG
    TGCAAGAGCC AGTATCTGGA CCTGTTCATC ACCATCGTCA TCGCCCTCAA TGTCATCACC
    ATGTCTATGG AGCACTACCA CCAACCAAAG GTTTTGAGCG ATTCCCTAAA GATCTGTAAC
    TACATCTTCA CAGTCATATT TGTGCTGGAG TCCATCTTCA AACTTGTTGC TTTTGGATTT
    CGCCGCTTCT TCAAGGACAG ATGGAACCAG TTGGATCTGG CCATTGTTTT GTTGTCTATC
    ATGGGCATAA CACTGGAGGA AATCGAGGTC AATGCTTCCC TTCCCATCAA CCCTACAATC
    ATTCGCATCA TGAGGGTGTT GCGCATCACT CGAGTTCTAA AGCTACTGAA GATGGCGGTG
    GGAATGCGAG CTCTACTAGA CACTGTGATG CAGGCCCTTC CTCAGGTGGG GAATCTGGGT
    CTTCTCTTCA TGTTGCTTTT CTTCATCTTT GCTGCTCTGG GAGTAGAGCT GTTTGGTGAC
    CTGATATGTG ATGAGCTACA CCCCTGTGAA GGTCTTGGCC GTTATGCCAC GTTCAAGAAT
    TTTGGCATGG CTTTCCTCCT GCTGTTCCGT GTATCCACCG GGGACAACTG GAATGGCATC
    ATGAAGGATA CGCTCAGAGA CTGCTCCCAA GACACCGGTA TATGCTACAA CACGGTGGTG
    TCCCCCATTT ACTTTGTGTC CTTTGTGCTG ACAGCTCAGT TTGTGCTGGT CAATGTGGTG
    ATCGCTGTGC TCATGAAGCA CCTAGAGGAA AGCAACAAAG AGGCCAAGGA GGCTGAAGCA
    GAGGCCGAGC TGGAGCTGGA GCAGGAGATG GAAGCTTTGG GTAGAGATAG AGAAATGCAG
    AGAGGGCACA TGCCACGTTT GGGTTCTGGC GATTTGGGTG GGCCAGGAGG GTCACCTTGG
    ATATCAAGAG ACCATCGAGA CCTGTCAGGA CCACTTTACC CCATAGACAG CCCAACAGCA
    GACATACGCA GAGATTTTGA AGATCATTTA AGGACAGATC CTGATCCACC TCCGAATCTG
    GAGCCACCAT TAGAGCGGAG GCCTATGTTT GACTCAGTTT CCCTGGTGAT CCAGGGCTCG
    CTGGAAGGAG AGCTCAGCCT CATGGACAAC CTGTCTGGCT CCATCTGCCA CTACTACGCT
    CTGCCCCCCC TTCCCAACAA ACACTGCACT GAGAAACAGA TTCCTCTAGC CGAGATGGAG
    GCTCTATCTC TGGCATCGGA GAAATCCTGG TCCTTAGCTC TGACGGATGA CTCTGTCCCA
    GATGATTTTA ACCCCCTCTT ACTAACCAGT CAGGAGTGCA ATACCACAGA TCCTTCAGAT
    CCACCAGACA CCCGGCAGGC GGATGAACCG ACGGATGAAC ACTTGCTATC CGTGAAGAAG
    ACCTCAGTGG GCCGCACACA TTCTCTACCT AATGACAGCT ACATGTTCCT GCCGCTGCAG
    TCCAACTCAA ACCACAGCAC CCACACAACC TCACCTCACC TCGCTCAGAC AGGGTCTACA
    GCTTCTGTTC AGTCTCAAAC AGAAGAAGCC AGCCAGCACT TGACGGTGCC CAGCGATCTG
    TTCAGACCCA TCAGTCCTCA CAGTTTATCA GACTCAGAGA GCATCCCTCG AATTCCCCCA
    CCTCGGCGGG CACACACTCT CTCACGCACA CTCCGCAGAC AGGTGGCAGT GAGTGCTGAC
    TCTCAGGAGA CGCTTTACAC AGAGGGAGGG GAAGCTGAGG GTCCTCTGGG GCTCAGGGAA
    CTGTCGGACC CCCTTCCACC TCAGCAGCAG CAGCACCAGC CTTCCCTCTT TCTGGTCCCT
    GCCACACCAG GCGCCTCCCC AAAACCTTCA CGACCTAGCG TCCACACGCA GCACAACCCC
    TACGACCAGT ACAATGTGTT CTCCAGGAGT TCCCACTCTC CACCTGTACC ACCACCACCA
    CCAGACTATA AGAAGCAGGA GGATGTTGAC TCGGTGGATC AGGAGGTATC TCGAATTATC
    CGAGCGGGAC TCACAGGGAG AAGCGATGAT GTCAACCGAG AAGGGACAGG AGATCAATGT
    GCAAAACGTC AGCTTAAGAA ATACCATAGC GTGGACACTC AAGGCCAGCG AGCTCTGCTG
    TTACCGCGGC CCTTGTCATG GCTTGATGAC CCACGCCGGC ACTCCATCGA AGTATGCTCG
    TCTGTAGAAA GCAGTCCGCA GCGCAGTTCC ATATCGTCAG GGTTTGTATC ACGAGCCGAC
    AGCCTCCAGC AACAATCTTC GCCCCGTGGA CGCAAGAAGA AGATGAGTCC ACCGTGCATT
    TCTGTAGATC CACCCGAGGA TCCGGAACTT CCGGGAGGAT TCCATCCTGC TCTGGGGATT
    GTGCCACCTC CATTGCCAAG CCGGGACACT TGCCTGAGGA GGCGCGCTCC CTCCAGTGAA
    TCCAAGGACT CGTTTGATTT GGGAGGAGGA GAGGCGCTGC CTCAAGAGGG CGTTCCAAAC
    TCCAAGCTAT TGACTCTGCC TAGCTTCTCT TTCGAGAAAA CAAGCTCTGA GCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148362
    Clone ID Related Accession (Same CDS sequence) XM_017358537.2
    Accession Version XM_017358537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA GTAAGGAGAA GGAGCTAGCT GAAGCCCAGA GTAAACCCTT TTACTCCGAT
    TACTGCCCAA CACGACGTCT CATCTACAAC ATGTGCAAGA GCCAGTATCT GGACCTGTTC
    ATCACCATCG TCATCGCCCT CAATGTCATC ACCATGTCTA TGGAGCACTA CCACCAACCA
    AAGGTTTTGA GCGATTCCCT AAAGATCTGT AACTACATCT TCACAGTCAT ATTTGTGCTG
    GAGTCCATCT TCAAACTTGT TGCTTTTGGA TTTCGCCGCT TCTTCAAGGA CAGATGGAAC
    CAGTTGGATC TGGCCATTGT TTTGTTGTCT ATCATGGGCA TAACACTGGA GGAAATCGAG
    GTCAATGCTT CCCTTCCCAT CAACCCTACA ATCATTCGCA TCATGAGGGT GTTGCGCATC
    ACTCGAGTTC TAAAGCTACT GAAGATGGCG GTGGGAATGC GAGCTCTACT AGACACTGTG
    ATGCAGGCCC TTCCTCAGGT GGGGAATCTG GGTCTTCTCT TCATGTTGCT TTTCTTCATC
    TTTGCTGCTC TGGGAGTAGA GCTGTTTGGT GACCTGATAT GTGATGAGCT ACACCCCTGT
    GAAGGTCTTG GCCGTTATGC CACGTTCAAG AATTTTGGCA TGGCTTTCCT CCTGCTGTTC
    CGTGTATCCA CCGGGGACAA CTGGAATGGC ATCATGAAGG ATACGCTCAG AGACTGCTCC
    CAAGACACCG GTATATGCTA CAACACGGTG GTGTCCCCCA TTTACTTTGT GTCCTTTGTG
    CTGACAGCTC AGTTTGTGCT GGTCAATGTG GTGATCGCTG TGCTCATGAA GCACCTAGAG
    GAAAGCAACA AAGAGGCCAA GGAGGCTGAA GCAGAGGCCG AGCTGGAGCT GGAGCAGGAG
    ATGGAAGCTT TGGGTAGAGA TAGAGAAATG CAGAGAGGGC ACATGCCACG TTTGGGTTCT
    GGCGATTTGG GTGGGCCAGG AGGGTCACCT TGGATATCAA GAGACCATCG AGACCTGTCA
    GGACCACTTT ACCCCATAGA CAGCCCAACA GCAGACATAC GCAGAGATTT TGAAGATCAT
    TTAAGGACAG ATCCTGATCC ACCTCCGAAT CTGGAGCCAC CATTAGAGCG GAGGCCTATG
    TTTGACTCAG TTTCCCTGGT GATCCAGGGC TCGCTGGAAG GAGAGCTCAG CCTCATGGAC
    AACCTGTCTG GCTCCATCTG CCACTACTAC GCTCTGCCCC CCCTTCCCAA CAAACACTGC
    ACTGAGAAAC AGATTCCTCT AGCCGAGATG GAGGCTCTAT CTCTGGCATC GGAGAAATCC
    TGGTCCTTAG CTCTGACGGA TGACTCTGTC CCAGATGATT TTAACCCCCT CTTACTAACC
    AGTCAGGAGT GCAATACCAC AGATCCTTCA GATCCACCAG ACACCCGGCA GGCGGATGAA
    CCGACGGATG AACACTTGCT ATCCGTGAAG AAGACCTCAG TGGGCCGCAC ACATTCTCTA
    CCTAATGACA GCTACATGTT CCTGCCGCTG CAGTCCAACT CAAACCACAG CACCCACACA
    ACCTCACCTC ACCTCGCTCA GACAGGGTCT ACAGCTTCTG TTCAGTCTCA AACAGAAGAA
    GCCAGCCAGC ACTTGACGGT GCCCAGCGAT CTGTTCAGAC CCATCAGTCC TCACAGTTTA
    TCAGACTCAG AGAGCATCCC TCGAATTCCC CCACCTCGGC GGGCACACAC TCTCTCACGC
    ACACTCCGCA GACAGGTGGC AGTGAGTGCT GACTCTCAGG AGACGCTTTA CACAGAGGGA
    GGGGAAGCTG AGGGTCCTCT GGGGCTCAGG GAACTGTCGG ACCCCCTTCC ACCTCAGCAG
    CAGCAGCACC AGCCTTCCCT CTTTCTGGTC CCTGCCACAC CAGGCGCCTC CCCAAAACCT
    TCACGACCTA GCGTCCACAC GCAGCACAAC CCCTACGACC AGTACAATGT GTTCTCCAGG
    AGTTCCCACT CTCCACCTGT ACCACCACCA CCACCAGACT ATAAGAAGCA GGAGGATGTT
    GACTCGGTGG ATCAGGAGGT ATCTCGAATT ATCCGAGCGG GACTCACAGG GAGAAGCGAT
    GATGTCAACC GAGAAGGGAC AGGAGATCAA TGTGCAAAAC GTCAGCTTAA GAAATACCAT
    AGCGTGGACA CTCAAGGCCA GCGAGCTCTG CTGTTACCGC GGCCCTTGTC ATGGCTTGAT
    GACCCACGCC GGCACTCCAT CGAAGTATGC TCGTCTGTAG AAAGCAGTCC GCAGCGCAGT
    TCCATATCGT CAGGGTTTGT ATCACGAGCC GACAGCCTCC AGCAACAATC TTCGCCCCGT
    GGACGCAAGA AGAAGATGAG TCCACCGTGC ATTTCTGTAG ATCCACCCGA GGATCCGGAA
    CTTCCGGGAG GATTCCATCC TGCTCTGGGG ATTGTGCCAC CTCCATTGCC AAGCCGGGAC
    ACTTGCCTGA GGAGGCGCGC TCCCTCCAGT GAATCCAAGG ACTCGTTTGA TTTGGGAGGA
    GGAGAGGCGC TGCCTCAAGA GGGCGTTCCA AACTCCAAGC TATTGACTCT GCCTAGCTTC
    TCTTTCGAGA AAACAAGCTC TGAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214026.2
    CDS784..8193
    Translation

    Target ORF information:

    RefSeq Version XM_017358537.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA GTAAGGAGAA GGAGCTAGCT GAAGCCCAGA GTAAACCCTT TTACTCCGAT
    TACTGCCCAA CACGACGTCT CATCTACAAC ATGTGCAAGA GCCAGTATCT GGACCTGTTC
    ATCACCATCG TCATCGCCCT CAATGTCATC ACCATGTCTA TGGAGCACTA CCACCAACCA
    AAGGTTTTGA GCGATTCCCT AAAGATCTGT AACTACATCT TCACAGTCAT ATTTGTGCTG
    GAGTCCATCT TCAAACTTGT TGCTTTTGGA TTTCGCCGCT TCTTCAAGGA CAGATGGAAC
    CAGTTGGATC TGGCCATTGT TTTGTTGTCT ATCATGGGCA TAACACTGGA GGAAATCGAG
    GTCAATGCTT CCCTTCCCAT CAACCCTACA ATCATTCGCA TCATGAGGGT GTTGCGCATC
    ACTCGAGTTC TAAAGCTACT GAAGATGGCG GTGGGAATGC GAGCTCTACT AGACACTGTG
    ATGCAGGCCC TTCCTCAGGT GGGGAATCTG GGTCTTCTCT TCATGTTGCT TTTCTTCATC
    TTTGCTGCTC TGGGAGTAGA GCTGTTTGGT GACCTGATAT GTGATGAGCT ACACCCCTGT
    GAAGGTCTTG GCCGTTATGC CACGTTCAAG AATTTTGGCA TGGCTTTCCT CCTGCTGTTC
    CGTGTATCCA CCGGGGACAA CTGGAATGGC ATCATGAAGG ATACGCTCAG AGACTGCTCC
    CAAGACACCG GTATATGCTA CAACACGGTG GTGTCCCCCA TTTACTTTGT GTCCTTTGTG
    CTGACAGCTC AGTTTGTGCT GGTCAATGTG GTGATCGCTG TGCTCATGAA GCACCTAGAG
    GAAAGCAACA AAGAGGCCAA GGAGGCTGAA GCAGAGGCCG AGCTGGAGCT GGAGCAGGAG
    ATGGAAGCTT TGGGTAGAGA TAGAGAAATG CAGAGAGGGC ACATGCCACG TTTGGGTTCT
    GGCGATTTGG GTGGGCCAGG AGGGTCACCT TGGATATCAA GAGACCATCG AGACCTGTCA
    GGACCACTTT ACCCCATAGA CAGCCCAACA GCAGACATAC GCAGAGATTT TGAAGATCAT
    TTAAGGACAG ATCCTGATCC ACCTCCGAAT CTGGAGCCAC CATTAGAGCG GAGGCCTATG
    TTTGACTCAG TTTCCCTGGT GATCCAGGGC TCGCTGGAAG GAGAGCTCAG CCTCATGGAC
    AACCTGTCTG GCTCCATCTG CCACTACTAC GCTCTGCCCC CCCTTCCCAA CAAACACTGC
    ACTGAGAAAC AGATTCCTCT AGCCGAGATG GAGGCTCTAT CTCTGGCATC GGAGAAATCC
    TGGTCCTTAG CTCTGACGGA TGACTCTGTC CCAGATGATT TTAACCCCCT CTTACTAACC
    AGTCAGGAGT GCAATACCAC AGATCCTTCA GATCCACCAG ACACCCGGCA GGCGGATGAA
    CCGACGGATG AACACTTGCT ATCCGTGAAG AAGACCTCAG TGGGCCGCAC ACATTCTCTA
    CCTAATGACA GCTACATGTT CCTGCCGCTG CAGTCCAACT CAAACCACAG CACCCACACA
    ACCTCACCTC ACCTCGCTCA GACAGGGTCT ACAGCTTCTG TTCAGTCTCA AACAGAAGAA
    GCCAGCCAGC ACTTGACGGT GCCCAGCGAT CTGTTCAGAC CCATCAGTCC TCACAGTTTA
    TCAGACTCAG AGAGCATCCC TCGAATTCCC CCACCTCGGC GGGCACACAC TCTCTCACGC
    ACACTCCGCA GACAGGTGGC AGTGAGTGCT GACTCTCAGG AGACGCTTTA CACAGAGGGA
    GGGGAAGCTG AGGGTCCTCT GGGGCTCAGG GAACTGTCGG ACCCCCTTCC ACCTCAGCAG
    CAGCAGCACC AGCCTTCCCT CTTTCTGGTC CCTGCCACAC CAGGCGCCTC CCCAAAACCT
    TCACGACCTA GCGTCCACAC GCAGCACAAC CCCTACGACC AGTACAATGT GTTCTCCAGG
    AGTTCCCACT CTCCACCTGT ACCACCACCA CCACCAGACT ATAAGAAGCA GGAGGATGTT
    GACTCGGTGG ATCAGGAGGT ATCTCGAATT ATCCGAGCGG GACTCACAGG GAGAAGCGAT
    GATGTCAACC GAGAAGGGAC AGGAGATCAA TGTGCAAAAC GTCAGCTTAA GAAATACCAT
    AGCGTGGACA CTCAAGGCCA GCGAGCTCTG CTGTTACCGC GGCCCTTGTC ATGGCTTGAT
    GACCCACGCC GGCACTCCAT CGAAGTATGC TCGTCTGTAG AAAGCAGTCC GCAGCGCAGT
    TCCATATCGT CAGGGTTTGT ATCACGAGCC GACAGCCTCC AGCAACAATC TTCGCCCCGT
    GGACGCAAGA AGAAGATGAG TCCACCGTGC ATTTCTGTAG ATCCACCCGA GGATCCGGAA
    CTTCCGGGAG GATTCCATCC TGCTCTGGGG ATTGTGCCAC CTCCATTGCC AAGCCGGGAC
    ACTTGCCTGA GGAGGCGCGC TCCCTCCAGT GAATCCAAGG ACTCGTTTGA TTTGGGAGGA
    GGAGAGGCGC TGCCTCAAGA GGGCGTTCCA AACTCCAAGC TATTGACTCT GCCTAGCTTC
    TCTTTCGAGA AAACAAGCTC TGAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148363
    Clone ID Related Accession (Same CDS sequence) XM_021480346.1
    Accession Version XM_021480346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA AAGCCCAGAG TAAACCCTTT TACTCCGATT ACTGCCCAAC ACGACGTCTC
    ATCTACAACA TGTGCAAGAG CCAGTATCTG GACCTGTTCA TCACCATCGT CATCGCCCTC
    AATGTCATCA CCATGTCTAT GGAGCACTAC CACCAACCAA AGGTTTTGAG CGATTCCCTA
    AAGATCTGTA ACTACATCTT CACAGTCATA TTTGTGCTGG AGTCCATCTT CAAACTTGTT
    GCTTTTGGAT TTCGCCGCTT CTTCAAGGAC AGATGGAACC AGTTGGATCT GGCCATTGTT
    TTGTTGTCTA TCATGGGCAT AACACTGGAG GAAATCGAGG TCAATGCTTC CCTTCCCATC
    AACCCTACAA TCATTCGCAT CATGAGGGTG TTGCGCATCA CTCGAGTTCT AAAGCTACTG
    AAGATGGCGG TGGGAATGCG AGCTCTACTA GACACTGTGA TGCAGGCCCT TCCTCAGGTG
    GGGAATCTGG GTCTTCTCTT CATGTTGCTT TTCTTCATCT TTGCTGCTCT GGGAGTAGAG
    CTGTTTGGTG ACCTGATATG TGATGAGCTA CACCCCTGTG AAGGTCTTGG CCGTTATGCC
    ACGTTCAAGA ATTTTGGCAT GGCTTTCCTC CTGCTGTTCC GTGTATCCAC CGGGGACAAC
    TGGAATGGCA TCATGAAGGA TACGCTCAGA GACTGCTCCC AAGACACCGG TATATGCTAC
    AACACGGTGG TGTCCCCCAT TTACTTTGTG TCCTTTGTGC TGACAGCTCA GTTTGTGCTG
    GTCAATGTGG TGATCGCTGT GCTCATGAAG CACCTAGAGG AAAGCAACAA AGAGGCCAAG
    GAGGCTGAAG CAGAGGCCGA GCTGGAGCTG GAGCAGGAGA TGGAAGCTTT GGGTAGAGAT
    AGAGAAATGC AGAGAGGGCA CATGCCACGT TTGGGTTCTG GCGATTTGGG TGGGCCAGGA
    GGGTCACCTT GGATATCAAG AGACCATCGA GACCTGTCAG GACCACTTTA CCCCATAGAC
    AGCCCAACAG CAGACATACG CAGAGATTTT GAAGATCATT TAAGGACAGA TCCTGATCCA
    CCTCCGAATC TGGAGCCACC ATTAGAGCGG AGGCCTATGT TTGACTCAGT TTCCCTGGTG
    ATCCAGGGCT CGCTGGAAGG AGAGCTCAGC CTCATGGACA ACCTGTCTGG CTCCATCTGC
    CACTACTACG CTCTGCCCCC CCTTCCCAAC AAACACTGCA CTGAGAAACA GATTCCTCTA
    GCCGAGATGG AGGCTCTATC TCTGGCATCG GAGAAATCCT GGTCCTTAGC TCTGACGGAT
    GACTCTGTCC CAGATGATTT TAACCCCCTC TTACTAACCA GTCAGGAGTG CAATACCACA
    GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA
    TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC
    CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG
    ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG
    CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT
    CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA
    GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG
    GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC
    TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG
    CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA
    CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA
    TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA
    GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG
    CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC
    GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA
    TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT
    CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT
    GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT
    CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG
    GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT
    GAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336021.1
    CDS783..8171
    Translation

    Target ORF information:

    RefSeq Version XM_021480346.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG
    TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA
    GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG
    ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA
    GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT
    AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC
    GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC
    AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC
    AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC
    TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG
    GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC
    TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA
    TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC
    GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC
    GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT
    CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC
    ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT
    GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG
    ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC
    TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG
    ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA
    CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG
    ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT
    TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT
    ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC
    AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA
    GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC
    GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT
    TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA
    AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA
    AAACGAAGGA AAGCCCAGAG TAAACCCTTT TACTCCGATT ACTGCCCAAC ACGACGTCTC
    ATCTACAACA TGTGCAAGAG CCAGTATCTG GACCTGTTCA TCACCATCGT CATCGCCCTC
    AATGTCATCA CCATGTCTAT GGAGCACTAC CACCAACCAA AGGTTTTGAG CGATTCCCTA
    AAGATCTGTA ACTACATCTT CACAGTCATA TTTGTGCTGG AGTCCATCTT CAAACTTGTT
    GCTTTTGGAT TTCGCCGCTT CTTCAAGGAC AGATGGAACC AGTTGGATCT GGCCATTGTT
    TTGTTGTCTA TCATGGGCAT AACACTGGAG GAAATCGAGG TCAATGCTTC CCTTCCCATC
    AACCCTACAA TCATTCGCAT CATGAGGGTG TTGCGCATCA CTCGAGTTCT AAAGCTACTG
    AAGATGGCGG TGGGAATGCG AGCTCTACTA GACACTGTGA TGCAGGCCCT TCCTCAGGTG
    GGGAATCTGG GTCTTCTCTT CATGTTGCTT TTCTTCATCT TTGCTGCTCT GGGAGTAGAG
    CTGTTTGGTG ACCTGATATG TGATGAGCTA CACCCCTGTG AAGGTCTTGG CCGTTATGCC
    ACGTTCAAGA ATTTTGGCAT GGCTTTCCTC CTGCTGTTCC GTGTATCCAC CGGGGACAAC
    TGGAATGGCA TCATGAAGGA TACGCTCAGA GACTGCTCCC AAGACACCGG TATATGCTAC
    AACACGGTGG TGTCCCCCAT TTACTTTGTG TCCTTTGTGC TGACAGCTCA GTTTGTGCTG
    GTCAATGTGG TGATCGCTGT GCTCATGAAG CACCTAGAGG AAAGCAACAA AGAGGCCAAG
    GAGGCTGAAG CAGAGGCCGA GCTGGAGCTG GAGCAGGAGA TGGAAGCTTT GGGTAGAGAT
    AGAGAAATGC AGAGAGGGCA CATGCCACGT TTGGGTTCTG GCGATTTGGG TGGGCCAGGA
    GGGTCACCTT GGATATCAAG AGACCATCGA GACCTGTCAG GACCACTTTA CCCCATAGAC
    AGCCCAACAG CAGACATACG CAGAGATTTT GAAGATCATT TAAGGACAGA TCCTGATCCA
    CCTCCGAATC TGGAGCCACC ATTAGAGCGG AGGCCTATGT TTGACTCAGT TTCCCTGGTG
    ATCCAGGGCT CGCTGGAAGG AGAGCTCAGC CTCATGGACA ACCTGTCTGG CTCCATCTGC
    CACTACTACG CTCTGCCCCC CCTTCCCAAC AAACACTGCA CTGAGAAACA GATTCCTCTA
    GCCGAGATGG AGGCTCTATC TCTGGCATCG GAGAAATCCT GGTCCTTAGC TCTGACGGAT
    GACTCTGTCC CAGATGATTT TAACCCCCTC TTACTAACCA GTCAGGAGTG CAATACCACA
    GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA
    TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC
    CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG
    ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG
    CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT
    CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA
    GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG
    GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC
    TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG
    CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA
    CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA
    TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA
    GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG
    CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC
    GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA
    TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT
    CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT
    GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT
    CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG
    GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT
    GAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148364
    Clone ID Related Accession (Same CDS sequence) XM_021480347.1
    Accession Version XM_021480347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
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    5881
    5941
    6001
    6061
    6121
    6181
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    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGACCACA
    GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA
    TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC
    CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG
    ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG
    CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT
    CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA
    GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG
    GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC
    TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG
    CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA
    CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA
    TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA
    GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG
    CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC
    GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA
    TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT
    CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT
    GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT
    CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG
    GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT
    GAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336022.1
    CDS784..8172
    Translation

    Target ORF information:

    RefSeq Version XM_021480347.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGACCACA
    GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA
    TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC
    CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG
    ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG
    CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT
    CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA
    GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG
    GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC
    TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG
    CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA
    CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA
    TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA
    GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG
    CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC
    GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA
    TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT
    CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT
    GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT
    CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG
    GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT
    GAGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148365
    Clone ID Related Accession (Same CDS sequence) XM_017358535.2
    Accession Version XM_017358535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAATATAA TGCTGACCGG TGTCAGTTGG TCCAGTCCTG ATCACACACA AGAAGCCCAG
    AGTAAACCCT TTTACTCCGA TTACTGCCCA ACACGACGTC TCATCTACAA CATGTGCAAG
    AGCCAGTATC TGGACCTGTT CATCACCATC GTCATCGCCC TCAATGTCAT CACCATGTCT
    ATGGAGCACT ACCACCAACC AAAGGTTTTG AGCGATTCCC TAAAGATCTG TAACTACATC
    TTCACAGTCA TATTTGTGCT GGAGTCCATC TTCAAACTTG TTGCTTTTGG ATTTCGCCGC
    TTCTTCAAGG ACAGATGGAA CCAGTTGGAT CTGGCCATTG TTTTGTTGTC TATCATGGGC
    ATAACACTGG AGGAAATCGA GGTCAATGCT TCCCTTCCCA TCAACCCTAC AATCATTCGC
    ATCATGAGGG TGTTGCGCAT CACTCGAGTT CTAAAGCTAC TGAAGATGGC GGTGGGAATG
    CGAGCTCTAC TAGACACTGT GATGCAGGCC CTTCCTCAGG TGGGGAATCT GGGTCTTCTC
    TTCATGTTGC TTTTCTTCAT CTTTGCTGCT CTGGGAGTAG AGCTGTTTGG TGACCTGATA
    TGTGATGAGC TACACCCCTG TGAAGGTCTT GGCCGTTATG CCACGTTCAA GAATTTTGGC
    ATGGCTTTCC TCCTGCTGTT CCGTGTATCC ACCGGGGACA ACTGGAATGG CATCATGAAG
    GATACGCTCA GAGACTGCTC CCAAGACACC GGTATATGCT ACAACACGGT GGTGTCCCCC
    ATTTACTTTG TGTCCTTTGT GCTGACAGCT CAGTTTGTGC TGGTCAATGT GGTGATCGCT
    GTGCTCATGA AGCACCTAGA GGAAAGCAAC AAAGAGGCCA AGGAGGCTGA AGCAGAGGCC
    GAGCTGGAGC TGGAGCAGGA GATGGAAGCT TTGGGTAGAG ATAGAGAAAT GCAGAGAGGG
    CACATGCCAC GTTTGGGTTC TGGCGATTTG GGTGGGCCAG GAGGGTCACC TTGGATATCA
    AGAGACCATC GAGACCTGTC AGGACCACTT TACCCCATAG ACAGCCCAAC AGCAGACATA
    CGCAGAGATT TTGAAGATCA TTTAAGGACA GATCCTGATC CACCTCCGAA TCTGGAGCCA
    CCATTAGAGC GGAGGCCTAT GTTTGACTCA GTTTCCCTGG TGATCCAGGG CTCGCTGGAA
    GGAGAGCTCA GCCTCATGGA CAACCTGTCT GGCTCCATCT GCCACTACTA CGCTCTGCCC
    CCCCTTCCCA ACAAACACTG CACTGAGAAA CAGATTCCTC TAGCCGAGAT GGAGGCTCTA
    TCTCTGGCAT CGGAGAAATC CTGGTCCTTA GCTCTGACGG ATGACTCTGT CCCAGATGAT
    TTTAACCCCC TCTTACTAAC CAGTCAGGAG TGCAATACCA CAGATCCTTC AGATCCACCA
    GACACCCGGC AGGCGGATGA ACCGACGGAT GAACACTTGC TATCCGTGAA GAAGACCTCA
    GTGGGCCGCA CACATTCTCT ACCTAATGAC AGCTACATGT TCCTGCCGCT GCAGTCCAAC
    TCAAACCACA GCACCCACAC AACCTCACCT CACCTCGCTC AGACAGGGTC TACAGCTTCT
    GTTCAGTCTC AAACAGAAGA AGCCAGCCAG CACTTGACGG TGCCCAGCGA TCTGTTCAGA
    CCCATCAGTC CTCACAGTTT ATCAGACTCA GAGAGCATCC CTCGAATTCC CCCACCTCGG
    CGGGCACACA CTCTCTCACG CACACTCCGC AGACAGGTGG CAGTGAGTGC TGACTCTCAG
    GAGACGCTTT ACACAGAGGG AGGGGAAGCT GAGGGTCCTC TGGGGCTCAG GGAACTGTCG
    GACCCCCTTC CACCTCAGCA GCAGCAGCAC CAGCCTTCCC TCTTTCTGGT CCCTGCCACA
    CCAGGCGCCT CCCCAAAACC TTCACGACCT AGCGTCCACA CGCAGCACAA CCCCTACGAC
    CAGTACAATG TGTTCTCCAG GAGTTCCCAC TCTCCACCTG TACCACCACC ACCACCAGAC
    TATAAGAAGC AGGAGGATGT TGACTCGGTG GATCAGGAGG TATCTCGAAT TATCCGAGCG
    GGACTCACAG GGAGAAGCGA TGATGTCAAC CGAGAAGGGA CAGGAGATCA ATGTGCAAAA
    CGTCAGCTTA AGAAATACCA TAGCGTGGAC ACTCAAGGCC AGCGAGCTCT GCTGTTACCG
    CGGCCCTTGT CATGGCTTGA TGACCCACGC CGGCACTCCA TCGAAGTATG CTCGTCTGTA
    GAAAGCAGTC CGCAGCGCAG TTCCATATCG TCAGGGTTTG TATCACGAGC CGACAGCCTC
    CAGCAACAAT CTTCGCCCCG TGGACGCAAG AAGAAGATGA GTCCACCGTG CATTTCTGTA
    GATCCACCCG AGGATCCGGA ACTTCCGGGA GGATTCCATC CTGCTCTGGG GATTGTGCCA
    CCTCCATTGC CAAGCCGGGA CACTTGCCTG AGGAGGCGCG CTCCCTCCAG TGAATCCAAG
    GACTCGTTTG ATTTGGGAGG AGGAGAGGCG CTGCCTCAAG AGGGCGTTCC AAACTCCAAG
    CTATTGACTC TGCCTAGCTT CTCTTTCGAG AAAACAAGCT CTGAGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214024.2
    CDS783..8153
    Translation

    Target ORF information:

    RefSeq Version XM_017358535.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAATATAA TGCTGACCGG TGTCAGTTGG TCCAGTCCTG ATCACACACA AGAAGCCCAG
    AGTAAACCCT TTTACTCCGA TTACTGCCCA ACACGACGTC TCATCTACAA CATGTGCAAG
    AGCCAGTATC TGGACCTGTT CATCACCATC GTCATCGCCC TCAATGTCAT CACCATGTCT
    ATGGAGCACT ACCACCAACC AAAGGTTTTG AGCGATTCCC TAAAGATCTG TAACTACATC
    TTCACAGTCA TATTTGTGCT GGAGTCCATC TTCAAACTTG TTGCTTTTGG ATTTCGCCGC
    TTCTTCAAGG ACAGATGGAA CCAGTTGGAT CTGGCCATTG TTTTGTTGTC TATCATGGGC
    ATAACACTGG AGGAAATCGA GGTCAATGCT TCCCTTCCCA TCAACCCTAC AATCATTCGC
    ATCATGAGGG TGTTGCGCAT CACTCGAGTT CTAAAGCTAC TGAAGATGGC GGTGGGAATG
    CGAGCTCTAC TAGACACTGT GATGCAGGCC CTTCCTCAGG TGGGGAATCT GGGTCTTCTC
    TTCATGTTGC TTTTCTTCAT CTTTGCTGCT CTGGGAGTAG AGCTGTTTGG TGACCTGATA
    TGTGATGAGC TACACCCCTG TGAAGGTCTT GGCCGTTATG CCACGTTCAA GAATTTTGGC
    ATGGCTTTCC TCCTGCTGTT CCGTGTATCC ACCGGGGACA ACTGGAATGG CATCATGAAG
    GATACGCTCA GAGACTGCTC CCAAGACACC GGTATATGCT ACAACACGGT GGTGTCCCCC
    ATTTACTTTG TGTCCTTTGT GCTGACAGCT CAGTTTGTGC TGGTCAATGT GGTGATCGCT
    GTGCTCATGA AGCACCTAGA GGAAAGCAAC AAAGAGGCCA AGGAGGCTGA AGCAGAGGCC
    GAGCTGGAGC TGGAGCAGGA GATGGAAGCT TTGGGTAGAG ATAGAGAAAT GCAGAGAGGG
    CACATGCCAC GTTTGGGTTC TGGCGATTTG GGTGGGCCAG GAGGGTCACC TTGGATATCA
    AGAGACCATC GAGACCTGTC AGGACCACTT TACCCCATAG ACAGCCCAAC AGCAGACATA
    CGCAGAGATT TTGAAGATCA TTTAAGGACA GATCCTGATC CACCTCCGAA TCTGGAGCCA
    CCATTAGAGC GGAGGCCTAT GTTTGACTCA GTTTCCCTGG TGATCCAGGG CTCGCTGGAA
    GGAGAGCTCA GCCTCATGGA CAACCTGTCT GGCTCCATCT GCCACTACTA CGCTCTGCCC
    CCCCTTCCCA ACAAACACTG CACTGAGAAA CAGATTCCTC TAGCCGAGAT GGAGGCTCTA
    TCTCTGGCAT CGGAGAAATC CTGGTCCTTA GCTCTGACGG ATGACTCTGT CCCAGATGAT
    TTTAACCCCC TCTTACTAAC CAGTCAGGAG TGCAATACCA CAGATCCTTC AGATCCACCA
    GACACCCGGC AGGCGGATGA ACCGACGGAT GAACACTTGC TATCCGTGAA GAAGACCTCA
    GTGGGCCGCA CACATTCTCT ACCTAATGAC AGCTACATGT TCCTGCCGCT GCAGTCCAAC
    TCAAACCACA GCACCCACAC AACCTCACCT CACCTCGCTC AGACAGGGTC TACAGCTTCT
    GTTCAGTCTC AAACAGAAGA AGCCAGCCAG CACTTGACGG TGCCCAGCGA TCTGTTCAGA
    CCCATCAGTC CTCACAGTTT ATCAGACTCA GAGAGCATCC CTCGAATTCC CCCACCTCGG
    CGGGCACACA CTCTCTCACG CACACTCCGC AGACAGGTGG CAGTGAGTGC TGACTCTCAG
    GAGACGCTTT ACACAGAGGG AGGGGAAGCT GAGGGTCCTC TGGGGCTCAG GGAACTGTCG
    GACCCCCTTC CACCTCAGCA GCAGCAGCAC CAGCCTTCCC TCTTTCTGGT CCCTGCCACA
    CCAGGCGCCT CCCCAAAACC TTCACGACCT AGCGTCCACA CGCAGCACAA CCCCTACGAC
    CAGTACAATG TGTTCTCCAG GAGTTCCCAC TCTCCACCTG TACCACCACC ACCACCAGAC
    TATAAGAAGC AGGAGGATGT TGACTCGGTG GATCAGGAGG TATCTCGAAT TATCCGAGCG
    GGACTCACAG GGAGAAGCGA TGATGTCAAC CGAGAAGGGA CAGGAGATCA ATGTGCAAAA
    CGTCAGCTTA AGAAATACCA TAGCGTGGAC ACTCAAGGCC AGCGAGCTCT GCTGTTACCG
    CGGCCCTTGT CATGGCTTGA TGACCCACGC CGGCACTCCA TCGAAGTATG CTCGTCTGTA
    GAAAGCAGTC CGCAGCGCAG TTCCATATCG TCAGGGTTTG TATCACGAGC CGACAGCCTC
    CAGCAACAAT CTTCGCCCCG TGGACGCAAG AAGAAGATGA GTCCACCGTG CATTTCTGTA
    GATCCACCCG AGGATCCGGA ACTTCCGGGA GGATTCCATC CTGCTCTGGG GATTGTGCCA
    CCTCCATTGC CAAGCCGGGA CACTTGCCTG AGGAGGCGCG CTCCCTCCAG TGAATCCAAG
    GACTCGTTTG ATTTGGGAGG AGGAGAGGCG CTGCCTCAAG AGGGCGTTCC AAACTCCAAG
    CTATTGACTC TGCCTAGCTT CTCTTTCGAG AAAACAAGCT CTGAGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148366
    Clone ID Related Accession (Same CDS sequence) XM_017358536.2
    Accession Version XM_017358536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAGTAAGG AGAAGGAGCT AGCTGAAGCC CAGAGTAAAC CCTTTTACTC CGATTACTGC
    CCAACACGAC GTCTCATCTA CAACATGTGC AAGAGCCAGT ATCTGGACCT GTTCATCACC
    ATCGTCATCG CCCTCAATGT CATCACCATG TCTATGGAGC ACTACCACCA ACCAAAGGTT
    TTGAGCGATT CCCTAAAGAT CTGTAACTAC ATCTTCACAG TCATATTTGT GCTGGAGTCC
    ATCTTCAAAC TTGTTGCTTT TGGATTTCGC CGCTTCTTCA AGGACAGATG GAACCAGTTG
    GATCTGGCCA TTGTTTTGTT GTCTATCATG GGCATAACAC TGGAGGAAAT CGAGGTCAAT
    GCTTCCCTTC CCATCAACCC TACAATCATT CGCATCATGA GGGTGTTGCG CATCACTCGA
    GTTCTAAAGC TACTGAAGAT GGCGGTGGGA ATGCGAGCTC TACTAGACAC TGTGATGCAG
    GCCCTTCCTC AGGTGGGGAA TCTGGGTCTT CTCTTCATGT TGCTTTTCTT CATCTTTGCT
    GCTCTGGGAG TAGAGCTGTT TGGTGACCTG ATATGTGATG AGCTACACCC CTGTGAAGGT
    CTTGGCCGTT ATGCCACGTT CAAGAATTTT GGCATGGCTT TCCTCCTGCT GTTCCGTGTA
    TCCACCGGGG ACAACTGGAA TGGCATCATG AAGGATACGC TCAGAGACTG CTCCCAAGAC
    ACCGGTATAT GCTACAACAC GGTGGTGTCC CCCATTTACT TTGTGTCCTT TGTGCTGACA
    GCTCAGTTTG TGCTGGTCAA TGTGGTGATC GCTGTGCTCA TGAAGCACCT AGAGGAAAGC
    AACAAAGAGG CCAAGGAGGC TGAAGCAGAG GCCGAGCTGG AGCTGGAGCA GGAGATGGAA
    GCTTTGGGTA GAGATAGAGA AATGCAGAGA GGGCACATGC CACGTTTGGG TTCTGGCGAT
    TTGGGTGGGC CAGGAGGGTC ACCTTGGATA TCAAGAGACC ATCGAGACCT GTCAGGACCA
    CTTTACCCCA TAGACAGCCC AACAGCAGAC ATACGCAGAG ATTTTGAAGA TCATTTAAGG
    ACAGATCCTG ATCCACCTCC GAATCTGGAG CCACCATTAG AGCGGAGGCC TATGTTTGAC
    TCAGTTTCCC TGGTGATCCA GGGCTCGCTG GAAGGAGAGC TCAGCCTCAT GGACAACCTG
    TCTGGCTCCA TCTGCCACTA CTACGCTCTG CCCCCCCTTC CCAACAAACA CTGCACTGAG
    AAACAGATTC CTCTAGCCGA GATGGAGGCT CTATCTCTGG CATCGGAGAA ATCCTGGTCC
    TTAGCTCTGA CGGATGACTC TGTCCCAGAT GATTTTAACC CCCTCTTACT AACCAGTCAG
    GAGTGCAATA CCACAGATCC TTCAGATCCA CCAGACACCC GGCAGGCGGA TGAACCGACG
    GATGAACACT TGCTATCCGT GAAGAAGACC TCAGTGGGCC GCACACATTC TCTACCTAAT
    GACAGCTACA TGTTCCTGCC GCTGCAGTCC AACTCAAACC ACAGCACCCA CACAACCTCA
    CCTCACCTCG CTCAGACAGG GTCTACAGCT TCTGTTCAGT CTCAAACAGA AGAAGCCAGC
    CAGCACTTGA CGGTGCCCAG CGATCTGTTC AGACCCATCA GTCCTCACAG TTTATCAGAC
    TCAGAGAGCA TCCCTCGAAT TCCCCCACCT CGGCGGGCAC ACACTCTCTC ACGCACACTC
    CGCAGACAGG TGGCAGTGAG TGCTGACTCT CAGGAGACGC TTTACACAGA GGGAGGGGAA
    GCTGAGGGTC CTCTGGGGCT CAGGGAACTG TCGGACCCCC TTCCACCTCA GCAGCAGCAG
    CACCAGCCTT CCCTCTTTCT GGTCCCTGCC ACACCAGGCG CCTCCCCAAA ACCTTCACGA
    CCTAGCGTCC ACACGCAGCA CAACCCCTAC GACCAGTACA ATGTGTTCTC CAGGAGTTCC
    CACTCTCCAC CTGTACCACC ACCACCACCA GACTATAAGA AGCAGGAGGA TGTTGACTCG
    GTGGATCAGG AGGTATCTCG AATTATCCGA GCGGGACTCA CAGGGAGAAG CGATGATGTC
    AACCGAGAAG GGACAGGAGA TCAATGTGCA AAACGTCAGC TTAAGAAATA CCATAGCGTG
    GACACTCAAG GCCAGCGAGC TCTGCTGTTA CCGCGGCCCT TGTCATGGCT TGATGACCCA
    CGCCGGCACT CCATCGAAGT ATGCTCGTCT GTAGAAAGCA GTCCGCAGCG CAGTTCCATA
    TCGTCAGGGT TTGTATCACG AGCCGACAGC CTCCAGCAAC AATCTTCGCC CCGTGGACGC
    AAGAAGAAGA TGAGTCCACC GTGCATTTCT GTAGATCCAC CCGAGGATCC GGAACTTCCG
    GGAGGATTCC ATCCTGCTCT GGGGATTGTG CCACCTCCAT TGCCAAGCCG GGACACTTGC
    CTGAGGAGGC GCGCTCCCTC CAGTGAATCC AAGGACTCGT TTGATTTGGG AGGAGGAGAG
    GCGCTGCCTC AAGAGGGCGT TCCAAACTCC AAGCTATTGA CTCTGCCTAG CTTCTCTTTC
    GAGAAAACAA GCTCTGAGCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214025.2
    CDS784..8127
    Translation

    Target ORF information:

    RefSeq Version XM_017358536.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAGTAAGG AGAAGGAGCT AGCTGAAGCC CAGAGTAAAC CCTTTTACTC CGATTACTGC
    CCAACACGAC GTCTCATCTA CAACATGTGC AAGAGCCAGT ATCTGGACCT GTTCATCACC
    ATCGTCATCG CCCTCAATGT CATCACCATG TCTATGGAGC ACTACCACCA ACCAAAGGTT
    TTGAGCGATT CCCTAAAGAT CTGTAACTAC ATCTTCACAG TCATATTTGT GCTGGAGTCC
    ATCTTCAAAC TTGTTGCTTT TGGATTTCGC CGCTTCTTCA AGGACAGATG GAACCAGTTG
    GATCTGGCCA TTGTTTTGTT GTCTATCATG GGCATAACAC TGGAGGAAAT CGAGGTCAAT
    GCTTCCCTTC CCATCAACCC TACAATCATT CGCATCATGA GGGTGTTGCG CATCACTCGA
    GTTCTAAAGC TACTGAAGAT GGCGGTGGGA ATGCGAGCTC TACTAGACAC TGTGATGCAG
    GCCCTTCCTC AGGTGGGGAA TCTGGGTCTT CTCTTCATGT TGCTTTTCTT CATCTTTGCT
    GCTCTGGGAG TAGAGCTGTT TGGTGACCTG ATATGTGATG AGCTACACCC CTGTGAAGGT
    CTTGGCCGTT ATGCCACGTT CAAGAATTTT GGCATGGCTT TCCTCCTGCT GTTCCGTGTA
    TCCACCGGGG ACAACTGGAA TGGCATCATG AAGGATACGC TCAGAGACTG CTCCCAAGAC
    ACCGGTATAT GCTACAACAC GGTGGTGTCC CCCATTTACT TTGTGTCCTT TGTGCTGACA
    GCTCAGTTTG TGCTGGTCAA TGTGGTGATC GCTGTGCTCA TGAAGCACCT AGAGGAAAGC
    AACAAAGAGG CCAAGGAGGC TGAAGCAGAG GCCGAGCTGG AGCTGGAGCA GGAGATGGAA
    GCTTTGGGTA GAGATAGAGA AATGCAGAGA GGGCACATGC CACGTTTGGG TTCTGGCGAT
    TTGGGTGGGC CAGGAGGGTC ACCTTGGATA TCAAGAGACC ATCGAGACCT GTCAGGACCA
    CTTTACCCCA TAGACAGCCC AACAGCAGAC ATACGCAGAG ATTTTGAAGA TCATTTAAGG
    ACAGATCCTG ATCCACCTCC GAATCTGGAG CCACCATTAG AGCGGAGGCC TATGTTTGAC
    TCAGTTTCCC TGGTGATCCA GGGCTCGCTG GAAGGAGAGC TCAGCCTCAT GGACAACCTG
    TCTGGCTCCA TCTGCCACTA CTACGCTCTG CCCCCCCTTC CCAACAAACA CTGCACTGAG
    AAACAGATTC CTCTAGCCGA GATGGAGGCT CTATCTCTGG CATCGGAGAA ATCCTGGTCC
    TTAGCTCTGA CGGATGACTC TGTCCCAGAT GATTTTAACC CCCTCTTACT AACCAGTCAG
    GAGTGCAATA CCACAGATCC TTCAGATCCA CCAGACACCC GGCAGGCGGA TGAACCGACG
    GATGAACACT TGCTATCCGT GAAGAAGACC TCAGTGGGCC GCACACATTC TCTACCTAAT
    GACAGCTACA TGTTCCTGCC GCTGCAGTCC AACTCAAACC ACAGCACCCA CACAACCTCA
    CCTCACCTCG CTCAGACAGG GTCTACAGCT TCTGTTCAGT CTCAAACAGA AGAAGCCAGC
    CAGCACTTGA CGGTGCCCAG CGATCTGTTC AGACCCATCA GTCCTCACAG TTTATCAGAC
    TCAGAGAGCA TCCCTCGAAT TCCCCCACCT CGGCGGGCAC ACACTCTCTC ACGCACACTC
    CGCAGACAGG TGGCAGTGAG TGCTGACTCT CAGGAGACGC TTTACACAGA GGGAGGGGAA
    GCTGAGGGTC CTCTGGGGCT CAGGGAACTG TCGGACCCCC TTCCACCTCA GCAGCAGCAG
    CACCAGCCTT CCCTCTTTCT GGTCCCTGCC ACACCAGGCG CCTCCCCAAA ACCTTCACGA
    CCTAGCGTCC ACACGCAGCA CAACCCCTAC GACCAGTACA ATGTGTTCTC CAGGAGTTCC
    CACTCTCCAC CTGTACCACC ACCACCACCA GACTATAAGA AGCAGGAGGA TGTTGACTCG
    GTGGATCAGG AGGTATCTCG AATTATCCGA GCGGGACTCA CAGGGAGAAG CGATGATGTC
    AACCGAGAAG GGACAGGAGA TCAATGTGCA AAACGTCAGC TTAAGAAATA CCATAGCGTG
    GACACTCAAG GCCAGCGAGC TCTGCTGTTA CCGCGGCCCT TGTCATGGCT TGATGACCCA
    CGCCGGCACT CCATCGAAGT ATGCTCGTCT GTAGAAAGCA GTCCGCAGCG CAGTTCCATA
    TCGTCAGGGT TTGTATCACG AGCCGACAGC CTCCAGCAAC AATCTTCGCC CCGTGGACGC
    AAGAAGAAGA TGAGTCCACC GTGCATTTCT GTAGATCCAC CCGAGGATCC GGAACTTCCG
    GGAGGATTCC ATCCTGCTCT GGGGATTGTG CCACCTCCAT TGCCAAGCCG GGACACTTGC
    CTGAGGAGGC GCGCTCCCTC CAGTGAATCC AAGGACTCGT TTGATTTGGG AGGAGGAGAG
    GCGCTGCCTC AAGAGGGCGT TCCAAACTCC AAGCTATTGA CTCTGCCTAG CTTCTCTTTC
    GAGAAAACAA GCTCTGAGCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148367
    Clone ID Related Accession (Same CDS sequence) XM_021480348.1
    Accession Version XM_021480348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAAAGCCC AGAGTAAACC CTTTTACTCC GATTACTGCC CAACACGACG TCTCATCTAC
    AACATGTGCA AGAGCCAGTA TCTGGACCTG TTCATCACCA TCGTCATCGC CCTCAATGTC
    ATCACCATGT CTATGGAGCA CTACCACCAA CCAAAGGTTT TGAGCGATTC CCTAAAGATC
    TGTAACTACA TCTTCACAGT CATATTTGTG CTGGAGTCCA TCTTCAAACT TGTTGCTTTT
    GGATTTCGCC GCTTCTTCAA GGACAGATGG AACCAGTTGG ATCTGGCCAT TGTTTTGTTG
    TCTATCATGG GCATAACACT GGAGGAAATC GAGGTCAATG CTTCCCTTCC CATCAACCCT
    ACAATCATTC GCATCATGAG GGTGTTGCGC ATCACTCGAG TTCTAAAGCT ACTGAAGATG
    GCGGTGGGAA TGCGAGCTCT ACTAGACACT GTGATGCAGG CCCTTCCTCA GGTGGGGAAT
    CTGGGTCTTC TCTTCATGTT GCTTTTCTTC ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT
    GGTGACCTGA TATGTGATGA GCTACACCCC TGTGAAGGTC TTGGCCGTTA TGCCACGTTC
    AAGAATTTTG GCATGGCTTT CCTCCTGCTG TTCCGTGTAT CCACCGGGGA CAACTGGAAT
    GGCATCATGA AGGATACGCT CAGAGACTGC TCCCAAGACA CCGGTATATG CTACAACACG
    GTGGTGTCCC CCATTTACTT TGTGTCCTTT GTGCTGACAG CTCAGTTTGT GCTGGTCAAT
    GTGGTGATCG CTGTGCTCAT GAAGCACCTA GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT
    GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG GAGATGGAAG CTTTGGGTAG AGATAGAGAA
    ATGCAGAGAG GGCACATGCC ACGTTTGGGT TCTGGCGATT TGGGTGGGCC AGGAGGGTCA
    CCTTGGATAT CAAGAGACCA TCGAGACCTG TCAGGACCAC TTTACCCCAT AGACAGCCCA
    ACAGCAGACA TACGCAGAGA TTTTGAAGAT CATTTAAGGA CAGATCCTGA TCCACCTCCG
    AATCTGGAGC CACCATTAGA GCGGAGGCCT ATGTTTGACT CAGTTTCCCT GGTGATCCAG
    GGCTCGCTGG AAGGAGAGCT CAGCCTCATG GACAACCTGT CTGGCTCCAT CTGCCACTAC
    TACGCTCTGC CCCCCCTTCC CAACAAACAC TGCACTGAGA AACAGATTCC TCTAGCCGAG
    ATGGAGGCTC TATCTCTGGC ATCGGAGAAA TCCTGGTCCT TAGCTCTGAC GGATGACTCT
    GTCCCAGATG ATTTTAACCC CCTCTTACTA ACCAGTCAGG AGTGCAATAC CACAGATCCT
    TCAGATCCAC CAGACACCCG GCAGGCGGAT GAACCGACGG ATGAACACTT GCTATCCGTG
    AAGAAGACCT CAGTGGGCCG CACACATTCT CTACCTAATG ACAGCTACAT GTTCCTGCCG
    CTGCAGTCCA ACTCAAACCA CAGCACCCAC ACAACCTCAC CTCACCTCGC TCAGACAGGG
    TCTACAGCTT CTGTTCAGTC TCAAACAGAA GAAGCCAGCC AGCACTTGAC GGTGCCCAGC
    GATCTGTTCA GACCCATCAG TCCTCACAGT TTATCAGACT CAGAGAGCAT CCCTCGAATT
    CCCCCACCTC GGCGGGCACA CACTCTCTCA CGCACACTCC GCAGACAGGT GGCAGTGAGT
    GCTGACTCTC AGGAGACGCT TTACACAGAG GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC
    AGGGAACTGT CGGACCCCCT TCCACCTCAG CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG
    GTCCCTGCCA CACCAGGCGC CTCCCCAAAA CCTTCACGAC CTAGCGTCCA CACGCAGCAC
    AACCCCTACG ACCAGTACAA TGTGTTCTCC AGGAGTTCCC ACTCTCCACC TGTACCACCA
    CCACCACCAG ACTATAAGAA GCAGGAGGAT GTTGACTCGG TGGATCAGGA GGTATCTCGA
    ATTATCCGAG CGGGACTCAC AGGGAGAAGC GATGATGTCA ACCGAGAAGG GACAGGAGAT
    CAATGTGCAA AACGTCAGCT TAAGAAATAC CATAGCGTGG ACACTCAAGG CCAGCGAGCT
    CTGCTGTTAC CGCGGCCCTT GTCATGGCTT GATGACCCAC GCCGGCACTC CATCGAAGTA
    TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC AGTTCCATAT CGTCAGGGTT TGTATCACGA
    GCCGACAGCC TCCAGCAACA ATCTTCGCCC CGTGGACGCA AGAAGAAGAT GAGTCCACCG
    TGCATTTCTG TAGATCCACC CGAGGATCCG GAACTTCCGG GAGGATTCCA TCCTGCTCTG
    GGGATTGTGC CACCTCCATT GCCAAGCCGG GACACTTGCC TGAGGAGGCG CGCTCCCTCC
    AGTGAATCCA AGGACTCGTT TGATTTGGGA GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT
    CCAAACTCCA AGCTATTGAC TCTGCCTAGC TTCTCTTTCG AGAAAACAAG CTCTGAGCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336023.1
    CDS783..8105
    Translation

    Target ORF information:

    RefSeq Version XM_021480348.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC
    TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG
    TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG
    CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA
    GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC
    CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT
    AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG
    CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT
    GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC
    GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG
    CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC
    AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA
    GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA
    GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG
    TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG
    ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT
    CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC
    ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA
    AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC
    ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC
    AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG
    CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG
    TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG
    ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA
    AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC
    AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT
    TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG
    AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC
    GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG
    GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA
    AGGAAAGCCC AGAGTAAACC CTTTTACTCC GATTACTGCC CAACACGACG TCTCATCTAC
    AACATGTGCA AGAGCCAGTA TCTGGACCTG TTCATCACCA TCGTCATCGC CCTCAATGTC
    ATCACCATGT CTATGGAGCA CTACCACCAA CCAAAGGTTT TGAGCGATTC CCTAAAGATC
    TGTAACTACA TCTTCACAGT CATATTTGTG CTGGAGTCCA TCTTCAAACT TGTTGCTTTT
    GGATTTCGCC GCTTCTTCAA GGACAGATGG AACCAGTTGG ATCTGGCCAT TGTTTTGTTG
    TCTATCATGG GCATAACACT GGAGGAAATC GAGGTCAATG CTTCCCTTCC CATCAACCCT
    ACAATCATTC GCATCATGAG GGTGTTGCGC ATCACTCGAG TTCTAAAGCT ACTGAAGATG
    GCGGTGGGAA TGCGAGCTCT ACTAGACACT GTGATGCAGG CCCTTCCTCA GGTGGGGAAT
    CTGGGTCTTC TCTTCATGTT GCTTTTCTTC ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT
    GGTGACCTGA TATGTGATGA GCTACACCCC TGTGAAGGTC TTGGCCGTTA TGCCACGTTC
    AAGAATTTTG GCATGGCTTT CCTCCTGCTG TTCCGTGTAT CCACCGGGGA CAACTGGAAT
    GGCATCATGA AGGATACGCT CAGAGACTGC TCCCAAGACA CCGGTATATG CTACAACACG
    GTGGTGTCCC CCATTTACTT TGTGTCCTTT GTGCTGACAG CTCAGTTTGT GCTGGTCAAT
    GTGGTGATCG CTGTGCTCAT GAAGCACCTA GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT
    GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG GAGATGGAAG CTTTGGGTAG AGATAGAGAA
    ATGCAGAGAG GGCACATGCC ACGTTTGGGT TCTGGCGATT TGGGTGGGCC AGGAGGGTCA
    CCTTGGATAT CAAGAGACCA TCGAGACCTG TCAGGACCAC TTTACCCCAT AGACAGCCCA
    ACAGCAGACA TACGCAGAGA TTTTGAAGAT CATTTAAGGA CAGATCCTGA TCCACCTCCG
    AATCTGGAGC CACCATTAGA GCGGAGGCCT ATGTTTGACT CAGTTTCCCT GGTGATCCAG
    GGCTCGCTGG AAGGAGAGCT CAGCCTCATG GACAACCTGT CTGGCTCCAT CTGCCACTAC
    TACGCTCTGC CCCCCCTTCC CAACAAACAC TGCACTGAGA AACAGATTCC TCTAGCCGAG
    ATGGAGGCTC TATCTCTGGC ATCGGAGAAA TCCTGGTCCT TAGCTCTGAC GGATGACTCT
    GTCCCAGATG ATTTTAACCC CCTCTTACTA ACCAGTCAGG AGTGCAATAC CACAGATCCT
    TCAGATCCAC CAGACACCCG GCAGGCGGAT GAACCGACGG ATGAACACTT GCTATCCGTG
    AAGAAGACCT CAGTGGGCCG CACACATTCT CTACCTAATG ACAGCTACAT GTTCCTGCCG
    CTGCAGTCCA ACTCAAACCA CAGCACCCAC ACAACCTCAC CTCACCTCGC TCAGACAGGG
    TCTACAGCTT CTGTTCAGTC TCAAACAGAA GAAGCCAGCC AGCACTTGAC GGTGCCCAGC
    GATCTGTTCA GACCCATCAG TCCTCACAGT TTATCAGACT CAGAGAGCAT CCCTCGAATT
    CCCCCACCTC GGCGGGCACA CACTCTCTCA CGCACACTCC GCAGACAGGT GGCAGTGAGT
    GCTGACTCTC AGGAGACGCT TTACACAGAG GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC
    AGGGAACTGT CGGACCCCCT TCCACCTCAG CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG
    GTCCCTGCCA CACCAGGCGC CTCCCCAAAA CCTTCACGAC CTAGCGTCCA CACGCAGCAC
    AACCCCTACG ACCAGTACAA TGTGTTCTCC AGGAGTTCCC ACTCTCCACC TGTACCACCA
    CCACCACCAG ACTATAAGAA GCAGGAGGAT GTTGACTCGG TGGATCAGGA GGTATCTCGA
    ATTATCCGAG CGGGACTCAC AGGGAGAAGC GATGATGTCA ACCGAGAAGG GACAGGAGAT
    CAATGTGCAA AACGTCAGCT TAAGAAATAC CATAGCGTGG ACACTCAAGG CCAGCGAGCT
    CTGCTGTTAC CGCGGCCCTT GTCATGGCTT GATGACCCAC GCCGGCACTC CATCGAAGTA
    TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC AGTTCCATAT CGTCAGGGTT TGTATCACGA
    GCCGACAGCC TCCAGCAACA ATCTTCGCCC CGTGGACGCA AGAAGAAGAT GAGTCCACCG
    TGCATTTCTG TAGATCCACC CGAGGATCCG GAACTTCCGG GAGGATTCCA TCCTGCTCTG
    GGGATTGTGC CACCTCCATT GCCAAGCCGG GACACTTGCC TGAGGAGGCG CGCTCCCTCC
    AGTGAATCCA AGGACTCGTT TGATTTGGGA GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT
    CCAAACTCCA AGCTATTGAC TCTGCCTAGC TTCTCTTTCG AGAAAACAAG CTCTGAGCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148368
    Clone ID Related Accession (Same CDS sequence) XM_021480349.1
    Accession Version XM_021480349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG ACCACAGATC CTTCAGATCC ACCAGACACC
    CGGCAGGCGG ATGAACCGAC GGATGAACAC TTGCTATCCG TGAAGAAGAC CTCAGTGGGC
    CGCACACATT CTCTACCTAA TGACAGCTAC ATGTTCCTGC CGCTGCAGTC CAACTCAAAC
    CACAGCACCC ACACAACCTC ACCTCACCTC GCTCAGACAG GGTCTACAGC TTCTGTTCAG
    TCTCAAACAG AAGAAGCCAG CCAGCACTTG ACGGTGCCCA GCGATCTGTT CAGACCCATC
    AGTCCTCACA GTTTATCAGA CTCAGAGAGC ATCCCTCGAA TTCCCCCACC TCGGCGGGCA
    CACACTCTCT CACGCACACT CCGCAGACAG GTGGCAGTGA GTGCTGACTC TCAGGAGACG
    CTTTACACAG AGGGAGGGGA AGCTGAGGGT CCTCTGGGGC TCAGGGAACT GTCGGACCCC
    CTTCCACCTC AGCAGCAGCA GCACCAGCCT TCCCTCTTTC TGGTCCCTGC CACACCAGGC
    GCCTCCCCAA AACCTTCACG ACCTAGCGTC CACACGCAGC ACAACCCCTA CGACCAGTAC
    AATGTGTTCT CCAGGAGTTC CCACTCTCCA CCTGTACCAC CACCACCACC AGACTATAAG
    AAGCAGGAGG ATGTTGACTC GGTGGATCAG GAGGTATCTC GAATTATCCG AGCGGGACTC
    ACAGGGAGAA GCGATGATGT CAACCGAGAA GGGACAGGAG ATCAATGTGC AAAACGTCAG
    CTTAAGAAAT ACCATAGCGT GGACACTCAA GGCCAGCGAG CTCTGCTGTT ACCGCGGCCC
    TTGTCATGGC TTGATGACCC ACGCCGGCAC TCCATCGAAG TATGCTCGTC TGTAGAAAGC
    AGTCCGCAGC GCAGTTCCAT ATCGTCAGGG TTTGTATCAC GAGCCGACAG CCTCCAGCAA
    CAATCTTCGC CCCGTGGACG CAAGAAGAAG ATGAGTCCAC CGTGCATTTC TGTAGATCCA
    CCCGAGGATC CGGAACTTCC GGGAGGATTC CATCCTGCTC TGGGGATTGT GCCACCTCCA
    TTGCCAAGCC GGGACACTTG CCTGAGGAGG CGCGCTCCCT CCAGTGAATC CAAGGACTCG
    TTTGATTTGG GAGGAGGAGA GGCGCTGCCT CAAGAGGGCG TTCCAAACTC CAAGCTATTG
    ACTCTGCCTA GCTTCTCTTT CGAGAAAACA AGCTCTGAGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336024.1
    CDS783..8027
    Translation

    Target ORF information:

    RefSeq Version XM_021480349.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
    AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC
    AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG
    GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT
    TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG
    TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT
    CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT
    GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC
    TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG
    GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA
    GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG
    ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT
    TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC
    TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA
    GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG
    TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT
    TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC
    GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG
    ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG
    GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC
    TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA
    GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC
    AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC
    CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG
    AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG
    CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT
    CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG
    AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG
    CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT
    CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT
    CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT
    TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC
    CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT
    CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC
    ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC
    AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT
    GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA
    TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC
    AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA
    CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA
    AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT
    GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC
    AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG
    CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC
    CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC
    ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA
    GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT
    CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT
    CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC
    CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT
    TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA
    GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA
    GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT
    TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT
    CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC
    CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT
    GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT
    GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC
    CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT
    GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT
    GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG
    CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG
    CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC
    GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA
    GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG
    GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT
    CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT
    GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC
    GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC
    TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC
    TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC
    ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG
    CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG
    GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT
    GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG
    TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC
    AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT
    GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT
    GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC
    TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT
    CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG
    AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT
    GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC
    ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG ACCACAGATC CTTCAGATCC ACCAGACACC
    CGGCAGGCGG ATGAACCGAC GGATGAACAC TTGCTATCCG TGAAGAAGAC CTCAGTGGGC
    CGCACACATT CTCTACCTAA TGACAGCTAC ATGTTCCTGC CGCTGCAGTC CAACTCAAAC
    CACAGCACCC ACACAACCTC ACCTCACCTC GCTCAGACAG GGTCTACAGC TTCTGTTCAG
    TCTCAAACAG AAGAAGCCAG CCAGCACTTG ACGGTGCCCA GCGATCTGTT CAGACCCATC
    AGTCCTCACA GTTTATCAGA CTCAGAGAGC ATCCCTCGAA TTCCCCCACC TCGGCGGGCA
    CACACTCTCT CACGCACACT CCGCAGACAG GTGGCAGTGA GTGCTGACTC TCAGGAGACG
    CTTTACACAG AGGGAGGGGA AGCTGAGGGT CCTCTGGGGC TCAGGGAACT GTCGGACCCC
    CTTCCACCTC AGCAGCAGCA GCACCAGCCT TCCCTCTTTC TGGTCCCTGC CACACCAGGC
    GCCTCCCCAA AACCTTCACG ACCTAGCGTC CACACGCAGC ACAACCCCTA CGACCAGTAC
    AATGTGTTCT CCAGGAGTTC CCACTCTCCA CCTGTACCAC CACCACCACC AGACTATAAG
    AAGCAGGAGG ATGTTGACTC GGTGGATCAG GAGGTATCTC GAATTATCCG AGCGGGACTC
    ACAGGGAGAA GCGATGATGT CAACCGAGAA GGGACAGGAG ATCAATGTGC AAAACGTCAG
    CTTAAGAAAT ACCATAGCGT GGACACTCAA GGCCAGCGAG CTCTGCTGTT ACCGCGGCCC
    TTGTCATGGC TTGATGACCC ACGCCGGCAC TCCATCGAAG TATGCTCGTC TGTAGAAAGC
    AGTCCGCAGC GCAGTTCCAT ATCGTCAGGG TTTGTATCAC GAGCCGACAG CCTCCAGCAA
    CAATCTTCGC CCCGTGGACG CAAGAAGAAG ATGAGTCCAC CGTGCATTTC TGTAGATCCA
    CCCGAGGATC CGGAACTTCC GGGAGGATTC CATCCTGCTC TGGGGATTGT GCCACCTCCA
    TTGCCAAGCC GGGACACTTG CCTGAGGAGG CGCGCTCCCT CCAGTGAATC CAAGGACTCG
    TTTGATTTGG GAGGAGGAGA GGCGCTGCCT CAAGAGGGCG TTCCAAACTC CAAGCTATTG
    ACTCTGCCTA GCTTCTCTTT CGAGAAAACA AGCTCTGAGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54519
    Clone ID Related Accession (Same CDS sequence) XM_017358537.1
    Accession Version XM_017358537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTGGTGA TCCTGCTGAA CTGTGTTACT CTGGGGATGT TTCACCCGTG CGAGGACAGC 
    CACTGTGACT CAGAGCGATG CAAGATACTG GAGGACTTTG ATGATTTCAT CTTTGCTTTT
    TTTGCCGTGG AGATGGTAAT CAAGATGGTT GCACTGGGTA TCTTTGGGAA AAAGTGTTAC
    CTTGGAGACA CCTGGAATCG GCTAGACTTC TTCATTGTGT TGGCTGGGAT GCTGGAATAT
    TCTCTCAACC TGCAGAATGT TAGTTTCTCT GCGGTCCGAA CAGTCAGAGT ACTTCGACCT
    CTTCGGGCTA TCAACCGAGT TCCCAGTATG AGAATCCTGG TGACGCTTCT ACTTGACACT
    CTCCCCATGC TGGGCAATGT GCTGCTGCTT TGCTTCTTTG TTTTCTTCAT CTTTGGGATC
    GTTGGAGTTC AGCTGTGGGC CGGGCTTCTC CGCAACCGCT GCTTCTTACC TGAAAACTTC
    TCCCTACCTA CCAGCCTAGA ACTTCGCAAG TACTACCACA CAGAAAACGA CGATGAAAAC
    CCGTTTATCT GCTCCCAACC AAGGGAGAAC GGCATGCGTC TGTGCACCTC CGTCCCCACG
    CTCCATGAGG AGGGCCGGCA GTGCCAGCTG GACATGGCAT CTTACAATAG CACAGACAAC
    ACCACCTGCG TCAACTGGAA CAAGTATTAC ACCAACTGCT CCGCCGGAGA GGCCAATCCC
    TTCAAGGGGG CCATAAACTT TGATAACATT GGATATGCCT GGATTGCCAT CTTTCAGGTT
    ATTACTCTGG AGGGCTGGGT GGACATCATG TACTTTGTTA TGGATGCTCA CTCCTTCTAC
    AATTTCATAT ACTTCATCCT CCTGATAATA GTGGGCTCGT TCTTCATGAT CAACCTTTGT
    CTAGTTGTCA TAGCGACACA GTTCTCCGAG ACCAAGCAAC GAGAGAGTCA GCTGATGAAG
    GAGCAGCGAG TTCGCTTTAG GTCCAACGCC AGCACTCTCA ACAGCTACTC CGAGCCTGGA
    AGCTGCTATG ATGAGCTGCT CAAATACCTG GTCCACGTCA TCCGCAAAGG CACCAGACAA
    ATCGCTCACC TCATCCGGGC TGCGGGTCGC CGTGCAGGCC TGAGGATCTG TGCTTCTCCA
    CCTCTGGAAG CACCACCCAC TAAAAGACGT CGGCAAAAGC AGCGTCAGGG ATCTATTCGT
    CACATCATGC ACCATCACCA CCACCACCTC CACCATCACT ATCATTTGGG CAATGGGAGT
    GTCCGATCTG ACGGGGGACG AGAGGTGGAC AATCCTTCGC AGAGTGGCAT TGTCAACAAA
    AGTGGCAATG TCAATCTAAT GCTACCTGTT ATTGTCCCTC AGCAGGACGC CTTTAGTGGG
    TCTTTTGGCC ACAGTAGTGC CGAATCGGTA CATAGTGTTT ATCAGGTCTC GGGTCACCTT
    GAGCCACTTC GCTGTGGCCC TTCACCGTCT CCCACAGTTC TTCATGCGTA TAAGAGGAAC
    TCAGTGCCCT TTGCAGCTCC AGTACATAAG AACTACCCCA CTTTACAGTC ATCTCTGGCC
    TTAGAGCAGC TCAGACAGAG AATCCTGGAT CCTGCTAGTG TCCTTACCAG CCTGAACATA
    CCACCAAATC CCATCAACAC CTCACAGTGC CTGGTGGAGA CTCAAGGTCC TCCTGGCAAG
    GTCATCTCAA AGGATGCCCT CATGAACTGC AGTAGCACAA ACATAGACAC AGCTCTGACC
    TATGACCCTG AAACCTGTCC ATATTGTGCC AAATCAATGG CCAATGACTC TGAGGGAATA
    GATGGTAATG AGACGGCAGA CTCTGACAGT GAGGGTGTCT ATGAGACACA AGTTGCCCAT
    TATCGGGACA GCAGAGGACC CAAGAAGAAG AAAAAGTTTG CATTAGGAGC TCGGGCAACA
    AAGGTGATCG ATTTCTGGAG ACTGGTCTGT GGCACTTTCC GCAAGATTGT GGACAGCAAG
    TATTTTGGAC AAGGAATAAT GATCGCCATT CTTATTAACA CACTCAGCAT GGGCATTGAG
    TACCATGAAC AGCCTGATGA GTTGACAAAT GCTCTGGAAA TAAGCAACAT CGTCTTCACC
    AGTTTGTTTG TGCTGGAGAT GCTCCTGAAA CTGCTAGTCT ATGGGCCTTT TGGGTACATC
    AAGAACCCGT ACAACATCTT TGACGGCATT ATTGTTGTAA TCAGTGTTTG GGAGATTGTT
    GGCCAGCAGG GAGGAGGTTT ATCGGTGCTG AGGACCTTCA GGCTGATGCG GGTGTTGAAG
    TTGGTCCGAT TTATGCCCGC CTTGCAGAGG CAGTTGGTGG TCCTCATGAA GACCATGGAC
    AATGTTGCCA CCTTCTGCAT GCTTCTGATG CTTTTCATAT TCATTTTCAG CATTCTAGGC
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGAAATTACC AAGCGGGACG ATTTGCATGG CCAGCTGAGC
    TGTATTCAGC TGCCTATAGA CTCAGGGGGT GATGCCTCAA AGTCGGACTC TGAAGCCGAT
    ATTTATGCAC GGAGCTTGGA GGATGTTTCG GGACACAAGA AAGATATGTC TGCATCATCC
    GTTGTGCCCA TCAATGGCCA TGTGGATCTG AAGAGCAGCC TGACACCGCC TCTGATCACC
    CACACAGCTG CCACACCAAT GCCCATCCCC AAGATATCAG GAGGAGGAGA TTCAGCACTG
    AGCCAAGAGT CCCGCAGAGG CAGCAGTGTC TCCATGGAGC CTAATGGCCA AGACAAATCT
    CCATCCAGTG CACGGAGCTC CCCTAATGCC CCCTTGAGCT CCGCCAGTAG CTGGAACAGC
    AGACGCTCCA GCTGGAACAG TCTAGGCCGA GCCCCCAGCC TCAAGAGGCA GAAACACCAG
    TCTGGTGAAA GGCGCTCCTT GTTGTCTGGT GATGGCCAGT CCAGTTCTGA TGAGGGGGAT
    GCAGGAGAGA GCATGGGGGG CATGTCTGAG GGTGATGATG CCTCCCTCGC CAGGACGGAC
    TCGCTGGGCC AGAGGCCTCG ACATAGGCGC ATGGAGTCCC TGGAGACGCG CAGCTCATTT
    GATCTCCATC CAGACACCCT GCAGGTGCCG TACCCCCACC GCTCCGGCAG CATACACAGC
    GCACGGCCAC CCAACTTCCT CAGCAACGGC AAGACTTCTC CATCTGCAGC CACCACACAG
    CTCTCTTTGG ATGAACACCA CAGTGAAGAC GACAACGCAG ACGAGGAAGG AAACCTGAGT
    CGCAGGGCTC GTCTGTACAG ATGGCTGGAG CACAAGCAGC CCGAATGGTG TCGGCAGAGG
    GTCACCTGGT CCCTTTACCT CTTTCCTCCA GAGTCACGGT TTCGCGTGAC ATGCAATAAA
    ATCATCACCC ACAAGATGTT TGACCATGTG GTTCTTGTCA TCATCTTTCT CAACTGCATC
    ACCATCGCCA TGGAGAGGCC CAGAATCGAC CCCTCTAGCG CTGAACGCAT CTTCTTGACG
    TTGTCCAACT ACATATTTAC TGCCATCTTT GTGACCGAAA TGACAATAAA GGTGGTTGCT
    TTGGGCTTTT GTTTTGGAGA GAAGACCTAC CTGAAGAGCA GCTGGAACAT TCTGGATGGC
    ATGCTGGTGA TGATCTCTGT GATTGACATC CTCGTGTCCC TGATCTCCAA CAGTGGCACC
    AAGATCCTGG GCATGTTACG TGTGCTGCGG TTACTGAGGA CACTCAGGCC ACTCAGAGTG
    ATCAGCAGAG CTCCTGGACT GAAACTGGTT GTTGAGACAT TGATGTCGTC TCTTAAGCCT
    ATCGGAAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATTA TTTTTGGGAT CTTGGGAGTT
    CAGCTTTTCA AGGGAAAGTT CTTTGTGTGT CATGGGGATG ATACGAGAAA CATCACCAAC
    AAGTCAGATT GCCTCCTGGC AAACTACAAA TGGGTCAGAC ACAAGTACAA CTTTGATAAC
    CTTGGACAGG CCCTCATGTC CCTTTTTGTT CTGGCTTCCA AAGACGGTTG GGTGGATATC
    ATGTACGATG GACTGGATGC TGTCGGTGTG GACCAGCAGC CCGTCATGAA TTACAACCCA
    TGGATGCTGC TCTATTTTAT CTCCTTCTTG CTGATCGTGG CTTTCTTCGT GCTCAACATG
    TTTGTCGGCG TGGTCGTCGA GAACTTTCAC AAGTGCAGGA GAAACCAGGA GGAGGAAGAA
    GCCAAACGGC GAGAAGCGAA GCGCCAGAAA CGACGTGATA AAAAACGAAG GAGTAAGGAG
    AAGGAGCTAG CTGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAACGC TTCCCTTCCC
    ATCAATCCTA CCATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AAAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT
    CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG
    GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC
    ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG
    CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG
    TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT
    CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG
    GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC
    CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG
    GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT
    CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC
    CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC
    ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT
    GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG
    GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG
    ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC
    CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC
    ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT
    GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG
    AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT
    CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC
    GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA
    GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC
    TCTGAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214026.1
    CDS15..7106
    Translation

    Target ORF information:

    RefSeq Version XM_017358537.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTGGTGA TCCTGCTGAA CTGTGTTACT CTGGGGATGT TTCACCCGTG CGAGGACAGC 
    CACTGTGACT CAGAGCGATG CAAGATACTG GAGGACTTTG ATGATTTCAT CTTTGCTTTT
    TTTGCCGTGG AGATGGTAAT CAAGATGGTT GCACTGGGTA TCTTTGGGAA AAAGTGTTAC
    CTTGGAGACA CCTGGAATCG GCTAGACTTC TTCATTGTGT TGGCTGGGAT GCTGGAATAT
    TCTCTCAACC TGCAGAATGT TAGTTTCTCT GCGGTCCGAA CAGTCAGAGT ACTTCGACCT
    CTTCGGGCTA TCAACCGAGT TCCCAGTATG AGAATCCTGG TGACGCTTCT ACTTGACACT
    CTCCCCATGC TGGGCAATGT GCTGCTGCTT TGCTTCTTTG TTTTCTTCAT CTTTGGGATC
    GTTGGAGTTC AGCTGTGGGC CGGGCTTCTC CGCAACCGCT GCTTCTTACC TGAAAACTTC
    TCCCTACCTA CCAGCCTAGA ACTTCGCAAG TACTACCACA CAGAAAACGA CGATGAAAAC
    CCGTTTATCT GCTCCCAACC AAGGGAGAAC GGCATGCGTC TGTGCACCTC CGTCCCCACG
    CTCCATGAGG AGGGCCGGCA GTGCCAGCTG GACATGGCAT CTTACAATAG CACAGACAAC
    ACCACCTGCG TCAACTGGAA CAAGTATTAC ACCAACTGCT CCGCCGGAGA GGCCAATCCC
    TTCAAGGGGG CCATAAACTT TGATAACATT GGATATGCCT GGATTGCCAT CTTTCAGGTT
    ATTACTCTGG AGGGCTGGGT GGACATCATG TACTTTGTTA TGGATGCTCA CTCCTTCTAC
    AATTTCATAT ACTTCATCCT CCTGATAATA GTGGGCTCGT TCTTCATGAT CAACCTTTGT
    CTAGTTGTCA TAGCGACACA GTTCTCCGAG ACCAAGCAAC GAGAGAGTCA GCTGATGAAG
    GAGCAGCGAG TTCGCTTTAG GTCCAACGCC AGCACTCTCA ACAGCTACTC CGAGCCTGGA
    AGCTGCTATG ATGAGCTGCT CAAATACCTG GTCCACGTCA TCCGCAAAGG CACCAGACAA
    ATCGCTCACC TCATCCGGGC TGCGGGTCGC CGTGCAGGCC TGAGGATCTG TGCTTCTCCA
    CCTCTGGAAG CACCACCCAC TAAAAGACGT CGGCAAAAGC AGCGTCAGGG ATCTATTCGT
    CACATCATGC ACCATCACCA CCACCACCTC CACCATCACT ATCATTTGGG CAATGGGAGT
    GTCCGATCTG ACGGGGGACG AGAGGTGGAC AATCCTTCGC AGAGTGGCAT TGTCAACAAA
    AGTGGCAATG TCAATCTAAT GCTACCTGTT ATTGTCCCTC AGCAGGACGC CTTTAGTGGG
    TCTTTTGGCC ACAGTAGTGC CGAATCGGTA CATAGTGTTT ATCAGGTCTC GGGTCACCTT
    GAGCCACTTC GCTGTGGCCC TTCACCGTCT CCCACAGTTC TTCATGCGTA TAAGAGGAAC
    TCAGTGCCCT TTGCAGCTCC AGTACATAAG AACTACCCCA CTTTACAGTC ATCTCTGGCC
    TTAGAGCAGC TCAGACAGAG AATCCTGGAT CCTGCTAGTG TCCTTACCAG CCTGAACATA
    CCACCAAATC CCATCAACAC CTCACAGTGC CTGGTGGAGA CTCAAGGTCC TCCTGGCAAG
    GTCATCTCAA AGGATGCCCT CATGAACTGC AGTAGCACAA ACATAGACAC AGCTCTGACC
    TATGACCCTG AAACCTGTCC ATATTGTGCC AAATCAATGG CCAATGACTC TGAGGGAATA
    GATGGTAATG AGACGGCAGA CTCTGACAGT GAGGGTGTCT ATGAGACACA AGTTGCCCAT
    TATCGGGACA GCAGAGGACC CAAGAAGAAG AAAAAGTTTG CATTAGGAGC TCGGGCAACA
    AAGGTGATCG ATTTCTGGAG ACTGGTCTGT GGCACTTTCC GCAAGATTGT GGACAGCAAG
    TATTTTGGAC AAGGAATAAT GATCGCCATT CTTATTAACA CACTCAGCAT GGGCATTGAG
    TACCATGAAC AGCCTGATGA GTTGACAAAT GCTCTGGAAA TAAGCAACAT CGTCTTCACC
    AGTTTGTTTG TGCTGGAGAT GCTCCTGAAA CTGCTAGTCT ATGGGCCTTT TGGGTACATC
    AAGAACCCGT ACAACATCTT TGACGGCATT ATTGTTGTAA TCAGTGTTTG GGAGATTGTT
    GGCCAGCAGG GAGGAGGTTT ATCGGTGCTG AGGACCTTCA GGCTGATGCG GGTGTTGAAG
    TTGGTCCGAT TTATGCCCGC CTTGCAGAGG CAGTTGGTGG TCCTCATGAA GACCATGGAC
    AATGTTGCCA CCTTCTGCAT GCTTCTGATG CTTTTCATAT TCATTTTCAG CATTCTAGGC
    ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA
    AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG
    GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC
    TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA
    GTGGAGGGAT TCCAAACAGA GGAAATTACC AAGCGGGACG ATTTGCATGG CCAGCTGAGC
    TGTATTCAGC TGCCTATAGA CTCAGGGGGT GATGCCTCAA AGTCGGACTC TGAAGCCGAT
    ATTTATGCAC GGAGCTTGGA GGATGTTTCG GGACACAAGA AAGATATGTC TGCATCATCC
    GTTGTGCCCA TCAATGGCCA TGTGGATCTG AAGAGCAGCC TGACACCGCC TCTGATCACC
    CACACAGCTG CCACACCAAT GCCCATCCCC AAGATATCAG GAGGAGGAGA TTCAGCACTG
    AGCCAAGAGT CCCGCAGAGG CAGCAGTGTC TCCATGGAGC CTAATGGCCA AGACAAATCT
    CCATCCAGTG CACGGAGCTC CCCTAATGCC CCCTTGAGCT CCGCCAGTAG CTGGAACAGC
    AGACGCTCCA GCTGGAACAG TCTAGGCCGA GCCCCCAGCC TCAAGAGGCA GAAACACCAG
    TCTGGTGAAA GGCGCTCCTT GTTGTCTGGT GATGGCCAGT CCAGTTCTGA TGAGGGGGAT
    GCAGGAGAGA GCATGGGGGG CATGTCTGAG GGTGATGATG CCTCCCTCGC CAGGACGGAC
    TCGCTGGGCC AGAGGCCTCG ACATAGGCGC ATGGAGTCCC TGGAGACGCG CAGCTCATTT
    GATCTCCATC CAGACACCCT GCAGGTGCCG TACCCCCACC GCTCCGGCAG CATACACAGC
    GCACGGCCAC CCAACTTCCT CAGCAACGGC AAGACTTCTC CATCTGCAGC CACCACACAG
    CTCTCTTTGG ATGAACACCA CAGTGAAGAC GACAACGCAG ACGAGGAAGG AAACCTGAGT
    CGCAGGGCTC GTCTGTACAG ATGGCTGGAG CACAAGCAGC CCGAATGGTG TCGGCAGAGG
    GTCACCTGGT CCCTTTACCT CTTTCCTCCA GAGTCACGGT TTCGCGTGAC ATGCAATAAA
    ATCATCACCC ACAAGATGTT TGACCATGTG GTTCTTGTCA TCATCTTTCT CAACTGCATC
    ACCATCGCCA TGGAGAGGCC CAGAATCGAC CCCTCTAGCG CTGAACGCAT CTTCTTGACG
    TTGTCCAACT ACATATTTAC TGCCATCTTT GTGACCGAAA TGACAATAAA GGTGGTTGCT
    TTGGGCTTTT GTTTTGGAGA GAAGACCTAC CTGAAGAGCA GCTGGAACAT TCTGGATGGC
    ATGCTGGTGA TGATCTCTGT GATTGACATC CTCGTGTCCC TGATCTCCAA CAGTGGCACC
    AAGATCCTGG GCATGTTACG TGTGCTGCGG TTACTGAGGA CACTCAGGCC ACTCAGAGTG
    ATCAGCAGAG CTCCTGGACT GAAACTGGTT GTTGAGACAT TGATGTCGTC TCTTAAGCCT
    ATCGGAAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATTA TTTTTGGGAT CTTGGGAGTT
    CAGCTTTTCA AGGGAAAGTT CTTTGTGTGT CATGGGGATG ATACGAGAAA CATCACCAAC
    AAGTCAGATT GCCTCCTGGC AAACTACAAA TGGGTCAGAC ACAAGTACAA CTTTGATAAC
    CTTGGACAGG CCCTCATGTC CCTTTTTGTT CTGGCTTCCA AAGACGGTTG GGTGGATATC
    ATGTACGATG GACTGGATGC TGTCGGTGTG GACCAGCAGC CCGTCATGAA TTACAACCCA
    TGGATGCTGC TCTATTTTAT CTCCTTCTTG CTGATCGTGG CTTTCTTCGT GCTCAACATG
    TTTGTCGGCG TGGTCGTCGA GAACTTTCAC AAGTGCAGGA GAAACCAGGA GGAGGAAGAA
    GCCAAACGGC GAGAAGCGAA GCGCCAGAAA CGACGTGATA AAAAACGAAG GAGTAAGGAG
    AAGGAGCTAG CTGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT
    CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC
    CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC
    CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT
    GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT
    GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAACGC TTCCCTTCCC
    ATCAATCCTA CCATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA
    CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG
    GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA
    GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT
    GCCACGTTCA AAAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC
    AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC
    TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG
    CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC
    AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA
    GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA
    GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA
    GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT
    CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG
    GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC
    TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT
    CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG
    GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC
    ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG
    CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG
    TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT
    CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG
    GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC
    CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG
    GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT
    CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC
    CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC
    ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT
    GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG
    GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG
    ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC
    CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC
    ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT
    GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG
    AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT
    CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC
    GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA
    GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC
    TCTGAGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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