nf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nf1b
Entrez Gene ID	564518
Full Name	neurofibromin 1b
Synonyms	si:dkey-15g19.1
General protein information	Preferred Names neurofibromin 1b Names neurofibromin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169189.3	XP_005169246.1	neurofibromin isoform X1
XM_005169189.4	XP_005169246.1	neurofibromin isoform X1
XM_005169190.3	XP_005169247.1	neurofibromin isoform X2
XM_017358082.1	XP_017213571.1	neurofibromin isoform X3
XM_005169190.4	XP_005169247.1	neurofibromin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NF1	NP_001035957.1
Rattus norvegicus (Norway rat)	Nf1	NP_036741.1
Danio rerio (zebrafish)	nf1b	XP_692937.4
Drosophila melanogaster (fruit fly)	Nf1	NP_733132.2
	NF1	XP_002800429.1
Canis lupus familiaris (dog)	NF1	XP_537738.3
Mus musculus (house mouse)	Nf1	NP_035027.1
Gallus gallus (chicken)	NF1	XP_004946843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003140	XP_312829.4
Xenopus tropicalis (tropical clawed frog)	nf1	XP_002936865.2
Pan troglodytes (chimpanzee)	NF1	XP_511395.2

Gene Ontology

The following nf1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nf1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54123	XM_017358082.1 Latest version!	Danio rerio neurofibromin 1b (nf1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa16410	XM_005169189.4 Latest version!	Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa16412	XM_005169190.4 Latest version!	Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa16410	XM_005169189.3	Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa16412	XM_005169190.3	Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nf1b ( XM_017358082.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213571.1 Danio rerio neurofibromin 1b (nf1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa54123
Clone ID Related Accession (Same CDS sequence)	XM_017358082.1
Accession Version	XM_017358082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurofibromin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG AGTTGGTCCA GTGTTAACCA TCACAAAGGT CAAAGTCCTA 
AAGTCAGATG AGGCGATCCG TCCATCAGCT GTGCTTTATT TTCCCCTGCA TTTCTATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213571.1
CDS	283..4965
Translation	MAAQKPVEWVQAVISRFDEQLPVKVGVQNTHTKISTEHNKECLINISRYKFSLVISGLTNILKNVNNMRIFGDTAERNLYLSQLIILDTLEKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQHAAELRSSACGVLFSLSCSNFNAVFSRISTRLQELTVCSEDTVDVHDIELIQYINVDGCKLKRLLQETVFKFKALKKPAQLSVIFNLEKAFWNWVENYPDEFTRLYQRPQTDMADCAEKLFDLVDSFAESTKRKAAVWPLQIILLILCPDITQDMSRDAAEDAKDNKKLFLENLRKALTGHGGSKQLTESAAIACVKLCKASTYISWEDHSVIFLLVQSIVMDLKGLLFNPVKPFSRGAGSQSADLDLMIDCFVSCFRINPHNNQQFKVCLASSSPSTFHFVLVNSLHRIITNSPLDWWPKIDTVYCYSGELRLMFAETLGRVVQGCSHTTLRLTPSLTFKEKMSSLKFKEKTTDLDTRSLKYLLLALVKLIHADPKLMLHNPGKQGLEVHSSTAELITGLVQLVPLANMGEISQEAMEALLVLHQPESIELWNPDAPMETFWDISSQVLYFICKNLFGHPLVSSTEILKWLREILICRNKFLLRNKDRATVGSNIATCRQAQTKLEVCLYMFLWSPDMEAVLVAMSCFRYLCEEAEIRCGVEDIPVQSLLPNYNTFMEFASVSNMMVTGRSALQKRVMALLRKIELPTPGNTEAWEDTHAKWEHATKLILSHPKTKLEDGQEWINMTGFLCALGGVCLQQRHVSALATYSPPMGTLNDRKGSMVSMSSCEGSPETPLGRFLDRLLSLMVCSHEKGHQIRTNVKDLVGLELSPVLYPMLFNKLKNSISRFFDAQGPVPINETNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRILGNMVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDEGQPVVGRKRGMSRRLASLRHCTILAMSNLLSANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALASVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRSVIAGTEWHMLSFEVDPTRLENGENLEENQRNLLRMTERFFKAIISSSGEFPPQLRSVCHCLFQATCHSLLSKASVKDRKEVRKAVVSQRFPQNSIGAVGSAMFLRFVNPAIVSPYEAGILDKKPQPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPASDSLNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLHIFYQAGTSKNGNPVFYYVTRRVGPVLTITKVKVLKSDEAIRPSAVLYFPLHFYL

Target ORF information:

RefSeq Version	XM_017358082.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurofibromin 1b (nf1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358082.1

ORF Insert Sequence:

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG AGTTGGTCCA GTGTTAACCA TCACAAAGGT CAAAGTCCTA 
AAGTCAGATG AGGCGATCCG TCCATCAGCT GTGCTTTATT TTCCCCTGCA TTTCTATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nf1b ( XM_005169189.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169246.1 Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16410
Clone ID Related Accession (Same CDS sequence)	XM_005169189.3 , XM_005169189.4
Accession Version	XM_005169189.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurofibromin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC 
CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA 
ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC 
TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG 
GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG 
TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG 
CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA 
CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC 
TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT 
TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC 
CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC 
CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC 
AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG 
GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC 
CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT 
CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG 
TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT 
TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT 
GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA 
AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT 
CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC 
ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG 
GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC 
ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG 
CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT 
GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG 
GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC 
AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC 
GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC 
TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC 
ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG 
CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA 
CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA 
CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG 
AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT 
GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC 
TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT 
CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT 
GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC 
TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA 
GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG 
TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG 
GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA 
CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT 
TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC 
CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA 
CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG 
CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG 
CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA 
TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG 
TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG 
ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT 
GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT 
CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA 
GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG 
TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG 
CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC 
ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT 
CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT 
TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT 
CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG 
GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169246.1
CDS	279..8558
Translation	MAAQKPVEWVQAVISRFDEQLPVKVGVQNTHTKISTEHNKECLINISRYKFSLVISGLTNILKNVNNMRIFGDTAERNLYLSQLIILDTLEKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQHAAELRSSACGVLFSLSCSNFNAVFSRISTRLQELTVCSEDTVDVHDIELIQYINVDGCKLKRLLQETVFKFKALKKPAQLSVIFNLEKAFWNWVENYPDEFTRLYQRPQTDMADCAEKLFDLVDSFAESTKRKAAVWPLQIILLILCPDITQDMSRDAAEDAKDNKKLFLENLRKALTGHGGSKQLTESAAIACVKLCKASTYISWEDHSVIFLLVQSIVMDLKGLLFNPVKPFSRGAGSQSADLDLMIDCFVSCFRINPHNNQQFKVCLASSSPSTFHFVLVNSLHRIITNSPLDWWPKIDTVYCYSGELRLMFAETLGRVVQGCSHTTLRLTPSLTFKEKMSSLKFKEKTTDLDTRSLKYLLLALVKLIHADPKLMLHNPGKQGLEVHSSTAELITGLVQLVPLANMGEISQEAMEALLVLHQPESIELWNPDAPMETFWDISSQVLYFICKNLFGHPLVSSTEILKWLREILICRNKFLLRNKDRATVGSNIATCRQAQTKLEVCLYMFLWSPDMEAVLVAMSCFRYLCEEAEIRCGVEDIPVQSLLPNYNTFMEFASVSNMMVTGRSALQKRVMALLRKIELPTPGNTEAWEDTHAKWEHATKLILSHPKTKLEDGQEWINMTGFLCALGGVCLQQRHVSALATYSPPMGTLNDRKGSMVSMSSCEGSPETPLGRFLDRLLSLMVCSHEKGHQIRTNVKDLVGLELSPVLYPMLFNKLKNSISRFFDAQGPVPINETNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRILGNMVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDEGQPVVGRKRGMSRRLASLRHCTILAMSNLLSANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALASVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRSVIAGTEWHMLSFEVDPTRLENGENLEENQRNLLRMTERFFKAIISSSGEFPPQLRSVCHCLFQATCHSLLSKASVKDRKEVRKAVVSQRFPQNSIGAVGSAMFLRFVNPAIVSPYEAGILDKKPQPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPASDSLNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLHIFYQAGTSKNGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYELVVDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVAAIYIYNCNTWVREYTKYHERLLTGLKGSKKLLFIDSPARLAEHVEPEQQKLPAATLALEEDLKVFHNALKLAHRDTKVSIKVGSTAVQVTSAERTRVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECESIVHSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISQTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLLNLVRFCKHSDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIIRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLSFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALSSLETVTEALLEIMEACMRDIPGCKWLDQWTDLAQKFAFQYNPSLQPRALVVFGCISKRVTHTQMKQIIRILSKASLLLSIDRGLESCLKGPDNCNSQVLIEATVIALTKLQPLLNKDSAMHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGFRHPSPTTVARTIRILHMLLALVGKHQNCDKFEVNTQSVAYLAALLPVSEEVRSRCSLKHRKLLLFSDVTMENVQMDPYSVHHEDPSGRTLRENELWASPKVSERYLVAHFPIMGQISPRTRKSMSLDMGQPSQVNTKKLLGTRKSFDLLLSDSKAPKRPEIETGITTPPKMRRVAENEYEIETPRISHHHLRKVSVSESNVLLNEEVLTDPKIQALLLTVLATQVKNTSDEFEQRILYEFLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPHLLNPVHGIVQSVVYHEESPPQYQPSYLQTFGFNGLWRFAGPFSKQTQFPDYAELIVKFLDALIDTYLPGIDEETSEEGLRTPTSPYPPPVQSQLSITANLNLSNSMTSLATSQHAPGMDKEKVDLSPTTGLAAGGRTRHGSASQVQKQRSTGSFKRPSIKKIV

Target ORF information:

RefSeq Version	XM_005169189.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169189.3

ORF Insert Sequence:

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC 
CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA 
ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC 
TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG 
GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG 
TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG 
CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA 
CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC 
TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT 
TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC 
CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC 
CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC 
AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG 
GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC 
CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT 
CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG 
TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT 
TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT 
GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA 
AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT 
CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC 
ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG 
GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC 
ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG 
CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT 
GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG 
GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC 
AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC 
GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC 
TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC 
ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG 
CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA 
CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA 
CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG 
AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT 
GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC 
TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT 
CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT 
GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC 
TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA 
GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG 
TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG 
GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA 
CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT 
TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC 
CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA 
CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG 
CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG 
CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA 
TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG 
TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG 
ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT 
GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT 
CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA 
GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG 
TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG 
CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC 
ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT 
CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT 
TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT 
CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG 
GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nf1b ( XM_005169189.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169246.1 Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

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CloneID	ODa16410
Clone ID Related Accession (Same CDS sequence)	XM_005169189.3 , XM_005169189.4
Accession Version	XM_005169189.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurofibromin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169189.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC 
CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA 
ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC 
TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG 
GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG 
TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG 
CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA 
CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC 
TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT 
TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC 
CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC 
CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC 
AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG 
GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC 
CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT 
CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG 
TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT 
TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT 
GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA 
AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT 
CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC 
ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG 
GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC 
ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG 
CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT 
GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG 
GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC 
AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC 
GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC 
TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC 
ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG 
CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA 
CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA 
CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG 
AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT 
GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC 
TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT 
CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT 
GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC 
TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA 
GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG 
TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG 
GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA 
CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT 
TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC 
CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA 
CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG 
CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG 
CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA 
TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG 
TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG 
ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT 
GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT 
CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA 
GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG 
TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG 
CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC 
ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT 
CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT 
TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT 
CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG 
GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169246.1
CDS	265..8544
Translation	MAAQKPVEWVQAVISRFDEQLPVKVGVQNTHTKISTEHNKECLINISRYKFSLVISGLTNILKNVNNMRIFGDTAERNLYLSQLIILDTLEKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQHAAELRSSACGVLFSLSCSNFNAVFSRISTRLQELTVCSEDTVDVHDIELIQYINVDGCKLKRLLQETVFKFKALKKPAQLSVIFNLEKAFWNWVENYPDEFTRLYQRPQTDMADCAEKLFDLVDSFAESTKRKAAVWPLQIILLILCPDITQDMSRDAAEDAKDNKKLFLENLRKALTGHGGSKQLTESAAIACVKLCKASTYISWEDHSVIFLLVQSIVMDLKGLLFNPVKPFSRGAGSQSADLDLMIDCFVSCFRINPHNNQQFKVCLASSSPSTFHFVLVNSLHRIITNSPLDWWPKIDTVYCYSGELRLMFAETLGRVVQGCSHTTLRLTPSLTFKEKMSSLKFKEKTTDLDTRSLKYLLLALVKLIHADPKLMLHNPGKQGLEVHSSTAELITGLVQLVPLANMGEISQEAMEALLVLHQPESIELWNPDAPMETFWDISSQVLYFICKNLFGHPLVSSTEILKWLREILICRNKFLLRNKDRATVGSNIATCRQAQTKLEVCLYMFLWSPDMEAVLVAMSCFRYLCEEAEIRCGVEDIPVQSLLPNYNTFMEFASVSNMMVTGRSALQKRVMALLRKIELPTPGNTEAWEDTHAKWEHATKLILSHPKTKLEDGQEWINMTGFLCALGGVCLQQRHVSALATYSPPMGTLNDRKGSMVSMSSCEGSPETPLGRFLDRLLSLMVCSHEKGHQIRTNVKDLVGLELSPVLYPMLFNKLKNSISRFFDAQGPVPINETNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRILGNMVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDEGQPVVGRKRGMSRRLASLRHCTILAMSNLLSANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALASVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRSVIAGTEWHMLSFEVDPTRLENGENLEENQRNLLRMTERFFKAIISSSGEFPPQLRSVCHCLFQATCHSLLSKASVKDRKEVRKAVVSQRFPQNSIGAVGSAMFLRFVNPAIVSPYEAGILDKKPQPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPASDSLNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLHIFYQAGTSKNGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYELVVDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVAAIYIYNCNTWVREYTKYHERLLTGLKGSKKLLFIDSPARLAEHVEPEQQKLPAATLALEEDLKVFHNALKLAHRDTKVSIKVGSTAVQVTSAERTRVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECESIVHSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISQTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLLNLVRFCKHSDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIIRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLSFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALSSLETVTEALLEIMEACMRDIPGCKWLDQWTDLAQKFAFQYNPSLQPRALVVFGCISKRVTHTQMKQIIRILSKASLLLSIDRGLESCLKGPDNCNSQVLIEATVIALTKLQPLLNKDSAMHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGFRHPSPTTVARTIRILHMLLALVGKHQNCDKFEVNTQSVAYLAALLPVSEEVRSRCSLKHRKLLLFSDVTMENVQMDPYSVHHEDPSGRTLRENELWASPKVSERYLVAHFPIMGQISPRTRKSMSLDMGQPSQVNTKKLLGTRKSFDLLLSDSKAPKRPEIETGITTPPKMRRVAENEYEIETPRISHHHLRKVSVSESNVLLNEEVLTDPKIQALLLTVLATQVKNTSDEFEQRILYEFLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPHLLNPVHGIVQSVVYHEESPPQYQPSYLQTFGFNGLWRFAGPFSKQTQFPDYAELIVKFLDALIDTYLPGIDEETSEEGLRTPTSPYPPPVQSQLSITANLNLSNSMTSLATSQHAPGMDKEKVDLSPTTGLAAGGRTRHGSASQVQKQRSTGSFKRPSIKKIV

Target ORF information:

RefSeq Version	XM_005169189.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurofibromin 1b (nf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169189.4

ORF Insert Sequence:

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGCTAC ATGTCACTCT CTCCTGTCTA AAGCCTCTGT CAAAGACAGA 
AAGGAAGTGA GGAAAGCAGT CGTGAGCCAG CGTTTTCCTC AGAACAGCAT TGGAGCTGTA 
GGCAGTGCCA TGTTCTTGCG ATTCGTGAAT CCAGCTATAG TCTCTCCATA CGAGGCTGGG 
ATTCTTGACA AAAAACCTCA GCCTAGGATA GAAAGAGGCC TAAAACTCAT GTCCAAGATT 
CTCCAAAGTA TTGCCAACCA TGTTTTGTTC ACAAAAGAGG AGCACATGAG GCCTTTCAAT 
GACTTTGTGA AGAGTAATTT TGATGCTGCC AGAAGATTTT TCCTGGACAT CGCCTCTGAT 
TGCCCTGCTA GCGACTCTCT CAACCATAGC CTGTCCTTTA TTAGTGATGG GAACGTTCTG 
GCTCTGCACA GACTGCTGTG GAACAATCAA GAAAAAATTG GCCAGTATTT ATCTAGCAAC 
AGGGATCATA AAGCAGTCGG AAGAAGGCCT TTTGATAAGA TGGCCACCCT GCTCGCTTAC 
CTGGGTCCCC CAGAACACAA ACCTGTGGCA GATACTCACT GGTCCAGTTT GAATCTTACC 
AGCTCCAAAT TTGAGGAGTT CATGACAAGG CACCAGGTCC ATGAGAAAGA AGAGTTCAAA 
GCATTGAAGA CTCTACACAT CTTCTACCAG GCTGGAACAT CAAAGAACGG AAACCCTGTG 
TTTTACTATG TGACGCGCAG GTTCAAGACG GGCCAGATAA ATGGGGACTT GCTTATATAC 
CATGTCCTTC TCACCCTCAA ACCATACTAT GCCAAACCCT ATGAGCTAGT GGTGGATTTA 
ACCCACGCTG GTCCAAGCAA CCGCTTCAAG ACCGACTTCC TCTCCAAGTG GTTTGTCGTC 
TTTCCGGGAT TTGCATATGA AAACGTTGCT GCCATTTACA TCTATAACTG TAACACATGG 
GTCCGGGAAT ATACCAAATA TCATGAGAGA CTCTTGACGG GACTCAAAGG CAGTAAGAAG 
TTACTGTTCA TTGATTCGCC TGCACGGTTG GCTGAACATG TGGAACCAGA GCAACAGAAG 
CTTCCGGCAG CTACACTTGC ACTGGAAGAA GACCTGAAAG TCTTTCACAA TGCGCTAAAA 
CTGGCCCACA GAGACACCAA AGTATCTATT AAGGTGGGCA GCACAGCAGT GCAGGTCACC 
TCAGCCGAAC GAACACGAGT TTTGGGTCAA TCGGTCTTCC TCAACGACAT CTACTATGCT 
TCAGAGATCG AGGAGATTTG CCTAGTGGAC GAAAATCAAT TCACACTGAC TATCGCTAAC 
CAAGGGACGC CGCTGACCTT CATGCACCAG GAGTGTGAGA GCATAGTTCA CTCCATTATC 
CACATCCGCA CGCGCTGGGA GCTTTCCCAA CCTGACTCCA TTCCTCAACA CACCAAAATC 
AGGCCCAAAG ATGTTCCTGG GACCCTTCTC AACATCGCTC TCCTAAACCT GGGCAGCTCG 
GACCCCAGTT TAAGGTCTGC AGCTTATAAC CTCCTGTGCG CCTTAACATG CACCTTTAAC 
CTGAAGATCG AAGGGCAGCT TCTGGAGACG TCTGGCCTGT GCATCCCTGC CAATAACACT 
CTATTCATCG TGTCCATTAG CCAGACTCTA GCGGCTAATG AGCCTCACCT GACCCTGGAG 
TTCCTGGAGG AGTGTATCTC TGGATTCAGC AAGTCCAGTA TAGAACTAAA GCACCTGTGT 
TTAGAGTACA TGACTCCTTG GCTGCTGAAC CTGGTACGCT TCTGCAAGCA TAGCGATGAT 
GCCAAAAGGC AACGAGTCAC CGCCATTCTG GACAAACTCA TTACCATGAC CATCAACGAA 
AAGCAGATGT ATCCATCCAT TCAGGCCAAG ATCTGGGGGA GCTTGGGCCA GATAACTGAT 
CTCCTTGATG TAGTTCTGGA CAGTTTCATT AAAACCAGTG CGACAGGAGG TCTCGGATCC 
ATCAAAGCTG AAGTGATGGC GGACACTGCA GTGGCTTTGG CTTCTGGGAA TGTCAAATTG 
GTGTCAAGCA AGGTGATCAT TCGCATGTGC AAAATCATCG ATAAGACGTG TCTGTCGCCC 
ACACCAACAC TGGAGCAGCA CCTGATGTGG GATGATATCG CCATCCTGGC CCGGTACATG 
CTCATGCTGT CCTTCAACAA CTCTCTGGAT GTAGCCGCAC ACCTGCCGTA CCTGTTTCAT 
GTGGTGACCC TGCTGGTGGC CACGGGGCCT CTGTCTCTGC GGGCCTCCAC GCATGGCTTG 
GTCATCAACA TCATCCACTC CCTCTGCACA TGCTCCCAGC TGAGCTTCAG CGAGGAGACC 
AAGCAGGTGT TGCGTCTCAG TCTGACCGAG TTCTCCCTGC CGAAGTTCTA CCTGCTGTTC 
GGCATCAGCA AGGTCAAGTC GGCTGCGGTC ATTGCATTTC GTTCCAGCTA CAGGGATCGC 
TCATTCTCGC CGGGGTCCTA TGAGAGAGAG ACCTTTGCTC TGTCATCTCT GGAGACGGTC 
ACAGAGGCGC TGCTGGAGAT CATGGAGGCT TGCATGAGGG ACATTCCAGG CTGTAAATGG 
CTAGACCAGT GGACCGATCT GGCGCAGAAG TTTGCATTTC AATATAATCC ATCCCTCCAA 
CCACGGGCAC TTGTGGTATT CGGCTGCATC AGCAAACGAG TCACACATAC GCAGATGAAA 
CAGATCATAC GGATCTTGAG CAAGGCCAGC CTTCTCCTCA GCATCGACCG GGGTTTGGAG 
AGCTGCCTGA AGGGTCCCGA TAACTGTAAC AGCCAGGTGC TAATCGAAGC CACAGTCATT 
GCTCTCACTA AACTACAACC TTTGCTCAAC AAGGACTCAG CGATGCACAA AGCTCTGTTC 
TGGGTCGCTG TGGCCGTACT GCAGTTGGAT GAGGTGAATC TGTATTCTGC AGGAACTGCT 
CTGCTTGAAC AGAACCTACA CACACTCGAT AGCCTGAGAG TCTTCAACGA CAAGAGTCCT 
GAGGAGGTGT TTATGGAGAT CCGTCAGCCT CTGGAATGGC ACTGTAAACA AATGGACCAC 
TTTGTTGGCC TCAATTTTAA CTCCAACTTC AATTTTGCTT TGGTCGGTCA CCTGTTGAAA 
GGGTTCAGAC ACCCCTCCCC CACCACAGTG GCACGTACCA TCCGCATCCT GCACATGCTG 
TTGGCGTTAG TAGGAAAACA CCAGAACTGT GATAAATTTG AGGTGAACAC ACAGAGTGTG 
GCGTATCTGG CAGCGCTTCT CCCGGTGTCA GAGGAAGTGC GCAGCCGCTG CAGTCTGAAA 
CACAGAAAAT TGTTGCTTTT CTCTGATGTC ACAATGGAGA ATGTTCAAAT GGATCCGTAT 
TCTGTACATC ACGAGGATCC AAGCGGCAGA ACGTTGCGGG AAAACGAGCT GTGGGCATCC 
CCGAAAGTCT CAGAGCGATA CTTGGTGGCC CATTTCCCCA TAATGGGGCA GATCAGCCCA 
CGAACACGCA AATCAATGAG CTTGGACATG GGACAACCCT CTCAAGTCAA CACCAAGAAG 
CTTCTGGGCA CCAGAAAAAG CTTTGACCTC CTGTTATCAG ACAGCAAAGC ACCAAAGAGG 
CCTGAAATAG AGACTGGTAT CACAACGCCA CCCAAGATGA GGAGAGTGGC TGAAAACGAA 
TATGAAATCG AAACCCCAAG AATCTCCCAT CACCACTTGC GGAAGGTTTC TGTGTCCGAG 
TCCAATGTCC TGCTTAATGA AGAAGTTCTC ACAGATCCCA AAATCCAAGC ACTGCTTCTG 
ACTGTGCTGG CCACTCAGGT GAAAAACACT TCAGATGAAT TTGAGCAGAG GATCTTGTAT 
GAGTTTCTAG CCGAGGCCAG TGTTGTCTTC CCAAAAGTCT TTCCTGTTGT TCATAATCTT 
CTGGACTCTA AGATTAATAC GTTGTTGTCG CTGTGTCAGG ACCCACATCT TCTGAATCCA 
GTTCATGGAA TTGTTCAGAG TGTAGTTTAT CATGAAGAGT CTCCACCTCA GTACCAACCG 
TCCTACCTGC AAACTTTTGG GTTTAATGGA CTCTGGAGGT TTGCCGGTCC TTTTTCGAAG 
CAAACTCAGT TCCCAGACTA TGCAGAGCTG ATAGTCAAGT TTCTGGATGC ACTGATTGAC 
ACCTATCTTC CGGGCATTGA TGAGGAAACA AGCGAGGAGG GTCTGCGGAC ACCCACCTCT 
CCATACCCTC CACCTGTGCA GAGCCAGCTC AGCATCACTG CCAACCTCAA CCTCTCAAAT 
TCAATGACCT CGCTGGCCAC CTCCCAACAT GCACCAGGAA TGGATAAAGA GAAAGTGGAT 
CTATCCCCCA CCACGGGTTT GGCAGCCGGC GGACGCACAC GCCACGGCTC GGCCAGCCAG 
GTCCAGAAAC AGCGCAGCAC CGGCAGCTTT AAGAGACCTA GCATTAAAAA GATTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nf1b ( XM_005169190.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169247.1 Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

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CloneID	ODa16412
Clone ID Related Accession (Same CDS sequence)	XM_005169190.3 , XM_005169190.4
Accession Version	XM_005169190.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurofibromin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC 
AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT 
CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC 
CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC 
TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC 
CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT 
CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG 
GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG 
GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC 
TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA 
TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT 
TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT 
GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC 
CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT 
CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC 
CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA 
CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT 
CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG 
GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA 
GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA 
GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA 
GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC 
ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG 
CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC 
CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG 
AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA 
TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC 
CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA 
GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC 
AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG 
CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC 
CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC 
AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG 
TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA 
CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC 
ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG 
GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC 
ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG 
CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC 
ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA 
TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA 
GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA 
GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA 
CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG 
ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC 
TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT 
CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG 
GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG 
CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG 
GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT 
GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG 
TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG 
GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG 
TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC 
AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT 
GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG 
AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA 
ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT 
CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT 
GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT 
GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC 
AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT 
GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG 
TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG 
GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT 
CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC 
TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA 
ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC 
TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA 
TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA 
ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA 
TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC 
CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169247.1
CDS	279..8495
Translation	MAAQKPVEWVQAVISRFDEQLPVKVGVQNTHTKISTEHNKECLINISRYKFSLVISGLTNILKNVNNMRIFGDTAERNLYLSQLIILDTLEKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQHAAELRSSACGVLFSLSCSNFNAVFSRISTRLQELTVCSEDTVDVHDIELIQYINVDGCKLKRLLQETVFKFKALKKPAQLSVIFNLEKAFWNWVENYPDEFTRLYQRPQTDMADCAEKLFDLVDSFAESTKRKAAVWPLQIILLILCPDITQDMSRDAAEDAKDNKKLFLENLRKALTGHGGSKQLTESAAIACVKLCKASTYISWEDHSVIFLLVQSIVMDLKGLLFNPVKPFSRGAGSQSADLDLMIDCFVSCFRINPHNNQQFKVCLASSSPSTFHFVLVNSLHRIITNSPLDWWPKIDTVYCYSGELRLMFAETLGRVVQGCSHTTLRLTPSLTFKEKMSSLKFKEKTTDLDTRSLKYLLLALVKLIHADPKLMLHNPGKQGLEVHSSTAELITGLVQLVPLANMGEISQEAMEALLVLHQPESIELWNPDAPMETFWDISSQVLYFICKNLFGHPLVSSTEILKWLREILICRNKFLLRNKDRATVGSNIATCRQAQTKLEVCLYMFLWSPDMEAVLVAMSCFRYLCEEAEIRCGVEDIPVQSLLPNYNTFMEFASVSNMMVTGRSALQKRVMALLRKIELPTPGNTEAWEDTHAKWEHATKLILSHPKTKLEDGQEWINMTGFLCALGGVCLQQRHVSALATYSPPMGTLNDRKGSMVSMSSCEGSPETPLGRFLDRLLSLMVCSHEKGHQIRTNVKDLVGLELSPVLYPMLFNKLKNSISRFFDAQGPVPINETNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRILGNMVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDEGQPVVGRKRGMSRRLASLRHCTILAMSNLLSANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALASVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRSVIAGTEWHMLSFEVDPTRLENGENLEENQRNLLRMTERFFKAIISSSGEFPPQLRSVCHCLFQVVSQRFPQNSIGAVGSAMFLRFVNPAIVSPYEAGILDKKPQPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPASDSLNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLHIFYQAGTSKNGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYELVVDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVAAIYIYNCNTWVREYTKYHERLLTGLKGSKKLLFIDSPARLAEHVEPEQQKLPAATLALEEDLKVFHNALKLAHRDTKVSIKVGSTAVQVTSAERTRVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECESIVHSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISQTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLLNLVRFCKHSDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIIRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLSFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALSSLETVTEALLEIMEACMRDIPGCKWLDQWTDLAQKFAFQYNPSLQPRALVVFGCISKRVTHTQMKQIIRILSKASLLLSIDRGLESCLKGPDNCNSQVLIEATVIALTKLQPLLNKDSAMHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGFRHPSPTTVARTIRILHMLLALVGKHQNCDKFEVNTQSVAYLAALLPVSEEVRSRCSLKHRKLLLFSDVTMENVQMDPYSVHHEDPSGRTLRENELWASPKVSERYLVAHFPIMGQISPRTRKSMSLDMGQPSQVNTKKLLGTRKSFDLLLSDSKAPKRPEIETGITTPPKMRRVAENEYEIETPRISHHHLRKVSVSESNVLLNEEVLTDPKIQALLLTVLATQVKNTSDEFEQRILYEFLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPHLLNPVHGIVQSVVYHEESPPQYQPSYLQTFGFNGLWRFAGPFSKQTQFPDYAELIVKFLDALIDTYLPGIDEETSEEGLRTPTSPYPPPVQSQLSITANLNLSNSMTSLATSQHAPGMDKEKVDLSPTTGLAAGGRTRHGSASQVQKQRSTGSFKRPSIKKIV

Target ORF information:

RefSeq Version	XM_005169190.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169190.3

ORF Insert Sequence:

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC 
AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT 
CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC 
CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC 
TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC 
CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT 
CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG 
GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG 
GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC 
TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA 
TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT 
TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT 
GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC 
CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT 
CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC 
CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA 
CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT 
CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG 
GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA 
GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA 
GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA 
GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC 
ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG 
CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC 
CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG 
AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA 
TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC 
CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA 
GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC 
AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG 
CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC 
CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC 
AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG 
TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA 
CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC 
ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG 
GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC 
ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG 
CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC 
ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA 
TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA 
GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA 
GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA 
CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG 
ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC 
TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT 
CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG 
GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG 
CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG 
GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT 
GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG 
TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG 
GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG 
TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC 
AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT 
GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG 
AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA 
ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT 
CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT 
GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT 
GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC 
AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT 
GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG 
TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG 
GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT 
CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC 
TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA 
ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC 
TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA 
TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA 
ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA 
TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC 
CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nf1b ( XM_005169190.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169247.1 Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

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CloneID	ODa16412
Clone ID Related Accession (Same CDS sequence)	XM_005169190.3 , XM_005169190.4
Accession Version	XM_005169190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurofibromin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC 
AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT 
CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC 
CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC 
TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC 
CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT 
CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG 
GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG 
GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC 
TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA 
TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT 
TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT 
GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC 
CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT 
CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC 
CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA 
CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT 
CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG 
GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA 
GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA 
GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA 
GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC 
ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG 
CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC 
CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG 
AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA 
TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC 
CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA 
GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC 
AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG 
CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC 
CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC 
AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG 
TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA 
CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC 
ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG 
GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC 
ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG 
CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC 
ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA 
TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA 
GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA 
GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA 
CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG 
ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC 
TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT 
CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG 
GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG 
CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG 
GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT 
GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG 
TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG 
GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG 
TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC 
AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT 
GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG 
AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA 
ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT 
CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT 
GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT 
GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC 
AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT 
GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG 
TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG 
GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT 
CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC 
TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA 
ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC 
TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA 
TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA 
ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA 
TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC 
CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169247.1
CDS	265..8481
Translation	MAAQKPVEWVQAVISRFDEQLPVKVGVQNTHTKISTEHNKECLINISRYKFSLVISGLTNILKNVNNMRIFGDTAERNLYLSQLIILDTLEKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQHAAELRSSACGVLFSLSCSNFNAVFSRISTRLQELTVCSEDTVDVHDIELIQYINVDGCKLKRLLQETVFKFKALKKPAQLSVIFNLEKAFWNWVENYPDEFTRLYQRPQTDMADCAEKLFDLVDSFAESTKRKAAVWPLQIILLILCPDITQDMSRDAAEDAKDNKKLFLENLRKALTGHGGSKQLTESAAIACVKLCKASTYISWEDHSVIFLLVQSIVMDLKGLLFNPVKPFSRGAGSQSADLDLMIDCFVSCFRINPHNNQQFKVCLASSSPSTFHFVLVNSLHRIITNSPLDWWPKIDTVYCYSGELRLMFAETLGRVVQGCSHTTLRLTPSLTFKEKMSSLKFKEKTTDLDTRSLKYLLLALVKLIHADPKLMLHNPGKQGLEVHSSTAELITGLVQLVPLANMGEISQEAMEALLVLHQPESIELWNPDAPMETFWDISSQVLYFICKNLFGHPLVSSTEILKWLREILICRNKFLLRNKDRATVGSNIATCRQAQTKLEVCLYMFLWSPDMEAVLVAMSCFRYLCEEAEIRCGVEDIPVQSLLPNYNTFMEFASVSNMMVTGRSALQKRVMALLRKIELPTPGNTEAWEDTHAKWEHATKLILSHPKTKLEDGQEWINMTGFLCALGGVCLQQRHVSALATYSPPMGTLNDRKGSMVSMSSCEGSPETPLGRFLDRLLSLMVCSHEKGHQIRTNVKDLVGLELSPVLYPMLFNKLKNSISRFFDAQGPVPINETNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRILGNMVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDEGQPVVGRKRGMSRRLASLRHCTILAMSNLLSANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETALADRFERLVELVTMMGDQGELPIAMALASVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRSVIAGTEWHMLSFEVDPTRLENGENLEENQRNLLRMTERFFKAIISSSGEFPPQLRSVCHCLFQVVSQRFPQNSIGAVGSAMFLRFVNPAIVSPYEAGILDKKPQPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPASDSLNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLHIFYQAGTSKNGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYELVVDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVAAIYIYNCNTWVREYTKYHERLLTGLKGSKKLLFIDSPARLAEHVEPEQQKLPAATLALEEDLKVFHNALKLAHRDTKVSIKVGSTAVQVTSAERTRVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECESIVHSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISQTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLLNLVRFCKHSDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIIRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLSFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALSSLETVTEALLEIMEACMRDIPGCKWLDQWTDLAQKFAFQYNPSLQPRALVVFGCISKRVTHTQMKQIIRILSKASLLLSIDRGLESCLKGPDNCNSQVLIEATVIALTKLQPLLNKDSAMHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGFRHPSPTTVARTIRILHMLLALVGKHQNCDKFEVNTQSVAYLAALLPVSEEVRSRCSLKHRKLLLFSDVTMENVQMDPYSVHHEDPSGRTLRENELWASPKVSERYLVAHFPIMGQISPRTRKSMSLDMGQPSQVNTKKLLGTRKSFDLLLSDSKAPKRPEIETGITTPPKMRRVAENEYEIETPRISHHHLRKVSVSESNVLLNEEVLTDPKIQALLLTVLATQVKNTSDEFEQRILYEFLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPHLLNPVHGIVQSVVYHEESPPQYQPSYLQTFGFNGLWRFAGPFSKQTQFPDYAELIVKFLDALIDTYLPGIDEETSEEGLRTPTSPYPPPVQSQLSITANLNLSNSMTSLATSQHAPGMDKEKVDLSPTTGLAAGGRTRHGSASQVQKQRSTGSFKRPSIKKIV

Target ORF information:

RefSeq Version	XM_005169190.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurofibromin 1b (nf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169190.4

ORF Insert Sequence:

ATGGCGGCGC AGAAGCCCGT GGAGTGGGTC CAGGCCGTCA TCAGCCGATT CGACGAGCAG 
CTTCCTGTGA AAGTCGGTGT TCAAAACACA CACACTAAAA TCAGCACAGA ACACAATAAG 
GAATGCCTGA TCAACATCTC CAGGTACAAA TTCTCCCTGG TCATCAGCGG ACTCACCAAC 
ATACTGAAGA ACGTCAACAA CATGAGAATA TTTGGAGATA CTGCAGAGAG GAATCTTTAC 
CTGTCACAGC TAATCATCTT GGACACATTG GAGAAGTGCT TGGCAGGGCA ACCGAAAGAC 
AGCATGCGTC TAGACGAGAC GATGCTTGTG AAGCAGCTGT TGCCGGAGAT CTGTCATTTC 
ATCCACACGT ATCGTGAGGG AAACCAGCAC GCGGCTGAGC TGAGGAGCTC CGCCTGTGGT 
GTCCTGTTCT CTCTGAGCTG CAGTAACTTC AACGCCGTCT TCAGCCGCAT CTCCACCAGG 
CTACAGGAGC TGACTGTCTG CTCGGAAGAT ACTGTGGACG TGCACGACAT TGAGCTCATT 
CAGTACATCA ATGTGGATGG CTGTAAACTC AAACGCCTCC TCCAAGAAAC TGTCTTTAAA 
TTTAAAGCCT TAAAGAAGCC GGCACAGTTA AGTGTCATCT TCAATTTAGA GAAGGCGTTT 
TGGAACTGGG TGGAGAATTA TCCTGATGAA TTCACCAGGC TGTACCAGAG GCCTCAAACA 
GACATGGCTG ACTGTGCTGA AAAGCTGTTT GATCTGGTGG ACAGTTTTGC CGAAAGCACG 
AAGAGAAAAG CAGCTGTTTG GCCTCTCCAG ATCATCCTCC TCATCCTCTG TCCTGATATC 
ACGCAAGACA TGTCCAGAGA TGCAGCAGAG GACGCCAAAG ACAACAAGAA GCTGTTTTTG 
GAGAATCTGC GGAAAGCTTT GACTGGCCAC GGTGGAAGCA AACAGCTGAC AGAGAGCGCA 
GCCATCGCCT GCGTTAAACT GTGTAAAGCG TCCACATACA TCAGCTGGGA GGATCACTCT 
GTTATCTTCC TGCTGGTGCA GTCTATTGTC ATGGACCTGA AGGGTTTGCT GTTTAACCCA 
GTTAAGCCGT TTTCCAGAGG CGCAGGAAGT CAGAGTGCAG ACCTGGACTT AATGATCGAC 
TGCTTCGTCT CCTGTTTCAG AATCAACCCA CACAACAATC AGCAGTTTAA AGTGTGTTTG 
GCCTCGTCCT CCCCTTCAAC CTTTCATTTC GTGCTGGTGA ATTCTCTCCA CCGCATCATC 
ACTAATTCTC CACTGGACTG GTGGCCGAAG ATCGACACAG TGTACTGTTA TTCTGGAGAG 
CTACGGCTCA TGTTTGCGGA GACTCTGGGA CGAGTGGTGC AGGGCTGCAG TCACACAACG 
CTTCGATTGA CTCCGAGTTT GACGTTTAAG GAGAAAATGA GCAGTCTGAA GTTTAAAGAG 
AAAACCACTG ATCTGGACAC CAGGAGTCTG AAGTATCTGC TCTTGGCTCT GGTGAAACTC 
ATACATGCGG ATCCCAAACT GATGCTCCAT AATCCAGGAA AACAAGGTCT GGAGGTTCAC 
AGCAGCACAG CAGAGCTCAT CACAGGCCTC GTCCAGCTCG TGCCTCTTGC TAACATGGGA 
GAGATTTCCC AGGAGGCCAT GGAGGCTCTG CTGGTCCTGC ATCAGCCTGA AAGCATTGAG 
CTGTGGAACC CGGATGCCCC TATGGAGACC TTCTGGGACA TCAGTTCTCA GGTGCTTTAC 
TTCATCTGTA AGAATCTGTT TGGTCATCCG CTGGTTAGCA GTACTGAGAT TCTGAAATGG 
CTGAGAGAGA TTCTCATCTG CAGAAACAAG TTTCTTCTAA GGAATAAGGA TCGGGCCACA 
GTGGGCAGCA ACATTGCTAC CTGCCGGCAG GCTCAGACCA AGCTGGAGGT CTGTCTCTAC 
ATGTTCCTGT GGAGTCCAGA CATGGAGGCC GTTCTAGTGG CCATGTCCTG TTTCCGTTAC 
CTGTGTGAGG AGGCAGAAAT CCGGTGCGGT GTTGAAGACA TCCCAGTGCA GTCTCTTCTG 
CCAAACTACA ACACCTTCAT GGAGTTTGCC TCTGTCAGTA ACATGATGGT CACAGGTCGT 
TCAGCTCTAC AGAAGAGAGT AATGGCTTTG CTTCGGAAGA TTGAACTTCC CACTCCTGGA 
AACACAGAGG CCTGGGAAGA CACGCATGCC AAATGGGAAC ATGCAACCAA GTTGATTTTA 
AGTCACCCGA AAACGAAACT GGAAGACGGT CAGGAATGGA TCAATATGAC AGGCTTCTTG 
TGTGCGTTGG GCGGTGTGTG TTTGCAGCAG AGGCATGTGT CAGCTCTGGC CACCTACAGT 
CCTCCTATGG GCACCCTGAA TGACCGCAAG GGCTCGATGG TGTCGATGAG CTCTTGTGAG 
GGCAGCCCAG AGACACCTCT CGGCCGCTTT CTGGACCGTC TTCTGTCCCT GATGGTGTGC 
AGTCACGAAA AAGGCCATCA GATCCGCACT AATGTCAAAG ACCTGGTGGG GCTGGAGCTC 
AGTCCAGTGC TTTACCCAAT GCTCTTCAAC AAACTCAAGA ACAGCATCAG CAGATTCTTT 
GATGCCCAGG GGCCGGTGCC AATTAATGAG ACCAACACAC AGTTTGTGGA GCAGACCATC 
GCCATCATGA AGAACCTTCT GGACAACCAT ACAGAAGGTA GCTCGGAGCA TCTGGGTCAA 
GCCAGCATTG AGACCATGAT GCTCAACCTG GTCAGGTATG TGCGTATTCT TGGCAACATG 
GTTCATGCCA TTCAGATTAA AACCAAATTG TGTCAGCTGG TGGAGGTCAT GATGGAGCGC 
AGAGATGATC TTTCCTTCTG CCAGGAGATG AAGTTCAGGA ATAAGATGGT CGAGTATCTG 
ACAGACTGGG TGATGGGAAC CTCCAACCAG GCTGCTGATG ATGATGTCAA ATGCCTCACC 
AGGGATCTGG ATCAGGCGAG TATGGAAGCG GTGGTGTCTC TTCTGGCCGG TTTGCCTCTT 
CAGCCAGAGG AAGGTGACGG AGTGGAGCTG ATGGAGGCCA AATCTCAGCT CTTCCTGAAA 
TACTTCACTC TGTTCATGAA CCTGCTGAAC GACTGCAGCG AGGTGGAGGA CGAGGGTCAG 
CCGGTGGTCG GGAGGAAGCG AGGCATGTCC AGGCGATTGG CATCATTACG ACACTGCACC 
ATCCTGGCCA TGTCCAACCT GCTTAGTGCC AATGTGGACA GCGGCCTGAT GCACTCTATC 
GGCCTGGGCT ATCATAAAGA CCTTCAGACC AGAGCCACCT TTATGGAGGT GCTCACCAAG 
ATTCTTCAGC AAGGCACAGA GTTCGACACG CTGGCAGAGA CGGCTCTGGC GGACCGCTTC 
GAGAGGCTGG TGGAGCTCGT CACCATGATG GGGGATCAGG GCGAGTTGCC CATCGCAATG 
GCTCTGGCCA GTGTGGTGCC CTGTTCACAG TGGGATGAAC TGGCACGTGT TCTGGTAACT 
CTCTTCGACT CGAGGCATCT GTTATATCAG CTCCTGTGGA ATATGTTCTC TAAAGAGGTG 
GAGTTGGCGG ATTCCATGCA AACGTTGTTC CGTGGCAACA GCCTGGCCAG TAAAATCATG 
ACGTTCTGCT TTAAGGTGTA TGGAGCCACA TATCTGCAAA AGCTGCTGGA GCCGCTGTTG 
AGAAGTGTGA TTGCGGGCAC CGAGTGGCAC ATGCTGAGCT TTGAGGTGGA TCCTACCAGA 
CTTGAAAATG GTGAAAATTT GGAGGAGAAC CAGCGGAATC TGCTTCGAAT GACTGAACGC 
TTTTTTAAGG CCATAATCAG TTCATCTGGA GAGTTTCCTC CTCAGCTGCG CAGCGTCTGT 
CACTGTCTCT TTCAGGTCGT GAGCCAGCGT TTTCCTCAGA ACAGCATTGG AGCTGTAGGC 
AGTGCCATGT TCTTGCGATT CGTGAATCCA GCTATAGTCT CTCCATACGA GGCTGGGATT 
CTTGACAAAA AACCTCAGCC TAGGATAGAA AGAGGCCTAA AACTCATGTC CAAGATTCTC 
CAAAGTATTG CCAACCATGT TTTGTTCACA AAAGAGGAGC ACATGAGGCC TTTCAATGAC 
TTTGTGAAGA GTAATTTTGA TGCTGCCAGA AGATTTTTCC TGGACATCGC CTCTGATTGC 
CCTGCTAGCG ACTCTCTCAA CCATAGCCTG TCCTTTATTA GTGATGGGAA CGTTCTGGCT 
CTGCACAGAC TGCTGTGGAA CAATCAAGAA AAAATTGGCC AGTATTTATC TAGCAACAGG 
GATCATAAAG CAGTCGGAAG AAGGCCTTTT GATAAGATGG CCACCCTGCT CGCTTACCTG 
GGTCCCCCAG AACACAAACC TGTGGCAGAT ACTCACTGGT CCAGTTTGAA TCTTACCAGC 
TCCAAATTTG AGGAGTTCAT GACAAGGCAC CAGGTCCATG AGAAAGAAGA GTTCAAAGCA 
TTGAAGACTC TACACATCTT CTACCAGGCT GGAACATCAA AGAACGGAAA CCCTGTGTTT 
TACTATGTGA CGCGCAGGTT CAAGACGGGC CAGATAAATG GGGACTTGCT TATATACCAT 
GTCCTTCTCA CCCTCAAACC ATACTATGCC AAACCCTATG AGCTAGTGGT GGATTTAACC 
CACGCTGGTC CAAGCAACCG CTTCAAGACC GACTTCCTCT CCAAGTGGTT TGTCGTCTTT 
CCGGGATTTG CATATGAAAA CGTTGCTGCC ATTTACATCT ATAACTGTAA CACATGGGTC 
CGGGAATATA CCAAATATCA TGAGAGACTC TTGACGGGAC TCAAAGGCAG TAAGAAGTTA 
CTGTTCATTG ATTCGCCTGC ACGGTTGGCT GAACATGTGG AACCAGAGCA ACAGAAGCTT 
CCGGCAGCTA CACTTGCACT GGAAGAAGAC CTGAAAGTCT TTCACAATGC GCTAAAACTG 
GCCCACAGAG ACACCAAAGT ATCTATTAAG GTGGGCAGCA CAGCAGTGCA GGTCACCTCA 
GCCGAACGAA CACGAGTTTT GGGTCAATCG GTCTTCCTCA ACGACATCTA CTATGCTTCA 
GAGATCGAGG AGATTTGCCT AGTGGACGAA AATCAATTCA CACTGACTAT CGCTAACCAA 
GGGACGCCGC TGACCTTCAT GCACCAGGAG TGTGAGAGCA TAGTTCACTC CATTATCCAC 
ATCCGCACGC GCTGGGAGCT TTCCCAACCT GACTCCATTC CTCAACACAC CAAAATCAGG 
CCCAAAGATG TTCCTGGGAC CCTTCTCAAC ATCGCTCTCC TAAACCTGGG CAGCTCGGAC 
CCCAGTTTAA GGTCTGCAGC TTATAACCTC CTGTGCGCCT TAACATGCAC CTTTAACCTG 
AAGATCGAAG GGCAGCTTCT GGAGACGTCT GGCCTGTGCA TCCCTGCCAA TAACACTCTA 
TTCATCGTGT CCATTAGCCA GACTCTAGCG GCTAATGAGC CTCACCTGAC CCTGGAGTTC 
CTGGAGGAGT GTATCTCTGG ATTCAGCAAG TCCAGTATAG AACTAAAGCA CCTGTGTTTA 
GAGTACATGA CTCCTTGGCT GCTGAACCTG GTACGCTTCT GCAAGCATAG CGATGATGCC 
AAAAGGCAAC GAGTCACCGC CATTCTGGAC AAACTCATTA CCATGACCAT CAACGAAAAG 
CAGATGTATC CATCCATTCA GGCCAAGATC TGGGGGAGCT TGGGCCAGAT AACTGATCTC 
CTTGATGTAG TTCTGGACAG TTTCATTAAA ACCAGTGCGA CAGGAGGTCT CGGATCCATC 
AAAGCTGAAG TGATGGCGGA CACTGCAGTG GCTTTGGCTT CTGGGAATGT CAAATTGGTG 
TCAAGCAAGG TGATCATTCG CATGTGCAAA ATCATCGATA AGACGTGTCT GTCGCCCACA 
CCAACACTGG AGCAGCACCT GATGTGGGAT GATATCGCCA TCCTGGCCCG GTACATGCTC 
ATGCTGTCCT TCAACAACTC TCTGGATGTA GCCGCACACC TGCCGTACCT GTTTCATGTG 
GTGACCCTGC TGGTGGCCAC GGGGCCTCTG TCTCTGCGGG CCTCCACGCA TGGCTTGGTC 
ATCAACATCA TCCACTCCCT CTGCACATGC TCCCAGCTGA GCTTCAGCGA GGAGACCAAG 
CAGGTGTTGC GTCTCAGTCT GACCGAGTTC TCCCTGCCGA AGTTCTACCT GCTGTTCGGC 
ATCAGCAAGG TCAAGTCGGC TGCGGTCATT GCATTTCGTT CCAGCTACAG GGATCGCTCA 
TTCTCGCCGG GGTCCTATGA GAGAGAGACC TTTGCTCTGT CATCTCTGGA GACGGTCACA 
GAGGCGCTGC TGGAGATCAT GGAGGCTTGC ATGAGGGACA TTCCAGGCTG TAAATGGCTA 
GACCAGTGGA CCGATCTGGC GCAGAAGTTT GCATTTCAAT ATAATCCATC CCTCCAACCA 
CGGGCACTTG TGGTATTCGG CTGCATCAGC AAACGAGTCA CACATACGCA GATGAAACAG 
ATCATACGGA TCTTGAGCAA GGCCAGCCTT CTCCTCAGCA TCGACCGGGG TTTGGAGAGC 
TGCCTGAAGG GTCCCGATAA CTGTAACAGC CAGGTGCTAA TCGAAGCCAC AGTCATTGCT 
CTCACTAAAC TACAACCTTT GCTCAACAAG GACTCAGCGA TGCACAAAGC TCTGTTCTGG 
GTCGCTGTGG CCGTACTGCA GTTGGATGAG GTGAATCTGT ATTCTGCAGG AACTGCTCTG 
CTTGAACAGA ACCTACACAC ACTCGATAGC CTGAGAGTCT TCAACGACAA GAGTCCTGAG 
GAGGTGTTTA TGGAGATCCG TCAGCCTCTG GAATGGCACT GTAAACAAAT GGACCACTTT 
GTTGGCCTCA ATTTTAACTC CAACTTCAAT TTTGCTTTGG TCGGTCACCT GTTGAAAGGG 
TTCAGACACC CCTCCCCCAC CACAGTGGCA CGTACCATCC GCATCCTGCA CATGCTGTTG 
GCGTTAGTAG GAAAACACCA GAACTGTGAT AAATTTGAGG TGAACACACA GAGTGTGGCG 
TATCTGGCAG CGCTTCTCCC GGTGTCAGAG GAAGTGCGCA GCCGCTGCAG TCTGAAACAC 
AGAAAATTGT TGCTTTTCTC TGATGTCACA ATGGAGAATG TTCAAATGGA TCCGTATTCT 
GTACATCACG AGGATCCAAG CGGCAGAACG TTGCGGGAAA ACGAGCTGTG GGCATCCCCG 
AAAGTCTCAG AGCGATACTT GGTGGCCCAT TTCCCCATAA TGGGGCAGAT CAGCCCACGA 
ACACGCAAAT CAATGAGCTT GGACATGGGA CAACCCTCTC AAGTCAACAC CAAGAAGCTT 
CTGGGCACCA GAAAAAGCTT TGACCTCCTG TTATCAGACA GCAAAGCACC AAAGAGGCCT 
GAAATAGAGA CTGGTATCAC AACGCCACCC AAGATGAGGA GAGTGGCTGA AAACGAATAT 
GAAATCGAAA CCCCAAGAAT CTCCCATCAC CACTTGCGGA AGGTTTCTGT GTCCGAGTCC 
AATGTCCTGC TTAATGAAGA AGTTCTCACA GATCCCAAAA TCCAAGCACT GCTTCTGACT 
GTGCTGGCCA CTCAGGTGAA AAACACTTCA GATGAATTTG AGCAGAGGAT CTTGTATGAG 
TTTCTAGCCG AGGCCAGTGT TGTCTTCCCA AAAGTCTTTC CTGTTGTTCA TAATCTTCTG 
GACTCTAAGA TTAATACGTT GTTGTCGCTG TGTCAGGACC CACATCTTCT GAATCCAGTT 
CATGGAATTG TTCAGAGTGT AGTTTATCAT GAAGAGTCTC CACCTCAGTA CCAACCGTCC 
TACCTGCAAA CTTTTGGGTT TAATGGACTC TGGAGGTTTG CCGGTCCTTT TTCGAAGCAA 
ACTCAGTTCC CAGACTATGC AGAGCTGATA GTCAAGTTTC TGGATGCACT GATTGACACC 
TATCTTCCGG GCATTGATGA GGAAACAAGC GAGGAGGGTC TGCGGACACC CACCTCTCCA 
TACCCTCCAC CTGTGCAGAG CCAGCTCAGC ATCACTGCCA ACCTCAACCT CTCAAATTCA 
ATGACCTCGC TGGCCACCTC CCAACATGCA CCAGGAATGG ATAAAGAGAA AGTGGATCTA 
TCCCCCACCA CGGGTTTGGC AGCCGGCGGA CGCACACGCC ACGGCTCGGC CAGCCAGGTC 
CAGAAACAGC GCAGCACCGG CAGCTTTAAG AGACCTAGCA TTAAAAAGAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

nf1b cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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